A method for enhancing the body color of east starfish

Abstract

The application discloses a culture method for enhancing the body color of Eaststar Prawn and relates to the technical field of aquaculture. The method comprises the following steps: (1) light induction: white-red-blue-yellow light sources are alternately used for irradiation, (2) color enhancer feeding: the color enhancer is fed simultaneously with the basic feed, and the components of the color enhancer include Camellia nitidissima polysaccharide, Sargassum fusiforme polysaccharide, fucoxanthin, scallop peptide, silymarin, guanidino acetic acid and sodium butyrate, and (3) water flow stimulation. Through the regulation of physiological metabolism, nutrient absorption and environmental adaptability, the method jointly acts on the body color performance of Eaststar Prawn, is favorable for enhancing the body color of Eaststar Prawn and improving the economic value of Eaststar Prawn.

Description

Technical Field

[0001] This invention relates to the field of aquaculture technology, and in particular to a method for enhancing the color of the Chinese starfish. Background Technology

[0002] The Eastern Star Grouper, scientifically known as *Scoidea leopardina*, belongs to the order Perciformes, suborder Perciformes, family Serranidae, subfamily Scoideinae, and genus *Scoidea*. It is a warm-water, reef-dwelling carnivorous fish. Its colors include blue, red, brown, and yellow. It has a more slender body than typical grouper, with a small head. The body and head range from olive to reddish-brown, covered with tiny blue spots. The pectoral fins are red, and the caudal fin has a faint dark band along its posterior edge. Its vibrant colors, with black pupils in its blue eyes and fine spots resembling stars, give it the common name "Star Grouper." The Eastern Star Grouper has white, tender, and nutritious flesh, making it a highly prized and premium edible fish, favored by many consumers. However, due to the prevalence of high-density aquaculture, coupled with deteriorating aquaculture environments and genetic degradation, the color of artificially farmed Eastern Star Grouper has severely deteriorated, becoming paler and significantly impacting its quality and market price. Therefore, there is an urgent need for a farming method that can significantly enhance the color of the Eastern Star Grouper. Summary of the Invention

[0003] In view of the above-mentioned deficiencies in the prior art, the present invention proposes a breeding method to enhance the body color of the Oriental Star Spot, so as to solve the problems mentioned in the background art.

[0004] To achieve the above objectives, the present invention provides the following technical solution:

[0005] A breeding method for enhancing the coloration of the Oriental Star Spot includes the following steps:

[0006] (1) Illumination induction: Alternating white-red-blue-yellow light sources were used for illumination;

[0007] (2) Color enhancer feeding: Feed simultaneously with the basic feed. The components of the color enhancer include Camellia chrysantha polysaccharide, Sargassum fusiforme polysaccharide, fucoxanthin, scallop peptide, silymarin, guanidinoacetic acid and sodium butyrate. When the pH of the aquaculture water is 7.2-7.7, the amount of color enhancer fed is 0.8-1.5% of the fish body weight; when the pH of the aquaculture water is 7.8-8.2, the amount of color enhancer fed is 0.15-0.35% of the fish body weight; when the pH of the aquaculture water is 8.3-8.6, the amount of color enhancer fed is 0.50-1.0% of the fish body weight.

[0008] (3) Water flow stimulation: The water body is kept at a flow rate of 0.10-0.15 m / s for 2-4 hours, at a flow rate of 0.20-0.30 m / s for 1-3 hours, at a flow rate of 0.35-0.40 m / s for 0.5-1.5 hours, and the flow is stopped at other times.

[0009] Preferably, the illumination time of the white light source in step (1) is the same as the total illumination time of the red-blue-yellow light source.

[0010] Preferably, in step (1), the illumination time of white light source is 06:00 to 18:00, the illumination time of red light source is 18:00 to 02:00, the illumination time of blue light source is 02:00 to 04:00, and the illumination time of yellow light source is 04:00 to 06:00.

[0011] Preferably, in step (1), the irradiation intensity of the white light source is 100-150 lux, the irradiation intensity of the red light source is 500-1000 lux, the irradiation intensity of the blue light source is 300-500 lux, and the irradiation intensity of the yellow light source is 200-300 lux.

[0012] Preferably, the wavelength range of the white light source is 500-600nm, the wavelength range of the red light source is 610-650nm, the wavelength range of the blue light source is 450-480nm, and the wavelength range of the yellow light source is 580-590nm.

[0013] Preferably, the components of the basic feed in step (2) include, by weight, 30-40 parts white fish meal, 20-35 parts earthworm meal, 10-15 parts corn gluten meal, 7-12 parts soybean meal, 5-10 parts soybean oil, 2-7 parts choline chloride and 1-6 parts vitamin C.

[0014] Preferably, the amount of basic feed in step (2) is 3 to 5% of the fish's body weight.

[0015] Preferably, in step (2), the colorant is obtained by mixing and sieving the components by weight, extruding, puffing, granulating and drying. The components include 20-30 parts of Camellia chrysantha polysaccharide, 20-25 parts of Sargassum fusiforme polysaccharide, 10-18 parts of fucoxanthin, 8-12 parts of scallop peptide, 4-10 parts of silymarin, 1-3 parts of guanidinoacetic acid and 1-3 parts of sodium butyrate.

[0016] Preferably, the color enhancer in step (2) comprises, by weight, 25 parts of Camellia chrysantha polysaccharide, 22 parts of Sargassum fusiforme polysaccharide, 15 parts of fucoxanthin, 10 parts of scallop peptide, 6 parts of silymarin, 2 parts of guanidinoacetic acid and 2 parts of sodium butyrate.

[0017] Preferably, in step (2), when the pH of the aquaculture water is 7.2 to 7.7, the amount of color enhancer fed is 1.2% of the fish's body weight; when the pH of the aquaculture water is 7.8 to 8.2, the amount of color enhancer fed is 0.30% of the fish's body weight; and when the pH of the aquaculture water is 8.3 to 8.6, the amount of color enhancer fed is 0.80% of the fish's body weight.

