Magnetic bead detection method and device and magnetic bead detection optical system

Abstract

According to the magnetic bead detection method and device and the magnetic bead detection optical system, laser is utilized to irradiate a magnetic bead sample to be detected, and magnetic beads are excited to generate fluorescence signals; illuminating a to-be-detected magnetic bead sample based on a contour light source to generate a contour light signal of a magnetic bead; acquiring first optical image information corresponding to the fluorescence signal; acquiring second optical image information corresponding to the contour light signal; and determining the type and concentration distribution of the magnetic beads by combining the first optical image information and the second optical image information. By acquiring the fluorescence signal and the contour light signal of the to-be-detected sample for imaging and identifying the type and concentration of the protein, the to-be-detected protein samples with different concentrations and types can be detected, so that the detection efficiency of the sample is improved.

Description

Magnetic bead detection method, device and magnetic bead detection optical system Technical Field

[0001] The present disclosure relates to the field of biotechnology, and in particular to a method and device for magnetic bead detection and a magnetic bead detection optical system. Background Art

[0002] Currently, chemiluminescence is the mainstream technology for protein testing. Chemiluminescence offers the advantages of high sensitivity, automation, and flexible configuration, and has gradually replaced traditional methods such as colloidal gold and enzyme-linked immunosorbent assays (ELISA). However, because chemiluminescence can only perform quantitative detection, simultaneous detection of multiple markers and minimal samples requires classified testing. However, classified testing is inefficient and time-consuming, leading to high costs for both time and consumables. This makes it difficult to quickly respond to the demand for test results for multiple proteins and samples of varying concentrations within a short period of time.

[0003] Summary of the Invention

[0004] The present disclosure provides a method, device, and optical system for magnetic bead detection, the main purpose of which is to achieve joint detection of proteins of different types and concentrations.

[0005] According to a first aspect of the present disclosure, there is provided a magnetic bead detection optical system, which includes: a contour light source module, an excitation light source module, and an imaging module;

[0006] The contour light source module is used to emit detection light to the sample to be detected having magnetic beads and illuminate the contours of the magnetic beads to generate contour light signals;

[0007] The excitation light source module is used to emit laser light to the sample to be detected to excite the magnetic beads to generate fluorescent signals;

[0008] The imaging module includes a light guide and an image acquisition device, wherein:

[0009] The light guide is located between the sample to be detected and the image acquisition device, and is used to transmit the contour light signal and the fluorescence signal to the image acquisition device in sequence;

[0010] The image acquisition device acquires a first optical image corresponding to the contour light signal and a second optical image corresponding to the fluorescence signal.

[0011] Optionally, the light guide is a first dichroic mirror, which is located between the sample to be detected and the excitation light source module. The laser generated by the excitation light source module is transmitted to the sample to be detected by the first dichroic mirror, and the fluorescence signal is reflected by the first dichroic mirror to the image acquisition device.

[0012] Optionally, the sample to be detected is located between the first dichroic mirror and the contour light source module, and the contour light signal generated by the detection light emitted by the contour light source module illuminating the magnetic beads is reflected by the first dichroic mirror to the image acquisition device.

[0013] Optionally, the contour light source module further includes: an LED light source and a second dichroic mirror;

[0014] The LED light source is used to emit the detection light to the second dichroic mirror;

[0015] The second dichroic mirror reflects the detection light to the sample to be detected.

[0016] Optionally, the contour light module further includes a reflective ring sleeved on the periphery of the LED light source and extending toward the second dichroic mirror.

[0017] Optionally, the reflective ring is in the shape of a tapered cylinder with a diameter increasing toward the second dichroic mirror.

[0018] Optionally, the contour light module further includes an illumination filter installed on a side of the reflective ring away from the LED light source.

[0019] Optionally, the contour light module further includes a matte cover provided on a side of the second dichroic mirror facing away from the LED light source.

[0020] Optionally, the excitation light source module includes a laser, and the laser is used to emit laser light; the first dichroic mirror transmits the laser light to the sample to be detected.

[0021] Optionally, the excitation light source module further includes: a reflector;

[0022] The reflecting mirror is used to reflect the laser light emitted by the laser to the first dichroic mirror.

[0023] Optionally, the image acquisition device includes an image sensor and a fluorescence filter located between the image sensor and the light guide; the fluorescence filter filters the collected fluorescence signal transmitted by the first dichroic mirror, and transmits the filtered fluorescence signal to the image acquisition device.

[0024] Optionally, the image acquisition device further includes: a telecentric lens located between the light guide and the fluorescent filter.

[0025] Optionally, the system further comprises an imaging module adjustment and fixing module;

[0026] The imaging module adjustment and fixing module includes a first dichroic mirror adjustment component and a lens fixing and adjusting component, and is used to fix the imaging module and adjust the imaging angle.

[0027] Optionally, the system further includes an excitation light source adjustment and fixing module, including a light source fixing seat, a light source adjustment seat and a reflector adjustment assembly, which is used to fix the laser and adjust the laser light path.

[0028] According to a second aspect of the present disclosure, a magnetic bead detection device is provided, comprising the magnetic bead detection optical system described in the first aspect of the present disclosure.

[0029] Optionally, the device further includes an analysis module connected to the imaging module, the analysis module receiving the first optical image and the second optical image and performing the following steps:

[0030] identifying different magnetic bead profiles in the sample to be detected according to the first optical image, and classifying the magnetic beads in the sample to be detected according to the different magnetic bead profiles;

[0031] Identifying fluorescence signals of magnetic beads of different concentrations in the sample to be detected according to the second optical image, and quantitatively detecting the different concentrations of the magnetic beads according to the fluorescence signals;

[0032] The concentration distribution of different types of magnetic beads is statistically analyzed by combining the magnetic bead profile and the fluorescent grayscale fluorescence signal.

