Application of traditional Chinese medicine composition in preparation of medicine for treating infectious bronchitis

Abstract

The invention discloses application of a traditional Chinese medicine composition in preparation of a medicine for treating infectious bronchitis, and belongs to the technical field of medicine. The traditional Chinese medicine composition is prepared from the following components: 6-35 parts of herba ephedrae, 10-30 parts of radix platycodonis, 15-40 parts of rhizoma phragmitis, 5-30 parts of semen armeniacae amarae, 6-60 parts of liquorice root, 12-25 parts of herba plantaginis, 5-30 parts of fructus aurantii, 75-200 parts of gypsum, 1-18 parts of radix stemonae and 2-30 parts of herba menthae. Experiments prove that the traditional Chinese medicine composition disclosed by the invention has an excellent treatment effect on avian infectious bronchitis, the cure rate of diseased chickens is remarkably improved, and the replication of viruses in chicken bodies is effectively restrained, so that the virus load is greatly reduced. Particularly, it is worth mentioning that the medicine can remarkably improve the egg laying performance of diseased laying hens, and the egg yield and the egg quality are remarkably improved.

Description

[0001] This is a divisional application. The invention name of the original application is: A drug for preventing and treating infectious bronchitis in chickens and its preparation method. The application date of the original application is: 2024-08-05, and the application number of the original application is: CN 202411060310.2. Technical Field

[0002] The present invention belongs to the field of medical technology, and particularly relates to the application of a traditional Chinese medicine composition in the preparation of a drug for treating infectious bronchitis in chickens. Background Art

[0003] Infectious Bronchitis (IB) in chickens is an acute and highly contagious respiratory disease caused by a coronavirus, posing a major threat to the poultry farming industry. This disease mainly affects laying hens and breeding chickens, resulting in decreased growth performance, increased mortality, reduced egg production, and lower eggshell quality, thus bringing significant economic losses to the chicken farming industry. Currently, there is still a lack of effective treatment means for infectious bronchitis in chickens.

[0004] In actual production, chicken flocks often suffer from concurrent bacterial diseases. Therefore, breeders often use antibacterial drugs to deal with them. Although the use of antibiotics can reduce the incidence of infectious bronchitis in chickens to a certain extent, they mainly target secondary bacterial infections caused by viral infections. However, with the widespread use of antibacterial drugs, especially broad-spectrum antibacterial drugs, in the breeding industry, the problem of drug resistance has become increasingly serious, posing a serious threat to public health, sustainable economic growth, and global economic stability. Therefore, the issue of safe use of drugs in livestock and poultry breeding has become particularly severe.

[0005] In traditional medicine, traditional Chinese medicines have been widely used to treat respiratory diseases, including infectious bronchitis in chickens. Common traditional Chinese medicine preparations on the market, such as Ganchang Koufuye, Qiangli Kechuanning, and Kechuanling, are used to relieve symptoms of asthma and cough. However, the existing traditional Chinese medicine preparations are not obvious in the treatment of infectious bronchitis in chickens, which may be related to the drug formula, preparation method, and administration route.

[0006] Currently, the preparation method of traditional Chinese medicine mainly uses the water decoction method, but this method has some obvious limitations. First, a large amount of active ingredients may be lost during the extraction process of the water decoction method, especially for those ingredients with low solubility in water. Second, in addition to containing active ingredients, the extract may also contain more impurities, which may affect the efficacy and safety of the drug. Finally, the high-temperature extraction process may destroy heat-sensitive active ingredients, further reducing the efficacy of the drug.

[0007] In order to overcome the deficiencies of the prior art, the present invention aims to provide an improved Chinese medicine preparation and a preparation method thereof to improve the therapeutic effect on infectious bronchitis in chickens. The present invention aims to reduce the loss of active ingredients, improve the purity and efficacy of the drug, and reduce the risk of drug resistance by optimizing the formula, improving the preparation process, and exploring new routes of administration, thereby providing a safer and more effective treatment plan for the poultry industry. Summary of the invention

[0008] The purpose of the present invention is to provide a medicine for preventing and treating infectious bronchitis in chickens and a preparation method thereof, thereby providing a new therapeutic medicine for treating infectious bronchitis in chickens.

[0009] To achieve the above object, the present invention provides the following technical solutions:

[0010] Firstly, the present invention provides a medicine for preventing and treating infectious bronchitis in chickens. The raw materials for preparing the medicine include the following components by weight: 6-35 parts of ephedra, 10-30 parts of platycodon, 15-40 parts of reed root, 5-30 parts of bitter almond, 6-60 parts of liquorice, 12-25 parts of plantain, 5-30 parts of fructus aurantii, 75-200 parts of gypsum, 1-18 parts of stemona and 2-30 parts of mint.

[0011] Preferably, the raw materials for preparing the medicine include the following components by weight: 25 parts of ephedra, 20 parts of platycodon, 15 parts of reed root, 25 parts of bitter almond, 50 parts of licorice, 20 parts of plantain, 20 parts of fructus aurantii, 125 parts of gypsum, 5 parts of stemona and 15 parts of mint.

