An anti-aging composition for promoting intercellular communication of skin cells and use thereof

Abstract

The present application relates to a kind of anti-aging composition for promoting intercellular communication of skin cell and its application, the composition includes the following components: camellia japonica flower extract, bacillus fermentation product, european linden extract and safflower glucoside, the weight ratio of the camellia japonica flower extract, bacillus fermentation product, european linden extract and safflower glucoside is (1-10):(1-8):(1-6):(0.1-1).The anti-aging composition of the present application can promote the activity of skin communication cell, strengthen intercellular communication and interaction, fully exert the function of cell biology, achieve the effect of anti-aging;And inhibit the damage of cortisol to skin barrier, achieve the effect of repair and emotional stress relief, synergistic effect between components, can achieve the best wrinkle, firming, smoothing and repair effect.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cosmetics, in particular to an anti-aging composition for promoting intercellular communication of skin cells and application thereof. BACKGROUND

[0002] Dry sensitive skin is prone to problems such as water loss and excessive irritation due to weak skin barrier function, which makes it more susceptible to external environmental factors (such as climate change, pollution, etc.) and endogenous factors (such as the aging process). Studies have shown that the repair of the skin barrier is crucial for anti-aging. By using skincare products containing ingredients such as ceramides, hyaluronic acid, and squalene, it can effectively help to enhance the skin barrier and maintain skin hydration, thereby delaying the appearance of aging. Although existing anti-aging research has made progress in many aspects, there are still some limitations, mainly reflected in the following aspects:

[0003] On the one hand, most of the current anti-aging research focuses on improving skin barrier function, antioxidant and cell repair, but relatively little on intercellular communication. Intercellular communication plays an important role in the health and aging process of the skin. The skin is not only composed of different types of cells such as epidermal cells and dermal cells, but they work together through intercellular signal transmission, matrix and cell interaction, etc. to maintain the structure and function of the skin; during the aging process of the skin, the signal transmission function between cells may be impaired. With aging, the communication ability between epidermal cells and dermal cells decreases, which can lead to decreased skin hydration and reduced collagen synthesis.

[0004] On the other hand, although many anti-aging ingredients have been widely used, research on their long-term effects and safety is still insufficient. In particular, for dry sensitive skin, too many active ingredients can cause skin irritation and allergic reactions, so more clinical studies on long-term use are needed to evaluate their effectiveness and safety. SUMMARY

[0005] The purpose of the present application is to overcome the shortcomings of the prior art and provide an anti-aging composition for promoting intercellular communication of skin cells and application thereof.

[0006] To achieve the above-mentioned purpose, the technical solution adopted by the present application is as follows:

[0007] In a first aspect, the present application provides an anti-aging composition for promoting intercellular communication of skin cells, comprising the following components: Lamiophlomis rotata Bur. flower extract, Bacillus ferment, Tilia europaea L. extract and Crocus sativus glycosides, and the weight ratio of the Lamiophlomis rotata Bur. flower extract, Bacillus ferment, Tilia europaea L. extract and Crocus sativus glycosides is (1-10):(1-8):(1-6):(0.1-1).

[0008] Camellia chrysantha flower extract contains mainly flavonoids, polyphenols, and flavonoids, which have strong antioxidant effects. Its molecular structure includes the benzene ring structure of polyphenols, which can react with free radicals through its phenolic hydroxyl group, inhibit oxidation, and slow down skin aging. Secondly, the abundant polyphenol substances can promote the interaction between epidermal cells and dermal components by binding to the intercellular matrix on the skin surface, thereby enhancing the skin barrier function. Camellia chrysantha flower extract helps activate the TGF-β pathway, promotes fibroblasts in the skin to produce collagen, and improves skin elasticity through the regulation of intercellular signals, slowing down the aging process.

[0009] Bacillus fermentation product contains a large amount of protein, amino acids, and active enzymes, which can improve the repair process of the skin through enzymatic reactions. Its structure is diverse, including natural amino acid chains, peptide structures, and enzyme molecules, which can effectively activate the repair and regeneration functions of cells. Enzyme components can promote the renewal and regeneration of keratinocytes by breaking down dead skin cells on the skin surface, thereby enhancing the skin barrier function. Amino acid components improve the nutrient transfer and signal communication between cells through interaction with skin cells. Bacillus fermentation product helps to enhance the cooperation between epidermal cells and dermal layers by improving the structure of the stratum corneum, regulating cytokines such as VEGF, and promoting the microcirculation of the skin, thereby slowing down the aging process.

