Method for establishing model of hamster injection sodium taurocholate induced acute pancreatitis

Abstract

The invention provides a method for establishing a model for injecting sodium taurocholate into hamsters to induce acute pancreatitis, which comprises the following operation steps: S1, selecting 4-week-old naria golden hamsters with the weight of 70-80g, and continuously feeding the hamsters with high-fat feed for 28 days. The invention provides a method for establishing a model for injecting sodium taurocholate to induce acute pancreatitis in hamsters, which comprises the following steps of: continuously feeding the narian golden hamsters with high-fat feed for 28 days, measuring the serum triglyceride level through venous plexus blood sampling, confirming that the hypertriglyceridemia exists, and simultaneously injecting sodium taurocholate into the main pancreatic duct to induce acute pancreatitis through puncturing the main pancreatic duct, so that the acute pancreatitis induced by injecting sodium taurocholate to the narian golden hamsters is induced. A 7% sodium taurocholate solution is injected to establish a hypertriglyceridemia acute pancreatitis model, so that metabolic disorder is pre-induced through high fat diet, the pathologic basis of human hypertriglyceridemia is simulated, pancreatic acinus cells are directly damaged by retrograde injection of sodium taurocholate into pancreatic ducts, and the hypertriglyceridemia acute pancreatitis model is obtained. And the disease course of human hypertriglyceridemia acute pancreatitis is accurately represented by dual mechanisms.

Description

Technical Field

[0001] The present invention relates to the field of model establishment, and particularly to a method for establishing a model of acute pancreatitis induced by injecting sodium taurocholate into hamsters. Background Art

[0002] Acute pancreatitis is a common digestive system disease, among which the fatality rate of severe acute pancreatitis is relatively high. Hypertriglyceridemia has now become one of the most common etiologies after cholelithiasis. Severe cases are more likely to occur in patients with hypertriglyceridemic acute pancreatitis, mainly manifested as fulminant multiple organ failure, and the specific mechanism of its pathogenesis is still unclear.

[0003] Animal models of acute pancreatitis are important tools for studying its pathogenesis. Currently, they are mainly rodents, dogs, pigs, and monkeys, among which rats and mice are the most commonly used in rodents.

[0004] However, due to the completely different lipid metabolism between rats and mice and humans, there are great limitations in studying hypertriglyceridemia. Syrian golden hamsters are an ideal animal model for hypertriglyceridemia because their high-density lipoprotein metabolism is similar to that of humans. However, there is currently a lack of a model of acute pancreatitis in Syrian golden hamsters.

[0005] Therefore, it is necessary to provide a method for establishing a model of acute pancreatitis induced by injecting sodium taurocholate into hamsters to solve the above technical problems. Summary of the Invention

[0006] The present invention provides a method for establishing a model of acute pancreatitis induced by injecting sodium taurocholate into hamsters, which solves the problem of the lack of a model of acute pancreatitis in Syrian golden hamsters at present.

[0007] To solve the above technical problems, the method for establishing a model of acute pancreatitis induced by injecting sodium taurocholate into hamsters provided by the present invention includes the following operating steps:

[0008] S1: Select Syrian golden hamsters weighing 70 to 80 grams and 4 weeks old, and continuously feed them with a high-fat diet for 28 days;

[0009] S2: After 4 weeks, take blood from the retroorbital venous plexus to measure the serum triglyceride level to confirm the presence of hypertriglyceridemia. If it is > 500 mg / dL, it is determined that the hypertriglyceridemia modeling is successful;

[0010] S3: Subsequently, fast and withhold water for 4 hours, and anesthetize the hamsters by gas anesthesia;

[0011] S4: After successful anesthesia, place the hamsters in a supine position on the animal operating table, and insert the noses of the hamsters into the inside of the delivery tube for oxygen supply and continuous anesthesia;

[0012] S5: Make a longitudinal incision about 2 cm long in the middle of the midline of the abdomen. Gently lift the duodenum part with forceps, and puncture from the duodenal side opposite to the pancreas towards the pancreas in the area where the pancreas and duodenum meet with a syringe needle. After seeing the tip of the needle enter the main pancreatic duct, slowly inject sodium taurocholate solution at a rate of 0.2 mL / min, a concentration of 7%, and a dose of 200 μL per hamster. Gently place the duodenum back into the abdominal cavity, and suture the abdominal incision with surgical sutures;

[0013] S6: Return the hamster to the cage for natural recovery.

