Culture observation device for Chinese herbaceous peony disease resistance experiment

Abstract

The invention relates to the technical field of Chinese herbaceous peony disease resistance experiments, in particular to a culture and observation device for Chinese herbaceous peony disease resistance experiments, which comprises a casing, a culture cover, a ventilation sterilization mechanism, an illumination adjusting mechanism and an automatic taking mechanism, the top of the culture cover is provided with a flow port for air to pass through, and the bottom of the culture cover is provided with a through port for air to be discharged; the ventilation and sterilization mechanism is arranged at the top of the machine shell and comprises a first fixed disc, a rotating disc, a sealing cover and a first rotating driver; the first fixing disc is arranged on the top of the machine shell. According to the technical scheme, through the ventilation sterilization mechanism, the vertical laminar flow design that air enters from the top of the sealing cover and is exhausted from the bottom of the first fixing disc is adopted, mechanical damage to Chinese herbaceous peony caused by airflow disturbance can be reduced, air only enters through the ventilation opening formed in the top of the sealing cover, and cross contamination caused by disordered flowing is avoided.

Description

Technical Field

[0001] The present invention relates to the technical field of peony disease resistance experiments, and specifically relates to a cultivation and observation device for peony disease resistance experiments. Background Art

[0002] In the research field of peony disease resistance experiments, the cultivation and observation device is an indispensable key equipment. However, there are still some deficiencies in the design and function of the existing peony cultivation and observation devices, especially in terms of ventilation treatment and operation convenience. These problems have limited the experimental efficiency and data accuracy to a certain extent.

[0003] Specifically, traditional peony cultivation and observation devices often lack an efficient ventilation system. As a plant that is relatively sensitive to the growth environment, the performance of peony disease resistance is closely related to the ventilation conditions of the growth environment. Poor ventilation will not only affect the normal growth and development of peonies, but may also exacerbate the occurrence and spread of diseases. However, existing devices often ignore this point, resulting in the inability to provide suitable ventilation conditions for peonies during the experiment, thus affecting the accuracy of experimental results.

[0004] Chinese Patent Authorization Publication No. CN117016259B, a cultivation and observation device for vegetable disease resistance experiments, includes a cultivation and observation box, a vegetable cultivation tray, a root and stem observation mechanism, an auxiliary lifting mechanism, and a pathogen spraying mechanism. In this cultivation and observation device for vegetable disease resistance experiments of the present invention, when it is necessary to observe the situation on the back side of the root and stem of vegetables after inoculating pathogens, an image of the back side root and stem area of the vegetables is captured by a camera located between the support ring and the connection ring, and the captured image is displayed through a display. In addition, when the vegetables after inoculating pathogens are inclined, the distance between the three inclined reference plates on the upper surface of the connection ring and the vegetables can be observed. If there is a distance deviation between the distance between the vegetables and one of the inclined reference plates and the other two inclined reference plates, it indicates that the vegetables have an inclined problem, thus facilitating the experimenter to judge whether the vegetables are inclined after inoculating pathogens.

[0005] However, in actual use of the above structure, it relies on natural convection or simple mechanical fan air exchange, resulting in uncontrollable air flow direction, easy diffusion of pathogens with the air flow, and cross-contamination between samples. And there is a lack of filtration and sterilization modules during the air exchange process, so external microorganisms are easy to invade, and internal pathogens may contaminate the laboratory environment, thereby affecting the accuracy of the experiment. Summary of the Invention

[0006] In view of the above problems, a cultivation and observation device for peony disease resistance experiments is provided, which solves the problems of mechanical damage to peonies caused by air flow disturbance and cross-contamination caused by disordered air flow through a ventilation and sterilization mechanism.

