Feed for adults of Meloidogyne eyrei and preparation method thereof

Abstract

The present invention discloses a kind of eye-spotted meloides adult feed and its preparation method, belong to the field of insect breeding technology. The eye-spotted meloides adult feed includes: 11-15 parts of casein, 1-3 parts of Wei's salt, 4-6 parts of sucrose, 9-12 parts of cellulose powder, 0.2-0.4 parts of L-cysteine, 0.2-0.3 parts of choline chloride, 0.08-0.1 parts of inositol, 0.325-0.4 parts of multivitamins, 2-3 parts of nutrient ethanol solution, 12-14 parts of nutrient aqueous solution, 1-2 parts of honey, 2-3 parts of agar powder, 0.03-0.04 parts of cholesterol, 33-67 parts of applesauce, 0.3-4 parts of royal jelly and 80-120 parts of water. In addition, the present invention also proposes a preparation method of the above-mentioned eye-spotted meloides adult feed. The eye-spotted meloides adult feed proposed by the present invention can prolong the lifespan of adults while increasing egg production.

Description

Technical Field

[0001] The invention relates to the technical field of insect breeding, and in particular to a feed for adult melia odorata and a preparation method thereof. Background Art

[0002] Mylabris cichorii is an important medicinal insect. It contains cantharidin, a compound with anti-cancer properties, and therefore holds great potential in pharmaceutical applications. However, traditional breeding of Mylabris cichorii relies primarily on natural food sources, such as locust eggs for larvae and plants like loofah flowers for adults. This breeding method has numerous limitations, including high costs, seasonal restrictions, and difficulty in scaling.

[0003] To overcome these limitations, researchers have recently begun exploring artificial feed formulations for the scaly-eyed meliad. For example, one study developed an artificial feed for adults containing soybean powder, yeast powder, dried loofah flowers, multivitamin glucose, pollen, and honey. This feed not only replaces natural loofah flowers but also meets the nutritional needs of adult scaly-eyed meliads, significantly reducing feeding costs.

[0004] Although existing research has achieved certain results, the existing technology still has shortcomings. For example, some artificial feed formulas still need to add a small amount of natural ingredients (such as dried loofah flowers). Natural dried loofah flowers and other natural ingredients are difficult to mass-produce, which to a certain extent limits the scale and cost control of breeding. In addition, how to further extend the lifespan of adults and increase egg production remains a key issue in current research. Summary of the Invention

[0005] This invention aims to provide a novel feed for adult Meloidogyne spp. and its preparation method to address existing issues and further promote the large-scale cultivation of Meloidogyne spp. and the stable production of cantharidin. By optimizing the feed formula, the present invention aims to significantly extend the lifespan of adult Meloidogyne spp. and increase egg production, providing technical support for the efficient rearing of adult Meloidogyne spp.

[0006] The purpose of the present invention is to overcome the above technical deficiencies, provide a feed for adults of Meloidogyne spp. and a preparation method thereof, and solve the technical problems in the prior art of how to prolong the lifespan of adults and increase the amount of eggs laid.

[0007] In order to achieve the above technical objectives, the technical solution of the present invention provides a feed for adult meloides, which comprises, calculated by weight, 11 to 15 parts of casein, 1 to 3 parts of Webster's salt, 4 to 6 parts of sucrose, 9 to 12 parts of cellulose powder, 0.2 to 0.4 parts of L-cysteine, 0.2 to 0.3 parts of choline chloride, 0.08 to 0.1 parts of inositol, 0.325 to 0.4 parts of multivitamins, 2 to 3 parts of ethanol solution of nutrients, 0.10 to 0.13 parts of linoleic acid, 0.05 to 0.07 parts of linolenic acid, 12 to 14 parts of aqueous solution of nutrients, 1 to 2 parts of honey, 2 to 3 parts of agar powder, 0.03 to 0.04 parts of cholesterol, 33 to 67 parts of applesauce, 0.3 to 4 parts of royal jelly and 80 to 120 parts of water.

[0008] In any embodiment, the composition further comprises 0.7 to 1 parts of ascorbic acid and 0.2 to 0.4 parts of sorbic acid.

[0009] In any embodiment, the ethanol solution of nutrients includes: 0.01 to 0.03 parts of dl-α-tocopheryl acetate, 0.001 to 0.002 parts of vitamin D3, and 0.001 to 0.002 parts of menadione.

[0010] In any embodiment, the aqueous nutrient solution includes: 0.002-0.003 parts of niacinamide, 0.002-0.003 parts of calcium D-pantothenate, 0.0005-0.0007 parts of thiamine, 0.001-0.002 parts of riboflavin, 0.0005-0.0007 parts of pyridoxine, 0.0005-0.0007 parts of folic acid, 0.00004-0.00006 parts of D-biotin, 0.000004-0.000006 parts of vitamin B12, 0.001-0.002 parts of anhydrous zinc acetate, 0.0005-0.0007 parts of anhydrous cobalt chloride, and 0.0005-0.0007 parts of anhydrous sodium molybdate.