[0014] Preferably, the specific formula of the high-fat feed is: 15% lard + 2% cholesterol + 0.2% sodium cholate + 82.8% ordinary feed.

[0015] Preferably, one end of the delivery pipe is fixedly connected with a sealing sleeve, one end of the sealing sleeve is fixedly connected with a limiting sleeve, and one end of the limiting sleeve is fixedly connected with a support plate.

[0016] Preferably, a first adjusting device is arranged inside the limiting sleeve. The first adjusting device includes two adjusting grooves, two sliding sleeves, two sliding rods, two moving grooves and two moving blocks. The two adjusting grooves are both opened inside the limiting sleeve, the two sliding sleeves are respectively slidably connected inside the two adjusting grooves, the two sliding rods are respectively slidably connected inside the two sliding sleeves, the two moving grooves are both opened inside the limiting sleeve, and the two moving blocks are respectively slidably connected inside the two moving grooves.

[0017] Preferably, a fixing belt is fixedly connected to the surface of the sliding rod, and both ends of the fixing belt are respectively fixedly connected to one side of the surfaces of the two sliding rods.

[0018] Preferably, a fixing device is arranged inside the moving block. The fixing device includes two fixing holes, two fixing pins and two fixing pads. The two fixing holes are respectively opened inside the two moving blocks, the two fixing pins are respectively threadedly connected inside the two fixing holes, and the two fixing pads are respectively rotatably connected to the surfaces of the two fixing pins.

[0019] Preferably, a limiting component is arranged inside the moving block. The limiting component includes two limiting grooves, two limiting blocks and two anti-slip pads. The two limiting grooves are respectively opened inside the two moving blocks, the two limiting blocks are respectively slidably connected inside the two limiting blocks, and one side of each of the two anti-slip pads is fixedly connected to one side of each of the two limiting blocks.

[0020] Preferably, a positioning device is provided inside the support plate. The positioning device includes a positioning hole and a positioning block. The positioning hole is opened inside the support plate, and the positioning block is threadedly connected inside the positioning hole.

[0021] Preferably, support rods are fixedly connected to the tops of the positioning blocks. A second adjusting device is provided inside each of the two support rods. The second adjusting device includes two adjusting holes and two adjusting rods. The two adjusting holes are respectively opened inside the two support rods, and the two adjusting rods are respectively threadedly connected inside the two adjusting holes.

[0022] Preferably, a limiting band is provided on the surface of the adjusting rod. Two ends of the limiting band are respectively rotatably connected to the surfaces of the two adjusting rods.

[0023] Compared with the related art, the method for establishing a model of acute pancreatitis induced by injecting sodium taurocholate in hamsters provided by the present invention has the following beneficial effects:

[0024] The present invention provides a method for establishing a model of acute pancreatitis induced by injecting sodium taurocholate in hamsters. After continuously feeding Syrian golden hamsters with a high-fat diet for 28 days, blood is taken from the venous plexus to measure the serum triglyceride level, which proves the existence of hypertriglyceridemia. At the same time, by puncturing the main pancreatic duct and injecting 7% sodium taurocholate solution in ml, a model of hypertriglyceridemic acute pancreatitis is established. Thus, metabolic disorders are pre-induced by a high-fat diet to simulate the pathological basis of human hypertriglyceridemia. By retrograde injection of sodium taurocholate into the pancreatic duct to directly damage pancreatic acinar cells, the course of human hypertriglyceridemic acute pancreatitis is accurately reproduced by a dual mechanism. This model can stably reproduce core pathological features such as pancreatic edema, inflammatory cell infiltration, and elevated serum lipase, which highly match the phenotypes of clinical patients. Description of the Drawings

