Composition containing blue copper peptide and application of composition in preparation of product with acne removing effect

Abstract

The invention belongs to the technical field of cosmetics, and particularly discloses a blue copper peptide-containing composition and application thereof in preparation of a product with an acne removing effect. Comprising the following components in percentage by weight: 0.05%-0.5% of p-hydroxyacetophenone, 0.05%-0.5% of a thickening agent, 0.05%-0.2% of a centella asiatica extract, 0.1%-0.5% of tocopheryl acetate, 0.1%-0.5% of dipotassium glycyrrhizinate, 0.2%-0.6% of lime peel oil, 0.2%-1% of blue copper peptide, 0.8%-1.2% of a pepper fruit extract, 0.5%-2% of butanediol, 0.5%-2% of a glycoside compound, 1%-8% of glyceryl polyether-26, 1%-10% of glycerol and the balance of water. According to the acne-removing composition, the blue copper peptide, the pepper fruit extract and the lime peel oil are combined and mutually promote under the synergistic effect, so that the acne-removing effect is effectively improved.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and particularly relates to a composition containing blue copper peptide and its application in the preparation of products with acne-removing effects. Background Art

[0002] Acne (or pimples) is a common inflammatory disease of the skin, mainly related to factors such as excessive sebum secretion from sebaceous glands, pore blockage, Propionibacterium acnes infection, and inflammatory reactions. At present, there are various acne-removing products on the market, but their effects vary, and some products may cause irritation or side effects to the skin. Therefore, it is particularly important to develop a safe, effective product with good acne-removing effects.

[0003] Blue copper peptide is a copper-containing tripeptide with significant anti-inflammatory, skin repair-promoting, antioxidant and other effects. Research has found that blue copper peptide can effectively improve skin problems through multiple effects such as promoting collagen synthesis, repairing skin damage, and reducing inflammatory reactions, and shows certain curative effects particularly in acne treatment.

[0004] In view of this, the present application is proposed. Summary of the Invention

[0005] The present invention provides a composition containing blue copper peptide and its application in the preparation of products with acne-removing effects, and the composition of the present invention has excellent acne-removing effects.

[0006] The present invention solves its technical problems by adopting the following technical solutions: A composition containing blue copper peptide, comprising the following components in weight percentages: 0.05 - 0.5% of p-hydroxyacetophenone, 0.05 - 0.5% of thickener, 0.05 - 0.2% of Centella asiatica extract, 0.1 - 0.5% of tocopheryl acetate, 0.1 - 0.5% of dipotassium glycyrrhizinate, 0.2 - 0.6% of lime peel oil, 0.2 - 1% of blue copper peptide, 0.8 - 1.2% of Zanthoxylum bungeanum fruit extract, 0.5 - 2% of butanediol, 0.5 - 2% of glycosides, 1 - 8% of glycerol polyether-26, 1 - 10% of glycerol, and the balance being water.

[0007] Blue copper peptide (inci name: tripeptide-1 copper): Blue copper peptide can effectively reduce the inflammatory reaction of the skin, relieve the redness and pain of acne, has excellent oxidation effect, has the effect of improving resistance to free radical damage, reduces the oxidative stress of the skin, improves the stability of other components, relieves acne caused by excessive sebaceous gland activity, inhibits the reproduction of Propionibacterium acnes pathogens on the skin, prevents the growth and infection of bacteria, and at the same time, its copper ion characteristics can promote the penetration and absorption of other components.

[0008] Lime peel oil has natural antibacterial and antifungal effects, can inhibit the growth of bacteria on the skin surface, especially Propionibacterium acnes, and reduce the occurrence of acne from the source. At the same time, it can regulate the sebum secretion of the skin, reduce excessive sebum secretion, avoid pore blockage and the formation of acne, and reduce the inducement of acne.

[0009] Prickly ash fruit extract: It has significant antibacterial effects, has excellent activity in inhibiting Propionibacterium acnes (the main bacteria causing acne), and at the same time helps to soothe and relieve allergies, effectively reducing skin redness and inflammatory reactions. Moreover, it can promote local blood circulation and accelerate the healing of acne.

