Traditional Chinese medicine for treating pain symptoms and preparation process thereof
Through the preparation processes of boiling, enzymatic decomposition, ethanol extraction and composite extraction, traditional Chinese medicine products with rich active ingredients were prepared, solving the problem of limited efficacy of existing pain treatment drugs and achieving efficient inhibition of inflammation and analgesia.
Patent Information
- Application Number
- CN202510449598.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-10
- Publication Date
- 2025-07-25
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
The current pain-treatment drugs are limited in efficacy, and Western medicine has side effects, while traditional Chinese medicine has slow effect, so it is necessary to provide a Chinese medicine preparation method with good efficacy and small side effects.
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It significantly reduces the content of TNF-α, IL-1β, and IL-6, effectively inhibits inflammation, has good analgesic effects, is simple in preparation technology, and has excellent product effect.
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of traditional Chinese medicine preparation, and particularly to a traditional Chinese medicine for treating pain symptoms and its preparation process. Background Art
[0002] Modern medicine believes that pain is a complex physiological and psychological activity and is one of the most common symptoms in clinical practice. It includes the pain sensation caused by a nociceptive stimulus acting on the body, as well as the pain response of the body to the nociceptive stimulus (somatic motor response and / or visceral vegetative response, often accompanied by strong emotional coloring). Pain sensation can serve as a warning of harm to the body and trigger a series of defensive and protective responses in the body.
[0003] In medicine, the types of pain and their corresponding characteristics are as follows: Distending pain: refers to pain accompanied by a sense of distension or with distension being more severe than pain; it is often characterized by an unfixed location, being aggravated by emotional fluctuations and alleviated by belching or passing flatus, which is the characteristic of pain caused by qi stagnation; for example, distending pain in the chest and hypochondrium or in the epigastrium and abdomen is mostly caused by qi stagnation. However, distending pain in the head is mostly caused by upward inflammation of liver fire or hyperactivity of liver yang. Heavy pain: refers to the symptom of pain accompanied by a feeling of heaviness; it is mostly caused by dampness pathogen obstructing qi movement; due to the heavy and turbid nature of dampness, there is a feeling of heaviness and pain; heavy pain is commonly seen in the head, limbs, waist, and the whole body. Stabbing pain: refers to pain like being pricked by a needle or cut by a knife, often characterized by a relatively fixed location, being more severe at night, which is the characteristic of pain caused by blood stasis; for example, stabbing pain in the chest and hypochondrium, epigastrium and abdomen and other parts is mostly caused by blood stasis blockage and poor blood circulation. Colic pain: refers to the symptom of extremely severe pain like being cut by a knife; it is mostly caused by a tangible pathogenic factor blocking qi movement or cold pathogen congealing qi movement; for example, the "true heart pain" caused by blockage of the heart meridian, the upper abdominal pain caused by stone blockage of the bile duct, and the epigastric pain caused by cold pathogen invading the stomach all have the characteristics of colic pain. Burning pain: refers to the symptom of pain with a burning sensation and a preference for cold; when caused by fire pathogen invading the collaterals, it is a syndrome of excess; when caused by deficiency of yin and excessive fire, it is a syndrome of deficiency. Cold pain: refers to the symptom of pain with a cold sensation and a preference for warmth; it is commonly seen in the lumbar spine, epigastrium and abdomen, and joints of the limbs, etc.; when caused by cold pathogen blocking the meridians, it is a syndrome of excess; when caused by deficiency of yang qi and lack of warming of the zang-fu organs and meridians, it is a syndrome of deficiency. Dull pain: refers to the symptom of pain that is not severe and can be endured, but is continuous; it is mostly caused by deficiency of yang qi, essence and blood, and lack of nourishment of the zang-fu organs and meridians; it is commonly seen in the head, chest, epigastrium, abdomen and other parts. Drawing pain: refers to the symptom of pulling and tugging pain, spreading from one place to another; also known as leading pain or penetrating pain; it is mostly caused by lack of nourishment of the tendons and meridians or blockage of the tendons and meridians.
[0004] Currently, there are a variety of drugs for relieving / treating pain, but their curative effects are limited; although western medicine-related drugs have a quick curative effect, they often have hidden side effects; traditional Chinese medicine is derived from plant components, although it overcomes the defects of western medicine and has fewer side effects, its curative effect is relatively slow, and its medicinal efficacy needs to be improved and adjusted. Therefore, providing a traditional Chinese medicine for treating pain symptoms with good and fast curative effects is a technical problem that needs to be solved currently. Summary of the Invention
[0005] In view of the above technical problems, the present invention provides a technical solution, which includes a traditional Chinese medicine for treating pain symptoms and its preparation process.
[0006] The present invention provides a preparation process for a traditional Chinese medicine for treating pain symptoms, comprising the following preparation steps:
[0007] Step 1: Prepare lycopodium clavatum, aristolochia mollissima, terminalia chebula, strychnos nux-vomica, codonopsis pilosula, eucommia ulmoides, achyranthes bidentata, angelica sinensis, elm bark, white peony root, and moutan bark, mix them, and pulverize to obtain traditional Chinese medicine raw materials;
[0008] Step 2: Mix the traditional Chinese medicine raw materials with water, heat to boiling, cool, and keep stirring for 2 - 6 hours to obtain Product 1;
[0009] Step 3: Cool Product 1, add serrapeptase, stir and enzymatically hydrolyze for 2 - 4 hours, and inactivate the enzyme to obtain Product 2;
[0010] Step 4: Add ethanol to Product 2, adjust the mass concentration of ethanol, and then heat and stir for 3 - 7 hours to obtain Product 3;
[0011] Step 5: Filter Product 3, concentrate under reduced pressure to obtain a concentrate, mix the concentrate with an extractant, stir and extract, let it stand for layering, and take the extracted oil phase to obtain Product 4;
[0012] Step 6: Concentrate Product 4 under reduced pressure until the oil phase solvent cannot be detected to obtain the traditional Chinese medicine for treating pain symptoms.
[0013] Preferably, by weight, 1 - 3 parts of lycopodium clavatum, 1 - 3 parts of aristolochia mollissima, 1 - 3 parts of terminalia chebula, 0.7 - 2.4 parts of strychnos nux-vomica, 0.7 - 2.4 parts of codonopsis pilosula, 0.7 - 2.4 parts of eucommia ulmoides, 0.4 - 1.8 parts of achyranthes bidentata, 0.4 - 1.8 parts of angelica sinensis, 0.4 - 1.8 parts of elm bark, 0.2 - 1.2 parts of white peony root, and 0.2 - 1.2 parts of moutan bark.
