A composition for post-medical aesthetic repair and soothing, its preparation method and application

Abstract

This invention discloses a post-medical aesthetic repair and soothing composition, its preparation method, and its application, relating to the field of cosmetic technology. The invention provides a repair and soothing composition comprising the following components in parts by weight: 0.1-1 parts citrus fruit extract, 0.1-0.5 parts date palm fruit extract, 0.05-0.5 parts gentian root extract, and 0.1-1 parts liposomes; wherein the liposomes comprise dipotassium glycyrrhizate and phytol. The repair and soothing composition provided by this invention achieves rapid repair and long-term stabilization through a multi-dimensional synergistic mechanism. The above-mentioned composition of this invention synergistically regulates skin pH, instantly blocks the transmission of neurogenic pain signals mediated by TRPV1 receptors, synergistically rebuilds the physical and microecological barriers, promotes vasoconstriction, and activates skin TRPM cold receptors, achieving the effect of a "cold compress."

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and in particular to a composition for post-medical aesthetic repair and soothing, its preparation method, and its application. Background Technology

[0002] In the current field of medical aesthetics, phototherapy (including intense pulsed light, laser, radiofrequency, etc.) and injectable techniques (such as botulinum toxin and hyaluronic acid fillers) have become routine treatments for improving skin laxity, pigmentation, and acne. Although these techniques can achieve a regenerative repair mechanism through controlled skin damage, significant postoperative complications still exist in clinical applications: ① Thermal damage from phototherapy can disrupt the continuity of the stratum corneum, leading to an abnormally high transepidermal water loss rate (TWEL), causing persistent skin dryness, sensitivity, and erythema; ② Injection procedures can easily cause mechanical damage to the vascular endothelium, inducing local tissue ischemic lesions, forming secondary damage such as vascular embolism and subcutaneous induration, while the puncture channel creates an invasion path for pathogenic microorganisms to colonize.

[0003] To address the above issues, existing repair techniques mainly employ physical barrier repair agents such as hyaluronic acid moisturizing gel and collagen dressings, supplemented with anti-inflammatory components for symptom control. However, experimental verification has revealed the following key defects in this technology system: (1) Lack of acid-base balance regulation mechanism: Postoperative wound exudate causes abnormal fluctuations in the pH value of the skin surface, directly affecting the endogenous biosynthesis pathway of ceramides, resulting in a delay in the barrier repair process; (2) Insufficient pain modulation efficacy: Existing components lack the ability to immediately block the transmission of neurogenic pain signals mediated by TRPV1 receptors; (3) Lack of microbial barrier reconstruction: No synergistic regulation mechanism with symbiotic flora such as Staphylococcus epidermidis and Propionibacterium acnes has been established, increasing the risk of colonization of pathogenic bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa after surgery; (4) Outdated cryotherapy technology: Current ice pack cold compresses have problems such as uncontrollable temperature gradient and inaccurate action sites.

[0004] Based on this, a composition for post-medical aesthetic repair and soothing that achieves rapid repair and long-term stabilization through a multi-dimensional synergistic mechanism has broad application prospects. Summary of the Invention

[0005] Based on this, the purpose of this invention is to overcome the shortcomings of the prior art and provide a post-medical aesthetic repair and soothing composition, its preparation method, and its application. The repair and soothing composition provided by this invention can immediately block the transmission of neurogenic pain signals mediated by TRPV1 receptors, promote vasoconstriction, and activate skin TRPM cold receptors to achieve the effect of "cold compress." At the same time, it can dynamically regulate skin pH, synergistically rebuild the physical barrier and microecological barrier, and achieve rapid repair and long-term stability. The post-medical aesthetic repair and soothing composition provided by this invention can achieve rapid repair and long-term stability through a multi-dimensional synergistic mechanism.

[0006] To achieve the above objectives, the technical solution adopted by the present invention is as follows: a repairing and soothing composition comprising the following components in parts by weight: 0.1-1 parts of citrus fruit extract, 0.1-0.5 parts of date palm fruit extract, 0.05-0.5 parts of gentian root extract, and 0.1-1 parts of liposomes; wherein the liposomes comprise dipotassium glycyrrhizate and phytol.