[0010] The inventors of the present invention have found through research that the copper peptide, prickly ash fruit extract, and lime peel oil have significant synergistic effects in treating acne. The lack of any one of them or the replacement with other ingredients will lead to a significant decline in the acne treatment effect.

[0011] As a preferred embodiment of the present invention, the thickener includes at least one of xanthan gum, carbomer, hydrolyzed sclerotium gum, Caesalpinia spinosa gum, Chondrus crispus extract, and hydroxyethyl cellulose.

[0012] As a preferred embodiment of the present invention, the glycoside compound includes at least one of glycerol glucoside, cocoyl glucoside, and decyl glucoside.

[0013] As a preferred embodiment of the present invention, the preparation method of the prickly ash fruit extract includes the following steps: (1) Add prickly ash fruit powder, cellulase, and β-glucanase to water, perform the first enzymatic hydrolysis, and filter to obtain the first enzymatic hydrolysate; (2) Add ficin and alkaline protease to the first enzymatic hydrolysate, perform the second enzymatic hydrolysis to obtain the second enzymatic hydrolysate; (3) Ultrafilter the second enzymatic hydrolysate first with an ultrafiltration membrane with a molecular weight cut-off of 300 Da, then ultrafilter it with an ultrafiltration membrane with a molecular weight cut-off of 3000 Da, and dry to obtain the prickly ash fruit extract.

[0014] As a preferred embodiment of the present invention, the particle size of the prickly ash fruit powder is 20 - 200 mesh.

[0015] As a preferred embodiment of the present invention, the mass ratio of the prickly ash fruit powder, cellulase, β-glucanase, and water is 1:(0.01 - 0.02):(0.002 - 0.006):(5 - 12).

[0016] As a preferred embodiment of the present invention, the mass ratio of ficin, alkaline protease, and the first enzymatic hydrolysate is (0.005 - 0.012):(0.004 - 0.008):1.

[0017] As a preferred embodiment of the present invention, the temperature of the first enzymatic hydrolysis is 45-50 °C, and the time of the first enzymatic hydrolysis is 2-6 h; the temperature of the second enzymatic hydrolysis is 45-50 °C, and the time of the second enzymatic hydrolysis is 2-6 h.

[0018] The present invention also provides a method for preparing a composition of blue copper peptide, comprising the following steps: Add a thickening agent, p-hydroxyacetophenone, butanediol, glycoside compound, glycerol polyether-26, and glycerol to water, heat to 65-80 °C, stir evenly, cool to 40-45 °C, and add centella asiatica extract, tocopheryl acetate, dipotassium glycyrrhizinate, lime peel oil, blue copper peptide, and zanthoxylum bungeanum fruit extract, and stir evenly to obtain a composition containing blue copper peptide.

[0019] The present invention also provides an application of a composition containing blue copper peptide in the preparation of a product with acne treatment effect.

[0020] Advantages of the present invention: By combining blue copper peptide, zanthoxylum bungeanum fruit extract, and lime peel oil, and through their synergistic effect, the acne treatment effect is effectively improved. Specific embodiments

[0021] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0022] In the present invention, among the technically characterized described in an open-ended manner, a closed technical solution composed of the listed features is included, as well as an open technical solution containing the listed features.

[0023] In the present invention, regarding the numerical range, unless otherwise specified, the above numerical range is considered continuous, and includes the minimum value and the maximum value of this range, as well as each value between such minimum value and maximum value. Further, when the range refers to an integer, each integer between the minimum value and the maximum value of this range is included. In addition, when multiple ranges are provided to describe features or characteristics, these ranges can be combined. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all sub-ranges subsumed therein.

[0024] In the present invention, there is no particular limitation on the specific dispersion and stirring treatment methods.

