A composition, use and cosmetic having enhanced skin self-protection

Abstract

The application belongs to the field of daily chemicals, and discloses a composition with improved skin self-protection, which comprises the following components: 6-8 wt% of a phosphoric group modified lactoferrin solution; 1.2-1.5 wt% of an alginate oligosaccharide; 1-2 wt% of plant oil with skin barrier repair function; emulsifier; and the balance of water; the concentration of the phosphoric group modified lactoferrin in the phosphoric group modified lactoferrin solution is 1.2-2 wt%. The composition uses the phosphoric group modified lactoferrin to improve the repair effect of the alginate oligosaccharide and the functional plant oil on the skin, and the composition can not only relieve the dry skin function, but also improve the physical barrier and immune barrier functions of the skin, and improve the self-protection ability of the skin. Another purpose of the application is to provide the application of the composition.

Description

Technical Field

[0001] This invention relates to the field of cosmetics, and more specifically to a composition, application, and cosmetic that enhances the skin's self-protective ability. Background Technology

[0002] The skin is the largest organ in the human body, with four main barrier functions: microbial barrier, chemical barrier, physical barrier, and immune barrier. These interconnected elements form a complete barrier system and a powerful defense system. However, infants and children, whose skin is not yet fully developed, have skin structures and functions different from adults. Their skin has a thinner stratum corneum, a richer capillary network, less sebum secretion, softer intercellular lipids, and weaker resistance. If not properly cared for, it is prone to symptoms such as dryness, itching, and redness, and in severe cases, can lead to dermatitis, eczema, and other conditions.

[0003] Peony seed oil is a plant oil extracted from peony seeds, with an unsaturated fatty acid content as high as 92%. It is rich in linoleic acid and linolenic acid, which are the main components of cell membranes and intercellular lipids that build the skin barrier, participate in the formation of the stratum corneum barrier, reduce inflammation, and improve allergies.

[0004] Seaweed oligosaccharides are increasingly being used in cosmetics due to their natural, nutritious, non-toxic, and non-irritating properties. They offer benefits such as skin moisturizing, free radical scavenging, and UV protection. The molecular structure of seaweed oligosaccharides contains numerous polar groups such as hydroxyl and carboxyl groups, which can form hydrogen bonds with water molecules and cross-link to form a network structure, exhibiting excellent water absorption. Simultaneously, seaweed oligosaccharides possess excellent film-forming properties, forming a uniform film on the skin surface, reducing water evaporation, and allowing moisture to diffuse from the basal tissue to the stratum corneum, inducing further hydration of the stratum corneum and preserving the skin's own moisture, thus achieving a moisturizing effect.

[0005] In the prior art, the application of combinations of plant oils and seaweed oligosaccharides in skin care can be found in the following literature:

[0006] Patent application CN117243840A, entitled "A Fullerene Repairing and Moisturizing Cream and Its Preparation Method and Application", discloses that the cream contains trehalose, fucoidan, maltodextrin, meadowfoam seed oil, jojoba oil, etc.

[0007] Patent application CN117959235A, entitled "A Composition for Regulating Skin Microecological Balance and Its Application," discloses that the moisturizing agent is selected from any one or a combination of at least two of the following: polyols, hyaluronic acid, sodium hyaluronate, trehalose, ceramide, betaine, seaweed extract, and hydroxypropyl cyclodextrin; and the skin moisturizer is selected from any one or a combination of at least two of the following: meadowfoam seed oil, sunflower seed oil, mango seed ester, grape seed oil, sweet almond oil, shea butter, jojoba seed oil, avocado oil, squalane, caprylic / capric triglyceride, polydimethylsiloxane, isononyl isononanoate, cetyl ethylhexanoate, or hydrogenated polyisobutylene.

[0008] The combination of the aforementioned seaweed oligosaccharides and functional plant oils can enhance the skin's self-protective ability, but its enhancing effect is still insufficient to meet the expectations of this project. Therefore, this proposal is put forward. Summary of the Invention

[0009] One of the objectives of this invention is to provide a composition that enhances the skin's self-protection ability. This composition uses phosphate-modified lactoferrin to enhance the skin repair effect of seaweed oligosaccharides and functional plant oils. The composition of this invention can not only relieve skin dryness, but also enhance the skin's physical and immune barrier functions, thereby improving the skin's self-protection ability.

[0010] Another object of the present invention is to provide the application of the composition.

