Radix polygoni multiflori life-prolonging pill compound composition, preparation method thereof and hair care solution

Abstract

The invention relates to the field of cosmetics, in particular to a fleece-flower root life-prolonging pill compound composition, application of the fleece-flower root life-prolonging pill compound composition and a preparation method of a hair care solution. According to the fleece-flower root life-prolonging pill compound composition provided by the invention, on the basis of a core formula of a traditional fleece-flower root life-prolonging pill, the cacumen biotae and the tanshinone are introduced, and the cacumen biotae and the tanshinone can exert a triple synergistic mechanism of oxidation resistance, microcirculation promotion and hair follicle cycle regulation in the core formula of the fleece-flower root life-prolonging pill; specifically, the inhibition rate of 5 alpha reductase is increased synergistically, so that high oil control, anti-inflammatory and hair follicle dredging effects can be achieved, and the skin permeability is good. The invention successfully develops an efficient and safe traditional Chinese medicine hair care product with an anti-hair-loss synergistic effect by fusing a modern extraction technology, a scientific compounding system and a stable preparation process and by virtue of a traditional fleece-flower root life-prolonging pill compound, and provides a new solution for solving the technical problems of the existing traditional Chinese medicine hair care product.

Description

Technical Field

[0001] The present invention relates to the field of cosmetics, and specifically to a compound composition of Shouwu Yanshou Dan, its application, a hair care liquid, and a preparation method of the hair care liquid. Background Art

[0002] With the acceleration of the pace of modern social life and the aggravation of environmental pollution, problems such as hair loss, white hair, and hair aging are becoming increasingly serious and showing a trend of younger age. Most of the current hair care products on the market are mainly composed of chemical components, such as sulfate surfactants, silicone oil, etc. Although these components can provide short-term softening effects, long-term use may damage the scalp barrier and even cause hair follicle atrophy. In the field of anti-hair loss and anti-aging, the existing technologies mainly rely on western medicine components, such as minoxidil and finasteride, but these drugs often come with side effects such as scalp irritation and hormone disorders, bringing health risks to consumers.

[0003] Traditional Chinese medicine theory believes that "hair is the surplus of blood", emphasizing that the health of hair is closely related to the sufficiency of qi and blood. As a classic representative of the theory of "simultaneously regulating the liver and kidneys" in traditional Chinese medicine, Wu Yanshou Dan has always been used in the treatment of premature graying of hair and beard in the form of oral pills since it was recorded in "Medical Books of the Shibu Studio" in the Qing Dynasty.

[0004] However, there are three major bottlenecks in the application of traditional Shouwu Yanshou Dan pills: limited bioavailability, poor dosage form adaptability, and insufficient scientific compatibility. Specifically, one of the main components in traditional Shouwu Yanshou Dan pills is Polygonum multiflorum Thunb., and the bioavailability of its key active ingredient stilbene glycoside is only about 8% after the first-pass effect of the gastrointestinal tract, and it needs to act on hair follicles indirectly through the metabolism of the liver and kidneys, and the dose reaching the scalp hair follicles is less than 0.5% of the intake. In addition, the poor dosage form adaptability and insufficient scientific compatibility of traditional Shouwu Yanshou Dan pills also make it impossible to achieve better oil control, anti-inflammatory, and hair follicle dredging effects when formulated into a hair care liquid. Summary of the Invention

[0005] In view of this, the technical problem to be solved by the present invention is to provide a compound composition of Shouwu Yanshou Dan, its application, a hair care liquid, and a preparation method of the hair care liquid. The Shouwu Yanshou Dan composition provided by the present invention has high oil control, anti-inflammatory, and hair follicle dredging effects and good transdermal permeability.

[0006] A compound composition of Shouwu Yanshou Dan, calculated by weight, includes 0.1 - 1 part of tanshinone and 1 - 50 parts of a compound medicinal material extract, and the compound medicinal material extract is made from the following components:

[0007] Polygonum multiflorum Thunb. and / or Polygonum multiflorum Thunb. extract: 10 - 40 parts;

[0008] Siegesbeckia orientalis L. and / or Siegesbeckia orientalis L. extract: 1 - 10 parts;

[0009] Cuscuta chinensis and / or Cuscuta chinensis extract: 1 - 10 parts;

[0010] Mulberry and / or mulberry extract: 1 - 10 parts;

[0011] Black sesame and / or black sesame extract: 1 - 10 parts;

[0012] Rosae Laevigatae Fructus and / or Rosae Laevigatae Fructus extract: 1 - 10 parts;

[0013] Ecliptae Herba and / or Ecliptae Herba extract: 1 - 10 parts;

[0014] Platycladi Cacumen and / or Platycladi Cacumen extract: 0.5 - 8 parts;

[0015] Eucommiae Cortex and / or Eucommiae Cortex extract: 0.5 - 5 parts;

[0016] Achyranthis Bidentatae Radix and / or Achyranthis Bidentatae Radix extract: 0.5 - 5 parts;

[0017] Ligustri Lucidi Fructus and / or Ligustri Lucidi Fructus extract: 0.5 - 5 parts;

[0018] Mori Folium and / or Mori Folium extract: 0.1 - 3 parts;

[0019] Lonicerae Japonicae Caulis and / or Lonicerae Japonicae Caulis extract: 0.1 - 3 parts;

[0020] Rehmanniae Radix and / or Rehmanniae Radix extract: 0.1 - 3 parts.