[0014] Preferably, in Step 2, the traditional Chinese medicine raw materials and water are mixed at a mass ratio of 1:10 - 50; cool to 60 - 85°C and keep stirring at 60 - 85°C.
[0015] Preferably, in Step 3, the dosage of serrapeptase is 1000 - 1500 u / g of Product 1; the temperature for stirring and enzymatic hydrolysis is 40 - 55°C.
[0016] Preferably, in Step 4, the mass concentration of ethanol is adjusted to 70 - 85%.
[0017] Preferably, in Step 4, the temperature for heating and stirring is 50 - 60°C.
[0018] Preferably, in step 5, it is concentrated under reduced pressure to 0.8 - 1.5 times the mass of the traditional Chinese medicine raw materials.
[0019] Preferably, in step 5, the mass ratio of the concentrate to the extractant is 1:10 - 30.
[0020] Preferably, the extractant is composed of an oil phase and an aqueous phase in a mass ratio of 3 - 5:1.
[0021] Preferably, the oil phase is a solvent composition of ethyl acetate and cyclohexane in a mass ratio of 2 - 4:1.
[0022] Preferably, the aqueous phase is a calcium chloride solution with a mass concentration of 0.2 - 0.8 wt%.
[0023] Preferably, in step 5, the stirring extraction temperature is 30 - 50 °C, the time is 2.5 - 5 hours, and the static separation time is 0.5 - 3 hours.
[0024] Preferably, in step 6, the reduced pressure concentration temperature is 50 - 55 °C, and the pressure is 0.005 - 0.01 MPa.
[0025] The present invention also provides a traditional Chinese medicine for treating pain symptoms prepared according to the said preparation process.
[0026] Preferably, the traditional Chinese medicine for treating pain symptoms is mixed with a solvent and applied to the pain site.
[0027] The beneficial effects of the present invention:
[0028] The present invention uses lycopodium clavatum, aristolochia mollissima, terminalia chebula, strychnos nux - vomica, codonopsis pilosula, eucommia ulmoides, achyranthes bidentata, angelica sinensis, elm bark, white peony root, and moutan bark to prepare a traditional Chinese medicine product through boiling, hot water extraction, serrapeptase enzymolysis, ethanol extraction, concentration, composite extraction with a composite extraction phase, and secondary concentration. The prepared traditional Chinese medicine has a rich variety of active ingredients, high concentration, and few impurities, significantly improving the effect of treating pain symptoms.
[0029] The traditional Chinese medicine prepared by the present invention can effectively reduce the contents of TNF - α, IL - 1β, and IL - 6, can significantly reduce the inflammatory effect caused by pain, and endows the subjects with reduced discomfort caused by inflammation.
[0030] In the preparation system of the present invention, aristolochia mollissima and terminalia chebula are used, and they produce a mutually promoting inhibitory effect on inflammation within the limited dosage range.
[0031] The present invention uses a specific enzyme, serrapeptase, which can improve the anti - inflammatory effect of traditional Chinese medicine to a certain extent and has a better effect compared with other enzymes.
[0032] The present invention uses high - concentration ethanol of 70 - 85% for extraction, which can effectively improve the effect of traditional Chinese medicine in reducing the contents of TNF - α, IL - 1β, and IL - 6.
[0033] The present invention uses ethyl acetate and cyclohexane together as the oil phase of the extractant. Compared with using only one of them, the prepared traditional Chinese medicine has better effects. The two acting together as the extractant can synergistically improve the extraction of active ingredients, making the traditional Chinese medicine more effective.
[0034] When the ratio of ethyl acetate to cyclohexane in the extractant of the present invention is 3 - 5:1, better extraction effects can be obtained, making the efficacy of the traditional Chinese medicine better.
[0035] In the aqueous phase of the extractant of the present invention, a calcium chloride solution with a certain concentration of 0.2 - 0.8 wt% is used, which can better improve the efficacy of the traditional Chinese medicine and make the anti - inflammatory effect better.
[0036] The traditional Chinese medicine prepared by the present invention can effectively inhibit the number of writhing times of mice and has good analgesic effects.
[0037] The preparation process of the present invention is simple, and the comprehensive effects of the product are excellent, having broad application prospects. Specific Embodiments
[0038] The following will elaborate on the present invention in combination with specific embodiments, and various effects of the present invention will be presented more clearly therefrom. Those skilled in the art should understand that these specific embodiments are for explaining the present invention rather than limiting the present invention.
[0039] I. Preparation of Traditional Chinese Medicine
[0040] 1. Traditional Chinese Medicine 1
[0041] The preparation method is as follows: Step 1: Prepare 2.8 parts of Lycopodium clavatum, 2.5 parts of Aristolochia mollissima Hance, 2.5 parts of Terminalia chebula Retz., 2 parts of Strychnos nux - vomica L., 2 parts of Codonopsis pilosula (Franch.) Nannf., 2 parts of Eucommia ulmoides Oliv., 1.5 parts of Achyranthes bidentata Blume, 1.5 parts of Angelica sinensis (Oliv.) Diels, 1.5 parts of Ulmus pumila L. bark, 1 part of Paeonia lactiflora Pall., and 1 part of Paeonia suffruticosa Andr. by mass, mix them, and pulverize to less than 50 meshes to obtain traditional Chinese medicine raw materials;
[0042] Step 2: Mix the traditional Chinese medicine raw materials with water at a mass ratio of 1:15, heat to boiling, and cool to 75 °C for heat preservation stirring at 75 °C and 70 rpm for 4.5 hours to obtain Product 1;
[0043] Step 3: Cool Product 1, add serrapeptase at 1200 u / g of Product 1, stir and enzymatically hydrolyze at 50 °C and 60 rpm for 3.2 hours, and inactivate the enzyme to obtain Product 2;
[0044] Step 4: Add ethanol to Product 2 to adjust the mass concentration of ethanol to 72%, and then heat and stir at 55 °C and 60 rpm for 5.5 hours to obtain Product 3;
[0045] Step 5: Subject the product to triple filtration, and concentrate it under reduced pressure at 60°C and 0.015 Mpa to 1 time the mass of the traditional Chinese medicine raw materials to obtain a concentrate; mix the concentrate with an extractant composed of an oil phase and an aqueous phase in a mass ratio of 4:1 at a mass ratio of 1:24, stir and extract at 45°C and 60 rpm for 4.5 hours, let it stand for liquid separation for 2 hours, and take the extracted oil phase to obtain Product 4;
[0046] The oil phase is a solvent composition of ethyl acetate and cyclohexane in a mass ratio of 3:1; the aqueous phase is a 0.5 wt% calcium chloride solution;
[0047] Step 6: Concentrate Product 4 under reduced pressure at 52°C and 0.008 Mpa until the oil phase solvent cannot be detected to obtain the traditional Chinese medicine for treating pain symptoms.