[0010] Tilia europaea extract contains rich flavonoids, polysaccharides, and organic acids. These components are used in skincare products to enhance the skin's antioxidant capacity, soothe skin inflammation, and promote skin repair and protection. Quercetin, as a flavonoid compound, can reduce oxidative damage to the skin by inhibiting the generation of free radicals and reducing inflammation by regulating the NF-κB pathway and the MAPK pathway. The antioxidant effect of flavonoids can reduce cell damage caused by oxidative stress during the aging process. The polysaccharide component in Tilia europaea extract can promote the interaction between skin cells, enhance skin hydration and repair function, and effectively alleviate dryness and skin problems caused by aging.

[0011] Safflower glucoside is a glycoside structure formed by the combination of glucose molecules and active ingredients in safflower, which helps it to be more easily hydrolyzed and release active ingredients in the skin, enhancing the absorption of antioxidant ingredients by skin cells and slowing down the aging process. Through flavonoid components, safflower glucoside can regulate the antioxidant pathway between cells, inhibit oxidative damage to skin cells, and improve skin firmness and elasticity.

[0012] The composition of the present application enhances the anti-oxidation and anti-aging effects of the four ingredients, i.e., the Camellia nitidissima Chang flower extract, the Bacillus ferment, the Tilia europaea extract, and the safflower glucoside, and improves the self-repairing ability and barrier function of the skin by regulating the signal transmission between cells, thereby achieving the synergistic anti-aging effect. Furthermore, the combination of the four substances can inhibit stress hormones and effectively improve the damage to the skin caused by negative emotions, repair the skin barrier, and improve the overall health of the skin.

[0013] Preferably, the weight ratio of the Camellia nitidissima Chang flower extract, the Bacillus ferment, the Tilia europaea extract, and the safflower glucoside is (1-8):(1-5):(1-4):(0.1-0.5).

[0014] Preferably, the weight ratio of the Camellia nitidissima Chang flower extract, the Bacillus ferment, the Tilia europaea extract, and the safflower glucoside is (1-5):(1-3):(1-2):(0.1-0.3).

[0015] In the second aspect, the present application provides the use of the anti-aging composition for promoting the intercellular communication of skin cells in the preparation of cosmetics.

[0016] Preferably, the cosmetic is a cosmetic water, an emulsion, a cream, a mask, an essence, or a spray, and the added amount of the composition is 1%-3% of the total weight of the cosmetic.

[0017] In the third aspect, the present application provides an essence, which comprises the following raw materials in the following weight percentages: 1%-3% of the anti-aging composition of the first aspect, 0.05%-0.5% of a thickening agent, 3%-20% of a humectant, 0.2%-5% of a preservative, 0.05%-0.1% of a pH regulator, and 0.01%-1% of a fragrance, and the balance is deionized water.

[0018] Preferably, the thickening agent comprises at least one of xanthan gum, Carbopol 980, AVC, and high-molecular cellulose.

[0019] Preferably, the humectant comprises at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, beta-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,2-butanediol, glycerol, pullulan, and ceramide.

[0020] Preferably, the pH regulator comprises at least one of arginine, sodium hydroxide, and EDTA-2Na.

[0021] Preferably, the preservative comprises at least one of 1,3-propanediol, 1,2-hexanediol, and p-hydroxyacetophenone.

[0022] In a fourth aspect, the present application provides a preparation method of the serum of the third aspect, comprising the following steps:

[0023] S1, mixing the humectant, thickening agent and part of deionized water, homogenizing at 75-85 DEG C, then adding the preservative and stirring uniformly to obtain a mixture;

[0024] S2, after the temperature of the mixture in S1 is reduced to 35-45 DEG C, adding each component of the anti-aging composition, essence and the remaining deionized water, stirring uniformly, and finally adding the pH adjuster to adjust the pH to obtain the serum.