[0021] The inventors of the present application creatively discovered that based on the core formula of the traditional Shouwu Yanshou Dan, by introducing Platycladi Cacumen and tanshinone, Platycladi Cacumen and tanshinone can play a triple synergistic mechanism of "antioxidation - promoting microcirculation - regulating hair follicle cycle" in the core formula of Shouwu Yanshou Dan. Specifically, it is synergistically increasing the inhibition rate of 5α - reductase, thereby being able to achieve high oil control, anti - inflammatory and hair follicle dredging effects, and having good transdermal properties.

[0022] Preferably, the compound medicinal material extract of the present invention is made from the following components: Polygoni Multiflori Radix and / or Polygoni Multiflori Radix extract: 25 - 35 parts; Siegesbeckiae Herba and / or Siegesbeckiae Herba extract: 7 - 9 parts; Cuscuta chinensis and / or Cuscuta chinensis extract: 7 - 9 parts; Mulberry and / or mulberry extract: 7 - 9 parts; Black sesame and / or black sesame extract: 7 - 9 parts; Rosae Laevigatae Fructus and / or Rosae Laevigatae Fructus extract: 7 - 9 parts; Ecliptae Herba and / or Ecliptae Herba extract: 7 - 9 parts; Platycladi Cacumen and / or Platycladi Cacumen extract: 4 - 6 parts; Eucommiae Cortex and / or Eucommiae Cortex extract: 3 - 5 parts; Achyranthis Bidentatae Radix and / or Achyranthis Bidentatae Radix extract: 3 - 5 parts; Ligustri Lucidi Fructus and / or Ligustri Lucidi Fructus extract: 3 - 5 parts; Mori Folium and / or Mori Folium extract: 1 - 3 parts; Lonicerae Japonicae Caulis and / or Lonicerae Japonicae Caulis extract: 1 - 3 parts; Rehmanniae Radix and / or Rehmanniae Radix extract: 1 - 3 parts.

[0023] In the compound medicinal material extract of the present invention, the components can be the medicinal materials with the above-mentioned names, or the extracts of the medicinal materials with the above-mentioned names, or mixtures of the medicinal materials with the above-mentioned names and their extracts; the extracts of the medicinal materials with the above-mentioned names can all be obtained by extracting the medicinal materials with the above-mentioned names through conventional technical means in the art. In some embodiments of the present invention, the compound medicinal material extract is made of the following components: Polygonum multiflorum Thunb.: 25 - 35 parts; Siegesbeckia orientalis L.: 7 - 9 parts; Cuscuta chinensis Lam.: 7 - 9 parts; Morus alba L. fruit: 7 - 9 parts; Sesamum indicum L. seed: 7 - 9 parts; Rosa laevigata Michx.: 7 - 9 parts; Eclipta prostrata L.: 7 - 9 parts; Platycladus orientalis (L.) Franco: 4 - 6 parts; Eucommia ulmoides Oliv.: 3 - 5 parts; Achyranthes bidentata Blume: 3 - 5 parts; Ligustrum lucidum Ait.: 3 - 5 parts; Morus alba L. leaf: 1 - 3 parts; Lonicera japonica Thunb. stem: 1 - 3 parts; Rehmannia glutinosa Libosch.: 1 - 3 parts. In one embodiment of the present invention, the compound medicinal material extract is made of the following components: Polygonum multiflorum Thunb.: 30 parts; Siegesbeckia orientalis L.: 8 parts; Cuscuta chinensis Lam.: 8 parts; Morus alba L. fruit: 8 parts; Sesamum indicum L. seed: 8 parts; Rosa laevigata Michx.: 8 parts; Eclipta prostrata L.: 8 parts; Platycladus orientalis (L.) Franco: 5 parts; Eucommia ulmoides Oliv.: 4 parts; Achyranthes bidentata Blume: 4 parts; Ligustrum lucidum Ait.: 4 parts; Morus alba L. leaf: 2 parts; Lonicera japonica Thunb. stem: 2 parts; Rehmannia glutinosa Libosch.: 2 parts. The Morus alba L. leaf in the present invention is preferably frosted Morus alba L. leaf.

[0024] The Shouwu Yanshou Dan compound composition provided by the present invention preferably includes 0.1 - 0.5 part of tanshinone and 20 - 50 parts of the compound medicinal material extract. In one embodiment of the present invention, the Shouwu Yanshou Dan compound composition of the present invention includes 0.5 part of tanshinone and 40 parts of the compound medicinal material extract. In another embodiment of the present invention, the Shouwu Yanshou Dan compound composition of the present invention includes 0.5 part of tanshinone and 20 parts of the compound medicinal material extract. In another embodiment of the present invention, the Shouwu Yanshou Dan compound composition of the present invention includes 0.5 part of tanshinone and 50 parts of the compound medicinal material extract. In another embodiment of the present invention, the Shouwu Yanshou Dan compound composition of the present invention includes 0.1 part of tanshinone and 30 parts of the compound medicinal material extract.

[0025] In one embodiment of the present invention, the Shouwu Yanshou Dan compound composition provided by the present invention includes 0.5 part of tanshinone and 40 parts of the compound medicinal material extract, and the compound medicinal material extract is made of the following components: Polygonum multiflorum Thunb.: 30 parts; Siegesbeckia orientalis L.: 8 parts; Cuscuta chinensis Lam.: 8 parts; Morus alba L. fruit: 8 parts; Sesamum indicum L. seed: 8 parts; Rosa laevigata Michx.: 8 parts; Eclipta prostrata L.: 8 parts; Platycladus orientalis (L.) Franco: 5 parts; Eucommia ulmoides Oliv.: 4 parts; Achyranthes bidentata Blume: 4 parts; Ligustrum lucidum Ait.: 4 parts; Frosted Morus alba L. leaf: 2 parts; Lonicera japonica Thunb. stem: 2 parts; Rehmannia glutinosa Libosch.: 2 parts.