[0033] According to a third aspect of the present disclosure, a method for magnetic bead detection is provided, comprising:

[0034] Use laser to irradiate the magnetic bead sample to be detected, and excite the magnetic beads to produce fluorescent signals;

[0035] Illuminating the magnetic bead sample to be detected based on a contour light source to generate a contour light signal of the magnetic beads;

[0036] Acquiring first optical image information corresponding to the fluorescent signal;

[0037] Acquiring second optical image information corresponding to the contour light signal;

[0038] The type and concentration distribution of the magnetic beads are determined based on the first optical image information and the second optical image information.

[0039] Optionally, before irradiating the magnetic bead sample to be detected with laser light, the method further includes:

[0040] The magnetic bead sample to be detected is dyed based on a preselected dye; wherein the wavelength of the laser does not include the fluorescence emission spectrum of the preselected dye.

[0041] Optionally, after irradiating the magnetic bead sample to be detected with laser light, the method further includes:

[0042] A light-removing mask is used to eliminate background light from the laser light passing through the magnetic bead sample to be detected.

[0043] Optionally, illuminating the magnetic bead sample to be detected based on a contour light source includes:

[0044] Straightening the detection light generated by the contour light source;

[0045] The uniformly straightened illumination light is reflected by a dichroic mirror to illuminate the magnetic bead sample to be detected and the contour information of the magnetic beads; wherein the wavelength band of the illumination light includes the fluorescence excitation spectrum of the magnetic bead sample to be detected.

[0046] Optionally, acquiring first optical image information corresponding to the fluorescence signal includes:

[0047] Reflecting the fluorescence signal into an image acquisition device based on a dichroic mirror;

[0048] The first optical image corresponding to the magnetic bead sample to be detected is obtained according to the image acquisition device.

[0049] Optionally, the acquiring second optical image information corresponding to the contour light signal includes:

[0050] Reflecting the contour light signal into an image acquisition device based on a dichroic mirror;

[0051] The second optical image corresponding to the magnetic bead sample to be detected is obtained according to the image acquisition device.

[0052] According to a fourth aspect of the present disclosure, there is provided an electronic device, including:

[0053] at least one processor; and

[0054] a memory communicatively connected to the at least one processor; wherein,

[0055] The memory stores instructions that can be executed by the at least one processor, and the instructions are executed by the at least one processor to enable the at least one processor to perform the method described in the third aspect.

[0056] According to a fifth aspect of the present disclosure, a non-transitory computer-readable storage medium storing computer instructions is provided, wherein the computer instructions are used to enable the computer to execute the method described in the third aspect.

[0057] According to a sixth aspect of the present disclosure, a computer program product is provided, comprising a computer program, wherein the computer program implements the method as described in the third aspect above when executed by a processor.

[0058] The present disclosure provides a method, apparatus, and optical system for magnetic bead detection. The method utilizes laser light to illuminate a sample of magnetic beads to be detected, stimulating the beads to produce a fluorescent signal. A contour light source illuminates the sample to be detected, generating a contour light signal of the beads. First optical image information corresponding to the fluorescent signal is acquired. Second optical image information corresponding to the contour light signal is acquired. Combining the first and second optical image information, the type and concentration distribution of the magnetic beads are determined. Compared to related technologies, the present embodiment utilizes imaging to identify the type and concentration of proteins by acquiring the fluorescent signal and contour light signal of the sample to be detected. This allows for the detection of protein samples of varying concentrations and types, thereby improving sample detection efficiency.

[0059] It should be understood that the content described in this section is not intended to identify the key or important features of the embodiments of the present application, nor is it intended to limit the scope of the present application. Other features of the present application will become easily understood through the following description. BRIEF DESCRIPTION OF THE DRAWINGS

[0060] The accompanying drawings are used to better understand the present invention and do not constitute a limitation of the present invention.

[0061] FIG1 is a schematic diagram of an optical system for detecting magnetic beads according to an embodiment of the present disclosure;

[0062] FIG2 is a schematic diagram of an optical path of a magnetic bead detection optical system provided by an embodiment of the present disclosure;

[0063] FIG3 is a cross-sectional view along line AA when the magnetic bead detection optical system shown in FIG1 is used for detection according to an embodiment of the present disclosure;

[0064] FIG4 is a schematic structural diagram of a contour light source module in the magnetic bead detection optical system shown in FIG3 ;

[0065] FIG5 is a schematic structural diagram of an image acquisition device provided by an embodiment of the present disclosure;

[0066] FIG6 is a schematic diagram of another perspective of a magnetic bead detection optical system provided by an embodiment of the present disclosure;

[0067] FIG7 is an enlarged schematic diagram of a portion P of the magnetic bead detection optical system shown in FIG6 ;

[0068] FIG8 is a schematic structural diagram of a reflector adjustment assembly provided by an embodiment of the present disclosure;

[0069] FIG9 is an exploded schematic diagram of the reflector adjustment assembly shown in FIG8 ;

[0070] FIG10 is a schematic structural diagram of a magnetic bead detection device provided in an embodiment of the present disclosure;

[0071] FIG11 is a schematic flow chart of a magnetic bead detection method provided in an embodiment of the present disclosure;

[0072] FIG12 is a schematic block diagram of an example electronic device 400 provided in accordance with an embodiment of the present disclosure. DETAILED DESCRIPTION

[0073] The following description of exemplary embodiments of the present disclosure is made in conjunction with the accompanying drawings, including various details of the embodiments of the present disclosure to facilitate understanding. These details should be considered as merely exemplary. Therefore, those skilled in the art will recognize that various changes and modifications may be made to the embodiments described herein without departing from the scope and spirit of the present disclosure. Similarly, for the sake of clarity and conciseness, descriptions of well-known functions and structures are omitted in the following description.

