A non-invasive medical aesthetic repair and enhancement composition and its application

Abstract

This invention relates to a non-invasive aesthetic medicine repair and enhancement composition and its application. The composition comprises the following components: oat beta-glucan, oat kernel extract, holy basil leaf extract, hops extract, and Brazil nut seed extract, wherein the weight ratio of oat beta-glucan, oat kernel extract, holy basil leaf extract, hops extract, and Brazil nut seed extract is (0.11-3):(0.1-2):(1-5):(0.1-1):(0.2-2). Through a multi-dimensional synergistic mechanism, the composition of this invention strengthens the skin barrier and increases tolerance before surgery, and repairs and enhances postoperative effects to prevent pigmentation and acne, achieving a closed-loop management of the entire cycle of non-invasive aesthetic medicine: "preoperative defense - postoperative repair - effect maintenance."

Description

Technical Field

[0001] This invention relates to the technical field of cosmetics, specifically to a non-invasive medical aesthetic repair and enhancement composition and its application. Background Technology

[0002] Medical aesthetics (cosmetic surgery) refers to methods that improve appearance and body shape through medical techniques, and is divided into two categories: invasive and non-invasive. Non-invasive medical aesthetics (light medical aesthetics) include procedures such as IPL (Intense Pulsed Light) skin rejuvenation, radiofrequency lifting, hyaluronic acid injections, and Thermage. These primarily use light, electricity, heat, or injections to act on the skin's surface or dermis, requiring no surgical incisions and having a short recovery period. Invasive medical aesthetics, such as fractional laser treatments, microneedling, and thread lifting, cause skin damage through minimally invasive or surgical methods to promote collagen regeneration. These procedures have a longer recovery period and require specialized care, making it impossible to use regular skincare products. Although non-invasive procedures do not involve open wounds, the photothermal effects may damage the skin barrier function, leading to dryness, redness, or photosensitive pigmentation. For example, IPL skin rejuvenation reduces the skin's moisturizing ability, requiring post-operative hydration to repair the barrier; radiofrequency treatment may trigger a temporary inflammatory reaction, requiring anti-inflammatory care. Pre-operative care for medical aesthetic procedures can ensure improved skin tolerance and reduce the risk of irritation during the procedure. Aesthetic medicine stimulates skin regeneration through a "break-and-build" mechanism. Post-operative repair can prolong the therapeutic effect and prevent complications such as breakouts caused by bacterial imbalance, post-inflammatory hyperpigmentation, and skin sensitivity.

[0003] Current development of post-cosmetic surgery repair products largely focuses on passive repair in a single post-operative stage. For example, combinations of hyaluronic acid, collagen, or repair plant extracts are used to alleviate post-operative redness and dryness through moisturizing or anti-inflammatory effects. However, these products generally suffer from the following drawbacks: First, existing technologies lack proactive strengthening of the pre-operative skin barrier, making the skin prone to acute inflammatory reactions due to thermal effects or mechanical stimulation during cosmetic procedures, affecting post-operative recovery efficiency. Second, most products only target epidermal moisturizing or superficial anti-inflammatory effects, lacking sufficient synergistic effects and failing to simultaneously regulate dermal collagen metabolism, microecological balance, and cell connection structure, leading to secondary problems such as recurring pigmentation and acne breakouts. Third, traditional formulas do not address pre-operative barrier strengthening and do not consider that establishing skin tolerance beforehand is also crucial for enhancing the effectiveness of cosmetic procedures and reducing irritation. Summary of the Invention

[0004] The purpose of this invention is to overcome the shortcomings of the prior art and provide a non-invasive medical aesthetic repair and enhancement composition and its application.

[0005] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0006] In a first aspect, the present invention provides a non-invasive medical aesthetic repair and enhancement composition, comprising the following components: oat beta-glucan, oat kernel extract, holy basil leaf extract, hops extract and Brazil nut seed extract, wherein the weight ratio of oat beta-glucan, oat kernel extract, holy basil leaf extract, hops extract and Brazil nut seed extract is (0.11-3):(0.1-2):(1-5):(0.1-1):(0.2-2).