[0007] To solve the problems of the existing technology, the present invention provides a cultivation and observation device for peony disease resistance experiments, including a machine shell and a cultivation cover. The cultivation and observation device further includes a ventilation and sterilization mechanism, a light adjustment mechanism, and an automatic picking and placing mechanism; a flow port for air passage is opened at the top of the cultivation cover, and a through port for air discharge is opened at the bottom of the cultivation cover; the ventilation and sterilization mechanism is arranged on the top of the machine shell, and the ventilation and sterilization mechanism includes a first fixed disk, a rotating disk, a sealing cover, and a first rotation driver; the first fixed disk is arranged on the top of the machine shell, and a plurality of first discharge ports for gas discharge are opened at the top of the first fixed disk, and the positions of the plurality of first discharge ports correspond to the through ports opened at the bottom of the cultivation cover; the rotating disk is rotatably arranged on the top of the machine shell and above the first fixed disk, and a plurality of second discharge ports corresponding to the positions of the discharge ports are opened at the top of the rotating disk, and a plurality of placement grooves for placing the cultivation cover are opened at the top of the rotating disk; the sealing cover is slidably arranged on the top of the rotating disk, and a plurality of ventilation ports corresponding to the positions of the second discharge ports are opened at the top of the sealing cover; the first rotation driver is arranged on the top of the machine shell and below the first fixed disk, and the output end of the first rotation driver is fixedly connected to the rotating disk; the light adjustment mechanism is arranged on the top of the machine shell and above the sealing cover, and the light adjustment mechanism is used for illuminating and heating the cultivation cover inside the sealing cover; the automatic picking and placing mechanism is arranged on the top of the machine shell and beside the light adjustment mechanism, and the automatic picking and placing mechanism is used for lifting the sealing cover and disengaging it from the outside of the rotating disk.

[0008] Preferably, the ventilation and sterilization mechanism further includes a filter box, a multi-stage filter plate, and an air inlet pipe; the filter box is arranged on the top of the sealing cover, and an opening is opened at the top of the filter box; the multi-stage filter plate is slidably arranged on the top of the filter box; the air inlet pipe is arranged outside the filter box and has a plurality of them, and the plurality of air inlet pipes are respectively connected to the ventilation ports opened at the top of the sealing cover.

[0009] Preferably, the ventilation and sterilization mechanism further includes a negative pressure generator, a sterilization box, and an ultraviolet lamp tube; a discharge chamber is opened at the top of the first fixed disk; the sterilization box is arranged inside the first fixed disk and communicated with the negative pressure generator, and a treatment chamber for gas recovery is opened inside the sterilization box; the negative pressure generator has a plurality of them and is respectively arranged inside the first fixed disk, and the plurality of negative pressure generators are respectively connected to the discharge ports opened at the top of the first fixed disk; the ultraviolet lamp tube has a plurality of them and is respectively arranged inside the sterilization box.

[0010] Preferably, the ventilation and sterilization mechanism further includes a drainage pipe and an exhaust fan; a pair of drainage pipes are respectively arranged outside the first fixed disk, and the drainage pipes are communicated with the sterilization box; a pair of exhaust fans are respectively arranged on the top of the machine shell and connected to the drainage pipes.

[0011] Preferably, the light adjusting mechanism includes a first linear driver, a lead screw, a sliding plate, a limiting block, and a displacement light block; the first linear driver is arranged on the top of the casing and beside the exhaust fan; the lead screw is rotatably arranged on the top of the casing and connected to the output end of the first linear driver; the sliding plate is slidably arranged on the top of the casing and threadedly connected to the lead screw; there are multiple limiting blocks which are respectively arranged on the top of the casing, and chutes are provided on the tops of the multiple limiting blocks; there are multiple displacement light blocks which are respectively slidably arranged on the tops of the limiting blocks.

[0012] Preferably, the light adjusting mechanism further includes light tubes and connecting rods; there are multiple light tubes which are respectively arranged on one side of the displacement light blocks, and the light directions of the multiple light tubes all face the sealing cover; the connecting rods are rotatably arranged on the tops of the displacement light blocks, one end of the connecting rod is rotatably connected to the displacement light block, and the other end of the connecting rod is rotatably connected to the sliding plate.

[0013] Preferably, the light adjusting mechanism further includes a limiting rod; there is a pair of limiting rods which are respectively arranged on the top of the casing, and there is a clearance fit between the sliding plate and the limiting rod.

[0014] Preferably, the automatic picking mechanism includes a second linear driver, a threaded rod, mounting blocks, and a displacement block; the second linear driver is arranged on the top of the casing and beside the rotating disk; the threaded rod is rotatably arranged on the top of the casing and connected to the output end of the second linear driver; there are a pair of mounting blocks which are respectively arranged on the top of the casing, and both of the pair of mounting blocks are beside the second linear driver; the displacement block is slidably arranged between the pair of mounting blocks and threadedly connected to the threaded rod.

[0015] Preferably, the automatic picking mechanism further includes telescopic cylinders and ejecting blocks; there are a pair of telescopic cylinders which are respectively arranged on the top of the displacement block; the ejecting block is arranged at the output end of the telescopic cylinder, and the ejecting block is T-shaped.