[0025] Figure 1 It is a schematic structural diagram of the first embodiment of the method for establishing a model of acute pancreatitis induced by injecting sodium taurocholate in hamsters provided by the present invention;

[0026] Figure 2 is Figure 1 a schematic cross-sectional structural diagram of the limiting sleeve shown;

[0027] Figure 3 is Figure 2 an enlarged schematic diagram of part A shown;

[0028] Figure 4 It is a schematic structural diagram of the second embodiment of the method for establishing a model of acute pancreatitis induced by injecting sodium taurocholate in hamsters provided by the present invention;

[0029] Figure 5 isFigure 4 Schematic enlarged view of part B shown

[0030] Reference numerals in the figure: 1, delivery tube; 2, sealing sleeve; 3, limiting sleeve; 4, support plate; 5, first adjustment device, 51, adjustment groove, 52, sliding sleeve, 53, sliding rod, 54, moving groove, 55, moving block; 6, fixing device, 61, fixing hole, 62, fixing pin, 63, fixing pad; 7, fixing band; 8, limiting assembly, 81, limiting groove, 82, limiting block, 83, anti-slip pad; 9, positioning device, 91, positioning hole, 92, positioning block; 10, support rod; 11, limiting band; 12, second adjustment device, 121, adjustment hole, 122, adjustment rod. Specific implementation mode

[0031] The present invention will be further described below in conjunction with the accompanying drawings and the implementation mode.

[0032] First embodiment

[0033] Please refer to Figure 1 , Figure 2 and Figure 3 , wherein Figure 1 is a schematic structural view of the first embodiment of the method for establishing a model of acute pancreatitis induced by injecting sodium taurocholate into hamsters provided by the present invention; Figure 2 is Figure 1 a schematic cross-sectional structural view of the limiting sleeve shown; Figure 3 is Figure 2 a schematic enlarged view of part A shown. The method for establishing a model of acute pancreatitis induced by injecting sodium taurocholate into hamsters includes the following operating steps:

[0034] S1: Select Syrian golden hamsters weighing 70 to 80 grams and 4 weeks old, and continuously feed them with high-fat feed for 28 days;

[0035] S2: After 4 weeks, take blood from the retroorbital venous plexus to measure the serum triglyceride level, and confirm the presence of hypertriglyceridemia. If > 500 mg / dL, it is determined that the hypertriglyceridemia modeling is successful;

[0036] S3: Then fast and withhold water for 4 hours, and anesthetize the hamsters by gas anesthesia;

[0037] S4: After successful anesthesia, place the hamsters in the supine position on the animal operating table, and insert the noses of the hamsters into the inside of the delivery tube 1 for oxygen supply and continuous anesthesia;

[0038] S5: Make a longitudinal incision about 2 cm long in the middle of the midline of the abdomen. Gently lift the duodenum with forceps, and puncture from the duodenal side opposite to the pancreas towards the pancreas in the area where the pancreas and duodenum meet with a syringe needle. After seeing the tip of the needle enter the main pancreatic duct, inject sodium taurocholate solution at a rate of 0.2 mL / min, a concentration of 7%, and a dose of 200 μL per animal slowly. Gently place the duodenum back into the abdominal cavity, and close the abdominal incision with surgical sutures;

[0039] S6: Return the hamster to the cage for natural recovery.

[0040] The specific formula of the high-fat diet is: 15% lard + 2% cholesterol + 0.2% sodium cholate + 82.8% ordinary feed.

[0041] The core pathophysiological mechanism of HTGP (hypertriglyceridemia-induced pancreatic lipotoxicity) was simulated by high-fat feeding, and the injection of sodium taurocholate directly damaged the pancreas. The dual effects may be closer to the progression of human diseases. Compared with a single induction method (such as only chemical injury or only diet induction), this model has more advantages in simulating the pathological mechanism.

[0042] One end of the conveying pipe 1 is fixedly connected with a sealing sleeve 2, one end of the sealing sleeve 2 is fixedly connected with a limiting sleeve 3, and one end of the limiting sleeve 3 is fixedly connected with a support plate 4.