[0007] The repair and soothing composition provided by this invention achieves rapid repair and long-term stabilization through a multi-dimensional synergistic mechanism, specifically as follows: Rapid repair is reflected in the fact that the post-medical aesthetic repair and soothing composition provided by this invention can alleviate postoperative pain, burning sensation, redness, and swelling. Long-term stabilization is reflected in the fact that the post-medical aesthetic repair and soothing composition provided by this invention can rebuild the physical barrier and microecological barrier, maintain skin homeostasis in the long term, and cover the entire postoperative repair cycle. The above-mentioned composition of this invention can synergistically block the transmission of neurogenic pain signals mediated by TRPV1 receptors, promote vasoconstriction, and activate skin TRPM cold receptors, achieving the effect of "cold compress"; at the same time, it can dynamically regulate skin pH, synergistically rebuild the physical barrier and microecological barrier, and achieve rapid repair and long-term stabilization. The inventors discovered that the liposomes of this invention include dipotassium glycyrrhizate and phytol. Dipotassium glycyrrhizate, as a water-soluble component, is encapsulated by the hydrophilic core of the liposome, while phytol, as a lipid-soluble component, is embedded in the lipid bilayer, forming a "hydrophilic-hydrophobic" biphasic carrier. The specific liposome structure of this invention solves the problem of low water solubility of phytol, while protecting dipotassium glycyrrhizate and phytol from photothermal degradation. The specific dipotassium glycyrrhizate-phytol liposomes of this invention can better achieve rapid repair and long-term stabilization.

[0008] Preferably, the repairing and soothing composition comprises the following components in parts by weight: 0.3-0.8 parts of citrus fruit extract, 0.2-0.3 parts of date palm fruit extract, 0.1-0.3 parts of gentian root extract, and 0.3-0.5 parts of liposomes.

[0009] During actual experiments, the inventors discovered that when the components in the above composition are within a specific weight range, they can better achieve rapid repair and long-term stabilization. The post-medical aesthetic repair and soothing composition provided by this invention can instantly block the transmission of neurogenic pain signals mediated by TRPV1 receptors, promote vasoconstriction, and activate skin TRPM cold receptors, achieving the effect of "cold compress." At the same time, it can dynamically regulate skin pH, synergistically rebuild the physical barrier and microecological barrier, and achieve rapid repair and long-term stabilization.

[0010] Preferably, the liposomes comprise 30-40% by weight of the total weight of the repairing and soothing composition.

[0011] During actual experiments, the inventors discovered that when the weight percentage of the liposomes is within the above range, it can better regulate skin pH, synergistically rebuild the physical barrier and the microecological barrier, and better achieve long-term stability.

[0012] Preferably, the weight ratio of the citrus fruit extract, date palm fruit extract, and gentian root extract is citrus fruit extract: date palm fruit extract: gentian root extract = (2-3): 1: 1.

[0013] During actual experiments, the inventors discovered that when the weight ratio of citrus fruit extract, date palm fruit extract, and gentian root extract was in the specific proportions mentioned above, the repairing and soothing composition was more effective in relieving postoperative pain, burning sensation, redness, and swelling.

[0014] The liposomes prepared according to the present invention can be prepared by either thin-film dispersion or reverse evaporation.

[0015] Preferably, the method for preparing the liposomes includes the following steps:

[0016] (1) Soybean lecithin, cholesterol, phytol, and 1,2-dioleoyl-3-trimethylammonium chloride alanine (DOTAP) were mixed and dissolved in solution A, and the lipid membrane was obtained by rotary evaporation; solution A was a mixed solution of chloroform and ethanol.

[0017] (2) Add solution B to the lipid membrane in step (1), and hydrate and homogenize by sonication to obtain the liposomes; the solution B is a phosphate buffer containing dipotassium glycyrrhizate.

[0018] This invention provides a method for preparing liposomes using a thin-film dispersion method. When liposomes prepared using this method are used in conjunction with citrus fruit extract, date palm fruit extract, and gentian root extract, they can achieve better rapid repair and long-term stability. Furthermore, the inventors discovered in actual experiments that the addition of DOTAP cationic lipids can neutralize the negative ions in dipotassium glycyrrhizate, making the liposomes more stable.

[0019] Preferably, the weight ratio of soybean lecithin, cholesterol, phytol, and 1,2-dioleoyl-3-trimethylammonium chloride alanine is soybean lecithin: cholesterol: phytol: 1,2-dioleoyl-3-trimethylammonium chloride alanine = (50-70): (20-40): (5-10): (5-10);

[0020] And / or, the weight ratio of chloroform to ethanol is (2-3):1;

[0021] And / or, the mass-to-volume ratio of the mixture of soybean lecithin, cholesterol, phytol, 1,2-dioleoyl-3-trimethylammonium chloride alanine and solution A is (80-120) mg: 4 mL;

[0022] And / or, the mass concentration of solution B is 1-5 mg / mL; the pH of the phosphate buffer is 5.8-6.2;

[0023] And / or, the weight ratio of phytol to dipotassium glycyrrhizate is 5:(4-25).

[0024] Preferably, the weight ratio of phytol to dipotassium glycyrrhizate is 5:(8-20); more preferably, the weight ratio of phytol to dipotassium glycyrrhizate is 5:(8-12).