[0074] The following describes the magnetic bead detection method, device and magnetic bead detection optical system according to the embodiments of the present disclosure with reference to the accompanying drawings.

[0075] FIG1 is a schematic diagram of a magnetic bead detection optical system provided by an embodiment of the present disclosure. As shown in FIG1 , the protein joint detection device includes: a contour light source module 11, an excitation light source module 12, and an imaging module 13. It should be understood that the "magnetic beads" described in this application can be coded microspheres, which can be superparamagnetic microspheres with a small particle size, and / or microspheres with metal particles (which can generate magnetism under the action of an external magnetic field). The shape of the magnetic beads is not limited and can be irregular. The type of magnetic beads can be identified from the spherical shape based on the contour information in the image obtained under the contour light.

[0076] The contour light source module 11 is used to emit detection light to the sample to be detected having magnetic beads and illuminate the contours of the magnetic beads to generate contour light signals.

[0077] Specifically, in the embodiment of the present disclosure, as shown in FIG1 , the contour light source module 11 is disposed above the sample to be detected. It should be noted that the contour light source module 11 is used to provide a light source for the sample to be detected, and it is sufficient that the light emitted by the contour light source module 11 can illuminate the sample to be detected. The embodiment of the present disclosure does not limit the installation position of the contour light source module 11. For example, the contour light source module 11 can also be installed below the sample to be detected. The foregoing description is merely exemplary and does not constitute a limitation of the present disclosure.

[0078] The excitation light source module 12 is used to emit laser light to the sample to be detected, so as to excite the magnetic bead sample to generate a fluorescent signal.

[0079] Please continue to refer to Figure 1. The excitation light source module 12 is arranged below the magnetic bead sample to be detected. The excitation light source module 12 is used to generate laser and irradiate the sample to be detected to generate fluorescence; the wavelength of the excitation light source of the excitation light source module 12 matches the fluorescence excitation spectrum of the magnetic bead sample to be detected carrying the dye, and is away from the fluorescence emission spectrum of the dye. The dye is pre-selected according to the wavelength of the laser generated by the excitation light source module 12.

[0080] As shown in Figures 1-3, the imaging module 13 includes a light guide 131 and an image acquisition device 132, wherein the light guide 131 is located between the sample to be detected and the image acquisition device 132, and is used to transmit and guide the contour light signal and the fluorescence signal to the image acquisition device 132 in sequence.

[0081] The image acquisition device 132 acquires a first optical image corresponding to the contour light signal and a second optical image corresponding to the fluorescence signal.

[0082] In order for the contour light source module 11 to illuminate the contours of the magnetic bead sample to be detected, the wavelength range of the light source generated by the contour light source module 11 must include the wavelength range of the fluorescence emitted by the magnetic bead sample to be detected. The light guide 131 can transmit the excitation light source generated by the excitation light source module 12 to the magnetic bead sample to be detected, thereby stimulating the fluorescence. The light guide 131 reflects the magnetic bead contours and the excited fluorescence generated by the contour light source module 11 to the image acquisition device 132.

[0083] The present disclosure provides a magnetic bead detection optical system that utilizes laser light to illuminate a sample of magnetic beads to be detected, stimulating the beads to produce a fluorescent signal. A contour light source illuminates the sample to be detected, generating a contour light signal of the beads. First optical image information corresponding to the fluorescent signal is acquired. Second optical image information corresponding to the contour light signal is acquired. Combining the first and second optical image information, the system determines the type and concentration distribution of the magnetic beads. Compared to related technologies, the present disclosure utilizes imaging to obtain the fluorescent signal and contour light signal of the sample to be detected, enabling identification of the type and concentration of proteins associated with the corresponding magnetic beads. This system can detect protein samples of varying concentrations and types, thereby improving sample detection efficiency.

[0084] Furthermore, in a possible implementation of this embodiment, as shown in Figure 2, the light guide 131 is a first dichroic mirror 1311, and the first dichroic mirror 1311 is located between the sample to be detected 20 and the excitation light source module 12. The laser generated by the excitation light source module 12 is transmitted to the sample to be detected 20 by the first dichroic mirror 1311, and the fluorescence signal is reflected by the first dichroic mirror 1311 to the image acquisition device 132.

[0085] Specifically, in the disclosed embodiment, as shown in FIG3 , FIG3 is a cross-sectional view taken along line AA of the magnetic bead detection optical system shown in FIG1 , when detecting the sample 20 to be detected. A first dichroic mirror 1311 transmits the laser light generated by the excitation light source module 12 to the sample 20 to be detected, and then reflects the generated fluorescence signal to the image acquisition device 132 . The position of the first dichroic mirror 1311 is adjustable to achieve an adjustable optical path; thereby, the laser light generated by the excitation light source module 12 can better illuminate the magnetic beads in the sample 20 to be detected, thereby generating a fluorescence signal, which is then reflected to the image acquisition device 132.

[0086] Furthermore, in a possible implementation of this embodiment, as shown in Figure 2, the sample to be detected 20 is located between the first dichroic mirror 1311 and the contour light source module 11, and the contour light signal generated by the detection light emitted by the contour light source module 11 illuminating the magnetic beads is reflected by the first dichroic mirror 1311 to the image acquisition device 132.

[0087] Specifically, referring to FIG3 , in the embodiment of the present disclosure, after the detection light emitted by the contour light source module 11 illuminates the contours of the magnetic beads in the sample 20 to be detected, the first dichroic mirror 1311 reflects the contour light signal to the image acquisition device 132. It should be noted that the wavelength band of the detection light generated by the contour light source module 11 cannot pass through the first dichroic mirror 1311, while the laser light generated by the excitation light source module 12 can pass through the first dichroic mirror 1311.