[0043] A first adjusting device 5 is arranged inside the limiting sleeve 3. The first adjusting device 5 includes two adjusting grooves 51, two sliding sleeves 52, two sliding rods 53, two moving grooves 54 and two moving blocks 55. The two adjusting grooves 51 are both opened inside the limiting sleeve 3. The two sliding sleeves 52 are respectively slidably connected inside the two adjusting grooves 51. The two sliding rods 53 are respectively slidably connected inside the two sliding sleeves 52. The two moving grooves 54 are both opened inside the limiting sleeve 3. The two moving blocks 55 are respectively slidably connected inside the two moving grooves 54.

[0044] A fixing belt 7 is fixedly connected to the surface of the sliding rod 53, and two ends of the fixing belt 7 are respectively fixedly connected to one side of the surfaces of the two sliding rods 53.

[0045] The two sliding rods 53 are respectively slidably connected inside the two adjusting grooves 51, and the sliding rod 53 is used to adjust the position and height of one end of the fixing belt 7.

[0046] The two moving grooves 54 are respectively arranged on one side of the two adjusting grooves 51, and one side is a through groove, which is used to drive the fixing pin 62 to move to one side when the moving block 55 moves to one side.

[0047] The fixing band 7 is a rubber band made of rubber material and is used to limit the hamster.

[0048] A fixing device 6 is arranged inside the moving block 55. The fixing device 6 includes two fixing holes 61, two fixing pins 62 and two fixing pads 63. The two fixing holes 61 are respectively opened inside the two moving blocks 55. The two fixing pins 62 are respectively threadedly connected inside the two fixing holes 61. The two fixing pads 63 are respectively rotatably connected to the surfaces of the two fixing pins 62.

[0049] The fixing pin 62 is a bolt, and the fixing hole 61 is a threaded hole adapted to the fixing pin 62, and is used to limit the sliding rod 53 and the moving block 55 through the fixing pin 62.

[0050] One end of the fixing pin 62 is fixedly connected with a rotating handle for facilitating the rotation of the fixing pin 62.

[0051] A limiting component 8 is arranged inside the moving block 55. The limiting component 8 includes two limiting grooves 81, two limiting blocks 82 and two anti-slip pads 83. The two limiting grooves 81 are respectively opened inside the two moving blocks 55. The two limiting blocks 82 are respectively slidably connected inside the two limiting grooves 81. One sides of the two anti-slip pads 83 are respectively fixedly connected to one sides of the two limiting blocks 82.

[0052] Limiting grooves 81 are also opened inside the two sliding sleeves 52, and the limiting blocks 82 are slidably connected inside the limiting grooves 81.

[0053] The limiting block 82 is a semi-circular block adapted to the sliding rod 53. One end of the limiting block 82 is rotatably connected to one end of the fixing pin 62, and is used to drive the limiting block 82 to move to one side inside the limiting groove 81 when the fixing pin 62 is rotated to one side. When the surface of the limiting block 82 contacts the surface of the sliding rod 53, the sliding rod 53 is limited.

[0054] The limiting block 82 is used to increase the contact between the fixing pin 62 and the sliding rod 53, thereby increasing the limiting effect.

[0055] The anti-slip pad 83 is made of rubber material and is used to increase the friction between the limiting block 82 and the sliding rod 53, thereby increasing the limiting effect.

[0056] When in use, after placing the nose of the hamster inside the sealing sleeve 2, the fixing band 7 can be placed at the chin position of the hamster.

[0057] When it is necessary to adjust the position and height of the fixing band 7, by rotating the fixing pin 62 to one side, so that while the fixing pin 62 moves to one side inside the fixing hole 61, one end of the fixing pin 62 separates from the surface of the sliding rod 53, and drives one side of the fixing pad 63 to separate from the surface of the limit sleeve 3.