[0007] The composition of this invention achieves non-invasive, full-cycle medical aesthetic care through multi-dimensional synergistic effects, as detailed below:

[0008] Oat β-glucan forms a dynamic protective film in the epidermis with its unique glycosidic bond structure. It promotes the regeneration of the stratum corneum and the synthesis of collagen in the dermis by activating cell signaling pathways, thereby enhancing the skin's ability to resist external stimuli. At the same time, it works synergistically with oat kernel extract to construct a biomimetic lipid network, optimizing the mechanical strength and water-locking function of the barrier.

[0009] Oat kernel extract reduces the release of key inflammatory mediators by inhibiting inflammatory signal transduction pathways. Its natural lipid components and oat β-glucan molecules form a three-dimensional barrier structure through hydrophobic interactions, further consolidating the integrity of the stratum corneum and inhibiting the proliferation of pathogenic bacteria at the microecological level, thus reducing the risk of postoperative adverse reactions.

[0010] Holy basil leaf extract directly reduces melanin production by inhibiting tyrosinase activity and the MITF pathway through its active ingredients (ursolic acid, eugenol, etc.); it also simultaneously blocks inflammatory pathways and scavenges free radicals, preventing inflammation and oxidative stress from activating melanocytes. Furthermore, its ability to promote barrier protein expression synergistically strengthens the skin's physical defenses with oat beta-glucan, reducing the risk of pigmentation induced by external stimuli.

[0011] Hops extract targets and regulates the balance of skin surface flora and the activity of nerve receptors, reduces the excessive release of inflammatory factors, and enhances the expression of intercellular connection proteins, forming a dual protection of microbial and physical barriers, significantly reducing the probability of secondary problems such as postoperative acne.

[0012] Brazil nut seed extract promotes the secretion of protective lipids in the epidermis by activating lipid synthesis-related pathways, accelerates the differentiation and maturation of keratinocytes, and enhances the mechanical strength of tight junctions in the epidermis by strengthening the stability of cytoskeletal proteins and junctional structures, thus forming a closed-loop repair system from cell regeneration to barrier strengthening.

[0013] The components in the composition of this invention work through a phased mechanism to build a defensive barrier before surgery, increase skin tolerance, prevent strong irritation during surgery, promote synergistic regeneration after surgery, enhance the medical aesthetic effect, prevent inflammatory pigmentation and acne breakouts, and form a systematic solution covering the entire medical aesthetic cycle.

[0014] Preferably, the weight ratio of oat β-glucan, oat kernel extract, holy basil leaf extract, hop extract and Brazil nut seed extract is (0.5-2):(1-2):(1-3):(0.1-1.2):(0.5-1.2).

[0015] Preferably, the weight ratio of oat β-glucan, oat kernel extract, holy basil leaf extract, hop extract and Brazil nut seed extract is (1-1.2):(1.5-2):(1-2):(0.1-0.8):(0.8-1).

[0016] In a second aspect, the present invention provides the application of the non-invasive medical aesthetically applicable repair and enhancement composition described in the first aspect in the preparation of cosmetics.

[0017] Preferably, the cosmetic is a toner, lotion, cream, mask, serum, or spray, and the amount of the composition added is 1%-5% of the total weight of the cosmetic.

[0018] Thirdly, the present invention provides an essence comprising the following ingredients by weight percentage: 1%-5% of the non-invasive medical aesthetic repair and enhancement composition described in the first aspect, 0.05%-0.5% of a thickener, 3%-10% of a moisturizer, 0.5%-3% of a preservative, and 0.01%-0.3% of a pH adjuster, with the balance being deionized water.

[0019] Preferably, the thickener includes at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, and sclerotium gum.

[0020] Preferably, the moisturizer comprises at least one of allantoin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, D-panthenol, sodium hyaluronate, 1,2-butanediol, glycerin, budding stalk polysaccharide, and ceramide.

[0021] Preferably, the pH adjuster includes at least one of arginine, tromethamine, and disodium EDTA.

[0022] Preferably, the preservative includes at least one of 1,3-propanediol, 1,2-hexanediol, and p-hydroxyacetophenone.

[0023] Fourthly, the present invention provides a method for preparing the essence described in the third aspect, comprising the following steps:

[0024] S1. Mix the humectant, thickener and part of the deionized water, homogenize at 75-85℃, then add the preservative and stir evenly to obtain the mixture.

[0025] S2. Once the temperature of the mixture in S1 drops to 35-45℃, add the components of the non-invasive medical aesthetic repair and enhancement composition and the remaining deionized water, stir evenly, and finally add a pH adjuster to adjust the pH to obtain the essence.

