Boa oil-containing essence for fading neck lines and tightening neck skin and preparation method thereof

Abstract

The invention provides python oil-containing essence for fading neck wrinkles and tightening neck skin and a preparation method thereof, and the python oil-containing essence is prepared from the following raw materials in parts by weight: 30-50 parts of python oil, 15-20 parts of silybum marianum seed oil, 15-20 parts of simmondsia chinensis seed oil, 6-10 parts of asiaticoside, 5-8 parts of ginsenoside, 4-6 parts of acetyl octapeptide-3 and 4-6 parts of acetyl hexapeptide-8. The coenzyme Q10 accounts for 3-5 parts, and the bakuchiol accounts for 3-5 parts. The preparation method of the silybum marianum seed oil comprises the following steps: selecting silybum marianum seeds, soaking the silybum marianum seeds in a deep eutectic solvent, purifying the silybum marianum seeds, extracting silybum marianum cakes again, and mixing to obtain a silybum marianum seed oil finished product. The essence containing boa oil is scientific in formula, all the components have a synergistic effect, and the essence can fade neck wrinkles and tighten neck skin, is safe and reliable and has application value.

Description

Technical Field

[0001] The present invention relates to the field of skin care products, and particularly relates to a snake oil-containing essence for fading neck wrinkles and firming neck skin and a preparation method thereof. Background Art

[0002] The aging of neck skin is a prominent external manifestation of skin aging. As people age, the metabolism slows down, resulting in the thinning of the dermis layer of neck skin, poor elasticity, uneven collapse, and the generation of wrinkles. The number of sebaceous glands and sweat glands in the neck skin of the human body is about one-third of that of facial skin, with less sebum secretion, easy to dry, age and sag, and generate wrinkles. Therefore, better care and maintenance are needed. Currently, the following problems exist in the commercially available neck wrinkle-fading skin care products: First, the commercially available neck wrinkle-fading products contain high-concentration ingredients such as alcohol and retinol, which may irritate the neck skin, causing discomfort such as tingling and redness of the neck skin, and may even accelerate aging; Second, some commercially available products use excessive exfoliating ingredients, which will damage the skin barrier and make the skin more sensitive; Moreover, there are preservatives and oil ingredients, and some products add too many preservatives and high-oil ingredients. Long-term use may cause skin problems such as clogged pores, acne and seborrheic dermatitis. These ingredients may also affect the skin's absorption of other skin care ingredients, resulting in poor neck wrinkle-fading effects. Summary of the Invention

[0003] In view of this, the present invention provides a snake oil-containing essence for fading neck wrinkles and firming neck skin and a preparation method thereof to meet the needs of consumers for fading neck wrinkles and firming neck skin.

[0004] The technical solution of the present invention is realized as follows:

[0005] A snake oil-containing essence for fading neck wrinkles and firming neck skin, comprising the following raw materials in parts by weight: 30-35 parts of snake oil, 25-30 parts of silybum marianum seed oil, 15-20 parts of jojoba seed oil, 6-10 parts of asiaticoside, 5-8 parts of ginsenoside, 4-6 parts of acetyl octapeptide-3, 4-6 parts of acetyl hexapeptide-8, 3-5 parts of coenzyme Q10, and 3-5 parts of bakuchiol.

[0006] The preparation of silybum marianum seed oil comprises the following steps:

[0007] S1: Select silybum marianum seeds, remove impurities, stir-fry the seeds, ultrasonically crush them, and soak them with a deep eutectic solvent to obtain silybum marianum seed slurry;

[0008] S2: Purify the silybum marianum seed slurry to obtain silybum marianum seed oil I, silybum marianum seed oil and cake;

[0009] S3: Redissolve and extract the cake to obtain silybum marianum seed oil II;

[0010] S4: Mix silybum marianum seed oil Ⅰ and silybum marianum seed oil Ⅱ to obtain the finished silybum marianum seed oil;

[0011] Furthermore, in step S1, for the seed frying, first fry over low heat for 5 - 10 min, then change to high heat and fry for 8 - 15 min. When frying over high heat, introduce steam at the bottom of the frying pan, and the steam introduction rate is 20 - 35 g / min;

[0012] Furthermore, in step S1, the deep eutectic solvent is composed of levulinic acid and menthol with a mass ratio of 1 - 2:1, and the addition amount of the deep eutectic solvent is 5 - 10 times that of the silybum marianum seed powder.