[0025] Preferably, the rotary evaporation temperature is 45-60℃, the rotary evaporation speed is 100-200 rpm, and the rotary evaporation time is 30-50 min;

[0026] And / or, the ultrasonic hydration temperature is 50-60℃, the ultrasonic hydration time is 10-30min, and the ultrasonic hydration power is 100-200w;

[0027] And / or, the pressure of the homogenization is 10,000-30,000 psi.

[0028] Furthermore, the present invention provides the use of the described repairing and soothing composition in the preparation of cosmetics. Further, the present invention provides the use of the described repairing and soothing composition in the preparation of cosmetics.

[0029] Furthermore, the present invention provides a cosmetic product comprising the aforementioned repairing and soothing composition.

[0030] Preferably, the cosmetic includes any one of toner, lotion, cream, mask, serum, essential oil, and spray.

[0031] In one specific embodiment, the present invention provides an essence comprising the following components by weight percentage: 1-5% of the repairing and soothing composition, 0.1-1% of a thickener, 0.5-5% of a moisturizer, 0.01-0.3% of a pH adjuster, 0.5-3% of a preservative, and the balance being deionized water.

[0032] Specifically, the thickener includes at least one of the following: polyacrylate crosspolymer-6, carbomer, carrageenan, gellan gum, xanthan gum, microcrystalline cellulose, cellulose gum, ethyl cellulose, argan gum, guar gum, ammonium acryloyldimethyl taurate / VP copolymer, and acrylate copolymers. The humectant includes at least one of the following: glycerin, D-panthenol, vitamin B5, 1,3-butanediol, 1,2-hexanediol, 1,3-propanediol, sodium hyaluronate, tremella polysaccharide, trehalose, betaine, allantoin, and low molecular weight sodium hyaluronate. The pH adjuster includes at least one of the following: arginine, disodium ethylenediaminetetraacetate, tromethamine, and disodium EDTA. The preservative includes at least one of the following: p-hydroxyacetophenone and polyols.

[0033] Compared with the prior art, the beneficial effects of the present invention are as follows: The repair and soothing composition provided by the present invention achieves rapid repair and long-term stabilization through a multi-dimensional synergistic mechanism, specifically manifested as follows: Rapid repair is reflected in the fact that the repair and soothing composition provided by the present invention can relieve postoperative pain, burning sensation, redness and swelling and other discomforts; long-term stabilization is reflected in the fact that the repair and soothing composition provided by the present invention can rebuild the physical barrier and microecological barrier, maintain skin homeostasis in the long term, and cover the entire postoperative repair cycle. Attached Figure Description

[0034] Figure 1 The study investigated the skin improvement of subjects 30 minutes and 28 days after using the serum prepared in Application Example 1 and Control Application Example 4 following phototherapy. Detailed Implementation

[0035] To better illustrate the purpose, technical solution, and advantages of this invention, the invention will be further described below in conjunction with the accompanying drawings and specific embodiments. The purpose is to provide a detailed understanding of the invention, not to limit it. All other embodiments obtained by those skilled in the art without inventive effort are within the scope of protection of this invention. Unless otherwise specified, the experimental reagents and instruments designed in the embodiments and comparative examples of this invention are commonly used reagents and instruments, all of which are commercially available. Unless otherwise specified, the experimental methods used in the embodiments and comparative examples are conventional methods; and unless otherwise specified, the raw materials used in parallel experiments are from the same batch.

[0036] The raw materials of this invention will now be further described, but are not limited to the following raw materials:

[0037] Citrus fruit extract: purchased from Shanghai Jiaye Biotechnology Co., Ltd., under the brand name Yuefuning;

[0038] Date palm fruit extract: purchased from Lucas Meyer, product name [product name missing].

[0039] Gentian root extract: purchased from GANEL Biotech (Shanghai) Co., Ltd.;

[0040] Dipotassium glycyrrhizate: purchased from Gansu Fanzhi Pharmaceutical Co., Ltd.;

[0041] Phytol: Purchased from BASF;

[0042] Kava Pepper Root Extract: Purchased from Ganabio Biotechnology (Shanghai) Co., Ltd., under the trade name Kava Soothing Agent;

[0043] Schisandra chinensis hydrolysate: purchased from Expanscience Laboratoires, France, product name:

[0044] Centella Asiatica extract: Purchased from Shaanxi Xintianyu Biotechnology Co., Ltd.

[0045] Liposome-1: Self-made, prepared as follows:

[0046] (1) Soybean lecithin, cholesterol, phytol, and 1,2-dioleoyl-3-trimethylammonium chloride alanine were mixed and dissolved in solution A. The lipid membrane was obtained by rotary evaporation at 50°C for 30 min. The rotary evaporation speed was 150 rpm.