[0011] In addition, the present invention also provides a method for preparing the above-mentioned adult feed of the eye-spotted meloides, comprising the following steps:

[0012] S1, dissolving cholesterol in chloroform, then adding casein and cellulose powder and mixing them, and then drying to obtain a first mixed material;

[0013] S2. Mixing the first mixed material, Webster's salt, sucrose, L-cysteine, choline chloride, inositol, multivitamins, ethanol solution of nutrients, linoleic acid, linolenic acid, aqueous solution of nutrients, honey, applesauce, royal jelly, and part of the water to obtain a second mixed material; mixing agar powder and the remaining water and dissolving them at 90-100° C. to obtain an agar solution;

[0014] S3. Mixing the second mixed material and the agar solution to obtain the adult feed of the Meloidogyne spp.

[0015] In any embodiment, in step S2, the nutrient component ethanol solution is prepared by the following steps: dissolving dl-α-tocopheryl acetate, vitamin D3 and menadione in ethanol to obtain the nutrient component ethanol solution.

[0016] In any embodiment, in step S2, the nutrient solution is prepared by dissolving nicotinamide, D-calcium pantothenate, thiamine, riboflavin, pyridoxine, folic acid, D-biotin, vitamin B12, anhydrous zinc acetate, anhydrous cobalt chloride and anhydrous sodium molybdate in water to obtain the nutrient solution.

[0017] In any embodiment, in step S2, the amount of water added to the second mixed material is 15% to 40% of the total weight of the water component added to the feed.

[0018] In any embodiment, in step S1, the nutrient component ethanol solution or the nutrient component aqueous solution is stored at 3-5°C for later use.

[0019] In any embodiment, step S2 further includes adding ascorbic acid and sorbic acid to the first mixed material, Webster salt, sucrose, L-cysteine, choline chloride, inositol, multivitamins, ethanol solution of nutrients, linoleic acid, linolenic acid, aqueous solution of nutrients, honey, applesauce, royal jelly and part of water to obtain the second mixed material.

[0020] Compared with the prior art, the present invention has the following beneficial effects: the adult feed for the eye-spotted meloides proposed in the present invention can significantly prolong the lifespan of adult eye-spotted meloides by optimizing its nutritional components, particularly by adding multiple vitamins, amino acids, and essential fatty acids. The feed is rich in nutrients, such as vitamin D3, B vitamins, cholesterol, and royal jelly, which can significantly increase the egg production of adult insects. The feed formula includes multiple nutrients such as protein, carbohydrates, vitamins, minerals, and essential fatty acids, which can meet the growth needs of adult eye-spotted meloides, ultimately extending the lifespan of adults while increasing egg production.

[0021] In addition, compared with traditional feeding methods that rely on natural ingredients (such as loofah flowers and locust eggs), the artificial feed proposed in this invention reduces the time and manpower required for planting, picking and preserving natural ingredients. All of the above provides strong support for the large-scale breeding of adult melonids and the stable production of cantharidin. DETAILED DESCRIPTION

[0022] The "ranges" disclosed herein are defined in terms of lower and upper limits, where a given range is defined by selecting a lower limit and an upper limit, and the selected lower and upper limits define the boundaries of the particular range. Ranges defined in this manner can be inclusive or exclusive of the end values and can be combined arbitrarily, i.e., any lower limit can be combined with any upper limit to form a range. For example, if ranges of 60 to 120 and 80 to 110 are listed for a particular parameter, it is understood that ranges of 60 to 110 and 80 to 120 are also contemplated. Furthermore, if minimum range values of 1 and 2 are listed, and if maximum range values of 3, 4, and 5 are listed, the following ranges are all contemplated: 1 to 3, 1 to 4, 1 to 5, 2 to 3, 2 to 4, and 2 to 5. In this application, unless otherwise indicated, the numerical range "a to b" is a shorthand representation of any combination of real numbers between a and b, where a and b are both real numbers. For example, a numerical range of "0-5" indicates that all real numbers between "0-5" are listed herein, and "0-5" is simply an abbreviation for these numerical combinations. Furthermore, when a parameter is expressed as an integer ≥ 2, this is equivalent to disclosing that the parameter is, for example, an integer of 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, etc.

[0023] Unless otherwise specified, the terms "include" and "comprising" used in this application may be open-ended or closed-ended. For example, "include" and "comprising" may mean that other components not listed may also be included or that only the listed components are included.

[0024] Unless otherwise specified, the term "or" is used in this application to be inclusive. For example, the phrase "A or B" means "A, B, or both A and B." More specifically, the condition "A or B" is satisfied if any of the following conditions are met: A is true (or exists) and B is false (or does not exist); A is false (or does not exist) and B is true (or exists); or both A and B are true (or exist).