[0013] Furthermore, in step S1, the ultrasonic crushing frequency is 30 - 40 kHz, and the time is 20 - 40 min; the soaking temperature is 35 - 40 °C, and the soaking time is 4 - 6 h.

[0014] Furthermore, in step S3, the cake residue is extracted by supercritical dissolution. The dissolution solution is an ethanol solution with a mass fraction of 20% - 35%. The extraction pressure is 25 - 35 MPa, the temperature is 45 - 50 °C, the CO₂ flow rate is 20 - 25 L / h, and the extraction time is 40 - 45 min.

[0015] The preparation steps of a snake oil-containing essence for lightening neck wrinkles and tightening neck skin include:

[0016] Step 1: Mix asiaticoside, ginsenoside, acetyl octapeptide-3, acetyl hexapeptide-8, coenzyme Q10, and bakuchiol and add them to an ethanol solution with a mass fraction of 30% - 35%. Stir, perform high-speed shearing, high-pressure homogenization, filter to remove impurities, perform high-pressure homogenization again, and concentrate to obtain mixture Ⅰ;

[0017] Step 2: Mix snake oil, silybum marianum seed oil, and jojoba oil and stir, perform high-pressure homogenization to obtain mixture Ⅱ;

[0018] Step 3: Mix and stir mixture Ⅰ and mixture Ⅱ, filter, and perform high-pressure homogenization to obtain the essence.

[0019] Furthermore, in steps 1 - 3, the pressure of the high-pressure homogenization is 60 - 75 MPa, the pressure-holding time is 30 - 35 min, and the temperature is 15 - 25 °C.

[0020] Furthermore, in step 1, the rotation speed of the high-speed shearing is 7000 - 7500 r / min, the temperature is 25 - 30 °C, and the time is 5 - 10 min.

[0021] Furthermore, in step 3, for the filtration, a 5 - 10 kDa ultrafiltration membrane is used for filtration.

[0022] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0023] 1. For the essence of the present invention, the compound of python oil, silybum marianum seed oil and jojoba oil has the effect of moisturizing and locking water. The extraction method of silybum marianum seed oil in the present invention improves the purity and content of active substances of silybum marianum seed oil. After compounding, it can significantly increase the water content of the skin (P﹤0.05), and has the application effect of moisturizing the skin.

[0024] 2. For the essence of the present invention, adding ingredients such as asiaticoside, ginsenoside, acetyl octapeptide-3, acetyl hexapeptide-8, coenzyme Q10 and bakuchiol promotes the metabolism of skin cells, significantly increases the activity of superoxide dismutase (SOD) in the skin, significantly reduces the content of malondialdehyde (MDA) in the skin, and effectively delays skin aging, has antioxidant and cell protection effects.

[0025] 3. The inhibition rate of the essence of the present invention on elastase is 44% at a test concentration of 50%. It is proved that it has an inhibitory effect on elastase. According to the correlation between elastase and firming and anti-wrinkle effects, it shows that the essence of the present invention has firming and anti-wrinkle effects, can fade neck wrinkles and firm the neck skin. Specific Embodiments

[0026] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention. The experimental methods used in the embodiments of the present invention are all conventional methods unless otherwise specified.

[0027] The materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial channels unless otherwise specified.