[0018] Preferably, in step (3), the water body is kept at a flow rate of 0.12 m / s for 3 hours, at a flow rate of 0.25 m / s for 2 hours, and at a flow rate of 0.38 m / s for 1 hour each day, and the water flow is stopped for the rest of the time. The flow rate of the water is controlled by a 500W variable frequency water pump installed in the pool.

[0019] Compared with the prior art, the beneficial effects of the present invention are:

[0020] (1) Appropriate alternating light can promote the activity of pigment cells in the grouper, especially the synthesis and deposition of carotenoids. By regulating the photoperiod and light intensity, the light changes in the habitat can be simulated, which can reduce the stress response of the fish and thus maintain the stability and brightness of the body color.

[0021] (2) Adding color enhancers containing carotenoids (such as fucoxanthin) to the feed can directly promote the accumulation of pigments on the fish's skin. At the same time, by linking the amount of color enhancer to the pH of the aquaculture water, and by using specific aquaculture feeding techniques and strategies, the digestibility and utilization rate of protein in the basic feed can be improved, the nutrient absorption efficiency can be enhanced, and the pigment metabolism can be accelerated or the synthesis and color development of pigments can be indirectly supported.

[0022] (3) Moderate stimulation from circulating water can enhance the activity level of the grouper, promoting blood circulation and metabolism. This process helps to distribute pigments evenly and reduces the dullness of body color caused by environmental stress, giving the fish a natural and bright color. Detailed Implementation

[0023] The technical solution of the present invention will be further described in detail below with reference to specific embodiments.

[0024] Example 1

[0025] A breeding method for enhancing the coloration of the Oriental Star Spot includes the following steps:

[0026] (1) Construction of breeding ponds: Indoor breeding ponds are constructed, and the breeding ponds are disinfected with a mixture of bleaching powder and chlorine dioxide before stocking the fish fry.

[0027] (2) Stocking fry: The water temperature in the rearing pond should be 22-29℃, the salinity 25-32‰, and the dissolved oxygen above 5mg / L. Select healthy, disease-free, and injury-free grouper fry of 2.5-3cm and stock them in the rearing pond at a rate of 100-200 fry / m². 3 Before releasing the grouper fry, soak them in salt water for 10 minutes to disinfect them.

[0028] (3) Light induction: After the fish fry are released, they are irradiated with alternating white-red-blue-yellow light sources. The irradiation time of white light source is the same as the total irradiation time of red-blue-yellow light source. The irradiation time of white light source is 06:00-18:00 with an irradiation intensity of 100 lux. The irradiation time of red light source is 18:00-02:00 with an irradiation intensity of 500 lux. The irradiation time of blue light source is 02:00-04:00 with an irradiation intensity of 300 lux. The irradiation time of yellow light source is 04:00-06:00 with an irradiation intensity of 200 lux.

[0029] (4) Color enhancer administration: Administer simultaneously with the basic feed, three times daily at 09:00, 13:00, and 18:00. The color enhancer components, by weight, include 20 parts of Camellia chrysantha polysaccharide, 20 parts of Sargassum fusiforme polysaccharide, 10 parts of fucoxanthin, 8 parts of scallop peptide, 4 parts of silymarin, 1 part of guanidinoacetic acid, and 1 part of sodium butyrate. When the pH of the aquaculture water is 7.2–7.7, the color enhancer dosage is 0.8% of the fish's body weight. When the pH of the aquaculture water is 7.8–8.2, the amount of color enhancer fed is 0.15% of the fish's body weight; when the pH of the aquaculture water is 8.3–8.6, the amount of color enhancer fed is 0.50% of the fish's body weight; the amount of the basic feed fed is 3% of the fish's body weight, and the components of the basic feed, by weight, include 30 parts white fish meal, 20 parts earthworm meal, 10 parts corn gluten meal, 7 parts soybean meal, 5 parts soybean oil, 2 parts choline chloride and 1 part vitamin C;

[0030] (5) Water flow stimulation: The water body is kept at a flow rate of 0.10 m / s for 2 hours, 0.20 m / s for 1 hour, and 0.35 m / s for 0.5 hours every day, and the water flow is stopped at other times.

[0031] Example 2

[0032] A breeding method for enhancing the coloration of the Oriental Star Spot includes the following steps:

[0033] (1) Construction of breeding ponds: Indoor breeding ponds are constructed, and the breeding ponds are disinfected with a mixture of bleaching powder and chlorine dioxide before stocking the fish fry.

[0034] (2) Stocking fry: The water temperature in the rearing pond should be 22-29℃, the salinity 25-32‰, and the dissolved oxygen above 5mg / L. Select healthy, disease-free, and injury-free grouper fry of 2.5-3cm and stock them in the rearing pond at a rate of 100-200 fry / m². 3 Before releasing the grouper fry, soak them in salt water for 10 minutes to disinfect them.

[0035] (3) Light induction: After the fish fry are released, they are irradiated with alternating white-red-blue-yellow light sources. The irradiation time of white light source is the same as the total irradiation time of red-blue-yellow light source. The irradiation time of white light source is 06:00-18:00 with an irradiation intensity of 150 lux. The irradiation time of red light source is 18:00-02:00 with an irradiation intensity of 1000 lux. The irradiation time of blue light source is 02:00-04:00 with an irradiation intensity of 500 lux. The irradiation time of yellow light source is 04:00-06:00 with an irradiation intensity of 300 lux.

[0036] (4) Color enhancer feeding: Feed simultaneously with the basic feed, 3 times a day, at 09:00, 13:00 and 18:00. The components of the color enhancer, by weight, include 30 parts of Camellia chrysantha polysaccharide, 25 parts of Sargassum fusiforme polysaccharide, 18 parts of fucoxanthin, 12 parts of scallop peptide, 10 parts of silymarin, 3 parts of guanidinoacetic acid and 3 parts of sodium butyrate. When the pH of the aquaculture water is 7.2 to 7.7, the amount of color enhancer fed is 1.5% of the fish's body weight. When the pH of the aquaculture water is 7.8–8.2, the dosage of the color enhancer is 0.35% of the fish's body weight; when the pH of the aquaculture water is 8.3–8.6, the dosage of the color enhancer is 1.0% of the fish's body weight; the dosage of the basic feed is 5% of the fish's body weight, and the components of the basic feed, by weight, include 40 parts white fish meal, 35 parts earthworm meal, 15 parts corn gluten meal, 12 parts soybean meal, 10 parts soybean oil, 7 parts choline chloride, and 6 parts vitamin C;

[0037] (5) Water flow stimulation: The water body is kept at a flow rate of 0.15 m / s for 4 hours, 0.30 m / s for 3 hours, and 0.40 m / s for 1.5 hours every day, and the water flow is stopped at other times.