[0011] To achieve the above objectives, the present invention provides a composition that enhances the skin's self-protective ability, comprising the following components:

[0012] A phosphate-modified lactoferrin solution of 6–8 wt%;

[0013] Algal oligosaccharides: 1.2–1.5 wt%;

[0014] 1-2 wt% of plant oils with skin barrier repair function;

[0015] Emulsifier and the remainder water;

[0016] The concentration of phosphate-modified lactoferrin in the phosphate-modified lactoferrin solution is 1.2–2 wt%.

[0017] This invention uses a phosphate-modified lactoferrin solution as a penetration enhancer for algal oligosaccharides and plant oils, which can improve the moisturizing effect of algal oligosaccharides and plant oils on the skin.

[0018] Meanwhile, lactoferrin itself has multiple effects on the skin, particularly excelling in improving skin barrier function and anti-inflammation. Lactoferrin can improve skin barrier function by reducing irritation through inhibiting the secretion of inflammatory factors. It can bind to bacterial walls, oxidizing bacteria by forming peroxides, increasing bacterial cell membrane permeability, and causing bacterial lipopolysaccharides to leak out of the outer membrane, thus exerting a bactericidal effect. Furthermore, lactoferrin can inhibit pathogenic fungi that cause skin diseases, such as Trichophyton mentagrophytes and Candida albicans, which are associated with various skin conditions.

[0019] Furthermore, this invention uses phosphate groups to modify lactoferrin, which can further enhance lactoferrin's ability to repair skin barrier function and improve skin's self-protection capabilities.

[0020] Based on the above functions, the composition of the present invention can also enhance the physical and immune barrier functions of the skin, thereby improving the skin's self-protection ability.

[0021] In the above-mentioned composition that enhances the skin's self-protection ability, the algal oligosaccharide is one or more of the following: seaweed oligosaccharide, fucoidan oligosaccharide, agar oligosaccharide, brown algae oligosaccharide, and carrageenan oligosaccharide.

[0022] The plant oil with skin barrier repair function is one or more of peony seed oil, sunflower seed oil, and camellia seed oil.

[0023] In the above-mentioned composition that enhances the skin's self-protection ability, the emulsifier is one or more combinations of polyglycerol-3-methylglucose distearate, cetearyl oleate, sorbitan oleate, and polyglycerol-3-distearate;

[0024] The amount of the emulsifier used is 0.05 to 0.2 wt%.

[0025] In the above-mentioned composition that enhances the skin's self-protection ability, the method for preparing the phosphate-modified lactoferrin solution is as follows:

[0026] In the presence of a thickener, lactoferrin and a modifier with a phosphate polar group are mixed and reacted to obtain phosphate-modified lactoferrin.

[0027] The modifier is one or more of lecithin, hydrogenated lecithin, and glycerophosphate choline.

[0028] In the above-mentioned composition for enhancing the skin's self-protection ability, the weight ratio of lactoferrin to the modifier is 1:0.2 to 1.

[0029] In the above-mentioned composition that enhances the skin's self-protection ability, the thickener is xanthan gum or guar gum.

[0030] In the above-mentioned composition that enhances the skin's self-protection ability, the ratio of the thickener to lactoferrin is 0.1 to 0.3:1.

[0031] In the above-mentioned composition that enhances the skin's self-protection ability, the method for preparing the phosphate-modified lactoferrin solution includes the following steps:

[0032] Step 1: Add the modifier and thickener to the water to dissolve them;

[0033] Step 2: Add lactoferrin to the solution from Step 1 at 30–50°C, stir and keep warm for 3–5 hours; then homogenize and filter, the resulting filtrate contains the phosphate-modified lactoferrin.

[0034] The weight ratio of the modifier to water is 0.2–1:50–85.

[0035] In addition, the present invention also discloses the use of the compositions described above in the preparation of cosmetics.

[0036] Finally, a cosmetic product containing any of the compositions described above was also disclosed.

[0037] Beneficial effects

[0038] This invention uses a phosphate-modified lactoferrin solution as a permeation enhancer for algal oligosaccharides and plant oils, which can improve the moisturizing effect of algal oligosaccharides and plant oils on the skin, enhance the skin's physical and immune barrier functions, and improve the skin's self-protection ability. Attached Figure Description

[0039] Figure 1 These are the results of stability tests on each sample at 45℃ for 4 weeks. Detailed Implementation

[0040] The present invention will be further described below with reference to embodiments, but this does not constitute any limitation on the present invention. Any limited modifications made within the scope of the claims of the present invention are still within the scope of the claims of the present invention.

[0041] To illustrate the technical content of the present invention in detail, the following description is provided in conjunction with the embodiments.

