An ethanol-free patch of gynura japonica having an anti-depression effect and a preparation method thereof

Abstract

The application discloses an ethanol-free patchouli incense sheet with an anti-depression effect, which is prepared from the following raw materials in parts by weight: patchouli 30-50 parts, rose 10-20 parts, ambrox 5-15 parts and amber 5-10 parts. The anti-depression function of the incense sheet is realized through the synergistic effect of patchouli and other natural fragrances. Research shows that patchouli leaves are rich in 2-acetyl-1-pyrroline (2-AP) and beta-caryophyllene active ingredients, which can stimulate the brain limbic system through the olfactory pathway, promote the release of 5-hydroxytryptamine (5-HT) and dopamine (DA), and significantly improve the emotional state. In addition, the linalool in the rose and the ambrox in the ambrox form a synergistic effect, activate the prefrontal cortex 5-HT1A receptor, enhance the alpha brain wave power spectrum density, and realize rapid emotional regulation. The ethanol-free patchouli incense sheet formula avoids the side effects of traditional antidepressants, provides a safe and convenient emotional intervention scheme through natural aromatherapy, and meets the non-drug mental health management trend advocated by the WHO.

Description

Technical Field

[0001] The present invention relates to the technical field of ethanol-free pandan-flavored tablets, in particular to ethanol-free pandan-flavored tablets with antidepressant efficacy and a preparation method thereof. Background Art

[0002] Aromatherapy tablets are a type of fragrance product that can be used to improve the air quality in the car and add a comfortable atmosphere, slowly releasing the fragrance through the air flow.

[0003] (1) Traditional scented tablets mostly rely on ethanol as a solvent, which can easily cause skin irritation and a pungent odor. In addition, pandan scented tablets containing ethanol are highly flammable and are prohibited from being carried in cars, railways, and airlines. They can cause fires and explosions when exposed to open flames.

[0004] (2) Nowadays, social pressure is high and more and more people are suffering from depression. Existing scented tablets do not have the function of anti-depression. Summary of the Invention

[0005] The object of the present invention is to provide an ethanol-free pandan-scented tablet with antidepressant efficacy and a preparation method thereof, so as to solve the problems raised in the above-mentioned background technology.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] The invention discloses an ethanol-free pandan scented tablet with antidepressant effect, comprising the following raw materials in parts by weight: 30-50 parts of pandan, 10-20 parts of rose, 5-15 parts of ambergris, and 5-10 parts of amber.

[0008] As a further solution of the present invention: the raw materials include the following in parts by weight: 40-50 parts of pandan, 10-18 parts of rose, 7-15 parts of ambergris, and 6-10 parts of amber.

[0009] As a further solution of the present invention: comprising the following raw materials in parts by weight: 45 parts of pandan, 15 parts of rose, 10 parts of ambergris, and 8 parts of amber.

[0010] A method for preparing ethanol-free pandan-flavored tablets with antidepressant efficacy comprises the following steps:

[0011] S1: fresh pandan leaves were selected by weight and extracted with supercritical carbon dioxide to obtain a green oil;

[0012] S2: Weigh rose, ambergris, and amber by weight and crush them into a powder with a particle size of 100 mesh;

[0013] S3: Mix the green oil obtained in S1 with the powder obtained in S2 and stir until homogenized;

[0014] S4: Inject into the mold, cure at room temperature for 24 hours, cut into 5cm×5cm scented slices, and seal in aluminum foil bags to protect from light.

[0015] As a further solution of the present invention: in S1, the critical temperature of carbon dioxide in supercritical carbon dioxide extraction is 45°C, the critical pressure is 25 MPa, and the extraction time is 2 hours.

[0016] As a further solution of the present invention: in S3, stirring and homogenizing is carried out at 25-30°C, with a stirring speed of 200-300 rpm and a duration of 30-60 minutes.

[0017] As a further solution of the present invention: in S4, room temperature curing is carried out under the condition of relative humidity of 40%-60%.

[0018] As a further solution of the present invention: in S4, the thickness of the scented sheet is 2-5 mm.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] The present invention realizes the antidepressant function of scented tablets through the synergistic effect of pandan and other natural spices. Studies have shown that pandan leaves are rich in active ingredients such as 2-acetyl-1-pyrroline (2-AP) and β-caryophyllene, which can stimulate the brain's limbic system through the olfactory pathway, promote the release of serotonin (5-HT) and dopamine (DA), and significantly improve the emotional state. In addition, the linalool of the rose and the ambroxan of ambergris in the formula form a synergistic effect, activate the 5-HT1A receptors in the prefrontal cortex, enhance the alpha brain wave power spectral density, and achieve rapid emotional regulation. The ethanol-free pandan scented tablet formula of the present invention avoids the side effects of traditional antidepressants (such as drowsiness and dry mouth), provides a safe and convenient emotional intervention program through natural aromatherapy, and is in line with the trend of non-drug mental health management advocated by the WHO. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 The present invention is a process flow chart of the preparation method of ethanol-free pandan-flavored tablets with antidepressant effect. DETAILED DESCRIPTION

[0022] Various exemplary embodiments, features, and aspects of the present application will be described in detail below with reference to the accompanying drawings. The same reference numerals in the accompanying drawings represent elements with the same or similar functions. Although various aspects of the embodiments are shown in the accompanying drawings, the drawings are not necessarily drawn to scale unless otherwise indicated.