[0016] Preferably, the automatic picking mechanism further includes a connecting plate; the connecting plate is arranged outside the sealing cover, and the connecting plate is fixedly connected to the ejecting block.

[0017] The beneficial effects of the present invention compared with the prior art are as follows:

[0018] 1. By setting the ventilation and sterilization mechanism, the present invention adopts a vertical laminar flow design in which air enters from the top of the sealing cover and is exhausted through the bottom of the first fixed disk, which can reduce air flow disturbance and cause mechanical damage to peonies, and the air only enters through the ventilation openings provided at the top of the sealing cover, avoiding cross-contamination caused by disordered flow.

[0019] 2. By setting up a light intensity adjustment mechanism, during the seedling stage of peonies, low light intensity is required. At this time, multiple displacement light blocks are in a relatively distant state, and the light temperature of the light tubes is adjusted through a temperature controller to achieve low-light operation. When it comes to the adult stage of peonies, their growth characteristics are wide-spacing and high, and high light intensity is required. At this time, multiple displacement light blocks will approach relatively, and the light temperature of the light tubes is also adjusted through the temperature controller to achieve high-light operation. It realizes simulating natural light to adapt to different growth stages of peonies.

[0020] 3. By setting up an automatic picking and handling mechanism, the experimenter can quickly take out the culture cover. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 is the three-dimensional structure diagram from the first perspective of a culture observation device for peony disease resistance experiment of the present invention.

[0022] Figure 2 is the partial sectional three-dimensional structure diagram of a culture observation device for peony disease resistance experiment of the present invention.

[0023] Figure 3 is the top view structure diagram of a culture observation device for peony disease resistance experiment of the present invention.

[0024] Figure 4 is the three-dimensional structure diagram of the ventilation and sterilization mechanism of a culture observation device for peony disease resistance experiment of the present invention.

[0025] Figure 5 is the partial sectional three-dimensional structure diagram of the ventilation and sterilization mechanism of a culture observation device for peony disease resistance experiment of the present invention.

[0026] Figure 6 is Figure 5 the enlarged structure diagram at position A in

[0027] Figure 7 is Figure 5 the enlarged structure diagram at position B in

[0028] Figure 8 is the three-dimensional structure diagram of the sterilization box of a culture observation device for peony disease resistance experiment of the present invention.

[0029] Figure 9 is the three-dimensional structure diagram of the light intensity adjustment mechanism of a culture observation device for peony disease resistance experiment of the present invention.

[0030] Figure 10 is the three-dimensional structure diagram of the culture observation device for peony disease resistance experiment of the present invention when the displacement light blocks are in a relatively distant state.

[0031] Figure 11It is a three-dimensional structure diagram in the picking state of the automatic picking mechanism of a cultivation observation device for a peony disease resistance experiment of the present invention.

[0032] The reference numerals in the figure are: 1, housing; 2, cultivation cover; 21, through hole; 3, ventilation and sterilization mechanism; 31, first fixing plate; 311, first discharge port; 32, rotating disk; 322, second discharge port; 33, sealing cover; 331, ventilation opening; 34, first rotation driver; 35, filter box; 36, multi-stage filter plate; 37, intake pipe; 38, negative pressure generator; 39, sterilization box; 391, ultraviolet lamp tube; 392, drainage pipe; 393, exhaust fan; 4, light intensity adjustment mechanism; 41, first linear driver; 42, lead screw; 43, sliding plate; 44, limiting block; 45, displacement light block; 46, light tube; 47, connecting rod; 48, limiting rod; 5, automatic picking mechanism; 51, second linear driver; 52, threaded rod; 53, mounting block; 54, displacement block; 55, telescopic cylinder; 56, ejecting block; 57, connecting plate. Detailed implementation manners

[0033] In order to further understand the features, technical means, specific purposes and functions achieved by the present invention, the present invention will be described in further detail below with reference to the accompanying drawings and specific implementation manners.