[0088] Furthermore, in a possible implementation of this embodiment, as shown in Figure 2, the contour light source module 11 also includes: an LED light source 111 and a second dichroic mirror 112; the LED light source 111 is used to emit the detection light to the second dichroic mirror 112; the second dichroic mirror 112 reflects the detection light to the sample 20 to be detected.

[0089] Specifically, referring to FIG4 , which is a schematic diagram of the structure of a contour light source module provided in the present embodiment, the contour light source module 11 generates detection light from an LED light source 111 , which is then reflected by a second dichroic mirror 112 toward a sample 20 to be detected, thereby illuminating the contours of the magnetic beads in the sample 20 to generate a contour light signal.

[0090] Furthermore, in one possible implementation of this embodiment, as shown in FIG2 , the contour light source module 11 further includes a reflective ring 113 disposed around the periphery of the LED light source 111 and extending toward the second dichroic mirror 112. The reflective ring 113 is tapered, with its diameter increasing toward the second dichroic mirror 112. In other embodiments of the present application, the reflective ring 113 may be replaced with, for example, a laser lens, as long as the component replacing the reflective ring can produce a focusing and uniforming effect.

[0091] Specifically, in the disclosed embodiment, referring to FIG4 , an enlarged conical cylindrical reflective ring 113 is disposed around the periphery of the LED light source 111, extending toward the second dichroic mirror 112. The reflective ring 113 straightens the detection light generated by the LED light source 111, thereby improving light utilization and illumination uniformity.

[0092] Furthermore, in a possible implementation of this embodiment, as shown in FIG2 , the contour light source module 11 further includes an illumination filter 114 installed on a side of the reflective ring 113 away from the LED light source.

[0093] Specifically, in the embodiment of the present disclosure, the detection light is filtered out by the illumination filter 114 to remove light that interferes with the detection. It should be noted that the embodiment of the present disclosure does not limit the type of illumination filter 114 used.

[0094] Furthermore, in a possible implementation of this embodiment, as shown in FIG. 2 , the contour light source module further includes a matte cover 115 provided on a side of the second dichroic mirror 112 facing away from the LED light source 111 .

[0095] Specifically, referring to Figures 3 and 4 , in the disclosed embodiment, the second dichroic mirror 112 reflects the detection light generated by the LED light source 111 toward the sample 20 to be detected. After the laser light generated by the excitation light source module 12 is transmitted to the sample 20 to be detected, some of the laser light passes through the sample 20 and exits the second dichroic mirror 112. After the second dichroic mirror 112 transmits the emitted laser light, the laser light enters the matte mask 115. The matte mask 115 has a light-absorbing surface that reflects and absorbs light multiple times, significantly reducing the amount of laser light returning to the sample 20 to be detected, lowering the imaging background. It also effectively collects the laser light and avoids stray light.

[0096] Furthermore, in one possible implementation of this embodiment, as shown in FIG2 , the excitation light source module 12 includes a laser 121 and a reflector 122. The laser 121 is configured to emit laser light, and the first dichroic mirror 1311 transmits the laser light to the sample 20 to be detected. The reflector 122 is configured to reflect the laser light emitted by the laser back to the first dichroic mirror 1311.

[0097] Specifically, in the embodiment of the present disclosure, the laser 121 irradiates the emitted laser light onto the reflector 122, which then reflects the laser light onto the first dichroic mirror 1311. The laser light then passes through the first dichroic mirror 1311 and excites the magnetic beads in the sample 20 to emit fluorescence. It should be understood that in other embodiments of the present invention, the laser 121 may be directed directly toward the sample 20 to excite the magnetic beads, without the need for a reflector.

[0098] Furthermore, in one possible implementation of this embodiment, as shown in FIG2 , the image acquisition device 132 includes an image sensor 1321 and a fluorescence filter 1322 positioned between the image sensor 1321 and the light guide 131. The fluorescence filter 1322 filters the collected fluorescence signal transmitted by the first dichroic mirror 1311 and transmits the filtered fluorescence signal to the image acquisition device 132. Preferably, the image acquisition device 132 also includes a telecentric lens 1323 positioned between the light guide 131 and the fluorescence filter. It should be understood that in other embodiments of the present invention, other industrial lenses that meet the required resolution and field of view may be used in place of the telecentric lens.

[0099] Specifically, in an embodiment of the present disclosure, as shown in FIG5 , FIG5 is a schematic diagram of the structure of an image acquisition device provided by an embodiment of the present disclosure. The fluorescence signal and the contour light signal are first transmitted to the telecentric lens 1323 via the first dichroic mirror 1311. In some achievable embodiments of the present disclosure, selecting a telecentric lens with a large field of view can increase the imaging field of view, enabling one or more photographs to be taken according to the size of a single hole of the selected detection plate. The larger the single photographing area, the fewer the number of photographs required for a single hole, the higher the detection efficiency, and the lower the detection cost. The fluorescence signal and the contour light signal are then transmitted to the fluorescence filter 1322 via the telecentric lens 1323. The fluorescence filter 1322 filters out stray light such as laser light and ambient light in the fluorescence signal and the contour light signal to reduce the impact on the imaging result. After filtering by the fluorescence filter 1322, the fluorescence signal and the contour light signal are acquired by the image sensor 1321 and converted into corresponding image information.

[0100] Furthermore, in a possible implementation of this embodiment, as shown in FIG6 , the system further includes an imaging module adjustment and fixing module;

[0101] The imaging module adjustment and fixing module 14 includes a first dichroic mirror adjustment component 141 and a lens fixing and adjusting component 142 , and is used to fix the imaging module 13 and adjust the imaging angle.

