Traditional Chinese medicine composition for preventing or treating infectious mononuclear hyperplasia

Abstract

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for preventing or treating infectious mononuclear hyperplasia and a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the following raw material medicines: astragalus membranaceus, codonopsis pilosula, rhizoma dryopteris crassirhizomae, trollflower, semen plantaginis, mint, dandelion and liquorice. The traditional Chinese medicine composition has the effects of tonifying qi and lifting yang, nourishing blood and promoting salivation, and clearing away heat and toxic materials. Experimental results show that the traditional Chinese medicine composition can remove Epstein-Barr virus, further thoroughly eliminates the symptoms of weakness, fever, myalgia, lymphadenectasis and hepatometasis of patients, and finally realizes the curative treatment of infectious mononuclear hyperplasia, so that the traditional Chinese medicine composition has better development and utilization prospects.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition for preventing or treating infectious mononucleosis, a preparation method thereof, and uses thereof. Background Art

[0002] Infectious mononucleosis (IM) is an acute self-limiting infectious disease, and about 90% of patients are caused by Epstein-Barr virus (EBV). This disease is common in preschool children aged 3 - 7 years. Clinically, typical triad symptoms of infectious mononucleosis can be seen: irregular fever, pharyngitis, and lymph node enlargement, which may be combined with hepatosplenomegaly, rash, and an increase in atypical lymphocytes (ALY) in peripheral blood. The clinical symptoms of this disease are atypical or diverse, and the sequence of symptom appearance is irregular, making it easy to be misdiagnosed or missed diagnosed as upper respiratory tract infection, tonsillitis, lymphadenitis. Some patients may develop into hemophagocytic syndrome, myocarditis, meningitis, splenic rupture, etc., which can endanger life. Western medicine mainly uses antiviral drugs to treat this disease, but its curative effect is not exact, and there are adverse reactions such as fewer white blood cells and platelets, hematuria, infection, arrhythmia, ataxia, coma, etc.

[0003] In traditional Chinese medicine, there is no specific disease name for infectious mononucleosis, but there are records and descriptions similar to this disease. According to its clinical symptoms, IM can be classified into infectious disease categories such as "sore throat obstruction", "phlegm nodes", "scrofula", "warm toxin", etc. For example, "On Warm Toxin Diseases" records: "When winter warm, spring warm, damp warm, and damp epidemic diseases are prevalent, a warm toxin symptom is often mixed in." "Treatise on the Causes and Symptoms of Various Diseases" states: "Wind-heat toxin invades the throat and between the forehead and cheeks, and combines with qi and blood to form swelling and pain." "Treatise on Pestilence Rashes" says: "The throat is the passage for breathing, and when the poisonous fire fumigates, it causes swelling and pain." "Spiritual Pivot · Cold and Heat" says: "Cold and heat scrofula in the lower legs and armpits are all due to the poisonous qi of rat fistula cold and heat."

[0004] Currently, in clinical practice, the treatment principle is mainly clearing heat and detoxifying, and classical prescriptions such as Yinqiao Powder, Qingying Decoction, Qingwen Baidu Decoction, Dayuan Decoction, Puji Xiaodu Yin, Qinghao Biejia Decoction, Ganlu Xiaodu Dan, Daiha Powder, Gegen Jijie Decoction, Dachaihu Decoction, Sanyiren Decoction, Sangju Decoction, Baihu Decoction, and Angong Niuhuang Pills, which mainly clear heat and detoxify, are used to prevent and treat heat toxin diseases such as infectious mononucleosis. However, these treatment methods may generally have drawbacks such as incorrect prescription composition and unclear treatment effect.

[0005] In view of this, to meet the clinical treatment needs, the inventor's team has developed a traditional Chinese medicine composition for preventing or treating infectious mononucleosis with good curative effect, no toxic and side effects, easily available raw materials, and low cost. Summary of the Invention

[0006] The object of the present invention is to overcome the deficiencies existing in the prior art and provide a traditional Chinese medicine composition for preventing or treating infectious mononucleosis, its preparation method and uses.