[0028] Preparation Example 1

[0029] The preparation of silybum marianum seed oil includes the following steps:

[0030] Step (1): Select silybum marianum seeds to remove impurities, stir-fry the silybum marianum seeds. First, stir-fry over low heat for 5 minutes, then change to high heat and stir-fry for 8 minutes. When stir-frying over high heat, introduce steam at the bottom of the frying pan, and the introduction amount of steam is 20 g / min. After stir-frying, perform ultrasonic crushing, with a crushing frequency of 30 kHz and a time of 20 minutes. Soak with a deep eutectic solvent, which is composed of levulinic acid and menthol with a mass ratio of 1:1. The addition amount of the deep eutectic solvent is 5 times that of the silybum marianum seed powder. The soaking temperature is 35 °C and the soaking time is 4 hours. After soaking, obtain silybum marianum seed slurry;

[0031] Step (2): Purify the silybum marianum seed slurry to obtain silybum marianum seed oil I, silybum marianum seed oil and cake;

[0032] Step (III): Redissolve and extract the cake residue through supercritical dissolution extraction. The dissolution solution is an ethanol solution with a mass fraction of 20%, the extraction pressure is 25 MPa, the temperature is 45 °C, the CO2 flow rate is 20 L / h, and the extraction time is 40 min. Silybum marianum seed oil II is obtained after extraction;

[0033] Step (IV): Mix Silybum marianum seed oil I and Silybum marianum seed oil II to obtain the finished Silybum marianum seed oil.

[0034] Preparation Example 2

[0035] The preparation of Silybum marianum seed oil includes the following steps:

[0036] Step (I): Select Silybum marianum seeds to remove impurities, stir-fry the Silybum marianum seeds. First, stir-fry over low heat for 10 min, then change to high heat and stir-fry for 15 min. When stir-frying over high heat, introduce steam at the bottom of the frying pan, and the introduction amount of steam is 35 g / min. After stir-frying, perform ultrasonic crushing with a crushing frequency of 40 kHz and a time of 40 min. Soak with a deep eutectic solvent, which is composed of levulinic acid and menthol with a mass ratio of 2:1. The addition amount of the deep eutectic solvent is 10 times that of the Silybum marianum seed powder, the soaking temperature is 40 °C, and the soaking time is 6 h.

[0037] The Silybum marianum seed slurry is obtained after soaking;

[0038] Step (II): Purify the Silybum marianum seed slurry to obtain Silybum marianum seed oil I, Silybum marianum seed oil, and cake residue;

[0039] Step (III): Redissolve and extract the cake residue through supercritical dissolution extraction. The dissolution solution is an ethanol solution with a mass fraction of 35%, the extraction pressure is 35 MPa, the temperature is 50 °C, the CO2 flow rate is 25 L / h, and the extraction time is 40 min. Silybum marianum seed oil II is obtained after extraction;

[0040] Step (IV): Mix Silybum marianum seed oil I and Silybum marianum seed oil II to obtain the finished Silybum marianum seed oil.

[0041] Preparation Example 3

[0042] The preparation of Silybum marianum seed oil includes the following steps:

[0043] Step (I): Select silybum marianum seeds, remove impurities, and stir-fry the silybum marianum seeds. First, stir-fry over low heat for 8 minutes, then switch to high heat and stir-fry for 10 minutes. When stir-frying over high heat, introduce steam at the bottom of the frying pan, with the steam introduction rate being 25 g / min. After stir-frying, perform ultrasonic crushing with a crushing frequency of 35 kHz for 30 minutes. Soak with a deep eutectic solvent, which is composed of levulinic acid and menthol in a mass ratio of 1.5:1. The addition amount of the deep eutectic solvent is 6 times that of the silybum marianum seed powder. The soaking temperature is 35 - 40 °C, and the soaking time is 5 hours.