[0025] The reagents or instruments used in the present invention without indicating the manufacturer are all conventional products that can be obtained commercially. The raw materials used in the comparative examples and the raw materials used in the parallel experiments of the embodiments are the same commercially available products unless otherwise specified.

[0026] The blue copper peptide (tripeptide-1 copper) of the present invention was purchased from JTBiotech.

[0027] Lime peel oil is sourced from Lanhai Bio.

[0028] The remaining raw materials are of conventional commercial cosmetic grade.

[0029] Example 1 A composition containing blue copper peptide comprises the following components in weight percentage: 0.2% p-hydroxyacetophenone, 0.15% xanthan gum, 0.1% Centella asiatica extract, 0.4% tocopherol acetate, 0.4% dipotassium glycyrrhizate, 0.4% lime peel oil, 0.5% blue copper peptide, 1.1% Zanthoxylum bungeanum fruit extract, 1.5% butylene glycol, 1.5% glyceryl glucoside, 4% glyceryl polyether-26, 6% glycerol, and the balance water.

[0030] The preparation method of the Zanthoxylum bungeanum fruit extract comprises the following steps: (1) Grinding the jellyfish to 200 meshes to obtain jellyfish powder; Adding fish gum fruit powder, cellulase and β-glucanase into water, performing enzymolysis at 48° C. for 4 hours, filtering and obtaining a first enzymolysis solution; The mass ratio of the fish gum fruit powder, cellulase, beta-glucanase and water is 1:0.02:0.006:12.

[0031] (2) adding fig protease and alkaline protease to the first enzymatic hydrolysis solution, performing enzymatic hydrolysis at 48° C. for 4 hours, and obtaining a second enzymatic hydrolysis solution; the mass ratio of the fig protease, alkaline protease, and the first enzymatic hydrolysis solution is 0.012:0.004:1.

[0032] (3) The second enzymatic hydrolyzate is first ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 300 Da, and then ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 3000 Da, and dried to obtain a Zanthoxylum bungeanum fruit extract.

[0033] The preparation method of the blue copper peptide composition comprises the following steps: Add thickener, p-hydroxyacetophenone, butylene glycol, glycoside compounds, glyceryl polyether-26, and glycerin to water, heat to 70°C, stir evenly, cool to 45°C, add Centella asiatica extract, tocopherol acetate, dipotassium glycyrrhizate, lime peel oil, blue copper peptide, and Zanthoxylum bungeanum fruit extract, stir evenly to obtain a composition containing blue copper peptide.

[0034] Example 2 A composition containing copper peptide, comprising the following components in percentage by weight: 0.2% of p-hydroxyacetophenone, 0.15% of xanthan gum, 0.1% of centella asiatica extract, 0.4% of tocopheryl acetate, 0.4% of dipotassium glycyrrhizinate, 0.2% of lime peel oil, 0.6% of copper peptide, 1.2% of zanthoxylum bungeanum fruit extract, 1.5% of butanediol, 1.5% of glucosylglycerol, 4% of glycerol polyether-26, 6% of glycerol, and the balance of water.

[0035] The preparation method of the zanthoxylum bungeanum fruit extract comprises the following steps: (1) Crushing the zanthoxylum bungeanum fruit to 200 meshes to obtain zanthoxylum bungeanum fruit powder; Adding the zanthoxylum bungeanum fruit powder, cellulase, and β-glucanase into water, enzymatically hydrolyzing at 48°C for 4 h, and filtering to obtain a first enzymatically hydrolyzed solution; The mass ratio of the zanthoxylum bungeanum fruit powder, cellulase, β-glucanase, and water is 1:0.02:0.006:12.

[0036] (2) Adding ficin and alkaline protease into the first enzymatically hydrolyzed solution, enzymatically hydrolyzing at 48°C for 4 h to obtain a second enzymatically hydrolyzed solution; the mass ratio of ficin, alkaline protease, and the first enzymatically hydrolyzed solution is 0.012:0.004:1.

