Plant growth regulator containing oil tea dregs and preparation method thereof

Abstract

The present application belongs to the field of plant growth regulators, and particularly relates to a plant growth regulator containing camellia oleifera dreg and a preparation method thereof. The plant growth regulator is prepared by using waste camellia oleifera dreg, lentinus edodes dreg, vinasse, sugar cane dreg and banana stem and leaf as fermentation substrates, adding Trichoderma harzianum and yeast for fermentation, and then adding livestock and poultry slaughter waste and Bacillus amyloliquefaciens for fermentation treatment. Through the fermentation culture in stages, the different microorganisms and substrates interact with each other and synergize, so that the plant growth regulator prepared finally can significantly improve the plant growth, increase the plant height and stem thickness, has a significant growth promotion effect, realizes the recycling use of resources, meets the green environmental protection requirement, and provides a new idea for sustainable and cyclic agriculture.

Description

Technical Field

[0001] This invention belongs to the field of plant growth regulators, specifically relating to a plant growth regulator containing camellia oleifera residue and its preparation method. Background Technology

[0002] Plant growth regulators are substances that regulate plant growth and development based on the structure, function, and mechanism of action of plant hormones. Inappropriate use of plant growth regulators during plant production can not only cause environmental pollution but also lead to phytotoxicity. Therefore, developing all-natural, green, and environmentally friendly plant growth regulators is crucial for both plant cultivation and environmental protection.

[0003] Camellia oleifera residue refers to the residue remaining after pressing oil from camellia oleifera fruits. Previously, this residue was often used directly as organic fertilizer in the soil without treatment, a crude and ineffective method. After application, the residue undergoes fermentation and decomposition under the action of microorganisms, generating a large amount of heat. If applied close to plant roots, this can cause root burn, affecting normal root growth and development, and in severe cases, even causing seedling death. Therefore, extra caution is required when applying it. Because camellia oleifera residue comes from natural camellia oleifera fruits, it is a pure, natural, and green raw material, environmentally friendly. Recent studies have found that camellia oleifera residue has various effects and benefits, and its application range is expanding. Therefore, developing safe and effective plant growth regulators using camellia oleifera residue as a raw material is an effective way to avoid environmental pollution and reduce the risk of pesticide damage. Summary of the Invention

[0004] To address the aforementioned problems, this invention provides a plant growth regulator containing camellia oleifera residue and its preparation method, which can effectively promote plant growth.

[0005] The technical solution of this invention is implemented as follows:

[0006] A plant growth regulator containing camellia oleifera residue, comprising the following components in parts by weight on a dry matter basis: 10-30 parts camellia oleifera residue, 5-15 parts shiitake mushroom meal, 3-10 parts distiller's grains, 1-3 parts Trichoderma harzianum, 2-4 parts yeast, 2-4 parts Bacillus amyloliquefaciens, 10-20 parts sugarcane bagasse, 20-30 parts banana stems and leaves, and 5-15 parts slaughterhouse waste.

[0007] Furthermore, the plant growth regulator comprises the following components in parts by weight: 20 parts camellia oleifera residue, 10 parts shiitake mushroom meal, 5 parts distiller's grains, 2 parts Trichoderma harzianum, 3 parts yeast, 3 parts Bacillus amyloliquefaciens, 15 parts sugarcane bagasse, 25 parts banana stems and leaves, and 10 parts slaughter waste.

[0008] Furthermore, the viable count of the *Trichoderma harzianum* is 10.7 -10 9 cfu / L, viable yeast count 10 7 -10 9 CFU / L, viable count of Bacillus amyloliquefaciens was 10. 7 -10 9 cfu / L.

[0009] Furthermore, the shiitake mushroom residue is the mushroom sticks or residue remaining after shiitake mushroom harvesting; the banana stems and leaves are the above-ground parts of the banana plant cut down after banana harvesting, which are then crushed and dried; the camellia oil residue is the residue remaining after pressing camellia oil fruit for oil; and the sugarcane residue is the residue remaining after sugarcane is pressed for sugar.

[0010] Furthermore, the slaughter waste refers to the bones, meat, and / or fur discarded during the slaughtering of livestock and poultry.