[0025] This specific embodiment provides a feed for adult meloides, which includes, by weight, 11-15 parts of casein, 1-3 parts of Webster's salt, 4-6 parts of sucrose, 9-12 parts of cellulose powder, 0.2-0.4 parts of L-cysteine, 0.2-0.3 parts of choline chloride, 0.08-0.1 parts of inositol, 0.325-0.4 parts of multivitamins, 2-3 parts of ethanol solution of nutrients, 0.10-0.13 parts of linoleic acid, 0.05-0.07 parts of linolenic acid, 12-14 parts of aqueous solution of nutrients, 1-2 parts of honey, 2-3 parts of agar powder, 0.03-0.04 parts of cholesterol, 33-67 parts of applesauce, 0.3-4 parts of royal jelly and 80-120 parts of water.

[0026] In some embodiments, 0.7-1 parts of ascorbic acid and 0.2-0.4 parts of sorbic acid are also included. The added ascorbic acid and sorbic acid can extend the shelf life of the feed and ensure the stability of the nutritional components.

[0027] In some embodiments, the ethanolic nutrient solution includes: 0.02-0.03 parts of dl-α-tocopheryl acetate, 0.001-0.002 parts of vitamin D3, and 0.001-0.002 parts of menadione. The weight percentages of the components of the ethanolic nutrient solution are calculated relative to the total weight percentages of the adult feed for the scaly-spotted meloides, and are the weight percentages of the 2-3 parts of the ethanolic nutrient solution. The sum of the weight percentages of the components in the ethanolic nutrient solution and the weight percentages of water therein is 2-3 parts.

[0028] In some embodiments, the aqueous solution of nutrients includes: 0.002-0.003 parts of niacinamide, 0.002-0.003 parts of D-calcium pantothenate, 0.0005-0.0007 parts of thiamine, 0.001-0.002 parts of riboflavin, 0.0005-0.0007 parts of pyridoxine, 0.0005-0.0007 parts of folic acid, 0.00004-0.00006 parts of D-biotin, 0.000004-0.000006 parts of vitamin B12, 0.001-0.002 parts of anhydrous zinc acetate, 0.0005-0.0007 parts of anhydrous cobalt chloride, and 0.0005-0.0007 parts of anhydrous sodium molybdate. The weight proportions of the components of the nutrient solution are calculated relative to the total weight proportions of the adult feed of the eye-spotted melon, and are the weight proportions in 12 to 14 parts of the nutrient solution. The sum of the weight proportions of the components in the nutrient solution and the weight proportions of water therein is 12 to 14 parts.

[0029] This specific embodiment also proposes a method for preparing the above-mentioned adult feed of Meloidogyne ocellaris, comprising the following steps:

[0030] S1, dissolving cholesterol in chloroform, then adding casein and cellulose powder and mixing them, and then drying to obtain a first mixed material;

[0031] S2. The first mixed material, Webster's salt, sucrose, L-cysteine, choline chloride, inositol, multivitamins, nutrient ethanol solution, linoleic acid, linolenic acid, nutrient aqueous solution, honey, applesauce, royal jelly and part of the water are mixed to obtain a second mixed material; agar powder and the remaining water are mixed and dissolved at 90-100° C. to obtain an agar solution; the nutrient ethanol solution is prepared by the following steps: dl-α-tocopheryl acetate, vitamin D3 and menadione are dissolved in ethanol to obtain the nutrient ethanol solution; the nutrient aqueous solution is prepared by the following steps Nicotinamide, D-calcium pantothenate, thiamine, riboflavin, pyridoxine, folic acid, D-biotin, vitamin B12, anhydrous zinc acetate, anhydrous cobalt chloride, and anhydrous sodium molybdate are dissolved in water to obtain the nutrient solution; the amount of water added to the second mixed material is 15% to 40% of the total weight of the water component added to the feed, where the water component refers to 80 to 120 parts of water, and the sum of the remaining water after the water added to the second mixed material and the agar powder is 80 to 120 parts; the nutrient ethanol solution or the nutrient solution is stored at 3-5°C for future use.

[0032] S3. Mixing the second mixed material and the agar solution to obtain the adult feed of the Meloidogyne spp.

[0033] In some embodiments, the method further includes adding ascorbic acid and sorbic acid to the first mixed material, Webster salt, sucrose, L-cysteine, choline chloride, inositol, multivitamins, ethanol solution of nutrients, linoleic acid, linolenic acid, aqueous solution of nutrients, honey, applesauce, royal jelly and part of water to obtain the second mixed material.

[0034] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0035] In the present invention, references to “some embodiments”, “this embodiment”, examples, etc. describe a subset of all possible embodiments, but it can be understood that “some embodiments” may be the same subset or different subsets of all possible embodiments, and may be combined with each other without conflict.

[0036] If similar descriptions of "first / second" appear in the application documents, the following explanation is added. In the following description, the terms "first\second\third" are merely used to distinguish similar objects and do not represent a specific order of the objects. It can be understood that "first\second\third" can be interchanged with the specific order or sequence where permitted, so that the embodiments described herein can be implemented in an order other than that described herein.