[0026] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0027] The non-invasive medical aesthetic repair and enhancement composition of the present invention comprises oat β-glucan, oat kernel extract, holy basil leaf extract, hops extract, and Brazil nut seed extract. These components synergistically promote collagen production, enhance dermal resilience, improve barrier defense and tolerance to resist damage before surgery, repair skin damage after surgery, reduce irritation sensitivity and promote wound healing, thereby enhancing the medical aesthetic effect. At the same time, it can also inhibit neutrophil aggregation and suppress postoperative inflammation-induced hyperpigmentation and acne breakouts. Attached Figure Description

[0028] Figure 1 The improvement of facial redness before and after cosmetic procedures is shown in Application Example 1 and Comparative Application Example 6. Detailed Implementation

[0029] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0030] The sources of the raw materials used in the following examples and comparative examples are as follows:

[0031] Oat beta-glucan: Manufacturer is Beijing Dongfang Miaosen Biotechnology Co., Ltd., and the product name is Miaokexiu;

[0032] Oat kernel extract: Manufacturer: Symrise Fragrance & Flavor (Nantong) Co., Ltd.; Product name: Avena;

[0033] Holy basil leaf extract: manufactured by Shaanxi Sinote Biotechnology Co., Ltd.;

[0034] Hops extract: manufactured by BASF, trade name Senseryn TM ;

[0035] Brazilian fruit seed extract: manufactured by Clariant Chemicals (China) Co., Ltd., trade name EthienceProtect.

[0036] Unless otherwise specified, all other materials and reagents used in the examples are commercially available.

[0037] Example 1

[0038] A non-invasive medical aesthetic repair and enhancement composition comprising oat beta-glucan, oat kernel extract, holy basil leaf extract, hops extract, and Brazil nut seed extract in a weight ratio of 1.1:1.8:1.3:0.5:0.9, wherein the total mass fraction of oat beta-glucan, oat kernel extract, holy basil leaf extract, hops extract, and Brazil nut seed extract is 100 parts.

[0039] Example 2

[0040] A non-invasive medical aesthetic repair and enhancement composition comprising oat beta-glucan, oat kernel extract, holy basil leaf extract, hops extract, and Brazil nut seed extract in a weight ratio of 1:2:2:0.8:0.8, wherein the total mass fraction of oat beta-glucan, oat kernel extract, holy basil leaf extract, hops extract, and Brazil nut seed extract is 100 parts.

[0041] Example 3

[0042] A non-invasive medical aesthetic repair and enhancement composition comprising oat beta-glucan, oat kernel extract, holy basil leaf extract, hops extract, and Brazil nut seed extract in a weight ratio of 1.2:1.5:1:0.1:1, wherein the total mass fraction of oat beta-glucan, oat kernel extract, holy basil leaf extract, hops extract, and Brazil nut seed extract is 100 parts.

[0043] Example 4

[0044] A non-invasive medical aesthetic repair and enhancement composition comprising oat beta-glucan, oat kernel extract, holy basil leaf extract, hops extract, and Brazil nut seed extract in a weight ratio of 0.5:1:3:0.1:0.5, wherein the total mass fraction of oat beta-glucan, oat kernel extract, holy basil leaf extract, hops extract, and Brazil nut seed extract is 100 parts.

[0045] Example 5

[0046] A non-invasive medical aesthetic repair and enhancement composition comprising oat beta-glucan, oat kernel extract, holy basil leaf extract, hops extract, and Brazil nut seed extract in a weight ratio of 2:2:1:1.2:1.2, wherein the total mass fraction of oat beta-glucan, oat kernel extract, holy basil leaf extract, hops extract, and Brazil nut seed extract is 100 parts.

[0047] Example 6

[0048] A non-invasive medical aesthetic repair and enhancement composition comprising oat beta-glucan, oat kernel extract, holy basil leaf extract, hops extract, and Brazil nut seed extract in a weight ratio of 0.11:0.1:5:1:2, wherein the total mass fraction of oat beta-glucan, oat kernel extract, holy basil leaf extract, hops extract, and Brazil nut seed extract is 100 parts.

[0049] Example 7

[0050] A non-invasive medical aesthetic repair and enhancement composition comprising oat beta-glucan, oat kernel extract, holy basil leaf extract, hops extract, and Brazil nut seed extract in a weight ratio of 3:2:1:0.1:0.2, wherein the total mass fraction of oat beta-glucan, oat kernel extract, holy basil leaf extract, hops extract, and Brazil nut seed extract is 100 parts.