[0102] Specifically, in the disclosed embodiment, the first dichroic mirror adjustment assembly 141 primarily ensures that the first dichroic mirror is installed at a 45-degree angle of incidence and that the optical axis is aligned with the ideal optical axis as closely as possible. The lens fixing adjustment assembly 142 primarily secures the entire imaging system. Furthermore, it can adjust the position of the entire imaging module 13 in the X, Y, and Z directions, as well as the tilt and pitch angles of the imaging system, to ensure that the optical axis of the entire imaging light path coincides with the optical axis of the illumination system reflected by the first dichroic mirror 1311. This ensures that the magnetic bead hole on the detection board falls within the detection system's focusing range.

[0103] Furthermore, in a possible implementation of this embodiment, as shown in Figures 6-7, the system also includes an excitation light source adjustment and fixing module 15, including a light source fixing seat 151, a light source adjustment seat 152 and a reflector adjustment assembly 153, which is used to fix the laser 121 and adjust the laser light path.

[0104] Specifically, in the disclosed embodiment, a light source fixing base 151 is used to fix the laser 121, and a light source adjustment base 152 is primarily used to adjust the emission angle of the laser light. As shown in Figures 8-9, in a reflector adjustment assembly provided in the disclosed embodiment, a reflector adjustment assembly 153 comprises a reflector fixing frame 1531, an adjustment base 1532, and a light shielding cover 1533. The reflector adjustment assembly 153 primarily ensures that the installation angle of the reflector 122 conforms to a 45-degree incident angle and that the position of the optical axis is consistent with the ideal optical axis as much as possible.

[0105] FIG10 is a schematic structural diagram of a magnetic bead detection device provided in an embodiment of the present disclosure, wherein the device includes: a magnetic bead detection optical system 21 and an analysis module 22 .

[0106] Furthermore, in a possible implementation of this embodiment, as shown in FIG9 , the apparatus further includes an analysis module 22 connected to the imaging module. The analysis module 22 receives the first optical image and the second optical image and performs the following steps:

[0107] identifying different magnetic bead profiles in the sample to be detected 20 according to the first optical image, and classifying the magnetic beads in the sample to be detected 20 according to the different magnetic bead profiles;

[0108] Identifying fluorescence signals of magnetic beads of different concentrations in the sample 20 to be detected according to the second optical image, and quantitatively detecting the different concentrations of the magnetic beads according to the fluorescence signals;

[0109] The concentration distribution of different types of magnetic beads is statistically analyzed by combining the magnetic bead profile and the fluorescent grayscale fluorescence signal.

[0110] Corresponding to the above-mentioned magnetic bead detection device, the present invention also provides a magnetic bead detection method. Since the device embodiment of the present invention corresponds to the above-mentioned method embodiment, details not disclosed in the device embodiment can be referred to the above-mentioned method embodiment and will not be repeated in this invention.

[0111] FIG11 is a flow chart of a method for magnetic bead detection provided in an embodiment of the present disclosure.

[0112] As shown in FIG11 , the method includes the following steps:

[0113] Step 301: irradiate the magnetic bead sample to be detected with laser light to excite the magnetic beads to generate fluorescent signals.

[0114] The laser generated by the excitation light source module is used to irradiate the magnetic bead sample to generate fluorescence; the wavelength of the excitation light source of the excitation light source module matches the fluorescence excitation spectrum of the dye carried by the magnetic bead sample to be detected, while being far away from the fluorescence emission spectrum of the dye.

[0115] Step 302: Acquire first optical image information corresponding to the fluorescence signal.

[0116] Step 301 ′: illuminating the magnetic bead sample to be detected based on a contour light source to generate a contour light signal of the magnetic beads.

[0117] Using the contour light source, the type of protein coupled to the magnetic beads can be further distinguished from the contour of the magnetic beads. The contour light source module provides light to the magnetic bead sample to be tested, which can illuminate the magnetic bead sample to be tested and obtain the contour light signal of the magnetic beads.

[0118] The magnetic bead detection device obtains first optical image information about the magnetic bead sample to be detected by transmitting the obtained fluorescence to the imaging module.

[0119] Step 302 ′: Acquire second optical image information corresponding to the contour light signal.

[0120] The magnetic bead detection device obtains second optical image information about the magnetic bead sample to be detected by transmitting the obtained profile to the imaging module.

[0121] Step 303 : Determine the type and concentration distribution of the magnetic beads by combining the first optical image information and the second optical image information.

[0122] It should be understood that the order of obtaining the first optical image information and the second optical image information is not limited. For example, the sample to be detected can be irradiated with contour light to obtain the second optical image information, and then the contour light is turned on to turn on the laser light source to obtain the first optical image information.

[0123] The contours of the magnetic beads can indicate the type of protein bound to them, while the brightness of the fluorescence can indicate the concentration distribution of the beads. Based on the fluorescence and contour information reflected in the image information, the type of beads and their concentration distribution can be further determined.

[0124] The present disclosure provides a method for magnetic bead detection. The method utilizes a laser to illuminate a sample of magnetic beads to be detected, stimulating the beads to produce a fluorescent signal. A contour light source is then used to illuminate the sample to be detected, generating a contour light signal of the beads. First optical image information corresponding to the fluorescent signal is obtained. Second optical image information corresponding to the contour light signal is obtained. Combining the first and second optical image information, the type and concentration distribution of the magnetic beads are determined. Compared to related technologies, the present disclosure utilizes imaging to identify the type and concentration of proteins by acquiring the fluorescent signal and contour light signal of the sample to be detected. This method enables detection of protein samples of varying concentrations and types, thereby improving sample detection efficiency.