[0037] In this embodiment, the term "and / or" is merely a description of the association relationship between associated objects, indicating that three relationships may exist. For example, object A and / or object B may represent three situations: object A exists alone, object A and object B exist at the same time, and object B exists alone.

[0038] It should be noted that the multivitamin is called Vitamin mix, which is a single product and is purchased under the name: Vanderzant Vitamin Mix (for Insects) | Sigma-Aldrich.

[0039] Example 1

[0040] This embodiment provides a feed for adult worms of the eye-spotted meloides, which comprises, by weight, 12 parts of casein, 2 parts of Webster's salt, 4 parts of sucrose, 10 parts of cellulose powder, 0.2 parts of L-cysteine, 0.2 parts of choline chloride, 0.08 parts of inositol, 0.325 parts of multivitamins (i.e., Vitamin Mix), 2 parts of nutrient ethanol solution, 0.11 parts of linoleic acid, 0.054 parts of linolenic acid, 12 parts of nutrient aqueous solution, 0.8 parts of ascorbic acid, 0.2 parts of sorbic acid, 1 part of honey, 2 parts of agar powder, 0.035 parts of cholesterol, 50 parts of applesauce, 2 parts of royal jelly, and 100 parts of water; the nutrient ethanol solution comprises: 0.02 parts of dl-α-tocopheryl acetate, 0.03 parts of vitamin D3 0.001 part, and 0.001 part of menadione; the aqueous solution of nutrients includes: 0.002 part of niacinamide, 0.002 part of D-calcium pantothenate, 0.0005 part of thiamine, 0.001 part of riboflavin, 0.0005 part of pyridoxine, 0.0005 part of folic acid, 0.00004 part of D-biotin, 0.000004 part of vitamin B12, 0.001 part of anhydrous zinc acetate, 0.0005 part of anhydrous cobalt chloride, and 0.0005 part of anhydrous sodium molybdate.

[0041] This embodiment also provides a method for preparing the above-mentioned adult feed of Meloidogyne ocellaris, comprising the following steps:

[0042] S0, dissolving dl-α-tocopheryl acetate, vitamin D3 and menadione in ethanol to obtain the nutrient ethanol solution; dissolving nicotinamide, D-calcium pantothenate, thiamine, riboflavin, pyridoxine, folic acid, D-biotin, vitamin B12, anhydrous zinc acetate, anhydrous cobalt chloride and anhydrous sodium molybdate in water to obtain the nutrient aqueous solution; the nutrient ethanol solution or the nutrient aqueous solution is stored at 4° C. for future use;

[0043] S1. dissolving cholesterol in chloroform, then adding casein and cellulose powder to mix with the mixture, and then drying to obtain a first mixed material;

[0044] S2. Mix the first mixed material, Webster's salt, sucrose, L-cysteine, choline chloride, inositol, multivitamins, nutrient ethanol solution, linoleic acid, linolenic acid, nutrient aqueous solution, honey, applesauce, royal jelly, and 30 mL of water to obtain a second mixed material; mix agar powder and the remaining 70 mL of water and dissolve them at 100° C. to obtain an agar solution;

[0045] S3. Mixing the second mixed material and the agar solution to obtain the adult feed of the Meloidogyne spp.

[0046] Example 2

[0047] This embodiment provides a feed for adult worms of the eye-spotted meloides, which comprises, by weight, 12 parts of casein, 2 parts of Webster's salt, 4 parts of sucrose, 10 parts of cellulose powder, 0.2 parts of L-cysteine, 0.2 parts of choline chloride, 0.08 parts of inositol, 0.325 parts of multivitamins, 2 parts of ethanol solution of nutrients, 0.11 parts of linoleic acid, 0.054 parts of linolenic acid, 12 parts of aqueous solution of nutrients, 0.8 parts of ascorbic acid, 0.2 parts of sorbic acid, 1 part of honey, 2 parts of agar powder, and 0.037 parts of cholesterol. parts, 60 parts of applesauce, 1 part of royal jelly and 90 parts of water; the nutrient ingredient ethanol solution includes: 0.02 parts of dl-α-tocopheryl acetate, 0.001 parts of vitamin D3, and 0.001 parts of menadione; the nutrient ingredient aqueous solution includes: 0.002 parts of niacinamide, 0.002 parts of D-calcium pantothenate, 0.0005 parts of thiamine, 0.001 parts of riboflavin, 0.0005 parts of pyridoxine, 0.0005 parts of folic acid, 0.00004 parts of D-biotin, 0.000004 parts of vitamin B12, 0.001 parts of anhydrous zinc acetate, 0.0005 parts of anhydrous cobalt chloride and 0.0005 parts of anhydrous sodium molybdate.