[0007] Specifically, the present invention is achieved through the following technical solutions in several aspects:

[0008] In the first aspect, the present invention provides a traditional Chinese medicine composition for preventing or treating infectious mononucleosis, and the traditional Chinese medicine composition is made from the following raw materials by weight: 10 - 50 parts of Astragalus membranaceus, 10 - 50 parts of Codonopsis pilosula, 5 - 40 parts of Dryopteris crassirhizoma, 5 - 40 parts of Trollius chinensis, 5 - 40 parts of Plantago asiatica, 1 - 30 parts of Mentha haplocalyx, 1 - 30 parts of Taraxacum mongolicum, 1 - 20 parts of Glycyrrhiza uralensis.

[0009] As an optional way, in the above traditional Chinese medicine composition, the traditional Chinese medicine composition is made from the following raw materials by weight: 10 - 40 parts of Astragalus membranaceus, 10 - 40 parts of Codonopsis pilosula, 10 - 30 parts of Dryopteris crassirhizoma, 10 - 30 parts of Trollius chinensis, 10 - 30 parts of Plantago asiatica, 1 - 20 parts of Mentha haplocalyx, 1 - 20 parts of Taraxacum mongolicum, 1 - 15 parts of Glycyrrhiza uralensis.

[0010] As an optional way, in the above traditional Chinese medicine composition, the traditional Chinese medicine composition is made from the following raw materials by weight: 30 parts of Astragalus membranaceus, 30 parts of Codonopsis pilosula, 20 parts of Dryopteris crassirhizoma, 20 parts of Trollius chinensis, 20 parts of Plantago asiatica, 15 parts of Mentha haplocalyx, 15 parts of Taraxacum mongolicum, 10 parts of Glycyrrhiza uralensis.

[0011] As an optional way, in the above traditional Chinese medicine composition, the traditional Chinese medicine composition has the effects of invigorating qi and ascending yang, nourishing blood and promoting fluid production, clearing heat and detoxifying.

[0012] In the second aspect, the present invention provides a preparation method of the traditional Chinese medicine composition described in the first aspect above, and the preparation method includes the following steps: pulverize Astragalus membranaceus, Codonopsis pilosula, Dryopteris crassirhizoma, Trollius chinensis, Plantago asiatica, Mentha haplocalyx, Taraxacum mongolicum, Glycyrrhiza uralensis, add water for decoction, mix the filtrates, and obtain a concentrated solution through concentration.

[0013] As an optional way, in the above preparation method, the number of decoction times is two. After the first decoction for 1 - 3 hours, filter to obtain the first filtrate. Then add water to the residue for decoction for 1 - 3 hours, and filter to obtain the second filtrate. Combine the first filtrate and the second filtrate.

[0014] As an optional way, in the above preparation method, the material - liquid ratio for both the first decoction and the second decoction is 1:4, the decoction time for both the first decoction and the second decoction is 1 hour, and the water used for both the first decoction and the second decoction is distilled water.

[0015] Preferably, the concentration is vacuum concentration.

[0016] In a third aspect, the present invention provides the use of the traditional Chinese medicine composition described in the first aspect above or the traditional Chinese medicine composition prepared by using the preparation method described in the second aspect above in the preparation of a drug for preventing or treating infectious mononucleosis.

[0017] As an optional mode, in the above use, the infectious mononucleosis is childhood infectious mononucleosis.

[0018] In a fourth aspect, the present invention provides the use of the traditional Chinese medicine composition described in the first aspect above or the traditional Chinese medicine composition prepared by using the preparation method described in the second aspect above in the preparation of a drug for clearing Epstein - Barr virus (EB virus).

[0019] As an optional mode, in the above use, the dosage form of the drug is capsule, granule, tablet, liquid preparation, dripping pill, spray, rectal administration preparation or injection.

[0020] Preferably, the tablet is an effervescent tablet.

[0021] The present invention has the following beneficial effects compared with the prior art:

[0022] (1) The traditional Chinese medicine composition of the present invention has the effects of invigorating qi to lift yang, nourishing blood to promote fluid production, and clearing heat and detoxifying. The experimental results show that the traditional Chinese medicine composition of the present invention can clear Epstein - Barr virus, and then completely eliminate the symptoms of fatigue, fever, myalgia, lymph node enlargement, and hepatosplenomegaly in patients, and finally achieve a curative treatment for infectious mononucleosis. Therefore, it has good development and utilization prospects.