[0025] Compared with the prior art, the present application has the following beneficial effects:

[0026] The anti-aging composition of the present application comprises four components, Camellia nitidissima flower extract, Bacillus ferment, European linden extract and safflower glucoside, which can synergistically promote the activity of skin communication cells, strengthen cell communication and interaction, fully exert the biological function of cells, achieve the effect of anti-aging, inhibit the damage of cortisol to the skin barrier, and achieve the effects of repair and emotional stress relief. The mutual synergy of the four components can achieve the best effects of wrinkle removal, tightening, smoothing and repair. BRIEF DESCRIPTION OF DRAWINGS

[0027] Figure 1 The figure is a comparison of facial wrinkles before and after using the serum of application example 1;

[0028] Figure 2 The figure is a comparison of facial wrinkles before and after using the serum of application example 5. DETAILED DESCRIPTION

[0029] In order to better illustrate the purpose, technical scheme and advantages of the present application, the present application will be further described below in combination with specific examples.

[0030] The materials used in the following examples and comparative examples are as follows:

[0031] Camellia nitidissima flower extract: manufacturer is Ximu Source (Xiamen) Biotechnology Co., Ltd.;

[0032] Bacillus ferment: manufacturer is Guangzhou Youke Biotechnology Co., Ltd.;

[0033] European linden extract: manufacturer is Haosite New Material Technology (Suzhou) Co., Ltd.;

[0034] Safflower glucoside: manufacturer is Jingchu Tiancheng Pharmaceutical Technology Co., Ltd.

[0035] Example 1

[0036] An anti-aging composition for promoting intercellular communication of skin cells, comprising Camellia japonica flower extract, Bacillus ferment, Tilia europaea wood extract, and Crocus sativus glucoside in a weight ratio of 4:2:1.3:0.2, the total mass fraction of the Camellia japonica flower extract, the Bacillus ferment, the Tilia europaea wood extract, and the Crocus sativus glucoside being 100 parts.

[0037] Example 2

[0038] An anti-aging composition for promoting intercellular communication of skin cells, comprising Camellia japonica flower extract, Bacillus ferment, Tilia europaea wood extract, and Crocus sativus glucoside in a weight ratio of 1:3:2:0.3, the total mass fraction of the Camellia japonica flower extract, the Bacillus ferment, the Tilia europaea wood extract, and the Crocus sativus glucoside being 100 parts.

[0039] Example 3

[0040] An anti-aging composition for promoting intercellular communication of skin cells, comprising Camellia japonica flower extract, Bacillus ferment, Tilia europaea wood extract, and Crocus sativus glucoside in a weight ratio of 5:1:1:0.1, the total mass fraction of the Camellia japonica flower extract, the Bacillus ferment, the Tilia europaea wood extract, and the Crocus sativus glucoside being 100 parts.

[0041] Example 4

[0042] An anti-aging composition for promoting intercellular communication of skin cells, comprising Camellia japonica flower extract, Bacillus ferment, Tilia europaea wood extract, and Crocus sativus glucoside in a weight ratio of 1:1:4:0.5, the total mass fraction of the Camellia japonica flower extract, the Bacillus ferment, the Tilia europaea wood extract, and the Crocus sativus glucoside being 100 parts.

[0043] Example 5

[0044] An anti-aging composition for promoting intercellular communication of skin cells, comprising Camellia japonica flower extract, Bacillus ferment, Tilia europaea wood extract, and Crocus sativus glucoside in a weight ratio of 8:5:1:0.1, the total mass fraction of the Camellia japonica flower extract, the Bacillus ferment, the Tilia europaea wood extract, and the Crocus sativus glucoside being 100 parts.

[0045] Example 6

[0046] An anti-aging composition for promoting intercellular communication of skin cells, comprising Camellia japonica flower extract, Bacillus ferment, Tilia europaea wood extract, and Crocus sativus glucoside in a weight ratio of 1:8:6:0.1, the total mass fraction of the Camellia japonica flower extract, the Bacillus ferment, the Tilia europaea wood extract, and the Crocus sativus glucoside being 100 parts.