[0125] As a feasible approach of the present disclosure, before irradiating the magnetic bead sample to be detected with laser, the method further includes:

[0126] The magnetic bead sample to be detected is dyed based on a preselected dye; wherein the wavelength of the laser does not include the fluorescence emission spectrum of the preselected dye.

[0127] In the embodiment of the present disclosure, when performing magnetic bead detection, the magnetic bead sample to be detected needs to be dyed. The fluorescence excitation spectrum of the dye matches the wavelength of the excitation light source, and the wavelength of the excitation light source should be far away from the fluorescence emission spectrum of the dye.

[0128] As a feasible approach of the present disclosure, after irradiating the magnetic bead sample to be detected with laser, the method further includes:

[0129] A light-removing mask is used to eliminate background light from the laser light passing through the magnetic bead sample to be detected.

[0130] After passing through the magnetic bead sample, the laser light can be reflected back into its original optical path within the magnetic bead detection device, affecting the experimental results. Therefore, the laser light passing through the magnetic bead sample must be extinct. The laser light is reflected by a mirror within the extinction cover, where it is absorbed by the inner surface of the extinction cover, which is made of a light-absorbing material. This prevents stray light from interfering with the experiment and preventing the laser light from escaping the device and causing accidents.

[0131] As a feasible manner of the present disclosure, illuminating the magnetic bead sample to be detected based on a contour light source includes:

[0132] Straightening the detection light generated by the contour light source;

[0133] The uniformly straightened illumination light is reflected by a dichroic mirror to illuminate the magnetic bead sample to be detected and the contour information of the magnetic beads; wherein the wavelength band of the illumination light includes the fluorescence excitation spectrum of the magnetic bead sample to be detected.

[0134] The contour light source is straightened using a focusing device (including but not limited to a focusing lens or a reflective ring) to improve light utilization and illumination uniformity. The contour light source is filtered through an illumination filter to remove light that interferes with detection. After being reflected by a second dichroic mirror, the contour light source illuminates the contour of the magnetic bead sample to be detected. In order for the contour light source to illuminate the contour of the magnetic bead sample to be detected, the wavelength band of the contour light source must include the wavelength band of the fluorescence emitted by the magnetic bead sample to be detected.

[0135] As a feasible manner of the present disclosure, obtaining first optical image information corresponding to the fluorescence signal includes:

[0136] Reflecting the fluorescence signal into an image acquisition device based on a dichroic mirror;

[0137] The first optical information corresponding to the magnetic bead sample to be detected is obtained according to an image acquisition device.

[0138] In the disclosed embodiment, a laser is transmitted through a dichroic mirror. The laser illuminates the magnetic bead sample to be tested, generating fluorescence. The dichroic mirror then reflects the generated fluorescence to an image acquisition device. The image acquisition device then outputs the first optical information to a computer, which then further determines the type and concentration distribution of the magnetic beads.

[0139] As a feasible manner of the present disclosure, obtaining the second optical image information corresponding to the contour light signal includes:

[0140] Reflecting the contour light signal into an image acquisition device based on a dichroic mirror;

[0141] The second optical image corresponding to the magnetic bead sample to be detected is obtained according to the image acquisition device.

[0142] In the disclosed embodiment, a dichroic mirror is used to reflect detection light. The detection light illuminates the magnetic bead sample to be detected, generating a profile light signal. The dichroic mirror then reflects the profile light signal generated by the profile light source illuminating the magnetic bead sample to an image acquisition device. The image acquisition device then outputs the second optical information to a computer, which further determines the type and concentration distribution of the magnetic beads.

[0143] It should be noted that the above explanation of the method embodiment is also applicable to the device of this embodiment, and the principles are the same, which is not limited in this embodiment.

[0144] According to an embodiment of the present disclosure, the present disclosure also provides an electronic device, a readable storage medium, and a computer program product.

[0145] FIG12 shows a schematic block diagram of an example electronic device 400 that can be used to implement an embodiment of the present disclosure. The electronic device is intended to represent various forms of digital computers, such as laptop computers, desktop computers, workstations, personal digital assistants, servers, blade servers, mainframe computers, and other suitable computers. The electronic device can also represent various forms of mobile devices, such as personal digital assistants, cellular phones, smart phones, wearable devices, and other similar computing devices. The components shown herein, their connections and relationships, and their functions are merely examples and are not intended to limit the implementation of the present disclosure described and / or required herein.

[0146] As shown in Figure 12, the device 400 includes a computing unit 401, which can perform various appropriate actions and processes according to a computer program stored in a ROM (Read-Only Memory) 402 or a computer program loaded from a storage unit 408 into a RAM (Random Access Memory) 403. Various programs and data required for the operation of the device 400 can also be stored in the RAM 403. The computing unit 401, ROM 402, and RAM 403 are connected to each other via a bus 404. An I / O (Input / Output) interface 405 is also connected to the bus 404.

[0147] Various components in device 400 are connected to I / O interface 405, including an input unit 406, such as a keyboard, mouse, etc.; an output unit 407, such as various types of displays, speakers, etc.; a storage unit 408, such as a magnetic disk, optical disk, etc.; and a communication unit 409, such as a network card, modem, wireless communication transceiver, etc. Communication unit 409 allows device 400 to exchange information / data with other devices via a computer network such as the Internet and / or various telecommunication networks.