[0037] (3) Ultrafiltering the second enzymatically hydrolyzed solution first with an ultrafiltration membrane with a molecular weight cut-off of 300 Da, then ultrafiltering with an ultrafiltration membrane with a molecular weight cut-off of 3000 Da, and drying to obtain the zanthoxylum bungeanum fruit extract.

[0038] The preparation method of the composition containing copper peptide comprises the following steps: Adding a thickening agent, p-hydroxyacetophenone, butanediol, glycoside compounds, glycerol polyether-26, and glycerol into water, heating to 70°C, stirring evenly, cooling to 45°C, adding centella asiatica extract, tocopheryl acetate, dipotassium glycyrrhizinate, lime peel oil, copper peptide, and zanthoxylum bungeanum fruit extract, and stirring evenly to obtain the composition containing copper peptide.

[0039] Example 3 A composition containing copper peptide, comprising the following components in percentage by weight: 0.2% of p-hydroxyacetophenone, 0.15% of xanthan gum, 0.1% of centella asiatica extract, 0.4% of tocopheryl acetate, 0.4% of dipotassium glycyrrhizinate, 1% of lime peel oil, 0.2% of copper peptide, 0.8% of zanthoxylum bungeanum fruit extract, 1.5% of butanediol, 1.5% of glucosylglycerol, 4% of glycerol polyether-26, 6% of glycerol, and the balance of water.

[0040] The preparation method of the zanthoxylum bungeanum fruit extract comprises the following steps: (1) Crushing the zanthoxylum bungeanum fruit to 200 meshes to obtain zanthoxylum bungeanum fruit powder; Add fish maw fruit powder, cellulase, and β-glucanase to water, enzymatically hydrolyze at 48 °C for 4 h, and filter to obtain the first enzymatically hydrolyzed solution. The mass ratio of the fish maw fruit powder, cellulase, β-glucanase, and water is 1:0.02:0.006:12.

[0041] (2) Add ficin and alkaline protease to the first enzymatically hydrolyzed solution, enzymatically hydrolyze at 48 °C for 4 h to obtain the second enzymatically hydrolyzed solution; the mass ratio of the ficin, alkaline protease, and the first enzymatically hydrolyzed solution is 0.012:0.004:1.

[0042] (3) Ultrafilter the second enzymatically hydrolyzed solution first with an ultrafiltration membrane with a molecular weight cut-off of 300 Da, and then ultrafilter with an ultrafiltration membrane with a molecular weight cut-off of 3000 Da, and dry to obtain the pepper fruit extract.

[0043] The preparation method of the said composition of copper peptide includes the following steps: Add thickener, p-hydroxyacetophenone, butanediol, glycoside compound, glycerol polyether-26, and glycerol to water, heat to 70 °C, stir evenly, cool down to 45 °C, add centella asiatica extract, tocopheryl acetate, dipotassium glycyrrhizinate, lime peel oil, copper peptide, and pepper fruit extract, and stir evenly to obtain the composition containing copper peptide.

[0044] Example 4 A composition containing copper peptide, including the following components in weight percentage: 0.2% p-hydroxyacetophenone, 0.15% xanthan gum, 0.1% centella asiatica extract, 0.4% tocopheryl acetate, 0.4% dipotassium glycyrrhizinate, 0.4% lime peel oil, 0.5% copper peptide, 1.1% pepper fruit extract, 1.5% butanediol, 1.5% glycerol glucoside, 4% glycerol polyether-26, 6% glycerol, and the balance water.

[0045] The preparation method of the said pepper fruit extract includes the following steps: (1) Crush the fish maw fruit to 200 meshes to obtain fish maw fruit powder; Add fish maw fruit powder, cellulase, and β-glucanase to water, enzymatically hydrolyze at 48 °C for 4 h, and filter to obtain the first enzymatically hydrolyzed solution. The mass ratio of the fish maw fruit powder, cellulase, β-glucanase, and water is 1:0.01:0.002:5.