[0023] The word “exemplary” is used exclusively herein to mean “serving as an example, example, or illustration.” Any embodiment described herein as “exemplary” is not necessarily to be construed as preferred or advantageous over other embodiments.

[0024] In addition, numerous specific details are provided in the following specific examples to better illustrate the present application. Those skilled in the art will appreciate that the present application can be practiced without certain specific details. In some instances, methods, means, and components well known to those skilled in the art are not described in detail in order to highlight the main purpose of the present application.

[0025] Example 1

[0026] Disclosed are ethanol-free pandan scented tablets with antidepressant efficacy, comprising the following raw materials in parts by weight: 30 parts of pandan, 10 parts of rose, 5 parts of ambergris, and 5 parts of amber.

[0027] A method for preparing ethanol-free pandan-flavored tablets with antidepressant efficacy comprises the following steps:

[0028] S1: fresh pandan leaves were selected by weight and extracted with supercritical carbon dioxide to obtain a green oil;

[0029] S2: Weigh rose, ambergris, and amber by weight and crush them into a powder with a particle size of 100 mesh;

[0030] S3: Mix the green oil obtained in S1 with the powder obtained in S2 and stir until homogenized;

[0031] S4: Inject into the mold, cure at room temperature for 24 hours, cut into 5cm×5cm scented slices, and seal in aluminum foil bags to protect from light.

[0032] Preferably, in this embodiment, in S1, the critical temperature of carbon dioxide in supercritical carbon dioxide extraction is 45° C., the critical pressure is 25 MPa, and the extraction time is 2 hours.

[0033] Preferably, in this embodiment, in S3, stirring and homogenizing is performed at 25-30°C, with a stirring speed of 200-300 rpm and a duration of 30-60 minutes.

[0034] Preferably, in this embodiment, in S4 , room temperature curing is performed under a relative humidity of 40%-60%.

[0035] Preferably, in this embodiment, in S4, the thickness of the scented sheet is 2-5 mm.

[0036] Example 2

[0037] The invention discloses an ethanol-free pandan scented tablet with antidepressant effect, comprising the following raw materials in parts by weight: 50 parts of pandan, 20 parts of rose, 15 parts of ambergris, and 10 parts of amber.

[0038] A method for preparing ethanol-free pandan-flavored tablets with antidepressant efficacy comprises the following steps:

[0039] S1: fresh pandan leaves were selected by weight and extracted with supercritical carbon dioxide to obtain a green oil;

[0040] S2: Weigh rose, ambergris, and amber by weight and crush them into a powder with a particle size of 100 mesh;

[0041] S3: Mix the green oil obtained in S1 with the powder obtained in S2 and stir until homogenized;

[0042] S4: Inject into the mold, cure at room temperature for 24 hours, cut into 5cm×5cm scented slices, and seal in aluminum foil bags to protect from light.

[0043] Preferably, in this embodiment, in S1, the critical temperature of carbon dioxide in supercritical carbon dioxide extraction is 45° C., the critical pressure is 25 MPa, and the extraction time is 2 hours.

[0044] Preferably, in this embodiment, in S3, stirring and homogenizing is performed at 25-30°C, with a stirring speed of 200-300 rpm and a duration of 30-60 minutes.

[0045] Preferably, in this embodiment, in S4 , room temperature curing is performed under a relative humidity of 40%-60%.

[0046] Preferably, in this embodiment, in S4, the thickness of the scented sheet is 2-5 mm.

[0047] Example 3

[0048] Disclosed are ethanol-free pandan scented tablets with antidepressant efficacy, comprising the following raw materials in parts by weight: 40 parts of pandan, 10 parts of rose, 7 parts of ambergris, and 6 parts of amber.

[0049] A method for preparing ethanol-free pandan-flavored tablets with antidepressant efficacy comprises the following steps:

[0050] S1: fresh pandan leaves were selected by weight and extracted with supercritical carbon dioxide to obtain a green oil;

[0051] S2: Weigh rose, ambergris, and amber by weight and crush them into a powder with a particle size of 100 mesh;

[0052] S3: Mix the green oil obtained in S1 with the powder obtained in S2 and stir until homogenized;

[0053] S4: Inject into the mold, cure at room temperature for 24 hours, cut into 5cm×5cm scented slices, and seal in aluminum foil bags to protect from light.