[0038] Example 3

[0039] A breeding method for enhancing the coloration of the Oriental Star Spot includes the following steps:

[0040] (1) Construction of breeding ponds: Indoor breeding ponds are constructed, and the breeding ponds are disinfected with a mixture of bleaching powder and chlorine dioxide before stocking the fish fry.

[0041] (2) Stocking fry: The water temperature in the rearing pond should be 22-29℃, the salinity 25-32‰, and the dissolved oxygen above 5mg / L. Select healthy, disease-free, and injury-free grouper fry of 2.5-3cm and stock them in the rearing pond at a rate of 100-200 fry / m². 3 Before releasing the grouper fry, soak them in salt water for 10 minutes to disinfect them.

[0042] (3) Light induction: After the fish fry are released, they are irradiated with alternating white-red-blue-yellow light sources. The irradiation time of white light source is the same as the total irradiation time of red-blue-yellow light source. The irradiation time of white light source is 06:00-18:00 with an irradiation intensity of 120 lux. The irradiation time of red light source is 18:00-02:00 with an irradiation intensity of 600 lux. The irradiation time of blue light source is 02:00-04:00 with an irradiation intensity of 400 lux. The irradiation time of yellow light source is 04:00-06:00 with an irradiation intensity of 250 lux.

[0043] (4) Color enhancer administration: Administer simultaneously with the basic feed, three times daily at 09:00, 13:00, and 18:00. The color enhancer components, by weight, include 25 parts of Camellia chrysantha polysaccharide, 22 parts of Sargassum fusiforme polysaccharide, 15 parts of fucoxanthin, 10 parts of scallop peptide, 6 parts of silymarin, 2 parts of guanidinoacetic acid, and 2 parts of sodium butyrate. When the pH of the aquaculture water is 7.2–7.7, the color enhancer dosage is 1.2% of the fish's body weight. When the pH of the aquaculture water is 7.8–8.2, the amount of color enhancer fed is 0.30% of the fish's body weight; when the pH of the aquaculture water is 8.3–8.6, the amount of color enhancer fed is 0.80% of the fish's body weight; the amount of basic feed fed is 4% of the fish's body weight, and the components of the basic feed, by weight, include 35 parts white fish meal, 30 parts earthworm meal, 12 parts corn gluten meal, 10 parts soybean meal, 8 parts soybean oil, 5 parts choline chloride and 4 parts vitamin C;

[0044] (5) Water flow stimulation: The water body is kept at a flow rate of 0.12 m / s for 3 hours, 0.25 m / s for 2 hours, and 0.38 m / s for 1 hour every day, and the water flow is stopped at other times.

[0045] Comparative Example 1

[0046] The difference between this comparative example and Example 3 is that there is no light source for red-blue-yellow induction, while the other components and steps remain the same.

[0047] A breeding method for enhancing the coloration of the Oriental Star Spot includes the following steps:

[0048] (1) Construction of breeding ponds: Indoor breeding ponds are constructed, and the breeding ponds are disinfected with a mixture of bleaching powder and chlorine dioxide before stocking the fish fry.

[0049] (2) Stocking fry: The water temperature in the rearing pond should be 22-29℃, the salinity 25-32‰, and the dissolved oxygen above 5mg / L. Select healthy, disease-free, and injury-free grouper fry of 2.5-3cm and stock them in the rearing pond at a rate of 100-200 fry / m². 3 Before releasing the grouper fry, soak them in salt water for 10 minutes to disinfect them.

[0050] (3) Light induction: After the fish fry are released, they are exposed to white light all day with an intensity of 120 lux.

[0051] (4) Color enhancer administration: Administer simultaneously with the basic feed, three times daily at 09:00, 13:00, and 18:00. The color enhancer components, by weight, include 25 parts of Camellia chrysantha polysaccharide, 22 parts of Sargassum fusiforme polysaccharide, 15 parts of fucoxanthin, 10 parts of scallop peptide, 6 parts of silymarin, 2 parts of guanidinoacetic acid, and 2 parts of sodium butyrate. When the pH of the aquaculture water is 7.2–7.7, the color enhancer dosage is 1.2% of the fish's body weight. When the pH of the aquaculture water is 7.8–8.2, the amount of color enhancer fed is 0.30% of the fish's body weight; when the pH of the aquaculture water is 8.3–8.6, the amount of color enhancer fed is 0.80% of the fish's body weight; the amount of basic feed fed is 4% of the fish's body weight, and the components of the basic feed, by weight, include 35 parts white fish meal, 30 parts earthworm meal, 12 parts corn gluten meal, 10 parts soybean meal, 8 parts soybean oil, 5 parts choline chloride and 4 parts vitamin C;

[0052] (5) Water flow stimulation: The water body is kept at a flow rate of 0.12 m / s for 3 hours, 0.25 m / s for 2 hours, and 0.38 m / s for 1 hour every day, and the water flow is stopped at other times.

[0053] Comparative Example 2

[0054] The difference between this comparative example and Example 3 is that there is no light source for red induction, while the other components and steps remain unchanged.

[0055] A breeding method for enhancing the coloration of the Oriental Star Spot includes the following steps:

[0056] (1) Construction of breeding ponds: Indoor breeding ponds are constructed, and the breeding ponds are disinfected with a mixture of bleaching powder and chlorine dioxide before stocking the fish fry.

