Efficient germination cultivation method for juicy peach seeds based on improved culture medium

Abstract

The invention provides a high-efficiency juicy peach seed germination cultivation method based on an improved culture medium, which comprises the following steps: step 1, seed collection and pretreatment: seeds are subjected to low-temperature stratification treatment; step 2, preparation of an improved culture medium: based on the MS culture medium, adding the following components: 6-benzyladenine (6-BA), indolebutyric acid (IBA), gibberellin (GA), activated carbon, cane sugar and agar; step 3, seed germination and culture: inoculating the disinfected seeds to an improved culture medium under a sterile condition, keeping the culture medium moist, and promoting radicle breakthrough; step 4, seedling domestication and transplanting: when 2-3 true leaves grow on the seedlings, the seedlings are transferred to nutrient soil for domestication, illumination and humidity are adjusted step by step, and the seedlings are transplanted to a field after domestication. By improving the optimal proportion of the culture medium, the germination rate of the juicy peach seeds is remarkably increased. When the culture medium is improved, gibberellin (GA), 6-BA and other plant hormones are utilized, the dormancy period can be effectively shortened, the seed germination process is accelerated, and the seedling raising period is shortened.

Description

Technical Field

[0001] The present invention relates to the technical field of peach cultivation, specifically to an efficient germination cultivation method for peach seeds based on a modified culture medium. Background Technique

[0002] Peach (Prunus persica) is very popular in the market because of its tender flesh, abundant juice and delicious taste. However, due to the long dormancy period and low natural germination rate of peach seeds, there are problems such as long cycle, low germination rate and unstable seedling quality in the process of seed propagation. Existing seed treatment methods, such as cold stratification, chemical treatment, etc., can improve the germination rate to a certain extent, but there are still deficiencies such as long cycle or the risk of using chemical reagents. Therefore, there is an urgent need for an improved method that can improve the germination rate of peach seeds, shorten the seedling raising cycle and ensure the robust growth of seedlings.

[0003] Therefore, we propose an efficient germination cultivation method for peach seeds based on a modified culture medium. Summary of the Invention

[0004] The purpose of the present invention is to provide an efficient germination cultivation method for peach seeds based on a modified culture medium, using MS (Murashige and Skoog) medium to promote the germination of peach seeds, and cultivating them into complete plants through transplanting and acclimatizing seedlings, so as to improve the germination rate and seedling quality of peach seeds, shorten the seedling raising cycle and increase its economic benefits.

[0005] To achieve the above purpose, the present invention provides the following technical solutions: An efficient germination cultivation method for peach seeds based on a modified culture medium, comprising the following steps: Step 1. Seed collection and pretreatment, placing the seeds in low-temperature stratification treatment; Step 2. Preparation of the modified culture medium, based on MS medium, adding the following components: 6-benzyladenine (6-BA), indolebutyric acid (IBA), gibberellin (GA3), activated carbon, sucrose, agar; Step 3. Seed germination and cultivation, inoculating the disinfected seeds into the modified culture medium under sterile conditions, keeping the culture medium moist, and promoting the radicle to break through; Step 4. Seedling acclimatization and transplantation, when the seedlings grow 2-3 true leaves, transfer them to nutrient soil for acclimatization, gradually adjust the light and humidity, and transplant them to the field after acclimatization.

[0006] Preferably, in the first step, the seeds selected are the seeds of mature and disease-free fruits, and the residual pulp is washed and removed.

[0007] Preferably, in the first step, the seeds are placed in a 4°C environment for low-temperature stratification treatment for 7-10 days to break dormancy.

[0008] Preferably, the added components of the improved culture medium in step two are specifically: 6-Benzyladenine (6-BA) 1.0 mg / L, Indolebutyric acid (IBA) 0.5 mg / L, Gibberellin (GA3) 1.0 mg / L, Activated carbon 0.5 g / L, Sucrose 30 g / L, Agar 8 g / L.

[0009] Preferably, adjust the pH of the improved culture medium to 5.7 - 6.0 and autoclave at 121 °C for 20 minutes.

[0010] Preferably, in step three, inoculate the seeds into the improved culture medium under sterile conditions and culture for 7 - 14 days, and control the culture conditions as: light 16 hours / day, temperature 25 ± 2 °C, humidity 70 - 80%.