[0054] Preferably, in this embodiment, in S1, the critical temperature of carbon dioxide in supercritical carbon dioxide extraction is 45° C., the critical pressure is 25 MPa, and the extraction time is 2 hours.

[0055] Preferably, in this embodiment, in S3, stirring and homogenizing is performed at 25-30°C, with a stirring speed of 200-300 rpm and a duration of 30-60 minutes.

[0056] Preferably, in this embodiment, in S4 , room temperature curing is performed under a relative humidity of 40%-60%.

[0057] Preferably, in this embodiment, in S4, the thickness of the scented sheet is 2-5 mm.

[0058] Example 4

[0059] The invention discloses an ethanol-free pandan scented tablet with antidepressant effect, comprising the following raw materials in parts by weight: 50 parts of pandan, 18 parts of rose, 15 parts of ambergris, and 10 parts of amber.

[0060] A method for preparing ethanol-free pandan-flavored tablets with antidepressant efficacy comprises the following steps:

[0061] S1: fresh pandan leaves were selected by weight and extracted with supercritical carbon dioxide to obtain a green oil;

[0062] S2: Weigh rose, ambergris, and amber by weight and crush them into a powder with a particle size of 100 mesh;

[0063] S3: Mix the green oil obtained in S1 with the powder obtained in S2 and stir until homogenized;

[0064] S4: Inject into the mold, cure at room temperature for 24 hours, cut into 5cm×5cm scented slices, and seal in aluminum foil bags to protect from light.

[0065] Preferably, in this embodiment, in S1, the critical temperature of carbon dioxide in supercritical carbon dioxide extraction is 45° C., the critical pressure is 25 MPa, and the extraction time is 2 hours.

[0066] Preferably, in this embodiment, in S3, stirring and homogenizing is performed at 25-30°C, with a stirring speed of 200-300 rpm and a duration of 30-60 minutes.

[0067] Preferably, in this embodiment, in S4 , room temperature curing is performed under a relative humidity of 40%-60%.

[0068] Preferably, in this embodiment, in S4, the thickness of the scented sheet is 2-5 mm.

[0069] Example 5

[0070] The invention discloses an ethanol-free pandan scented tablet with antidepressant effect, comprising the following raw materials in parts by weight: 45 parts of pandan, 15 parts of rose, 10 parts of ambergris, and 8 parts of amber.

[0071] A method for preparing ethanol-free pandan-flavored tablets with antidepressant efficacy comprises the following steps:

[0072] S1: fresh pandan leaves were selected by weight and extracted with supercritical carbon dioxide to obtain a green oil;

[0073] S2: Weigh rose, ambergris, and amber by weight and crush them into a powder with a particle size of 100 mesh;

[0074] S3: Mix the green oil obtained in S1 with the powder obtained in S2 and stir until homogenized;

[0075] S4: Inject into the mold, cure at room temperature for 24 hours, cut into 5cm×5cm scented slices, and seal in aluminum foil bags to protect from light.

[0076] Preferably, in this embodiment, in S1, the critical temperature of carbon dioxide in supercritical carbon dioxide extraction is 45° C., the critical pressure is 25 MPa, and the extraction time is 2 hours.

[0077] Preferably, in this embodiment, in S3, stirring and homogenizing is performed at 25-30°C, with a stirring speed of 200-300 rpm and a duration of 30-60 minutes.

[0078] Preferably, in this embodiment, in S4 , room temperature curing is performed under a relative humidity of 40%-60%.

[0079] Preferably, in this embodiment, in S4, the thickness of the scented sheet is 2-5 mm.

[0080] Experiment 1: Active ingredient release verification

[0081] Gas chromatography-mass spectrometry was used to monitor the properties of the scented tablets at 25°C;

[0082]

[0083]

[0084] Experiment 2: Animal behavior test (mouse forced swimming test)

[0085] **p<0.01VS control group; exposure conditions: closed space (1m 3 ) Hanging scented sheets for 2 hours per day for 14 days;

[0086] Group Immobility time (s) 5-HT level (ng / mL) DA level (pg / mL) control group 188±21 32.4±3.1 415±38 Fragrant tablets exposure group 102±15** 58.7±4.5** 692±52** Fluoxetine group 89±12 62.1±5.2 705±49

[0087] The data show that the antidepressant effect of the present invention is comparable to that of fluoxetine, and has no side effects of weight loss or activity inhibition.

[0088] Experiment 3: Neural Receptor Specificity (In Vitro CHO Cell Model)

[0089] Calcium ion fluorescence labeling method, the concentration gradient of balsam pear extract was 0.1-10 μg / mL;

[0090] Receptor type Activation rate (vs control group) EC50 (μg / mL) 5-HT1A 182％±15％** 0.32 5-HT2A 105％±8％ ＞10 D2 123％±9％* 1.78

[0091] The data show that the present invention can activate the 5-HT1A receptor in the prefrontal cortex, enhance the power spectrum density of alpha brain waves, and achieve rapid emotion regulation.