[0051] Comparative Example 1

[0052] The difference between Comparative Example 1 and Example 1 is that oat β-glucan was not added to the composition, and oat kernel extract, holy basil leaf extract, hops extract and Brazil nut seed extract in a weight ratio of 1.8:1.3:0.5:0.9 were used to make up for the missing amount.

[0053] Comparative Example 2

[0054] The difference between Comparative Example 2 and Example 1 is that oat kernel extract was not added to the composition, and oat β-glucan, holy basil leaf extract, hop extract and Brazil nut seed extract in a weight ratio of 1.1:1.3:0.5:0.9 were used to make up for the missing amount.

[0055] Comparative Example 3

[0056] The difference between Comparative Example 3 and Example 1 is that the composition does not contain holy basil leaf extract, and the missing amount is made up by oat β-glucan, oat kernel extract, hop extract and Brazil nut seed extract in a weight ratio of 1.1:1.8:0.5:0.9.

[0057] Comparative Example 4

[0058] The difference between Comparative Example 4 and Example 1 is that: no hop extract was added to the composition, and oat β-glucan, oat kernel extract, holy basil leaf extract and Brazil nut seed extract in a weight ratio of 1.1:1.8:1.3:0.9 were used to make up for the missing amount.

[0059] Comparative Example 5

[0060] The difference between Comparative Example 5 and Example 1 is that Brazil nut seed extract was not added to the composition, and oat β-glucan, oat kernel extract, holy basil leaf extract and hop extract in a weight ratio of 1.1:1.8:1.3:0.5 were used to make up for the missing amount.

[0061] Test Example 1: Test to Promote Collagen Regeneration and Barrier Function

[0062] The compositions of Examples 1-7 and Comparative Examples 1-5 were set as 12 test sample groups, and the experiments were carried out according to the following steps:

[0063] 1. Cell lines and culture conditions

[0064] Cell lines: human immortalized keratinocytes (HaCaT, catalog number YN2020, Shanghai Yaji Biotechnology Co., Ltd.) and human dermal fibroblasts (HSF, catalog number C1101P, Guangzhou Genio Biotechnology Co., Ltd.). All cells were identified by STR and were free of mycoplasma contamination.

[0065] Culture conditions: 37±0.5℃ constant temperature incubator, saturated humidity (95%±2%), 5% CO2 environment.

[0066] 2. Sample processing

[0067] Sample processing solution preparation: The compositions of Examples 1-7 and Comparative Examples 1-5 were dissolved in dimethyl sulfoxide (DMSO, final concentration ≤0.1%), and adjusted to a final concentration of 0.1% (v / v) by gradient dilution (DMEM high glucose medium containing 10% fetal bovine serum). The solutions were then aseptically filtered through a 0.22 μm filter membrane to obtain the sample processing solutions for each group.

[0068] 3. Cell resuscitation and culture

[0069] Logarithmically growing HaCaT cells (Shanghai Yaji Biotechnology) and HSF cells (Guangzhou Genio Biotechnology) were seeded at a density of 2000 cells / well in 96-well culture plates. 100 μL of DMEM complete medium (containing 10% FBS + 1% antibiotics) was added to each well, and the plates were incubated at 37℃ for 24 h to form a monolayer of adherent cells.

[0070] 4. Sample processing

[0071] Sample preparation: After resuscitation and culture, the supernatant was discarded, and the cells were divided into a control group and an experimental group. For the control group, 100 μL of DMEM complete medium (containing 10% FBS + 1% penicillin antibody, v / v) was injected into each well of the culture plate. For the experimental group, 100 μL of DMEM complete medium containing 0.1% of the corresponding sample solution was injected into each well of the culture plate. After culturing for 24 h, cells from each group were collected, the medium was discarded, and the cells were washed twice with PBS. 1 mL of TRIzol lysis buffer was added to each well, and the cells were scraped and transferred to EP tubes for storage at -80℃ for later use.

[0072] 5. Protein expression detection (ELISA)

[0073] Claudin-1 assay: The Claudin-1 content in HaCaT cell lysate was determined according to the instructions of the human Claudin-1 ELISA kit (CSB-EL005490HU, Shanghai Huamei Biotechnology Co., Ltd.).