[0023] (2) On the basis of the traditional Chinese medicine formula, the traditional Chinese medicine composition of the present invention utilizes modern preparation techniques and is deeply optimized. The preparation method is simple, and the traditional Chinese medicine composition of the present invention can be made into various dosage forms such as capsules, granules, tablets, liquid preparations, dripping pills, sprays, rectal administration preparations or injections. Detailed implementation manners

[0024] The traditional Chinese medicine composition of the present invention has the effects of invigorating qi to lift yang, nourishing blood to promote fluid production, and clearing heat and detoxifying. Astragalus membranaceus and Codonopsis pilosula in the formula are monarch drugs; Dryopteris crassirhizoma, Trollius chinensis, and Plantago asiatica are ministerial drugs; Mentha haplocalyx and Taraxacum mongolicum are adjuvant drugs; Glycyrrhiza uralensis is the guiding drug.

[0025] The efficacy and main treatment of each medicinal flavor in the traditional Chinese medicine composition of the present invention are as follows:

[0026] "Astragalus Root" is the root of Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao and Astragalus membranaceus (Fisch.) Bge. of the Leguminosae family. It is slightly warm in nature and sweet in taste. It belongs to the Spleen Meridian and Lung Meridian. It can replenish qi and raise yang, secure the exterior and stop sweating, promote diuresis and alleviate edema, generate body fluid and nourish blood, promote qi circulation to relieve pain, expel pus by supporting healthy qi, and promote granulation and muscle growth. It belongs to the qi-tonifying herbs under the classification of tonifying deficiency herbs. It is used to treat shortness of breath, palpitation, fatigue, collapse, spontaneous sweating, night sweating, general edema due to deficiency, chronic nephritis, chronic diarrhea, rectal prolapse, uterine prolapse, difficulty in ulceration of carbuncles and sores, non-healing of sore openings for a long time, bronchial asthma in children, chronic hepatitis B, chronic nephritis and viral myocarditis. For qi-tonifying, it is advisable to use it after stir-baking with honey; for stopping sweating, promoting diuresis, expelling pus and promoting granulation and muscle growth, it is advisable to use it raw.

[0027] "Codonopsis Root" is the dried root of Codonopsis pilosula (Franch.) Nannf., Codonopsis pilosula Nannf. var. modesta (Nannf.) L.T. Shen or Codonopsis tangshen Oliv. of the Campanulaceae family. It is neutral in nature and sweet in taste. It belongs to the Spleen Meridian and Lung Meridian. It can invigorate the middle-jiao and replenish qi, strengthen the spleen and nourish the lung. It belongs to the qi-tonifying herbs under the classification of tonifying deficiency herbs. For promoting the production of body fluid and nourishing blood, it is advisable to use it raw; for strengthening the spleen and nourishing the lung, it is advisable to use it after stir-baking with honey. It is used to treat weakness of the spleen and lung, shortness of breath and palpitation, poor appetite and loose stools, dyspnea with asthma, and internal heat with thirst.

[0028] "Dryopteris Rhizome" is the rhizome of Dryopteris crassirhizoma Nakai of the Dryopteridaceae family. It is slightly cold in nature and bitter in taste. It belongs to the Liver Meridian and Stomach Meridian. Dryopteris Rhizome can expel worms, stop bleeding, clear heat and detoxify. It belongs to the anthelmintic herbs. It is used to treat abdominal pain due to worm accumulation, pyogenic infections, mumps swelling, metrorrhagia and menorrhagia, and prevent and treat influenza, etc. For expelling worms and clearing heat and detoxifying, it is used raw; for stopping bleeding, it is stir-fried until charred.