[0044] After soaking, obtain silybum marianum seed slurry;

[0045] Step (II): Purify the silybum marianum seed slurry to obtain silybum marianum seed oil I, silybum marianum seed oil, and cake residue;

[0046] Step (III): Redissolve and extract the cake residue using supercritical dissolution extraction. The dissolution solution is an ethanol solution with a mass fraction of 30%. The extraction pressure is 30 MPa, the temperature is 50 °C, the CO2 flow rate is 25 L / h, and the extraction time is 45 minutes. After extraction, obtain silybum marianum seed oil II;

[0047] Step (IV): Mix silybum marianum seed oil I and silybum marianum seed oil II to obtain the finished silybum marianum seed oil.

[0048] Example 1

[0049] The preparation steps of the essence containing python oil in Example 1 include:

[0050] Step (I): Mix asiaticoside, ginsenoside, acetyl octapeptide - 3, acetyl hexapeptide - 8, coenzyme Q10, and bakuchiol, add them to an ethanol solution with a mass fraction of 30%, stir, and perform high - speed shearing. The rotation speed of high - speed shearing is 7000 r / min, the temperature is 25 °C, and the time is 5 minutes; then perform high - pressure homogenization. The pressure of high - pressure homogenization is 60 MPa, the holding time is 30 minutes, and the temperature is 15 °C; filter to remove impurities, perform high - pressure homogenization again. The pressure of high - pressure homogenization is 60 MPa, the holding time is 30 minutes, and the temperature is 15 °C, and then concentrate to obtain mixture I;

[0051] Step (II): Mix and stir python oil, silybum marianum seed oil prepared by the method of Preparation Example 1, and jojoba oil, and perform high - pressure homogenization. The pressure of high - pressure homogenization is 60 MPa, the holding time is 30 minutes, and the temperature is 15 °C to obtain mixture II;

[0052] Step (III): Mix and stir mixture I and mixture II, filter using a 5 kDa ultrafiltration membrane, and after filtration, perform high - pressure homogenization. The pressure of high - pressure homogenization is 60 MPa, the holding time is 30 minutes, and the temperature is 15 °C. After homogenization, obtain the essence.

[0053] Example 2

[0054] The preparation steps of the essence containing python oil in Example 2 are as follows:

[0055] Step (1): Mix asiaticoside, ginsenoside, acetyl octapeptide-3, acetyl hexapeptide-8, coenzyme Q10 and bakuchiol, add them into an ethanol solution with a mass fraction of 35%, stir, and perform high-speed shearing. The rotation speed of high-speed shearing is 7500 r / min, the temperature is 30 °C, and the time is 10 min; then perform high-pressure homogenization. The pressure of high-pressure homogenization is 75 MPa, the holding time is 35 min, and the temperature is 25 °C; filter to remove impurities, perform high-pressure homogenization again. The pressure of high-pressure homogenization is 75 MPa, the holding time is 35 min, and the temperature is 25 °C, and concentrate to obtain mixture I;

[0056] Step (2): Mix and stir python oil, silymarin oil prepared by the method of Preparation Example 2 and jojoba oil, and perform high-pressure homogenization. The pressure of high-pressure homogenization is 75 MPa, the holding time is 35 min, and the temperature is 25 °C to obtain mixture II;

[0057] Step (3): Mix and stir mixture I and mixture II, filter with a 10 kDa ultrafiltration membrane, perform high-pressure homogenization after filtration. The pressure of high-pressure homogenization is 75 MPa, the holding time is 35 min, and the temperature is 25 °C. After homogenization, the essence is obtained.