[0034] See Figures 1 - 4As shown in the figure, a cultivation and observation device for peony disease resistance experiments includes a machine shell 1 and a cultivation cover 2. The cultivation and observation device further includes a ventilation and sterilization mechanism 3, a light adjustment mechanism 4, and an automatic picking and placing mechanism 5. A flow port for air passage is provided at the top of the cultivation cover 2, and a through port 21 for air discharge is provided at the bottom of the cultivation cover 2. The ventilation and sterilization mechanism 3 is arranged on the top of the machine shell 1. The ventilation and sterilization mechanism 3 includes a first fixed disk 31, a rotating disk 32, a sealing cover 33, and a first rotation driver 34. The first fixed disk 31 is arranged on the top of the machine shell 1, and a plurality of first discharge ports 311 for gas discharge are provided at the top of the first fixed disk 31. The positions of the plurality of first discharge ports 311 correspond to the positions of the through ports 21 provided at the bottom of the cultivation cover 2. The rotating disk 32 is rotatably arranged on the top of the machine shell 1 and is located above the first fixed disk 31. A plurality of second discharge ports 322 corresponding to the positions of the discharge ports are provided at the top of the rotating disk 32, and a plurality of placement grooves for placing the cultivation cover 2 are provided at the top of the rotating disk 32. The sealing cover 33 is slidably arranged on the top of the rotating disk 32, and a plurality of ventilation ports 331 corresponding to the positions of the second discharge ports 322 are provided at the top of the sealing cover 33. The first rotation driver 34 is arranged on the top of the machine shell 1 and is located below the first fixed disk 31, and the output end of the first rotation driver 34 is fixedly connected to the rotating disk 32. The light adjustment mechanism 4 is arranged on the top of the machine shell 1 and is located above the sealing cover 33. The light adjustment mechanism 4 is used for illuminating and heating the cultivation cover 2 inside the sealing cover 33. The automatic picking and placing mechanism 5 is arranged on the top of the machine shell 1 and is located beside the light adjustment mechanism 4, and the automatic picking and placing mechanism 5 is used for lifting the sealing cover 33 and disengaging it from the outside of the rotating disk 32.

[0035] The first fixed disk 31, the rotating disk 32, and the sealing cover 33 are all made of transparent glass material, which will not affect the observation effect of the peonies placed inside the cultivation cover 2. First, place the peonies to be cultivated inside the cultivation cover 2. Subsequently, use the automatic picking and placing mechanism 5 to lift the sealing cover 33 and keep a certain distance between the cultivation cover 2 and the rotating disk 32. Then, slide the peonies inside the cultivation cover 2 into the placement grooves provided on the top plate of the rotating disk 32. At this time, the installation of the cultivation cover 2 and the peonies is completed. The first fixed disk 31 is fixedly connected to the top of the machine shell 1, and under the drive of the first rotation driver 34, the sealing cover 33 will not rotate together with the rotating disk 32. In the initial state, the positions of the second discharge ports 322 provided at the top of the rotating disk 32 do not correspond to the positions of the first discharge ports 311 provided at the top of the first fixed disk 31. Instruction manual attachment Figure 5, which shows the states corresponding to the positions of the through holes, the second row of outlets 322, the second row of outlets 322, and the ventilation openings 331. When it is necessary to ventilate the culture hood 2 inside the seal hood 33, when it is necessary to ventilate the culture hood 2 inside the seal hood 33, first start the first rotary driver 34 and drive the rotary disk 32 to rotate. When the second row of outlets 322 at the top of the rotary disk 32 is completely aligned with the ventilation openings 331 at the top of the seal hood 33, the air circulation can be achieved. The vertical laminar flow design with air intake at the top of the seal hood 33 and exhaust through the bottom of the first fixed disk 31 is designed to reduce the mechanical damage to peonies caused by air flow disturbance. It can not only simulate the air flow dynamics in the natural environment but also ensure that the experimental isolation requirements are met. In addition, the air only enters through the ventilation openings 331 opened at the top of the seal hood 33, effectively avoiding the problem of cross-contamination caused by disordered flow.

[0036] See Figures 4 - 7 As shown, the ventilation and sterilization mechanism 3 further includes a filter box 35, a multi-stage filter plate 36, and an air inlet pipe 37; the filter box 35 is arranged at the top of the seal hood 33, and an opening is provided at the top of the filter box 35; the multi-stage filter plate 36 is slidably arranged at the top of the filter box 35; the air inlet pipe 37 is arranged outside the filter box 35 and there are multiple air inlet pipes 37, and the multiple air inlet pipes 37 are respectively connected to the ventilation openings 331 opened at the top of the seal hood 33.

[0037] When the positions of the second row of outlets opened at the top of the rotary disk 32 correspond to the ventilation openings 331 opened at the top of the seal hood 33, air can flow into the interior of the seal hood 33 through the multi-stage filter plate 36 and the air inlet pipe 37. The multi-stage filter plate 36 is preferably made of HEPA filter material, and the multi-stage filter plate 36 can renew the air and block the invasion of external pathogens or the spread of internal pathogens.