[0057] (2) Stocking fry: The water temperature in the rearing pond should be 22-29℃, the salinity 25-32‰, and the dissolved oxygen above 5mg / L. Select healthy, disease-free, and injury-free grouper fry of 2.5-3cm and stock them in the rearing pond at a rate of 100-200 fry / m². 3 Before releasing the grouper fry, soak them in salt water for 10 minutes to disinfect them.

[0058] (3) Light induction: After the fish fry are released, they are irradiated with alternating white, blue and yellow light sources. The irradiation time of white light source is the same as the total irradiation time of blue and yellow light source. The irradiation time of white light source is 06:00 to 18:00, and the irradiation intensity is 120 lux. The irradiation time of blue light source is 18:00 to 24:00, and the irradiation intensity is 400 lux. The irradiation time of yellow light source is 24:00 to 06:00, and the irradiation intensity is 250 lux.

[0059] (4) Color enhancer administration: Administer simultaneously with the basic feed, three times daily at 09:00, 13:00, and 18:00. The color enhancer components, by weight, include 25 parts of Camellia chrysantha polysaccharide, 22 parts of Sargassum fusiforme polysaccharide, 15 parts of fucoxanthin, 10 parts of scallop peptide, 6 parts of silymarin, 2 parts of guanidinoacetic acid, and 2 parts of sodium butyrate. When the pH of the aquaculture water is 7.2–7.7, the color enhancer dosage is 1.2% of the fish's body weight. When the pH of the aquaculture water is 7.8–8.2, the amount of color enhancer fed is 0.30% of the fish's body weight; when the pH of the aquaculture water is 8.3–8.6, the amount of color enhancer fed is 0.80% of the fish's body weight; the amount of basic feed fed is 4% of the fish's body weight, and the components of the basic feed, by weight, include 35 parts white fish meal, 30 parts earthworm meal, 12 parts corn gluten meal, 10 parts soybean meal, 8 parts soybean oil, 5 parts choline chloride and 4 parts vitamin C;

[0060] (5) Water flow stimulation: The water body is kept at a flow rate of 0.12 m / s for 3 hours, 0.25 m / s for 2 hours, and 0.38 m / s for 1 hour every day, and the water flow is stopped at other times.

[0061] Comparative Example 3

[0062] The difference between this comparative example and Example 3 is that there is no light source for blue induction, while the other components and steps remain unchanged.

[0063] A breeding method for enhancing the coloration of the Oriental Star Spot includes the following steps:

[0064] (1) Construction of breeding ponds: Indoor breeding ponds are constructed, and the breeding ponds are disinfected with a mixture of bleaching powder and chlorine dioxide before stocking the fish fry.

[0065] (2) Stocking fry: The water temperature in the rearing pond should be 22-29℃, the salinity 25-32‰, and the dissolved oxygen above 5mg / L. Select healthy, disease-free, and injury-free grouper fry of 2.5-3cm and stock them in the rearing pond at a rate of 100-200 fry / m². 3 Before releasing the grouper fry, soak them in salt water for 10 minutes to disinfect them.

[0066] (3) Light induction: After the fish fry are released, they are irradiated with alternating white-red-yellow light sources. The irradiation time of white light source is the same as the total irradiation time of red-yellow light source. The irradiation time of white light source is 06:00-18:00 with an irradiation intensity of 120 lux. The irradiation time of red light source is 18:00-02:00 with an irradiation intensity of 600 lux. The irradiation time of yellow light source is 02:00-06:00 with an irradiation intensity of 250 lux.

[0067] (4) Color enhancer administration: Administer simultaneously with the basic feed, three times daily at 09:00, 13:00, and 18:00. The color enhancer components, by weight, include 25 parts of Camellia chrysantha polysaccharide, 22 parts of Sargassum fusiforme polysaccharide, 15 parts of fucoxanthin, 10 parts of scallop peptide, 6 parts of silymarin, 2 parts of guanidinoacetic acid, and 2 parts of sodium butyrate. When the pH of the aquaculture water is 7.2–7.7, the color enhancer dosage is 1.2% of the fish's body weight. When the pH of the aquaculture water is 7.8–8.2, the amount of color enhancer fed is 0.30% of the fish's body weight; when the pH of the aquaculture water is 8.3–8.6, the amount of color enhancer fed is 0.80% of the fish's body weight; the amount of basic feed fed is 4% of the fish's body weight, and the components of the basic feed, by weight, include 35 parts white fish meal, 30 parts earthworm meal, 12 parts corn gluten meal, 10 parts soybean meal, 8 parts soybean oil, 5 parts choline chloride and 4 parts vitamin C;

[0068] (5) Water flow stimulation: The water body is kept at a flow rate of 0.12 m / s for 3 hours, 0.25 m / s for 2 hours, and 0.38 m / s for 1 hour every day, and the water flow is stopped at other times.

[0069] Comparative Example 4

[0070] The difference between this comparative example and Example 3 is that there is no light source to induce yellow color, while the other components and steps remain unchanged.

[0071] A breeding method for enhancing the coloration of the Oriental Star Spot includes the following steps:

[0072] (1) Construction of breeding ponds: Indoor breeding ponds are constructed, and the breeding ponds are disinfected with a mixture of bleaching powder and chlorine dioxide before stocking the fish fry.

[0073] (2) Stocking fry: The water temperature in the rearing pond should be 22-29℃, the salinity 25-32‰, and the dissolved oxygen above 5mg / L. Select healthy, disease-free, and injury-free grouper fry of 2.5-3cm and stock them in the rearing pond at a rate of 100-200 fry / m². 3 Before releasing the grouper fry, soak them in salt water for 10 minutes to disinfect them.

[0074] (3) Light induction: After the fish fry are released, they are irradiated with alternating white-red-blue light sources. The irradiation time of white light source is the same as the total irradiation time of red-blue-yellow light source. The irradiation time of white light source is 06:00-18:00, and the irradiation intensity is 120 lux. The irradiation time of red light source is 18:00-02:00, and the irradiation intensity is 600 lux. The irradiation time of blue light source is 02:00-06:00, and the irradiation intensity is 400 lux.