[0047] Example 7

[0048] An anti-aging composition for promoting intercellular communication of skin cells, comprising Camellia nitidissima H.T. Chang flower extract, Bacillus ferment, Tilia europaea L. extract, and Crocus sativus glycosides in a weight ratio of 10:1:0.1:0.01, wherein the total mass fraction of the Camellia nitidissima H.T. Chang flower extract, the Bacillus ferment, the Tilia europaea L. extract, and the Crocus sativus glycosides is 100 parts.

[0049] Comparative Example 1

[0050] Comparative Example 1 differs from Example 1 in that the composition does not add Camellia nitidissima H.T. Chang flower extract, and the missing amount is supplemented with Bacillus ferment, Tilia europaea L. extract, and Crocus sativus glycosides in a mass ratio of 2:1.3:0.2.

[0051] Comparative Example 2

[0052] Comparative Example 2 differs from Example 1 in that the composition does not add Bacillus ferment, and the missing amount is supplemented with Camellia nitidissima H.T. Chang flower extract, Tilia europaea L. extract, and Crocus sativus glycosides in a weight ratio of 4:1.3:0.2.

[0053] Comparative Example 3

[0054] Comparative Example 3 differs from Example 1 in that the composition does not add Tilia europaea L. extract, and the missing amount is supplemented with Camellia nitidissima H.T. Chang flower extract, Bacillus ferment, and Crocus sativus glycosides in a weight ratio of 4:2:0.2.

[0055] Comparative Example 4

[0056] Comparative Example 4 differs from Example 1 in that the composition does not add Crocus sativus glycosides, and the missing amount is supplemented with Camellia nitidissima H.T. Chang flower extract, Bacillus ferment, and Tilia europaea L. extract in a weight ratio of 4:2:1.3.

[0057] Test Example 1: Evaluation of the effect of the sample on the activity of human skin fibroblasts (HSF) based on the MTT method

[0058] 1. Materials and reagents

[0059] Cell line: Human skin fibroblasts (HSF), purchased from the Kunming Cell Bank of the Chinese Academy of Sciences.

[0060] Culture medium: High-sugar DMEM culture medium (1x, containing 10% fetal bovine serum FBS, Gibco).

[0061] Reagents: Phosphate buffer (PBS, Shanghai Solaybao), thiazolyl blue (MTT, Yuanye Bio), dimethyl sulfoxide (DMSO, Tianjin Damao).

[0062] Test samples: Anti-aging compositions of Examples 1-7 and Comparative Examples 1-4, and the compositions were configured into test sample solutions with a concentration of 0.05% (m / m) using fresh culture medium.

[0063] 2. Experimental procedures

[0064] 2.1 Cell seeding and culture

[0065] The HSF cell suspension was seeded in a 96-well cell culture plate at a seeding density of about 5 x 103cells per well (the specific density needs to be optimized according to the cell growth rate). The culture plate was placed in a 37°C, 5% CO2incubator for 18-24 h until the cells adhered.

[0066] 2.2 Sample treatment

[0067] The original culture medium in the wells was discarded, and the cells were gently washed once with PBS along the well wall to avoid cell shedding.

[0068] Experimental grouping:

[0069] Sample group: 100 μL of test sample solution with a concentration of 0.05% (m / m) was added to each well.

[0070] Blank control group: 100 μL of fresh culture medium (without test sample) was added to each well.

[0071] 2.3 MTT detection of cell viability

[0072] The culture plates containing the sample group and the blank control group were returned to the incubator for 24±1 h. After incubation, 20 μL of MTT solution (5 mg / mL, prepared with PBS) was added to each well, and the mixture was gently mixed. The culture was continued for 3-4 h to allow the living cells to reduce MTT to formazan crystals. The liquid in the wells was carefully aspirated to avoid aspirating the formazan crystals, 100 μL of DMSO was added to each well, and the plate was placed on a shaker for 10-15 min to fully dissolve the crystals.

[0073] 2.4 Absorbance determination and calculation

[0074] The absorbance (OD value) of each well was determined at a wavelength of 570 nm using a microplate reader, and the cell-free wells (containing only culture medium and MTT) were set as the zero wells.