[0029] "Trollius Flower" is the dried flower of Trollius chinensis Bunge. of the Ranunculaceae family. It is slightly cold in nature and bitter in taste. It belongs to the Lung Meridian and Stomach Meridian. It can clear heat and detoxify, reduce swelling and improve eyesight. It belongs to the heat-clearing and detoxifying herbs under the classification of heat-clearing herbs. It is mainly used to treat common cold with fever, sore throat, mouth ulcers, gingival swelling and pain, gingival bleeding, redness and swelling of the eyes, furuncles and carbuncles, acute tympanitis, and acute lymphangitis.

[0030] "Plantain Seed" is the seed of Plantago asiatica L. of the Plantaginaceae family. It is slightly cold in nature and sweet in taste. It belongs to the Kidney Meridian, Liver Meridian, Lung Meridian and Small Intestine Meridian. It can clear heat and promote diuresis, percolate dampness and relieve stranguria, improve eyesight and resolve phlegm. It belongs to the diuretic and stranguria-relieving herbs under the classification of herbs for promoting diuresis and percolating dampness. It is used to treat stranguria with heat syndrome, calculus stranguria, dysuria, turbid urine, edema, diarrhea caused by dampness, corneal opacity, and dim and painful eyes due to wind-heat, etc.

[0031] "Mentha" is the aerial part of the plant Mentha haplocalyx Briq. of the Lamiaceae family. It is cool in nature and pungent in taste. It belongs to the Lung Meridian and Liver Meridian. It disperses wind-heat, clears the head, and promotes eruption of measles. It belongs to the pungent-cool exterior-resolving herbs under the classification of exterior-resolving herbs. It is used to treat wind-heat cold, headache, red eyes, sore throat, itchy skin, and unsmooth eruption of measles.

[0032] "Dandelion" is the whole herb with roots of the plant Taraxacum mongolicum Hand.-Mazz. of the Asteraceae family. It is cold in nature and bitter and sweet in taste. It belongs to the Liver Meridian and Stomach Meridian. It clears heat and detoxifies, promotes diuresis and dissipates nodules. It belongs to the heat-clearing and detoxifying herbs under the classification of heat-clearing herbs. It is used to treat acute mastitis, lymphadenitis, scrofula, furuncles, acute conjunctivitis, common cold with fever, acute tonsillitis, acute bronchitis, gastritis, hepatitis, cholecystitis, and urinary tract infection.

[0033] "Licorice" is the dried root and rhizome of the plant Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat., or Glycyrrhiza glabra L. of the Fabaceae family. Licorice is neutral in nature and sweet in taste. It belongs to the Heart Meridian, Stomach Meridian, Spleen Meridian, and Lung Meridian. Licorice invigorates the spleen and replenishes qi, relieves cough and dispels phlegm, alleviates spasm and pain, and harmonizes the properties of drugs. It belongs to the qi-tonifying herbs under the classification of tonifying deficiency herbs. It is used to treat weakness of the spleen and stomach, deficiency of middle qi, cough and asthma, carbuncles and sores, abdominal pain due to spasm, relieve the strong property of drugs, and detoxify drug poisoning. It should be used raw for clearing heat and roasted for replenishing the middle.

[0034] The present invention will be further described below with reference to specific embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the scope of the present invention.

[0035] For those without specific techniques or conditions indicated in the embodiments, they shall be carried out according to the techniques or conditions described in the literature in this field or according to the product specifications. For those reagents or instruments without the manufacturer indicated, they are all conventional products that can be obtained through regular channels.

[0036] The experimental methods in the following embodiments are all conventional methods unless otherwise specified. The test materials used in the following embodiments are all commercially available products unless otherwise specified.

[0037] Preparation Example:

[0038] Example 1:

[0039] A traditional Chinese medicine composition for preventing or treating infectious mononucleosis, which is made from the following raw medicinal materials: 30 g of Astragalus membranaceus, 30 g of Codonopsis pilosula, 20 g of Dryopteris crassirhizoma, 20 g of Trollius chinensis, 20 g of Plantago asiatica, 15 g of Mentha, 15 g of Dandelion, and 10 g of Licorice.