[0046] (2) Add ficin and alkaline protease to the first enzymatically hydrolyzed solution, enzymatically hydrolyze at 48 °C for 4 h to obtain the second enzymatically hydrolyzed solution; the mass ratio of the ficin, alkaline protease, and the first enzymatically hydrolyzed solution is 0.005:0.008:1.

[0047] (3) Ultrafilter the second enzymatic hydrolysate first with an ultrafiltration membrane with a molecular weight cut-off of 300 Da, then ultrafilter it with an ultrafiltration membrane with a molecular weight cut-off of 3000 Da, and dry to obtain the prickly ash fruit extract.

[0048] The preparation method of the composition of the copper peptide includes the following steps: Add thickener, p-hydroxyacetophenone, butanediol, glycoside compounds, glycerol polyether-26, and glycerol to water, heat to 70 °C, stir evenly, cool down to 45 °C, add centella asiatica extract, tocopheryl acetate, dipotassium glycyrrhizinate, lime peel oil, copper peptide, and prickly ash fruit extract, and stir evenly to obtain the composition containing copper peptide.

[0049] Comparative Example 1

[0050] The difference between Comparative Example 1 and Example 1 is that Comparative Example 1 does not contain copper peptide, and the others are the same.

[0051] A composition containing copper peptide, comprising the following components in weight percentage: 0.2% p-hydroxyacetophenone, 0.15% xanthan gum, 0.1% centella asiatica extract, 0.4% tocopheryl acetate, 0.4% dipotassium glycyrrhizinate, 0.4% lime peel oil, 1.1% prickly ash fruit extract, 1.5% butanediol, 1.5% glucosylglycerol, 4% glycerol polyether-26, 6% glycerol, and the balance water.

[0052] Comparative Example 2

[0053] The difference between Comparative Example 2 and Example 1 is that Comparative Example 2 does not contain prickly ash fruit extract, and the others are the same.

[0054] A composition containing copper peptide, comprising the following components in weight percentage: 0.2% p-hydroxyacetophenone, 0.15% xanthan gum, 0.1% centella asiatica extract, 0.4% tocopheryl acetate, 0.4% dipotassium glycyrrhizinate, 0.4% lime peel oil, 0.5% copper peptide, 1.5% butanediol, 1.5% glucosylglycerol, 4% glycerol polyether-26, 6% glycerol, and the balance water.

[0055] Comparative Example 3

[0056] The difference between Comparative Example 3 and Example 1 is that Comparative Example 3 does not contain lime peel oil, and the others are the same.

[0057] A composition containing copper peptide, comprising the following components in weight percentage: 0.2% p-hydroxyacetophenone, 0.15% xanthan gum, 0.1% centella asiatica extract, 0.4% tocopheryl acetate, 0.4% dipotassium glycyrrhizinate, 0.5% copper peptide, 1.1% prickly ash fruit extract, 1.5% butanediol, 1.5% glucosylglycerol, 4% glycerol polyether-26, 6% glycerol, and the balance water.

[0058] Comparative Example 4

[0059] The difference between Comparative Example 4 and Example 1 is that Comparative Example 4 does not contain copper peptide GHK (the total amount of copper peptide GHK, prickly ash fruit extract, and lime peel oil is the same), and the others are the same.

[0060] A composition containing copper peptide GHK, comprising the following components in weight percentages: 0.2% p-hydroxyacetophenone, 0.15% xanthan gum, 0.1% asiatic pennywort extract, 0.4% tocopheryl acetate, 0.4% dipotassium glycyrrhizinate, 0.65% lime peel oil, 1.35% prickly ash fruit extract, 1.5% butanediol, 1.5% glucosylglycerol, 4% glycerol polyether-26, 6% glycerol, and the balance being water.

[0061] Comparative Example 5

[0062] The difference between Comparative Example 5 and Example 1 is that Comparative Example 5 does not contain prickly ash fruit extract (the total amount of copper peptide GHK, prickly ash fruit extract, and lime peel oil is the same), and the others are the same.