[0048] 2. Traditional Chinese Medicine 2
[0049] The preparation method is as follows: Step 1: Prepare 2.5 parts of Lycopodium clavatum, 2 parts of Aristolochia mollissima Hance, 2.2 parts of Terminalia chebula Retz., 1.8 parts of Strychnos nux-vomica L., 1.8 parts of Codonopsis pilosula (Franch.) Nannf., 1.8 parts of Eucommia ulmoides Oliv., 1.8 parts of Achyranthes bidentata Blume, 1.7 parts of Angelica sinensis (Oliv.) Diels, 1.5 parts of Ulmus pumila L. bark, 0.8 part of Paeonia lactiflora Pall., and 0.8 part of Paeonia suffruticosa Andr. according to mass parts, mix them, and pulverize them to less than 50 meshes to obtain traditional Chinese medicine raw materials;
[0050] Step 2: Mix the traditional Chinese medicine raw materials with water in a mass ratio of 1:18, heat to boiling, and cool to 75°C for heat preservation stirring at 75°C and 70 rpm for 4.8 hours to obtain Product 1;
[0051] Step 3: Cool Product 1, add serrapeptase at 1300 u / g of Product 1, stir and enzymatically hydrolyze at 52°C and 60 rpm for 3.5 hours, and inactivate the enzyme to obtain Product 2;
[0052] Step 4: Add ethanol to Product 2, adjust the ethanol mass concentration to 85%, and then heat and stir at 52°C and 60 rpm for 5 hours to obtain Product 3;
[0053] Step 5: Subject Product 3 to filtration, concentrate it under reduced pressure at 60°C and 0.015 Mpa to 1 time the mass of the traditional Chinese medicine raw materials to obtain a concentrate; mix the concentrate with an extractant composed of an oil phase and an aqueous phase in a mass ratio of 3:1 at a mass ratio of 1:25, stir and extract at 45°C and 60 rpm for 4.8 hours, let it stand for liquid separation for 2.2 hours, and take the extracted oil phase to obtain Product 4;
[0054] The oil phase is a solvent composition of ethyl acetate and cyclohexane in a mass ratio of 4:1; the aqueous phase is a 0.75 wt% calcium chloride solution;
[0055] Step 6: Concentrate Product 4 under reduced pressure at 54°C and 0.007 Mpa until the oil phase solvent cannot be detected to obtain the traditional Chinese medicine for treating pain symptoms.
[0056] 3. Chinese Medicine 3
[0057] The preparation method is as follows: Step 1: Prepare 2.8 parts of Lycopodium clavatum, 5 parts of Terminalia chebula, 2 parts of Strychnos nux-vomica, 2 parts of Codonopsis pilosula, 2 parts of Eucommia ulmoides, 1.5 parts of Achyranthes bidentata, 1.5 parts of Angelica sinensis, 1.5 parts of Ulmus pumila bark, 1 part of Paeonia lactiflora, and 1 part of Paeonia suffruticosa, mix them, and pulverize to less than 50 mesh to obtain Chinese medicine raw materials;
[0058] Step 2: Mix the Chinese medicine raw materials with water according to a mass ratio of 1:15, heat to boiling, cool to 75°C, and carry out heat preservation stirring at 75°C and 70 rpm for 4.5 hours to obtain Product 1;
[0059] Step 3: Cool Product 1, add Serratia peptidase at 1200 u / g of Product 1, stir and enzymatically hydrolyze at 50°C and 60 rpm for 3.2 hours, and inactivate the enzyme to obtain Product 2;
[0060] Step 4: Add ethanol to Product 2, adjust the ethanol mass concentration to 72%, and then heat and stir at 55°C and 60 rpm for 5.5 hours to obtain Product 3;
[0061] Step 5: Filter Product 3, concentrate it under reduced pressure at 60°C and 0.015 Mpa to 1 time the mass of the Chinese medicine raw materials to obtain a concentrate; mix the concentrate with an extractant composed of an oil phase and a water phase in a mass ratio of 4:1 according to a mass ratio of 1:24, stir and extract at 45°C and 60 rpm for 4.5 hours, let it stand and separate layers for 2 hours, and take the extracted oil phase to obtain Product 4;
[0062] The oil phase is composed of a solvent of ethyl acetate and cyclohexane in a mass ratio of 3:1; the water phase is a 0.5 wt% calcium chloride solution;
[0063] Step 6: Concentrate Product 4 under reduced pressure at 52°C and 0.008 Mpa until the oil phase solvent cannot be detected to obtain the Chinese medicine for treating pain symptoms.
[0064] 4. Chinese Medicine 4
[0065] The preparation method is as follows: Step 1: Prepare 2.8 parts of Lycopodium clavatum, 5 parts of Aristolochia mollissima, 2 parts of Strychnos nux-vomica, 2 parts of Codonopsis pilosula, 2 parts of Eucommia ulmoides, 1.5 parts of Achyranthes bidentata, 1.5 parts of Angelica sinensis, 1.5 parts of Ulmus pumila bark, 1 part of Paeonia lactiflora, and 1 part of Paeonia suffruticosa, mix them, and pulverize to less than 50 mesh to obtain Chinese medicine raw materials;
[0066] Step 2: Mix the Chinese medicine raw materials with water according to a mass ratio of 1:15, heat to boiling, cool to 75°C, and carry out heat preservation stirring at 75°C and 70 rpm for 4.5 hours to obtain Product 1;
[0067] Step 3: After the first product is cooled, add serrapeptase at 1200 u / g of the first product, stir and enzymatically hydrolyze at 50 °C and 60 rpm for 3.2 hours, and inactivate the enzyme to obtain the second product;
[0068] Step 4: Add ethanol to the second product, adjust the mass concentration of ethanol to 72%, and then heat and stir at 55 °C and 60 rpm for 5.5 hours to obtain the third product;
[0069] Step 5: Filter the third product, concentrate it under reduced pressure at 60 °C and 0.015 Mpa to 1 times the mass of the traditional Chinese medicine raw material to obtain a concentrate; mix the concentrate with an extractant composed of an oil phase and a water phase in a mass ratio of 4:1 in a mass ratio of 1:24, stir and extract at 45 °C and 60 rpm for 4.5 hours, let it stand and separate layers for 2 hours, and take the extracted oil phase to obtain the fourth product;
[0070] The oil phase is a solvent composition of ethyl acetate and cyclohexane in a mass ratio of 3:1; the water phase is a 0.5 wt% calcium chloride solution;
[0071] Step 6: Concentrate the fourth product under reduced pressure at 52 °C and 0.008 MPa until the oil phase solvent cannot be detected to obtain the traditional Chinese medicine for treating pain symptoms.