[0040] The preparation method of the traditional Chinese medicine composition comprises the following steps: Astragalus membranaceus, Codonopsis pilosula, Dryopteris crassirhizoma, Trollius chinensis, Plantago asiatica, Mentha haplocalyx, Taraxacum mongolicum, and Glycyrrhiza uralensis are pulverized, and water is added for decoction. Decoction is carried out twice. The material-liquid ratio for the first decoction and the second decoction is both 1:4, the decoction time for the first decoction and the second decoction is both 1 hour, and the water used for the first decoction and the second decoction is distilled water. After decoction, the filtrates are mixed, concentrated under reduced pressure to obtain a concentrated solution, and then filtered through a 33 mm × 0.22 μm filter head water-based syringe filter (STEEMA) to obtain 80 mL of sterile traditional Chinese medicine stock solution.

[0041] Example 2:

[0042] A traditional Chinese medicine composition for preventing or treating infectious mononucleosis, which is prepared from the following raw medicinal materials: 25 g of Astragalus membranaceus, 25 g of Codonopsis pilosula, 25 g of Dryopteris crassirhizoma, 25 g of Trollius chinensis, 20 g of Plantago asiatica, 15 g of Mentha haplocalyx, 15 g of Taraxacum mongolicum, and 10 g of Glycyrrhiza uralensis.

[0043] The preparation method of the traditional Chinese medicine composition is the same as that in Example 1.

[0044] Control Example 1:

[0045] A traditional Chinese medicine composition, which is prepared from the following raw medicinal materials: 30 g of Astragalus membranaceus, 30 g of Codonopsis pilosula, 30 g of Trollius chinensis, 20 g of Plantago asiatica, 15 g of Mentha haplocalyx, 25 g of Taraxacum mongolicum, and 10 g of Glycyrrhiza uralensis.

[0046] The preparation method of the traditional Chinese medicine composition is the same as that in Example 1.

[0047] Control Example 2:

[0048] A traditional Chinese medicine composition, which is prepared from the following raw medicinal materials: 30 g of Astragalus membranaceus, 30 g of Codonopsis pilosula, 20 g of Dryopteris crassirhizoma, 20 g of Lonicera japonica, 20 g of Plantago asiatica, 15 g of Mentha haplocalyx, 15 g of Taraxacum mongolicum, and 10 g of Glycyrrhiza uralensis.

[0049] The preparation method of the traditional Chinese medicine composition is the same as that in Example 1.

[0050] Control Example 3:

[0051] A traditional Chinese medicine composition, which is prepared from the following raw medicinal materials: 30 g of Astragalus membranaceus, 30 g of Panax ginseng, 20 g of Dryopteris crassirhizoma, 20 g of Trollius chinensis, 20 g of Plantago asiatica, 15 g of Mentha haplocalyx, 15 g of Taraxacum mongolicum, and 10 g of Glycyrrhiza uralensis.

[0052] The preparation method of the traditional Chinese medicine composition is the same as that in Example 1.

[0053] Effect Example:

[0054] Effect Example 1: Inhibitory effect of the traditional Chinese medicine composition of the present invention on Epstein-Barr virus

[0055] 1. Experimental materials:

[0056] B95-8 cells were purchased from Wuhan Shang'en Biotechnology Co., Ltd. and cultured in RPMI-1640 medium containing 10% fetal bovine serum. The B95-8 cell line is derived from the white blood cells of a rhesus monkey exposed to Epstein-Barr virus extracted from human white blood cells. B95-8 is a continuous strain and releases a high titer of transfected Epstein-Barr virus.

[0057] 2. Experimental method:

[0058] After centrifuging the B95-8 cells in the logarithmic growth phase, they were cultured in a 96-well plate. The culture medium contained the traditional Chinese medicine compositions (10 mg / mL) prepared in Examples 1-2 and Comparative Examples 1-3, as well as the positive drug acyclovir (2 mg / L). The cells were continuously cultured at 37 °C for 48 hours, centrifuged to discard the supernatant, fresh drug-free medium was added, and then 10 μL of MTT solution (5 mg / mL) was added. After incubating at 37 °C for 4 hours, 100 μL of triple solution (10 g of SDS, 5 mL of isobutanol, 0.1 mL of 10 M HCl, dissolved in 100 mL of distilled water) was added to dissolve the crystals.