[0148] The computing unit 401 can be a variety of general and / or special processing components with processing and computing capabilities. Some examples of the computing unit 401 include, but are not limited to, a CPU (Central Processing Unit), a GPU (Graphic Processing Units), various dedicated AI (Artificial Intelligence) computing chips, various computing units that run machine learning model algorithms, a DSP (Digital Signal Processor), and any appropriate processor, controller, microcontroller, etc. The computing unit 401 performs the various methods and processes described above, such as the method of magnetic bead detection. For example, in some embodiments, the method of magnetic bead detection can be implemented as a computer software program, which is tangibly contained in a machine-readable medium, such as a storage unit 408. In some embodiments, part or all of the computer program can be loaded and / or installed on the device 400 via the ROM 402 and / or the communication unit 409. When the computer program is loaded into the RAM 403 and executed by the computing unit 401, one or more steps of the method described above can be performed. Alternatively, in other embodiments, the computing unit 401 may be configured to execute the aforementioned magnetic bead detection method in any other appropriate manner (eg, by means of firmware).

[0149] Various embodiments of the systems and techniques described herein can be implemented in digital electronic circuit systems, integrated circuit systems, FPGAs (Field Programmable Gate Arrays), ASICs (Application-Specific Integrated Circuits), ASSPs (Application-Specific Standard Products), SOCs (System on Chips), CPLDs (Complex Programmable Logic Devices), computer hardware, firmware, software, and / or combinations thereof. These various embodiments can include being implemented in one or more computer programs that are executable and / or interpreted on a programmable system that includes at least one programmable processor, which can be a special-purpose or general-purpose programmable processor that can receive data and instructions from a storage system, at least one input device, and at least one output device, and transmit data and instructions to the storage system, the at least one input device, and the at least one output device.

[0150] The program code for implementing the method of the present disclosure can be written in any combination of one or more programming languages. These program codes can be provided to a processor or controller of a general-purpose computer, a special-purpose computer, or other programmable data processing device so that when the program code is executed by the processor or controller, the functions / operations specified in the flow chart and / or block diagram are implemented. The program code can be executed entirely on the machine, partially on the machine, as a stand-alone software package, partially on the machine and partially on a remote machine, or entirely on a remote machine or server.

[0151] In the context of the present disclosure, a machine-readable medium may be a tangible medium that may contain or store a program for use by or in conjunction with an instruction execution system, device, or apparatus. A machine-readable medium may be a machine-readable signal medium or a machine-readable storage medium. A machine-readable medium may include, but is not limited to, an electronic, magnetic, optical, electromagnetic, infrared, or semiconductor system, device, or apparatus, or any suitable combination of the foregoing. More specific examples of machine-readable storage media may include an electrical connection based on one or more wires, a portable computer disk, a hard disk, RAM, ROM, EPROM (Electrically Programmable Read-Only-Memory) or flash memory, optical fiber, CD-ROM (Compact Disc Read-Only Memory), an optical storage device, a magnetic storage device, or any suitable combination of the foregoing.

[0152] To provide interaction with a user, the systems and techniques described herein can be implemented on a computer having: a display device (e.g., a CRT (Cathode-Ray Tube) or LCD (Liquid Crystal Display) monitor) for displaying information to the user; and a keyboard and pointing device (e.g., a mouse or trackball) through which the user can provide input to the computer. Other types of devices can also be used to provide interaction with the user; for example, the feedback provided to the user can be any form of sensory feedback (e.g., visual feedback, auditory feedback, or tactile feedback); and input from the user can be received in any form (including acoustic input, voice input, or tactile input).

[0153] The systems and techniques described herein can be implemented in a computing system that includes back-end components (e.g., as a data server), or a computing system that includes middleware components (e.g., an application server), or a computing system that includes front-end components (e.g., a user computer with a graphical user interface or web browser through which a user can interact with implementations of the systems and techniques described herein), or a computing system that includes any combination of such back-end components, middleware components, or front-end components. The components of the system can be interconnected by any form or medium of digital data communication (e.g., a communication network). Examples of communication networks include: LAN (Local Area Network), WAN (Wide Area Network), the Internet, and blockchain networks.

[0154] A computer system may include a client and a server. The client and server are generally remote from each other and typically interact via a communication network. This client-server relationship is established by computer programs running on the respective computers, establishing a client-server relationship. The server may be a cloud server, also known as a cloud computing server or cloud host, a host product within a cloud computing service ecosystem that addresses the management difficulties and limited scalability of traditional physical hosts and VPS services ("Virtual Private Servers" or simply "VPS"). The server may also be a server in a distributed system or a server integrated with blockchain.

[0155] It's important to note that artificial intelligence (AI) is the study of how computers can simulate certain human thought processes and intelligent behaviors (such as learning, reasoning, thinking, and planning). This encompasses both hardware and software technologies. AI hardware technologies generally include sensors, specialized AI chips, cloud computing, distributed storage, and big data processing. AI software technologies primarily encompass computer vision, speech recognition, natural language processing, machine learning / deep learning, big data processing, and knowledge graphs.

[0156] It should be understood that the various forms of the processes shown above can be used to reorder, add, or delete steps. For example, the steps described in this disclosure can be performed in parallel, sequentially, or in a different order, as long as the desired results of the technical solutions disclosed in this disclosure can be achieved. This is not limited herein.

[0157] The above specific embodiments do not constitute a limitation on the scope of protection of this disclosure. Those skilled in the art will appreciate that various modifications, combinations, sub-combinations, and substitutions may be made based on design requirements and other factors. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this disclosure shall be included within the scope of protection of this disclosure.

Claims

1. A magnetic bead detection optical system, characterized in that: include: Contour light source module, excitation light source module and imaging module; The contour light source module is used to emit detection light to the sample to be detected having magnetic beads and illuminate the contours of the magnetic beads to generate contour light signals; The excitation light source module is used to emit laser light to the sample to be detected to excite the magnetic beads to generate fluorescent signals; The imaging module includes a light guide and an image acquisition device, wherein: The light guide is located between the sample to be detected and the image acquisition device, and is used to transmit the contour light signal and the fluorescence signal to the image acquisition device in sequence; The image acquisition device acquires a first optical image corresponding to the contour light signal and a second optical image corresponding to the fluorescence signal.

