Method for constructing animal insufficiency model based on'long mulberry monarch vein method ', application and animal insufficiency model

Abstract

The invention belongs to the technical field of traditional Chinese medicine pulse diagnosis, and particularly relates to a method for constructing an animal insufficiency qi model based on a long mulberry monarch vein method, application and the animal insufficiency qi model.The method comprises the steps that experimental animals with the pulse-to-rest ratio basically consistent with that of normal people are selected, and conventional feeding is conducted on the experimental animals; during the conventional feeding period, the pulse frequency and the breathing frequency are detected every day, and the pulse-to-rest ratio every day is calculated and ranges from 4 to 5; after conventional feeding is finished, chronic hypoxia is conducted on the experimental animals for 12-16 d, continuous hypoxia is conducted every day for 3-4 h, the concentration of oxygen inhaled by the experimental animals is controlled to be 15 + / -0.5% during continuous hypoxia every day, the pathological process of chronic hypoxia is achieved, the pulse frequency and the breathing frequency of the experimental animals are detected every day in the chronic hypoxia period, the pulse-to-rest ratio of the experimental animals is calculated every day, and if the pulse-to-rest ratio of the experimental animals is smaller than 4, the experimental animals are fed to the experimental animals. According to the method, a stable experimental platform is provided for the subsequent research on the application of the long mulberry monarch vein method in the state.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicine pulse diagnosis, and particularly relates to a method for constructing an animal model of qi deficiency based on the "Pulse Method of Chang Sang Jun", its application, and the animal model of qi deficiency. Background Technique

[0002] The pulse rate technique of the Pulse Method of Chang Sang Jun is a pulse method theory summarized under the guidance of the thought of "Huangdi Neijing". By measuring the ratio of the number of a person's pulse beats to the number of breaths in one minute (referred to as the "pulse-breath ratio"), three types, namely "qi deficiency", "normal person", and "pulse restlessness", can be distinguished. Traditional Chinese medicine believes that "breath" refers to breathing and is related to a person's "qi"; "pulse" is the residence of blood and is related to blood. The pulse-breath ratio is the external manifestation of the qi and blood in the human body and is dynamically changing, while qi and blood are the material basis for human life activities. Therefore, the pulse-breath ratio can objectively and accurately reflect the state of qi and blood deficiency or excess in the human body. According to the ratio of the pulse-breath ratio (qi deficiency < 4, normal person 4 - 5, pulse restlessness > 5), corresponding prescriptions and acupuncture treatments are given to transform the patient's pulse-breath ratio from the state of qi deficiency or pulse restlessness to the state of a normal person. Clinically, there have been documented and proven the effectiveness of the pulse rate technique of the Pulse Method of Chang Sang Jun. For example, good results have been achieved in the treatment of diseases such as diabetic constipation and acute pancreatitis.

[0003] Animal models are important tools in medical research. They can help researchers deeply understand the pathogenesis of diseases, verify the effectiveness of treatment methods, and provide experimental basis for new drug development. In the field of traditional Chinese medicine, the establishment of animal models is of great significance for revealing the scientific connotations of traditional Chinese medicine theories and promoting the modernization process of traditional Chinese medicine.

[0004] Although the effectiveness of the pulse rate technique of the Pulse Method of Chang Sang Jun has been proven, the current mechanism research still lacks a reliable animal model based on traditional Chinese medicine pulse diagnosis theory. Especially for the animal model related to this unique pulse diagnosis method of the Pulse Method of Chang Sang Jun, the lack of the animal model of the Pulse Method of Chang Sang Jun pulse rate technique has restricted the in-depth research on the Pulse Method of Chang Sang Jun pulse rate technique, especially the exploration of its action mechanism, efficacy evaluation, etc. Summary of the Invention

[0005] In view of this, the present invention provides a method for constructing an animal model of qi deficiency based on the "Pulse Method of Chang Sang Jun", its application, and the animal model of qi deficiency, so as to solve the technical problem in the prior art that the lack of the animal model of the Pulse Method of Chang Sang Jun pulse rate technique has restricted the in-depth research on the Pulse Method of Chang Sang Jun pulse rate technique.