[0075] (4) Color enhancer administration: Administer simultaneously with the basic feed, three times daily at 09:00, 13:00, and 18:00. The color enhancer components, by weight, include 25 parts of Camellia chrysantha polysaccharide, 22 parts of Sargassum fusiforme polysaccharide, 15 parts of fucoxanthin, 10 parts of scallop peptide, 6 parts of silymarin, 2 parts of guanidinoacetic acid, and 2 parts of sodium butyrate. When the pH of the aquaculture water is 7.2–7.7, the color enhancer dosage is 1.2% of the fish's body weight. When the pH of the aquaculture water is 7.8–8.2, the amount of color enhancer fed is 0.30% of the fish's body weight; when the pH of the aquaculture water is 8.3–8.6, the amount of color enhancer fed is 0.80% of the fish's body weight; the amount of basic feed fed is 4% of the fish's body weight, and the components of the basic feed, by weight, include 35 parts white fish meal, 30 parts earthworm meal, 12 parts corn gluten meal, 10 parts soybean meal, 8 parts soybean oil, 5 parts choline chloride and 4 parts vitamin C;

[0076] (5) Water flow stimulation: The water body is kept at a flow rate of 0.12 m / s for 3 hours, 0.25 m / s for 2 hours, and 0.38 m / s for 1 hour every day, and the water flow is stopped at other times.

[0077] Comparative Example 5

[0078] The difference between this comparative example and Example 3 is that the irradiation time of the white light source and the total irradiation time of the red-blue-yellow light source are changed, while the other components and steps remain the same.

[0079] A breeding method for enhancing the coloration of the Oriental Star Spot includes the following steps:

[0080] (1) Construction of breeding ponds: Indoor breeding ponds are constructed, and the breeding ponds are disinfected with a mixture of bleaching powder and chlorine dioxide before stocking the fish fry.

[0081] (2) Stocking fry: The water temperature in the rearing pond should be 22-29℃, the salinity 25-32‰, and the dissolved oxygen above 5mg / L. Select healthy, disease-free, and injury-free grouper fry of 2.5-3cm and stock them in the rearing pond at a rate of 100-200 fry / m². 3 Before releasing the grouper fry, soak them in salt water for 10 minutes to disinfect them.

[0082] (3) Light induction: After the fish fry are released, they are irradiated with alternating white-red-blue-yellow light sources. The irradiation time of white light source is longer than the total irradiation time of red-blue-yellow light source. The irradiation time of white light source is 06:00-21:00 with an irradiation intensity of 120 lux. The irradiation time of red light source is 21:00-04:00 with an irradiation intensity of 600 lux. The irradiation time of blue light source is 04:00-05:00 with an irradiation intensity of 400 lux. The irradiation time of yellow light source is 05:00-06:00 with an irradiation intensity of 250 lux.

[0083] (4) Color enhancer administration: Administer simultaneously with the basic feed, three times daily at 09:00, 13:00, and 18:00. The color enhancer components, by weight, include 25 parts of Camellia chrysantha polysaccharide, 22 parts of Sargassum fusiforme polysaccharide, 15 parts of fucoxanthin, 10 parts of scallop peptide, 6 parts of silymarin, 2 parts of guanidinoacetic acid, and 2 parts of sodium butyrate. When the pH of the aquaculture water is 7.2–7.7, the color enhancer dosage is 1.2% of the fish's body weight. When the pH of the aquaculture water is 7.8–8.2, the amount of color enhancer fed is 0.30% of the fish's body weight; when the pH of the aquaculture water is 8.3–8.6, the amount of color enhancer fed is 0.80% of the fish's body weight; the amount of basic feed fed is 4% of the fish's body weight, and the components of the basic feed, by weight, include 35 parts white fish meal, 30 parts earthworm meal, 12 parts corn gluten meal, 10 parts soybean meal, 8 parts soybean oil, 5 parts choline chloride and 4 parts vitamin C;

[0084] (5) Water flow stimulation: The water body is kept at a flow rate of 0.12 m / s for 3 hours, 0.25 m / s for 2 hours, and 0.38 m / s for 1 hour every day, and the water flow is stopped at other times.

[0085] Comparative Example 6

[0086] The difference between this comparative example and Example 3 is that no color enhancer was fed, while the other components and steps remained unchanged.

[0087] A breeding method for enhancing the coloration of the Oriental Star Spot includes the following steps:

[0088] (1) Construction of breeding ponds: Indoor breeding ponds are constructed, and the breeding ponds are disinfected with a mixture of bleaching powder and chlorine dioxide before stocking the fish fry.

[0089] (2) Stocking fry: The water temperature in the rearing pond should be 22-29℃, the salinity 25-32‰, and the dissolved oxygen above 5mg / L. Select healthy, disease-free, and injury-free grouper fry of 2.5-3cm and stock them in the rearing pond at a rate of 100-200 fry / m². 3 Before releasing the grouper fry, soak them in salt water for 10 minutes to disinfect them.

[0090] (3) Light induction: After the fish fry are released, they are irradiated with alternating white-red-blue-yellow light sources. The irradiation time of white light source is the same as the total irradiation time of red-blue-yellow light source. The irradiation time of white light source is 06:00-18:00 with an irradiation intensity of 120 lux. The irradiation time of red light source is 18:00-02:00 with an irradiation intensity of 600 lux. The irradiation time of blue light source is 02:00-04:00 with an irradiation intensity of 400 lux. The irradiation time of yellow light source is 04:00-06:00 with an irradiation intensity of 250 lux.

[0091] (4) Basic feed feeding: Feed 3 times a day at 09:00, 13:00 and 18:00. The amount of basic feed is 4% of the fish's body weight. The components of the basic feed, by weight, include 35 parts white fish meal, 30 parts earthworm meal, 12 parts corn gluten meal, 10 parts soybean meal, 8 parts soybean oil, 5 parts choline chloride and 4 parts vitamin C.

[0092] (5) Water flow stimulation: The water body is kept at a flow rate of 0.12 m / s for 3 hours, 0.25 m / s for 2 hours, and 0.38 m / s for 1 hour every day, and the water flow is stopped at other times.