[0038] See Figures 5 - 7 As shown, the ventilation and sterilization mechanism 3 further includes a negative pressure generator 38, a sterilization box 39, and an ultraviolet lamp tube 391; a discharge chamber is provided at the top of the first fixed disk 31; the sterilization box 39 is arranged inside the first fixed disk 31 and is connected to the negative pressure generator 38, and a treatment chamber for gas recovery is provided inside the sterilization box 39; there are multiple negative pressure generators 38 and they are respectively arranged inside the first fixed disk 31, and the multiple negative pressure generators 38 are respectively connected to the discharge ports opened at the top of the first fixed disk 31; there are multiple ultraviolet lamp tubes 391 and they are respectively arranged inside the sterilization box 39.

[0039] When the position of the second discharge opening formed at the top of the rotating disk 32 corresponds to the position of the ventilation opening 331 formed at the top of the sealing cover 33, the negative pressure generator 38 located inside the first fixed disk 31 is activated. The air pressure difference drives external air to enter through the filter box 35 and the multi-stage filter plate 36, forming a directional air flow path from the outside to the inside of the sealing cover 33 and then discharging from the first discharge port 311, effectively avoiding cross-infection. When air intake is not required, only the negative pressure generator 38 needs to be turned off to stop the air intake. When exhaust is required, the rotating disk 32 rotates until the second discharge opening formed at the top of the rotating disk 32 does not coincide with the position of the ventilation opening 331 formed at the top of the sealing cover 33. Then, the negative pressure generator 38 continues to be activated and discharges the air inside the culture hood 2 into the inside of the sterilization box 39, and then the ultraviolet lamp tube 391 sterilizes the harmful components in the air. By adjusting the air intake volume of the negative pressure generator 38, the air intake can be indirectly affected. The negative pressure generator 38 is preferably a laboratory-grade model with precise pressure control, low noise, and corrosion resistance, such as the negative pressure generator 38 of the KNF model or the negative pressure generator 38 of the GAST model.

[0040] See Figure 7 and Figure 8 As shown, the ventilation and sterilization mechanism 3 further includes a drainage pipe 392 and an exhaust fan 393; there are a pair of drainage pipes 392, which are respectively arranged outside the first fixed disk 31, and the drainage pipe 392 is communicated with the sterilization box 39; there are a pair of exhaust fans 393, which are respectively arranged on the top of the housing 1 and are communicated with the drainage pipe 392.

[0041] After the harmful gas stays inside the sterilization box 39 for a period of time, the exhaust fan 393 is activated and discharges the sterilized air through the drainage pipe 392, realizing that the gas after ventilation can be sterilized and harmlessly discharged after ventilation.

[0042] See Figure 9 and Figure 10 As shown, the light intensity adjustment mechanism 4 includes a first linear actuator 41, a lead screw 42, a sliding plate 43, a limiting block 44, and a displacement light block 45; the first linear actuator 41 is arranged on the top of the housing 1 and beside the exhaust fan 393; the lead screw 42 is rotatably arranged on the top of the housing 1 and connected to the output end of the first linear actuator 41; the sliding plate 43 is slidably arranged on the top of the housing 1 and threadedly connected to the lead screw 42; there are multiple limiting blocks 44, which are respectively arranged on the top of the housing 1, and chutes are formed on the tops of the multiple limiting blocks 44; there are multiple displacement light blocks 45, which are respectively slidably arranged on the tops of the limiting blocks 44.

[0043] When it is necessary to irradiate the sealing cover 33 with light, first, the first linear driver 41 is activated to drive the lead screw 42 to rotate. When the lead screw 42 rotates, it can drive the sliding plate 43 to rise. When the sliding plate 43 descends, it can drive a plurality of displacement light blocks 45 to move relatively closer along the limiting block 44.

[0044] See Figure 9 and Figure 10 As shown in

[0045] and

[0046] See Figure 9 and Figure 10 The light adjusting mechanism 4 further includes a light tube 46 and a connecting rod 47. There are a plurality of light tubes 46, which are respectively arranged on one side of the displacement light block 45, and the light directions of the plurality of light tubes 46 all face the sealing cover 33. The connecting rod 47 is rotatably arranged on the top of the displacement light block 45. One end of the connecting rod 47 is rotatably connected to the displacement light block 45, and the other end of the connecting rod 47 is rotatably connected to the sliding plate 43.

