Lawn grass dwarfing agent and preparation method thereof

Abstract

The invention belongs to the field of plant growth regulators, and relates to a turfgrass dwarfing agent and a preparation method thereof. Comprising the following components in percentage by weight: 95 to 99 percent of bamboo growth-control hormone extracting solution and 80 to 95 percent of water in the bamboo growth-control hormone extracting solution, wherein the bamboo growth-control hormone extracting solution is prepared from the following components in percentage by weight: 95 to 99 percent of bamboo growth-control hormone extracting solution and 80 to 95 percent of chitosan; the ratio of the chitosan is 1-5%. The method has the beneficial effects that the growth-control active ingredient extracting solution of the grown bamboo is mixed with the chitosan, so that the dwarfing of the turfgrass is controlled, and the trimming frequency of the turfgrass is reduced. The components used in the invention belong to pure natural materials, and are green and environment-friendly. Forestry bamboos are used for processing leftovers of bamboo boards and bamboo furniture, resources are fully utilized, and economic benefits of the bamboo industry are benefited. The damage to lawn grass caused by conventional long-term use of chemical control chemicals (trinexapac-ethyl and the like) is changed.

Description

Technical Field

[0001] The present invention relates to a dwarfing agent for plant growth regulators, and particularly to a reagent that inhibits the growth of turfgrass and reduces the number of mowing times. Background Art

[0002] Turf is a core component of landscape and functional spaces such as urban green spaces, sports fields, and golf courses. According to statistics, the annual average consumption of water resources for turf maintenance globally exceeds 12 billion cubic meters, and the energy consumption and carbon emissions caused by high-frequency mechanical mowing (usually 1 - 3 times a week) account for more than 40% of the total maintenance cost. Frequent mowing not only exacerbates resource waste but also weakens the photosynthetic ability of turfgrass and reduces its ecological adaptability such as resistance to pests and diseases and drought tolerance, forming a vicious cycle of "mowing dependence - ecological degradation".

[0003] Plant dwarfing agents can effectively slow down the longitudinal growth of stems and leaves by regulating endogenous hormones (such as inhibiting gibberellin synthesis) or interfering with the cell elongation mechanism, thereby reducing the mowing frequency. Currently, commonly used dwarfing agents include paclobutrazol (delaying growth by inhibiting sterol synthesis), uniconazole (strong gibberellin antagonism), chlormequat chloride (inhibiting cytokinin activity), etc., but their action targets, timeliness, and plant responses are significantly different. For example, paclobutrazol is likely to remain in the soil in gramineous plants and may inhibit the growth of subsequent crops; when the concentration of chlormequat chloride is too high, it will cause leaf deformity and restricted root development. Most of the existing dwarfing agents are chemical reagents, which are not environmentally friendly when used, and are in solid or powder form and need to be paired with water-soluble fertilizers when used. In addition, bamboo extract (bamboo element) can be applied to plant dwarfing, but its application effect is limited and the inhibitory effect needs to be further improved. Summary of the Invention

[0004] Therefore, the present invention uses a natural dwarfing agent, takes common turfgrass as the object, and explores indicators such as plant height, tillering, and chlorophyll content at multiple concentration gradients. Screen dwarfing schemes with low cost and low ecological risk to provide technical support for the sustainable management and precision maintenance of turf. Thus, an environmentally friendly dwarfing agent is provided.

[0005] To achieve the above object, the present invention provides a turfgrass dwarfing agent, which comprises bamboo controlled auxin extract and chitosan, and the weight percentage components are as follows: The proportion of bamboo controlled auxin extract is 95 - 99%; the mass proportion of water in the bamboo controlled auxin extract is 80% to 95%; The proportion of chitosan is 1 - 5%.

[0006] Preferably, the proportion of chitosan is 1.5%, and the proportion of bamboo controlled auxin extract is 98.5%. The content of the active ingredient in the bamboo controlled auxin extract is 10% ± 2%; Preferably, the turfgrass includes turfgrass for urban green spaces, turfgrass for sports fields, and turfgrass for golf courses.

[0007] Preferably, the dwarfing agent is used to increase the chlorophyll content.

[0008] Preferably, the dwarfing agent is used to inhibit the elongation of tillers.

[0009] Preferably, the dwarfing agent is used for tillering.

[0010] The present invention provides a turfgrass dwarfing agent, a preparation of the turfgrass dwarfing agent diluted with water, and the dilution multiple by volume is within 1000 times.