[0093] Comparative Example 7

[0094] The difference between this comparative example and Example 3 is that the colorant component does not include Camellia chrysanthemi polysaccharide and Sargassum fusiforme polysaccharide, while the other components and steps remain unchanged.

[0095] A breeding method for enhancing the coloration of the Oriental Star Spot includes the following steps:

[0096] (1) Construction of breeding ponds: Indoor breeding ponds are constructed, and the breeding ponds are disinfected with a mixture of bleaching powder and chlorine dioxide before stocking the fish fry.

[0097] (2) Stocking fry: The water temperature in the rearing pond should be 22-29℃, the salinity 25-32‰, and the dissolved oxygen above 5mg / L. Select healthy, disease-free, and injury-free grouper fry of 2.5-3cm and stock them in the rearing pond at a rate of 100-200 fry / m². 3 Before releasing the grouper fry, soak them in salt water for 10 minutes to disinfect them.

[0098] (3) Light induction: After the fish fry are released, they are irradiated with alternating white-red-blue-yellow light sources. The irradiation time of white light source is the same as the total irradiation time of red-blue-yellow light source. The irradiation time of white light source is 06:00-18:00 with an irradiation intensity of 120 lux. The irradiation time of red light source is 18:00-02:00 with an irradiation intensity of 600 lux. The irradiation time of blue light source is 02:00-04:00 with an irradiation intensity of 400 lux. The irradiation time of yellow light source is 04:00-06:00 with an irradiation intensity of 250 lux.

[0099] (4) Color enhancer administration: Administer simultaneously with the basic feed, three times daily at 09:00, 13:00, and 18:00. The color enhancer components, by weight, include 15 parts fucoxanthin, 10 parts scallop peptide, 6 parts silymarin, 2 parts guanidinoacetic acid, and 2 parts sodium butyrate. When the pH of the aquaculture water is 7.2–7.7, the color enhancer dosage is 1.2% of the fish's body weight. When the pH of the aquaculture water is… When the pH value of the aquaculture water is 7.8–8.2, the amount of color enhancer fed is 0.30% of the fish body weight; when the pH value of the aquaculture water is 8.3–8.6, the amount of color enhancer fed is 0.80% of the fish body weight; the amount of basic feed fed is 4% of the fish body weight, and the components of the basic feed, by weight, include 35 parts white fish meal, 30 parts earthworm meal, 12 parts corn gluten meal, 10 parts soybean meal, 8 parts soybean oil, 5 parts choline chloride and 4 parts vitamin C;

[0100] (5) Water flow stimulation: The water body is kept at a flow rate of 0.12 m / s for 3 hours, 0.25 m / s for 2 hours, and 0.38 m / s for 1 hour every day, and the water flow is stopped at other times.

[0101] Comparative Example 8

[0102] The difference between this comparative example and Example 3 is that the amount of color enhancer fed does not change with the pH value of the aquaculture water, while the other components and steps remain unchanged.

[0103] A breeding method for enhancing the coloration of the Oriental Star Spot includes the following steps:

[0104] (1) Construction of breeding ponds: Indoor breeding ponds are constructed, and the breeding ponds are disinfected with a mixture of bleaching powder and chlorine dioxide before stocking the fish fry.

[0105] (2) Stocking fry: The water temperature in the rearing pond should be 22-29℃, the salinity 25-32‰, and the dissolved oxygen above 5mg / L. Select healthy, disease-free, and injury-free grouper fry of 2.5-3cm and stock them in the rearing pond at a rate of 100-200 fry / m². 3 Before releasing the grouper fry, soak them in salt water for 10 minutes to disinfect them.

[0106] (3) Light induction: After the fish fry are released, they are irradiated with alternating white-red-blue-yellow light sources. The irradiation time of white light source is the same as the total irradiation time of red-blue-yellow light source. The irradiation time of white light source is 06:00-18:00 with an irradiation intensity of 120 lux. The irradiation time of red light source is 18:00-02:00 with an irradiation intensity of 600 lux. The irradiation time of blue light source is 02:00-04:00 with an irradiation intensity of 400 lux. The irradiation time of yellow light source is 04:00-06:00 with an irradiation intensity of 250 lux.

[0107] (4) Color enhancer feeding: Feed simultaneously with the basic feed, 3 times a day, at 09:00, 13:00 and 18:00. The components of the color enhancer, by weight, include 25 parts of Camellia chrysantha polysaccharide, 22 parts of Sargassum fusiforme polysaccharide, 15 parts of fucoxanthin, 10 parts of scallop peptide, 6 parts of silymarin, 2 parts of guanidinoacetic acid and 2 parts of sodium butyrate. The amount of color enhancer fed is 0.80% of the fish's body weight. The amount of basic feed fed is 4% of the fish's body weight. The components of the basic feed, by weight, include 35 parts of white fish meal, 30 parts of earthworm meal, 12 parts of corn gluten meal, 10 parts of soybean meal, 8 parts of soybean oil, 5 parts of choline chloride and 4 parts of vitamin C.

[0108] (5) Water flow stimulation: The water body is kept at a flow rate of 0.12 m / s for 3 hours, 0.25 m / s for 2 hours, and 0.38 m / s for 1 hour every day, and the water flow is stopped at other times.

[0109] Comparative Example 9

[0110] The difference between this comparative example and Example 3 is that there is no water flow stimulation, while the other components and steps remain unchanged.

[0111] A breeding method for enhancing the coloration of the Oriental Star Spot includes the following steps:

[0112] (1) Construction of breeding ponds: Indoor breeding ponds are constructed, and the breeding ponds are disinfected with a mixture of bleaching powder and chlorine dioxide before stocking the fish fry.

[0113] (2) Stocking fry: The water temperature in the rearing pond should be 22-29℃, the salinity 25-32‰, and the dissolved oxygen above 5mg / L. Select healthy, disease-free, and injury-free grouper fry of 2.5-3cm and stock them in the rearing pond at a rate of 100-200 fry / m². 3 Before releasing the grouper fry, soak them in salt water for 10 minutes to disinfect them.