[0058] Example 3

[0059] The preparation steps of the essence containing python oil in Example 3 are as follows:

[0060] Step (1): Mix asiaticoside, ginsenoside, acetyl octapeptide-3, acetyl hexapeptide-8, coenzyme Q10 and bakuchiol, add them into an ethanol solution with a mass fraction of 35%, stir, and perform high-speed shearing. The rotation speed of high-speed shearing is 7200 r / min, the temperature is 25 °C, and the time is 8 min; then perform high-pressure homogenization. The pressure of high-pressure homogenization is 70 MPa, the holding time is 35 min, and the temperature is 20 °C; filter to remove impurities, perform high-pressure homogenization again. The pressure of high-pressure homogenization is 70 MPa, the holding time is 35 min, and the temperature is 20 °C, and concentrate to obtain mixture I;

[0061] Step (2): Mix and stir python oil, silymarin oil prepared by the method of Preparation Example 3 and jojoba oil, and perform high-pressure homogenization. The pressure of high-pressure homogenization is 70 MPa, the holding time is 30 min, and the temperature is 15 °C to obtain mixture II;

[0062] Step (III): Mix and stir Mixture I and Mixture II, filter using a 10 kDa ultrafiltration membrane, and then perform high-pressure homogenization. The pressure for high-pressure homogenization is 70 MPa, the holding time is 30 min, and the temperature is 20°C. After homogenization, the essence is obtained.

[0063] Table 1 List of Chemical Properties of the Raw Materials of the Present Invention

[0064]

[0065] Comparative Example 1

[0066] The difference from Example 3 is that in the preparation step of the essence containing python oil, the mass portion of python oil is reduced by half, asiaticoside and coenzyme Q10 are not added, and other raw materials and preparation methods are the same as those in Example 3.

[0067] Comparative Example 2

[0068] The difference from Example 3 is that in the preparation step of the essence containing python oil, in Preparation Example 3, in the preparation step of silybum marianum seed oil, the step of stir-frying silybum marianum seeds is removed, and the deep eutectic solvent immersion is replaced by immersion in a 30% by mass ethanol solution, and other raw materials and preparation methods are the same as those in Example 3.

[0069] Comparative Example 3

[0070] The difference from Example 3 is that in the preparation step of the essence containing python oil, the mass portion of silybum marianum seed oil is reduced by half, acetyl octapeptide-3 and acetyl hexapeptide-8 are not added, and other raw materials and preparation methods are the same as those in Example 3.

[0071] Commercial Control

[0072] A commercially available skin care product for reducing neck wrinkles and tightening the neck skin is used as a control. The purchased brand is Lucafia Firming Anti-Wrinkle Cream, and the main ingredients are hexapeptide, boswellia serrata extract, hyaluronic acid, trehalose, xanthan gum, aloe vera leaf extract, and dendrobium nobile.

[0073] Blank Control

[0074] Purified water is used as a blank control test.

[0075] Experiment

[0076] Kunming mice, with a body weight of (20±2) g, half male and half female, SPF grade. Eighty mice were randomly divided into 10 mice in Example 1, 10 mice in Example 2, 10 mice in Example 3, 10 mice in Comparative Example 1, 10 mice in Comparative Example 2, 10 mice in Comparative Example 3, 10 mice in the commercially available control, and 10 mice in the blank control. All the hair on the nape of the mice was removed, and 1 g / kg of 125 mg / L D-galactose was subcutaneously injected daily for 45 consecutive days to establish a subacute aging model. After 45 days, the essence prepared in Examples 1-3, Comparative Examples 1-3, the commercially available control, and the blank control were applied at 2-3 mL / mouse / time, and continuously applied for 30 days. During the experiment, the characteristics and activities of the mice were recorded every day. One hour after the last application, the mice were sacrificed by cervical dislocation, and 1 cm of the skin in the middle of the back was taken 2 in size and quickly placed in a 20% formalin solution by volume to take 1 cm 2 or so of the skin for measuring skin water content; the remaining back skin was taken for preparing skin tissue homogenate to measure skin biochemical indexes.

[0077] Measurement of skin water content:

[0078] After depilating and cleaning the skin on the nape of the neck, 1 cm of skin was cut 2 and its wet weight was precisely weighed. Immediately, it was placed in an oven and dried at 80 °C for 12 h, and its dry weight was weighed. The percentage of water content in the skin was calculated: water content rate (%) = (weight before drying - weight after drying) / weight before drying × 100%.