[0047] The soybean lecithin:cholesterol:phytol:1,2-dioleoyl-3-trimethylammonium chloride alanine ratio is 60:30:5:5; solution A is a mixed solution of chloroform and ethanol, with a weight ratio of chloroform to ethanol of 2:1; the mass-volume ratio of the mixture of soybean lecithin, cholesterol, phytol, 1,2-dioleoyl-3-trimethylammonium chloride alanine and solution A is 100 mg: 4 mL;

[0048] (2) Add solution B to the lipid membrane in step (1), and homogenize it under ultrasonic hydration at 150W and 60℃ for 30 min and pressure of 20000psi to obtain the liposomes; the solution B is a phosphate buffer containing dipotassium glycyrrhizate and the mass concentration of the solution B is 2mg / mL.

[0049] The weight ratio of phytol to dipotassium glycyrrhizate in the raw materials is 5:8.

[0050] Liposome-2: self-made, compared with the preparation method of liposome-1, only the mass concentration and amount of solution B in step (2) are different, the mass concentration of solution B is 2 mg / mL; wherein, the weight ratio of phytol and dipotassium glycyrrhizate in the raw materials is 5:12.

[0051] Liposome-3: self-made, compared with the preparation method of liposome-1, only the mass concentration and amount of solution B in step (2) are different, the mass concentration of solution B is 2 mg / mL; wherein, the weight ratio of phytol and dipotassium glycyrrhizate in the raw materials is 5:20.

[0052] Liposome-4: self-made, compared with the preparation method of liposome-1, only the amount of raw materials in step (1) is different. The soybean lecithin: cholesterol: phytol: 1,2-dioleoyl-3-trimethylammonium chloride alanine = 50:30:10:10; wherein, the weight ratio of phytol and dipotassium glycyrrhizate in the raw materials is 5:4.

[0053] Liposome-5: self-made, compared with the preparation method of liposome-1, only the mass concentration and amount of solution B in step (2) are different, the mass concentration of solution B is 5 mg / mL; wherein, the weight ratio of phytol and dipotassium glycyrrhizate in the raw materials is 5:25.

[0054] Examples and Comparative Examples

[0055] The present invention provides a repairing and soothing composition in the embodiments and comparative examples. The components and weight parts of the repairing and soothing composition are selected as shown in Tables 1-2. The preparation method of the repairing and soothing composition is as follows: the components are mixed evenly to obtain the repairing and soothing composition.

[0056] In Comparative Example 6, phytol and dipotassium glycyrrhizate in the raw materials were not prepared into liposomes. Each liposome component was added separately and directly mixed with the remaining raw materials to obtain the repairing and soothing composition.

[0057] Table 1

[0058]

[0059]

[0060] Table 2

[0061]

[0062]

[0063] Application examples

[0064] The compositions for post-medical aesthetic repair and soothing prepared in the examples and comparative examples were formulated into serums, as shown in Table 3. The compositions used in Application Examples 1-9 were those prepared in Examples 1-9, respectively; the compositions used in Comparative Application Examples 1-9 were those prepared in Comparative Examples 1-9, respectively. For example, the composition used in Application Example 1 was the composition in Example 1, the composition used in Application Example 8 was the composition in Example 8, the composition used in Application Example 10 was the composition in Example 1, and so on. The composition used in Comparative Application Example 1 was the composition prepared in Comparative Example 1.

[0065] Table 3

[0066]

[0067]

[0068] This application provides a method for preparing an essence, the method comprising the following steps:

[0069] (1) Mix the humectant and thickener with water and stir. Heat to 85°C and homogenize at 1300 rpm for 4 min to obtain pre-prepared component A.

[0070] (2) Mix the preservatives and heat them to 60°C to melt them, to obtain pre-prepared component B;

[0071] (3) After cooling the pre-prepared component A to 60°C, add the pre-prepared component B at 300 rpm and stir to mix. Then cool the mixture to below 45°C, add the composition and continue stirring for 8 minutes. Finally, add a pH adjuster to adjust the pH to 5.5-6.0, then stop stirring, discharge the material, and obtain the essence.

[0072] Performance testing - 1. In vitro experiments verified that the composition can improve postoperative burning and itching.

[0073] Cosmetic procedures (such as laser treatments and microneedling) can damage the skin barrier and trigger an acute inflammatory response, leading to redness, swelling, pain, and excessive release of pro-inflammatory factors (such as TNF-α and IL-6). Persistent inflammation exacerbates oxidative stress and cell damage, prolongs the recovery period, and can even cause pigmentation hemorrhage (PIH) or hypertrophic scarring. By inhibiting the synthesis and release of inflammatory mediators, the inflammatory cascade can be blocked, reducing oxidative damage to newly formed tissues from free radicals, while simultaneously alleviating the irritation of nerve endings, thereby rapidly improving postoperative discomfort such as burning and itching. The better the anti-inflammatory effect of the composition, the better the corresponding soothing and repairing effect.