[0048] This embodiment also provides a method for preparing the above-mentioned adult feed of Meloidogyne ocellaris, comprising the following steps:

[0049] S0, dissolving dl-α-tocopheryl acetate, vitamin D3 and menadione in ethanol to obtain the nutrient ethanol solution; dissolving nicotinamide, D-calcium pantothenate, thiamine, riboflavin, pyridoxine, folic acid, D-biotin, vitamin B12, anhydrous zinc acetate, anhydrous cobalt chloride and anhydrous sodium molybdate in water to obtain the nutrient aqueous solution; the nutrient ethanol solution or the nutrient aqueous solution is stored at 4° C. for future use;

[0050] S1. dissolving cholesterol in chloroform, then adding casein and cellulose powder to mix with the mixture, and then drying to obtain a first mixed material;

[0051] S2. Mix the first mixed material, Webster's salt, sucrose, L-cysteine, choline chloride, inositol, multivitamins, ethanol solution of nutrients, linoleic acid, linolenic acid, aqueous solution of nutrients, honey, applesauce, royal jelly, and 20 mL of water to obtain a second mixed material; mix agar powder and the remaining 70 mL of water and dissolve them at 90° C. to obtain an agar solution;

[0052] S3. Mixing the second mixed material and the agar solution to obtain the adult feed of the Meloidogyne spp.

[0053] Example 3

[0054] This embodiment provides a feed for adult worms of the eye-spotted meloides, which comprises, by weight, 12 parts of casein, 2 parts of Webster's salt, 4 parts of sucrose, 10 parts of cellulose powder, 0.2 parts of L-cysteine, 0.2 parts of choline chloride, 0.08 parts of inositol, 0.325 parts of multivitamins, 2 parts of ethanol solution of nutrients, 0.11 parts of linoleic acid, 0.054 parts of linolenic acid, 12 parts of aqueous solution of nutrients, 0.8 parts of ascorbic acid, 0.2 parts of sorbic acid, 1 part of honey, 2 parts of agar powder, and 0.033 parts of cholesterol. parts, 60 parts of applesauce, 3 parts of royal jelly and 90 parts of water; the nutrient ingredient ethanol solution includes: 0.02 parts of dl-α-tocopheryl acetate, 0.001 parts of vitamin D3, and 0.001 parts of menadione; the nutrient ingredient aqueous solution includes: 0.002 parts of niacinamide, 0.002 parts of D-calcium pantothenate, 0.0005 parts of thiamine, 0.001 parts of riboflavin, 0.0005 parts of pyridoxine, 0.0005 parts of folic acid, 0.00004 parts of D-biotin, 0.000004 parts of vitamin B12, 0.001 parts of anhydrous zinc acetate, 0.0005 parts of anhydrous cobalt chloride and 0.0005 parts of anhydrous sodium molybdate.

[0055] This embodiment also provides a method for preparing the above-mentioned adult feed of Meloidogyne ocellaris, comprising the following steps:

[0056] S0, dissolving dl-α-tocopheryl acetate, vitamin D3 and menadione in ethanol to obtain the nutrient ethanol solution; dissolving nicotinamide, D-calcium pantothenate, thiamine, riboflavin, pyridoxine, folic acid, D-biotin, vitamin B12, anhydrous zinc acetate, anhydrous cobalt chloride and anhydrous sodium molybdate in water to obtain the nutrient aqueous solution; the nutrient ethanol solution or the nutrient aqueous solution is stored at 4° C. for future use;

[0057] S1. dissolving cholesterol in chloroform, then adding casein and cellulose powder to mix with the mixture, and then drying to obtain a first mixed material;

[0058] S2. Mix the first mixed material, Webster's salt, sucrose, L-cysteine, choline chloride, inositol, multivitamins, nutrient ethanol solution, linoleic acid, linolenic acid, nutrient aqueous solution, honey, applesauce, royal jelly, and 20 mL of water to obtain a second mixed material; mix agar powder and the remaining 70 mL of water and dissolve them at 95° C. to obtain an agar solution; the amount of water added to the second mixed material is 40% of the total weight of the water component added to the feed;

[0059] S3. Mixing the second mixed material and the agar solution to obtain the adult feed of the Meloidogyne spp.

[0060] Example 4

[0061] This embodiment provides a feed for adult worms of the eye-spotted meloides, which comprises, by weight, 12 parts of casein, 2 parts of Webster's salt, 4 parts of sucrose, 10 parts of cellulose powder, 0.2 parts of L-cysteine, 0.2 parts of choline chloride, 0.08 parts of inositol, 0.325 parts of multivitamins, 2 parts of ethanol solution of nutrients, 0.11 parts of linoleic acid, 0.054 parts of linolenic acid, 12 parts of aqueous solution of nutrients, 0.8 parts of ascorbic acid, 0.2 parts of sorbic acid, 1 part of honey, 2 parts of agar powder, and 0.037 parts of cholesterol. parts, 40 parts of applesauce, 3 parts of royal jelly and 90 parts of water; the nutrient ingredient ethanol solution includes: 0.02 parts of dl-α-tocopheryl acetate, 0.001 parts of vitamin D3, and 0.001 parts of menadione; the nutrient ingredient aqueous solution includes: 0.002 parts of niacinamide, 0.002 parts of D-calcium pantothenate, 0.0005 parts of thiamine, 0.001 parts of riboflavin, 0.0005 parts of pyridoxine, 0.0005 parts of folic acid, 0.00004 parts of D-biotin, 0.000004 parts of vitamin B12, 0.001 parts of anhydrous zinc acetate, 0.0005 parts of anhydrous cobalt chloride and 0.0005 parts of anhydrous sodium molybdate.