[0011] Preferably, the composition components of the nutrient soil in step four are: Peat soil : Perlite : Vermiculite = 2 : 1 : 1.

[0012] Preferably, spray 0.1 mg / L IBA solution during the seedling acclimation stage, gradually increase the light to natural light intensity, and reduce the humidity to the natural environment level 2 - 3 weeks before transplantation to complete the acclimation.

[0013] Compared with the prior art, the beneficial effects of the present invention are: Through the optimized ratio of the improved culture medium, the present invention significantly improves the germination rate of peach seeds. The improved culture medium utilizes plant hormones such as gibberellin (GA3) and 6-BA, which can effectively shorten the dormancy period and accelerate the seed germination process, and shorten the seedling raising cycle.

[0014] The appropriate amount of activated carbon in the improved culture medium can promote root growth, improve the stress resistance of seedlings, and at the same time, the acclimation process optimizes the adaptability of seedlings and improves the transplanting survival rate.

[0015] This application adopts physical and biological regulation means, without using strong irritating chemical agents, to ensure the safety and sustainability of seedling raising. Specific embodiments

[0016] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0017] A method for highly efficient germination cultivation of peach seeds based on an improved culture medium, characterized by comprising the following steps: Step 1: Seed collection and pretreatment Select ripe honey peach fruits without pests and diseases, remove the seeds and wash them clean; Remove the remaining peel and pulp, and rinse the surface with clean water; Place the seeds in a low-temperature stratification treatment at 4°C for 7 - 10 days to break seed dormancy and improve germination vigor.

[0018] Step 2: Preparation of improved medium Use MS (Murashige and Skoog) medium as the basis and add the following improved components: 6-Benzyladenine (6-BA) 1.0 mg / L Indolebutyric acid (IBA) 0.5 mg / L Gibberellin (GA3) 1.0 mg / L Activated carbon 0.5 g / L Sucrose 30 g / L Agar 8 g / L; Then adjust the pH value of the medium to 5.7 - 6.0, sterilize it at 121°C for 20 minutes, and set it aside after cooling.

[0019] Step 3: Seed germination and culture Use 4% sodium hypochlorite (NaOCl) solution to disinfect the seed surface, soak for 5 - 10 minutes and then rinse with sterile water.

[0020] Under aseptic operation environment, inoculate the disinfected honey peach seeds onto the improved medium.

[0021] Cultivate in a culture room with 16 hours of light per day and a temperature of 25 ± 2°C.

[0022] In the initial culture stage (7 - 14 days), appropriately adjust the moisture content of the medium to keep the surface of the medium moist but not waterlogged, so as to promote the softening of the seed coat and accelerate the breakthrough of the radicle.

[0023] Observe the seed germination situation, remove the ungerminated or mildewed seeds, and ensure that the culture environment is clean and pollution-free.

[0024] Step 4: Seedling cultivation and transplantation When the seedlings grow to 2 - 3 true leaves, transfer them to nutrient soil (peat soil, perlite and vermiculite mixed in a ratio of 2:1:1) for acclimation culture; Spray a low-concentration growth regulator 0.1 mg / L IBA to promote root development; Gradually increase the light intensity to natural light and maintain an appropriate humidity (70 - 80%) to improve the survival rate of the seedlings; After 2–3 weeks, gradually reduce the humidity and minimize manual intervention to acclimatize the seedlings to the external environment, and finally transplant them to the field or orchard.

[0025] During the nectarine experiment, by adjusting the ratios of 6-benzyladenine (6-BA), indolebutyric acid (IBA), and gibberellin (GA3), different modified media were prepared for testing. 30 seeds were used in each group of experiments, and the experiments were repeated 3 times, as follows: Example 1 Step 1: Select nectarine fruits with appropriate maturity and no pests or diseases. Remove the seeds, wash them thoroughly, and place them in a 4°C environment for low-temperature stratification for 10 days. Step 2: Use MS (Murashige and Skoog) medium as the base and add the following modified components: 6-benzyladenine (6-BA) 0.5 mg / L, indolebutyric acid (IBA) 0.25 mg / L, gibberellin (GA3) 0.5 mg / L, activated carbon 0.5 g / L, sucrose 30 g / L, agar 8 g / L; adjust the pH value to 6.0, autoclave at 121°C for 20 minutes, and set aside after cooling. Step 3: Disinfect the seed surface with 4% sodium hypochlorite (NaOCl) solution, soak for 8 minutes, and then rinse with sterile water; under a sterile operation environment, inoculate the disinfected nectarine seeds onto the modified medium and culture them in an incubator with 12 hours of light per day, 60% humidity, and a temperature of 25°C for 10 days.