[0011] Furthermore, the livestock and poultry mentioned are chickens, ducks, geese, pigs, cattle, and / or sheep.

[0012] A method for preparing a plant growth regulator containing camellia oleifera residue includes the following steps:

[0013] (1) Take the prescribed amount of camellia residue, sugarcane residue and banana stems and leaves, and steam-explode them to obtain plant raw materials;

[0014] (2) Add shiitake mushroom residue and distiller's grains to the plant raw materials, mix well, adjust the moisture content to 30-40%, add Trichoderma harzianum and yeast, mix evenly, and pile for 15-20 days to obtain fermented material.

[0015] (3) Take the prescribed amount of slaughter waste, steam-explode it to obtain animal raw materials; after stirring, adjust the moisture content to 20-30%, add it to the fermentation material, and ferment for 1-3 days;

[0016] (4) Add Bacillus amyloliquefaciens to the above materials and continue fermentation for 5-10 days to obtain the final product.

[0017] Furthermore, the temperature should be controlled between 25-37℃ during fermentation. If the temperature exceeds 37℃, the pile should be turned and stirred to cool it down.

[0018] Furthermore, in step (1), the steam explosion treatment conditions are: maintaining pressure at a steam explosion pressure of 1.7-3.0 MPa for 70-200 seconds, followed by instantaneous release; in step (3), the steam explosion treatment conditions are: pressure between 2.5-3.5 MPa, pressure maintenance time of 3-6 minutes, followed by instantaneous release.

[0019] The above-mentioned plant growth regulators are used in the preparation of formulations that promote plant growth.

[0020] Furthermore, the plant growth regulator can increase plant height and / or stem diameter.

[0021] Furthermore, the plant in question is either camellia oleifera or banana.

[0022] Furthermore, in the aforementioned application, the dosage of the plant growth regulator is 100-300g / plant, applied once every 5-10 days, for a minimum of 3 applications.

[0023] The beneficial effects of this invention are:

[0024] The plant growth regulator of this invention uses agricultural waste such as camellia oleifera residue, shiitake mushroom meal, distiller's grains, sugarcane bagasse, banana stems and leaves, and waste generated during livestock and poultry slaughter as fermentation substrates. First, *Trichoderma harzianum* and yeast are added for fermentation, followed by the sequential addition of livestock and poultry slaughter waste and *Bacillus amyloliquefaciens* for further fermentation. Through this staged fermentation process, different microorganisms and substrates interact and work synergistically, ultimately producing a plant growth regulator that significantly enhances plant growth, increasing plant height and stem diameter, exhibiting a significant growth-promoting effect. This plant growth regulator, through a staged fermentation process, ensures thorough fermentation of all materials, avoiding problems such as seedling and root burn after application. It reduces the harm caused by directly using waste such as camellia oleifera residue, making it safe and convenient to use. It achieves the secondary utilization of agricultural waste such as camellia oleifera residue and shiitake mushroom meal, as well as livestock and poultry slaughter waste, avoiding environmental pollution caused by agricultural waste and livestock and poultry slaughter waste, and realizing the recycling of resources. The treatment of agricultural and slaughterhouse waste using steam explosion is not only highly efficient, but also requires no other chemical raw materials. The entire process is clean, safe, and meets the requirements of green environmental protection, providing a new approach for sustainable circular agriculture. Detailed Implementation

[0025] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0026] Example 1

[0027] A plant growth regulator containing camellia oil residue, comprising, by dry matter weight, the following components in parts by weight: 10 parts camellia oil residue, 5 parts shiitake mushroom spawn, 3 parts distiller's grains, 1 part *Trichoderma harzianum*, 2 parts yeast, 2 parts *Bacillus amyloliquefaciens*, 10 parts sugarcane bagasse, 20 parts banana stems and leaves, and 5 parts slaughterhouse waste. The *Trichoderma harzianum* has a viable count of 10. 7 cfu / L, viable yeast count 107 cfu / L.

[0028] (1) Cut fresh banana stems and leaves into 5-10cm sections and set aside; break up camellia dregs, shiitake mushroom residue, and distiller's grains separately and set aside; take the prescribed amount of camellia dregs, sugarcane residue, and banana stems and leaves cut into small sections, and steam-explode them to obtain plant raw materials; the steam-explosion treatment conditions are: maintain pressure at 1.7-3.0MPa for 70-200s, and then release instantaneously.

