Composition for clearing away fire and moistening lung as well as preparation method and application of composition

Abstract

The invention provides a fire-clearing and lung-moistening composition as well as a preparation method and application thereof, and belongs to the technical field of fire-clearing and lung-moistening compositions. The composition for clearing fire and moistening lung is prepared from the following raw materials in parts by weight: 10-100 parts of honeysuckle, 8-80 parts of chrysanthemum, 5-50 parts of momordica grosvenori, 4-40 parts of pericarpium citri reticulatae, 1-10 parts of lophatherum gracile and 1-10 parts of mint. The composition is prepared from common medicinal and edible raw materials, is scientific in compatibility, has the effects of clearing away fire and moistening the lung, and is high in safety and rich in source. The composition disclosed by the invention has a remarkable cough-relieving and / or anti-inflammatory effect, and the raw materials in the composition supplement each other and cannot be absent, so that the cough-relieving and / or anti-inflammatory effect cannot be achieved due to the lack of any raw material.

Description

Technical Field

[0001] The present invention belongs to the technical field of compositions for clearing heat and moistening the lungs, and particularly relates to a composition for clearing heat and moistening the lungs, a preparation method thereof, and an application thereof. Background Art

[0002] Pulmonary heat cough is a syndrome mainly manifested by cough due to internal heat accumulation in the lungs and failure of the lung qi to disperse. It is more common in children and the elderly with low immunity. In the scope of traditional Chinese medicine, pulmonary heat cough belongs to the febrile disease theory. Respiratory system diseases such as respiratory tract infections, acute and chronic bronchitis attacks in modern medicine may all present this disease. The clinical manifestations mainly include repeated coughing, expectoration, shortness of breath, accompanied by symptoms such as dry mouth, sore throat, constipation, red urine, fever, and inflammation. At present, most coughs are treated with antibiotics and cough suppressants, which have a certain therapeutic effect, but cannot eradicate lung diseases, and have certain side effects and dependencies. Minimizing and even eliminating the damage of harmful substances to the lungs according to the characteristics of the lungs and improving the disease resistance and health level of the lungs are the technical problems that need to be solved at present. Summary of the Invention

[0003] In view of this, the purpose of the present invention is to provide a composition for clearing heat and moistening the lungs, a preparation method thereof, and an application thereof.

[0004] In order to achieve the above-mentioned invention purpose, the present invention provides the following technical solutions:

[0005] The present invention provides a composition for clearing heat and moistening the lungs, which is made from the following raw materials in parts by weight:

[0006] 10 - 100 parts of honeysuckle, 8 - 80 parts of chrysanthemum, 5 - 50 parts of monk fruit, 4 - 40 parts of dried tangerine peel, 1 - 10 parts of lophatherum gracile, and 1 - 10 parts of mint.

[0007] Preferably, the composition is made from the following raw materials in parts by weight:

[0008] 50 - 100 parts of honeysuckle, 40 - 80 parts of chrysanthemum, 20 - 50 parts of monk fruit, 18 - 40 parts of dried tangerine peel, 5 - 10 parts of lophatherum gracile, and 5 - 10 parts of mint.

[0009] Preferably, the composition is made from the following raw materials in parts by weight:

[0010] 10 parts of honeysuckle, 8 parts of chrysanthemum, 5 parts of monk fruit, 4 parts of dried tangerine peel, 1 part of lophatherum gracile, and 1 part of mint.

[0011] Preferably, the composition is made from the following raw materials in parts by weight:

[0012] 50 parts of honeysuckle, 40 parts of chrysanthemum, 20 parts of monk fruit, 18 parts of dried tangerine peel, 5 parts of lophatherum gracile, and 5 parts of mint.

[0013] Preferably, the composition is made from the following raw materials in parts by weight:

[0014] 100 parts of honeysuckle, 80 parts of chrysanthemum, 50 parts of monk fruit, 40 parts of dried tangerine peel, 10 parts of lophatherum gracile, and 10 parts of mint.

[0015] The present invention provides a preparation method of the above composition, comprising the following steps:

[0016] Mix the honeysuckle, chrysanthemum, monk fruit, dried tangerine peel, lophatherum gracile, and mint with water, soak, boil, and extract with slow fire to obtain a water extract, filter, collect the filtrate, and obtain the composition.