[0026] In another embodiment of the present invention, the compound composition of Shouwuyanshou Pills provided by the present invention comprises 0.5 parts of tanshinone and 20 parts of compound medicinal material extract, and the compound medicinal material extract is made from the following components: Polygonum multiflorum: 30 parts; Siegesbeckia orientalis: 8 parts; Cuscuta chinensis: 8 parts; Morus alba fruit: 8 parts; Sesamum indicum: 8 parts; Rosa laevigata: 8 parts; Eclipta prostrata: 8 parts; Platycladus orientalis: 5 parts; Eucommia ulmoides: 4 parts; Achyranthes bidentata: 4 parts; Ligustrum lucidum: 4 parts; Mulberry leaf: 2 parts; Lonicera japonica stem: 2 parts; Rehmannia glutinosa: 2 parts.

[0027] In another embodiment of the present invention, the compound composition of Shouwuyanshou Pills provided by the present invention comprises 0.5 parts of tanshinone and 50 parts of compound medicinal material extract, and the compound medicinal material extract is made from the following components: Polygonum multiflorum: 30 parts; Siegesbeckia orientalis: 8 parts; Cuscuta chinensis: 8 parts; Morus alba fruit: 8 parts; Sesamum indicum: 8 parts; Rosa laevigata: 8 parts; Eclipta prostrata: 8 parts; Platycladus orientalis: 5 parts; Eucommia ulmoides: 4 parts; Achyranthes bidentata: 4 parts; Ligustrum lucidum: 4 parts; Mulberry leaf: 2 parts; Lonicera japonica stem: 2 parts; Rehmannia glutinosa: 2 parts.

[0028] In another embodiment of the present invention, the compound composition of Shouwuyanshou Pills provided by the present invention comprises 0.1 parts of tanshinone and 30 parts of compound medicinal material extract, and the compound medicinal material extract is made from the following components: Polygonum multiflorum: 30 parts; Siegesbeckia orientalis: 8 parts; Cuscuta chinensis: 8 parts; Morus alba fruit: 8 parts; Sesamum indicum: 8 parts; Rosa laevigata: 8 parts; Eclipta prostrata: 8 parts; Platycladus orientalis: 5 parts; Eucommia ulmoides: 4 parts; Achyranthes bidentata: 4 parts; Ligustrum lucidum: 4 parts; Mulberry leaf: 2 parts; Lonicera japonica stem: 2 parts; Rehmannia glutinosa: 2 parts.

[0029] The compound medicinal material extract of the present invention is made from each of the above components by the method of water extraction and alcohol precipitation. Specifically, the compound medicinal material extract of the present invention is obtained by the following method: Each of the above components is decocted with 5 - 20 times the weight of water and filtered, and the filtrate obtained by filtration is concentrated to a relative density of 1.10 - 1.15 to obtain a concentrated solution. An alcohol solvent is used to make the alcohol content of the concentrated solution above 70%, and after standing and separating layers, the supernatant is taken to remove the alcohol solvent to obtain the compound medicinal material extract. Specifically, each of the above components is pulverized, decocted with 5 - 20 times the weight of water for 1 - 3 times, each time for 1 h - 2 h, and after decoction, it is filtered. The filtrates obtained by filtration are combined and then concentrated to a relative density of 1.10 - 1.15 to obtain a concentrated solution. An alcohol solvent is added to the concentrated solution to make the alcohol content of the concentrated solution above 70%, and after standing and separating layers, the supernatant is taken to remove the alcohol solvent to obtain the compound medicinal material extract. The alcohol solvent of the present invention is selected from one or more of ethanol, methanol, and butanol, preferably ethanol. The standing time for standing and separating layers in the present invention is 18 h - 30 h. The alcohol content of the concentrated solution in the present invention is preferably 70% - 80%, more preferably 70%.

[0030] The present invention provides the use of the Fleeceflower Root Longevity Pill compound composition described in any one of the above in the preparation of cosmetics. Based on the fact that the Fleeceflower Root Longevity Pill compound composition has high oil control, anti-inflammatory and hair follicle dredging effects, and good transdermal properties, it is very suitable for use in the preparation of cosmetics, especially suitable for use in the preparation of hair care liquids.

[0031] The present invention also provides a hair care liquid, which is made of materials including water, a humectant, a conditioner, an emulsifier and the Fleeceflower Root Longevity Pill compound composition described in any one of the above; the weight ratio of the water, the humectant, the conditioner, the emulsifier, the tanshinone in the Fleeceflower Root Longevity Pill compound composition and the compound medicinal material extract in the Fleeceflower Root Longevity Pill compound composition is (20 - 60):(1 - 30):(0.1 - 1):(1.5 - 2.5):(0.1 - 1):(1 - 50). Preferably, the weight ratio of the water, the humectant, the conditioner, the emulsifier, the tanshinone in the Fleeceflower Root Longevity Pill compound composition and the compound medicinal material extract in the Fleeceflower Root Longevity Pill compound composition is (25 - 56):(20 - 25):(0.2 - 0.8):(1.8 - 2.2):(0.1 - 0.5):(20 - 50). The humectant in the present invention is selected from at least two of glycerol, propylene glycol, butylene glycol, sodium hyaluronate; the emulsifier is selected from one or more of Tween, lecithin, Span, poloxamer.