[0074] The specific method is as follows:

[0075] (1) Sample preparation: Weigh 1.00g of the repair and soothing compositions prepared in Examples 1-9 and Comparative Examples 1-9 respectively, dissolve them in PBS buffer (pH 6.8) and bring the volume up to 100mL to obtain a sample working solution with a concentration of 1.0%;

[0076] (2) Cell treatment: Logarithmically growing RAW264.7 cells were seeded in 24-well plates at a cell density of 2 × 10⁻⁶ cells / well. 5 Cells / mL, 0.5mL per well, after plating, mark the top cover of the cell plate and incubate in a CO2 incubator for 24h;

[0077] (3) Sample preparation: After incubation for 24 hours, the supernatant was removed. The model group consisted of 200 μL of LPS (1 μg / mL) + culture medium; the sample group consisted of 200 μL of cell culture medium containing 1.0% of the example or comparative composition + LPS (1 μg / mL).

[0078] (4) Detection method: After the cells were treated for 24 hours, the supernatant was pipetted into a centrifuge tube, centrifuged at 1000 r / min for 5 min, the precipitate was discarded and the supernatant was collected. The changes in the amount of inflammatory factors in the supernatant were detected using a mouse tumor necrosis factor-α ELISA kit (Xinbosheng, EMC102a.96).

[0079] The calculation method is as follows:

[0080] TNF-α inhibition rate % = |(TNF-α amount) 模型组 -TNF-α levels 实验组 )| / TNF-α amount 模型组 ×100%; where, the higher the TNF-α inhibition rate%, the stronger the anti-inflammatory ability;

[0081] The test results are shown in Table 4.

[0082] Table 4

[0083]

[0084]

[0085] As shown in the table above, the repairing and soothing composition prepared in the embodiments of the present invention can effectively inhibit the synthesis and release of inflammatory mediators and effectively improve postoperative burning and itching.

[0086] As can be seen from the comparison of Examples 1-5, the repairing and soothing composition of the present invention, when in a specific weight range such as "0.3-0.8 parts of citrus fruit extract, 0.2-0.3 parts of date fruit extract, 0.1-0.3 parts of gentian root extract, and 0.3-0.5 parts of liposomes", can better inhibit the synthesis and release of inflammatory mediators and effectively improve postoperative burning and itching.

[0087] A comparison of Examples 1 and 3 shows that the specific weight ratios of the citrus fruit extract, date palm fruit extract, and gentian root extract did not significantly differentiate their effects in cell experiments. A comparison of Examples 1-3 shows that when the weight percentage of the liposomes is 30-40%, it can better inhibit the synthesis and release of inflammatory mediators, effectively improving postoperative burning and itching.

[0088] As can be seen from the comparison of Examples 1 and 6-9, the weight ratio of phytol and dipotassium glycyrrhizate in the raw materials for liposome preparation has a certain influence on the synthesis and release of inflammatory mediators. When the weight ratio of phytol to dipotassium glycyrrhizate is 5:(8-12), the inhibitory effect on the synthesis and release of inflammatory mediators is the best.

[0089] As can be seen from the comparison of Examples 1 and Comparative Examples 1-9, the effects of the present invention cannot be achieved when one or two of the components are not added, or when other components are used to supplement the proportions while the components are not added. Similarly, the corresponding effects of the present invention cannot be obtained when other similar components are substituted. Specifically, as can be seen from the comparison of Examples 1 and Comparative Example 6, compared to Examples 1, the comparative examples were not prepared into liposomes. In actual experiments, it was found that when phytol and dipotassium glycyrrhizate were not prepared into liposomes, dipotassium glycyrrhizate and phytol partially degraded and became ineffective, failing to achieve long-term stability. As can be seen from the comparison of Examples 1 and Comparative Example 7, in Comparative Example 7, kava root extract, replacing citrus fruit, could also strongly inhibit TNF-α; however, according to subsequent data on the skin's cooling channels, kava root extract could not activate the TRPM8 skin cooling channel. Comparative Examples 8-9, which replaced some components of the present application with Schisandra chinensis hydrolysate and Centella asiatica extract respectively, failed to achieve synergy with the others, resulting in poor inhibition of the synthesis and release of inflammatory mediators.

[0090] Performance testing - 2 In vitro experiments verified that the composition can activate the TRPM8 skin cooling channel.

[0091] As a temperature-sensitive ion channel, TRPM8, once activated, promotes calcium ion influx and triggers the transmission of cooling signals to the central nervous system, directly inhibiting the thermal pain transmission mediated by TRPV1, thus creating an antagonistic effect between hot and cold signals. This activation not only reduces postoperative inflammatory responses such as erythema and edema, but also maintains a low-temperature epidermal microenvironment due to sustained TRPM8 activation, reducing oxidative stress damage, promoting keratinocyte migration, and accelerating the barrier repair process. Therefore, the better the composition's effect on activating TRPM8 gene expression, the more effectively it soothes and calms the skin after cosmetic procedures, providing a cooling and analgesic effect.