[0072] 5. Traditional Chinese Medicine 5
[0073] The preparation method is as follows: Step 1: According to parts by mass, prepare 2.8 parts of lycopodium clavatum, 2.5 parts of aristolochia mollissima, 2.5 parts of terminalia chebula, 2 parts of strychnos nux-vomica, 2 parts of codonopsis pilosula, 2 parts of eucommia ulmoides, 1.5 parts of achyranthes bidentata, 1.5 parts of angelica sinensis, 1.5 parts of elm bark, 1 part of white peony root, and 1 part of moutan cortex, mix and pulverize to less than 50 mesh to obtain the traditional Chinese medicine raw material;
[0074] Step 2: Mix the traditional Chinese medicine raw material with water in a mass ratio of 1:15, heat to boiling, and cool to 75 °C for heat preservation and stirring at 75 °C and 70 rpm for 4.5 hours to obtain the first product;
[0075] Step 3: After the first product is cooled, add bromelain at 1200 u / g of the first product, stir and enzymatically hydrolyze at 50 °C and 60 rpm for 3.2 hours, and inactivate the enzyme to obtain the second product;
[0076] Step 4: Add ethanol to the second product, adjust the mass concentration of ethanol to 72%, and then heat and stir at 55 °C and 60 rpm for 5.5 hours to obtain the third product;
[0077] Step 5: Filter the third product, concentrate it under reduced pressure at 60 °C and 0.015 Mpa to 1 times the mass of the traditional Chinese medicine raw material to obtain a concentrate; mix the concentrate with an extractant composed of an oil phase and a water phase in a mass ratio of 4:1 in a mass ratio of 1:24, stir and extract at 45 °C and 60 rpm for 4.5 hours, let it stand and separate layers for 2 hours, and take the extracted oil phase to obtain the fourth product;
[0078] The oil phase is a solvent composition of ethyl acetate and cyclohexane in a mass ratio of 3:1; the aqueous phase is a 0.5 wt% calcium chloride solution;
[0079] Step 6: Concentrate the product four under reduced pressure at 52 °C and 0.008 MPa until the oil phase solvent cannot be detected, to obtain the traditional Chinese medicine for treating pain symptoms.
[0080] 6. Traditional Chinese Medicine 6
[0081] The preparation method is as follows: Step 1: Prepare 2.8 parts of Lycopodium clavatum, 2.5 parts of Aristolochia mollissima, 2.5 parts of Terminalia chebula, 2 parts of Strychnos nux-vomica, 2 parts of Codonopsis pilosula, 2 parts of Eucommia ulmoides, 1.5 parts of Achyranthes bidentata, 1.5 parts of Angelica sinensis, 1.5 parts of Ulmus pumila bark, 1 part of Paeonia lactiflora, and 1 part of Paeonia suffruticosa, mix them, and pulverize to less than 50 meshes to obtain traditional Chinese medicine raw materials;
[0082] Step 2: Mix the traditional Chinese medicine raw materials with water in a mass ratio of 1:15, heat to boiling, cool to 75 °C, and carry out heat preservation stirring at 75 °C and 70 rpm for 4.5 hours to obtain product one;
[0083] Step 3: Add ethanol to product one, adjust the ethanol mass concentration to 72%, and then heat and stir at 55 °C and 60 rpm for 5.5 hours to obtain product three;
[0084] Step 5: Filter product three, concentrate it under reduced pressure at 60 °C and 0.015 Mpa to 1 time the mass of the traditional Chinese medicine raw materials to obtain a concentrate; mix the concentrate with an extractant composed of an oil phase and an aqueous phase in a mass ratio of 4:1 in a mass ratio of 1:24, stir and extract at 45 °C and 60 rpm for 4.5 hours, stand for layering for 2 hours, and take the extracted oil phase to obtain product four;
[0085] The oil phase is a solvent composition of ethyl acetate and cyclohexane in a mass ratio of 3:1; the aqueous phase is a 0.5 wt% calcium chloride solution;
[0086] Step 6: Concentrate the product four under reduced pressure at 52 °C and 0.008 MPa until the oil phase solvent cannot be detected, to obtain the traditional Chinese medicine for treating pain symptoms.
[0087] 7. Traditional Chinese Medicine 7
[0088] The preparation method is as follows: Step 1: Prepare 2.8 parts of Lycopodium clavatum, 2.5 parts of Aristolochia mollissima, 2.5 parts of Terminalia chebula, 2 parts of Strychnos nux-vomica, 2 parts of Codonopsis pilosula, 2 parts of Eucommia ulmoides, 1.5 parts of Achyranthes bidentata, 1.5 parts of Angelica sinensis, 1.5 parts of Ulmus pumila bark, 1 part of Paeonia lactiflora, and 1 part of Paeonia suffruticosa, mix them, and pulverize to less than 50 meshes to obtain traditional Chinese medicine raw materials;
[0089] Step 2: Mix the traditional Chinese medicine raw materials and water in a mass ratio of 1:15, heat to boiling, cool to 75°C, and carry out heat preservation stirring at 75°C and 70 rpm for 4.5 hours to obtain Product 1;
[0090] Step 3: Cool Product 1, add serrapeptase at 1200 u / g of Product 1, stir and enzymatically hydrolyze at 50°C and 60 rpm for 3.2 hours, inactivate the enzyme, and obtain Product 2;
[0091] Step 4: Add ethanol to Product 1, adjust the ethanol mass concentration to 40%, and then heat and stir at 55°C and 60 rpm for 5.5 hours to obtain Product 3;
[0092] Step 5: Filter Product 3, concentrate it under reduced pressure at 60°C and 0.015 Mpa to 1 times the mass of the traditional Chinese medicine raw materials to obtain a concentrate; mix the concentrate with an extractant composed of an oil phase and an aqueous phase in a mass ratio of 4:1 in a mass ratio of 1:24, stir and extract at 45°C and 60 rpm for 4.5 hours, let it stand and separate layers for 2 hours, and take the extracted oil phase to obtain Product 4;
[0093] The oil phase is a solvent composed of ethyl acetate and cyclohexane in a mass ratio of 3:1; the aqueous phase is a 0.5 wt% calcium chloride solution;
[0094] Step 6: Concentrate Product 4 under reduced pressure at 52°C and 0.008 Mpa until no oil phase solvent can be detected to obtain the traditional Chinese medicine for treating pain symptoms.