[0012] Secondly, the present invention provides a method for preparing a drug for preventing and treating infectious bronchitis in chickens, the method for preparing the drug comprising the following steps:

[0013] (1) Crush the following medicinal materials: 6-35 parts of ephedra, 10-30 parts of platycodon, 15-40 parts of phragmites australis, 5-30 parts of bitter almonds, 6-60 parts of liquorice, 12-25 parts of plantain, 5-30 parts of fructus aurantii, 1-18 parts of stemonae and 2-30 parts of mint, and sieve to obtain medicinal material powder;

[0014] (2) adding distilled water 10 times the mass of the medicinal powder, stirring evenly, adding a composite biological enzyme 0.3 times the mass of the medicinal powder, and performing enzymolysis at 50° C. for 2 hours;

[0015] (3) raising the temperature to 80° C. and boiling for 2 hours, then filtering to obtain extract 1;

[0016] (4) 75-200 parts of gypsum are crushed and sieved to obtain gypsum powder, distilled water in an amount of 10 times the mass of the gypsum powder is added, and the mixture is boiled for 2 hours, and filtered to obtain extract 2 and gypsum residue;

[0017] (5) adding 8 times the mass of distilled water of the gypsum residue, stirring evenly, and continuing to boil for 2 hours. After filtering, extract 3 was obtained;

[0018] (6) combining extract 1, extract 2 and extract 3, and concentrating the mixture to a density of 1.05-1.10 g / ml to obtain a concentrated solution;

[0019] (7) The concentrated solution is filtered using a 0.5 μm ceramic membrane to obtain the drug.

[0020] Preferably, the composite biological enzyme consists of 40% cellulase, 40% pectinase and 20% neutral protease.

[0021] Secondly, the present invention provides an application of a traditional Chinese medicine composition in preparing a medicine for treating infectious bronchitis in chickens. The traditional Chinese medicine composition comprises, by weight, 6-35 parts of ephedra, 10-30 parts of platycodon, 15-40 parts of reed rhizome, 5-30 parts of bitter almond, 6-60 parts of liquorice, 12-25 parts of plantain, 5-30 parts of fructus aurantii, 75-200 parts of gypsum, 1-18 parts of stemona and 2-30 parts of mint.

[0022] Preferably, the Chinese medicine composition comprises, by weight: 25 parts of ephedra, 20 parts of platycodon, 15 parts of reed root, 25 parts of bitter almond, 50 parts of licorice, 20 parts of plantain, 20 parts of fructus aurantii, 125 parts of gypsum, 5 parts of stemona and 15 parts of mint.

[0023] Preferably, based on weight, the drug is prepared by the above-mentioned drug preparation method.

[0024] Secondly, the present invention provides an application of a traditional Chinese medicine composition in the preparation of an avian infectious bronchitis virus inhibitor. The traditional Chinese medicine composition comprises, by weight, 6-35 parts of ephedra, 10-30 parts of platycodon, 15-40 parts of reed rhizome, 5-30 parts of bitter almond, 6-60 parts of licorice, 12-25 parts of plantain, 5-30 parts of fructus aurantii, 75-200 parts of gypsum, 1-18 parts of stemona and 2-30 parts of mint.

[0025] Preferably, the Chinese medicine composition comprises, by weight: 25 parts of ephedra, 20 parts of platycodon, 15 parts of reed root, 25 parts of bitter almond, 50 parts of licorice, 20 parts of plantain, 20 parts of fructus aurantii, 125 parts of gypsum, 5 parts of stemona and 15 parts of mint.

[0026] Preferably, the drug is prepared by the above-mentioned drug preparation method.

[0027] In addition, by weight parts, the present invention provides an application of a traditional Chinese medicine composition in preparing a drug for improving the egg production and egg quality of laying hens. The traditional Chinese medicine composition includes: 6 - 35 parts of Ephedrae Herba, 10 - 30 parts of Platycodonis Radix, 15 - 40 parts of Phragmitis Rhizoma, 5 - 30 parts of Armeniacae Semen Amarum, 6 - 60 parts of Glycyrrhizae Radix et Rhizoma, 12 - 25 parts of Plantaginis Herba, 5 - 30 parts of Aurantii Fructus Immaturus, 75 - 200 parts of Gypsum Fibrosum, 1 - 18 parts of Stemonae Radix, and 2 - 30 parts of Menthae Herba.

[0028] Preferably, by weight parts, the traditional Chinese medicine composition includes: 25 parts of Ephedrae Herba, 20 parts of Platycodonis Radix, 15 parts of Phragmitis Rhizoma, 25 parts of Armeniacae Semen Amarum, 50 parts of Glycyrrhizae Radix et Rhizoma, 20 parts of Plantaginis Herba, 20 parts of Aurantii Fructus Immaturus, 125 parts of Gypsum Fibrosum, 5 parts of Stemonae Radix, and 15 parts of Menthae Herba.

[0029] Preferably, the drug is prepared by the preparation method of the above - mentioned drug.

[0030] The performance explanations of the traditional Chinese medicine components used in the present invention are as follows:

[0031] Ephedrae Herba:

[0032]

Source

[0033]

Aliases

[0034]

Original plants

[0035]

Harvesting and processing

[0036]

Medicinal properties

[0037]

Functions and indications

[0038] Glycyrrhizae Radix et Rhizoma:

[0039]

Source

[0040]

Aliases

[0041]

Original Plant

[0042] [Harvesting and processing] Dig in autumn, remove the stem base, branch forks, fibrous roots, etc., cut into sections of appropriate length, sun-dried until half-dried, tied into small bundles, and then sun-dried until completely dry. Some also have the cork bark removed, which is called "powder grass". Place in a dry and ventilated place to prevent mildew and moth.

[0043]

Properties

[0044]

Functions and Indications

[0045] Bitter almonds:

[0046] [Source] The dried mature seeds of the Rosaceae plants wild apricot, Siberian apricot, Northeast apricot or apricot.

[0047] [Synonyms] Apricot kernel (Ben Jing), apricot seed (Treatise on Febrile Diseases), mulberry seed (Shiyao Erya), bitter almond (Clinical Guide), apricot kernel (Zhejiang Handbook of Traditional Chinese Medicine).

[0048] [Original plant] Prunus armeniaca, Siberian apricot, Northeastern apricot or apricot

[0049] [Harvesting and processing] Harvest the ripe fruits in summer, remove the pulp and shell, take out the seeds, and dry them in the sun.