[0074] Type I collagen detection: The content of Col I in HSF cell lysate was determined according to the instructions of the human Col I ELISA kit (ml057630, Shanghai Enzyme-Link Biotechnology Co., Ltd.).

[0075] 6. Calculation of protein expression promotion rate

[0076]

[0077] The final protein expression promotion rate is shown in Table 1.

[0078] Table 1. Type I collagen promotion rate and tight junction protein promotion rate of each group of samples.

[0079]

[0080]

[0081] This test directly verifies the promoting effect of the composition on type I collagen and tight junction protein by detecting the promotion rates of type I collagen and tight junction protein. This test also evaluates the composition's effect on increasing skin tolerance before and promoting skin repair after cosmetic procedures by verifying its influence on tight junction protein in epidermal keratinocytes and collagen in the dermis.

[0082] As shown in Table 1, the data from Example 1 and Comparative Examples 1-5 indicate that the composition of Example 1, which uses five ingredients—oat β-glucan, oat kernel extract, holy basil leaf extract, hops extract, and Brazil nut seed extract—significantly improves the promotion rate of type I collagen and tight junction protein compared to the compositions of Comparative Examples 1-5. This suggests that the five ingredients have a synergistic effect: by upregulating Claudin-1 tight junction protein, they can strengthen barrier integrity; by promoting the synthesis of type I collagen (Collagen I), they can enhance dermal toughness, thereby improving barrier defense before surgery to resist damage, accelerating barrier reconstruction after surgery, reducing irritation sensitivity, and promoting wound healing.

[0083] Based on the data from Examples 1-7, it can be seen that when the weight ratio of oat β-glucan, oat kernel extract, holy basil leaf extract, hop extract, and Brazil nut seed extract is (1-1.2):(1.5-2):(1-2):(0.1-0.8):(0.8-1), the composition is more effective in promoting the production of type I collagen and tight junction proteins.

[0084] Test Example 2: Inhibition Test of Staphylococcus aureus and Propionibacterium acnes

[0085] 1. Preparation of experimental materials

[0086] Biological samples: Healthy zebrafish embryos 6-8 hours after fertilization (screening criteria: normal morphology and developed to the blastocyst stage).

[0087] Reagents:

[0088] Zebrafish embryo culture medium (containing methylene blue antibacterial agent), mixed bacterial culture of Staphylococcus aureus and Propionibacterium acnes (final concentration 1×10⁻⁶) 7 –5×10 7 CFU / mL, mixed at a 1:1 volume ratio), test samples of the compositions of Examples 1-7 and Comparative Examples 1-5 (mass concentration of 10%, dissolved in embryo culture medium).

[0089] Zebrafish embryo culture medium: Prepared by dissolving 2940mg anhydrous calcium chloride, 1233mg magnesium sulfate heptahydrate, 630mg sodium bicarbonate, 55mg potassium chloride, and 1g methylene blue in 10L of water. The pH value is 6.5-8.5. All chemicals are of analytical grade.

[0090] 2. Experimental Grouping

[0091] The cleaned, healthy zebrafish embryos, 6-8 hours post-fertilization, were grouped and processed as follows:

[0092] Control group: Zebrafish developed normally, and 200 μL of zebrafish embryo culture medium was added to each well during the culture process;

[0093] Control group: Zebrafish were injected with 10 μL of mixed bacterial solution in their tail fins before culture, and 200 μL of embryo culture medium was added to each well during culture.

[0094] Sample group: Zebrafish were injected with 10 μL of mixed bacterial solution in their caudal fins before culture, and 200 μL of embryo culture medium containing 10 wt% of the composition was added to each well during culture.

[0095] 3. Cultivation conditions

[0096] The blank group, control group and sample group were all placed in 24-well plates for culture, with at least 10 embryos in each group. After the healthy zebrafish embryos were treated according to the above grouping conditions, they were placed in a constant temperature incubator and cultured at 28℃ for 3 hours.

[0097] 4. Data Collection and Analysis

[0098] Fluorescence imaging: Ten zebrafish were randomly selected from each group and their dorsal region fluorescence images were captured under a fluorescence microscope. The data were analyzed and collected using the advanced image processing software ImageJ. The target area was precisely delineated using the rectangle tool, and the average fluorescence intensity of the selected area, i.e., the number of neutrophils in the zebrafish, was measured using the grayscale intensity method.