[0095] 8. Traditional Chinese Medicine 8
[0096] The preparation method is as follows: Step 1: According to parts by mass, prepare 2.8 parts of lycopodium clavatum, 2.5 parts of aristolochia mollissima, 2.5 parts of chebula fruit, 2 parts of strychnos nux-vomica, 2 parts of codonopsis pilosula, 2 parts of eucommia ulmoides, 1.5 parts of achyranthes bidentata, 1.5 parts of angelica sinensis, 1.5 parts of elm bark, 1 part of white peony root, and 1 part of moutan bark, mix them, and pulverize them to less than 50 meshes to obtain traditional Chinese medicine raw materials;
[0097] Step 2: Mix the traditional Chinese medicine raw materials and water in a mass ratio of 1:15, heat to boiling, cool to 75°C, and carry out heat preservation stirring at 75°C and 70 rpm for 4.5 hours to obtain Product 1;
[0098] Step 3: Cool Product 1, add serrapeptase at 1200 u / g of Product 1, stir and enzymatically hydrolyze at 50°C and 60 rpm for 3.2 hours, inactivate the enzyme, and obtain Product 2;
[0099] Step 4: Add ethanol to Product 1, adjust the ethanol mass concentration to 72%, and then heat and stir at 55°C and 60 rpm for 5.5 hours to obtain Product 3;
[0100] Step 5: Filter the product three times, concentrate it under reduced pressure at 60°C and 0.015 Mpa to 1 times the mass of the traditional Chinese medicine raw material to obtain a concentrate; mix the concentrate with an extractant composed of an oil phase and an aqueous phase in a mass ratio of 4:1 at a mass ratio of 1:24, stir and extract at 45°C and 60 rpm for 4.5 hours, let it stand for liquid separation for 2 hours, and take the extracted oil phase to obtain product four;
[0101] The oil phase is an ethyl acetate solvent; the aqueous phase is a 0.5 wt% calcium chloride solution;
[0102] Step 6: Concentrate product four under reduced pressure at 52°C and 0.008 Mpa until the oil phase solvent cannot be detected to obtain the traditional Chinese medicine for treating pain symptoms.
[0103] 9. Traditional Chinese Medicine 9
[0104] The preparation method is as follows: Step 1: Prepare 2.8 parts of Lycopodium clavatum, 2.5 parts of Aristolochia mollissima Hance, 2.5 parts of Terminalia chebula Retz., 2 parts of Strychnos nux-vomica L., 2 parts of Codonopsis pilosula (Franch.) Nannf., 2 parts of Eucommia ulmoides Oliv., 1.5 parts of Achyranthes bidentata Blume, 1.5 parts of Angelica sinensis (Oliv.) Diels, 1.5 parts of Ulmus pumila L. bark, 1 part of Paeonia lactiflora Pall., and 1 part of Paeonia suffruticosa Andr. by mass, mix them, and pulverize them to less than 50 meshes to obtain a traditional Chinese medicine raw material;
[0105] Step 2: Mix the traditional Chinese medicine raw material with water at a mass ratio of 1:15, heat to boiling, cool to 75°C, and carry out heat preservation stirring at 75°C and 70 rpm for 4.5 hours to obtain product one;
[0106] Step 3: Cool product one, add serrapeptase at 1200 u / g of product one, stir and enzymolyze at 50°C and 60 rpm for 3.2 hours, and inactivate the enzyme to obtain product two;
[0107] Step 4: Add ethanol to product one, adjust the ethanol mass concentration to 72%, and then heat and stir at 55°C and 60 rpm for 5.5 hours to obtain product three;
[0108] Step 5: Filter the product three times, concentrate it under reduced pressure at 60°C and 0.015 Mpa to 1 times the mass of the traditional Chinese medicine raw material to obtain a concentrate; mix the concentrate with an extractant composed of an oil phase and an aqueous phase in a mass ratio of 4:1 at a mass ratio of 1:24, stir and extract at 45°C and 60 rpm for 4.5 hours, let it stand for liquid separation for 2 hours, and take the extracted oil phase to obtain product four;
[0109] The oil phase is a cyclohexane solvent; the aqueous phase is a 0.5 wt% calcium chloride solution;
[0110] Step 6: Concentrate product four under reduced pressure at 52°C and 0.008 Mpa until the oil phase solvent cannot be detected to obtain the traditional Chinese medicine for treating pain symptoms.
[0111] 10. Traditional Chinese Medicine 10
[0112] The preparation method is as follows: Step 1: Prepare 2.8 parts of Lycopodiella cernua, 2.5 parts of Aristolochia mollissima Hance, 2.5 parts of Terminalia chebula Retz., 2 parts of Strychnos nux-vomica L., 2 parts of Codonopsis pilosula (Franch.) Nannf., 2 parts of Eucommia ulmoides Oliv., 1.5 parts of Achyranthes bidentata Blume, 1.5 parts of Angelica sinensis (Oliv.) Diels, 1.5 parts of Ulmus pumila L. bark, 1 part of Paeonia lactiflora Pall., and 1 part of Paeonia suffruticosa Andr. by mass, mix them, and pulverize to less than 50 mesh to obtain traditional Chinese medicine raw materials;
[0113] Step 2: Mix the traditional Chinese medicine raw materials with water according to a mass ratio of 1:15, heat to boiling, cool to 75°C, and perform heat preservation stirring at 75°C and 70 rpm for 4.5 hours to obtain Product 1;
[0114] Step 3: Cool Product 1, add Serratia peptidase at 1200 u / g of Product 1, stir and enzymatically hydrolyze at 50°C and 60 rpm for 3.2 hours, and inactivate the enzyme to obtain Product 2;
[0115] Step 4: Add ethanol to Product 1, adjust the mass concentration of ethanol to 72%, and then heat and stir at 55°C and 60 rpm for 5.5 hours to obtain Product 3;
[0116] Step 5: Filter Product 3, concentrate it under reduced pressure at 60°C and 0.015 Mpa to 1 times the mass of the traditional Chinese medicine raw materials to obtain a concentrate; mix the concentrate with an extractant composed of an oil phase and an aqueous phase in a mass ratio of 4:1 according to a mass ratio of 1:24, stir and extract at 45°C and 60 rpm for 4.5 hours, let it stand and separate layers for 2 hours, and take the oil phase after extraction to obtain Product 4;
[0117] The oil phase is a chloroform solvent; the aqueous phase is a 0.5 wt% calcium chloride solution;
[0118] Step 6: Concentrate Product 4 under reduced pressure at 52°C and 0.008 Mpa until the oil phase solvent cannot be detected to obtain the traditional Chinese medicine for treating pain symptoms.