[0050]

Properties

[0051] [Functions and indications] It can reduce qi, relieve cough and asthma, and moisten the intestines and promote bowel movements. It is mainly used to treat cough, asthma, dry intestines and constipation.

[0052] plaster:

[0053]

Source

[0054] [Synonyms] Fine stone, fine-grained stone (Bielu), soft gypsum (Bencao Yanyi Buyi), cold water stone (Compendium of Materia Medica), white tiger (Yaoyao Huayi).

[0055]

Original Plant

[0056] [Harvesting and processing] It is usually dug in winter. After digging, the soil and rocks are removed.

[0057] [Medicinal properties] Sweet, spicy, very cold, enters the lung and stomach meridians

[0058]

Indications

[0059] Platycodon grandiflorum:

[0060]

Source

[0061]

Synonyms

[0062]

Original Plant

[0063]

Harvesting and Processing

[0064]

Properties

[0065]

Indications

[0066] Phragmites australis (Cav.) Trin. ex Steud.:

[0067]

Source

[0068]

Synonyms

[0069]

Original Plant

[0070]

Harvesting and Processing

[0071]

Properties

[0072]

Indications

[0073] Citrus aurantium L. var. amara Engl.:

[0074]

Source

[0075]

Synonyms

[0076]

Original Plant

[0077] [Harvesting and processing] Harvest in July when the peel is still green, cut into two halves horizontally from the middle, and dry in the sun or at low temperature.

[0078]

Properties

[0079] [Function and indication] Regulates qi and relieves fullness, relieves stagnation and relieves bloating. Used for qi stagnation in the chest and flanks, bloating and pain, indigestion, phlegm and fluid retention, and sagging of internal organs.

[0080] Plantain:

[0081]

Source

[0082] [Synonyms] Plantain, wheel grass, pig ear grass, cow ear grass

[0083]

Original Plant

[0084] [Harvesting and processing] Harvest in the autumn of the second year after sowing. Dig up the whole plant, wash off the mud and sand, and dry it in the sun or use it fresh.

[0085]

Properties

[0086]

Functions and indications

[0087] Hundreds:

[0088] [Source] It is the tuberous root of the Stemonaceae plants such as creeping Stemona, erect Stemona or opposite leaf Stemona. [Synonyms] cough medicine (Tao Hongjing), hundred roots, wild asparagus, hundred milk (Yang's Experience Prescriptions), nine clusters of roots (Cao Mu Bian Fang), nine insect roots (Classification of Herbal Medicines), a nest of tigers (Jiangsu Plant Medicine Records), ninety-nine roots (Chinese Native Pesticide Records), mountain hundred roots (Chinese Medicinal Records), cattle lice ghost (Mindong Materia Medica).

[0089] [Original plant] Stemonaceae plants, creeping Stemona, erect Stemona or opposite leaf Stemona

[0090] [Harvesting and processing] Dig up the seeds before new buds emerge in spring and when the seedlings are about to wither in autumn, clean the soil, remove the fibrous roots, blanch them in boiling water, and then take them out and dry them in the sun.

[0091]

Properties

[0092]

Functions and Indications

[0093] Mint:

[0094]

Source

[0095]

Synonyms

[0096]

Original Plant

[0097]

Harvesting and Processing

[0098]

Medicinal Properties

[0099]

Functions and Indications

[0100] The beneficial effects of the present invention are as follows:

[0101] The present invention provides a traditional Chinese medicine composition for preventing and treating infectious bronchitis in chickens, which has remarkable therapeutic effects and safety. Experimental data show that this composition can effectively improve the cure rate of diseased chickens. At the same time, the present invention significantly reduces the viral load in diseased chickens, demonstrating a strong antiviral ability and effectively controlling the spread and development of the disease.

[0102] In addition, the present invention performs excellently in improving the production performance of diseased laying hens. Experimental results show that after using this traditional Chinese medicine composition, the average egg production and eggshell thickness of laying hens are significantly increased, and the number of deformed eggs is greatly reduced. This not only improves the egg quality but also effectively reduces the economic losses of the farm, showing significant economic benefits. At the same time, as a pure traditional Chinese medicine preparation, no obvious adverse reactions are observed during the treatment process of the present invention, demonstrating good safety.

[0103] Through the synergistic effect of various medicinal materials, the traditional Chinese medicine composition of the present invention achieves multi-target and multi-pathway therapeutic effects, reflecting the holistic concept and syndrome differentiation and treatment characteristics of traditional Chinese medicine. It not only provides an effective non-antibiotic alternative for infectious bronchitis in chickens, helps reduce the use of antibiotics in the breeding industry, but also may have potential therapeutic effects on other similar avian respiratory diseases. Combined with its simple and feasible preparation method, the present invention has important practical application value and broad market prospects. Description of the Drawings

[0104] Figure 1 The state diagram of the trachea of the chickens in Group 4 of Example 3 days after virus challenge;

[0105] Figure 2 The state diagram of the trachea of the chickens in Group 4 of Example 5 days after treatment;

[0106] Figure 3 The state diagram of the trachea of the chickens in Group 7 of Example 3 days after virus challenge;

[0107] Figure 4 The state diagram of the trachea of the chickens in Group 7 of Example 5 days after treatment;

[0108] Figure 5 The state diagram of the trachea of the chickens in Group 4 of Comparative Example 3 days after virus challenge;

[0109] Figure 6 The state diagram of the trachea of the chickens in Group 4 of Comparative Example 5 days after treatment. Detailed implementation mode

[0110] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0111] Example 1

[0112] A drug 1 for preventing and treating chicken infectious bronchitis

[0113] This drug 1 is composed of 15 parts of Ephedrae Herba, 10 parts of Platycodonis Radix, 15 parts of Phragmitis Rhizoma, 15 parts of Armeniacae Semen Amarum, 30 parts of Glycyrrhizae Radix et Rhizoma, 12 parts of Plantaginis Herba, 10 parts of Aurantii Fructus Immaturus, 75 parts of Gypsum Fibrosum, 3 parts of Stemonae Radix, and 6 parts of Menthae Herba.