[0011] The preparation method of the turfgrass dwarfing agent provided by the present invention includes the following steps: Step 1: Take bamboo and crush it into 5 - 10 mm debris; Step 2: Put the bamboo debris into an autoclave and add water, with a pressure of 1.0 - 1.5 MPa, a temperature of 100°C - 150°C, and a time of 2 - 3 hours; Step 3: After the temperature of the autoclave drops below 50°C, put the bamboo particle and hot water mixture into a high - speed centrifuge, use a filter cloth with 150 - 250 meshes to separate the bamboo - controlled auxin extract; Step 4: Mix the bamboo - controlled auxin extract with chitosan to obtain the turfgrass dwarfing agent.

[0012] Preferably, the particle size of the debris is 8 mm; the pressure of the autoclave is 1.2 MPa; the temperature of the autoclave is 120°C; the filter cloth is 200 meshes.

[0013] Preferably, the bamboo used is the waste material from bamboo product processing; the waste material is bamboo processed into bamboo boards.

[0014] Preferably, the waste material is the debris and / or bamboo powder of bamboo furniture.

[0015] The beneficial effects of the present invention are as follows: 1. The present invention uses the extract of the growth - controlling active ingredients of mature bamboo, mixes it with chitosan to control the dwarfing of turfgrass, and reduces the mowing frequency of turfgrass.

[0016] 2. The components used in the present invention belong to natural materials, which are green and environmentally friendly.

[0017] 3. Take the waste materials from processing bamboo into bamboo boards and bamboo furniture of mature bamboo, make full use of resources, and are beneficial to the economic benefits of the bamboo industry.

[0018] 4. Change the harm to turfgrass caused by the conventional long - term use of chemical control agents (such as trinexapac - ethyl, etc.). Description of the Drawings

[0019] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following will briefly introduce the accompanying drawings required for the description of the specific embodiments or the prior art. Obviously, the accompanying drawings in the following description are some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings.

[0020] Figure 1 It is a statistical bar chart of the effects of different dwarfing agents and concentrations on the plant height of tall fescue; Figure 2 It is an observed physical diagram of the effects of different dwarfing agents and concentrations on the plant height of tall fescue; Figure 3 It is a statistical bar chart of the effects of different dwarfing agents and concentrations on the leaf morphology of tall fescue. From left to right, they are the statistics of leaf length, leaf width, and leaf area; Figure 4 It is a statistical bar chart of the effects of different dwarfing agents and concentrations on the chlorophyll content of tall fescue; Figure 5 It is an observed physical diagram of the effects of different dwarfing agents and concentrations on the chlorophyll content of tall fescue; Figure 6 It is an observed physical diagram of the effects of different dwarfing agents and concentrations on the stem elongation and tillering of tall fescue; Figure 7 It is an observed physical diagram of the effects of different dwarfing agents and concentrations on the stem of tall fescue after 120 days of treatment; Figure 8 It is an observed physical diagram of the effects of different dwarfing agents and concentrations on the roots of tall fescue after 120 days of treatment.

[0021] CK in the accompanying drawings represents the control group without spraying dwarfing agent; FF-700 represents the application of the turfgrass dwarfing agent of the present invention, that is, the bamboo control auxin extract and chitosan diluted 700 times with water; FF-600 represents the application of the turfgrass dwarfing agent of the present invention diluted 600 times with water; FF-500 represents the application of the turfgrass dwarfing agent of the present invention diluted 500 times with water; ZY-300 represents the application of the bamboo control auxin extract diluted 300 times with water; ZY-200 represents the application of the bamboo control auxin extract diluted 200 times with water; ZY-100 represents the application of the bamboo control auxin extract diluted 100 times with water; Zhusu is the bamboo control auxin extract. Specific embodiments

[0022] The technical solution of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of them. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative work belong to the scope of protection of the present invention. Example 1:

[0023] The present invention provides a turfgrass dwarfing agent, which comprises a bamboo control auxin extract and chitosan, and the components are in weight percentages as follows: the bamboo control auxin extract accounts for 95-99%; chitosan accounts for 1-5%. Among them, the proportion of water in the bamboo control auxin extract is 80% to 95%, and the rest are substances extracted from bamboo; As a preferred embodiment, the proportion of chitosan is 1.5%, and the proportion of the bamboo control auxin extract is 98.5%; among them, the proportion of water in the bamboo control auxin extract is 90%, and the remaining 10% are the active ingredients, that is, the components leached from bamboo. Dilution and experiments are carried out below at this concentration ratio. The turfgrass dwarfing agent of the present invention can be diluted with water for actual use, and the dilution volume multiple is within 1000 times, such as diluting 300 times, 500 times, 600 times and 700 times for the following experiments. Example 2:

[0024] The preparation method of the turfgrass dwarfing agent provided by the present invention comprises the following steps: Step 1: Take bamboo and crush it into 5-10 mm debris; Step 2: Put the bamboo debris into an autoclave and add water, with a pressure of 1.0-1.5 MPa, a temperature of 100°C-150°C, and a time of 2-3 hours; Step 3: After the temperature of the autoclave drops below 50°C, put the bamboo particle and hot water mixture into a high-speed centrifuge, and use a filter cloth with 150-250 meshes to separate the bamboo control auxin extract; the mass proportion of water in the bamboo control auxin extract is 80% to 95%; for example, an extract with a mass proportion of water of 90% is obtained; Step 4: Mix the bamboo control auxin extract with chitosan to obtain the turfgrass dwarfing agent.