2. The magnetic bead detection optical system according to claim 1, wherein: The light guide is a first dichroic mirror, which is located between the sample to be detected and the excitation light source module. The laser generated by the excitation light source module is transmitted to the sample to be detected by the first dichroic mirror, and the fluorescence signal is reflected by the first dichroic mirror to the image acquisition device.

3. The magnetic bead detection optical system according to claim 2, wherein: The sample to be detected is located between the first dichroic mirror and the contour light source module. The contour light signal generated by the detection light emitted by the contour light source module illuminating the magnetic beads is reflected by the first dichroic mirror to the image acquisition device.

4. The magnetic bead detection optical system according to claim 3, wherein: The contour light source module further includes: an LED light source and a second dichroic mirror; The LED light source is used to emit the detection light to the second dichroic mirror; The second dichroic mirror reflects the detection light to the sample to be detected.

5. The magnetic bead detection optical system according to claim 4, characterized in that: The contour light module further includes a reflective ring which is sleeved on the periphery of the LED light source and extends toward the second dichroic mirror.

6. The magnetic bead detection optical system according to claim 5, characterized in that: The reflective ring is in the shape of a tapered cylinder with a diameter increasing toward the second dichroic mirror.

7. The magnetic bead detection optical system according to claim 5, characterized in that: The contour light module further includes an illumination filter installed on a side of the reflective ring away from the LED light source.

8. The magnetic bead detection optical system according to claim 4, wherein: The contour light module further includes a matte cover provided on a side of the second dichroic mirror facing away from the LED light source.

9. The magnetic bead detection optical system according to claim 2, wherein: The excitation light source module includes a laser, and the laser is used to emit laser light; the first dichroic mirror transmits the laser light to the sample to be detected.

10. The magnetic bead detection optical system according to claim 9, wherein: The excitation light source module further includes: a reflector; The reflecting mirror is used to reflect the laser light emitted by the laser to the first dichroic mirror.

11. The magnetic bead detection optical system according to claim 2, wherein: The image acquisition device includes an image sensor and a fluorescence filter located between the image sensor and the light guide; the fluorescence filter filters the collected fluorescence signal transmitted by the first dichroic mirror and transmits the filtered fluorescence signal to the image acquisition device.

12. The magnetic bead detection optical system according to claim 11, wherein: The image acquisition device further includes a telecentric lens located between the light guide and the fluorescent filter.

13. The magnetic bead detection optical system according to claim 12, wherein: The system further comprises an imaging module adjustment and fixing module; The imaging module adjustment and fixing module includes a first dichroic mirror adjustment component and a lens fixing and adjusting component, and is used to fix the imaging module and adjust the imaging angle.

14. The magnetic bead detection optical system according to claim 10, wherein: The system also includes an excitation light source adjustment and fixing module, which includes a light source fixing seat, a light source adjustment seat and a reflector adjustment component, and is used to fix the laser and adjust the laser light path.

15. A magnetic bead detection device, characterized in that: Comprising the magnetic bead detection optical system according to any one of claims 1 to 14.

16. The magnetic bead detection device according to claim 15, characterized in that: The apparatus further includes an analysis module connected to the imaging module, the analysis module receiving the first optical image and the second optical image and performing the following steps: identifying different magnetic bead profiles in the sample to be detected according to the first optical image, and classifying the magnetic beads in the sample to be detected according to the different magnetic bead profiles; Identifying fluorescence signals of magnetic beads of different concentrations in the sample to be detected according to the second optical image, and quantitatively detecting the different concentrations of the magnetic beads according to the fluorescence signals; The concentration distribution of different types of magnetic beads is statistically analyzed by combining the magnetic bead profile and the fluorescent grayscale fluorescence signal.

17. A method for magnetic bead detection, characterized in that: include: Use laser to irradiate the magnetic bead sample to be detected, and excite the magnetic beads to produce fluorescent signals; Illuminating the magnetic bead sample to be detected based on a contour light source to generate a contour light signal of the magnetic beads; Acquiring first optical image information corresponding to the fluorescent signal; Acquiring second optical image information corresponding to the contour light signal; The type and concentration distribution of the magnetic beads are determined based on the first optical image information and the second optical image information.

18. The method according to claim 17, characterized in that Before irradiating the magnetic bead sample to be detected with laser, the method further includes: The magnetic bead sample to be detected is dyed based on a preselected dye; wherein the wavelength of the laser does not include the fluorescence emission spectrum of the preselected dye.

19. The method according to claim 17, wherein After irradiating the magnetic bead sample to be detected with laser, the method further includes: A light-removing mask is used to eliminate background light from the laser light passing through the magnetic bead sample to be detected.

20. The method according to claim 17, wherein The step of illuminating the magnetic bead sample to be detected based on a contour light source comprises: Straightening the detection light generated by the contour light source; The uniformly straightened illumination light is reflected by a dichroic mirror to illuminate the magnetic bead sample to be detected and the contour information of the magnetic beads; wherein the wavelength band of the illumination light includes the fluorescence excitation spectrum of the magnetic bead sample to be detected.

21. The method according to claim 17, wherein The acquiring first optical image information corresponding to the fluorescence signal includes: Reflecting the fluorescence signal into an image acquisition device based on a dichroic mirror; The first optical image corresponding to the magnetic bead sample to be detected is obtained according to the image acquisition device.

22. The method according to claim 17, wherein The acquiring of second optical image information corresponding to the contour light signal comprises: Reflecting the contour light signal into an image acquisition device based on a dichroic mirror; The second optical image corresponding to the magnetic bead sample to be detected is obtained according to the image acquisition device.

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