[0032] The conditioner in the present invention is selected from one or more of vitamins, tetrahydropiperine, menthol, urea, dipotassium glycyrrhizinate; preferably, the conditioner in the present invention is selected from urea and other conditioners, and the other conditioners are selected from one or more of vitamins, tetrahydropiperine, menthol, dipotassium glycyrrhizinate; urea can help the hair care liquid of the present invention construct a "nanoparticle - cutin softener" composite carrier (urea derivative), thereby further improving the transdermal effect of the hair care liquid of the present invention. According to the Franz diffusion cell test of the present invention, the 24 - hour cumulative penetration amount of stilbene glycoside is about 2.7 times the dose of the blank control group (only containing an equal amount of medicinal materials).

[0033] The pH value of the hair care liquid of the present invention is 4.5 - 6.5, preferably 5.5 - 6.5. In some embodiments of the present invention, the pH of the hair care liquid is adjusted by a pH regulator, and the pH regulator is selected from one or more of citric acid, sodium citrate, sodium hydroxide, potassium hydroxide, triethanolamine, preferably selected from citric acid and sodium citrate.

[0034] The present invention also provides a preparation method of the hair care liquid described in any one of the above, including the following steps: homogenizing water, a humectant, a conditioner, an emulsifier and tanshinone, and then adding a compound medicinal material extract thereto, and adjusting the pH to obtain the hair care liquid.

[0035] In certain embodiments of the present invention, water, a humectant, a conditioning agent, an emulsifier, and tanshinone are homogenized, and then an extract of a compound Chinese medicinal material is added thereto, the pH is adjusted, and filtration is carried out through a microporous membrane with a pore size of 0.20 μm to 0.25 μm, preferably through a 0.22-μm microporous membrane, followed by sterilization and vacuum filling to obtain a hair care liquid.

[0036] The homogenization treatment in the present invention is carried out at a rotational speed of 8000 rpm to 15000 rpm. The temperature of the homogenization treatment in the present invention is 40°C to 50°C, preferably 45°C, and the time of the homogenization treatment is 10 min to 15 min. In certain embodiments of the present invention, the homogenization treatment is carried out using a high-speed shear emulsifier. In certain embodiments of the present invention, the sterilization is carried out by pasteurization, specifically by maintaining at 60°C to 65°C for 25 to 35 minutes, preferably for 30 minutes. In certain embodiments of the present invention, the vacuum degree is maintained at -0.04 MPa to -0.06 MPa during the vacuum filling, and the temperature of the vacuum filling is controlled at 25°C to 30°C.

[0037] The present invention provides a Fleeceflower Root and Lycium Fruit Pill compound composition, its application, a hair care liquid, and a preparation method of the hair care liquid. The Fleeceflower Root and Lycium Fruit Pill compound composition provided by the present invention is based on the core formula of the traditional Fleeceflower Root and Lycium Fruit Pill, and Platycladus orientalis leaf and tanshinone are introduced. Platycladus orientalis leaf and tanshinone can play a triple synergistic mechanism of "antioxidation - promoting microcirculation - regulating hair follicle cycle" in the core formula of the Fleeceflower Root and Lycium Fruit Pill, synergistically achieving high oil control, anti-inflammatory, and hair follicle dredging effects, and having good transdermal property. The present invention constructs a "oral to topical" technical system for the Fleeceflower Root and Lycium Fruit Pill for the first time, realizing enhanced efficacy in topical use. The present invention adopts a scalp transdermal promotion technology, enabling active ingredients such as stilbene glycoside to be directly delivered through the hair follicle - sebaceous gland pathway, and the local bioavailability is increased by 2.7 times compared to the blank. In summary, the present invention successfully develops a highly effective, safe, and anti-hair loss synergistic traditional Chinese medicine hair care product by integrating modern extraction technology, a scientific compounding system, and a stable formulation process, with the aid of the traditional Fleeceflower Root and Lycium Fruit Pill compound, providing a new solution to solve the technical problems existing in existing traditional Chinese medicine hair care products. Description of the Drawings

[0038] Figure 1 It is a white light effect diagram of the scalp surface before using the Fleeceflower Root and Lycium Fruit Pill hair care liquid of Example 1;

[0039] Figure 2 It is a white light effect diagram of the scalp surface 28 days after using the Fleeceflower Root and Lycium Fruit Pill hair care liquid of Example 1;

[0040] Figure 3 It is a polarized light effect diagram of the scalp surface before using the Fleeceflower Root and Lycium Fruit Pill hair care liquid of Example 1;

[0041] Figure 4 The polarized light effect diagram of the scalp surface after 28 days of using the Shouwu Yanshou Dan hair care liquid of Example 1;

[0042] Figure 5 The UV effect diagram of the scalp surface before using the Shouwu Yanshou Dan hair care liquid of Example 1;

[0043] Figure 6 The UV effect diagram of the scalp surface after 28 days of using the Shouwu Yanshou Dan hair care liquid of Example 1. Detailed implementation mode

[0044] The present invention discloses a Shouwu Yanshou Dan compound composition, its application, a hair care liquid, and a preparation method of the hair care liquid. Those skilled in the art can draw on the content of this article and appropriately improve the process parameters to achieve it. It should be particularly pointed out that all similar substitutions and modifications are obvious to those skilled in the art, and they are all regarded as included in the present invention. The methods and applications of the present invention have been described through preferred embodiments, and those related can obviously make changes or appropriate alterations and combinations to the methods and applications in this article without departing from the content, spirit, and scope of the present invention to implement and apply the technology of the present invention.