[0075] HSF cell viability calculation formula:

[0076] HSF cell viability = (OD value 样品孔 - OD value 调零 ) / (OD value 空白对照孔 - OD value 调零Cell viability data of HSF cells reflect the proliferation status of HSF cells. Higher cell viability indicates that the cells have stronger proliferative capacity and can grow and divide rapidly, which is essential for maintaining the normal renewal and repair functions of skin tissue. This is because HSF cells have strong collagen and elastin synthesis capacity, promoting wound healing and skin elasticity through proliferation and tissue repair. See Table 1 for specific data.

[0077] Test Example 2: Evaluation of the improvement of samples on cortisol-induced HaCaT cell damage based on MTT method

[0078] 1. Experimental materials

[0079] Phosphate buffer solution (PBS, Shanghai Solaybao), thiazolyl blue (MTT, source leaf biological), dimethyl sulfoxide (DMSO, Tianjin Damao), high-sugar culture medium 1xDMEM (Gibco), fetal bovine serum (FBS, Gibco), human keratinocyte HaCaT (Shanghai Yaji Biological Technology Co., Ltd.), cortisol (MedChemExpress company, HY-N0583).

[0080] 2. Experimental conditions

[0081] Incubator temperature: 37±1℃; humidity: 90±5%; carbon dioxide concentration: 5±1%.

[0082] 3. Experimental steps

[0083] 3.1 Cell inoculation: human keratinocyte HaCaT suspension was inoculated in a 96-well cell culture plate at a density of 2000 cells per well.

[0084] 3.2 Culture medium addition: add 100 μL DMEM medium (containing 10% fetal bovine serum and 1% penicillin / streptomycin) per well, and incubate for 24 hours.

[0085] 3.3 Group treatment:

[0086] Blank group: after discarding the supernatant, add 100 μL DMEM medium.

[0087] Model group: add 100 μL DMEM medium containing 100 nM cortisol.

[0088] Sample group: add 100 μL medium containing 100 nM cortisol and 0.05% (m / m) corresponding sample liquid; the sample liquid corresponds to the anti-aging composition of Examples 1-7 and Comparative Examples 1-4, respectively.

[0089] 4. Incubation and detection: After 24 hours of incubation, 20 μL of MTT solution was added to each well, and the plate was incubated in the incubator for 3-4 hours.

[0090] 5. Absorbance determination: The liquid in the wells was removed, 100 μL of DMSO was added to each well, and after shaking for 10-15 minutes, the absorbance OD value was determined at a wavelength of 570 nm on an enzyme-labeled instrument.

[0091] 6. Cell viability calculation: The HaCaT cell viability and the HaCaT cell viability improvement rate were calculated according to the formula:

[0092] HaCaT cell viability (%) = (OD value of sample well - OD value of zero) / (OD value of blank control well - OD value of zero) x 100%.

[0093] HaCaT cell viability improvement rate (%) = (cell viability of sample group - cell viability of model group) / cell viability of model group x 100%. The improvement rate is used to evaluate the repair effect of the sample on the cortisol damage, and a positive value indicates improvement, and a negative value indicates aggravation of damage. The cell viability improvement rate reflects the repair ability of the sample on the cortisol-induced HaCaT cell damage. A higher improvement rate means that the sample can more effectively reduce the damage to the cells caused by cortisol, promote the recovery of normal function and viability of the cells, and thus protect the skin cells from stress damage to a certain extent. See Table 1 for details.

[0094] Table 1 Test data of samples in each group

[0095] Group Fibroblast viability (%) Keratinocyte cell viability improvement rate (%) Example 1 120.4 62.7 Example 2 116.1 60.9 Example 3 115.7 60.7 Example 4 112.0 58.5 Example 5 113.6 58.7 Example 6 104.1 55.8 Example 7 103.6 55.5 Comparative Example 1 87.1 17.4 Comparative Example 2 81.6 18.7 Comparative Example 3 83.7 18.9 Comparative Example 4 87.5 19.2

[0096] As can be seen from Table 1, in combination with the data of Example 1 and Comparative Examples 1-4, it can be seen that the composition of Example 1, which is a combination of Camellia nitidissima flower extract, Bacillus ferment, European linden wood extract, and safflower glucoside, has a significant improvement in the effects on fibroblast cell viability and keratinocyte cell viability improvement rate compared to the compositions of Comparative Examples 1-4. This indicates that the four ingredients of Camellia nitidissima flower extract, Bacillus ferment, European linden wood extract, and safflower glucoside can synergistically promote the proliferation of HSF cells and improve the repair ability of the sample on cortisol-induced HaCaT cell damage. This may be because the four ingredients improve the communication and interaction between skin cells, thereby improving the viability of HSF cells, overall improving skin health, achieving the effects of lifting, tightening, and filling, and inhibiting the damage of stress hormones to the skin barrier, and improving the effects of emotional stress hormones on the skin barrier.

