Application of CmPHL1 transcription factor in citrus leaf oil sac formation inhibition and volatile matter regulation

By overexpressing the CmPHL1 transcription factor in citrus, the problem of regulating oil cell development using traditional methods has been solved, enabling precise regulation of oil cell number and volatile matter content. This provides a stable breeding method and gene target, with significant economic and ecological benefits.

CN122146762APending Publication Date: 2026-06-05FUJIAN AGRI & FORESTRY UNIV
0 Cites 0 Cited by

Patent Information

Application Number
CN202610349341.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-03-20
Publication Date
2026-06-05

AI Technical Summary

Technical Problem

Traditional methods are difficult to precisely and specifically regulate the development of citrus oil cells, and may lead to non-target effects or environmental residues. Traditional breeding cycles are long and it is difficult to control the oil cell reduction trait.

Method used

By utilizing the CmPHL1 transcription factor and its encoding gene, a recombinant vector was constructed and introduced into the citrus genome to achieve overexpression of CmPHL1 to negatively regulate oil cell formation and volatile substance content. Genetic transformation and gene editing technologies were then used to stably deliver the reduced oil cell phenotype.

Benefits of technology

It achieves precise intervention in the number of oil cells, reduces the number of oil cells and the content of volatile substances, provides new gene targets and breeding methods, has sustainability and genetic stability, and has significant economic and ecological benefits.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The invention belongs to the technical field of biology, and discloses application of a CmPHL1 transcription factor in inhibiting formation of citrus leaf oil cells and regulating volatile matters. Aiming at the problem of insufficient research on existing citrus oil sac formation and essential oil synthesis, a positive strain is obtained by constructing a pGBI-CmPHL1 overexpression vector and performing agrobacterium tumefaciens-mediated transformation on citrus. Experiments prove that overexpression of CmPHL1 can significantly reduce the number of oil cells and reduce the content of volatile matters such as monoterpenes and sesquiterpenes. The CmPHL1 targeted oil cell development molecular regulation and control network is accurate in regulation and control and inheritable, provides a new gene target for citrus quality improvement and breeding, conforms to the green agriculture direction and has important economic and ecological benefits.
Need to check novelty before this filing date? Find Prior Art