[0119] 11. Traditional Chinese Medicine 11
[0120] The preparation method is as follows: Step 1: Prepare 2.8 parts of Lycopodiella cernua, 2.5 parts of Aristolochia mollissima Hance, 2.5 parts of Terminalia chebula Retz., 2 parts of Strychnos nux-vomica L., 2 parts of Codonopsis pilosula (Franch.) Nannf., 2 parts of Eucommia ulmoides Oliv., 1.5 parts of Achyranthes bidentata Blume, 1.5 parts of Angelica sinensis (Oliv.) Diels, 1.5 parts of Ulmus pumila L. bark, 1 part of Paeonia lactiflora Pall., and 1 part of Paeonia suffruticosa Andr. by mass, mix them, and pulverize to less than 50 mesh to obtain traditional Chinese medicine raw materials;
[0121] Step 2: Mix the traditional Chinese medicine raw materials with water according to a mass ratio of 1:15, heat to boiling, cool to 75°C, and perform heat preservation stirring at 75°C and 70 rpm for 4.5 hours to obtain Product 1;
[0122] Step 3: Cool Product 1, add Serratia peptidase at 1200 u / g of Product 1, stir and enzymatically hydrolyze at 50°C and 60 rpm for 3.2 hours, and inactivate the enzyme to obtain Product 2;
[0123] Step 4: Add ethanol to Product 1, adjust the mass concentration of ethanol to 72%, then heat and stir at 55 °C and 60 rpm for 5.5 hours to obtain Product 3;
[0124] Step 5: Filter Product 3, concentrate it under reduced pressure at 60 °C and 0.015 Mpa to 1 times the mass of the traditional Chinese medicine raw material to obtain a concentrate; mix the concentrate with an extractant composed of an oil phase and a water phase in a mass ratio of 4:1 at a mass ratio of 1:24, stir and extract at 45 °C and 60 rpm for 4.5 hours, let it stand for liquid separation for 2 hours, and take the extracted oil phase to obtain Product 4;
[0125] The oil phase is a solvent composition of ethyl acetate and cyclohexane in a mass ratio of 0.5:1; the water phase is a 0.5 wt% calcium chloride solution;
[0126] Step 6: Concentrate Product 4 under reduced pressure at 52 °C and 0.008 MPa until the oil phase solvent cannot be detected to obtain the traditional Chinese medicine for treating pain symptoms.
[0127] 12. Traditional Chinese Medicine 12
[0128] The preparation method is as follows: Step 1: Prepare 2.8 parts of Lycopodium clavatum, 2.5 parts of Aristolochia mollissima Hance, 2.5 parts of Terminalia chebula Retz., 2 parts of Strychnos nux-vomica L., 2 parts of Codonopsis pilosula (Franch.) Nannf., 2 parts of Eucommia ulmoides Oliv., 1.5 parts of Achyranthes bidentata Blume, 1.5 parts of Angelica sinensis (Oliv.) Diels, 1.5 parts of Ulmus pumila L. bark, 1 part of Paeonia lactiflora Pall., and 1 part of Paeonia suffruticosa Andr. by mass, mix them, and pulverize them to less than 50 mesh to obtain the traditional Chinese medicine raw material;
[0129] Step 2: Mix the traditional Chinese medicine raw material with water in a mass ratio of 1:15, heat to boiling, cool to 75 °C, and carry out heat preservation stirring at 75 °C and 70 rpm for 4.5 hours to obtain Product 1;
[0130] Step 3: Cool Product 1, add serrapeptase at 1200 u / g of Product 1, stir and enzymatically hydrolyze at 50 °C and 60 rpm for 3.2 hours, and inactivate the enzyme to obtain Product 2;
[0131] Step 4: Add ethanol to Product 1, adjust the mass concentration of ethanol to 72%, then heat and stir at 55 °C and 60 rpm for 5.5 hours to obtain Product 3;
[0132] Step 5: Filter Product 3, concentrate it under reduced pressure at 60 °C and 0.015 Mpa to 1 times the mass of the traditional Chinese medicine raw material to obtain a concentrate; mix the concentrate with an extractant composed of an oil phase and a water phase in a mass ratio of 4:1 at a mass ratio of 1:24, stir and extract at 45 °C and 60 rpm for 4.5 hours, let it stand for liquid separation for 2 hours, and take the extracted oil phase to obtain Product 4;
[0133] The oil phase is a solvent composition of ethyl acetate and cyclohexane in a mass ratio of 8:1; the water phase is a 0.5 wt% calcium chloride solution;
[0134] Step 6: Concentrate the product under reduced pressure at 52°C and 0.008 MPa until no oil-phase solvent can be detected, obtaining the traditional Chinese medicine for treating pain symptoms.
[0135] 13. Traditional Chinese Medicine 13
[0136] The preparation method is as follows: Step 1: Prepare 2.8 parts of Lycopodium clavatum, 2.5 parts of Aristolochia mollissima Hance, 2.5 parts of Terminalia chebula Retz., 2 parts of Strychnos nux-vomica L., 2 parts of Codonopsis pilosula (Franch.) Nannf., 2 parts of Eucommia ulmoides Oliv., 1.5 parts of Achyranthes bidentata Blume, 1.5 parts of Angelica sinensis (Oliv.) Diels, 1.5 parts of Ulmus pumila L. bark, 1 part of Paeonia lactiflora Pall., and 1 part of Paeonia suffruticosa Andr. by mass, mix them, and pulverize to less than 50 mesh to obtain traditional Chinese medicine raw materials.
[0137] Step 2: Mix the traditional Chinese medicine raw materials with water at a mass ratio of 1:15, heat to boiling, cool to 75°C, and perform heat preservation stirring at 75°C and 70 rpm for 4.5 hours to obtain Product 1.
[0138] Step 3: Cool Product 1, add serrapeptase at 1200 u / g of Product 1, stir and enzymatically hydrolyze at 50°C and 60 rpm for 3.2 hours, and inactivate the enzyme to obtain Product 2.