[0045] The following further elaborates on the present invention in combination with examples:

[0046] Example 1

[0047] The compound medicinal material extraction liquid described in the present invention is made from the medicinal materials in the following parts by weight, as shown in Table 1.

[0048] Table 1

[0049]

[0050] Production process of the compound medicinal material extraction liquid: After the above-mentioned medicinal materials are pulverized, they are passed through a 10-mesh sieve, added with 20 times the amount of water and decocted once for 2 hours, the filtrate is filtered and concentrated to a relative density of 1.15, ethanol is added to make the alcohol content reach 70%, and after standing for 24 hours, the supernatant is taken to recover ethanol and concentrated to the ratio of medicinal materials: concentrated liquid = 1:1, thus obtaining the compound medicinal material extraction liquid.

[0051] Configure the cosmetics according to the formula shown in Table 2, which is the Shouwu Yanshou Dan hair care liquid of the present invention.

[0052] Table 2

[0053]

[0054] Production process of the Shouwu Yanshou Dan hair care liquid:

[0055] First step: Heat component A to 85°C, keep warm for 30 minutes, and then cool down to 45°C for standby;

[0056] Step 2: Heat Component B to 85°C for dissolution and then cool it to 45°C. Slowly add the solution from Step 1 and homogenize for 10 minutes at 10,000 rpm and 45°C.

[0057] Step 3: After the material obtained in Step 2 is cooled to room temperature, add Component C and stir for 10 minutes.

[0058] Example 2

[0059] Prepare the Shouwu Yanshou Dan hair care solution according to the formula in Table 3. The remaining operations are the same as those in Example 1, and the compound medicinal material extract is also the compound medicinal material extract in Example 1.

[0060] Table 3

[0061]

[0062] Example 3

[0063] Prepare the Shouwu Yanshou Dan hair care solution according to the formula in Table 4. The homogenization speed is 12,000 rpm. The remaining operations are the same as those in Example 1, and the compound medicinal material extract is also the compound medicinal material extract in Example 1.

[0064] Table 4

[0065]

[0066] Example 4

[0067] Prepare the Shouwu Yanshou Dan hair care solution according to the formula in Table 5. The homogenization speed is 15,000 rpm. The remaining operations are the same as those in Example 1, and the compound medicinal material extract is also the compound medicinal material extract in Example 1.

[0068] Table 5

[0069]

[0070] Comparative Example 1

[0071] Prepare the control group (blank control group) of the Shouwu Yanshou Dan hair care solution according to the formula in Table 6. The remaining operations are the same as those in Example 1, and the compound medicinal material extract is also the compound medicinal material extract in Example 1.

[0072] Table 6

[0073]

[0074] Comparative Example 2

[0075] Prepare the compound medicinal material extract according to the formula in Table 7:

[0076] Table 7

[0077]

[0078] Production process of the compound medicinal material extract: After crushing the above-mentioned medicinal materials, pass them through a 10-mesh sieve, add 20 times the amount of water and decoct for 1 time for 2 hours. Filter the filtrate and concentrate it to a relative density of 1.15. Add ethanol to make the alcohol content reach 70%. After standing for 24 hours, take the supernatant, recover the ethanol and concentrate it to a ratio of medicinal material: concentrated liquid = 1:1 to obtain the compound medicinal material extract.

[0079] Prepare the Shouwu Yanshou Dan hair care liquid according to the formula in Table 8:

[0080] Table 8

[0081]

[0082] Production process of the Shouwu Yanshou Dan hair care liquid: The same as in Example 1.

[0083] Comparative Example 3

[0084] Prepare the compound medicinal material extract according to the formula in Table 9.

[0085] Table 9

[0086]

[0087] The production process of the compound medicinal material extract is the same as that in Example 1.

[0088] Prepare the Shouwu Yanshou Dan hair care liquid according to the formula in Table 10:

[0089] Table 10

[0090]

[0091] Production process of the Shouwu Yanshou Dan hair care liquid: The same as in Example 1.

[0092] Comparative Example 4

[0093] Prepare the compound medicinal material extract according to the formula in Table 11:

[0094] Table 11

[0095]

[0096] Production process of the compound medicinal material extract: After crushing the above-mentioned medicinal materials, pass them through a 10-mesh sieve, add 20 times the amount of water and decoct for 1 time for 2 hours. Filter the filtrate and concentrate it to a relative density of 1.15. Add ethanol to make the alcohol content reach 70%. After standing for 24 hours, take the supernatant, recover the ethanol and concentrate it to a ratio of medicinal material: concentrated liquid = 1:1 to obtain the compound medicinal material extract.

[0097] Prepare the Shouwu Yanshou Dan hair care liquid according to the formula in Table 12:

[0098] Table 12

[0099]

[0100] Production process of Shouwu Yanshou Dan hair care liquid: same as Example 1.

[0101] Example 5

[0102] Determination of the content of stilbene glucoside (TSG): determined with reference to the high performance liquid chromatography method (General Principles 0512, Volume IV of Chinese Pharmacopoeia).

[0103] Chromatographic conditions and system suitability test: using octadecylsilane chemically bonded silica gel as the filler; using acetonitrile - water (25:75) as the mobile phase; the detection wavelength is 320 nm. The number of theoretical plates calculated with reference to the peak of 2,3,5,4'-tetrahydroxystilbene - 2 - O - β - D - glucoside should be not less than 2000.

