Blood test sample pollution-free preservation method

Abstract

The invention discloses a pollution-free preservation method for a blood test sample, and belongs to the technical field of medical examination. The method specifically comprises the following steps: S1, collection and initial treatment of a sample: collecting a blood test sample, and carrying out primary treatment on the collected blood test sample; s2, treatment of the storage container: performing anti-pollution treatment on the storage container of the blood test sample, and dynamically monitoring the storage environment of the storage container. The inner wall of the sterile blood collection tube is coated with polytetrafluoroethylene, heparin sodium and EDTA composite anticoagulant are used, blood coagulation and microbial adhesion can be prevented, meanwhile, the hemolysis risk is reduced, and leakage can be avoided in the transportation process by adopting vacuum aluminum film sealing after rubber plug sealing. Silver nanoparticles and a zinc oxide coating are sprayed on the inner wall of the storage container, microorganism proliferation can be inhibited, the upper layer in the storage container is filled with a nitrogen layer to isolate oxygen, a lower-layer sample area maintains low positive pressure to prevent leakage, and the local temperature gradient can be effectively eliminated through distributed cooling storage.

Description

Technical Field

[0001] The present invention relates to the technical field of medical testing, and particularly to a method for preserving blood test samples without contamination. Background Art

[0002] A blood test sample refers to a small amount of blood collected from the human body or an animal body for medical testing, disease diagnosis, scientific research, or other analytical purposes. It is one of the most common biological samples in medical treatment and scientific research. By analyzing the compositional changes in the blood test sample, it provides key information for doctors and researchers and is an indispensable diagnostic tool in modern medicine. To ensure the accuracy of the analysis results of blood test samples, it is necessary to ensure that the blood test samples are not contaminated during storage. Most of the existing methods for preserving blood test samples without contamination only focus on how to ensure that the blood test samples are not contaminated, but lack the detection and early warning of blood test samples, resulting in the inability to timely detect the contamination risk during the storage process of blood test samples and take measures to avoid the contamination of blood test samples. Summary of the Invention

[0003] Object of the Invention: The object of the present invention is to provide a method for preserving blood test samples without contamination, which can solve the problem that the contamination risk of blood test samples cannot be timely detected and processed during the storage process to avoid the contamination of blood test samples.

[0004] Technical Solution: To solve the above technical problems, according to one aspect of the present invention, more specifically, a method for preserving blood test samples without contamination, which specifically includes the following steps:

[0005] S1. Collection and Initial Processing of Samples: Collect the blood test samples and perform primary processing on the collected blood test samples.

[0006] S2. Processing of the Preservation Container: Perform anti-contamination processing on the preservation container of the blood test samples and dynamically monitor the preservation environment of the preservation container.

[0007] S3. Dynamic Regulation of the Preservation Environment: Dynamically regulate the preservation environment of the blood test samples to ensure that the preservation environment meets the preservation requirements of the blood test samples.

[0008] S4. Real-time Data Monitoring and Collection: Real-time monitor and collect various data of the preservation environment of the blood test samples and various data of the blood test samples.

[0009] S5. Analysis and Processing of the Collected Data: Analyze and process various data of the preservation environment of the collected blood test samples and various data of the blood test samples.

[0010] S6. Comparison and Early Warning of the Analysis and Processing Results: Compare the analysis and processing results with the threshold of the preset analysis and processing results, and issue a warning prompt for the contamination risk of the blood test samples according to the comparison results.

[0011] Furthermore, in the step S1, when collecting the sample, the inner wall of the sterile blood collection tube is coated with polytetrafluoroethylene, a heparin sodium and EDTA composite anticoagulant is used, and after being sealed with a rubber stopper, it is sealed with a vacuum aluminum film.

[0012] Furthermore, in the step S2, when performing anti-pollution treatment on the storage container of the blood test sample, the inner wall of the storage container is sprayed with a silver nanoparticle and zinc oxide coating, and a nitrogen layer is filled in the upper layer inside the storage container, and a low positive pressure is maintained in the lower sample area.

[0013] Furthermore, in the step S2, the dynamic monitoring of the storage environment of the storage container includes dynamic monitoring of, but not limited to, temperature, humidity, vibration, and light.

[0014] Furthermore, in the step S3, the regulation method for dynamically regulating the storage environment includes distributed cooling storage of the stored blood test samples, maintaining the storage temperature at 3.5 - 4.5 °C, and at the same time maintaining the environmental pH value at 7.2 - 7.6.

[0015] Furthermore, in the step S4, when performing real-time data collection, it includes the temperature and humidity of the storage environment of the blood test sample, as well as the cell membrane integrity data, microbial detection data, and oxidative stress detection data of the blood test sample.

[0016] Furthermore, in the step S5, when analyzing and processing the collected data, the temperature and humidity of the storage environment of the blood test sample, as well as the cell membrane integrity data, microbial detection data, and oxidative stress detection data of the blood test sample are obtained, and the above data are analyzed and processed to obtain the pollution risk index of each blood test sample. Thus, whether there is a risk of contamination of the corresponding blood test sample is determined through the pollution risk index of each blood test sample. When the pollution risk index of the blood test sample is larger, it indicates that the risk of contamination of the blood test sample is higher. When the pollution risk index of the blood test sample is smaller, it indicates that the risk of contamination of the blood test sample is lower.