[0029] (2) Add shiitake mushroom meal and distiller's grains to the plant raw materials, mix well, crush, then adjust the moisture content to 30-40%, add Trichoderma harzianum and yeast, mix evenly, and pile for 15-20 days to obtain fermented material.

[0030] (3) Take the prescribed amount of slaughter waste, steam explosion treatment to obtain animal raw materials, adjust the moisture content to 20-30%, add it to the fermentation material, and ferment for 1-3 days; the steam explosion treatment conditions are: pressure between 2.5-3.5MPa, pressure maintenance time of 3-6 minutes, and then instantaneous release.

[0031] (4) Add Bacillus amyloliquefaciens to the above materials and continue fermentation for 5-10 days to obtain the final product.

[0032] In the above steps, the temperature is controlled between 25-37℃ during fermentation. If the temperature exceeds 37℃, the pile should be turned and stirred to cool it down.

[0033] Example 2

[0034] A plant growth regulator containing camellia oil residue, comprising, by dry matter weight, the following components in parts by weight: 20 parts camellia oil residue, 10 parts shiitake mushroom spawn, 5 parts distiller's grains, 2 parts *Trichoderma harzianum*, 3 parts yeast, 3 parts *Bacillus amyloliquefaciens*, 15 parts sugarcane bagasse, 25 parts banana stems and leaves, and 10 parts slaughterhouse waste. The *Trichoderma harzianum* has a viable count of 10-1. 8 cfu / L, viable yeast count 10 8 cfu / L.

[0035] (1) Cut fresh banana stems and leaves into 5-10cm sections and set aside; break up camellia residue and shiitake mushroom meal and set aside; take the prescribed amount of camellia residue, sugarcane residue and banana stems and leaves cut into small sections, and steam-explode them to obtain plant raw materials; the steam-explosion treatment conditions are: maintain pressure at 1.7-3.0MPa for 70-200s, and then release instantly.

[0036] (2) Add shiitake mushroom meal and distiller's grains to the plant raw materials, mix well, crush, then adjust the moisture content to 30-40%, add Trichoderma harzianum and yeast, mix evenly, and pile for 15-20 days to obtain fermented material.

[0037] (3) Take the prescribed amount of slaughter waste, steam explosion treatment to obtain animal raw materials, adjust the moisture content to 20-30%, add it to the fermentation material, and ferment for 1-3 days; the steam explosion treatment conditions are: pressure between 2.5-3.5MPa, pressure maintenance time of 3-6 minutes, and then instantaneous release.

[0038] (4) Add Bacillus amyloliquefaciens to the above materials and continue fermentation for 5-10 days to obtain the final product.

[0039] In the above steps, the temperature is controlled between 25-37℃ during fermentation. If the temperature exceeds 37℃, the pile should be turned and stirred to cool it down.

[0040] Example 3

[0041] A plant growth regulator containing camellia oil residue, comprising, by dry matter weight, the following components in parts by weight: 30 parts camellia oil residue, 15 parts shiitake mushroom spawn, 7 parts distiller's grains, 3 parts *Trichoderma harzianum*, 4 parts yeast, 4 parts *Bacillus amyloliquefaciens*, 20 parts sugarcane bagasse, 30 parts banana stems and leaves, and 15 parts slaughterhouse waste. The *Trichoderma harzianum* has a viable count of 10. 9 cfu / L, viable yeast count 10 9 cfu / L.

[0042] (1) Cut fresh banana stems and leaves into 5-10cm sections and set aside; break up camellia residue and shiitake mushroom meal and set aside; take the prescribed amount of camellia residue, sugarcane residue and banana stems and leaves cut into small sections, and steam-explode them to obtain plant raw materials; the steam-explosion treatment conditions are: maintain pressure at 1.7-3.0MPa for 70-200s, and then release instantly.

[0043] (2) Add shiitake mushroom meal and distiller's grains to the plant raw materials, mix well, crush, then adjust the moisture content to 30-40%, add Trichoderma harzianum and yeast, mix evenly, and pile for 15-20 days to obtain fermented material.