[0139] Step 4: Add ethanol to Product 1, adjust the ethanol mass concentration to 72%, and then heat and stir at 55°C and 60 rpm for 5.5 hours to obtain Product 3.
[0140] Step 5: Filter Product 3, concentrate it under reduced pressure at 60°C and 0.015 Mpa to 1 times the mass of the traditional Chinese medicine raw materials to obtain a concentrate; mix the concentrate with an extractant composed of an oil phase and an aqueous phase at a mass ratio of 4:1 at a mass ratio of 1:24, stir and extract at 45°C and 60 rpm for 4.5 hours, let it stand and separate layers for 2 hours, and take the extracted oil phase to obtain Product 4.
[0141] The oil phase is a solvent composed of ethyl acetate and cyclohexane at a mass ratio of 3:1; the aqueous phase is a 0.05 wt% calcium chloride solution.
[0142] Step 6: Concentrate the product under reduced pressure at 52°C and 0.008 MPa until no oil-phase solvent can be detected, obtaining the traditional Chinese medicine for treating pain symptoms.
[0143] II. Test on the effect of the traditional Chinese medicine
[0144] 1. Anti-inflammatory test - Inhibiting inflammatory factor experimental test
[0145] Before the test, dilute the above traditional Chinese medicine to a mass concentration of 1% with 2 wt% Tween-60, and then use it as a traditional Chinese medicine sample for testing. The specific experimental test steps are as follows: Inoculate RAW264.7 cells in the logarithmic growth phase on a 96-well plate, and the inoculation concentration and inoculation amount are 5.5×104 At a density of 1 / mL and 200 μL / well, culture in the dark at 37 °C for 24 h; for the blank group and the model group, add the culture medium, and for the Chinese medicine groups 1 - 13, in addition to adding the culture medium, sequentially add the diluted solutions (Chinese medicine samples) of the above Chinese medicines 1 - 13 until the concentration of the Chinese medicine reaches 8 μg / mL; incubate each group for 2 hours, then except for the blank group, add lipopolysaccharide LPS to each group to a medium concentration of 1.2 μg / mL, and act for 24 hours. Use a kit to measure the concentrations of inflammatory mediators tumor necrosis factor (TNF-α), interleukin (IL-1β, IL-6) in each group. The results are shown in Table 1.
[0146] Table 1: Contents of TNF-α, IL-1β, IL-6
[0147] Test / pg / mL TNF-α / pg / mL IL-1β / pg / mL IL-6 / pg / mL Blank group 30.25±1.97 29.76±2.25 6.12±0.29 Model group 78.46±4.83 91.52±5.91 27.58±1.42 Traditional Chinese Medicine 1 38.03±2.61 40.37±2.36 12.69±0.86 Traditional Chinese Medicine 2 39.62±3.02 42.49±3.05 13.38±0.75 Traditional Chinese Medicine 3 40.85±2.51 44.14±2.81 13.84±1.03 Traditional Chinese Medicine 4 42.49±2.98 46.33±3.19 14.45±0.91 Traditional Chinese Medicine 5 49.24±3.74 55.40±3.62 16.92±1.14 Traditional Chinese Medicine 6 52.30±2.57 59.41±3.93 18.07±0.81 Traditional Chinese Medicine 7 57.17±2.34 65.93±4.07 19.86±1.27 Traditional Chinese Medicine 8 45.61±3.01 50.49±3.84 15.59±0.62 Traditional Chinese Medicine 9 48.25±2.73 54.01±2.75 16.56±0.77 Traditional Chinese Medicine 10 52.17±2.65 59.26±4.33 18.01±0.94 Traditional Chinese Medicine 11 41.39±2.15 44.85±3.51 14.03±1.19 Traditional Chinese Medicine 12 43.06±2.27 47.10±3.90 14.65±0.62 Traditional Chinese Medicine 13 44.98±3.18 49.65±3.28 15.37±1.08
[0148] After converting the calculated values in Table 1, calculate the reduction amount of TNF-α, IL-1β, IL-6 contents in each group compared with the model group in pg / mL. The results are shown in Table 2.
[0149] Table 2: Reduction amounts of TNF-α, IL-1β, IL-6 contents in pg / mL
[0150] Test / pg / mL TNF-α Reduction IL-1β Reduction IL-6 Reduction Model group / / / Traditional Chinese Medicine 1 40.43 51.15 14.79 Traditional Chinese Medicine 2 38.84 49.03 14.20 Traditional Chinese Medicine 3 37.61 47.38 13.74 Traditional Chinese Medicine 4 35.97 45.19 13.13 Traditional Chinese Medicine 5 29.22 36.12 10.66 Traditional Chinese Medicine 6 26.16 32.11 9.51 Traditional Chinese Medicine 7 21.29 25.59 7.72 Traditional Chinese Medicine 8 32.85 41.03 11.99 Traditional Chinese Medicine 9 30.21 37.51 11.02 Traditional Chinese Medicine 10 26.29 32.26 9.57 Traditional Chinese Medicine 11 37.07 46.67 13.55 Traditional Chinese Medicine 12 35.40 44.42 12.93 Traditional Chinese Medicine 13 33.48 41.87 12.21
[0151] According to the test results in Tables 1 - 2, it can be seen that the Chinese medicine prepared by the present invention can effectively reduce the contents of TNF-α, IL-1β, IL-6, can significantly reduce the inflammatory effect caused by pain, and endow the subjects with reduced discomfort caused by inflammation.
[0152] According to the results of Chinese medicines 1, 3 - 4, it can be seen that Sarcandra glabra and Terminalia chebula are used in the preparation system of the present invention, and they produce a mutually promoting inhibitory effect on inflammation within the limited dosage range.
[0153] According to the tests of Chinese medicines 1, 5 - 6, it can be known that using the specific enzyme serrapeptase can improve the anti-inflammatory effect of Chinese medicine to a certain extent, and has a better effect compared with other enzymes.
[0154] According to the tests of Chinese medicines 1, 7, it can be known that extraction with 70 - 85% high-concentration ethanol can effectively improve the effect of Chinese medicine in reducing the contents of TNF-α, IL-1β, IL-6.
[0155] According to the tests of Chinese medicines 1, 8 - 10, it can be known that using ethyl acetate and cyclohexane together as the oil phase of the extractant, compared with using only one of them, the prepared Chinese medicine has a better effect. The two together as the extractant can synergistically improve the extraction of active ingredients, making the effect of Chinese medicine better.