[0017] Furthermore, in the step S6, when comparing the analysis and processing results with the threshold of the preset analysis and processing results, the pollution risk index of each blood test sample is compared with the threshold of the preset pollution risk index of the blood test sample. When the pollution risk index of the blood test sample is equal to or greater than the preset pollution risk index of the blood test sample, a warning notice of the pollution risk of the blood test sample is sent to relevant personnel. When the pollution risk index of the blood test sample is less than the preset pollution risk index of the blood test sample, a warning notice of the pollution risk of the blood test sample is not sent to relevant personnel.

[0018] Beneficial effects: The inner wall of the sterile blood collection tube is coated with polytetrafluoroethylene, and a composite anticoagulant of heparin sodium and EDTA is used, which can prevent blood coagulation and microbial adhesion, and at the same time reduce the risk of hemolysis. After being sealed with a rubber stopper, it is sealed with a vacuum aluminum film, which can avoid leakage during transportation. Spraying silver nanoparticles and zinc oxide coatings on the inner wall of the storage container can inhibit the proliferation of microorganisms, and a nitrogen layer is filled in the upper layer inside the storage container to isolate oxygen, and a low positive pressure is maintained in the lower sample area to prevent leakage. Through distributed cooling storage, local temperature gradients can be effectively eliminated. By obtaining the temperature and humidity of the storage environment of the blood test sample, as well as the cell membrane integrity data, microbial detection data, and oxidative stress detection data of the blood test sample, and analyzing and processing the above data to obtain the contamination risk index of each blood test sample, so as to determine whether there is a risk of contamination for the corresponding blood test sample through the contamination risk index of each blood test sample. When the contamination risk index of the blood test sample is larger, it indicates that the risk of contamination of the blood test sample is higher. When the contamination risk index of the blood test sample is smaller, it indicates that the risk of contamination of the blood test sample is lower. Comparing the contamination risk index of each blood test sample with the threshold value of the preset contamination risk index of the blood test sample, when the contamination risk index of the blood test sample is equal to or greater than the preset contamination risk index of the blood test sample, a warning notice of the contamination risk of the blood test sample is sent to relevant personnel. When the contamination risk index of the blood test sample is smaller than the preset contamination risk index of the blood test sample, a warning notice of the contamination risk of the blood test sample is not sent to relevant personnel. Description of the Drawings

[0019] Figure 1 It is a schematic diagram of the method flow. Detailed Implementation Modes

[0020] To make the technical solutions of the present invention clearer, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0021] Example 1

[0022] First step, collection and initial processing of the sample: Collect the blood test sample, and perform primary processing on the collected blood test sample. The inner wall of the sterile blood collection tube is coated with polytetrafluoroethylene, and a composite anticoagulant of heparin sodium and EDTA is used, which can prevent blood coagulation and microbial adhesion, and at the same time reduce the risk of hemolysis. After being sealed with a rubber stopper, it is sealed with a vacuum aluminum film, which can avoid leakage during transportation.

[0023] Step 2, Treatment of the storage container: Perform anti-pollution treatment on the storage container for the blood test sample. When performing anti-pollution treatment on the storage container for the blood test sample, spray silver nanoparticles and zinc oxide coating on the inner wall of the storage container, which can inhibit the proliferation of microorganisms. And fill a nitrogen layer in the upper layer inside the storage container, and maintain a low positive pressure in the lower sample area to prevent leakage. Also, dynamically monitor the storage environment of the storage container, including but not limited to temperature, humidity, vibration, and light.

[0024] Step 3, Dynamic regulation of the storage environment: Dynamically regulate the storage environment of the blood test sample to ensure that the storage environment meets the storage requirements of the blood test sample. The regulation methods for dynamically regulating the storage environment include distributed cooling storage of the stored blood test sample, which can effectively eliminate local temperature gradients and maintain the storage temperature at 3.5 - 4.5 °C, while maintaining the pH value of the storage environment at 7.2 - 7.6.

[0025] Step 4, Real-time data monitoring and collection: Real-time monitor and collect various data of the blood test sample storage environment and various data of the blood test sample, including the temperature and humidity of the blood test sample storage environment, as well as the collection of data on the cell membrane integrity, microbial detection, and oxidative stress detection of the blood test sample.

[0026] Step 5, Analysis and processing of the collected data: Analyze and process various data of the blood test sample storage environment and various data of the blood test sample collected. When analyzing and processing the collected data, obtain the temperature and humidity of the blood test sample storage environment, as well as the data on the cell membrane integrity, microbial detection, and oxidative stress detection of the blood test sample, and analyze and process the above data to obtain the pollution risk index of each blood test sample. Thus, determine whether there is a risk of contamination for the corresponding blood test sample based on the pollution risk index of each blood test sample. When the pollution risk index of the blood test sample is larger, it indicates that the risk of contamination of the blood test sample is higher. When the pollution risk index of the blood test sample is smaller, it indicates that the risk of contamination of the blood test sample is lower.