[0092] The specific method is as follows:

[0093] (1) Sample preparation: Weigh 1.00g of the repair and soothing compositions prepared in Examples 1-9 and Comparative Examples 1-9 respectively, dissolve them in PBS buffer (pH 6.8) and bring the volume up to 100mL to obtain a sample working solution with a concentration of 1.0%;

[0094] (2) Cell culture: HaCaT cell suspension (purchased from Guangzhou Customs and preserved by the laboratory) was seeded into 96-well cell culture plates at a density of 2000 cells / well. 100 μL of DMEM high glucose culture medium (Gibco) containing 10% fetal bovine serum was added to each well and cultured for 24 h.

[0095] (3) Sample preparation: After incubating the cells for 6 hours, the supernatant was removed. The blank group was 200 μL of DMEM high glucose culture medium; the sample group was 200 μL of cell culture medium containing 1.0% of the example or comparative composition.

[0096] (4) Collect cells, extract total RNA from cells using TRIzol reagent, and then synthesize cRNA using the PrimeScriptRT reverse transcription kit; then perform relative quantitative analysis of mRNA expression using the CFX96 Touch Real-Time PCR detection system (Bio-Rad, Hercules, CA, USA). The experimental conditions were: initial denaturation at 95℃ for 5 min, followed by 40 cycles of 95℃ / 15s, 60℃ / 30s, and 72℃ / 30s; after normalization to GAPDH transcript levels, the relative cDNA level was calculated using the 2-ΔΔCt method.

[0097] The calculation method is as follows:

[0098] TRPM8 gene change rate % = |(relative cDNA level of each gene in the control group - relative cDNA level of each gene in the sample group)| / relative cDNA level of each gene in the control group × 100%

[0099] The results are shown in Table 5.

[0100] Table 5

[0101]

[0102]

[0103] As can be seen from the table above, the repairing and soothing composition prepared in the embodiments of the present invention can effectively activate the TRPM8 skin cooling channel.

[0104] As can be seen from the comparison of Examples 1-5, the repairing and soothing composition of the present invention can better activate the TRPM8 skin cooling channel when the specific weight ratio of the citrus fruit extract, date palm fruit extract, gentian root extract, and liposomes is within a specific range. Specifically, as can be seen from the comparison of Examples 1 and 3, the specific weight ratio of the citrus fruit extract, date palm fruit extract, and gentian root extract within a specific range can better activate the TRPM8 skin cooling channel. When the weight percentage of the liposomes is 30-40%, the TRPM8 skin cooling channel can be better activated. In cell experiments, as can be seen from the comparison of Examples 1 and Examples 6-9, the weight ratio of phytol and dipotassium glycyrrhizate in the raw materials for liposome preparation has almost no effect on the activation of the TRPM8 skin cooling channel. The reason may be that the activation of the TRPM8 skin cooling channel is a short-term process in this cell experiment. As long as it is prepared into liposomes, it has a good effect on activating the TRPM8 skin cooling channel.

[0105] As can be seen from the comparison of Examples 1 and Comparative Examples 1-9, the effects of the present invention cannot be achieved when one or two of the components are not added, or when other components are used to make up the difference while the components are not added. Similarly, the corresponding effects of the present invention cannot be obtained when other similar components are substituted. Specifically, as can be seen from the comparison of Examples 1 and Comparative Example 6, the comparative examples, compared to Examples 1, are not prepared into liposomes. In actual experiments, it was found that phytol and dipotassium glycyrrhizate, when not prepared into liposomes, cannot synergistically activate the TRPM8 skin cooling channel with other components. Furthermore, as can be seen from the comparison of Examples 1 and Comparative Example 7, the kava root extract, which replaced citrus fruit in Comparative Example 7, could not activate the TRPM8 skin cooling channel. In Comparative Examples 8-9, schisandra hydrolysate and centella asiatica extract, respectively, replaced some components of the present application, but neither achieved synergy with the others, resulting in poor activation of the TRPM8 skin cooling channel.

[0106] Performance testing - 3 Human skin patch test.