[0025] Preferably, the particle size of the debris is 8 mm; the pressure of the autoclave is 1.2 MPa; the temperature of the autoclave is 120°C; the filter cloth is 200 meshes.

[0026] Preferably, the bamboo used is the waste material from bamboo product processing; the waste material is bamboo for processing bamboo boards.

[0027] Preferably, the waste material is the debris and / or bamboo powder of bamboo furniture.

[0028] Experimental Example: The test materials were turfgrass creeping bentgrass and tall fescue, which were widely applicable to golf courses, football fields and green lawns in northern China. There were 2 kinds of test dwarfing agents, namely the extract of bamboo active substances (the extract of bamboo controlling auxin, or called zhusu), and the specific dilution concentrations are shown in Table 1.

[0029]

[0030] The test was carried out at the Plant Center of Beijing Forestry University from February to April 2025. Select plump and consistent creeping bentgrass and tall fescue seeds, and directly sow them in flowerpots with the size of 20 cm × 20 cm (length × height). Use the sand of golf course as the substrate, and the seeding rates were 10 g·m -2 and 40 g·m -2 . After sowing, wait for the lawn to form a turf and then start the dwarfing agent treatment. The test used no spraying of dwarfing agent as the control (CK), and 6 dwarfing agent treatments were set, namely FF-700, FF-600, FF-500, ZY-300, ZY-200 and ZY-100. Spray the dwarfing agent once at the beginning of the test, with the spraying amount of 0.003 ml·m -2 , spray once a week, and continuously observe. Each treatment was set with 3 replicates.

[0031] During the maintenance period, the lawn was watered following the principle of "seeing dry and seeing wet". Trim in a timely manner, and the trimming height was 35 mm. Compound fertilizer (potassium sulfate type) was applied during the planting period, with nitrogen: phosphorus: potassium = 18:5:18, purchased from Jiangsu Hanfeng Slow Release Fertilizer Co., Ltd., applied once a month, and the application amount was 30 g·m -2 . During the test, weeds were manually removed and broad-spectrum fungicides were used to control lawn diseases, etc.

[0032] Test Results: 1 Effects of different dwarfing agents and concentrations on the plant height of tall fescue: As Figure 1 and Figure 2 shown: After spraying the dwarfing agent for 45 days, the plant height of tall fescue was significantly lower than that of CK (P<0.05). The plant height of tall fescue under the FF treatment was significantly lower than that under the ZY treatment (P<0.05). The plant height of tall fescue under the ZY-300 treatment was significantly higher than that under the ZY-200 and ZY-100 treatments. It shows that the combined use of chitosan and the extract (the extract of bamboo controlling auxin) can more effectively inhibit the growth of tall fescue.

[0033] 2 Effects of different dwarfing agents and concentrations on the leaf morphology of tall fescue: As Figure 3As shown in the figure: The leaf length of tall fescue treated with dwarfing agent for 45 days was significantly lower than that of CK (P<0.05). The leaf length of tall fescue under FF-500 treatment was the lowest, significantly lower than that under ZY treatment (P<0.05). The leaf width of tall fescue under ZY-200, ZY-300 and FF-700 treatments was the highest, and the lowest under FF-500 treatment. The leaf area of tall fescue under FF-600 and FF-500 treatments was significantly lower than that of other treatments (P<0.05). It shows that the combined use of chitosan and extract (bamboo controlled auxin extract) can more effectively inhibit the elongation of tall fescue leaves and at the same time promote the widening of leaves.

[0034] 3 Effects of different dwarfing agents and concentrations on chlorophyll of tall fescue: As Figure 4 and Figure 5 shown in the figure: The chlorophyll content of tall fescue treated with dwarfing agent for 45 days was significantly higher than that of CK (P<0.05). The chlorophyll content of tall fescue under FF-600 and FF-500 treatments was higher than that of CK, ZY-300 and ZY-200 treatments (P<0.05). It shows that the combined use of chitosan and extract (bamboo controlled auxin extract) can promote the chlorophyll content of tall fescue leaves.