[0017] Preferably, the ratio of the total mass of the honeysuckle, chrysanthemum, monk fruit, dried tangerine peel, lophatherum gracile, and mint to the volume of water is 1 g:(5 - 15) mL.

[0018] Preferably, the soaking temperature is 20 - 30 °C, and the soaking time is 15 - 25 min;

[0019] The number of times of slow fire extraction is 1 - 3 times, and the extraction time for each time is 20 - 90 min;

[0020] After collecting the filtrate, it further includes the step of concentrating the filtrate.

[0021] The present invention provides an application of the above composition or preparation method in the preparation of products for clearing heat and moistening the lungs.

[0022] The present invention provides an application of the above composition or preparation method in the preparation of products for relieving cough and / or anti - inflammation.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] The present invention provides a composition for clearing heat and moistening the lungs, its preparation method and application. The composition of the present invention uses common raw materials of both medicine and food, with scientific compatibility, has the effect of clearing heat and moistening the lungs, and has high safety and rich sources. The composition of the present invention has a significant effect of relieving cough and / or anti - inflammation, and the raw materials in the composition complement each other and are indispensable. The absence of any one raw material will lead to the inability to achieve the cough - relieving and / or anti - inflammatory efficacy. Description of the Drawings

[0025] Figure 1 It is the comparison result of the cough latency of mice in each group after cough induction after treatment with different groups;

[0026] Figure 2 It is the comparison result of the number of coughs within 2 min of mice in each group after cough induction after treatment with different groups;

[0027] Figure 3The comparison results of the ear swelling degrees of mice in each group after applying xylene after treatment with different groups;

[0028] Figure 4 The comparison results of the ear swelling inhibition rates of mice in each group after applying xylene after treatment with different groups. Detailed implementation manners

[0029] The present invention provides a composition for clearing heat and moistening the lungs, which is made from the following raw materials in parts by weight:

[0030] 10 - 100 parts of honeysuckle, 8 - 80 parts of chrysanthemum, 5 - 50 parts of momordica grosvenori, 4 - 40 parts of dried tangerine peel, 1 - 10 parts of lophatherum gracile, and 1 - 10 parts of mint.

[0031] In the present invention, as a preferred implementation manner, the composition is made from the following raw materials in parts by weight: 50 - 100 parts of honeysuckle, 40 - 80 parts of chrysanthemum, 20 - 50 parts of momordica grosvenori, 18 - 40 parts of dried tangerine peel, 5 - 10 parts of lophatherum gracile, and 5 - 10 parts of mint. Further preferably, the composition is made from the following raw materials in parts by weight: 10 parts of honeysuckle, 8 parts of chrysanthemum, 5 parts of momordica grosvenori, 4 parts of dried tangerine peel, 1 part of lophatherum gracile, and 1 part of mint; the composition is made from the following raw materials in parts by weight: 50 parts of honeysuckle, 40 parts of chrysanthemum, 20 parts of momordica grosvenori, 18 parts of dried tangerine peel, 5 parts of lophatherum gracile, and 5 parts of mint; the composition is made from the following raw materials in parts by weight: 100 parts of honeysuckle, 80 parts of chrysanthemum, 50 parts of momordica grosvenori, 40 parts of dried tangerine peel, 10 parts of lophatherum gracile, and 10 parts of mint.