[0107] Because skin is extremely sensitive after cosmetic procedures, the safety and gentleness of repair and soothing products are paramount. Therefore, we conducted safety tests on skincare products. We recruited 32 volunteers aged 20-50 and used a closed patch test method. Since arm skin is thicker and tougher than facial skin, to simulate the damaged skin condition of the subjects after cosmetic procedures, we pre-treated the subjects' arms with medical 3M tape, performing five peel-off operations to partially disrupt the skin barrier. Equal volumes (0.020mL-0.025mL) of test samples (serum prepared for application, control, and blank applications) were placed in specific 10-well patch testers, allowing for the use of two 10-well patch testers. Volunteers were divided into groups of 16 and randomly tested on one patch on their left arm. The procedure involved applying the patch to the volunteer's arm with hypoallergenic tape, gently pressing to ensure even application, and leaving it on for 24 hours. The blank control group used distilled water, and the blank application group used a serum without the desired composition. After 24 hours, remove the patch applicator and observe the skin reaction for another 24 hours. Record the results. The severity of adverse skin reactions is shown in Table 6 below, and the test results are shown in Table 7 below.

[0108] Table 6

[0109]

[0110] Table 7

[0111]

[0112]

[0113] As shown in the table above, the serums prepared in the application examples, comparative application examples, and blank application examples of this invention all showed negative reactions.

[0114] Performance Test - 4: The restorative effect of the composition on people after cosmetic surgery.

[0115] Experimental Methods: Following the "Cosmetic Safety Technical Specifications" (2015), subjects aged 20-50 years were selected for a post-photorejuvenation repair and soothing experiment. Twelve groups of three subjects were randomly assigned. All subjects understood the experimental requirements and agreed to undergo photorejuvenation on their faces and complete the test. An M22 photorejuvenation device was selected, and the hospital technician adjusted the intensity according to the subject's specific facial condition and different areas. After the procedure, baseline values ​​were measured, and samples were used for 30 minutes. Volunteers then applied the samples (prepared from application examples 1-5, 7-9, control examples 4, 6, 9, and a blank application example) to their entire face twice daily, morning and evening. Data were collected on days 14 and 28. Upon arrival, volunteers sat quietly for 30 minutes in an air-conditioned room at 21±1℃ and 50±10% humidity. The transepidermal water loss (TEWL) value of the cheekbone was measured using the skin moisture loss test. Photorejuvenation is a non-invasive cosmetic procedure, so post-procedure redness is not visible to the naked eye. Therefore, the VISA-CR instrument was used to photograph the red areas on the subject's face and quantify the degree and area of ​​redness. Specifically, VISA-CR, in cross-polarized light mode, filters out reflected light from the skin surface, capturing the distribution of microvessels and the state of inflammation within 2 millimeters of the subcutaneous layer, making deep redness visible. Image-Pro Plus software was used to analyze the red area image and extract the a* value (red-green axis chromaticity coordinates). The higher the a* value, the more severe the skin redness. The area of ​​redness is obtained by dividing the area of ​​red pixels by the total area analyzed.

[0116] The post-cosmetic procedure barrier repair ability of the composite material is represented by the improvement in TEWL value, and the soothing ability is represented by the degree and area of ​​redness, as shown in the following formula:

[0117] Improvement rate % = |X 使用前均值 -X 使用后均值 | / X 使用前均值 ×100%;

[0118] The results are shown in Table 8.

[0119] Table 8

[0120]

[0121]

[0122] As shown in the table above, the repairing and soothing composition prepared in the embodiments of the present invention, when made into an essence, can effectively improve the barrier repair and soothing effects after phototherapy. Figure 1The study compared the skin improvement of subjects 30 minutes and 28 days after using the serum prepared in Application Example 1 and Comparative Application Example 4 following phototherapy. It can be seen that the repairing and soothing composition prepared in the embodiments of the present invention, when made into a serum, can effectively improve barrier repair and soothe the skin after phototherapy.

[0123] The barrier repair and soothing effects after 30 minutes show that, as demonstrated by comparisons in Application Examples 1-5, the repair and soothing composition of this invention, when within a specific weight range such as "0.3-0.8 parts of citrus fruit extract, 0.2-0.3 parts of date palm fruit extract, 0.1-0.3 parts of gentian root extract, and 0.3-0.5 parts of liposomes," can better improve barrier repair and soothing abilities. Specifically, comparisons between Application Examples 1 and 3 show that when the specific weight ratio of the citrus fruit extract, date palm fruit extract, and gentian root extract is within a specific range, and when the weight percentage of the liposomes is 30-40%, it can better improve barrier repair and soothing abilities. Comparisons between Application Examples 1 and 7-9 show that the weight ratio of phytol and dipotassium glycyrrhizate in the raw materials for liposome preparation has almost no effect on barrier repair and soothing abilities. The reason may be that the activation of the TRPM8 skin cooling channel is a short-term process. As long as it is prepared into liposomes, it can effectively improve barrier repair and soothing. The raw materials of liposomes do not show a significant effect on barrier repair and soothing at 30 minutes.