[0104] Preparation of reference substance solution: Take an appropriate amount of 2,3,5,4'-tetrahydroxystilbene - 2 - O - β - D - glucoside reference substance, accurately weigh it, add dilute ethanol to make a solution containing 0.2 mg per 1 mL, and that is it.

[0105] Preparation of test solution: Take about 0.2 g of the powder of this product (sieved through No. 4 sieve), accurately weigh it, place it in a stoppered conical flask, accurately add 25 mL of dilute ethanol, weigh it, heat under reflux for 30 minutes, cool, weigh it again, make up the lost weight with dilute ethanol, shake well, let it stand, filter the supernatant, and take the continuous filtrate, and that is it.

[0106] Determination method: Accurately pipette 10 μL each of the reference substance solution and the test solution, inject them into the liquid chromatograph for determination, and that is it. The detection results of the content of stilbene glucoside (TSG) are shown in Table 13.

[0107] Table 13

[0108]

[0109] Example 6

[0110] Franz diffusion cell simulation study: Simulate the physiological conditions of human skin through the Franz diffusion cell to evaluate the transdermal penetration characteristics of stilbene glucoside (TSG) of the present invention, including parameters such as cumulative penetration amount, penetration rate and lag time.

[0111] (1) Experimental equipment and materials:

[0112] Diffusion cell system: C0715 type vertical improved Franz diffusion cell, the diameter of the donor chamber is 15 mm, the receptor volume is 7 mL, magnetic stirring system (rotation speed 200 - 400 rpm), constant temperature water bath system.

[0113] Permeable membrane and skin model: Fresh pig skin;

[0114] Skin pretreatment: Hydrate for 60 minutes and remove subcutaneous fat;

[0115] Donor medium: Shouwuyanshou Pills hair care lotion;

[0116] Receptor medium: Phosphate buffer solution (PBS) with pH 7.4, ultrasonic degassing treatment;

[0117] Detection instrument: High performance liquid chromatograph, operated according to Example 5.

[0118] (2) Experimental steps:

[0119] ① Skin model installation:

[0120] Fix the pretreated skin between the donor chamber and the receptor chamber, with the dermal layer facing the receptor chamber, ensuring no air bubbles. Seal with a horseshoe clip and maintain a constant temperature of 32 ± 1 °C.

[0121] ② Donor application and receptor filling:

[0122] Add Shouwuyanshou Pills hair care lotion to the donor chamber and seal the donor chamber to prevent volatilization.

[0123] Inject preheated PBS into the receptor chamber and maintain magnetic stirring (200 rpm).

[0124] ③ Sampling and liquid supplement:

[0125] Time points: 3, 6, 18, 24 hours.

[0126] Each time, 1 mL of receptor fluid is drawn (synchronously supplemented with isothermal and equal - volume PBS), filtered through a 0.22 μm filter membrane and detected by HPLC. The results of the change in the content of TSG in the Franz diffusion cell simulation experiment are shown in Table 14.

[0127] Table 14

[0128]

[0129] Example 7

[0130] Efficacy research - Human trial experiment:

[0131] 17 - inch scalp detector: Based on multi - spectral imaging and image analysis technology, it collects scalp images in layers through three light sources: white light, polarized light, and UV light, comprehensively reflecting the health status of hair.

[0132] Experimental procedures: Ten healthy volunteers were selected and required to keep their scalps unwashed for 24 hours before the experiment to accumulate a certain amount of oil on their hair for convenient image comparison. The detection environmental temperature was controlled at 25°C ± 2°C. Comparative analysis was carried out before and after using the product for 28 days. The sample used in this experiment was the Shouwu Yanshou Dan hair care solution of Example 1.

[0133] Result analysis: The technical verification based on the multispectral imaging analysis system of the present invention shows that the product of the present invention has significant effects in scalp health regulation, specifically manifested as visual improvements in the following three dimensions:

[0134] (1)Characterization analysis in the white light spectral band

[0135] The scalp surface was observed by diffuse reflection imaging technology, as shown in Figure 1 and Figure 2 . Figure 1 Figure Figure 2 is the white light effect diagram of the scalp surface before using the Shouwu Yanshou Dan hair care solution of Example 1, and

[0136] Figure Figure 1 and Figure 2 are the white light effect diagrams of the scalp surface 28 days after using the Shouwu Yanshou Dan hair care solution of Example 1. Analysis of

[0137] and

[0138] shows that a continuous lipid film was formed on the scalp surface in the untreated state (the oil coverage rate reached 82 ± 5%), resulting in a texture resolution lower than 60 Lp / mm. After applying the product for 28 days, the diffuse reflection effect on the hair shaft surface decreased by 43% (p < 0.01), the clarity of the cutin scale structure increased by 2.3 times, and the recognition degree of the sulcus-cutis ridge conformation reached Industry Standard Grade II, confirming its significant sebum adsorption and cutin conditioning functions. Figure 3 and Figure 4 . Figure 3 Figure Figure 4 is the polarized light effect diagram of the scalp surface before using the Shouwu Yanshou Dan hair care solution of Example 1, and

[0139] Figure Figure 3 and Figure 4 are the polarized light effect diagrams of the scalp surface 28 days after using the Shouwu Yanshou Dan hair care solution of Example 1. Analysis of

[0140] (3)UV Fluorescence Response Detection

[0141] The scalp surface was detected by UV fluorescence response, such as Figure 5 and Figure 6 shown. Figure 5 Figure Figure 6 is the UV effect diagram of the scalp surface before using the Shouwu Yanshou Dan hair care liquid of Example 1,

[0142] Analysis Figure 5 and Figure 6 showed that under the excitation wavelength of 365 nm, porphyrin substances in follicular keratotic plugs showed characteristic brick-red fluorescence (excitation cross-section up to 4.3×10 -17 cm²). After 28 days of treatment with this product, the decay constant of fluorescein intensity increased to 0.86 h -1 , and the follicular orifice diameter increased by 38±5 μm, indicating that its ability to dissolve horny plugs reached the clinical standard of keratolytic agents (OECD 428).