[0026] Step 4: When the seedlings grow to 2–3 true leaves, transfer them to nutrient soil (a mixture of peat soil, perlite, and vermiculite in a ratio of 2:1:1) for acclimation culture; gradually increase the light intensity to natural light and maintain a humidity of 70%. After 2 weeks, gradually reduce the humidity and minimize manual intervention to acclimatize the seedlings to the external environment, and finally transplant them to the field or orchard.

[0027] Example 2 Step 1: Select nectarine fruits with appropriate maturity and no pests or diseases. Remove the seeds, wash them thoroughly, and place them in a 4°C environment for low-temperature stratification for 10 days. Step 2: Use MS (Murashige and Skoog) medium as the base and add the following modified components: 6-benzyladenine (6-BA) 0.5 mg / L, indolebutyric acid (IBA) 0.25 mg / L, gibberellin (GA3) 0.5 mg / L, activated carbon 0.5 g / L, sucrose 30 g / L, agar 8 g / L; adjust the pH value to 6.0, autoclave at 121°C for 20 minutes, and set aside after cooling. Step 3: Disinfect the seed surface with 4% sodium hypochlorite (NaOCl) solution, rinse with sterile water after soaking for 8 minutes; under a sterile operation environment, inoculate the disinfected peach seeds onto the modified medium, and culture them in an incubator with 16 hours of light per day, 70% humidity, and a temperature of 25°C for 10 days.

[0028] Step 4: When the seedlings grow to 2 - 3 true leaves, transfer them to nutrient soil (peat soil, perlite, and vermiculite mixed at a ratio of 2:1:1) and spray a low - concentration growth regulator, 0.1 mg / L IBA, for acclimation culture. Gradually increase the light intensity to natural light and maintain 70% humidity; after 2 weeks, gradually reduce the humidity and reduce artificial intervention to enable the seedlings to adapt to the external environment, and finally transplant them to the field or orchard.

[0029] Example 3 Step 1: Select ripe and disease - and - pest - free peach fruits, take out the seeds, wash them clean, and place them in a 4°C environment for low - temperature stratification treatment for 10 days; Step 2: Use MS (Murashige and Skoog) medium as the basis and add the following modified components: 6 - benzyladenine (6 - BA) 0.5 mg / L, indole - 3 - butyric acid (IBA) 0.25 mg / L, gibberellin (GA3) 0.5 mg / L, activated carbon 0.5 g / L, sucrose 30 g / L, agar 8 g / L; adjust the pH value to 6.0, autoclave at 121°C for 20 minutes, and set aside after cooling; Step 3: Disinfect the seed surface with 4% sodium hypochlorite (NaOCl) solution, rinse with sterile water after soaking for 8 minutes; under a sterile operation environment, inoculate the disinfected peach seeds onto the modified medium, and culture them in an incubator with 16 hours of light per day, 80% humidity, and a temperature of 25°C for 10 days.

[0030] Step 4: When the seedlings grow to 2 - 3 true leaves, transfer them to nutrient soil (peat soil, perlite, and vermiculite mixed at a ratio of 2:1:1) and spray a low - concentration growth regulator, 0.2 mg / L IBA, for acclimation culture. Gradually increase the light intensity to natural light and maintain 70% humidity; after 2 weeks, gradually reduce the humidity and reduce artificial intervention to enable the seedlings to adapt to the external environment, and finally transplant them to the field or orchard.

[0031] Example 4 Step 1: Select ripe and disease - and - pest - free peach fruits, take out the seeds, wash them clean, and place them in a 4°C environment for low - temperature stratification treatment for 10 days; Step 2: Use MS (Murashige and Skoog) medium as the basis and add the following modified components: 6-Benzyladenine (6-BA) 1.0 mg / L, indolebutyric acid (IBA) 0.5 mg / L, gibberellin (GA3) 1 mg / L, activated carbon 0.5 g / L, sucrose 30 g / L, agar 8 g / L; adjust the pH value to 6.0, autoclave at 121 °C for 20 minutes, and set aside after cooling; Step 3. Disinfect the seed surface with 4% sodium hypochlorite (NaOCl) solution, rinse with sterile water after soaking for 8 minutes; under a sterile operation environment, inoculate the disinfected peach seeds onto the modified medium, and culture them in an incubator at a light intensity of 12 hours per day, humidity of 60%, and temperature of 25 °C for 10 days.