[0035] 4 Effects of different dwarfing agents and concentrations on stem elongation and tillering of tall fescue: As Figure 6 shown in the figure: The stem elongation and tillering of tall fescue treated with dwarfing agent for 45 days were significantly lower than that of CK (P<0.05). There was little difference in the stem node length between the treatment with bamboo element and CK treatment, but the stem nodes hardly grew under the treatment of bamboo element + chitosan, significantly lower than CK and ZY treatments (P<0.05). The tillering was consistent with the stem node length, and the tillering number under the treatment of bamboo element + chitosan was greater than that of CK and ZY treatments. It shows that the combined use of chitosan and extract (bamboo controlled auxin extract) can promote the tillering of tall fescue and inhibit the stem node elongation.

[0036] 5 Effects on tall fescue after 120 days of treatment with different dwarfing agents and concentrations: As Figure 7 shown in the figure: The plant height of tall fescue treated with FF for 120 days was the same as at the beginning, with almost no growth, and no pruning treatment was required during normal maintenance; the plant height of tall fescue treated with ZY for 120 days grew slightly. If not pruned, it would affect the lawn quality. The color of tall fescue treated with FF for 120 days was dark green; the color of tall fescue treated with ZY and CK for 120 days was yellow. Fertilization was carried out within 120 days for the above treatments, but the color of FF treatment was significantly greener than that of ZY and CK treatments. Compared with tall fescue treated for 45 days, the texture of tall fescue treated with FF for 120 days was significantly higher than that of 45 days, indicating that FF treatment significantly promoted the tillering of tall fescue. It shows that the inhibitory effect of the combined use of chitosan and extract (bamboo controlled auxin extract) is more obvious.

[0037] As Figure 8 shown: The root system of tall fescue treated with FF for 120 days is significantly more than that of CK and ZY treatments. This indicates that the combined use of chitosan and the extract (bamboo-controlled auxin extract) can not only inhibit the growth of the above-ground part of tall fescue, but also promote the growth of the underground part.

[0038] Obviously, the above embodiments are merely examples given for clear illustration and are not limitations on the implementation manners. For those of ordinary skill in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to enumerate all implementation manners here. And the obvious changes or modifications derived therefrom are still within the protection scope of the present invention.

Claims

1. A turfgrass dwarfing agent, characterized in that, It includes bamboo control auxin extract and chitosan, and the weight percentage composition is as follows: The proportion of bamboo control auxin extract is 95-99%; the mass proportion of water in the bamboo control auxin extract is 80% to 95%; The proportion of chitosan is 1-5%.

2. The turfgrass dwarfing agent according to claim 1, wherein The proportion of chitosan is 1.5%, and the proportion of bamboo control auxin extract is 98.5%; Among them, the content of the active ingredient in the bamboo control auxin extract is 10%±2%.

3. The turfgrass dwarfing agent according to claim 1, characterized in that, The turfgrass includes urban green space turfgrass, sports field turfgrass and golf course turfgrass.

4. The turfgrass dwarfing agent according to claim 1, characterized in that, The dwarfing agent is used to increase the chlorophyll content.

5. The turfgrass dwarfing agent according to claim 1, wherein The dwarfing agent is used to inhibit the elongation of culms.

6. The turfgrass dwarfing agent according to claim 1, wherein The dwarfing agent is used to promote tillering.

7. A lawn grass dwarfing agent, characterized in that, The turfgrass dwarfing agent is a preparation obtained by diluting the turfgrass dwarfing agent described in claim 1 or 2 with water, and the dilution volume multiple is within 1000 times.

8. The preparation method of the turfgrass dwarfing agent according to any one of claims 1 to 7, characterized in that, It includes the following steps: Step 1: Take bamboo and crush it into 5-10 mm debris; Step 2: Put the bamboo debris into an autoclave and add water. The mass ratio of bamboo debris to water is 1:0.5 to 1:20, the pressure is 1.0-1.5 MPa, the temperature is 100°C-150°C, and the time is 2-3 hours; Step 3: After the temperature of the autoclave drops below 50°C, put the mixture of bamboo particles and hot water into a high-speed centrifuge, and use a 150-250 mesh filter cloth to separate the bamboo control auxin extract; Step 4: Mix the bamboo control auxin extract with chitosan to obtain the turfgrass dwarfing agent.

9. The preparation method of the lawn grass dwarfing agent according to claim 8, characterized in that, The particle size of the debris is 8 mm; the pressure of the autoclave is 1.2 MPa; the temperature of the autoclave is 120°C; the filter cloth is 200 mesh.

10. The preparation method of the lawn grass dwarfing agent according to claim 8, characterized in that, The bamboo used is the waste material from processing bamboo products; the waste material is bamboo processed into bamboo boards; Or, the waste material is the debris or bamboo powder of bamboo furniture.

Images