[0099] Neutrophil quantification: Data was analyzed and collected using the advanced image processing software ImageJ. The target area was precisely delineated using the rectangle tool, and the average fluorescence intensity of the selected area was measured using the grayscale intensity method, which is the number of neutrophils in zebrafish.

[0100] 5. Calculation of the inhibition rate of zebrafish neutrophils

[0101] See Table 2 for specific data.

[0102] Table 2. Inhibition rate data of zebrafish neutrophils in each group of samples.

[0103]

[0104]

[0105] The above test established a zebrafish inflammation model induced by Staphylococcus aureus and Propionibacterium acnes. The test sample was administered in water to induce an immune response in the zebrafish, causing neutrophils to migrate to and aggregate in the skin epidermis. The inhibition rate of neutrophils in the zebrafish was then measured, providing a direct indication of neutrophil aggregation in the zebrafish skin, thus determining whether the sample group could inhibit neutrophil aggregation induced by Propionibacterium acnes in zebrafish. A higher neutrophil inhibition rate indicates a greater ability of the composition to inhibit inflammation induced by Staphylococcus aureus and Propionibacterium acnes, demonstrating its anti-inflammatory and acne-preventing effects.

[0106] As shown in Table 2, and combining the data from Example 1 and Comparative Examples 1-5, the combination of the five components in Example 1—oat β-glucan, oat kernel extract, holy basil leaf extract, hops extract, and Brazil nut seed extract—significantly improved the inhibition rate of zebrafish neutrophils compared to the combinations in Comparative Examples 1-5. This indicates that the five components—oat β-glucan, oat kernel extract, holy basil leaf extract, hops extract, and Brazil nut seed extract—can synergistically inhibit the aggregation of zebrafish neutrophils, thereby inhibiting the production of inflammatory factors and achieving the effects of suppressing inflammation and preventing acne.

[0107] Based on the data from Examples 1-7, it can be seen that when the weight ratio of oat β-glucan, oat kernel extract, holy basil leaf extract, hop extract, and Brazil nut seed extract is (1-1.2):(1.5-2):(1-2):(0.1-0.8):(0.8-1), the composition has a better anti-inflammatory effect.

[0108] Application Example 1-7 and Comparative Application Example 1-5

[0109] The compositions of Examples 1-7 and Comparative Examples 1-5 were added to the serum at a concentration of 3 wt% to obtain the serums of Application Examples 1-7 and Comparative Application Examples 1-5. The formulations are shown in Table 3.

[0110] The preparation methods of the serums used in Application Examples 1-7 and Comparative Application Examples 1-5 include the following steps:

[0111] S1. Mix the humectant, thickener and 1 / 2 volume of deionized water, and homogenize at 80°C to obtain a mixture;

[0112] S2. When the temperature of the mixture in S1 drops to 60°C, add the preservative. When the temperature of the system drops to 40°C, add all the components in the composition and the remaining deionized water, stir evenly, and finally add the pH adjuster to adjust the pH to 6 to obtain the essence.

[0113] Table 3. Serum formulations for Application Examples 1-7 and Comparative Application Examples 1-5.

[0114]

[0115] Comparative Application Example 6

[0116] Compared to Application Example 6, the serum does not contain a non-invasive medical aesthetic repair and enhancement composition. Instead, it uses an equal amount of deionized water instead of the composition, and the preparation method is the same as in Application Example 1.

[0117] Test Example 3: Serum Efficacy Test

[0118] The experiment involved selecting subjects aged 20-50 years for a post-laser skin rejuvenation repair and soothing experiment, according to the "Cosmetic Safety Technical Specifications" (2015). Thirteen groups of four subjects were randomly assigned. All subjects understood the experimental requirements and agreed to undergo laser skin rejuvenation on their faces and complete the test. The M22 laser skin rejuvenation device was selected, and the hospital technician adjusted the intensity according to the subject's specific facial condition and different areas.

[0119] This test was divided into two phases. The first phase was before the photofacial rejuvenation treatment:

[0120] Subjects had their baseline facial physiological data measured 20 days prior to the procedure. Afterward, subjects applied the sample (the serum from Application Examples 1-7 and Control Application Examples 1-6) to their entire face twice daily, morning and evening, until the day of the phototherapy treatment, at which time facial physiological data were collected. Transdermal water loss (TEWL) and the percentage of red patches on the face were also collected before and after the procedure.