[0114] This drug 1 is prepared by the following preparation method:

[0115] (1) After crushing Ephedrae Herba, Platycodonis Radix, Phragmitis Rhizoma, Armeniacae Semen Amarum, Glycyrrhizae Radix et Rhizoma, Plantaginis Herba, Aurantii Fructus Immaturus, Stemonae Radix, and Menthae Herba, pass through a No. 5 sieve to obtain drug powder;

[0116] (2) Add distilled water with a mass fraction 10 times that of the medicinal material powder, stir evenly, and then add a composite bio-enzyme (40% cellulase, 40% pectinase, 20% neutral protease) with a mass fraction 0.3 times that of the medicinal material powder, and enzymatically hydrolyze at 50 °C for 2 h;

[0117] (3) Heat up to 80 °C and decoct for 2 h, then filter to obtain Extract 1;

[0118] (4) After crushing Gypsum Fibrosum, pass through a No. 5 sieve, add distilled water with a mass fraction 10 times that of Gypsum Fibrosum, decoct for 2 h, and after filtration, obtain Extract 2 and the Gypsum Fibrosum residue;

[0119] (5) Add distilled water with a mass fraction 8 times that of the Gypsum Fibrosum residue, stir evenly, continue to decoct for 2 hours, and after filtration, obtain Extract 3;

[0120] (6) Combine extract 1, extract 2, and extract 3, and concentrate to a density of 1.05 - 1.10 g / ml to obtain a concentrated solution;

[0121] (7) Filter the concentrated solution through a 0.5 - micron ceramic membrane to obtain a clarified traditional Chinese medicine oral liquid.

[0122] Example 2

[0123] A drug 2 for preventing and treating infectious bronchitis in chickens

[0124] This drug 2 is composed of 30 parts of Ephedrae Herba, 15 parts of Platycodonis Radix, 30 parts of Phragmitis Rhizoma, 30 parts of Armeniacae Semen Amarum, 60 parts of Glycyrrhizae Radix et Rhizoma, 20 parts of Plantaginis Herba, 24 parts of Aurantii Fructus Immaturus, 90 parts of Gypsum Fibrosum, 1 part of Stemonae Radix, and 20 parts of Menthae Herba.

[0125] The preparation method of this drug 2 is the same as that in Example 1.

[0126] Example 3

[0127] A drug 3 for preventing and treating infectious bronchitis in chickens

[0128] This drug 3 is composed of 6 parts of Ephedrae Herba, 25 parts of Platycodonis Radix, 36 parts of Phragmitis Rhizoma, 5 parts of Armeniacae Semen Amarum, 6 parts of Glycyrrhizae Radix et Rhizoma, 25 parts of Plantaginis Herba, 5 parts of Aurantii Fructus Immaturus, 200 parts of Gypsum Fibrosum, 10 parts of Stemonae Radix, and 2 parts of Menthae Herba.

[0129] The preparation method of this drug 3 is the same as that in Example 1

[0130] Example 4

[0131] A drug 4 for preventing and treating infectious bronchitis in chickens

[0132] This drug 4 is composed of 25 parts of Ephedrae Herba, 20 parts of Platycodonis Radix, 15 parts of Phragmitis Rhizoma, 25 parts of Armeniacae Semen Amarum, 50 parts of Glycyrrhizae Radix et Rhizoma, 20 parts of Plantaginis Herba, 20 parts of Aurantii Fructus Immaturus, 125 parts of Gypsum Fibrosum, 5 parts of Stemonae Radix, and 15 parts of Menthae Herba.

[0133] The preparation method of this drug 4 is the same as that in Example 1.

[0134] Example 5

[0135] A drug 5 for preventing and treating infectious bronchitis in chickens

[0136] This drug 5 is composed of 35 parts of Ephedrae Herba, 20 parts of Platycodonis Radix, 25 parts of Phragmitis Rhizoma, 15 parts of Armeniacae Semen Amarum, 10 parts of Glycyrrhizae Radix et Rhizoma, 20 parts of Plantaginis Herba, 25 parts of Aurantii Fructus Immaturus, 150 parts of Gypsum Fibrosum, 18 parts of Stemonae Radix, and 2 parts of Menthae Herba.

[0137] The preparation method of this drug 5 is the same as that in Example 1.

[0138] Example 6

[0139] A drug for preventing and treating infectious bronchitis in chickens 6

[0140] This drug 6 is composed of 10 parts of Ephedrae Herba, 25 parts of Platycodonis Radix, 30 parts of Phragmitis Rhizoma, 10 parts of Armeniacae Semen Amarum, 30 parts of Glycyrrhizae Radix et Rhizoma, 18 parts of Plantaginis Herba, 30 parts of Aurantii Fructus Immaturus, 160 parts of Gypsum Fibrosum, 5 parts of Stemonae Radix, and 2 parts of Menthae Herba.

[0141] The preparation method of this drug 6 is the same as that of Example 1.

[0142] Example 7

[0143] A drug for preventing and treating infectious bronchitis in chickens 7

[0144] This drug 7 is composed of 20 parts of Ephedrae Herba, 25 parts of Platycodonis Radix, 20 parts of Phragmitis Rhizoma, 20 parts of Armeniacae Semen Amarum, 40 parts of Glycyrrhizae Radix et Rhizoma, 25 parts of Plantaginis Herba, 15 parts of Aurantii Fructus Immaturus, 120 parts of Gypsum Fibrosum, 15 parts of Stemonae Radix, and 20 parts of Menthae Herba.