[0124] As can be seen from the comparison of Application Example 1 and Comparative Application Examples 4, 6, and 9, when one or two components are not added and the proportions of other components are used to make up the difference, or when other similar components are used as substitutes, the corresponding effects of this invention cannot be achieved. Specifically, as can be seen from the comparison of Application Example 1 and Comparative Application Example 6, when phytol and dipotassium glycyrrhizate are not prepared into liposomes, it was found in actual experiments that when phytol and dipotassium glycyrrhizate are not prepared into liposomes, dipotassium glycyrrhizate and phytol degrade and become ineffective, failing to synergize well with other components. Furthermore, in Comparative Application Example 9, replacing the gentian root extract of this application with Centella asiatica extract failed to achieve synergy with components such as citrus fruit extract and date palm fruit extract.

[0125] The barrier repair and soothing effects after 14 and 28 days are evident. Comparing Application Examples 1-5, it is clear that the repair and soothing composition of this invention provides better barrier repair and soothing effects when within a specific weight range, such as "0.3-0.8 parts of citrus fruit extract, 0.2-0.3 parts of date palm fruit extract, 0.1-0.3 parts of gentian root extract, and 0.3-0.5 parts of liposomes". Specifically, comparing Application Examples 1 and 3, it is evident that the specific weight ratios of the citrus fruit extract, date palm fruit extract, and gentian root extract do not significantly differentiate the effects after 14 and 28 days. Comparing Examples 1-3, it is clear that when the weight percentage of liposomes is 30-40%, the barrier repair and soothing effects are better.

[0126] As can be seen from the comparison of Application Examples 1 and 6-9, the weight ratio of phytol and dipotassium glycyrrhizate in the raw materials for liposome preparation has a certain impact on barrier repair and soothing. The barrier repair and soothing effects are best when the weight ratio of phytol to dipotassium glycyrrhizate is 5:(8-12). As can be seen from the comparison of Application Examples 1 and Comparative Application Examples 4, 6, and 9, when one or two of the components are not added and the remaining components are used to make up the difference, or when other similar components are substituted, the corresponding effects of this invention cannot be achieved. Specifically, as can be seen from the comparison of Application Example 1 and Comparative Application Example 6, without preparation into liposomes, dipotassium glycyrrhizate and phytol degrade and become ineffective, failing to achieve long-term stability.

[0127] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A repairing and soothing composition, characterized in that, The composition comprises the following components in parts by weight: 0.3-0.8 parts citrus fruit extract, 0.2-0.3 parts date palm fruit extract, 0.1-0.3 parts gentian root extract, and 0.3-0.5 parts liposomes; the liposomes constitute 30-40% by weight of the total weight of the repairing and soothing composition. The method for preparing the liposomes includes the following steps: (1) Soybean lecithin, cholesterol, phytol, and 1,2-dioleoyl-3-trimethylammonium chloride alanine are mixed and dissolved in solution A, and the lipid membrane is obtained by rotary evaporation; solution A is a mixed solution of chloroform and ethanol; the weight ratio of soybean lecithin, cholesterol, phytol, and 1,2-dioleoyl-3-trimethylammonium chloride alanine is soybean lecithin: cholesterol: phytol: 1,2-dioleoyl-3-trimethylammonium chloride alanine = (50-70): (20-40): (5-10): (5-10); (2) Add solution B to the lipid membrane in step (1), and sonicate and homogenize to obtain the liposomes; the solution B is a phosphate buffer containing dipotassium glycyrrhizate; the mass concentration of the solution B is 1-5 mg / mL; the weight ratio of phytol to dipotassium glycyrrhizate is 5:(4-25).

2. The repairing and soothing composition as described in claim 1, characterized in that, The weight ratio of the citrus fruit extract, date palm fruit extract, and gentian root extract is citrus fruit extract: date palm fruit extract: gentian root extract = (2-3): 1:

1.

3. The repairing and soothing composition as described in claim 1, characterized in that, The weight ratio of chloroform to ethanol is (2-3):1; And / or, the mass-to-volume ratio of the mixture of soybean lecithin, cholesterol, phytol, 1,2-dioleoyl-3-trimethylammonium chloride alanine and solution A is (80-120) mg: 4 mL.

4. The repairing and soothing composition as described in claim 1, characterized in that, The rotary evaporation temperature is 45-60℃, the rotary evaporation speed is 100-200 rpm, and the rotary evaporation time is 30-50 min; And / or, the ultrasonic hydration temperature is 50-60℃, the ultrasonic hydration time is 10-30min, and the ultrasonic hydration power is 100-200w; And / or, the pressure of the homogenization is 10,000-30,000 psi.

5. The use of a repairing and soothing composition as described in any one of claims 1-4 in the preparation of cosmetics.

6. A cosmetic product, characterized in that, The cosmetic product includes the repairing and soothing composition as described in any one of claims 1-4.

7. The cosmetic product as described in claim 6, characterized in that, The cosmetics include any one of the following: toner, lotion, cream, mask, serum, facial oil, and spray.

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