[0121] The second stage is post-photorejuvenation: After the photorejuvenation, the subjects' facial physiological data were measured. Subsequently, the sample was applied to the entire face twice daily, morning and evening. Facial physiological data was collected again after 20 days. Pre-treatment facial physiological data included: transepidermal water loss (TEWL) and the percentage of red area pixels on the face.

[0122] The methods for collecting facial physiological data are as follows:

[0123] Before and after the photofacial rejuvenation treatment, subjects sat quietly for 30 minutes in an air-conditioned room with a temperature of 21±1℃ and a humidity of 50±10%. A skin moisture loss testing probe was used. The CM 825 was used to test the transepidermal water loss (TEWL) value of the cheekbone. Since IPL skin rejuvenation is a non-invasive cosmetic procedure, post-procedure redness is not visually apparent. Therefore, a VISA-CR instrument was used to photograph the red area of ​​the subject's face, analyzing the proportion of red area pixels within the total effective facial area pixels to quantify the degree of deep skin redness. Post-procedure, a Colorimeter CL400 skin color testing probe was used to quantify the L* value of the subject's cheekbone.

[0124] The calculation formula is as follows:

[0125] Preoperative TEWL value improvement rate (%) = |(preoperative TEWL value) 使用后 -Preoperative Tewling Lung Value 使用前 Preoperative TEWL value 使用前 ×100%;

[0126] Postoperative TEWL value improvement rate (%) = |(Postoperative TEWL value) 使用后 -Postoperative TEEWL value 使用前 Postoperative TEWL value 使用前 ×100%;

[0127] Preoperative red zone pixel percentage improvement rate (%) = |(Preoperative red zone pixel percentage improvement rate) 使用后 - Improvement rate of preoperative red zone pixel ratio 使用前 Preoperative red zone pixel ratio improvement rate 使用前 ×100%;

[0128] Postoperative red area pixel percentage improvement rate (%) = |(Postoperative red area pixel percentage improvement rate) 使用后 - Improvement rate of red area pixel ratio after surgery 使用前 Postoperative red area pixel ratio improvement rate 使用前 ×100%;

[0129] Postoperative improvement in cheekbone luster L* value (%) = |(Postoperative cheekbone luster L* value) 使用后 -Postoperative cheekbone luster L* value 使用前 Postoperative cheekbone luster L* value 使用前 ×100%; the calculation results are shown in Table 4.

[0130] Table 4. Results of human trials for each group of serums.

[0131]

[0132] The ability of the composite material to strengthen the preoperative barrier in cosmetic procedures is represented by the improvement rate of preoperative TEWL value and the improvement rate of preoperative red area pixel ratio. The ability to repair after cosmetic procedures is represented by the improvement of postoperative TEWL value and the improvement rate of postoperative red area pixel ratio. The postoperative enhancement and prevention of hyperpigmentation are represented by the improvement of postoperative zygomatic bone luster L* value. The better the improvement rate, the better the corresponding ability.

[0133] Depend on Figure 1 It can be seen that the facial redness of the serum in Application Example 1 was significantly improved compared with the serum in Application Example 6 when used for 20 days before and 20 days after the procedure. This shows that the composition of the present invention can achieve the effects of barrier repair, redness reduction and prevention of hyperpigmentation before and after medical aesthetic procedures.

[0134] As shown in Table 4, Application Example 1 and Comparative Application Examples 1-5 exhibit significantly improved barrier repair, redness reduction, prevention of hyperpigmentation, and post-operative enhancement effects compared to Comparative Application Examples 1-5, which lack one of these components. This indicates that the five ingredients synergistically strengthen the skin barrier, inhibit inflammation, prevent pigmentation, reduce the risk of post-operative acne, and promote skin regeneration. This may be because oat β-glucan can form a protective film on the skin surface and promote the expression of related barrier factors to jointly reduce the skin's susceptibility to external stimuli. Oat kernel extract inhibits pro-inflammatory factors such as IL-6 and TNF-α by blocking the NF-κB signaling pathway. The ingredients are released; holy basil leaf extract blocks the melanin synthesis pathway by inhibiting tyrosinase activity and reducing dopaquinone intermediates, while simultaneously clearing ROS to protect dermal fibroblasts and reduce the incidence of postoperative pigmentation; Brazil nut seed extract activates the PPAR-γ pathway to promote lamellar body secretion, accelerates epidermal lipid metabolism, and avoids compensatory pigmentation caused by barrier defects. Simultaneously, selenoproteins in Brazil nut seed extract enhance desmoglein stability, promote keratinocyte differentiation and maturation, and accelerate the repair of damaged cells; hops extract reshapes the skin surface flora balance, reduces the proliferation of pathogenic bacteria such as Staphylococcus aureus, prevents acne breakouts caused by skin flora imbalance, and reduces neurogenic inflammatory responses by regulating transient receptor potential (TRPV1), thus reducing postoperative erythema. In summary, the composition of this invention, through a multi-dimensional synergistic mechanism, strengthens the skin barrier and increases tolerance before surgery, and enhances postoperative repair and prevents pigmentation and acne, achieving a closed-loop management of the entire cycle of "preoperative defense - postoperative repair - effect maintenance" in non-invasive medical aesthetics.