[0079] The test results showed (Table 1) that when the essence prepared by the methods of Examples 1-3 was applied to the epidermis of the test mice, the skin water content was significantly increased (P﹤0.05), and it had the application effect of moisturizing the skin.

[0080] Measurement of skin homogenate biochemical indexes:

[0081] Take about 0.5 g of back skin tissue for hair removal, rinse it with pre-cooled normal saline, remove subcutaneous fat and other connective tissues, dry it with filter paper, weigh its mass, measure 9 times the mass of the tissue block of pre-cooled normal saline, first pour 2 / 3 of the amount into the beaker containing the tissue block, cut the tissue block into pieces and pour it into the homogenizer tube, rinse the beaker wall with the remaining 1 / 3 normal saline, and use an internal cutting tissue homogenizer to make a 100 g / L tissue homogenate (in ice water), freeze-thaw it 3 times repeatedly to completely break the cells and make all the contents free in the liquid phase. Refer to the method in the kit instruction manual to measure the SOD activity and MDA content. Malondialdehyde (MDA) is an important biomarker, mainly used to evaluate the degree of lipid peroxidation damage, thus reflecting the degree of damage to the membrane system and the stress resistance of plants. A higher MDA level is usually associated with lipid peroxidation damage to the cell membrane, which may have an adverse effect on cell function. The measured water content, superoxide dismutase (SOD) activity and malondialdehyde (MDA) content are shown in Table 1.

[0082] Table 2 Table of water content, SOD activity and MDA content in mouse skin

[0083]

[0084]

[0085] The test results show (Table 2) that applying the essence prepared by the methods of Examples 1-3 to the epidermis of the test mice significantly increased the superoxide dismutase (SOD) activity of the skin, significantly reduced the malondialdehyde (MDA) content of the skin, and the skin care effects were all higher than those of the commercial control and the blank control, indicating that the essence of the present invention can significantly improve the SOD activity in the skin of damaged mice, while reducing the MDA content, and effectively delay skin aging, have antioxidant effects and protect cells.

[0086] Elastase detection test

[0087] Elastase is a proteolytic enzyme with extremely high selectivity and specificity, which has a catalytic hydrolysis effect on many polypeptide bonds containing carboxyl groups of amino acids, and will decompose elastin in connective tissue proteins. Relevant literature shows that elastase inhibitors can effectively inhibit elastase activity, slow down the degradation rate of elastin, and play a role in delaying aging and reducing wrinkles.

[0088] In this experiment, the double antibody sandwich method was used to detect the inhibition rate of the sample on elastase. The inhibitory factor that may exist in the sample inhibits the activity of elastase. The still-active elastase binds to the biotin-labeled antibody, and then binds to streptavidin-HRP to form an immune complex. After incubation and washing, a chromogenic solution is added for color development. After terminating the reaction, the absorbance value is measured by an enzyme-linked immunosorbent assay (ELISA) reader. By comparing and analyzing the test data of Examples 1-3 in the experimental group and the blank control, the inhibitory effect of the sample on elastase is evaluated. From this data, the firming and anti-wrinkle efficacy of the cosmetic can be relatively reasonably evaluated.

[0089] Detection method: WT-HZ-504-3006 "Test Work Instruction for the Firming and Anti-Wrinkle Efficacy of Cosmetics (Elastase Inhibition Method)"; Detection report number: WTA24F04090012M; Test date: April 24, 2024.

[0090] Table 3 Analysis of the Inhibition Rate of Elastase Activity

[0091]

[0092]

[0093] Note: p < 0.001, significant difference "****"

[0094] Under the conditions of this experiment, as analyzed in Table 3, the inhibition rate of the essence of the present invention on elastase at a 50% test concentration is 44%, which is significantly different from the data of the blank control group (p < 0.001); it is proved that it has an inhibitory effect on elastase. According to the correlation between elastase and firming and anti-wrinkle effects, it shows that the essence of the present invention has a firming and anti-wrinkle effect.