[0062] This embodiment also provides a method for preparing the above-mentioned adult feed of Meloidogyne ocellaris, comprising the following steps:

[0063] S0, dissolving dl-α-tocopheryl acetate, vitamin D3 and menadione in ethanol to obtain the nutrient ethanol solution; dissolving nicotinamide, D-calcium pantothenate, thiamine, riboflavin, pyridoxine, folic acid, D-biotin, vitamin B12, anhydrous zinc acetate, anhydrous cobalt chloride and anhydrous sodium molybdate in water to obtain the nutrient aqueous solution; the nutrient ethanol solution or the nutrient aqueous solution is stored at 4° C. for future use;

[0064] S1. dissolving cholesterol in chloroform, then adding casein and cellulose powder to mix with the mixture, and then drying to obtain a first mixed material;

[0065] S2. Mix the first mixed material, Webster's salt, sucrose, L-cysteine, choline chloride, inositol, multivitamins, nutrient ethanol solution, linoleic acid, linolenic acid, nutrient aqueous solution, honey, applesauce, royal jelly, and 20 mL of water to obtain a second mixed material; mix agar powder and the remaining 70 mL of water and dissolve them at 95° C. to obtain an agar solution; the amount of water added to the second mixed material is 25% of the total weight of the water component added to the feed;

[0066] S3. Mixing the second mixed material and the agar solution to obtain the adult feed of the Meloidogyne spp.

[0067] Example 5

[0068] This embodiment provides a feed for adult worms of the eye-spotted meloides, which comprises, by weight, 12 parts of casein, 2 parts of Webster's salt, 4 parts of sucrose, 10 parts of cellulose powder, 0.2 parts of L-cysteine, 0.2 parts of choline chloride, 0.08 parts of inositol, 0.325 parts of multivitamins, 2 parts of nutrient ethanol solution, 0.11 parts of linoleic acid, 0.054 parts of linolenic acid, 12 parts of nutrient aqueous solution, 0.8 parts of ascorbic acid, 0.2 parts of sorbic acid, 1 part of honey, 2 parts of agar powder, 0.0316364 parts of cholesterol, 50 parts of applesauce, 2 parts of royal jelly, and 100 parts of water; the nutrient ethanol solution comprises: 0.02 parts of dl-α-tocopheryl acetate, vitamin D3 0.001 part, and 0.001 part of menadione; the aqueous solution of nutrients includes: 0.002 part of niacinamide, 0.002 part of D-calcium pantothenate, 0.0005 part of thiamine, 0.001 part of riboflavin, 0.0005 part of pyridoxine, 0.0005 part of folic acid, 0.00004 part of D-biotin, 0.000004 part of vitamin B12, 0.001 part of anhydrous zinc acetate, 0.0005 part of anhydrous cobalt chloride, and 0.0005 part of anhydrous sodium molybdate.

[0069] This embodiment also provides a method for preparing the above-mentioned adult feed of Meloidogyne ocellaris, comprising the following steps:

[0070] S0, dissolving dl-α-tocopheryl acetate, vitamin D3 and menadione in ethanol to obtain the nutrient ethanol solution; dissolving nicotinamide, D-calcium pantothenate, thiamine, riboflavin, pyridoxine, folic acid, D-biotin, vitamin B12, anhydrous zinc acetate, anhydrous cobalt chloride and anhydrous sodium molybdate in water to obtain the nutrient aqueous solution; the nutrient ethanol solution or the nutrient aqueous solution is stored at 4° C. for future use;

[0071] S1. dissolving cholesterol in chloroform, then adding casein and cellulose powder to mix with the mixture, and then drying to obtain a first mixed material;

[0072] S2. Mix the first mixed material, Webster's salt, sucrose, L-cysteine, choline chloride, inositol, multivitamins, ethanol solution of nutrients, linoleic acid, linolenic acid, aqueous solution of nutrients, honey, applesauce, royal jelly, and 30 mL of water to obtain a second mixed material; mix agar powder and the remaining 70 mL of water and dissolve them at 95° C. to obtain an agar solution; the amount of water added to the second mixed material is 25% of the total weight of the water component added to the feed;

[0073] S3. Mixing the second mixed material and the agar solution to obtain the adult feed of the Meloidogyne spp.