[0032] In the present invention, honeysuckle (scientific name: Lonicera japonica Thunb.) is a plant of the genus Lonicera in the family Caprifoliaceae. Honeysuckle is sweet, pungent, bitter, and cold in nature, and has the functions of clearing heat and detoxifying, dispersing wind - heat, relieving sore throat, antibacterial and anti - inflammatory, antiviral, and immunomodulating. It is a plant with both medicinal and edible properties. Chrysanthemum is the dried capitulum of the plant Chrysanthemum morifolium Ramat. of the genus Chrysanthemum in the family Asteraceae. It is pungent, sweet, and bitter in taste, and slightly cold in nature, and has the effects of dispersing wind - heat, suppressing hyperactive liver - yang, clearing the liver and improving eyesight, clearing heat and detoxifying, and antibacterial and anti - inflammatory. Momordica grosvenori belongs to raw materials with both medicinal and edible properties. It is a climbing herbaceous plant of the genus Momordica in the family Cucurbitaceae, and has the functions of clearing heat and moistening the lungs, relieving sore throat and promoting voice, promoting fluid production and quenching thirst, anti - inflammatory and antibacterial, and enhancing immunity. Dried tangerine peel is the dried mature pericarp of the plant Citrus reticulata Blanco and its cultivated varieties of the family Rutaceae. Dried tangerine peel is warm in nature, pungent and bitter in taste, and has the effects of regulating qi and strengthening the spleen, drying dampness and resolving phlegm, promoting qi circulation and activating blood circulation, removing phlegm and relieving cough, antioxidant, anti - inflammatory, antibacterial, and antiviral. Lophatherum gracile is a perennial herbaceous plant of the family Poaceae. It is sweet and light in taste, and has the effects of clearing away heat and relieving restlessness, clearing the heat of the lungs, clearing the heart fire, clearing the stomach fire, and antibacterial. Mint is pungent in taste and cool in nature, and has the effects of dispersing wind - heat, promoting eruption of measles through the pharynx, detoxifying and removing foulness, antibacterial and antiviral, and analgesic and anti - inflammatory.

[0033] The present invention provides a preparation method of the above - mentioned composition, including the following steps:

[0034] Mix the honeysuckle, chrysanthemum, momordica grosvenori, tangerine peel, lophatherum gracile, and mint with water, soak, boil, and extract over a slow fire to obtain a water extract. Filter it and collect the filtrate to obtain the composition.

[0035] In the present invention, mix the honeysuckle, chrysanthemum, momordica grosvenori, tangerine peel, lophatherum gracile, and mint with water and soak. The total mass ratio of the honeysuckle, chrysanthemum, momordica grosvenori, tangerine peel, lophatherum gracile, and mint to the volume of water is preferably 1 g:(5 - 15) mL, more preferably 1 g:(6 - 12) mL, and even more preferably 1 g:6 mL or 1 g:10 mL. The soaking temperature is preferably 20 - 30 °C, more preferably 22 - 28 °C, and even more preferably 25 °C. The soaking time is preferably 15 - 25 min, more preferably 16 - 22 min, and even more preferably 18, 19, or 20 min.

[0036] In the present invention, after soaking, boil and extract over a slow fire to obtain a water extract. In the present invention, start the slow - fire extraction immediately after boiling. The number of slow - fire extraction times is preferably 1 - 3 times, more preferably 2 times. The extraction time for each time is preferably 20 - 90 min, more preferably 25 - 80 min, and even more preferably 30, 40, 50, or 60 min.

[0037] In the present invention, after obtaining the water extract, filter it and collect the filtrate. After collecting the filtrate, it further includes concentrating the filtrate until it is concentrated to one - third of the original volume to obtain a concentrated solution, which is the composition for clearing heat and moistening the lungs. The solid content in the concentrated solution prepared in the present invention is ≥15%. By the above - mentioned water - extraction method, the composition for clearing heat and moistening the lungs prepared in the present invention has significant cough - relieving and anti - inflammatory effects, thus achieving the purpose of clearing heat and moistening the lungs.

[0038] In the present invention, the composition for clearing heat and moistening the lungs of the present invention can be directly brewed and drunk with warm water. Therefore, the composition for clearing heat and moistening the lungs of the present invention is convenient to consume.

[0039] The present invention provides an application of the above composition or preparation method in the preparation of products for clearing heat and moistening the lungs.

[0040] The present invention provides an application of the above composition or preparation method in the preparation of products for relieving cough and / or anti - inflammation.

[0041] In the present invention, the product can be a drug or a functional beverage. The product of the present invention can also include pharmaceutically acceptable excipients. In the above - mentioned products, the present invention can use the above composition as the sole active ingredient for relieving cough and / or anti - inflammation, or combine it with other active ingredients for relieving cough and / or anti - inflammation. The weight ratio of the composition in the product is 50 - 100%.

[0042] The technical solution provided by the present invention will be described in detail below in conjunction with embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0043] Example 1

[0044] A composition for clearing heat and moistening the lungs is made from the following raw materials by weight:

[0045] 10 g of honeysuckle, 8 g of chrysanthemum, 5 g of momordica grosvenori, 4 g of dried tangerine peel, 1 g of lophatherum gracile and 1 g of mint.