[0032] Step 4. When the seedlings grow to 2-3 true leaves, transfer them to nutrient soil (peat soil, perlite, and vermiculite mixed at a ratio of 2:1:1) and spray a low-concentration growth regulator of 0.1 mg / L IBA for acclimation culture. Gradually increase the light intensity to natural light and maintain a humidity of 70%; after 2 weeks, gradually reduce the humidity and reduce artificial intervention to enable the seedlings to adapt to the external environment, and finally transplant them to the field or orchard.

[0033] Example 5 Step 1. Select peach fruits with appropriate maturity and no pests or diseases, remove the seeds, wash them clean, and place them in a 4 °C environment for low-temperature stratification for 10 days; Step 2. Use MS (Murashige and Skoog) medium as the basis and add the following modified components: 6-Benzyladenine (6-BA) 1.0 mg / L, indolebutyric acid (IBA) 0.5 mg / L, gibberellin (GA3) 1 mg / L, activated carbon 0.5 g / L, sucrose 30 g / L, agar 8 g / L; adjust the pH value to 6.0, autoclave at 121 °C for 20 minutes, and set aside after cooling; Step 3. Disinfect the seed surface with 4% sodium hypochlorite (NaOCl) solution, rinse with sterile water after soaking for 8 minutes; under a sterile operation environment, inoculate the disinfected peach seeds onto the modified medium, and culture them in an incubator at a light intensity of 16 hours per day, humidity of 70%, and temperature of 25 °C for 10 days.

[0034] Step 4. When the seedlings grow to 2-3 true leaves, transfer them to nutrient soil (peat soil, perlite, and vermiculite mixed at a ratio of 2:1:1) for acclimation culture. Gradually increase the light intensity to natural light and maintain a humidity of 70%; after 2 weeks, gradually reduce the humidity and reduce artificial intervention to enable the seedlings to adapt to the external environment, and finally transplant them to the field or orchard.

[0035] Example 6 Step 1: Select ripe and suitable honey peach fruits without pests and diseases. Take out the seeds, wash them clean, and place them in a 4°C environment for low-temperature stratification treatment for 10 days. Step 2: Use MS (Murashige and Skoog) medium as the basis and add the following modified components: 6-benzyladenine (6-BA) 1.5 mg / L, indole-3-butyric acid (IBA) 0.75 mg / L, gibberellin (GA3) 1.5 mg / L, activated carbon 0.5 g / L, sucrose 30 g / L, agar 8 g / L; adjust the pH value to 6.0, autoclave at 121°C for 20 minutes, and set aside after cooling. Step 3: Disinfect the seed surface with 4% sodium hypochlorite (NaOCl) solution, soak for 8 minutes, and then rinse with sterile water; under a sterile operation environment, inoculate the disinfected honey peach seeds onto the modified medium, and culture them in an incubator with 12 hours of light per day, 60% humidity, and a temperature of 25°C for 10 days.

[0036] Step 4: When the seedlings grow to 2 - 3 true leaves, transfer them to nutrient soil (peat soil, perlite, and vermiculite mixed at a ratio of 2:1:1) and spray a low-concentration growth regulator 0.2 mg / L IBA for acclimation culture. Gradually increase the light intensity to natural light and maintain a humidity of 70%; after 2 weeks, gradually reduce the humidity and reduce artificial intervention to enable the seedlings to adapt to the external environment, and finally transplant them to the field or orchard.

[0037] Example 7 Step 1: Select ripe and suitable honey peach fruits without pests and diseases. Take out the seeds, wash them clean, and place them in a 4°C environment for low-temperature stratification treatment for 10 days. Step 2: Use MS (Murashige and Skoog) medium as the basis and add the following modified components: 6-benzyladenine (6-BA) 1.5 mg / L, indole-3-butyric acid (IBA) 0.75 mg / L, gibberellin (GA3) 1.5 mg / L, activated carbon 0.5 g / L, sucrose 30 g / L, agar 8 g / L; adjust the pH value to 6.0, autoclave at 121°C for 20 minutes, and set aside after cooling. Step 3: Disinfect the seed surface with 4% sodium hypochlorite (NaOCl) solution, soak for 8 minutes, and then rinse with sterile water; under a sterile operation environment, inoculate the disinfected honey peach seeds onto the modified medium, and culture them in an incubator with 16 hours of light per day, 80% humidity, and a temperature of 25°C for 10 days.