[0074] Example 6

[0075] This embodiment provides a feed for adult worms of the eye-spotted meloides, which comprises, by weight, 12 parts of casein, 2 parts of Webster's salt, 4 parts of sucrose, 10 parts of cellulose powder, 0.2 parts of L-cysteine, 0.2 parts of choline chloride, 0.08 parts of inositol, 0.325 parts of multivitamins, 2 parts of nutrient ethanol solution, 0.11 parts of linoleic acid, 0.054 parts of linolenic acid, 12 parts of nutrient aqueous solution, 0.8 parts of ascorbic acid, 0.2 parts of sorbic acid, 1 part of honey, 2 parts of agar powder, 0.0383636 parts of cholesterol, 50 parts of applesauce, 2 parts of royal jelly, and 100 parts of water; the nutrient ethanol solution comprises: 0.02 parts of dl-α-tocopheryl acetate, vitamin D3 0.001 part, and 0.001 part of menadione; the aqueous solution of nutrients includes: 0.002 part of niacinamide, 0.002 part of D-calcium pantothenate, 0.0005 part of thiamine, 0.001 part of riboflavin, 0.0005 part of pyridoxine, 0.0005 part of folic acid, 0.00004 part of D-biotin, 0.000004 part of vitamin B12, 0.001 part of anhydrous zinc acetate, 0.0005 part of anhydrous cobalt chloride, and 0.0005 part of anhydrous sodium molybdate.

[0076] This embodiment also provides a method for preparing the above-mentioned adult feed of Meloidogyne ocellaris, comprising the following steps:

[0077] S0, dissolving dl-α-tocopheryl acetate, vitamin D3 and menadione in ethanol to obtain the nutrient ethanol solution; dissolving nicotinamide, D-calcium pantothenate, thiamine, riboflavin, pyridoxine, folic acid, D-biotin, vitamin B12, anhydrous zinc acetate, anhydrous cobalt chloride and anhydrous sodium molybdate in water to obtain the nutrient aqueous solution; the nutrient ethanol solution or the nutrient aqueous solution is stored at 4° C. for future use;

[0078] S1. dissolving cholesterol in chloroform, then adding casein and cellulose powder to mix with the mixture, and then drying to obtain a first mixed material;

[0079] S2. Mix the first mixed material, Webster's salt, sucrose, L-cysteine, choline chloride, inositol, multivitamins, nutrient ethanol solution, linoleic acid, linolenic acid, nutrient aqueous solution, honey, applesauce, royal jelly, and 30 mL of water to obtain a second mixed material; mix agar powder and the remaining 70 mL of water and dissolve them at 95° C. to obtain an agar solution; the amount of water added to the second mixed material is 25% of the total weight of the water component added to the feed;

[0080] S3. Mixing the second mixed material and the agar solution to obtain the adult feed of the Meloidogyne spp.

[0081] Comparative Example 1

[0082] The difference between the adult feed of Meloidogyne spp. in this comparative example and that in the embodiment is that the feed used is natural artificial feed.

[0083] The natural artificial feed contains the following components: 100 ml of water, 36 g of soybean powder, 1 g of honey, 1 g of royal jelly, and 1.6 g of agar powder.

[0084] The preparation method of the eye spot meloides adult feed of this comparative example is as follows:

[0085] S0. Sterilize the soybean powder at 121° C. for 30 min to obtain a sterilized material.

[0086] S1. Mix the sterilized material obtained in step S0 with royal jelly, honey and 50 ml of water to obtain a mixed material.

[0087] S2. Dissolve the agar powder and the remaining water at 100° C., stir the obtained mixed solution with the mixed material in step S1, and cool the mixture to obtain the artificial feed.

[0088] Related trials

[0089] Adult 35 male and female Meloidogyne spp. were divided into seven groups (Examples 1-6 and Comparative Example 1), each with five males and five females. The insects were housed in an incubator at a constant temperature of 30°C and a 16-hour light cycle in seven transparent containers containing sand and soil at a sand-to-soil ratio of 7:3. The feed was changed daily at 10:00 AM. The average daily feed intake per individual, average adult lifespan, pre-oviposition period, egg production, and average egg number for Examples 1-6 and Comparative Example 1 are shown in Table 1.

[0090] Table 1

[0091]

[0092] As can be seen from Table 1, the average daily food intake of each head in the examples and the comparative example is not much different, but the average lifespan, egg production and average egg number of the adults of the eye-spotted meloides fed with the feeds of Examples 1-6 are relatively high, and the pre-oviposition period is relatively short, while the average lifespan, egg production and average egg number of the adults of the eye-spotted meloides fed with the feeds of the comparative example are relatively low, and the pre-oviposition period is long. It can be seen that the feeds of Examples 1-6 can significantly increase the average lifespan, egg production and average egg number of the adults of the eye-spotted meloides, and can shorten the pre-oviposition period.

[0093] The specific embodiments of the present invention described above do not limit the scope of protection of the present invention. Any other corresponding changes and modifications made based on the technical concept of the present invention should be included in the scope of protection of the claims of the present invention.