[0042] Part 1: Preparation and Functional Identification of Phosphate-Modified Lactoferrin Solutions

[0043] Examples and Comparative Examples

[0044] Step 1: Preparation of the modifier solution: Slowly dissolve xanthan gum in deionized water, mix and stir at room temperature, add a certain amount of modifier, heat to 80℃ to fully dissolve the modifier and xanthan gum, homogenize at 5000r / min for 2min to disperse evenly, and cool to 40℃.

[0045] Step 2: Add the lyophilized lactoferrin powder to the solution in Step 1 above, stir at 40°C for 1 hour to fully dissolve the lactoferrin and modifier, and keep warm for 4 hours to allow the components to interact.

[0046] A primary emulsion was prepared by pre-emulsification at 8000 rpm for 2 minutes using an emulsifier, followed by secondary homogenization using a high-pressure homogenizer at 40 MPa. After homogenization, the solution was filtered, and the filtrate was a solution containing phosphate-modified lactoferrin.

[0047] The formula can be found in Table 1.

[0048] Table 1 Formula Table (Unit: g)

[0049]

[0050]

[0051] Performance testing

[0052] High and low temperature stability testing: Referencing the "Technical Guidelines for Stability Testing and Evaluation of Cosmetics" (China National Institutes for Food and Drug Control, July 2024), the stability of Examples 1-9 and Comparative Example 1 was examined at 5°C, room temperature, and 45°C for 4 weeks. The results are shown in Table 2 below. Figure 1 ;

[0053] Table 2 Stability Test Results

[0054]

[0055] Note: √ indicates no abnormality.

[0056] As can be seen from the table, in Comparative Example 1 without modification, slight stratification occurred at 45°C for both 2 and 4 weeks. This is related to the inherent stability of the lactoferrin solution. The stratification phenomenon improved after adding the modifier and changing the amount of thickener. A comparison of Examples 1-3 and Examples 4-6 shows that hydrogenated lecithin is more stable than lecithin, which is related to the improved stability and emulsifying properties of lecithin after hydrogenation. Examples 7-9 remained stable, indicating that glycerophosphate choline helps maintain the stability of the lactoferrin solution.

[0057] Permeability test

[0058] The permeability test was conducted in accordance with GB / T 27818-2011 "In vitro test method for skin absorption of chemicals". Based on the pig skin-Franz diffusion cell system, the test sample was applied to the surface of isolated skin, and then the cumulative permeation, recovery rate and cumulative permeability were calculated to evaluate the transdermal permeability of the test sample.

[0059] The test results are shown in Table 3 below;

[0060] Table 3. Permeability Test Results

[0061]

[0062] The results show that the permeability of Examples 2, 5, and 8 was improved, and this improvement was related to the amount of modifier. The results varied among different modifiers. Overall, with the same amount of modifier added, glycerophosphocholine > hydrogenated lecithin > lecithin. The permeability of hydrogenated lecithin and lecithin is related to their ability to form liposomes, which helps lactoferrin permeate. Glycerophosphocholine lacks a fatty acid structure and does not form liposomes, but it also has a significant permeation-enhancing effect. This may be related to its small molecular weight; for the same mass, it has more phosphocholine polar groups, which have a greater impact on the secondary structure of lactoferrin.

[0063] Part Two: Preparation and Functional Verification of the Composition

[0064] The preparation method of the combination of the present invention is as follows:

[0065] Step 1: Weigh and mix the ingredients according to the formula in Table 4;

[0066] Step 2: Add the algae oligosaccharides, vegetable oils and emulsifiers to water, stir and heat to 80±2℃ to dissolve and mix evenly, then homogenize using a homogenizer at 8000r / min for 2 minutes to disperse evenly, and keep warm for 15 minutes;

[0067] Step 3: Turn on the cooling water, stir and cool down to 40±2℃, add lactoferrin solution and stir evenly; cool down to room temperature to obtain the composition.

[0068] The formula is shown in Table 4;

[0069] Table 4 Formula Table (Unit: g)

[0070]

[0071] Performance testing

[0072] In vitro anti-inflammatory experiment of the composition

[0073] Experimental Principle: Referencing "T / GDCQMA 005-2024 Cosmetic Soothing Efficacy Test—In Vitro Skin Keratinocyte Inflammatory Factor Test Method". Under the stimulation of external environmental factors or internal physiological factors, skin keratinocytes secrete TNF-α, thereby promoting cellular oxidative stress and inflammatory responses. IL-6, as a typical pro-inflammatory factor, mainly functions to stimulate the growth and differentiation of T lymphocytes and B lymphocytes, induce cytotoxic T lymphocytes to produce acute-phase reaction proteins, and promote inflammatory responses. Therefore, IL-6 can directly reflect the body's inflammatory status. A cellular inflammation model was established using TNF-α-induced immortalized human keratinocytes. After the cellular inflammation model was treated with test samples, the IL-6 content secreted by the cells was detected using enzyme-linked immunosorbent assay (ELISA). The anti-inflammatory ability of the test samples was judged based on changes in IL-6 expression.