[0063] A composition containing copper peptide GHK, comprising the following components in weight percentages: 0.2% p-hydroxyacetophenone, 0.15% xanthan gum, 0.1% asiatic pennywort extract, 0.4% tocopheryl acetate, 0.4% dipotassium glycyrrhizinate, 0.95% lime peel oil, 1.05% copper peptide GHK, 1.5% butanediol, 1.5% glucosylglycerol, 4% glycerol polyether-26, 6% glycerol, and the balance being water.

[0064] Comparative Example 6

[0065] The difference between Comparative Example 6 and Example 1 is that Comparative Example 6 does not contain lime peel oil (the total amount of copper peptide GHK, prickly ash fruit extract, and lime peel oil is the same), and the others are the same.

[0066] A composition containing copper peptide GHK, comprising the following components in weight percentages: 0.2% p-hydroxyacetophenone, 0.15% xanthan gum, 0.1% asiatic pennywort extract, 0.4% tocopheryl acetate, 0.4% dipotassium glycyrrhizinate, 0.7% copper peptide GHK, 1.3% prickly ash fruit extract, 1.5% butanediol, 1.5% glucosylglycerol, 4% glycerol polyether-26, 6% glycerol, and the balance being water.

[0067] Comparative Example 7

[0068] The difference between Comparative Example 7 and Example 1 is that Comparative Example 7 uses an equal amount of sophora flavescens extract to replace the prickly ash fruit extract, and the others are the same.

[0069] A composition containing blue copper peptide comprises the following components in weight percentage: 0.2% p-hydroxyacetophenone, 0.15% xanthan gum, 0.1% Centella asiatica extract, 0.4% tocopherol acetate, 0.4% dipotassium glycyrrhizate, 0.4% lime peel oil, 0.5% blue copper peptide, 1.1% sophora flavescens extract, 1.5% butylene glycol, 1.5% glyceryl glucoside, 4% glyceryl polyether-26, 6% glycerol, and the balance water.

[0070] Comparative Example 8

[0071] The difference between Comparative Example 8 and Example 1 is that Comparative Example 8 uses an equal amount of geranium oil to replace the lime peel oil, and the other contents are the same.

[0072] A composition containing blue copper peptide comprises the following components in percentage by weight: 0.2% p-hydroxyacetophenone, 0.15% xanthan gum, 0.1% Centella asiatica extract, 0.4% tocopherol acetate, 0.4% dipotassium glycyrrhizate, 0.4% geranium oil, 0.5% blue copper peptide, 1.1% Zanthoxylum fruit extract, 1.5% butylene glycol, 1.5% glyceryl glucoside, 4% glyceryl polyether-26, 6% glycerol, and the balance water.

[0073] Comparative Example 9

[0074] The difference between Comparative Example 9 and Example 1 is that the preparation method of the fish gum fruit extract in Comparative Example 9 is different from that in Example 1, and the rest are the same.

[0075] The preparation method of the Zanthoxylum bungeanum fruit extract comprises the following steps: (1) Grinding the jellyfish to 200 meshes to obtain jellyfish powder; Adding fish gum powder, cellulase and pectinase into water, performing enzymolysis at 48°C for 4 hours, filtering and obtaining enzymolysis solution; The mass ratio of the fish gum fruit powder, cellulase, pectinase and water is 1:0.02:0.006:12.

[0076] (2) The enzymatic hydrolyzate is first ultrafiltered through an ultrafiltration membrane with a molecular weight cutoff of 300 Da, and then ultrafiltered through an ultrafiltration membrane with a molecular weight cutoff of 3000 Da, and dried to obtain a Zanthoxylum bungeanum fruit extract.

[0077] Comparative Example 10

[0078] The difference between Comparative Example 10 and Example 1 is that the preparation method of the fish gum fruit extract in Comparative Example 10 is different from that in Example 1, and the others are the same.