[0038] Step 4: When the seedlings grow to 2 - 3 true leaves, transfer them to nutrient soil (peat soil, perlite, and vermiculite mixed at a ratio of 2:1:1) for acclimation cultivation. Gradually increase the light intensity to natural light and maintain a humidity of 70%. After 2 weeks, gradually reduce the humidity and minimize artificial intervention to enable the seedlings to adapt to the external environment, and finally transplant them to the field or orchard.

[0039] Example 8 Step 1: Select ripe and pest - and - disease - free peach fruits, take out the seeds, wash them clean, and place them in a 4°C environment for low - temperature stratification treatment for 10 days. Step 2: Use MS (Murashige and Skoog) medium as the base and add the following modified components: 6 - benzyladenine (6 - BA) 1.0 mg / L, indole - 3 - butyric acid (IBA) 0.5 mg / L, gibberellin (GA3) 1.0 mg / L, activated carbon 0.5 g / L, sucrose 30 g / L, agar 8 g / L; adjust the pH value to 6.0, autoclave at 121°C for 20 minutes, and set aside after cooling. Step 3: Disinfect the seed surface with 4% sodium hypochlorite (NaOCl) solution, soak for 8 minutes, and then rinse with sterile water. Under a sterile operation environment, inoculate the disinfected peach seeds onto the modified medium and culture them in an incubator at a light intensity of 16 hours per day, a humidity of 70%, and a temperature of 25°C for 10 days.

[0040] Step 4: When the seedlings grow to 2 - 3 true leaves, transfer them to nutrient soil (peat soil, perlite, and vermiculite mixed at a ratio of 2:1:1) and spray a low - concentration growth regulator 0.1 mg / L IBA for acclimation cultivation. Gradually increase the light intensity to natural light and maintain a humidity of 70%. After 2 weeks, gradually reduce the humidity and minimize artificial intervention to enable the seedlings to adapt to the external environment, and finally transplant them to the field or orchard.

[0041] Calculate key indicators such as germination rate, germination uniformity, survival rate, and root index for the experimental results of Examples 1 - 8 respectively, conduct a systematic analysis, and form the following comprehensive statistical table 1 as follows:

[0042] Table 1 Among them, the data are the average value ± standard deviation of 3 repeated experiments.

[0043] In this application, spraying a low - concentration growth regulator IBA for acclimation cultivation can improve the survival rate and increase the root biomass, making IBA essential for transplantation.

[0044] In this application, when the humidity of the improved culture medium is 80%, the germination rate is lower than that when the humidity is 70%, and mildew is observed with the naked eye. Therefore, it is necessary to control the humidity ≤ 70%.

[0045] Meanwhile, the extension of light also promotes the germination rate.

[0046] Based on the above analysis, Example 8 of this application is the optimal solution with relatively high comprehensive performance, providing reliable technical parameters for the industrialized seedling cultivation of peaches. In addition, this application also compares with the traditional planting method and the conventional tissue culture method as follows: Comparative Example 1 Adopt the traditional planting method Select peaches with appropriate maturity and no diseases and pests, take out the seeds, wash them clean, and directly sow them after natural drying.

[0047] Comparative Example 2 Adopt the conventional tissue culture method Select peaches with appropriate maturity and no diseases and pests, take out the seeds, wash them clean, soak them in alcohol for disinfection, and then dry and sow them.