[0006] To achieve the above object, the present application adopts the following scheme:

[0007] A method for constructing an animal model of qi deficiency based on the "Pulse Method of Chang Sang Jun" includes the following steps:

[0008] S10. Select experimental animals, and the normal pulse-breath ratio of the experimental animals is basically the same as that of normal people;

[0009] S20. The experimental animals are conventionally raised, and during the conventional raising period, the number of pulse beats and the number of breaths of the experimental animals are detected daily, and the pulse-breath ratio of each day is calculated, and the pulse-breath ratio of each day is between 4 and 5.

[0010] S30. After the conventional raising is completed, the experimental animals are subjected to chronic hypoxia for 12 d to 16 d, with continuous hypoxia for 3 h to 4 h every day. During the continuous hypoxia every day, the oxygen concentration inhaled by the experimental animals is controlled to be 15 ± 0.5%, so as to realize the pathological process of chronic hypoxia. During the chronic hypoxia period, the number of pulse beats and the number of breaths of the experimental animals are detected daily, and the pulse-breath ratio of each day is calculated. If the pulse-breath ratio of each day is less than 4, it indicates that the modeling is successful.

[0011] Preferably, the experimental animals are of the rabbit breed.

[0012] Preferably, the experimental animals are New Zealand rabbits.

[0013] Preferably, the experimental animals are conventionally raised for 5 to 10 d.

[0014] Preferably, the experimental animals are subjected to chronic hypoxia for 14 d, with continuous hypoxia for 4 h every day.

[0015] Preferably, a chronic hypoxia experimental device with adjustable oxygen concentration can be used to subject the experimental animals to chronic hypoxia. The chronic hypoxia experimental device with adjustable oxygen concentration includes: a water tank box with an opening at the top, a hypoxia box with an opening at the bottom, an oxygen concentration detector, and an oxygen concentration adjustment component. The length and width of the water tank box are respectively greater than the length and width of the hypoxia box. An animal platform is arranged in the water tank box. The hypoxia box is covered in the water tank box and covers the animal platform inside. The oxygen concentration detector is arranged in the hypoxia box and is used to detect the oxygen concentration in the hypoxia box. The oxygen adjustment component includes an oxygen delivery piece and a vacuum pump. One end of the oxygen delivery piece passes through the box body of the hypoxia box and is located inside the hypoxia box. The other end of the oxygen delivery piece is connected to the vacuum pump. At least one air exchange hole is opened on the box body of the hypoxia box, and each air exchange hole is equipped with a rubber soft plug, and the rubber soft plug can be tightly inserted into the air exchange hole.

[0016] Preferably, the S30 specifically includes the following steps:

[0017] S31. Put the experimental animals on the animal platform.

[0018] S32. Place the hypoxia box in the water tank box, add water to a certain height in the water tank box, and seal the hypoxia box by the water seal method.

[0019] S33. To rapidly reduce the oxygen concentration in the hypoxic chamber, a part of the air in the hypoxic chamber is extracted using a vacuum pump and a conveying member. When the oxygen meter shows that the oxygen concentration in the hypoxic chamber is 15%, the vacuum pump stops working, and the experimental animals are continuously hypoxic for 4 hours every day at this oxygen concentration.

[0020] S34. During hypoxia, due to the respiration of the experimental animals, the oxygen concentration in the hypoxic chamber fluctuates. The air exchange hole on the hypoxic chamber can be opened for gas exchange to maintain the oxygen concentration in the hypoxic chamber between 15 ± 0.5%.

[0021] An animal qi - deficiency model constructed according to the above - mentioned method for constructing an animal qi - deficiency model based on the "Chang Sang Jun Pulse Method".

[0022] Application of the animal qi - deficiency model constructed according to the above - mentioned method for constructing an animal qi - deficiency model based on the "Chang Sang Jun Pulse Method" in the research on the treatment mechanism of qi - deficiency - related diseases of the "Chang Sang Jun Pulse Method".