[0097] In combination with the data of Examples 1-7, when the weight ratio of Camellia japonica flower extract, Bacillus fermentation product, Tilia europaea extract and safflower glucoside is (1-5):(1-3):(1-2):(0.1-0.3), the composition has a better effect on improving the activity of fibroblasts and the activity improvement rate of keratinocytes.

[0098] Application Examples 1-7 and Comparative Application Examples 1-4

[0099] The compositions of Examples 1-7 and Comparative Examples 1-4 are respectively added to the serum at a concentration of 3wt%, to obtain the serum of Application Examples 1-7 and Comparative Application Examples 1-4, and the formula is shown in Table 2.

[0100] The preparation method of the serum of Application Examples 1-7 and Comparative Application Examples 1-4 comprises the following steps:

[0101] The preparation method of the serum comprises the following steps:

[0102] S1, mix the humectant, thickening agent and 1 / 2 amount of deionized water, homogenize at 80°C, then add the preservative and stir until uniform, to obtain a mixture;

[0103] S2, after the temperature of the mixture in S1 drops to 40°C, add the components of the anti-aging composition, essence and the remaining deionized water, stir until uniform, and finally add the pH adjuster to adjust the pH to 6, to obtain the serum.

[0104] Table 2 Formulas of the serum of Application Examples 1-7 and Comparative Application Examples 1-4

[0105]

[0106] Comparative Application Example 5

[0107] The serum of Comparative Application Example 5 does not add the anti-aging composition, and an equal amount of deionized water is used instead of the composition, and the preparation method is the same as that of Application Example 1.

[0108] Test Example 3: Efficacy test of serum

[0109] This test example verifies the effects of the serum of Application Examples 1-7 and Comparative Application Examples 1-5 on anti-aging and barrier repair, and the experimental data is shown in Table 3.

[0110] According to the test of the Cosmetics Safety Technical Standard (2015), 60 volunteers with dry and sensitive skin aged 18-45 years (lactic acid stinging score > 3, skin moisture content 20 C.U~50 C.U) were randomly divided into 12 groups, 5 people in each group. Under normal circumstances, the volunteers used the product on the whole face, and the volunteers took 1 mL of the essence and applied it to the face, and then massaged and absorbed it. The volunteers were in an air-conditioned room with a temperature of 21±1℃ and a humidity of 50±10%, and the instrument was used to quantify and take pictures of the improvement of the facial indicators. The test time was the indicators before use, 7 days after use, and 28 days after use. Primos CR 5.8E three-dimensional skin analyzer was used to test wrinkles, Cutometer 2mm MPA 580 skin elasticity tester was used to test elasticity, and Tewameter TM Hex transdermal water loss instrument was used to measure the transdermal water loss TEWL value.

[0111] The calculation formula is as follows:

[0112] Wrinkle improvement rate = (wrinkle 使用前 -wrinkle 使用后 ) / wrinkle 使用前 ×100%;

[0113] Elasticity improvement rate = (elasticity 使用后 -elasticity 使用前 ) / elasticity 使用前 ×100%.

[0114] TEWL value improvement rate = (TEWL value 使用后 -TEWL value 使用前 ) / TEWL value 使用前 ×100%;

[0115] The calculation results are shown in Table 3.

[0116] Table 3 Human efficacy test data of essence samples in each group

[0117]

[0118] The effect of the application example against aging is represented by the wrinkle improvement rate and the elasticity improvement rate, and the repair effect on barrier damage is represented by the TEWL value improvement rate.

[0119] From Figures 1-2 It can be seen that the wrinkle improvement effect of the essence of the application example 1 on the subjects after 28 days of use is significantly improved compared with the comparative application example 5, which shows that the essence added with the anti-aging composition has good anti-aging and repair effects.