[0046] The preparation method of the composition for clearing heat and moistening the lungs is as follows:

[0047] Mix 10 g of honeysuckle, 8 g of chrysanthemum, 5 g of momordica grosvenori, 4 g of dried tangerine peel, 1 g of lophatherum gracile, 1 g of mint with 290 mL of water, soak at 25°C for 20 min, then boil, and immediately extract with slow fire for 30 min after boiling, filter, collect the first filtrate and the first residue, mix the first residue with 174 mL of water and boil, immediately extract with slow fire for 1 h after boiling, filter, collect the second filtrate, combine the first filtrate and the second filtrate to obtain an extract, concentrate the extract until it is concentrated to one-third of the original volume to obtain a concentrated solution, which is the composition for clearing heat and moistening the lungs.

[0048] Example 2

[0049] A composition for clearing heat and moistening the lungs is made from the following raw materials by weight:

[0050] 50 g of honeysuckle, 40 g of chrysanthemum, 20 g of momordica grosvenori, 18 g of dried tangerine peel, 5 g of lophatherum gracile and 5 g of mint.

[0051] The preparation method of the composition for clearing heat and moistening the lungs is as follows:

[0052] Mix 50 g of honeysuckle, 40 g of chrysanthemum, 20 g of momordica grosvenori, 18 g of dried tangerine peel, 5 g of lophatherum gracile, 5 g of mint with 1380 mL of water, soak at 25°C for 20 min, then boil, and immediately extract with slow fire for 30 min after boiling, filter, collect the first filtrate and the first residue, mix the first residue with 828 mL of water and boil, immediately extract with slow fire for 1 h after boiling, filter, collect the second filtrate, combine the first filtrate and the second filtrate to obtain an extract, concentrate the extract until it is concentrated to one-third of the original volume to obtain a concentrated solution, which is the composition for clearing heat and moistening the lungs.

[0053] Example 3

[0054] A composition for clearing heat and moistening the lungs is made from the following raw materials by weight:

[0055] 100 g of honeysuckle, 80 g of chrysanthemum, 50 g of momordica grosvenori, 40 g of dried tangerine peel, 10 g of lophatherum gracile and 10 g of mint.

[0056] The preparation method of the composition for clearing heat and moistening the lungs is as follows:

[0057] Mix 100 g of honeysuckle, 80 g of chrysanthemum, 50 g of momordica grosvenori, 40 g of dried tangerine peel, 10 g of lophatherum gracile, 10 g of mint with 2900 mL of water, soak at 25°C for 20 min, then boil, immediately extract with slow fire for 30 min after boiling, filter, collect the first filtrate and the first residue, mix the first residue with 1740 mL of water and boil, immediately extract with slow fire for 1 h after boiling, filter, collect the second filtrate, combine the first filtrate and the second filtrate to obtain the extract, concentrate the extract until it is concentrated to one-third of the original volume to obtain the concentrated liquid, which is the composition for clearing heat and moistening the lungs.

[0058] Example 4

[0059] A composition for clearing heat and moistening the lungs is made from the following raw materials by weight:

[0060] 10 g of honeysuckle, 8 g of chrysanthemum, 5 g of momordica grosvenori, 4 g of dried tangerine peel, 1 g of lophatherum gracile and 1 g of mint.

[0061] The preparation method of the composition for clearing heat and moistening the lungs is as follows:

[0062] Mix 10 g of honeysuckle, 8 g of chrysanthemum, 5 g of momordica grosvenori, 4 g of dried tangerine peel, 1 g of lophatherum gracile, 1 g of mint with 290 mL of water, soak at 30°C for 25 min, then boil, immediately extract with slow fire for 90 min after boiling, filter, collect the filtrate as the extract, concentrate the extract until it is concentrated to one-third of the original volume to obtain the concentrated liquid, which is the composition for clearing heat and moistening the lungs.

[0063] Example 5

[0064] A composition for clearing heat and moistening the lungs is made from the following raw materials by weight:

[0065] 10 g of honeysuckle, 8 g of chrysanthemum, 5 g of momordica grosvenori, 4 g of dried tangerine peel, 1 g of lophatherum gracile and 1 g of mint.