[0058] While moving the sliding rod 53 upward and to one side, so that the sliding rod 53 moves upward inside the sliding sleeve 52, one end of the limit band 11 moves upward. At the same time, when the sliding rod 53 moves to one side, it drives the sliding sleeve 52 connected to the moving block 55 to move to one side inside the moving groove 54 to a suitable position. Then, by rotating the fixing pin 62 back to one side, one end of the fixing pin 62 contacts the surface of the sliding rod 53 to limit the sliding rod 53. At the same time, after one side of the fixing pad 63 contacts the surface of the limit sleeve 3, it limits the moving block 55.

[0059] The working principle of the method for establishing a hamster model of acute pancreatitis induced by injecting sodium taurocholate provided by the present invention is as follows:

[0060] In use, select Syrian golden hamsters weighing 70 to 80 grams and 4 weeks old, and continuously feed them with high-fat feed for 28 days;

[0061] After 4 weeks, take blood from the retroorbital venous plexus to measure the serum triglyceride level to confirm the existence of hypertriglyceridemia;

[0062] Subsequently, fast and withhold water for 4 hours, and anesthetize the hamsters by gas anesthesia;

[0063] After successful anesthesia, place the hamsters in the supine position on the animal operating table, and insert the noses of the hamsters into the inside of the delivery tube 1 for oxygen supply and continuous anesthesia;

[0064] Make a longitudinal incision about 2 cm long in the middle of the midline of the abdomen. Gently lift the duodenum part with forceps, puncture from the duodenum on the opposite side of the junction area of the pancreas and duodenum towards the pancreas with a syringe needle. After seeing the tip of the needle enter the main pancreatic duct, inject 7% sodium taurocholate solution in ml. Gently put the duodenum back into the abdominal cavity, and suture the abdominal incision with surgical sutures;

[0065] Return the hamsters to the cage for natural recovery.

[0066] Compared with the related technologies, the method for establishing a hamster model of acute pancreatitis induced by injecting sodium taurocholate provided by the present invention has the following beneficial effects:

[0067] The present invention provides a method for establishing a model of acute pancreatitis induced by injecting sodium taurocholate into hamsters. After continuously feeding Syrian golden hamsters with a high-fat diet for 28 days, blood is taken from the venous plexus to measure the serum triglyceride level, and hypertriglyceridemia is confirmed. At the same time, by puncturing the main pancreatic duct and injecting 7% sodium taurocholate solution per milliliter, a model of hypertriglyceridemic acute pancreatitis is established. Thus, metabolic disorders are pre-induced by a high-fat diet to simulate the pathological basis of human hypertriglyceridemia, and pancreatic acinar cells are directly damaged by retrograde injection of sodium taurocholate into the pancreatic duct. The dual mechanisms accurately reproduce the course of human hypertriglyceridemic acute pancreatitis. This model can stably reproduce core pathological features such as pancreatic edema, inflammatory cell infiltration, and elevated serum lipase, which highly coincide with the phenotypes of clinical patients.

[0068] Second Embodiment

[0069] Please refer to Figure 2 、 Figure 3 and Figure 5 Based on the method for establishing a model of acute pancreatitis induced by injecting sodium taurocholate into hamsters provided in the first embodiment of this application, the second embodiment of this application proposes another method for establishing a model of acute pancreatitis induced by injecting sodium taurocholate into hamsters. The second embodiment is merely a preferred manner of the first embodiment, and the implementation of the second embodiment will not affect the independent implementation of the first embodiment.

[0070] Specifically, the difference in the method for establishing a model of acute pancreatitis induced by injecting sodium taurocholate into hamsters provided in the second embodiment of this application is that in the method for establishing a model of acute pancreatitis induced by injecting sodium taurocholate into hamsters, a positioning device 9 is provided inside the support plate 4. The positioning device 9 includes a positioning hole 91 and a positioning block 92. The positioning hole 91 is opened inside the support plate 4, and the positioning block 92 is threadedly connected inside the positioning hole 91.

[0071] The positioning block 92 is a threaded block, the positioning hole 91 is a threaded hole adapted to the positioning block 92, and a plurality of positioning holes 91 are opened inside the support plate 4 to facilitate the installation and position adjustment of the support rod 10.