[0048] The differences between this application and the traditional planting method and the conventional tissue culture method are shown in Table 2 below: Comparison Dimension Example 8 Comparative Example 1: Traditional Planting Method Comparative Example 2: Conventional Tissue Culture Method Seed Pretreatment Stratification at 4°C for 7 - 10 days + Sodium Hypochlorite Disinfection Sow directly after natural air-drying Disinfection by alcohol immersion Germination Rate (%) 96.4±1.1 70 - 80 (greatly affected by season) 85±5 Germination Period (days) 14 - 21 days (radicle breakthrough) 30 - 45 days 25 - 35 days Medium Cost (yuan / liter) 28.5 (including hormones + activated carbon) 0 (soil substrate) 65.0 (including expensive medium components) Survival Rate of Seedlings (%) 97.3 ± 0.9 (after transplantation) 60-70 88±4 Total Cultivation Period (days) 60 - 75 days (including acclimation period) 90 - 120 days 80 - 100 days Table 2 Compared with the traditional planting method and the conventional tissue culture method, this application significantly improves the germination rate of peach seeds by optimizing the proportion of the improved culture medium, with a 20–30% increase compared to the traditional method. When improving the culture medium, plant hormones such as gibberellin (GA3) and 6-BA are used, which can effectively shorten the dormancy period, accelerate the seed germination process, and shorten the seedling cultivation cycle.

[0049] An appropriate amount of activated carbon in the improved culture medium can promote root growth, improve the stress resistance of seedlings. Meanwhile, the acclimation process optimizes the adaptability of seedlings and improves the transplanting survival rate.

[0050] This application adopts physical and biological regulation means, without using strong irritating chemical agents, ensuring the safety and sustainability of seedling cultivation.

[0051] It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. And the term "comprising", "including" or any other variant thereof is intended to cover a non-exclusive inclusion, so that a process, method, article or device comprising a series of elements not only includes those elements, but also includes other elements not expressly listed, or elements inherent to such process, method, article or device.

[0052] Although embodiments of the present invention have been shown and described, those of ordinary skill in the art will understand that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for efficiently germinating and cultivating peach seeds based on an improved culture medium, characterized in that, It includes the following steps: Step 1: Seed collection and pretreatment. Place the seeds in low-temperature stratification treatment. Step 2: Preparation of the improved culture medium. Based on the MS medium, add the following components: 6-benzyladenine (6-BA), indolebutyric acid (IBA), gibberellin (GA3), activated carbon, sucrose, and agar. Step 3: Seed germination and cultivation. Under sterile conditions, inoculate the disinfected seeds into the improved culture medium, keep the culture medium moist, and promote the radicle to break through. Step 4: Seedling acclimation and transplantation. When the seedlings have 2-3 true leaves, transfer them to nutrient soil for acclimation, gradually adjust the light and humidity, and transplant them to the field after acclimation.

2. The method for highly efficient germination cultivation of peach seeds based on an improved culture medium according to claim 1, characterized in that: In Step 1, the seeds selected are the seeds of mature and disease-free fruits, which are washed and the residual pulp is removed.

3. The method for efficiently germinating and cultivating peach seeds based on an improved culture medium according to claim 1, wherein: In Step 1, the seeds are placed in a 4°C environment for low-temperature stratification treatment for 7-10 days to break dormancy.

4. The method for highly efficient germination cultivation of peach seeds based on the improved culture medium according to claim 1, characterized in that: The specific added components of the improved culture medium in Step 2 are as follows: 6-benzyladenine (6-BA) 1.0 mg / L, indolebutyric acid (IBA) 0.5 mg / L, gibberellin (GA3) 1.0 mg / L, activated carbon 0.5 g / L, sucrose 30 g / L, and agar 8 g / L.

5. The method for efficiently germinating and cultivating peach seeds based on the improved culture medium according to claim 4, characterized in that: Adjust the pH of the improved culture medium to 5.7-6.0 and autoclave it at 121°C for 20 minutes.

6. The method for highly efficient germination cultivation of peach seeds based on the improved culture medium according to claim 5, wherein: In Step 3, inoculate the seeds into the improved culture medium under sterile conditions and culture for 7-14 days. Control the culture conditions as follows: light 16 hours per day, temperature 25±2°C, and humidity 70-80%.

7. The method for efficiently germinating and cultivating peach seeds based on an improved culture medium according to claim 6, characterized in that: The composition of the nutrient soil in Step 4 is: peat soil: perlite: vermiculite = 2:1:

1.

8. The method for highly efficient germination cultivation of honey peach seeds based on the improved culture medium according to claim 7, characterized in that: During the seedling acclimation stage, spray a 0.1 mg / L IBA solution, gradually increase the light to the natural light intensity, and reduce the humidity to the natural environment level 2-3 weeks before transplantation to complete the acclimation.

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