[0066] The preparation method of the composition for clearing heat and moistening the lungs is as follows:

[0067] Mix 10 g of honeysuckle, 8 g of chrysanthemum, 5 g of monk fruit, 4 g of dried tangerine peel, 1 g of lophatherum gracile, 1 g of mint with 290 mL of water, soak at 30 °C for 15 min, then boil, immediately extract with gentle heat for 30 min after boiling, filter, collect the first filtrate and the first residue, mix the first residue with 174 mL of water and boil, immediately extract with gentle heat for 1 h after boiling, filter, collect the second filtrate and the second residue, mix the second residue with 145 mL of water and boil, immediately extract with gentle heat for 1 h after boiling, filter, collect the third filtrate, combine the first filtrate, the second filtrate and the third filtrate to obtain an extract, concentrate the extract until it is concentrated to one-third of the original volume to obtain a concentrated solution, which is the composition for clearing heat and moistening the lungs.

[0068] Comparative Example 1

[0069] A composition is made from the following raw materials by weight:

[0070] 20 g of monk fruit, 18 g of dried tangerine peel, 5 g of lophatherum gracile and 5 g of mint.

[0071] The preparation method of the composition is as follows:

[0072] Mix 20 g of monk fruit, 18 g of dried tangerine peel, 5 g of lophatherum gracile, 5 g of mint with 480 mL of water, soak at 25 °C for 20 min, then boil, immediately extract with gentle heat for 30 min after boiling, filter, collect the first filtrate and the first residue, mix the first residue with 288 mL of water and boil, immediately extract with gentle heat for 1 h after boiling, filter, collect the second filtrate, combine the first filtrate and the second filtrate to obtain an extract, concentrate the extract until it is concentrated to one-third of the original volume to obtain a concentrated solution, which is the composition.

[0073] Comparative Example 2

[0074] A composition is made from the following raw materials by weight:

[0075] 50 g of honeysuckle, 18 g of dried tangerine peel, 5 g of lophatherum gracile and 5 g of mint.

[0076] The preparation method of the composition is as follows:

[0077] Mix 50 g of honeysuckle, 18 g of dried tangerine peel, 5 g of lophatherum gracile, 5 g of mint with 780 mL of water, soak at 25 °C for 20 min, then boil, immediately extract with gentle heat for 30 min after boiling, filter, collect the first filtrate and the first residue, mix the first residue with 468 mL of water and boil, immediately extract with gentle heat for 1 h after boiling, filter, collect the second filtrate, combine the first filtrate and the second filtrate to obtain an extract, concentrate the extract until it is concentrated to one-third of the original volume to obtain a concentrated solution, which is the composition.

[0078] Comparative Example 3

[0079] A composition is made from raw materials of the following weights:

[0080] 40 g of chrysanthemum, 18 g of dried tangerine peel, 5 g of lophatherum gracile, and 5 g of mint.

[0081] The preparation method of the composition is as follows:

[0082] Mix 40 g of chrysanthemum, 18 g of dried tangerine peel, 5 g of lophatherum gracile, 5 g of mint with 680 mL of water, soak at 25°C for 20 min, then boil, immediately extract with slow fire for 30 min after boiling, filter, collect the first filtrate and the first residue, mix the first residue with 408 mL of water and boil, immediately extract with slow fire for 1 h after boiling, filter, collect the second filtrate, combine the first filtrate and the second filtrate to obtain an extract, concentrate the extract until it is concentrated to one-third of the original volume to obtain a concentrated solution, which is the composition.

[0083] Comparative Example 4

[0084] A composition is made from raw materials of the following weights:

[0085] 50 g of honeysuckle, 40 g of chrysanthemum, 20 g of momordica grosvenori, and 5 g of mint.

[0086] The preparation method of the composition is as follows:

[0087] Mix 50 g of honeysuckle, 40 g of chrysanthemum, 20 g of momordica grosvenori, 5 g of mint with 1150 mL of water, soak at 25°C for 20 min, then boil, immediately extract with slow fire for 30 min after boiling, filter, collect the first filtrate and the first residue, mix the first residue with 690 mL of water and boil, immediately extract with slow fire for 1 h after boiling, filter, collect the second filtrate, combine the first filtrate and the second filtrate to obtain an extract, concentrate the extract until it is concentrated to one-third of the original volume to obtain a concentrated solution, which is the composition.

[0088] Comparative Example 5

[0089] A composition is made from raw materials of the following weights:

[0090] 50 g of honeysuckle, 40 g of chrysanthemum, 20 g of momordica grosvenori, and 18 g of dried tangerine peel.