[0145] The preparation method of this drug 7 is the same as that of Example 1.

[0146] Example 8

[0147] A drug for preventing and treating infectious bronchitis in chickens 8

[0148] Example 8 is composed of 10 parts of Ephedrae Herba, 30 parts of Platycodonis Radix, 40 parts of Phragmitis Rhizoma, 5 parts of Armeniacae Semen Amarum, 20 parts of Glycyrrhizae Radix et Rhizoma, 20 parts of Plantaginis Herba, 30 parts of Aurantii Fructus Immaturus, 130 parts of Gypsum Fibrosum, 5 parts of Stemonae Radix, and 30 parts of Menthae Herba.

[0149] The preparation method of this drug 8 is the same as that of Example 1.

[0150] Control Example 1:

[0151] A drug for preventing and treating infectious bronchitis in chickens 9

[0152] This drug 9 is composed of 15 parts of Ephedrae Herba Praeparata, 10 parts of Scutellariae Radix, 15 parts of Phragmitis Rhizoma, 15 parts of Armeniacae Semen Amarum, 30 parts of Glycyrrhizae Radix et Rhizoma, 12 parts of Plantaginis Herba, 10 parts of Aurantii Fructus Immaturus, 75 parts of Gypsum Fibrosum, 3 parts of Stemonae Radix, and 6 parts of Menthae Herba.

[0153] The preparation method of this drug 9 is the same as that of Example 1

[0154] Control Example 2

[0155] A drug for preventing and treating infectious bronchitis in chickens 10

[0156] This drug 10 is composed of 30 parts of Ephedrae Herba, 15 parts of Scutellariae Radix, 30 parts of Phragmitis Rhizoma, 30 parts of Armeniacae Semen Amarum, 60 parts of Glycyrrhizae Radix et Rhizoma, 20 parts of Isatidis Radix, 24 parts of Aurantii Fructus Immaturus, 90 parts of Gypsum Fibrosum, 1 part of Stemonae Radix, and 20 parts of Menthae Herba.

[0157] The preparation method of this drug 10 is the same as that of Example 1.

[0158] Comparative Example 3

[0159] A drug for preventing and treating infectious bronchitis in chickens 11

[0160] This drug 11 is composed of 6 parts of fried Ephedra, 25 parts of Scutellaria baicalensis, 36 parts of Reed Rhizome, 5 parts of Bitter Apricot Kernel, 6 parts of Licorice Root, 25 parts of Isatis Root, 5 parts of Fructus Aurantii, 200 parts of Gypsum Fibrosum, 10 parts of Stemona Root, and 2 parts of Mentha haplocalyx Briq.

[0161] The preparation method of this drug 11 is the same as that of Example 1.

[0162] Comparative Example 4

[0163] A drug for preventing and treating infectious bronchitis in chickens 12

[0164] This drug 12 is composed of 30 parts of Platycodon grandiflorum, 30 parts of Reed Rhizome, 25 parts of Bitter Apricot Kernel, 50 parts of Licorice Root, 20 parts of Plantago asiatica L., 20 parts of Fructus Aurantii, 125 parts of Gypsum Fibrosum, 5 parts of Stemona Root, and 15 parts of Mentha haplocalyx Briq.

[0165] The preparation method of this drug 12 is the same as that of Example 1.

[0166] Comparative Example 5

[0167] A drug for preventing and treating infectious bronchitis in chickens 13

[0168] This drug 13 is composed of 35 parts of Ephedra, 25 parts of Reed Rhizome, 35 parts of Bitter Apricot Kernel, 20 parts of Licorice Root, 35 parts of Fructus Aurantii, 150 parts of Gypsum Fibrosum, 18 parts of Stemona Root, and 2 parts of Mentha haplocalyx Briq.

[0169] The preparation method of this drug 13 is the same as that of Example 1.

[0170] Comparative Example 6

[0171] A drug for preventing and treating infectious bronchitis in chickens 14

[0172] This drug 14 is composed of 10 parts of Ephedra, 30 parts of Platycodon grandiflorum, 30 parts of Reed Rhizome, 10 parts of Bitter Apricot Kernel, 30 parts of Licorice Root, 20 parts of Plantago asiatica L., 30 parts of Fructus Aurantii, and 160 parts of Gypsum Fibrosum.

[0173] The preparation method of this drug 14 is the same as that of Example 1.

[0174] Comparative Example 7

[0175] A drug for preventing and treating infectious bronchitis in chickens 15

[0176] This drug 7 is composed of 40 parts of Ephedra, 45 parts of Platycodon grandiflorum, 40 parts of Bitter Apricot Kernel, 40 parts of Plantago asiatica L., 120 parts of Gypsum Fibrosum, 15 parts of Stemona Root, and 20 parts of Mentha haplocalyx Briq.

[0177] The preparation method of this drug 15 is the same as that of Example 1.

[0178] Experimental Example 1

[0179] Detect the viral load and treatment effect after treatment with different drugs

[0180] (1) At 9 days old, all chickens were weighed and randomly divided into groups: blank control group (10 chickens) and model group (160 chickens).

[0181] (2) Chickens in the blank control group were continuously raised in a negative pressure isolator, while chickens in the model group were moved to a room with controllable environmental temperature and humidity, completely disinfected, and relatively enclosed for 3 days of adaptive feeding.

[0182] (3) At 12 days old, chickens in the model group were inoculated with IBV M41 strain by eye dropping (100 μL), nasal dropping (100 μL), and the blank group was given an equal amount of sterile PBS in the same way. Two chickens in each group were dissected three days after virus challenge to observe the tracheal status of the chickens.