[0059] The cells treated with the solvent were used as the control group, and the absorbance (A) was measured with an enzyme-linked immunosorbent assay (ELISA) reader at a wavelength of 570 nm.

[0060] Cell proliferation inhibition rate (%) = (A value of the control group - A value of the drug group) / A value of the control group × 100%.

[0061] 3. Experimental results:

[0062] The results of the inhibitory effect of the traditional Chinese medicine compositions in each group on the proliferation of B95-8 cells are shown in Table 1. The experimental results show that the traditional Chinese medicine compositions of the present invention in the Example 1 group and the Example 2 group have a significant inhibitory effect on the proliferation of B95-8 cells, and the results are close to those of the positive drug acyclovir.

[0063] From the experimental results of each comparative example group, it can be seen that when the medicinal flavors in the traditional Chinese medicine composition of the present invention are replaced (replaced with medicinal flavors with similar effects) or removed, the inhibitory effect of the traditional Chinese medicine composition on the proliferation of B95-8 cells is significantly reduced. This indicates that the inhibitory effect of the traditional Chinese medicine composition of the present invention on the proliferation of B95-8 cells (i.e., the inhibitory effect on Epstein-Barr virus) is closely related to the types of medicinal flavors in the traditional Chinese medicine composition.

[0064] Table 1: Results of the inhibitory effect of traditional Chinese medicine compositions in each group on the proliferation of B95-8 cells

[0065]

[0066]

[0067] Effect Example 2: Preliminary Clinical Study on the Treatment of Infectious Mononucleosis with the Traditional Chinese Medicine Composition of the Present Invention

[0068] 1. Basic Information:

[0069] Thirty children diagnosed with infectious mononucleosis (damp-heat syndrome) in the outpatient department were selected as the research objects, and were divided into a treatment group and a control group according to the order of medical treatment and the random number table method.

[0070] There were 15 cases in each group. Among them, there were 17 males and 13 females, the oldest was 11 years old, the youngest was 6 years old, the disease course was 1 - 7 days, and the average disease course was 3.48 ± 1.81 days.

[0071] 2. Diagnostic Criteria:

[0072] (1) Western Medicine Diagnostic Criteria:

[0073] It is necessary to simultaneously meet any 3 items in Part I below and any 1 item in Part II below.

[0074] I. Clinical Symptoms: i) Fever, ii) Pharyngitis, tonsillitis, iii) Swollen cervical lymph nodes, iv) Slight enlargement of the liver and spleen (≤ 2 cm below the costal margin), v) Eyelid edema.

[0075] II. Laboratory Examinations: i) The proportion of abnormal lymphocytes (ALY) in peripheral blood ≥ 10%, ii) Positive detection of Epstein-Barr virus antiglycoprotein (VCA-IgM, EA-IgG).

[0076] (2) Traditional Chinese Medicine Diagnostic Criteria:

[0077] Main symptoms: Fever, red and swollen tonsils, scrofula in the neck, restlessness, loss of appetite, short and yellow urine, loose stools or constipation, red tongue with white greasy or yellow thick greasy coating, slippery and rapid pulse or soggy and rapid pulse.

[0078] Secondary symptoms: Lassitude of the head and body, chest tightness and abdominal distension, stuffiness and nausea in the epigastrium, mass in the hypochondrium, sweating or no sweating.

[0079] Diagnosis: The main symptoms of fever and red and swollen tonsils must be present, 1 secondary symptom is present, combined with the tongue coating and pulse condition.

[0080] 3. Drug Treatment:

[0081] (1) Control Group:

[0082] Recombinant human interferon α1b for injection was used, 1 μg / kg (not exceeding 40 μg), once a day, intramuscular injection.

[0083] (2) Treatment Group:

[0084] On the basis of using the "control group" for treatment, additionally take the traditional Chinese medicine composition prepared in Example 1 above, 1 dose per day, and the dosage depends on the age of the child, 50 - 80 mL each time for children aged 3 - 8 years old, and 80 - 120 mL each time for children aged 8 - 12 years old.

[0085] 4. Efficacy determination:

[0086] The treatment time for the above two groups of children is 5 days.