[0023] In the above - mentioned method for constructing an animal qi - deficiency model based on the "Chang Sang Jun Pulse Method", its application, and the animal qi - deficiency model, the normal pulse rate ratio of New Zealand rabbits is about 4.01, approximately equal to 4, which is within the range of the pulse rate ratio of normal people. Therefore, using them as experimental animals, then, the experimental animals are subjected to chronic hypoxia for 12 to 16 days, continuously hypoxic for 3 to 4 hours every day, and the oxygen concentration inhaled by the experimental animals is controlled at 15 ± 0.5% during continuous hypoxia every day to achieve the pathological process of chronic hypoxia, successfully inducing the pulse rate ratio of New Zealand rabbits to be less than 4, indicating that the modeling is successful, and indicating that this method can simulate a specific physiological and pathological state. This method provides a stable experimental platform for the subsequent research on the application of the Chang Sang Jun Pulse Method in this state. This model can be applied to the research on the treatment mechanism of qi - deficiency - related diseases of the "Chang Sang Jun Pulse Method"; the state where the pulse rate ratio presented by this model is less than 4 coincides with the abnormal qi and blood conditions corresponding to certain specific pulse conditions in the Chang Sang Jun Pulse Method. In model rabbits, through traditional pulse detection means combined with modern instrument analysis, it is possible to observe the ratio - characteristic changes consistent with the description of the pulse rate technique of the "Chang Sang Jun Pulse Method", further verifying the operability and scientific nature of the "Chang Sang Jun Pulse Method" in an objective physiological and pathological model; this model provides an opportunity for in - depth research on the internal mechanism of the "Chang Sang Jun Pulse Method", helping to reveal the scientific basis for its judgment of qi and blood circulation from the perspective of modern medicine. For example: by analyzing the correlation between vasoactive substances, hemorheological indexes and pulse characteristics in the hypoxia model, it is expected to explain the microscopic mechanism of the relationship between pulse formation and qi and blood state in the Chang Sang Jun Pulse Method, providing support for the modern development of traditional Chinese medicine pulse theory. Brief Description of the Drawings

[0024] Figure 1 It is a schematic structural diagram of the device in the present invention.

[0025] Figure 2 It is a sectional view of the device in the present invention.

[0026] Figure 3 This is the graph of the pulse - breath ratio change of each group of experimental animals in the first week of conventional feeding in the present invention.

[0027] Figure 4 This is the graph of the pulse - breath ratio change of each group of experimental animals in the first week of modeling in the present invention.

[0028] Figure 5 This is the graph of the pulse - breath ratio change of each group of experimental animals in the second week of modeling in the present invention.

[0029] Figure 6 This is the graph of the pulse - breath ratio change of each group of experimental animals in the first week of treatment in the present invention.

[0030] Figure 7 This is the graph of the pulse - breath ratio change of each group of experimental animals in the second week of treatment in the present invention.

[0031] In the figure, there are water tank box 100, animal platform 110, hypoxia box 200, ventilation hole 210, square tank 230, oxygen concentration detector 300, oxygen concentration adjustment component 400, oxygen delivery part 410, and vacuum pump 420. Detailed implementation manners

[0032] To make the purpose and technical solutions of the present invention clearer, the content of the present invention will be further described in detail through experiments. However, it should not be understood that the scope of the above - mentioned subject matter of the present invention is limited to the following experiments only. Any corresponding replacement or modification made according to the common general knowledge and conventional means in the art without departing from the above - mentioned technical premise of the present invention is included in the present invention.

[0033] The following embodiments are explanations of the present invention, and the present invention is not limited to the following embodiments.

[0034] 1. Experimental materials and methods

[0035] 1.1 Experimental animals

[0036] In this experiment, New Zealand rabbits were used as experimental animals, with a number of 24, half male and half female, and the body weight was 2 kg to 2.5 kg. They were purchased from the Experimental Animal Center of Ningxia Medical University. The experimental animal production license number is: SCXK(Shanghai)2017 - 0002, and the experimental animal use license number is: SYXK(Shanghai)2017 - 0004.