[0114] (3) Light induction: After the fish fry are released, they are irradiated with alternating white-red-blue-yellow light sources. The irradiation time of white light source is the same as the total irradiation time of red-blue-yellow light source. The irradiation time of white light source is 06:00-18:00 with an irradiation intensity of 120 lux. The irradiation time of red light source is 18:00-02:00 with an irradiation intensity of 600 lux. The irradiation time of blue light source is 02:00-04:00 with an irradiation intensity of 400 lux. The irradiation time of yellow light source is 04:00-06:00 with an irradiation intensity of 250 lux.

[0115] (4) Color enhancer administration: Administer simultaneously with the basic feed, three times daily at 09:00, 13:00, and 18:00. The color enhancer components, by weight, include 25 parts of Camellia chrysantha polysaccharide, 22 parts of Sargassum fusiforme polysaccharide, 15 parts of fucoxanthin, 10 parts of scallop peptide, 6 parts of silymarin, 2 parts of guanidinoacetic acid, and 2 parts of sodium butyrate. When the pH of the aquaculture water is 7.2–7.7, the color enhancer dosage is 1.2% of the fish's body weight. When the pH of the aquaculture water is 7.8–8.2, the amount of color enhancer fed is 0.30% of the fish's body weight; when the pH of the aquaculture water is 8.3–8.6, the amount of color enhancer fed is 0.80% of the fish's body weight; the amount of basic feed fed is 4% of the fish's body weight, and the components of the basic feed, by weight, include 35 parts white fish meal, 30 parts earthworm meal, 12 parts corn gluten meal, 10 parts soybean meal, 8 parts soybean oil, 5 parts choline chloride and 4 parts vitamin C;

[0116] Comparative Example 10

[0117] The difference between this comparative example and Example 3 is that there was no repeated water flow stimulation, while the other components and steps remained unchanged.

[0118] A breeding method for enhancing the coloration of the Oriental Star Spot includes the following steps:

[0119] (1) Construction of breeding ponds: Indoor breeding ponds are constructed, and the breeding ponds are disinfected with a mixture of bleaching powder and chlorine dioxide before stocking the fish fry.

[0120] (2) Stocking fry: The water temperature in the rearing pond should be 22-29℃, the salinity 25-32‰, and the dissolved oxygen above 5mg / L. Select healthy, disease-free, and injury-free grouper fry of 2.5-3cm and stock them in the rearing pond at a rate of 100-200 fry / m². 3 Before releasing the grouper fry, soak them in salt water for 10 minutes to disinfect them.

[0121] (3) Light induction: After the fish fry are released, they are irradiated with alternating white-red-blue-yellow light sources. The irradiation time of white light source is the same as the total irradiation time of red-blue-yellow light source. The irradiation time of white light source is 06:00-18:00 with an irradiation intensity of 120 lux. The irradiation time of red light source is 18:00-02:00 with an irradiation intensity of 600 lux. The irradiation time of blue light source is 02:00-04:00 with an irradiation intensity of 400 lux. The irradiation time of yellow light source is 04:00-06:00 with an irradiation intensity of 250 lux.

[0122] (4) Color enhancer administration: Administer simultaneously with the basic feed, three times daily at 09:00, 13:00, and 18:00. The color enhancer components, by weight, include 25 parts of Camellia chrysantha polysaccharide, 22 parts of Sargassum fusiforme polysaccharide, 15 parts of fucoxanthin, 10 parts of scallop peptide, 6 parts of silymarin, 2 parts of guanidinoacetic acid, and 2 parts of sodium butyrate. When the pH of the aquaculture water is 7.2–7.7, the color enhancer dosage is 1.2% of the fish's body weight. When the pH of the aquaculture water is 7.8–8.2, the amount of color enhancer fed is 0.30% of the fish's body weight; when the pH of the aquaculture water is 8.3–8.6, the amount of color enhancer fed is 0.80% of the fish's body weight; the amount of basic feed fed is 4% of the fish's body weight, and the components of the basic feed, by weight, include 35 parts white fish meal, 30 parts earthworm meal, 12 parts corn gluten meal, 10 parts soybean meal, 8 parts soybean oil, 5 parts choline chloride and 4 parts vitamin C;

[0123] (5) Water flow stimulation: The water body is kept at a flow rate of 0.12 m / s for 3 hours a day, and the flow is stopped for the rest of the time.

[0124] Experimental Example: Measurement of Experimental Indicators

[0125] 1.1 Test Object

[0126] The grouper raised for 60 days using the methods described in Examples 1-3 and Comparative Examples 1-10 were used to set up a control group (application number 202210800228.3, patent subject: an indoor seedling raising method for grouper).

[0127] 1.2 Test Methods

[0128] 1.2.1 Growth Indicators

[0129] Growth rate (MG) = (W) t -W0) / W0×100%, where W t W0 is the average body mass of the fish at the end of the feeding cycle (g), and W0 is the average body mass of the fish at the beginning of the feeding cycle (g); Feed conversion ratio (FCR) = F / (W t-W0), where F is the food intake. Experimental data are expressed as mean ± standard deviation and statistically analyzed using SPSS 19.0 software.

[0130] 1.2.2 Body Color Indicators

[0131] The brightness (L) of the abdomen of the Oriental Star Spot was measured using a CR-400 colorimeter. ＊ ), red-green value (a ＊ ) and yellow-blue value (b ＊ L ＊ The higher the value, the brighter the body color; a ＊ A positive value indicates a reddish tint, while a negative value indicates a greenish tint; b ＊ Positive values ​​indicate a yellowish tint, while negative values ​​indicate a bluish tint. When measuring, first use absorbent paper to dry the surface of the fish, then place the colorimeter probe firmly against the area of ​​the fish to be tested to read the results.