[0047] A temperature controller and a temperature sensor are arranged on the top of the displacement light block 45. The temperature controller is used to adjust the illumination temperature of the light tube 46, and the temperature sensor is used to display and detect the illumination temperature of the light tube 46, and the temperature sensor is electrically connected to the temperature controller. When the sliding plate 43 rises, it can pull a plurality of rotatably connected displacement light blocks 45 and light tubes 46 to move towards the sealing cover 33, and then the light tubes 46 are activated to irradiate the culture cover 2 inside the sealing cover 33. In the seedling stage of peonies, low light intensity is required. At this time, the plurality of displacement light blocks 45 are in a relatively far - away state, and the illumination temperature of the light tubes 46 is adjusted through the temperature controller to achieve low - light operation. When it comes to the adult stage of peonies, its growth characteristics are wide - spaced, tall, and require high light intensity. At this time, the plurality of displacement light blocks 45 will move relatively closer, and the illumination temperature of the light tubes 46 is also adjusted through the temperature controller to achieve high - light operation. It realizes the simulation of natural light to adapt to different growth stages of peonies.

[0048] See Figure 11As shown, the automatic picking mechanism 5 includes a second linear driver 51, a threaded rod 52, a mounting block 53, and a displacement block 54. The second linear driver 51 is disposed on the top of the casing 1 and beside the rotating disk 32. The threaded rod 52 is rotatably disposed on the top of the casing 1 and connected to the output end of the second linear driver 51. There are a pair of mounting blocks 53 which are respectively disposed on the top of the casing 1, and both of the pair of mounting blocks 53 are beside the second linear driver 51. The displacement block 54 is slidably disposed between the pair of mounting blocks 53 and is in threaded connection with the threaded rod 52.

[0049] When picking the peonies inside the culture hood 2 is needed, first, the second linear driver 51 is activated to drive the rotation of the threaded rod 52. When the threaded rod 52 rotates, it can drive the displacement block 54 to move.

[0050] See Figure 11 As shown, the automatic picking mechanism 5 further includes a telescopic cylinder 55 and a jacking block 56. There are a pair of telescopic cylinders 55 which are respectively disposed on the top of the displacement block 54. The jacking block 56 is disposed at the output end of the telescopic cylinder 55, and the jacking block 56 is in a T shape.

[0051] When the displacement block 54 moves, it can drive the mounting block 53 and the telescopic cylinder 55 to move synchronously. At this time, the telescopic cylinder 55 jacks up the jacking block 56.

[0052] See Figure 11 As shown, the automatic picking mechanism 5 further includes a connecting plate 57. The connecting plate 57 is disposed outside the sealing cover 33, and the connecting plate 57 is fixedly connected to the jacking block 56.

[0053] When the jacking block 56 is jacked up, it can drive the connecting plate 57 to rise synchronously. When the connecting plate 57 rises, it can drive the sealing cover 33 to rise synchronously until the sealing cover 33 disengages from the outside of the rotating disk 32. Then, the displacement block 54 moves away from the rotating disk 32 along the mounting block 53. At this time, the experimenter can take out the culture hood 2.