[0037] 1.2 Experimental devices

[0038] This experiment provides a chronic hypoxia animal device with adjustable oxygen concentration, including a water tank box 100 with an open top, an anoxic box 200 with an open bottom, an oxygen concentration detector 300, and an oxygen concentration adjustment component 400. The length and width of the water tank box 100 are respectively greater than those of the anoxic box 200. An animal platform 110 is arranged in the water tank box 100. The anoxic box 200 is covered in the water tank box 100 and covers the animal platform 110 inside. The oxygen concentration detector 300 is arranged in the anoxic box 200 for detecting the oxygen concentration in the anoxic box 200. The oxygen adjustment component includes an oxygen delivery part 410 and a vacuum pump 420. One end of the oxygen delivery part 410 passes through the box body of the anoxic box 200 and is located inside the anoxic box 200. The other end of the oxygen delivery part 410 is connected to the vacuum pump 420. At least one air exchange hole 210 is also opened on the box body of the anoxic box 200, and each air exchange hole 210 is equipped with a rubber soft plug, which can be tightly inserted into the air exchange hole 210. The overall structure diagram of the whole device is as shown in Figure 1 shown, and the cross-sectional view is as shown in Figure 2 shown.

[0039] 1.3 Experimental instruments and reagents

[0040] Strap-type blood oxygen meter (Production License Number: Hunan Food and Drug Administration Production License 20180028);

[0041] Respiratory sensor (Hefei Huake Electronic Technology Research Institute);

[0042] Soda lime (Shanghai Nahui Drying Reagent Factory);

[0043] Vacuum pump 420 (Model: 555 pump);

[0044] The oxygen concentration detector 300 selects an oxygen meter (Henan Province Kailu Electronic Technology Co., Ltd.).

[0045] 1.4 Experimental methods

[0046] The specific method of "pulse and breath technique" is to divide the obtained value by dividing the number of pulse beats per minute by the number of breaths at rest of the patient (pulse / breath). Among them, a pulse-breath ratio greater than 5 is called "pulse restlessness", less than 4 is called "shortness of breath", and 4-5 is called "normal person". In this experiment, the number of pulse beats per minute and the number of breaths at rest of the experimental animals were measured.

[0047] The following experimental data were analyzed and graphed using SPSS 27.0 and Graphpad Prism 10.0 software. The analysis results of each group of data are presented as It is indicated that one-way analysis of variance (ANOVA) is used to compare the results among multiple groups. When P < 0.05, the difference is statistically significant, and when P < 0.01, the difference is highly significant.

[0048] 2. Model establishment

[0049] 2.1 Grouping of experimental animals

[0050] The above-mentioned experimental animals were divided into 4 groups, with 6 animals in each group, half male and half female, namely the control group, the mild hypoxia group, the moderate hypoxia group, and the severe hypoxia group.

[0051] 2.2 Normal feeding of experimental animals one week before modeling

[0052] The New Zealand rabbits in the control group, the mild hypoxia group, the moderate hypoxia group, and the severe hypoxia group were normally fed for 7 days. During the normal feeding period, behavioral observations were carried out on them, and the femoral artery pulse rate of the control group and the hypoxia group of New Zealand rabbits was measured daily using a strap-type blood oxygen meter. The abdominal undulation changes of the control group and the hypoxia group of New Zealand rabbits were measured daily using a respiratory sensor, and the respiratory rate was calculated. Based on the pulse rate and the respiratory rate, the daily pulse-respiration ratio was calculated, and the pulse-respiration ratio was analyzed and graphed. The results are as Figure 3 shown in Table 1:

[0053] Table 1 One-sample T-test table of New Zealand rabbits in each group one week before modeling

[0054]

[0055]

[0056] 2.3 Changes in the pulse-respiration ratio of experimental animals two weeks after modeling