[0091] The preparation method of the composition is as follows:

[0092] Mix 50 g of honeysuckle, 40 g of chrysanthemum, 20 g of momordica grosvenori, and 18 g of dried tangerine peel with 1280 mL of water, soak at 25°C for 20 min, then boil, and immediately extract with slow fire for 30 min after boiling, filter, collect the first filtrate and the first filter residue. Mix the first filter residue with 768 mL of water and boil, and immediately extract with slow fire for 1 h after boiling, filter, collect the second filtrate, combine the first filtrate and the second filtrate to obtain an extract, concentrate the extract until it is concentrated to one-third of the original volume to obtain a concentrated solution, which is the composition.

[0093] Test Example 1

[0094] 1 Test method

[0095] 1.1 Cough-relieving effect test

[0096] A mouse cough model was prepared by the method of inducing cough with concentrated ammonia water.

[0097] Take 90 healthy Kunming mice, each weighing 20 g ± 2 g, with half male and half female, and randomly divide them into 9 groups, with 10 mice in each group, namely the blank control group (distilled water), Example 1 group, Example 2 group, Example 3 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, Comparative Example 4 group, and Comparative Example 5 group. Among them, the blank control group was intragastrically administered distilled water at a dose of 0.5 mL per 20 g body weight; the Example 1 group, Example 2 group, Example 3 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, Comparative Example 4 group, and Comparative Example 5 group were each intragastrically administered the concentrated solution prepared in Examples 1-3 and Comparative Examples 1-5 at a dose of 0.5 mL per 20 g body weight once a day for 4 consecutive days. One hour after the last administration, the mice in each group were placed in a cough induction device to induce cough (one at a time), and 0.2 mL of concentrated ammonia water was sprayed into each mouse. Immediately after 20 s, the mice were taken out, and the cough latency of the mice (the time from the start of spraying to the first cough of the mice) was observed, and the number of coughs of the mice within 2 min was recorded. The typical cough action was: abdominal muscle contraction or chest constriction, while opening the mouth wide and making a cough sound. The data statistical methods used were DesignExpert 10 and EXCEL.

[0098] As Figure 1 and Figure 2 The results show that compared with the control group, the fire-clearing and lung-moistening compositions prepared in Examples 1-3 can significantly prolong the cough latency caused by stimulating mice with concentrated ammonia water (P < 0.01), and significantly reduce the number of coughs of mice within 2 min after cough induction (P < 0.01), indicating that the fire-clearing and lung-moistening composition prepared by the present invention has a significant cough-relieving effect. Compared with the control group, the compositions prepared in Comparative Examples 1-5 have no obvious changes in the cough latency caused by stimulating mice with concentrated ammonia water and the number of coughs of mice within 2 min after cough induction, and have almost no cough-relieving effect.

[0099] 1.2 Anti-inflammatory effect test

[0100] An ear inflammation model of mice was prepared by the xylene-induced inflammation method.

[0101] Ninety Kunming mice with a body weight of 20 g ± 2 g per mouse, half male and half female, were randomly divided into 9 groups, with 10 mice in each group, namely the blank control group (distilled water), Example 1 group, Example 2 group, Example 3 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, Comparative Example 4 group, and Comparative Example 5 group. Among them, the blank control group was intragastrically administered distilled water at a dose of 0.5 mL per 20 g body weight; the Example 1 group, Example 2 group, Example 3 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, Comparative Example 4 group, and Comparative Example 5 group were respectively intragastrically administered 0.5 mL of the concentrated solution prepared in Examples 1-3 and Comparative Examples 1-5 per 20 g body weight. Each group was administered 2 times a day, once in the morning and once in the afternoon, for 3 consecutive days. 1.5 h after the last administration, 0.2 mL of xylene was evenly applied to the outer side of the right ear of each mouse in each group, and the left ear was not applied. After 0.5 h, the thickness of both ears was measured with a micrometer. The difference in the thickness of the two ear pieces was the swelling degree, and the swelling inhibition rate (%) was calculated according to the following formula:

[0102] Swelling inhibition rate = (swelling degree of the control group - swelling degree of the administration group) / swelling degree of the control group × 100%.

[0103] The data statistical method used Design Expert 10 and EXCEL.