[0054] Working principle: In the initial state, the second row of outlets 322 at the top of the rotating disk 32 does not correspond to the first row of outlets 311 at the top of the first fixed disk 31. When it is necessary to ventilate the culture hood 2 inside the sealing hood 33, the first rotation driver 34 is started to drive the rotation of the rotating disk 32. When the second row of outlets 322 at the top of the rotating disk 32 is completely aligned with the ventilation opening 331 at the top of the sealing hood 33, air can enter through the top of the sealing hood 33 and be discharged from the bottom of the first fixed disk 31, achieving a vertical laminar flow design, aiming to reduce the mechanical damage caused by air flow disturbance to peonies and at the same time meet the experimental isolation requirements. The air enters through the ventilation opening 331 at the top of the sealing hood 33, flows through the multi-stage filter plate 36 preferably made of HEPA filter material and the intake pipe 37 into the inside of the sealing hood 33, effectively avoiding cross-contamination caused by disordered flow. At this time, the negative pressure generator 38 inside the first fixed disk 31 is started, and the air pressure difference drives the external air to enter through the filter box 35 and the multi-stage filter plate 36, forming a directional air flow path, entering the sealing hood 33 from the outside and then being discharged from the first row of outlets 311, effectively avoiding cross-infection. When air intake is not required, the negative pressure generator 38 can be turned off to stop the air intake. When exhaust is required, the rotating disk 32 continues to rotate until the second row of outlets 322 does not coincide with the ventilation opening 331, and the negative pressure generator 38 continues to be started to discharge the air inside the culture hood 2 into the sterilization box 39, and the ultraviolet lamp tube 391 sterilizes the harmful components in the air. By adjusting the air intake volume of the negative pressure generator 38, the air intake volume can be indirectly controlled. The negative pressure generator 38 is preferably the laboratory-grade model of the KNF negative pressure generator 38 with precise pressure control, low noise, and corrosion resistance. After sterilization, the exhaust fan 393 is started and discharges the sterilized air through the drainage pipe 392 to achieve harmless emission. When it is necessary to illuminate the sealing hood 33, the first linear driver 41 is started to drive the rotation of the lead screw 42, and then drive the sliding plate 43 to rise or fall. The sliding plate 43 pulls a plurality of displacement lighting blocks 45 and lighting tubes 46 to move closer to or away from the sealing hood 33 through the connecting rod 47. A temperature controller and a temperature sensor are provided at the top of the displacement lighting block 45 for adjusting and displaying the lighting temperature of the lighting tube 46. At different growth stages of peonies, by adjusting the relative position and lighting temperature of the displacement lighting block 45, the simulation of natural light is realized to meet the growth requirements of peonies. When the sliding plate 43 moves, it moves stably along the limiting rod 48 to improve the moving stability. When it is necessary to take the peonies inside the culture hood 2, the second linear driver 51 is started to drive the rotation of the threaded rod 52, and then drive the displacement block 54 to move. The displacement block 54 drives the mounting block 53 and the telescopic cylinder 55 to move synchronously, and the telescopic cylinder 55 jacks up the ejector block 56, and then drives the connecting plate 57 and the sealing hood 33 to rise synchronously. Until the sealing hood 33 is separated from the outside of the rotating disk 32, the displacement block 54 moves away from the rotating disk 32 along the mounting block 53. At this time, the experimenter can take out the culture hood 2.

[0055] The above embodiments merely represent one or several implementation manners of the present invention. The description thereof is relatively specific and detailed, but it should not be construed as a limitation on the scope of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, several modifications and improvements can still be made, and these all fall within the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the appended claims.

Claims

1. A culture observation device for peony disease resistance experiment, comprising a housing (1) and a culture cover (2), characterized in that: The culture observation device also includes a ventilation and sterilization mechanism (3), a light adjustment mechanism (4) and an automatic taking mechanism (5); The top of the culture cover (2) is provided with a flow port for air to pass through, and the bottom of the culture cover (2) is provided with a through hole (21) for air to be discharged; The ventilation and sterilization mechanism (3) is arranged on the top of the housing (1), and comprises a first fixed disk (31), a rotating disk (32), a sealing cover (33) and a first rotating driver (34); The first fixed plate (31) is arranged on the top of the housing (1), and a plurality of first discharge ports (311) for discharging gas are provided on the top of the first fixed plate (31), and the plurality of first discharge ports (311) correspond to the positions of the through ports (21) provided on the bottom of the culture cover (2); The rotating disk (32) is rotatably arranged on the top of the housing (1) and is located above the first fixed disk (31), and a plurality of second discharge ports (322) corresponding to the positions of the discharge ports are provided on the top of the rotating disk (32), and a plurality of placement grooves for placing the culture cover (2) are provided on the top of the rotating disk (32); The sealing cover (33) is slidably disposed on the top of the rotating disk (32), and a plurality of ventilation holes (331) corresponding to the positions of the second discharge ports (322) are opened on the top of the sealing cover (33); The first rotary driver (34) is arranged on the top of the housing (1) and is located below the first fixed disk (31), and the output end of the first rotary driver (34) is fixedly connected to the rotary disk (32); The light adjustment mechanism (4) is arranged on the top of the housing (1) and is located above the sealing cover (33), and the light adjustment mechanism (4) is used to perform light heating on the culture cover (2) inside the sealing cover (33); The automatic taking mechanism (5) is arranged on the top of the housing (1) and is located beside the light adjustment mechanism (4), and the automatic taking mechanism (5) is used to lift the sealing cover (33) and separate it from the outside of the rotating disk (32).

2. The cultivation and observation device for peony disease resistance experiment according to claim 1, characterized in that: The ventilation and sterilization mechanism (3) further comprises a filter box (35), a multi-stage filter plate (36) and an air intake pipe (37); the filter box (35) is arranged on the top of the sealing cover (33), and an opening is provided on the top of the filter box (35); the multi-stage filter plate (36) is slidably arranged on the top of the filter box (35); the air intake pipe (37) is arranged outside the filter box (35) and has a plurality of them, and the plurality of air intake pipes (37) are respectively connected to the ventilation openings (331) provided on the top of the sealing cover (33).