[0087] Clinical cure: Within 2 days after administration, the body temperature drops to 37.0 °C, the enlargement of the liver, spleen, and lymph nodes disappears within one week, and life returns to normal;

[0088] Marked effect: Within 3 days after administration, the body temperature drops to 37.0 °C, and the enlargement of the liver, spleen, and lymph nodes disappears within two weeks;

[0089] Effective: Within 5 days after administration, the body temperature drops to 37.0 °C, and the enlargement of the liver, spleen, and lymph nodes disappears within three weeks;

[0090] Ineffective: After administration, there is no change or aggravation in symptoms and signs.

[0091] 5. Treatment results:

[0092] The treatment results are shown in Table 2 below. The results in Table 2 show that after adding the traditional Chinese medicine composition of the present invention (Example 1) to the use of recombinant human interferon α1b in children, the number of cured, markedly effective, and effective patients has increased significantly. The total effective rate of the treatment group can reach 100%, which is significantly higher than that of the control group (73.33%).

[0093] Table 2: The effect of the traditional Chinese medicine composition of the present invention in the treatment of infectious mononucleosis

[0094]

[0095] Thus, it can be seen that the traditional Chinese medicine composition of the present invention has a significant treatment effect, small side effects, quick onset, short course of treatment, and high cure rate in the treatment of children with infectious mononucleosis.

[0096] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. In this way, provided that these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalent technologies, the present invention is also intended to include these modifications and variations.

Claims

1. A Chinese medicine composition for preventing or treating infectious mononucleosis, characterized in that: The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10-50 parts of astragalus, 10-50 parts of codonopsis, 5-40 parts of cyperus rotundus, 5-40 parts of trollius, 5-40 parts of plantain seeds, 1-30 parts of mint, 1-30 parts of dandelion and 1-20 parts of liquorice.

2. The Chinese medicine composition according to claim 1, characterized in that: The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10-40 parts of astragalus, 10-40 parts of codonopsis, 10-30 parts of cyperus rotundus, 10-30 parts of trollius, 10-30 parts of plantain seeds, 1-20 parts of mint, 1-20 parts of dandelion and 1-15 parts of liquorice.

3. The Chinese medicine composition according to claim 2, characterized in that: The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 30 parts of astragalus, 30 parts of codonopsis, 20 parts of cyperus rotundus, 20 parts of trollius, 20 parts of plantain seeds, 15 parts of mint, 15 parts of dandelion and 10 parts of liquorice.

4. The Chinese medicine composition according to claim 1, characterized in that: The traditional Chinese medicine composition has the effects of invigorating qi and raising yang, nourishing blood and promoting body fluid, and clearing away heat and detoxifying.

5. The method for preparing the Chinese medicine composition according to any one of claims 1 to 4, characterized in that: The preparation method comprises the following steps: crushing astragalus, codonopsis, cyperus rotundus, trollius, plantain, mint, dandelion and liquorice, adding water to boil, mixing filtrate and concentrating to obtain concentrated solution.

6. The preparation method according to claim 5, characterized in that: The decoction is performed twice. After the first decoction for 1-3 hours, a primary filtrate is obtained by filtering. The filter residue is then decocted with water for 1-3 hours, and a secondary filtrate is obtained by filtering. The primary filtrate and the secondary filtrate are combined.

7. The preparation method according to claim 6, characterized in that: The material-to-liquid ratio of the first decoction and the second decoction was 1:4, the decoction time of the first decoction and the second decoction was 1 hour, and the water used in the first decoction and the second decoction was distilled water.

8. Use of the Chinese medicine composition according to any one of claims 1 to 4 or the Chinese medicine composition prepared by the preparation method according to any one of claims 5 to 7 in preparing a medicament for preventing or treating infectious mononucleosis.

9. Use of the Chinese medicine composition according to any one of claims 1 to 4 or the Chinese medicine composition prepared by the preparation method according to any one of claims 5 to 7 in the preparation of a drug for eliminating Epstein-Barr virus (EV virus).

10. The use according to claim 8 or claim 9, characterized in that: The dosage form of the drug is capsule, granule, tablet, liquid preparation, drop pill, spray, rectal preparation or injection.

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