[0074] The test results are shown in Table 5;

[0075] Table 5 Results of inflammatory factor testing

[0076]

[0077]

[0078] Skin moisturizing test method for the composition: Thirty healthy individuals aged 20-50 years were selected. A 2% sodium lauryl sulfate (SLS) aqueous solution was applied to the skin of their arms. After 24 hours, the patch was removed, and the participants sat still for 30 minutes before testing the initial skin values ​​T0 (transepidermal water loss rate and skin erythema index). After the test, the control group was left untreated; the sample groups were treated with the products of the examples and comparative examples, respectively, twice daily, morning and evening. After 24 hours, a second data measurement T1 (transepidermal water loss rate and skin erythema index) was performed; after 72 hours, a third data measurement T3 (transepidermal water loss rate and skin erythema index) was performed.

[0079] The relevant test results are shown in Table 6;

[0080] Table 6. Results of Moisturizing Performance Test

[0081]

[0082] Results analysis:

[0083] 1. As can be seen from Table 5, the modified lactoferrin solution has a better ability to inhibit inflammatory factors; as can be seen from both the examples and Comparative Example 3, the solution containing lactoferrin has a certain ability to inhibit inflammatory factors; when it is used in combination with algal oligosaccharides and plant oils to form an emulsion, its ability to inhibit inflammatory factors is significantly improved.

[0084] 2. As can be seen from Table 6, the emulsion of the present invention can significantly improve the skin moisturizing effect. As can be seen from the data of Comparative Examples 3 and 4, as long as lactoferrin is used, it has a certain moisturizing performance; when it is used in combination with algae oligosaccharides and plant oils to form an emulsion, its moisturizing performance is even better.

[0085] The embodiments presented herein are merely selected implementations based on combinations of all possible embodiments. The appended claims should not be limited to the embodiments described herein. Some numerical ranges used in the claims include sub-ranges within them, and variations within these ranges should also be covered by the appended claims.

Claims

1. A composition that enhances the skin's self-protective ability, characterized in that, It includes the following components: 6–8 wt% phosphate-modified lactoferrin solution Algal oligosaccharides: 1.2–1.5 wt%; Plant oils with skin barrier repair function (1-2 wt%) Emulsifier and the remainder water; The concentration of phosphate-modified lactoferrin in the phosphate-modified lactoferrin solution is 1.2–2 wt%; the algal oligosaccharide is one or more combinations of seaweed oligosaccharide, fucoidan oligosaccharide, agar oligosaccharide, brown algae oligosaccharide, and carrageenan oligosaccharide. The plant oil with skin barrier repair function is one or more of the following: peony seed oil, sunflower seed oil, and camellia seed oil. The method for preparing the phosphate-modified lactoferrin solution is as follows: In the presence of a thickener, lactoferrin and a modifier with a phosphate polar group are mixed and reacted to obtain phosphate-modified lactoferrin. The modifier is glycerophosphate choline.

2. The composition for enhancing skin's self-protective ability according to claim 1, characterized in that, The emulsifier is one or a combination of polyglycerol-3-methylglucose distearate, cetearyl oleate, sorbitan oleate, and polyglycerol-3-distearate; The amount of the emulsifier used is 0.05 to 0.2 wt%.

3. The composition for enhancing skin's self-protective ability according to claim 1, characterized in that, The weight ratio of lactoferrin to the modifier is 1:0.2 to 1.

4. The composition for enhancing skin's self-protective ability according to claim 1, characterized in that, The thickener is xanthan gum or guar gum.

5. The composition for enhancing skin's self-protective ability according to claim 4, characterized in that, The ratio of the thickener to lactoferrin is 0.1 to 0.3:

1.

6. The composition for enhancing skin's self-protective ability according to claim 1, characterized in that, The preparation method of the phosphate-modified lactoferrin solution includes the following steps: Step 1: Add the modifier and thickener to the water to dissolve them; Step 2: Add lactoferrin to the solution from Step 1 at 30–50°C, stir and keep warm for 3–5 hours; then homogenize and filter, the resulting filtrate contains the phosphate-modified lactoferrin. The weight ratio of the modifier to water is 0.2–1:50–85.

7. Use of the composition according to any one of claims 1 to 6 in the preparation of cosmetics.

8. A cosmetic product, characterized in that, Contains the composition as described in any one of claims 1 to 6.

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