[0092] Experiment 4: Human Trial

[0093] Participants: 60 patients with mild to moderate depression (BDI-II score ≥ 14) were divided into two groups.

[0094] Intervention: The experimental group used scented tablets for 2 hours daily, while the control group used inactive scented tablets.

[0095] Results (after 4 weeks):

[0096]

[0097]

[0098] *p<0.05, **p<0.01, experimental data show that the present invention is safe and effective.

[0099] The present invention realizes the antidepressant function of scented tablets through the synergistic effect of pandan and other natural spices. Studies have shown that pandan leaves are rich in active ingredients such as 2-acetyl-1-pyrroline (2-AP) and β-caryophyllene, which can stimulate the brain's limbic system through the olfactory pathway, promote the release of serotonin (5-HT) and dopamine (DA), and significantly improve the emotional state. In addition, the linalool of the rose and the ambroxan of ambergris in the formula form a synergistic effect, activate the 5-HT1A receptors in the prefrontal cortex, enhance the alpha brain wave power spectral density, and achieve rapid emotional regulation. The ethanol-free pandan scented tablet formula of the present invention avoids the side effects of traditional antidepressants (such as drowsiness and dry mouth), provides a safe and convenient emotional intervention program through natural aromatherapy, and is in line with the trend of non-drug mental health management advocated by the WHO.

[0100] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the invention can be embodied in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, the embodiments should be considered in all respects as illustrative and non-restrictive, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations that come within the meaning and range of equivalents of the claims be embraced therein.

[0101] In addition, it should be understood that although this specification is described in terms of implementation methods, not every implementation method contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.

Claims

1. An ethanol-free pandan-flavored tablet with antidepressant effect, characterized in that: The invention is prepared from the following raw materials in parts by weight: 30-50 parts of pandanus, 10-20 parts of rose, 5-15 parts of ambergris, and 5-10 parts of amber; A method for preparing ethanol-free pandan-flavored tablets with antidepressant efficacy, The following steps are involved: S1: fresh pandan leaves were selected by weight and extracted with supercritical carbon dioxide to obtain a green oil; S2: Weigh rose, ambergris, and amber by weight and crush them into a powder with a particle size of 100 mesh; S3: Mix the green oil obtained in S1 with the powder obtained in S2 and stir until homogenized; S4: Inject into the mold, cure at room temperature for 24 hours, cut into 5cm×5cm scented slices, and seal in aluminum foil bags to protect from light.

2. The ethanol-free pandan-flavored tablets with antidepressant efficacy according to claim 1, characterized in that: The invention is prepared from the following raw materials in parts by weight: 40-50 parts of pandan, 10-18 parts of rose, 7-15 parts of ambergris and 6-10 parts of amber.

3. The ethanol-free pandan-flavored tablets with antidepressant efficacy according to claim 1, characterized in that: The invention is prepared from the following raw materials in parts by weight: 45 parts of pandan, 15 parts of rose, 10 parts of ambergris and 8 parts of amber.

4. A method for preparing the ethanol-free pandan-flavored tablets with antidepressant efficacy according to any one of claims 1 to 3, characterized in that: The following steps are involved: S1: fresh pandan leaves were selected by weight and extracted with supercritical carbon dioxide to obtain a green oil; S2: Weigh rose, ambergris, and amber by weight and crush them into a powder with a particle size of 100 mesh; S3: Mix the green oil obtained in S1 with the powder obtained in S2 and stir until homogenized; S4: Inject into the mold, cure at room temperature for 24 hours, cut into 5cm×5cm scented slices, and seal in aluminum foil bags to protect from light.

5. The method for preparing ethanol-free pandan-flavored tablets with antidepressant efficacy according to claim 4, characterized in that: In S1, the critical temperature of carbon dioxide in supercritical carbon dioxide extraction is 45°C, the critical pressure is 25 MPa, and the extraction time is 2 hours.

6. The method for preparing the ethanol-free pandan-flavored tablets with antidepressant efficacy according to claim 4, characterized in that: In S3, stirring homogenization is performed at 25-30°C, at a stirring speed of 200-300 rpm, and for a duration of 30-60 minutes.

7. The method for preparing ethanol-free pandan-flavored tablets with antidepressant efficacy according to claim 4, characterized in that: In S4, room temperature curing is carried out at a relative humidity of 40%-60%.

8. The method for preparing ethanol-free pandan-flavored tablets with antidepressant efficacy according to claim 4, characterized in that: In S4, the thickness of the scented sheet is 2-5 mm.

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