[0132] 1.3 Experimental Results

[0133] 1.3.1 The growth rate and feed conversion ratio results are shown in Table 1, and the body color index is shown in Table 2;

[0134] Table 1 Growth indicators after 60 days of culture

[0135]

[0136]

[0137] Table 2 Abdominal body color indicators after 60 days of rearing

[0138]

[0139] Tables 1 and 2 show that the synergistic effect of light induction, color enhancer feeding, and water flow stimulation significantly improves the growth rate and feed conversion ratio of grouper, while also enhancing body color, making the grouper more vibrant and significantly increasing its economic value. Example 3 showed the best results, outperforming the control group. Comparing Example 3 with Comparative Examples 1-4, it is evident that alternating white-red-blue-yellow irradiation is more effective than a single light source in enhancing grouper body color. Alternating light qualities stimulates different photoreceptors, activating multiple gene pathways related to pigment synthesis, promoting comprehensive expression of cytochromes. The dynamic light environment enhances the grouper's sensitivity to photoperiod, regulates the secretion of hormones related to body color, and thus promotes the differentiation and migration of pigment cells, which is beneficial for enhancing grouper body color. However, it is crucial to carefully control the irradiation time of each light source to avoid activating only some metabolic pathways, which may inhibit the production of other pigments. Comparing Example 3 with Comparative Examples 6-8, it is evident that the color enhancer... The synergistic combination of active ingredients allows the color enhancer not only to improve body color but also to increase the proportion of feed converted into grouper meat, further improving the quality of the fish meat. In particular, the polysaccharides of Camellia chrysantha and Sargassum fusiforme are being used for the first time in the direction of improving fish body color. As can be seen from the comparison between Example 3 and Comparative Examples 9 and 10, changing the water flow speed multiple times and using water flow stimulation can stimulate the movement frequency of grouper, accelerate the metabolic rate, and promote more efficient synthesis and distribution of pigment cells. At the same time, dynamic water flow can stimulate the body color regulation mechanism of grouper to adapt to water flow changes, which can prevent pigment cells from entering an "inert" state due to lack of external stimulation, thereby maintaining or enhancing body color.

[0140] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A breeding method for enhancing the body color of the Oriental Star Spot, characterized in that, Includes the following steps: (1) Illumination induction: Alternating white-red-blue-yellow light sources were used for illumination; (2) Color enhancer feeding: Feed simultaneously with the basic feed. The components of the color enhancer include Camellia chrysantha polysaccharide, Sargassum fusiforme polysaccharide, fucoxanthin, scallop peptide, silymarin, guanidinoacetic acid and sodium butyrate. When the pH of the aquaculture water is 7.2-7.7, the amount of color enhancer fed is 0.8-1.5% of the fish body weight; when the pH of the aquaculture water is 7.8-8.2, the amount of color enhancer fed is 0.15-0.35% of the fish body weight; when the pH of the aquaculture water is 8.3-8.6, the amount of color enhancer fed is 0.50-1.0% of the fish body weight. (3) Water flow stimulation: The water body is kept at a flow rate of 0.10-0.15 m / s for 2-4 hours, at a flow rate of 0.20-0.30 m / s for 1-3 hours, at a flow rate of 0.35-0.40 m / s for 0.5-1.5 hours, and the flow is stopped at other times.

2. The breeding method for enhancing the body color of the Oriental Star Spot according to claim 1, characterized in that, The illumination time for white light source in step (1) is the same as the total illumination time for red-blue-yellow light source.

3. The breeding method for enhancing the body color of the Oriental Star Spot according to claim 1, characterized in that, In step (1), the illumination time for white light source is 06:00-18:00, the illumination time for red light source is 18:00-02:00, the illumination time for blue light source is 02:00-04:00, and the illumination time for yellow light source is 04:00-06:

00.

4. The breeding method for enhancing the body color of the Oriental Star Spot according to claim 1, characterized in that, In step (1), the illumination intensity of white light source is 100-150 lux, the illumination intensity of red light source is 500-1000 lux, the illumination intensity of blue light source is 300-500 lux, and the illumination intensity of yellow light source is 200-300 lux.

5. The breeding method for enhancing the body color of the Oriental Star Spot according to claim 1, characterized in that, The components of the basic feed described in step (2) by weight include 30-40 parts white fish meal, 20-35 parts earthworm meal, 10-15 parts corn gluten meal, 7-12 parts soybean meal, 5-10 parts soybean oil, 2-7 parts choline chloride and 1-6 parts vitamin C.

6. The breeding method for enhancing the body color of the Oriental Star Spot according to claim 1, characterized in that, The amount of basic feed mentioned in step (2) is 3 to 5% of the fish's body weight.

7. The breeding method for enhancing the body color of the Oriental Star Spot according to claim 1, characterized in that, The color enhancer in step (2) comprises, by weight, 20-30 parts of Camellia chrysantha polysaccharide, 20-25 parts of Sargassum fusiforme polysaccharide, 10-18 parts of fucoxanthin, 8-12 parts of scallop peptide, 4-10 parts of silymarin, 1-3 parts of guanidinoacetic acid and 1-3 parts of sodium butyrate.

8. The breeding method for enhancing the body color of the Oriental Star Spot according to claim 7, characterized in that, The color enhancer in step (2) comprises, by weight, 25 parts of Camellia chrysantha polysaccharide, 22 parts of Sargassum fusiforme polysaccharide, 15 parts of fucoxanthin, 10 parts of scallop peptide, 6 parts of silymarin, 2 parts of guanidinoacetic acid and 2 parts of sodium butyrate.

9. The breeding method for enhancing the body color of the Oriental Star Spot according to claim 1, characterized in that, In step (2), when the pH of the aquaculture water is 7.2 to 7.7, the amount of color enhancer fed is 1.2% of the fish's body weight; when the pH of the aquaculture water is 7.8 to 8.2, the amount of color enhancer fed is 0.30% of the fish's body weight; and when the pH of the aquaculture water is 8.3 to 8.6, the amount of color enhancer fed is 0.80% of the fish's body weight.

10. The breeding method for enhancing the body color of the Oriental Star Spot according to claim 1, characterized in that, In step (3), the water body is kept at a flow rate of 0.12 m / s for 3 hours, at a flow rate of 0.25 m / s for 2 hours, and at a flow rate of 0.38 m / s for 1 hour each day, and the flow is stopped for the rest of the time.

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