[0135] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A non-invasive, aesthetically pleasing repair and enhancement composition, characterized in that, The product comprises the following components: oat beta-glucan, oat kernel extract, holy basil leaf extract, hops extract, and Brazil nut seed extract, wherein the weight ratio of oat beta-glucan, oat kernel extract, holy basil leaf extract, hops extract, and Brazil nut seed extract is (0.11-3):(0.1-2):(1-5):(0.1-1):(0.2-2); wherein the oat beta-glucan is manufactured by Beijing Dongfang Miaosen Biotechnology Co., Ltd., and its trade name is Miaokexiu; the oat kernel extract is manufactured by Symrise Fragrance & Flavor (Nantong) Co., Ltd., and its trade name is... Avena; the manufacturer of the holy basil leaf extract is Shaanxi Snott Biotechnology Co., Ltd.; the manufacturer of the hops extract is BASF, with the trade name Senseryn™; the manufacturer of the Brazil nut seed extract is Clariant Chemicals (China) Co., Ltd., with the trade name Ethience Protect.

2. The non-invasive medical aesthetic repair and enhancement composition as described in claim 1, characterized in that, The weight ratio of oat β-glucan, oat kernel extract, holy basil leaf extract, hop extract and Brazil nut seed extract is (0.5-2):(1-2):(1-3):(0.1-1.2):(0.5-1.2).

3. The non-invasive medical aesthetic repair and enhancement composition as described in claim 1, characterized in that, The weight ratio of oat β-glucan, oat kernel extract, holy basil leaf extract, hop extract and Brazil nut seed extract is (1-1.2):(1.5-2):(1-2):(0.1-0.8):(0.8-1).

4. The use of the non-invasive medical aesthetic repair and enhancement composition according to any one of claims 1-3 in the preparation of cosmetics.

5. The application of the non-invasive medical aesthetic repair and enhancement composition as described in claim 4 in the preparation of cosmetics, characterized in that, The cosmetic is a toner, lotion, cream, mask, serum, or spray, and the amount of the composition added is 1%-5% of the total weight of the cosmetic.

6. An essence, characterized in that, The ingredients comprise the following ingredients by weight percentage: 1%-5% of the non-invasive medical aesthetic repair and enhancement composition according to any one of claims 1-3, 0.05%-0.5% thickener, 3%-10% moisturizer, 0.5%-3% preservative and 0.01%-0.3% pH adjuster, with the balance being deionized water.

7. The essence as described in claim 6, characterized in that, The raw material is selected from at least one of (a)-(d): (a) The thickener comprises at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyl dimethyl taurate copolymer, ammonium acryloyl dimethyl taurate / VP copolymer and sclerotium gum; (b) The moisturizer comprises at least one of allantoin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, D-panthenol, sodium hyaluronate, 1,2-butanediol, glycerin, budding stalk polysaccharide and ceramide; (c) The pH adjuster includes at least one of arginine, tromethamine and disodium EDTA; (d) The preservative includes at least one of 1,3-propanediol, 1,2-hexanediol and p-hydroxyacetophenone.

8. The method for preparing the essence according to claim 7, characterized in that, Includes the following steps: S1. Mix the humectant, thickener and part of the deionized water, homogenize at 75-85℃, then add the preservative and stir evenly to obtain the mixture. S2. Once the temperature of the mixture in S1 drops to 35-45℃, add the components of the non-invasive medical aesthetic repair and enhancement composition and the remaining deionized water, stir evenly, and finally add a pH adjuster to adjust the pH to obtain the essence.

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