[0143] In summary, the experiment proved that this product achieved systematic improvement of the scalp microenvironment through a triple action mechanism (oil control - anti-inflammatory - dredging).

[0144] Example 8

[0145] 5α-Reductase Inhibition Experiment: 5α-reductase is an important enzyme in the human body, mainly present in organs such as the testis, adrenal gland, and prostate. It is responsible for converting testosterone into dihydrotestosterone (DHT), which plays an important role in promoting hair follicle growth. Therefore, there is a certain relationship between 5α-reductase and hair loss. By inhibiting the activity of 5α-reductase to reduce DHT levels can effectively relieve hair loss.

[0146] The process of the 5α-reductase inhibition experiment is as follows:

[0147] (1)The test substances were pretreated for later use; the test substances were the hair care liquids of Examples 1-4 and Comparative Examples 1-4 respectively;

[0148] (2)Reagents were added to the well plate for reaction according to the following method. 200 μL of sample solution was added to each of the sample tube and the sample background, and enzyme preparations were added to the sample tube, sample blank, enzyme reaction tube, and enzyme blank respectively. Then, testosterone (10 ng / mL) solution was added to the sample tube and the enzyme reaction tube. At the same time, reduced coenzyme II solution (250 μM) and phosphate buffer solution were added to the sample tube, sample blank, enzyme reaction tube, and enzyme blank respectively. Finally, it was incubated at 37 °C for 10 min, and the OD values of each well were measured on the machine and the inhibition rate was calculated.

[0149] Inhibition rate = (OD value of control group - OD value of experimental group) / OD value of control group × 100%

[0150] The test results are shown in Table 15. It can be seen from the table that the inhibition rates of Examples 1 to 4 are higher than those of the blank control group and also better than those of the comparative examples, with statistically significant differences (p < 0.05). This shows that the present invention can effectively relieve hair loss.

[0151] Table 15

[0152]

[0153] The above is only the preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution and inventive concept of the present invention, makes equivalent substitutions or changes, and should be covered by the protection scope of the present invention.

Claims

1. A compound composition of Shouwu Yanshou Pills, characterized in that, By weight, it includes 0.1 to 1 part of tanshinone and 1 to 50 parts of compound medicinal material extract, and the compound medicinal material extract is made from the following components: Polygonum multiflorum Thunb. and / or Polygonum multiflorum Thunb. extract: 10 to 40 parts; Siegesbeckia orientalis L. and / or Siegesbeckia orientalis L. extract: 1 to 10 parts; Cuscuta chinensis Lam. and / or Cuscuta chinensis Lam. extract: 1 to 10 parts; Fructus Mori and / or Fructus Mori extract: 1 to 10 parts; Sesamum indicum L. and / or Sesamum indicum L. extract: 1 to 10 parts; Fructus Rosae Laevigatae and / or Fructus Rosae Laevigatae extract: 1 to 10 parts; Eclipta prostrata L. and / or Eclipta prostrata L. extract: 1 to 10 parts; Platycladus orientalis (L.) Franco and / or Platycladus orientalis (L.) Franco extract: 0.5 to 8 parts; Eucommia ulmoides Oliv. and / or Eucommia ulmoides Oliv. extract: 0.5 to 5 parts; Achyranthes bidentata Blume and / or Achyranthes bidentata Blume extract: 0.5 to 5 parts; Ligustrum lucidum Ait. and / or Ligustrum lucidum Ait. extract: 0.5 to 5 parts; Mulberry leaf and / or Mulberry leaf extract: 0.1 to 3 parts; Honeysuckle stem and / or Honeysuckle stem extract: 0.1 to 3 parts; Rehmannia glutinosa Libosch. and / or Rehmannia glutinosa Libosch. extract: 0.1 to 3 parts.

2. The fleece-flower root longevity pill compound composition according to claim 1, wherein The compound medicinal material extract is made from the following components: Polygonum multiflorum Thunb.: 25 to 35 parts; Siegesbeckia orientalis L.: 7 to 9 parts; Cuscuta chinensis Lam.: 7 to 9 parts; Fructus Mori: 7 to 9 parts; Sesamum indicum L.: 7 to 9 parts; Fructus Rosae Laevigatae: 7 to 9 parts; Eclipta prostrata L.: 7 to 9 parts; Platycladus orientalis (L.) Franco: 4 to 6 parts; Eucommia ulmoides Oliv.: 3 to 5 parts; Achyranthes bidentata Blume: 3 to 5 parts; Ligustrum lucidum Ait.: 3 to 5 parts; Mulberry leaf: 1 to 3 parts; Honeysuckle stem: 1 to 3 parts; Rehmannia glutinosa Libosch.: 1 to 3 parts.