[0120] As can be seen from the data of application example 1 and comparative application examples 1-4 in Table 2, the composition of example 1 selected from four components of camellia japonica flower extract, bacillus fermentation product, tilia europaea extract and crocosmos glycosides has a significantly improved effect of anti-aging and barrier repair compared with the compositions of comparative examples 1-4, which indicates that the four components of camellia japonica flower extract, bacillus fermentation product, tilia europaea extract and crocosmos glycosides can synergistically play the effects of barrier repair and anti-aging.

[0121] As can be seen from the data of application examples 1-7, when the weight ratio of camellia japonica flower extract, bacillus fermentation product, tilia europaea extract and crocosmos glycosides is (1-5):(1-3):(1-2):(0.1-0.3), the composition has a better effect of barrier repair and anti-aging.

[0122] In summary, the anti-aging composition of the present application enhances the effects of antioxidant and anti-aging of the composition through the synergistic effect of the four components of camellia japonica flower extract, bacillus fermentation product, tilia europaea extract and crocosmos glycosides, and improves the self-repairing ability and barrier function of the skin by regulating the signal transmission between cells, so as to realize the synergistic anti-aging effect, and can also inhibit stress hormones and effectively improve the damage to the skin caused by negative emotions, repair the barrier, and improve the overall health of the skin.

[0123] Finally, it should be noted that the above examples are only used to illustrate the technical solutions of the present application and not to limit the protection scope of the present application. Although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application can be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present application.

Claims

1. An anti-aging composition that promotes intercellular communication in skin cells, characterized in that, It includes the following components: Camellia chrysantha flower extract, Bacillus fermentation product, European linden extract and safflower glucoside, wherein the weight ratio of Camellia chrysantha flower extract, Bacillus fermentation product, European linden extract and safflower glucoside is (1-10):(1-8):(1-6):(0.1-1).

2. The anti-aging composition for promoting intercellular communication in skin cells as described in claim 1, characterized in that, The weight ratio of the Camellia chrysantha flower extract, Bacillus fermentation product, Tilia amurensis extract and safflower glucoside is (1-8):(1-5):(1-4):(0.1-0.5).

3. The anti-aging composition for promoting intercellular communication in skin cells as described in claim 1, characterized in that, The weight ratio of the Camellia chrysantha flower extract, Bacillus fermentation product, Tilia amurensis extract and safflower glucoside is (1-5):(1-3):(1-2):(0.1-0.3).

4. The use of the anti-aging composition for promoting intercellular communication of skin cells according to any one of claims 1-3 in the preparation of cosmetics.

5. The application of the anti-aging composition for promoting intercellular communication in skin cells as described in claim 4 in the preparation of cosmetics, characterized in that, The cosmetic is a toner, lotion, cream, mask, serum, or spray, and the amount of the composition added is 1%-3% of the total weight of the cosmetic.

6. An essence, characterized in that, The ingredients comprise the following ingredients by weight percentage: 1%-3% of the anti-aging composition according to any one of claims 1-3, 0.05%-0.5% of thickener, 3%-20% of humectant, 0.2%-5% of preservative, 0.05%-0.1% of pH adjuster, and 0.01%-1% of fragrance, with the balance being deionized water.

7. The essence as described in claim 6, characterized in that, The raw material is selected from at least one of (a)-(d): (a) The thickener includes at least one of xanthan gum, carbomer 980, AVC, and high molecular weight cellulose; (b) The moisturizer comprises at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,2-butanediol, glycerin, budding stalk polysaccharide and ceramide; (c) The pH adjuster includes at least one of arginine, sodium hydroxide and EDTA-2Na; (d) The preservative includes at least one of 1,3-propanediol, 1,2-hexanediol and p-hydroxyacetophenone.

8. The method for preparing the essence according to claim 7, characterized in that, Includes the following steps: S1. Mix the humectant, thickener and part of the deionized water, homogenize at 75-85℃, then add the preservative and stir evenly to obtain the mixture. S2. When the temperature of the mixture in S1 drops to 35-45℃, add the components of the anti-aging composition, fragrance and remaining deionized water, stir evenly, and finally add pH adjuster to adjust pH to obtain the essence.

Images