[0044] (3) Take the prescribed amount of slaughter waste, steam explosion treatment to obtain animal raw materials, adjust the moisture content to 20-30%, add it to the fermentation material, and ferment for 1-3 days; the steam explosion treatment conditions are: pressure between 2.5-3.5MPa, pressure maintenance time of 3-6 minutes, and then instantaneous release.

[0045] (4) Add Bacillus amyloliquefaciens to the above materials and continue fermentation for 5-10 days to obtain the final product.

[0046] In the above steps, the temperature is controlled between 25-37℃ during fermentation. If the temperature exceeds 37℃, the pile should be turned and stirred to cool it down.

[0047] In the above embodiments, the shiitake mushroom residue is the remaining mushroom logs or residue after shiitake mushroom harvesting. The banana stems and leaves are the above-ground parts remaining after banana harvesting. The camellia oil residue is the residue remaining after pressing camellia oil fruit for oil; the sugarcane bagasse is the residue remaining after sugarcane is pressed for sugar. Slaughter waste is at least one of the bones, meat, or hides discarded during the slaughter of livestock and poultry such as chickens, ducks, geese, pigs, cattle, or sheep. Distiller's grains are the residue remaining after brewing.

[0048] Trichoderma harzianum and Bacillus amyloliquefaciens were both purchased from Beihai Qiangxing Biotechnology Co., Ltd., and the yeast was commercially available Angel Yeast. Besides the above-mentioned inoculants, inoculants produced by other companies can also be used; this invention does not impose any particular limitation.

[0049] The plant growth regulators described in the above embodiments can promote plant growth, increase plant height and stem diameter, and can be used as base fertilizer or top dressing. The dosage is 100-300g / plant, applied once every 5-10 days, for at least 3 applications.

[0050] Effect study of the plant growth regulator described in this invention

[0051] Different plant growth regulators were prepared according to the methods described in Table 1 for the experiment. Adjacent but not contiguous plots were selected, and surviving Camellia oleifera seedlings and banana seedlings from the same batch were used as experimental subjects, with 30 seedlings in each treatment. The growth-promoting effect of the plant growth regulators described in this invention was investigated. Each Camellia oleifera seedling was treated with 300g, and each banana seedling with 100g. During the experiment, a trench was dug near the base of the seedling stem, taking care to avoid damaging the seedling roots. The prepared growth regulator was applied, and then covered with soil. Applications were made every 10 days. The blank control group received no treatment. All treatment groups underwent the same routine management. Starting from the first application, the plant height of each treatment group was measured with a tape measure before treatment and on day 60 after treatment. The stem diameter of the Camellia oleifera and banana seedlings was measured close to the ground using calipers. The increases in plant height and stem diameter were calculated, and the average of the results was taken.

[0052] Table 1. Composition, preparation method, and application method of growth regulators for each treatment.

[0053]

[0054] Table 2. Effects of each treatment on the growth of Camellia oleifera seedlings

[0055]

[0056] Table 3 Effects of each treatment on banana seedling growth

[0057]

[0058] As can be seen from Tables 2 and 3, the plant height and stem diameter of Camellia oleifera seedlings and banana seedlings were significantly increased when the growth regulators prepared in Examples 1-3 of this invention were applied to the treatment groups. The increase in plant height and stem diameter was not only significantly higher than that of treatment group 11 without any treatment, but also significantly higher than that of treatment groups 4-7 with the changed composition of the growth regulators. This indicates that the growth regulators prepared in the three examples of this invention have a significantly enhanced effect compared with treatment groups 4-7 and the blank control group.

[0059] Treatment groups 8-9 used the same composition and preparation method as in Example 2, but due to the different number of applications, the increases in plant height and stem diameter varied significantly, indicating a marked difference in growth-promoting effect. In treatment groups 8 and 9, which were applied once and twice respectively, the increases in plant height and stem diameter of the camellia and banana seedlings were significantly reduced. Therefore, using the plant growth regulator described in this invention, applying it more than three times significantly enhances the growth-promoting effect. In treatment group 10, the simultaneous addition of the three microbial agents during fermentation also significantly reduced their effect, indicating that the separate addition of the microbial agents in two applications significantly promotes the growth-promoting effect.