3. The compound composition of Shouwu Yanshou Pills according to claim 1, characterized in that, It includes 0.1 to 0.5 part of tanshinone and 20 to 50 parts of compound medicinal material extract.

4. The Fleeceflower Root Life-Extending Elixir compound composition according to claim 1, wherein, It includes 0.5 part of tanshinone and 40 parts of compound medicinal material extract, and the compound medicinal material extract is made from the following components: Polygonum multiflorum Thunb.: 30 parts; Siegesbeckia orientalis L.: 8 parts; Cuscuta chinensis Lam.: 8 parts; Fructus Mori: 8 parts; Sesamum indicum L.: 8 parts; Fructus Rosae Laevigatae: 8 parts; Eclipta prostrata L.: 8 parts; Platycladus orientalis (L.) Franco: 5 parts; Eucommia ulmoides Oliv.: 4 parts; Achyranthes bidentata Blume: 4 parts; Ligustrum lucidum Ait.: 4 parts; Frosted mulberry leaf: 2 parts; Honeysuckle stem: 2 parts; Rehmannia glutinosa Libosch.: 2 parts; Or, it includes 0.5 part of tanshinone and 20 parts of compound medicinal material extract, and the compound medicinal material extract is made from the following components: Polygonum multiflorum Thunb.: 30 parts; Siegesbeckia orientalis L.: 8 parts; Cuscuta chinensis Lam.: 8 parts; Fructus Mori: 8 parts; Sesamum indicum L.: 8 parts; Fructus Rosae Laevigatae: 8 parts; Eclipta prostrata L.: 8 parts; Platycladus orientalis (L.) Franco: 5 parts; Eucommia ulmoides Oliv.: 4 parts; Achyranthes bidentata Blume: 4 parts; Ligustrum lucidum Ait.: 4 parts; Frosted mulberry leaf: 2 parts; Honeysuckle stem: 2 parts; Rehmannia glutinosa Libosch.: 2 parts; Or, it includes 0.5 part of tanshinone and 50 parts of compound medicinal material extract, and the compound medicinal material extract is made from the following components: Polygonum multiflorum Thunb.: 30 parts; Siegesbeckia orientalis L.: 8 parts; Cuscuta chinensis Lam.: 8 parts; Fructus Mori: 8 parts; Sesamum indicum L.: 8 parts; Fructus Rosae Laevigatae: 8 parts; Eclipta prostrata L.: 8 parts; Platycladus orientalis (L.) Franco: 5 parts; Eucommia ulmoides Oliv.: 4 parts; Achyranthes bidentata Blume: 4 parts; Ligustrum lucidum Ait.: 4 parts; Frosted mulberry leaf: 2 parts; Honeysuckle stem: 2 parts; Rehmannia glutinosa Libosch.: 2 parts; Alternatively, it includes 0.1 part of tanshinone and 30 parts of compound medicinal material extract, and the compound medicinal material extract is made from the following components: fleeceflower root: 30 parts; Siegesbeckia orientalis: 8 parts; dodder seed: 8 parts; mulberry: 8 parts; black sesame: 8 parts; Rosa laevigata: 8 parts; Eclipta prostrata: 8 parts; Biota orientalis: 5 parts; Eucommia ulmoides: 4 parts; Achyranthes bidentata: 4 parts; Glossy privet fruit: 4 parts; Frosted mulberry leaf: 2 parts; Honeysuckle stem: 2 parts; Rehmannia glutinosa: 2 parts.

5. The fleece-flower root longevity pill compound composition according to any one of claims 1 to 4, characterized in that The compound medicinal material extract is prepared by the following method: Decoct and filter each of the above components with 5 - 20 times the weight of water, concentrate the filtrate obtained by filtration to a relative density of 1.10 - 1.15 to obtain a concentrated solution, add an alcohol solvent to the concentrated solution to make the alcohol content of the concentrated solution reach more than 70%, let it stand for layering, and take the supernatant to remove the alcohol solvent to obtain the compound medicinal material extract.

6. Use of the fleeceflower root and polygonum multiflorum longevity - enhancing pill compound composition according to any one of claims 1 - 5 in the preparation of cosmetics.

7. Hair care liquid, characterized in that, It is made from materials including water, a humectant, a conditioner, an emulsifier, and the fleeceflower root and polygonum multiflorum longevity - enhancing pill compound composition according to any one of claims 1 - 5; The weight ratio of the water, humectant, conditioner, emulsifier, tanshinone in the fleeceflower root and polygonum multiflorum longevity - enhancing pill compound composition, and the compound medicinal material extract in the fleeceflower root and polygonum multiflorum longevity - enhancing pill compound composition is (20 - 60):(1 - 30):(0.1 - 1):(1.5 - 2.5):(0.1 - 1):(1 - 50).

8. The hair care liquid according to claim 8, wherein, The humectant is selected from at least two of glycerol, propylene glycol, butylene glycol, and sodium hyaluronate; The conditioner is selected from urea and other conditioning agents, and the other conditioning agents are selected from one or more of vitamins, tetrahydropiperine, menthol, and dipotassium glycyrrhizinate; The emulsifier is selected from one or more of Tween, lecithin, Span, and poloxamer.

9. The hair care liquid according to claim 8, characterized in that, The pH value of the hair care liquid is 4.5 - 6.

5.

10. The preparation method of the hair care liquid according to any one of claims 7 to 9, characterized in that, It includes the following steps: Homogenize water, a humectant, a conditioner, an emulsifier, and tanshinone, and then add the compound medicinal material extract thereto, and adjust the pH to obtain the hair care liquid.

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