[0079] The preparation method of the Zanthoxylum bungeanum fruit extract comprises the following steps: (1) Grinding the jellyfish to 200 meshes to obtain jellyfish powder; Add fish maw fruit powder, cellulase, and β-glucanase to water, enzymatically hydrolyze at 48 °C for 4 h, and filter to obtain the first enzymatic hydrolysate. The mass ratio of the fish maw fruit powder, cellulase, β-glucanase, and water is 1:0.02:0.006:12.

[0080] (2) Add ficin and alkaline protease to the first enzymatic hydrolysate, enzymatically hydrolyze at 48 °C for 4 h to obtain the second enzymatic hydrolysate; the mass ratio of the ficin, alkaline protease, and the first enzymatic hydrolysate is 0.012:0.004:1.

[0081] (3) Ultrafilter the second enzymatic hydrolysate first with an ultrafiltration membrane with a molecular weight cut-off of 10,000 Da, and then ultrafilter with an ultrafiltration membrane with a molecular weight cut-off of 50,000 Da, and dry to obtain the fish maw fruit extract.

[0082] Test Example

[0083] 1. Propionibacterium acnes inhibition rate: Refer to Appendix C4 "Test Method for Bacteriostatic Performance of Dissolving Antibacterial (Inhibitory) Products" in GB15979-2002 "Hygiene Standard for Disposable Sanitary Products" to measure the bacteriostatic performance of the examples and comparative examples of the present invention. The test results are shown in Table 1.

[0084] (1) Preparation of bacterial suspension: Take the 24-hour culture of Propionibacterium acnes and wash it off with 0.03 mol / L phosphate buffer to prepare a 10 5 CFU / mL bacterial suspension.

[0085] (2) Take 0.5 g of the test composition respectively, add 0.1 mL of the above bacterial suspension, coat and mix evenly, start timing, add 9 mL of 0.03 mol / L phosphate buffer after 20 min, mix well, make appropriate dilutions, take 3 dilutions among them, take 1 mL respectively and place them in two parallel petri dishes, then add the corresponding culture medium and culture under anaerobic conditions at 36 °C for 24 hours, and perform colony counting. The above test is repeated 3 times, take the average value, and set a blank control group.

[0086] Propionibacterium acnes inhibition rate = (A - B) / A × 100%, where A is the average recovered colony of the control sample and B is the average recovered colony of the test sample.

[0087] 2. 5α-reductase inhibition rate, the test method is as follows: (1) Sample tube: Add 1 mL of the composition to be tested, 1 mL of enzyme solution (5α-reductase solution at 1.2 mmol / L), 1 mL of NADPH solution (reduced coenzyme II solution at 1.2 mmol / L), and 1 mL of testosterone solution (testosterone solution at 1.2 mmol / L) into a test tube, shake gently, pipette 220 μL into a 96-well microplate, make three parallels for each sample, place it in a microplate reader for detection, and measure the absorbance at 340 nm, which is the first measured value A. 1 After incubating the sample at 37 °C for 20 minutes, place it in a microplate reader for detection, and measure the absorbance at 340 nm, which is the second measured value A. 2 .

[0088] (2) Enzyme tube: Add 1 mL of PBS solution, 1 mL of enzyme solution (5α-reductase solution at 1.2 mmol / L), 1 mL of NADPH solution (reduced coenzyme II solution at 1.2 mmol / L), and 1 mL of testosterone solution (testosterone solution at 1.2 mmol / L) into a test tube, shake gently, pipette 200 μL into a 96-well microplate, make three parallels for each sample, place it in a microplate reader for detection, and measure the absorbance at 340 nm, which is the first measured value A. 3 After incubating the sample at 37 °C for 20 minutes, place it in a microplate reader for detection, and measure the absorbance at 340 nm, which is the second measured value A. 4 ; At the same time, perform a blank test, repeat the above experiment 3 times, and take the average.

[0089] 5α-reductase inhibition rate = 100% [1 - (A 1 - A 2 ) / (A 3 - A 4 ).

[0090] Table 1

[0091] It can be seen from Table 1 that the composition described in the present invention has excellent acne-removing effects.