[0072] Support rods 10 are fixedly connected to the tops of the positioning blocks 92. Second adjustment devices 12 are provided inside both of the support rods 10. The second adjustment devices 12 include two adjustment holes 121 and two adjustment rods 122. The two adjustment holes 121 are respectively opened inside the two support rods 10, and the two adjustment rods 122 are respectively threadedly connected inside the two adjustment holes 121.

[0073] The adjusting rod 122 is a threaded rod, and the adjusting hole 121 is a threaded hole adapted to the adjusting rod 122, which is used to move the adjusting rod 122 inside the adjusting hole 121 when the adjusting rod 122 rotates to one side.

[0074] The threads provided on the surface of the adjusting rod 122 are opposite to the threads on the surface of the positioning block 92, so that when the adjusting rod 122 rotates to one side, it will not drive the support rod 10 to rotate to one side, causing looseness between the positioning block 92 and the positioning hole 91.

[0075] A limiting band 11 is provided on the surface of the adjusting rod 122, and both ends of the limiting band 11 are respectively rotatably connected to the surfaces of the two adjusting rods 122.

[0076] The adjusting rod 122 is used to adjust the height of one end of the limiting band 11 at different levels.

[0077] The limiting band 11 is a rubber band made of rubber material and is used to limit the hamster.

[0078] A rotating handle is fixedly connected to one end of the adjusting rod 122, which is used to facilitate the rotation of the adjusting rod 122.

[0079] The working principle of the method for establishing a model of acute pancreatitis induced by injecting sodium taurocholate into hamsters provided by the present invention is as follows:

[0080] When in use, by rotating the support rod 10 to one side, the positioning block 92 is driven to rotate to one side inside the positioning hole 91, so that after the positioning block 92 moves and separates inside the positioning hole 91, after moving the support rod 10 to a suitable position, aligning the positioning block 92 with the positioning hole 91, and then rotating the support rod 10 to one side, the positioning block 92 can be driven to move downward to a suitable position inside the positioning hole 91.

[0081] When the height of the limiting band 11 needs to be adjusted, by rotating the two adjusting rods 122 to one side, the two adjusting rods 122 can move upward inside the two adjusting holes 121 respectively to adjust the height of both ends of the limiting band 11.

[0082] By placing the limiting band 11 under the armpits of the front legs of the hamster, the hamster can be limited.

[0083] Compared with the related art, the method for establishing a model of acute pancreatitis induced by injecting sodium taurocholate into hamsters provided by the present invention has the following beneficial effects:

[0084] The present invention provides a method for establishing a model of acute pancreatitis induced by injecting sodium taurocholate into hamsters. By means of a positioning device 9 cooperating with a second adjusting device 12, a limiting belt 11 is moved to a suitable position to limit the axilla under the forelimbs of the hamsters, thereby increasing the stability of the hamsters during the operation.

[0085] The above are only embodiments of the present invention, and do not thus limit the patent scope of the present invention. Any equivalent structural or equivalent process transformation made by using the content of the specification and drawings of the present invention, or directly or indirectly applied in other related technical fields, shall similarly be included within the patent protection scope of the present invention.

Claims

1. A method for establishing a model of acute pancreatitis induced by sodium taurocholate injection in hamsters, characterized in that, Including: The following operating steps: S1: Select Syrian golden hamsters at 4 weeks old with a body weight of 70 to 80 grams, and continuously feed them with a high-fat diet for 28 days; S2: After 4 weeks, take blood from the retroorbital venous plexus to measure the serum triglyceride level to confirm the presence of hypertriglyceridemia. If it is >500 mg / dL, it is determined that the hypertriglyceridemia modeling is successful; S3: Subsequently, fast and withhold water for 4 hours, and anesthetize the hamsters using gas anesthesia; S4: After successful anesthesia, place the hamsters in a supine position on the animal operating table, and insert the noses of the hamsters into the inside of the delivery tube for oxygen supply and continuous anesthesia; S5: Make a longitudinal incision about 2 cm long in the middle of the midline of the abdomen. Gently lift the duodenum part with forceps, and puncture from the duodenal side opposite to the junction area of the pancreas and duodenum towards the pancreas with a syringe needle. After seeing the tip of the needle enter the main pancreatic duct, inject the sodium taurocholate solution at a rate of 0.2 mL / min, a concentration of 7%, and a dose of 200 μL / animal. Gently place the duodenum back into the abdominal cavity, and suture the abdominal incision; S6: Return the hamsters to the cage for natural recovery.