[0104] As Figure 3 shown, compared with the control group, the compositions for clearing heat and moistening the lungs prepared in Examples 1-3 could significantly reduce the swelling degree of the auricles of mice caused by xylene (P < 0.01); while compared with the control group, the compositions prepared in Comparative Examples 1-5 had no obvious change in the swelling degree of the auricles of mice caused by xylene, and hardly had the effect of reducing the swelling degree of the auricles of mice caused by xylene.

[0105] As Figure 4 described, the swelling inhibition rate of the compositions for clearing heat and moistening the lungs prepared in Examples 1-3 increased with the increase in the amount of raw materials used. Among them, the swelling inhibition rates of the compositions for clearing heat and moistening the lungs prepared in Examples 1-3 and the compositions prepared in Comparative Examples 1-5 for the swelling of the auricles of mice caused by xylene were 34.43%, 40.35%, 52.33%, 6.31%, 4.67%, 4.16%, 5.3%, and 3.53% respectively, that is, compared with Comparative Examples 1-5, the compositions for clearing heat and moistening the lungs prepared in Examples 1-3 could significantly inhibit the swelling of the auricles of mice caused by xylene. It shows that the composition for clearing heat and moistening the lungs prepared by the present invention has a significant anti-inflammatory effect.

[0106] The above are only the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and refinements can be made, and these improvements and refinements should also be regarded as the protection scope of the present invention.

Claims

1. A composition for clearing heat and moistening the lungs, characterized in that, It is made from the following raw materials in parts by weight: 10 - 100 parts of honeysuckle flower, 8 - 80 parts of chrysanthemum flower, 5 - 50 parts of monk fruit, 4 - 40 parts of dried tangerine peel, 1 - 10 parts of lophatherum gracile, and 1 - 10 parts of mint.

2. The composition according to claim 1, wherein It is made from the following raw materials in parts by weight: 50 - 100 parts of honeysuckle flower, 40 - 80 parts of chrysanthemum flower, 20 - 50 parts of monk fruit, 18 - 40 parts of dried tangerine peel, 5 - 10 parts of lophatherum gracile, and 5 - 10 parts of mint.

3. The composition according to claim 1, characterized in that, It is made from the following raw materials in parts by weight: 10 parts of honeysuckle flower, 8 parts of chrysanthemum flower, 5 parts of monk fruit, 4 parts of dried tangerine peel, 1 part of lophatherum gracile, and 1 part of mint.

4. The composition according to claim 1 or 2, characterized in that, It is made from the following raw materials in parts by weight: 50 parts of honeysuckle flower, 40 parts of chrysanthemum flower, 20 parts of monk fruit, 18 parts of dried tangerine peel, 5 parts of lophatherum gracile, and 5 parts of mint.

5. The composition according to claim 1 or 2, characterized in that, It is made from the following raw materials in parts by weight: 100 parts of honeysuckle flower, 80 parts of chrysanthemum flower, 50 parts of monk fruit, 40 parts of dried tangerine peel, 10 parts of lophatherum gracile, and 10 parts of mint.

6. A method for preparing the composition according to any one of claims 1 to 5, characterized in that, It includes the following steps: Mix the honeysuckle flower, chrysanthemum flower, monk fruit, dried tangerine peel, lophatherum gracile, and mint with water, soak, boil, and extract with slow fire to obtain a water extract, filter, and collect the filtrate to obtain the composition.

7. The preparation method according to claim 6, characterized in that, The mass ratio of the total of the honeysuckle flower, chrysanthemum flower, monk fruit, dried tangerine peel, lophatherum gracile, and mint to the volume of water is 1 g : (5 - 15) mL.

8. The preparation method according to claim 6, characterized in that, The temperature of the soaking is 20 - 30 °C, and the time of the soaking is 15 - 25 min; The number of times of extraction with slow fire is 1 - 3 times, and the time of each extraction is 20 - 90 min; After collecting the filtrate, it further includes the step of concentrating the filtrate.

9. Use of the composition according to any one of claims 1 - 5 or the preparation method according to any one of claims 6 - 8 in the preparation of a product for clearing heat and moistening the lungs.

10. Use of the composition according to any one of claims 1 - 5 or the preparation method according to any one of claims 6 - 8 in the preparation of a product for relieving cough and / or anti - inflammation.

Images