3. The cultivation and observation device for peony disease resistance experiment according to claim 1, characterized in that: The ventilation and sterilization mechanism (3) also includes a negative pressure generator (38), a sterilization box (39) and an ultraviolet lamp (391); a discharge chamber is provided at the top of the first fixed disk (31); the sterilization box (39) is arranged inside the first fixed disk (31) and is connected to the negative pressure generator (38); a processing chamber for gas recovery is provided inside the sterilization box (39); there are multiple negative pressure generators (38) which are respectively arranged inside the first fixed disk (31), and the multiple negative pressure generators (38) are respectively connected to the discharge ports provided at the top of the first fixed disk (31); there are multiple ultraviolet lamps (391) which are respectively arranged inside the sterilization box (39).

4. The cultivation and observation device for peony disease resistance experiment according to claim 1, characterized in that: The ventilation and sterilization mechanism (3) further comprises a drainage pipe (392) and an exhaust fan (393); the drainage pipe (392) comprises a pair and is respectively arranged outside the first fixed plate (31), and the drainage pipe (392) is connected to the sterilization box (39); the exhaust fan (393) comprises a pair and is respectively arranged at the top of the casing (1) and is connected to the drainage pipe (392).

5. The cultivation and observation device for peony disease resistance experiment according to claim 1, characterized in that: The light adjustment mechanism (4) comprises a first linear drive (41), a screw rod (42), a sliding plate (43), a limiting block (44) and a displacement light block (45); the first linear drive (41) is arranged at the top of the housing (1) and is located beside the exhaust fan (393); the screw rod (42) is rotatably arranged at the top of the housing (1) and is connected to the output end of the first linear drive (41); the sliding plate (43) is slidably arranged at the top of the housing (1) and is threadedly connected to the screw rod (42); there are a plurality of limiting blocks (44) which are respectively arranged at the top of the housing (1), and a slide groove is provided on the top of each of the plurality of limiting blocks (44); there are a plurality of displacement light blocks (45) which are respectively slidably arranged on the top of the limiting blocks (44).

6. The cultivation and observation device for peony disease resistance experiment according to claim 5, characterized in that: The illumination adjustment mechanism (4) further comprises an illumination lamp tube (46) and a connecting rod (47); the illumination lamp tubes (46) are provided in plurality and are respectively arranged on one side of the displacement illumination block (45), and the illumination directions of the plurality of illumination lamp tubes (46) are all oriented toward the sealing cover (33); the connecting rod (47) is rotatably arranged on the top of the displacement illumination block (45), one end of the connecting rod (47) is rotatably connected to the displacement illumination block (45), and the other end of the connecting rod (47) is rotatably connected to the sliding plate (43).

7. The cultivation and observation device for peony disease resistance experiment according to claim 5, characterized in that: The light adjustment mechanism (4) further comprises a limiting rod (48); the limiting rod (48) comprises a pair and is respectively arranged on the top of the housing (1), and a clearance fit is formed between the sliding plate (43) and the limiting rod (48).

8. The cultivation and observation device for peony disease resistance experiment according to claim 1, characterized in that: The automatic taking mechanism (5) comprises a second linear drive (51), a threaded rod (52), a mounting block (53) and a displacement block (54); the second linear drive (51) is arranged at the top of the housing (1) and is located beside the rotating disk (32); the threaded rod (52) is rotatably arranged at the top of the housing (1) and is connected to the output end of the second linear drive (51); the mounting block (53) has a pair and is respectively arranged at the top of the housing (1), and the pair of mounting blocks (53) are both located beside the second linear drive (51); the displacement block (54) is slidably arranged between the pair of mounting blocks (53) and is threadedly connected to the threaded rod (52).

9. The cultivation and observation device for peony disease resistance experiment according to claim 8, characterized in that: The automatic taking mechanism (5) further comprises a telescopic cylinder (55) and an ejection block (56); the telescopic cylinder (55) has a pair of and is respectively arranged on the top of the displacement block (54); the ejection block (56) is arranged at the output end of the telescopic cylinder (55), and the ejection block (56) is T-shaped.

10. The cultivation and observation device for peony disease resistance experiment according to claim 8, characterized in that: The automatic taking mechanism (5) further comprises a connecting plate (57); the connecting plate (57) is arranged outside the sealing cover (33), and the connecting plate (57) is fixedly connected to the ejection block (56).

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