2. The method for establishing a model of sodium taurocholate-induced acute pancreatitis in hamsters according to claim 1, wherein The specific formula of the high-fat diet is: 15% lard + 2% cholesterol + 0.2% sodium cholate + 82.8% ordinary feed.

3. The method for establishing a model of acute pancreatitis induced by sodium taurocholate injection in hamsters according to claim 1, characterized in that, One end of the delivery tube is fixedly connected with a sealing sleeve, one end of the sealing sleeve is fixedly connected with a limiting sleeve, and one end of the limiting sleeve is fixedly connected with a support plate.

4. The method for establishing a model of acute pancreatitis induced by injecting sodium taurocholate into hamsters according to claim 3, wherein A first adjusting device is arranged inside the limiting sleeve. The first adjusting device includes two adjusting grooves, two sliding sleeves, two sliding rods, two moving grooves and two moving blocks. The two adjusting grooves are both opened inside the limiting sleeve. The two sliding sleeves are respectively slidably connected inside the two adjusting grooves. The two sliding rods are respectively slidably connected inside the two sliding sleeves. The two moving grooves are both opened inside the limiting sleeve. The two moving blocks are respectively slidably connected inside the two moving grooves.

5. The method for establishing a model of acute pancreatitis induced by sodium taurocholate injection in hamsters according to claim 4, characterized in that, A fixing belt is fixedly connected to the surface of the sliding rod, and both ends of the fixing belt are respectively fixedly connected to one side of the surfaces of the two sliding rods.

6. The method for establishing a model of acute pancreatitis induced by injecting sodium taurocholate into hamsters according to claim 4, wherein A fixing device is arranged inside the moving block. The fixing device includes two fixing holes, two fixing pins and two fixing pads. The two fixing holes are respectively opened inside the two moving blocks. The two fixing pins are respectively threadedly connected inside the two fixing holes. The two fixing pads are respectively rotatably connected to the surfaces of the two fixing pins.

7. The method for establishing a model of acute pancreatitis induced by sodium taurocholate injection in hamsters according to claim 6, characterized in that, A limiting component is arranged inside the moving block. The limiting component includes two limiting grooves, two limiting blocks and two anti-slip pads. The two limiting grooves are respectively opened inside the two moving blocks. The two limiting blocks are respectively slidably connected inside the two limiting blocks. One side of the two anti-slip pads is respectively fixedly connected to one side of the two limiting blocks.

8. The method for establishing a model of acute pancreatitis induced by injecting sodium taurocholate into hamsters according to claim 3, characterized in that, A positioning device is arranged inside the support plate. The positioning device includes a positioning hole and a positioning block. The positioning hole is opened inside the support plate, and the positioning block is threadedly connected inside the positioning hole.

9. The method for establishing a model of sodium taurocholate-induced acute pancreatitis in hamsters according to claim 8, wherein Support rods are fixedly connected to the tops of the positioning blocks. Second adjusting devices are arranged inside both of the support rods. The second adjusting device includes two adjusting holes and two adjusting rods. The two adjusting holes are respectively formed inside the two support rods, and the two adjusting rods are respectively threadedly connected to the interiors of the two adjusting holes.

10. The method for establishing a model of acute pancreatitis induced by injecting sodium taurocholate into hamsters according to claim 9, wherein, A limiting band is arranged on the surface of the adjusting rod, and two ends of the limiting band are respectively rotatably connected to the surfaces of the two adjusting rods.

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