[0156] According to the tests of Traditional Chinese Medicine 1, 11 - 12, it is known that when the ratio of ethyl acetate to cyclohexane in the extractant is 3 - 5:1, a better extraction effect can be achieved, making the efficacy of the Traditional Chinese Medicine better.
[0157] According to the test results of Traditional Chinese Medicine 1, 13, it is known that using a calcium chloride solution with a certain concentration of 0.2 - 0.8 wt% in the aqueous phase of the extractant can better enhance the efficacy of the Traditional Chinese Medicine, making the anti - inflammatory effect better.
[0158] 2. Analgesic test - Mouse writhing test
[0159] Before the test, the above - mentioned Traditional Chinese Medicine was diluted to a mass concentration of 20% with a diluent composed of 3 wt% Tween - 60, 10 wt% glycerol and the balance deionized water as the administration sample for the Traditional Chinese Medicine group. The specific experimental test steps are as follows: Select 20 ± 2 g SPF - grade male Kunming mice, raise them for 1 week, and randomly divide them into 5 groups, with 10 mice in each group; The grouping situation is: a positive control group administered with diclofenac diethylamine emulsion (Novartis Pharma AG), Traditional Chinese Medicine groups 1 - 2 administered with the above - mentioned Traditional Chinese Medicine 1 - 2 administration samples, a blank group and a model group. The Traditional Chinese Medicine 1 - 2 groups respectively applied 1.3 g of the administration sample on the abdomen of the mice; The positive control group applied 0.55 g of diclofenac diethylamine emulsion on the abdomen; The blank group and the model group applied 1.3 g of normal saline. 60 minutes after administration, except for the blank group, each group of mice was intraperitoneally injected with 0.65 wt% glacial acetic acid at 10 mL / kg, and the blank group was injected with an equal amount of normal saline. Observe the total number of writhing reactions of the mice within half an hour after the appearance of writhing reactions; Each group was tested 5 times repeatedly. The test results are shown in Table 3.
[0160] Table 3: Analgesic test
[0161] Group Writhing times Blank group 2.21±0.34 Model group 44.36±5.28 Positive control group 12.78±1.05 Traditional Chinese Medicine 1 14.13±1.67 Traditional Chinese Medicine 2 15.94±1.43
[0162] According to the tests in Table 3, it is known that the Traditional Chinese Medicine prepared by the present invention can effectively inhibit the number of writhing reactions of mice and has a good analgesic effect.
[0163] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and its equivalent technologies, the present invention is also intended to include these changes and modifications.
Claims
1. A preparation process of a traditional Chinese medicine for treating pain symptoms, characterized in that, It includes the following preparation steps: Step 1: Prepare lycopodium clavatum, aristolochia mollissima, terminalia chebula, strychnos nux-vomica, codonopsis pilosula, eucommia ulmoides, achyranthes bidentata, angelica sinensis, elm bark, white peony root, and moutan cortex, mix them, and pulverize to obtain traditional Chinese medicine raw materials; Step 2: Mix the traditional Chinese medicine raw materials with water, heat to boiling, cool and keep stirring for 2 - 6 hours to obtain Product 1; Step 3: Cool Product 1, add serrapeptase, stir and enzymatically hydrolyze for 2 - 4 hours, and inactivate the enzyme to obtain Product 2; Step 4: Add ethanol to Product 2, adjust the mass concentration of ethanol, and then heat and stir for 3 - 7 hours to obtain Product 3; Step 5: Filter Product 3, concentrate under reduced pressure to obtain a concentrate, mix the concentrate with an extractant, stir and extract, let it stand for layering, and take the extracted oil phase to obtain Product 4; Step 6: Concentrate Product 4 under reduced pressure until the oil phase solvent cannot be detected to obtain the traditional Chinese medicine for treating pain symptoms.
2. The preparation process of the traditional Chinese medicine for treating pain symptoms according to claim 1, characterized in that, By weight, 1 - 3 parts of lycopodium clavatum, 1 - 3 parts of aristolochia mollissima, 1 - 3 parts of terminalia chebula, 0.7 - 2.4 parts of strychnos nux-vomica, 0.7 - 2.4 parts of codonopsis pilosula, 0.7 - 2.4 parts of eucommia ulmoides, 0.4 - 1.8 parts of achyranthes bidentata, 0.4 - 1.8 parts of angelica sinensis, 0.4 - 1.8 parts of elm bark, 0.2 - 1.2 parts of white peony root, and 0.2 - 1.2 parts of moutan cortex.
3. The preparation process of the traditional Chinese medicine for treating pain symptoms according to claim 1, wherein, In Step 2, the traditional Chinese medicine raw materials and water are mixed at a mass ratio of 1:10 - 50; cool to 60 - 85 °C and keep stirring at 60 - 85 °C.
4. The preparation process of the traditional Chinese medicine for treating pain symptoms according to claim 1, characterized in that, In Step 3, the dosage of serrapeptase is 1000 - 1500 u / g of Product 1; And / or, the temperature of stirring and enzymatic hydrolysis is 40 - 55 °C.
5. The preparation process of the traditional Chinese medicine for treating pain symptoms according to claim 1, characterized in that, In Step 4, adjust the mass concentration of ethanol to 70 - 85%; And / or, in Step 4, the temperature of heating and stirring is 50 - 60 °C.
6. The preparation process of the traditional Chinese medicine for treating pain symptoms according to claim 1, characterized in that, In Step 5, the mass ratio of the concentrate to the extractant is 1:10 - 30; And / or, the extractant is composed of an oil phase and an aqueous phase at a mass ratio of 3 - 5:
1. The oil phase is a solvent composed of ethyl acetate and cyclohexane at a mass ratio of 2 - 4:1, and the aqueous phase is a calcium chloride solution with a mass concentration of 0.2 - 0.8 wt%.
7. The preparation process of the traditional Chinese medicine for treating pain symptoms according to claim 1, characterized in that, In Step 5, the stirring and extraction time is 2.5 - 5 hours; the standing and layering time is 0.5 - 3 hours.
8. The preparation process of the traditional Chinese medicine for treating pain symptoms according to claim 1, characterized in that, In Step 6, the temperature of concentration under reduced pressure is 50 - 55 °C, and the pressure is 0.005 - 0.01 MPa.
9. A traditional Chinese medicine for treating pain symptoms prepared by the preparation process according to any one of claims 1 - 8.
10. The traditional Chinese medicine for treating pain symptoms according to claim 9, wherein, Mix the traditional Chinese medicine for treating pain symptoms with a solvent and apply it to the painful area.