[0183] (4) The diseased chickens after successful modeling were randomly divided into 16 groups, with 10 chickens in each group, namely positive control group, Example 8 group, and Comparative Example 7 group.

[0184] (5) The positive control group was fed with doxycycline hydrochloride at a ratio of 1 g to 500 ml of water. Water was cut off at 3:00 pm every day, and the chickens were given the drug by concentrated drinking water for 4 hours at 5:00 pm for 5 consecutive days.

[0185] (6) The Example group and the Comparative Example group were fed with the drug of the present invention at a ratio of 1 ml to 500 ml of water. Water was cut off at 3:00 pm every day, and the chickens were given the drug by concentrated drinking water for 4 hours at 5:00 pm for 5 consecutive days.

[0186] (6) During the experiment, the disease development of the experimental chickens in each group was recorded. The mental state, appetite, and breathing condition of the chickens were observed at 9:00 am and 4:00 pm every day respectively.

[0187] (7) The dead chickens were dissected. Those showing typical lesion characteristics were judged to have died from this disease, and the number of dead chickens and the mortality rate during the entire experimental period were counted.

[0188] (8) After the treatment, the experimental chickens in each group were sacrificed to observe the organ lesions. Tracheal and kidney tissues were taken, and the viral loads in the kidneys and tracheas of each group were detected by fluorescence quantitative PCR. The experimental results were determined by comparing the viral loads in the kidneys and organs. At the same time, the number of cured, effective, and ineffective cases was counted, and the cure rate and effective rate were calculated.

[0189] The diagnostic criteria for the curative effect are as follows:

[0190] Cured: The symptoms of infectious bronchitis such as dyspnea, gasping with open mouth, coughing, and sneezing disappear.

[0191] Effective: The symptoms of infectious bronchitis such as dyspnea, gasping for breath with open mouth, coughing, and sneezing are greatly alleviated.

[0192] Ineffective: The symptoms of infectious bronchitis are not effectively alleviated.

[0193] Figures 1 - 6 Anatomical example diagrams selected for the present invention to characterize cure, effectiveness, and ineffectiveness.

[0194] Figure 1 , Figure 3 and Figure 5 are the results 3 days after virus challenge of the chickens in Example 4 group, Example 7 group, and Comparative Example 7 group respectively. It can be seen that there is bleeding in the trachea and phlegm after virus challenge. At the same time, it is observed that the chickens in the flock have obvious coughing, snoring, and wheezing sounds.

[0195] Figure 2 is the result of treating the chickens in Example 4 group for 5 days. It can be seen that the bleeding in the trachea has recovered, there is no phlegm in the trachea, and it has returned to normal after treatment. At the same time, it is observed that the symptoms such as dyspnea, gasping for breath with open mouth, coughing, and sneezing in the chicken flock have disappeared.

[0196] Figure 4 is the result of treating the chickens in Example 7 group for 5 days. It can be seen that the bleeding in the trachea has recovered, there is basically no phlegm in the trachea, and it has basically returned to normal after treatment. At the same time, it is observed that the symptoms such as dyspnea, gasping for breath with open mouth, coughing, and sneezing in the chicken flock have basically recovered.

[0197] Figure 6 is the result of treating the chickens in Comparative Example 7 group for 5 days. It can be seen that the bleeding in the trachea has not been treated and there is still phlegm in the trachea after treatment. At the same time, it is observed that some chickens still have symptoms such as dyspnea, gasping for breath with open mouth, coughing, and sneezing.

[0198] The results obtained by statistics are shown in Table 1 below.

[0199] Table 1 Detection of treatment effects after different drug treatments

[0200]

[0201]

[0202]

[0203] As can be seen from Table 1, when ephedra is replaced by honey-fried ephedra (Comparative Example 1 vs Example 1), Comparative Example 1: cure rate 40%, total effective rate 50%; while Example 1: cure rate 70%, total effective rate 100%; Conclusion: Raw ephedra is more effective than honey-fried ephedra, probably because raw ephedra retains more active ingredients.

[0204] When Platycodon grandiflorum was replaced with Scutellaria baicalensis (Comparative Example 2 vs. Example 2), Comparative Example 2: cure rate 60%, total effective rate 70%; while Example 2: cure rate 80%, total effective rate 100%; Conclusion: Platycodon grandiflorum is more suitable for this formula than Scutellaria baicalensis, probably because the effect of Platycodon grandiflorum in dispersing lung qi and resolving phlegm is more in line with the disease characteristics.

[0205] When Plantago asiatica was replaced with Isatis indigotica Fort. (Comparative Example 3 vs. Example 3), Comparative Example 3: cure rate 50%, total effective rate 60%; Example 3: cure rate 60%, total effective rate 90%. Conclusion: Plantago asiatica is more effective than Isatis indigotica Fort., probably because the effect of Plantago asiatica in resolving phlegm and relieving cough is more direct.

[0206] When Ephedra sinica Stapf was removed (Comparative Example 4 vs. Example 4), Comparative Example 4: cure rate 50%, total effective rate 40%; Example 4: cure rate 100%, total effective rate 100%; Conclusion: Ephedra sinica Stapf is an indispensable ingredient in the formula, and its effects of inducing sweating and relieving exterior syndrome, dispersing lung qi and relieving asthma are crucial.

[0207] When Platycodon grandiflorum and Plantago asiatica were removed (Comparative Example 5 vs. Example 5), Comparative Example 5: cure rate 40%, total effective rate 70%; Example 5: cure rate 60%, total effective rate 90%; Conclusion: The presence of Platycodon grandiflorum and Plantago asiatica significantly improved the treatment effect, proving their importance in the formula.