[0095] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. An essence containing python oil for fading neck wrinkles and tightening neck skin, characterized in that, It includes the following raw materials in parts by weight: 30-35 parts of python oil, 25-30 parts of silybum marianum seed oil, 15-20 parts of jojoba seed oil, 6-10 parts of asiaticoside, 5-8 parts of ginsenoside, 4-6 parts of acetyl octapeptide-3, 4-6 parts of acetyl hexapeptide-8, 3-5 parts of coenzyme Q10, and 3-5 parts of bakuchiol.

2. The essence containing python oil according to claim 1, characterized in that, The preparation of the silybum marianum seed oil includes the following steps: S1: Select silybum marianum seeds, remove impurities, stir-fry the seeds, ultrasonically crush them, and soak them with a deep eutectic solvent to obtain silybum marianum seed slurry; S2: Purify the silybum marianum seed slurry to obtain silybum marianum seed oil I, silybum marianum seed oil, and cake residue; S3: Redissolve and extract the cake residue to obtain silybum marianum seed oil II; S4: Mix silybum marianum seed oil I and silybum marianum seed oil II to obtain the finished silybum marianum seed oil.

3. The essence containing python oil according to claim 2, characterized in that, In step S1, for the stir-frying of the seeds, first stir-fry over low heat for 5-10 min, then change to high heat and stir-fry for 8-15 min. When stir-frying over high heat, introduce steam at the bottom of the frying pan, and the introduction amount of the steam is 20-35 g / min.

4. The essence containing python oil according to claim 2, characterized in that, In step S1, the deep eutectic solvent is composed of levulinic acid and menthol with a mass ratio of 1-2:1, and the addition amount of the deep eutectic solvent is 5-10 times that of the silybum marianum seed powder.

5. The essence containing python oil according to claim 2, characterized in that, In step S1, the ultrasonic crushing frequency is 30-40 kHz, and the time is 20-40 min; the soaking temperature is 35-40 °C, and the soaking time is 4-6 h.

6. The essence containing python oil according to claim 2, characterized in that, In step S3, the cake residue is extracted by supercritical dissolution. The dissolution solution is an ethanol solution with a mass fraction of 20%-35%. The extraction pressure is 25-35 MPa, the temperature is 45-50 °C, the CO2 flow rate is 20-25 L / h, and the extraction time is 40-45 min.

7. The essence containing python oil according to claim 1, characterized in that, Its preparation steps include: Step 1: Mix asiaticoside, ginsenoside, acetyl octapeptide-3, acetyl hexapeptide-8, coenzyme Q10, and bakuchiol and add them to an ethanol solution with a mass fraction of 30%-35%. Stir, perform high-speed shearing, high-pressure homogenization, filter to remove impurities, perform high-pressure homogenization again, and concentrate to obtain mixture I; Step 2: Mix and stir python oil, silybum marianum seed oil, and jojoba seed oil, and perform high-pressure homogenization to obtain mixture II; Step 3: Mix and stir mixture I and mixture II, filter, and perform high-pressure homogenization to obtain the essence.

8. The preparation steps of the essence containing python oil according to claim 7, characterized in that, In steps 1-3, the pressure of the high-pressure homogenization is 60-75 MPa, the pressure holding time is 30-35 min, and the temperature is 15-25 °C.

9. The preparation steps of the essence containing python oil according to claim 7, characterized in that, In step 1, the rotation speed of the high-speed shearing is 7000-7500 r / min, the temperature is 25-30 °C, and the time is 5-10 min.

10. The preparation steps of the essence containing python oil according to claim 7 are characterized in that, In step 3, for the filtration, a 5-10 kDa ultrafiltration membrane is used for filtration.

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