[0027] Step 6, Comparison of the analysis and processing results and early warning: Compare the analysis and processing results with the threshold of the preset analysis and processing results, and issue a warning prompt for the risk of contamination of the blood test sample according to the comparison result. When comparing the analysis and processing results with the threshold of the preset analysis and processing results, compare the pollution risk index of each blood test sample with the threshold of the preset pollution risk index of the blood test sample. When the pollution risk index of the blood test sample is equal to or greater than the preset pollution risk index of the blood test sample, issue a warning notice of the risk of contamination of the blood test sample to relevant personnel. When the pollution risk index of the blood test sample is less than the preset pollution risk index of the blood test sample, do not issue a warning notice of the risk of contamination of the blood test sample to relevant personnel.

[0028] The above-described embodiments merely represent several implementation manners of the present invention. The description thereof is relatively specific and detailed, but it should not be construed as a limitation to the scope of the patent for the present invention. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, several modifications and improvements can still be made, and these all fall within the protection scope of the present invention. Therefore, the protection scope of the patent for the present invention shall be subject to the appended claims.

Claims

1. A method for preserving blood test samples without contamination, characterized in that: The specific steps include: S1. Sample collection and initial processing: Collect blood test samples and perform primary processing on the collected blood test samples; S2. Treatment of storage containers: Anti-contamination treatment is performed on the storage containers of the blood test samples, and the storage environment of the storage containers is dynamically monitored; S3. Dynamic regulation of storage environment: Dynamically regulate the storage environment of blood test samples to ensure that the storage environment meets the storage needs of blood test samples; S4. Real-time data monitoring and collection: real-time monitoring and collection of various data of the blood test sample storage environment and various data of the blood test samples; S5, analyzing and processing collected data: analyzing and processing various data of the storage environment of the collected blood test samples and various data of the blood test samples; S6. Comparison and early warning of analysis and processing results: Compare the analysis and processing results with the preset analysis and processing result threshold, and issue an early warning prompt that the blood test sample has a risk of contamination based on the comparison result.

2. The method for non-contamination storage of blood test samples according to claim 1, characterized in that: In step S1, when collecting samples, the inner wall of the sterile blood collection tube is coated with polytetrafluoroethylene, and a composite anticoagulant of sodium heparin and EDTA is used. The tube is sealed with a rubber stopper and then with a vacuum aluminum film.

3. The method for non-contamination storage of blood test samples according to claim 1, characterized in that: In step S2, when the storage container of the blood test sample is subjected to anti-pollution treatment, silver nanoparticles and zinc oxide coating are sprayed on the inner wall of the storage container, and a nitrogen layer is filled in the upper layer of the storage container, and a low positive pressure is maintained in the lower sample area.

4. The method for non-contamination storage of blood test samples according to claim 1, characterized in that: In the step S2, the storage environment of the storage container is dynamically monitored, including but not limited to temperature, humidity, vibration, and light.

5. The method for non-contamination storage of blood test samples according to claim 1, characterized in that: In step S3, the dynamic control method of the storage environment includes distributed cooling of the stored blood test samples, maintaining the storage temperature at 3.5-4.5° C., and maintaining the environmental pH value at 7.2-7.

6.

6. The method for non-contamination storage of blood test samples according to claim 1, characterized in that: In step S4, when real-time data collection is performed, the data includes the temperature and humidity of the blood test sample storage environment, as well as the cell membrane integrity data of the blood test sample, the microbial detection data of the blood test sample, and the oxidative stress detection data of the blood test sample.

7. The method for non-contamination storage of blood test samples according to claim 1, characterized in that: In step S5, when analyzing and processing the collected data, the temperature and humidity of the blood test sample storage environment, the cell membrane integrity data of the blood test sample, the microbial detection data of the blood test sample, and the oxidative stress detection data of the blood test sample are obtained, and the above data are analyzed and processed to obtain the contamination risk index of each blood test sample, so as to determine whether the corresponding blood test sample has a contamination risk through the contamination risk index of each blood test sample. The larger the contamination risk index of the blood test sample, the higher the risk of contamination of the blood test sample, and the smaller the contamination risk index of the blood test sample, the lower the risk of contamination of the blood test sample.

8. The method for non-contamination storage of blood test samples according to claim 1, characterized in that: In step S6, when comparing the analysis and processing result with the preset analysis and processing result threshold, the contamination risk index of each blood test sample will be compared with the preset blood test sample contamination risk index threshold. When the contamination risk index of the blood test sample is equal to or greater than the preset blood test sample contamination risk index, an early warning notification of the contamination risk of the blood test sample is issued to notify relevant personnel; when the contamination risk index of the blood test sample is less than the preset blood test sample contamination risk index, no early warning notification of the contamination risk of the blood test sample is issued to notify relevant personnel.

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