[0208] When Stemona japonica (Thunb.) Miq. and Mentha haplocalyx Briq. were removed (Comparative Example 6 vs. Example 6), Comparative Example 6: cure rate 30%, total effective rate 50%; Example 6: cure rate 70%, total effective rate 90%; Conclusion: Although the dosages of Stemona japonica (Thunb.) Miq. and Mentha haplocalyx Briq. are small, they have a significant impact on the overall effect, probably by enhancing the effects of other drugs.

[0209] When Phragmites communis Trin., Glycyrrhiza uralensis Fisch., and Aurantii Fructus Immaturus were removed (Comparative Example 7 vs. Example 7), Comparative Example 7: cure rate 20%, total effective rate 20%; Example 7: cure rate 80%, total effective rate 100%. Conclusion: Each ingredient in the formula has its unique role and together constitutes a complete treatment system.

[0210] At the same time, it can be seen from the differences between different examples that when the ingredients are the same, different addition amounts of different ingredients will also lead to differences in the final treatment results, and the treatment effect of Example 4 is significantly better than that of other examples.

[0211] Therefore, we can draw the following conclusions:

[0212] 1. Replacement or removal of any ingredient will lead to a significant decline in the treatment effect. For example, replacing Ephedra sinica Stapf with honey-fried Ephedra (Comparative Example 1) or completely removing Ephedra sinica Stapf (Comparative Example 4) significantly reduced the cure rate and total effective rate. This shows that each ingredient in the formula plays an indispensable role in the overall effect.

[0213] 2. The formula contains drugs with different functions such as diaphoresis and exterior-relieving (e.g., Ephedra), resolving phlegm and relieving cough (e.g., Platycodon grandiflorum, Plantago asiatica), clearing heat and detoxifying (e.g., Gypsum). These drugs work together to comprehensively solve various symptoms of infectious bronchitis in chickens, reflecting the overall treatment concept of traditional Chinese medicine.

[0214] 3. The dosage ratios of the components in the examples are optimized to form a balanced whole. Any change will affect the treatment effect, as shown by the results of Comparative Example 5 and Comparative Example 6. This shows that the synergistic effect depends on precise proportioning.

[0215] 4. Even the removal of components with relatively small dosages (e.g., Stemona japonica, Mentha haplocalyx) will significantly affect the effect (see Comparative Example 6). This indicates that these seemingly "insignificant" components actually play a key synergistic role in the overall formula.

[0216] 5. Although some single components (e.g., Ephedra) may have side effects when used in large doses, no obvious adverse reactions are seen in the overall formula. This may be because there is a synergistic effect among the drugs that restricts each other and reduces toxicity.

[0217] 6. When some components in the formula are replaced with other seemingly similar drugs (e.g., Scutellaria baicalensis Georgi replaces Platycodon grandiflorum, or Isatis indigotica Fort. replaces Plantago asiatica), the treatment effect will decline. This further proves the unique synergistic relationship among the components in the original formula.

[0218] Table 2 Viral loads in the kidneys and organs

[0219] Average viral load in the kidney Average viral load in the trachea Positive control 0.85*10^6 0.43*10^7 Blank control 0 0 Example 1 0.69*10^6 0.65*10^6 Example 2 0.48*10^6 0.45*10^6 Example 3 0.64*10^6 0.63*10^6 Example 4 0.24*10^6 0.12*10^6 Example 5 0.62*10^6 0.62*10^6 Example 6 0.64*10^6 0.61*10^6 Example 7 0.51*10^6 0.55*10^6 Example 8 0.61*10^6 0.59*10^6 Comparative Example 1 1.25*10^8 0.58*10^8 Comparative Example 2 3.31*10^6 1.99*10^6 Comparative Example 3 1.75*10^7 1.07*10^7 Comparative Example 4 3.65*10^7 1.19*10^7 Comparative Example 5 4.14*10^7 2.09*10^7 Comparative Example 6 1.10*10^8 0.57*10^8 Comparative Example 7 1.39*10^8 0.98*10^8

[0220] From the results in Table 2, we can draw the following conclusions:

[0221] 1. The viral load data is highly consistent with the previous symptom improvement data, confirming the effectiveness of the example formula from two different perspectives. Moreover, Example 4 not only shows the best performance in symptom improvement (100% cure rate), but also shows the strongest effect in virus clearance. Therefore, the formula combination of Example 4 has unexpected technical effects.

[0222] 2. All the example formulas show stronger antiviral effects than the comparative examples and the positive control (Doxycycline Hydrochloride). This indicates that the traditional Chinese medicine formula of the present invention can not only relieve symptoms, but also directly affect the replication and transmission of the virus.

[0223] 3. The viral load data further supports the existence of a strong synergistic effect among the components in the formula. The replacement or removal of any component leads to a significant increase in the viral load, which is consistent with the previously observed symptom improvement effect.

[0224] Experimental Example 2

[0225] Since infectious bronchitis can significantly affect the egg - laying ability and eggshell quality of laying hens

[0226] (1) Select a chicken house infected with infectious bronchitis and randomly select 800 diseased chickens. Randomly divide 800 120 - day - old laying hens with infectious bronchitis into 16 groups, with 50 hens in each group: a positive control group, Example 1 - 8 groups, and Example 1 - 7 groups; at the same time, set 120 - day - old laying hens as a healthy control group and isolate them by group.

[0227] Among them, in the Example 1 - 8 groups, the oral liquid prepared in Example 1 - 8 is fed at 5 ml diluted in 1000 ml of water, and the positive control group is fed with 5 g of doxycycline hydrochloride diluted in 1000 ml of water; drink water centrally for 4 hours.

[0228] At the same time, the healthy control group is fed with an equal amount of normal saline;

[0229] (2) Feed freely for 10 consecutive days. Record the number of eggs laid every day. After 10 days, count the average number of eggs laid, the average eggshell thickness, and the average number of abnormal eggs per day. The results obtained are shown in Table 3.