[0060] No seedlings in any of the treatment groups experienced problems such as seedling burn or root burn, indicating that the growth regulators obtained through fermentation in each treatment group will not affect the normal growth of the plants, greatly improving safety, making them convenient to apply, and suitable for large-scale application in the field.

[0061] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A plant growth regulator containing oil tea residue, characterized by, Camellia oleifera residue 10-30 parts, Lentinula edodes residue 5-15 parts, distiller's grains 3-10 parts, Trichoderma harzianum 1-3 parts, yeast 2-4 parts, Bacillus amyloliquefaciens 2-4 parts, sugarcane residue 10-20 parts, banana stem and leaf 20-30 parts, and slaughter waste 5-15 parts by weight based on the weight of dry matter; A preparation method thereof comprises the following steps: (1) taking formula amount of Camellia oleifera residue, sugarcane residue and banana stem and leaf, and obtaining plant raw materials after steam explosion treatment; (2) adding Lentinula edodes residue and distiller's grains to the plant raw materials, mixing, adjusting the moisture content to 30-40%, adding Trichoderma harzianum and yeast, mixing uniformly, and stacking for 15-20 days to obtain fermented materials; (3) taking formula amount of slaughter waste, and obtaining animal raw materials after steam explosion treatment; adjusting the moisture content to 20-30% after stirring, and adding to the fermented materials for fermentation for 1-3 days; (4) adding Bacillus amyloliquefaciens to the above materials for further fermentation for 5-10 days.

2. The plant growth regulator containing oil tea residue according to claim 1, characterized in that, The plant growth regulator is composed of the following components in parts by weight: Camellia oleifera residue 20 parts, Lentinula edodes residue 10 parts, distiller's grains 5 parts, Trichoderma harzianum 2 parts, yeast 3 parts, Bacillus amyloliquefaciens 3 parts, sugarcane residue 15 parts, banana stem and leaf 25 parts, and slaughter waste 10 parts.

3. The plant growth regulator containing oil tea residue according to claim 1, characterized in that, The viable cell number of the Trichoderma harzianum is 10 7 -10 9 cfu / L, the viable cell number of the yeast is 10 7 -10 9 cfu / L, the viable cell number of the Bacillus amyloliquefaciens is 10 7 -10 9 cfu / L.

4. The plant growth regulator containing oil tea residue according to claim 1, characterized in that, The Lentinula edodes residue is the residual stick or residue after Lentinula edodes is harvested; the banana stem and leaf is the aboveground part of banana plant after banana is harvested, which is crushed and dried; the Camellia oleifera residue is the residual stick after Camellia oleifera fruit is pressed for oil; the sugarcane residue is the residual stick after sugarcane is pressed for sugar; and the slaughter waste is the bone, meat and / or fur discarded in the process of slaughtering livestock and poultry.

5. The plant growth regulator containing oil tea residue according to claim 1, wherein the oil tea residue is obtained by extracting oil from the seeds of Camellia oleifera Abel. and then drying the extracted seeds. The temperature is controlled to be 25-37 ℃ during fermentation, and the temperature is reduced by turning and stirring when the temperature exceeds 37 ℃.

6. The plant growth regulator containing oil tea residue according to claim 1, characterized in that, In the step (1), the steam explosion treatment condition is that the pressure is maintained at 1.7-3.0 MPa for 70-200 s, and then instantaneously released; and in the step (3), the steam explosion treatment condition is that the pressure is maintained at 2.5-3.5 MPa for 3-6 min, and then instantaneously released.

7. Use of the plant growth regulator according to any one of claims 1-6 in the preparation of a preparation for promoting plant growth.

8. Use according to claim 7, wherein the compound is ###0002### The plant growth regulator can increase the plant height and / or stem diameter of plants; and the plants are Camellia oleifera or banana.

9. The use according to claim 7, wherein the compound is ###00003### 8 or ###00004### 9. In the use, the plant growth regulator is used in an amount of 100-300 g / plant, and is applied once every 5-10 days, at least 3 times.

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