Claims

1. A feed for adults of Meloidogyne sphenanthera, characterized in that: The invention comprises, by weight, 11-15 parts of casein, 1-3 parts of Webster's salt, 4-6 parts of sucrose, 9-12 parts of cellulose powder, 0.2-0.4 parts of L-cysteine, 0.2-0.3 parts of choline chloride, 0.08-0.1 parts of inositol, 0.325-0.4 parts of multivitamins, 2-3 parts of ethanol solution of nutrients, 0.10-0.13 parts of linoleic acid, 0.05-0.07 parts of linolenic acid, 12-14 parts of aqueous solution of nutrients, 1-2 parts of honey, 2-3 parts of agar powder, 0.03-0.04 parts of cholesterol, 33-67 parts of applesauce, 0.3-4 parts of royal jelly and 80-120 parts of water. The nutrient ethanol solution comprises: 0.01-0.03 parts of dl-α-tocopheryl acetate, 0.001-0.002 parts of vitamin D3, 0.001-0.002 parts of menadione, and the balance is ethanol; the weight parts of each component of the nutrient ethanol solution are calculated relative to the total weight parts of the eye-spotted meloides adult feed, and are the weight parts of the 2-3 parts of the nutrient ethanol solution; The nutrient solution comprises: 0.002-0.003 parts of nicotinamide, 0.002-0.003 parts of D-calcium pantothenate, 0.0005-0.0007 parts of thiamine, 0.001-0.002 parts of riboflavin, 0.0005-0.0007 parts of pyridoxine, 0.0005-0.0007 parts of folic acid, 0.00004-0.00006 parts of D-biotin, and 0.00006 parts of vitamin B12. 12 0.000004~0.000006 parts, 0.001~0.002 parts of anhydrous zinc acetate, 0.0005~0.0007 parts of anhydrous cobalt chloride, 0.0005~0.0007 parts of anhydrous sodium molybdate, and the balance is water. The weight parts of each component of the nutrient solution are calculated relative to the total weight parts of the eye-spotted meloidogy adult feed, and are the weight parts in 12~14 parts of the nutrient solution.

2. The feed for adults of Meloidogyne sphenanthera according to claim 1, wherein It also includes 0.7-1 parts of ascorbic acid and 0.2-0.4 parts of sorbic acid.

3. A method for preparing the feed for adults of Meloidogyne sphenanthera according to claim 1, characterized in that: The following steps are involved: S1, dissolving cholesterol in chloroform, then adding casein and cellulose powder and mixing them, and then drying to obtain a first mixed material; S2. Mixing the first mixed material, Webster's salt, sucrose, L-cysteine, choline chloride, inositol, multivitamins, ethanol solution of nutrients, linoleic acid, linolenic acid, aqueous solution of nutrients, honey, applesauce, royal jelly, and part of the water to obtain a second mixed material; mixing agar powder and the remaining water and dissolving them at 90-100° C. to obtain an agar solution; S3. Mixing the second mixed material and the agar solution to obtain the adult feed of the Meloidogyne spp.

4. The method for preparing the feed for adults of Meloidogyne ocellaris according to claim 3, wherein: In step S2, the nutrient component ethanol solution is prepared by the following steps: dissolving dl-α-tocopheryl acetate, vitamin D3 and menadione in ethanol to obtain the nutrient component ethanol solution.

5. The method for preparing the feed for adults of Meloidogyne ocellaris according to claim 3, wherein: In step S2, the nutrient solution is prepared by the following steps: nicotinamide, D-calcium pantothenate, thiamine, riboflavin, pyridoxine, folic acid, D-biotin, vitamin B 12 , anhydrous zinc acetate, anhydrous cobalt chloride and anhydrous sodium molybdate are dissolved in water to obtain the nutrient solution.

6. The method for preparing feed for adults of Meloidogyne ocellaris according to claim 3, characterized in that: In step S2, the amount of water added when preparing the second mixed feed is 15% to 40% of the total weight of the water component added to the feed.

7. The method for preparing the feed for adults of Meloidogyne ocellaris according to claim 3, wherein: In step S2, the nutrient component ethanol solution or the nutrient component aqueous solution is stored at 3-5°C for later use.

8. A method for preparing the feed for adults of Meloidogyne ocellaris according to claim 2, characterized in that: The following steps are involved: S1, dissolving cholesterol in chloroform, then adding casein and cellulose powder and mixing them, and then drying to obtain a first mixed material; S2. Add ascorbic acid and sorbic acid to the first mixed material, Webster's salt, sucrose, L-cysteine, choline chloride, inositol, multivitamins, ethanol solution of nutrients, linoleic acid, linolenic acid, aqueous solution of nutrients, honey, applesauce, royal jelly, and part of the water to obtain a second mixed material; mix the agar powder and the remaining water and dissolve them at 90-100° C. to obtain an agar solution; S3. Mixing the second mixed material and the agar solution to obtain the adult feed of the Meloidogyne spp.

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