[0230] Table 3 Differences in average egg - laying of different treatment groups

[0231]

[0232] From the results in Table 3, it can be seen that the egg - laying amounts of all examples of the present invention are higher than those of the comparative example and the positive control group. Among them, the performance of Example 4 is not only significantly higher than that of the comparative example and the positive control group, but also significantly better than other examples, showing unexpected technical effects.

[0233] At the same time, the eggshell thickness and the number of abnormal eggs in all examples are better than those of the comparative example and the positive control group. Similarly, the effect of Example 4 is the best, and the eggshell thickness and the number of abnormal eggs are close to those of the healthy control group.

[0234] Therefore, we can draw the following conclusions:

[0235] 1. The formula of the present invention, especially Example 4, can effectively improve the egg - laying amount, eggshell quality, and the number of abnormal eggs of chickens infected with infectious bronchitis. This means that the formula can effectively restore the egg - laying ability of infected chickens and greatly reduce the economic losses caused by the disease. Secondly, the improvement of eggshell quality not only increases the market value of eggs but also reduces the breakage rate of eggs during transportation and storage.

[0236] 2. This formula can not only effectively eliminate viruses and relieve symptoms, but also comprehensively improve the production performance of chickens. This multi-faceted improvement effect reflects the advantages of TCM holistic treatment and also illustrates the unique value of the compound in treating infectious bronchitis in chickens.

[0237] 3. The synergistic effect of the ingredients in the formula is not only reflected in antiviral and symptom improvement, but also directly affects the production performance of chickens. Whether it is replacing or missing some of the ingredients in the formula, it will significantly reduce the egg production and eggshell quality of the chickens.

[0238] 4. Example 4 again performed best in egg-laying related indicators, indicating that it has the best therapeutic effect. The possible reason is that Example 4 just achieved the optimal dose-effect relationship of various medicinal materials, so that each component works within its most effective dose range. The proportion of each medicinal material in Example 4 produced the best synergistic effect. For example, the ratio of ephedra and gypsum may have achieved a good balance between evacuating exogenous evil and clearing away heat and detoxifying, thereby achieving an effect that is far superior to other embodiments.

[0239] In general, the formula of the present invention effectively solves the problem of decreased egg production rate and decreased egg quality caused by infectious bronchitis in chickens through its powerful antiviral effect, symptom improvement ability and comprehensive recovery of production performance. It not only provides an efficient disease prevention and treatment method for the breeding industry, but also opens up a new way for the application of traditional Chinese medicine in animal husbandry. This method of combining traditional Chinese medicine theory with modern scientific research demonstrates the great potential of traditional Chinese medicine in solving modern agricultural problems.

Claims

1. Use of a Chinese medicine composition in the preparation of a drug for treating infectious bronchitis in chickens, characterized in that: The traditional Chinese medicine composition consists of the following ingredients by weight: 6-35 parts of ephedra, 10-30 parts of platycodon, 15-40 parts of reed root, 5-30 parts of bitter almond, 6-60 parts of licorice, 12-25 parts of plantain, 5-30 parts of fructus aurantii, 75-200 parts of gypsum, 1-18 parts of stemona and 2-30 parts of mint.

2. The use of a Chinese medicine composition according to claim 1 in the preparation of a drug for treating infectious bronchitis in chickens, characterized in that, by weight, The Chinese medicine composition comprises the following ingredients Composition: 25 parts of ephedra, 20 parts of platycodon, 15 parts of reed root, 25 parts of bitter almond, 50 parts of liquorice, 20 parts of plantain, 20 parts of fructus aurantii, 125 parts of gypsum, 5 parts of stemona and 15 parts of mint.

3. Use of a Chinese medicine composition in the preparation of an avian infectious bronchitis virus inhibitor, characterized in that: The traditional Chinese medicine composition consists of the following ingredients by weight: 6-35 parts of ephedra, 10-30 parts of platycodon, 15-40 parts of reed root, 5-30 parts of bitter almond, 6-60 parts of licorice, 12-25 parts of plantain, 5-30 parts of fructus aurantii, 75-200 parts of gypsum, 1-18 parts of stemona and 2-30 parts of mint.

4. Use of a traditional Chinese medicine composition according to claim 3 in the preparation of an avian infectious bronchitis virus inhibitor, characterized in that: The Chinese medicine composition comprises the following components by weight: Composition: 25 parts of ephedra, 20 parts of platycodon, 15 parts of reed root, 25 parts of bitter almond, 50 parts of liquorice, 20 parts of plantain, 20 parts of fructus aurantii, 125 parts of gypsum, 5 parts of stemona and 15 parts of mint.

5. Use of a Chinese medicine composition in the preparation of a medicament for improving egg production and egg quality of laying hens suffering from infectious bronchitis, characterized in that: The traditional Chinese medicine composition consists of the following components by weight: 6-35 parts of ephedra, 10-30 parts of platycodon, 15-40 parts of reed root, 5-30 parts of bitter almond, 6-60 parts of licorice, 12-25 parts of plantain, 5-30 parts of fructus aurantii, 75-200 parts of gypsum, 1-18 parts of stemona and 2-30 parts of mint.

6. Use of a Chinese medicine composition according to claim 5 in the preparation of a medicament for improving egg production and egg quality of laying hens suffering from infectious bronchitis, characterized in that: The traditional Chinese medicine composition is composed of the following components by weight: 25 parts of ephedra, 20 parts of platycodon, 15 parts of reed root, 25 parts of bitter almond, 50 parts of liquorice, 20 parts of plantain, 20 parts of fructus aurantii, 125 parts of gypsum, 5 parts of stemona and 15 parts of mint.

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