[0092] By comparing Example 1 with Comparative Examples 1 - 8, it can be seen that the copper peptide, pepper fruit extract, and lime peel oil described in the present invention have a significant synergistic effect in acne removal. Lack of any one of them or replacement with other ingredients will lead to a significant decrease in the acne-removing effect.

[0093] By comparing Example 1 with Comparative Examples 9 - 10, it can be seen that the flower glue fruit extract prepared in the present invention has excellent acne-removing effects. The acne-removing effects of the flower glue fruit extracts obtained by different preparation methods are different, and using the flower glue fruit extract prepared in the present invention can more significantly improve the acne-removing effect.

[0094] Finally, it should be noted that the above embodiments are used to illustrate the technical solutions of the present invention rather than to limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A composition containing blue copper peptide, characterized in that, The invention comprises the following components in weight percentage: 0.05-0.5% p-hydroxyacetophenone, 0.05-0.5% thickener, 0.05-0.2% Centella asiatica extract, 0.1-0.5% tocopherol acetate, 0.1-0.5% dipotassium glycyrrhizinate, 0.2-0.6% lime peel oil, 0.2-1% blue copper peptide, 0.8-1.2% Zanthoxylum bungeanum fruit extract, 0.5-2% butylene glycol, 0.5-2% glycoside compound, 1-8% glyceryl polyether-26, 1-10% glycerol and the balance water.

2. The composition containing blue copper peptide according to claim 1, characterized in that The thickener comprises at least one of xanthan gum, carbomer, hydrolyzed sclerotium gum, taro gum, Chondrus crispus extract, and hydroxyethyl cellulose.

3. The composition containing blue copper peptide according to claim 1, characterized in that The glycoside compound includes at least one of glyceryl glucoside, coconut glucoside and decyl glucoside.

4. The composition containing blue copper peptide according to claim 1, characterized in that The preparation method of the Zanthoxylum bungeanum fruit extract comprises the following steps: (1) adding fish gum fruit powder, cellulase, and β-glucanase into water, performing a first enzymatic hydrolysis, and filtering to obtain a first enzymatic hydrolyzate; (2) adding ficin and alkaline protease to the first enzymatic hydrolysis solution, performing a second enzymatic hydrolysis, and obtaining a second enzymatic hydrolysis solution; (3) The second enzymatic hydrolyzate is first ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 300 Da, and then ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 3000 Da, and dried to obtain a Zanthoxylum bungeanum fruit extract.

5. The composition containing blue copper peptide according to claim 4, characterized in that The particle size of the flower gum fruit powder is 20-200 meshes.

6. The composition containing blue copper peptide according to claim 4, characterized in that The mass ratio of the fish gum fruit powder, cellulase, beta-glucanase and water is 1: (0.01-0.02): (0.002-0.006): (5-12).

7. The composition containing blue copper peptide according to claim 4, characterized in that The mass ratio of the ficin, alkaline protease and the first enzymatic hydrolysate is (0.005-0.012):(0.004-0.008):

1.

8. The composition containing blue copper peptide according to claim 4, characterized in that: The temperature of the first enzymolysis is 45-50° C., and the time of the first enzymolysis is 2-6 hours; the temperature of the second enzymolysis is 45-50° C., and the time of the second enzymolysis is 2-6 hours.

9. The method for preparing the composition containing blue copper peptide according to any one of claims 1 to 8, characterized in that: The following steps are involved: Add thickener, p-hydroxyacetophenone, butylene glycol, glycoside compounds, glyceryl polyether-26 and glycerin to water, heat to 65-80°C, stir evenly, cool to 40-45°C, add Centella asiatica extract, tocopherol acetate, dipotassium glycyrrhizate, lime peel oil, blue copper peptide and Zanthoxylum bungeanum fruit extract, stir evenly to obtain a composition containing blue copper peptide.

10. Use of the composition containing blue copper peptide according to any one of claims 1 to 8 in the preparation of a product with acne-removing effect.

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