[0057] The control group was normally raised for 14 days. The hypoxia group was modeled using a chronic hypoxia animal device with adjustable oxygen concentration. The number of chronic hypoxia animal devices with adjustable oxygen concentration was 3. First, the side air vent 210 of the hypoxia chamber 200 was sealed with a rubber soft plug. At least one square groove 230 was detachably arranged in the hypoxia chamber 200, and soda lime was placed in the square groove 230. The soda lime was used to absorb the carbon dioxide and water vapor generated by the respiration of New Zealand rabbits during the modeling process. After placing the New Zealand rabbits in each group on the corresponding animal platform 110, the oxygen analyzer was placed into the hypoxia chamber 200, and the oxygen analyzer monitored the oxygen concentration in the corresponding hypoxia chamber 200 in real time, so as to obtain chronic hypoxia animal experimental models with different degrees. Finally, the hypoxia chamber 200 was placed in the water tank 100, and water was poured into the water tank 100 to a pre-calibrated height to achieve the effect of water seal. The vacuum pump 420 could be used to extract part of the air in the hypoxia chamber 200 to quickly reduce the oxygen concentration and gradually reduce it to the ideal state, that is, 20% for the mild hypoxia group, 15% for the moderate hypoxia group, and 10% for the severe hypoxia group. Subsequently, air could be input through the air vent 210, the vacuum pump 420, and the oxygen delivery part 410 to continuously maintain the oxygen concentration in the mild hypoxia chamber 200 at 20±0.5%, the oxygen concentration in the moderate hypoxia chamber 200 at 15±0.5%, and the oxygen concentration in the severe hypoxia chamber 200 at 10±0.5%, so as to realize the pathological process of chronic hypoxia. The New Zealand rabbits in the three hypoxia groups were continuously hypoxic for 4 hours every day for a total of 14 days. During the hypoxia period, their behavior was observed, and the femoral artery pulse rate of the New Zealand rabbits in the control group and the hypoxia groups was measured daily using a strap-type oxygen saturation monitor. The abdominal undulation changes of the New Zealand rabbits in the control group and the hypoxia groups were measured daily using a respiratory sensor, and the respiratory rate was calculated. According to the pulse rate and the respiratory rate, the daily pulse-respiration ratio was calculated, and the pulse-respiration ratio was analyzed and graphed. The specific results are as Figure 4 (during the first week of modeling) and Figure 5 (during the second week of modeling) as shown.

[0058] 2.4 Changes in the pulse-respiration ratio of experimental animals after two weeks of treatment

[0059] After the modeling was completed, the New Zealand rabbits in the above mild hypoxia group, moderate hypoxia group, and severe hypoxia group were treated by acupuncture for two weeks. During the treatment period, their pulse-respiration ratio was detected daily, and it was compared with the daily pulse-respiration ratio of the New Zealand rabbits in the control group. The experimental data of the pulse-respiration ratio of the four groups of New Zealand rabbits during the treatment period were analyzed and graphed. The results are as Figure 6 (during the first week of treatment) and Figure 7 (during the second week of treatment) as shown.

[0060] 3. Experimental results

[0061] 3.1 Results of behavioral observation and analysis

[0062] In the control group, the respiratory rate, depth, and activities of New Zealand rabbits were normal. In the mild hypoxia group, the New Zealand rabbits showed mild restlessness during the modeling process, with rapid or normal breathing, and occasionally huddled together and curled up; in the moderate hypoxia group, there was restlessness, head tremors, rapid breathing, reduced fur gloss, huddling, and curling up; in the severe hypoxia group, there was rapid breathing or slowed and deepened breathing, and gradually piloerection, curling up, and huddling occurred, and finally cyanosis of the lips and sighing respiration appeared.

[0063] 3.2 Detection and analysis results of the pulse-breath ratio in each group

[0064] As Figure 3 and Table 1 show, in the week before the experiment, that is, during the conventional feeding period, the distribution of the pulse-breath ratio of the four groups of New Zealand rabbits basically met the range between 4 and 5, and there was no significant difference (P < 0.05), indicating that the distribution among the groups was uniform and consistent and could meet the experimental criteria; as Figure 4 and the analysis results show, in the first week of modeling, there was a significant difference between the control group and the moderate hypoxia group, with P < 0.05; as Figure 5 and the analysis results show, in the second week of modeling, there was a significant difference between the control group and the moderate hypoxia group, with P < 0.05, and there was also a significant difference between the control group and the mild hypoxia and moderate hypoxia groups, with P < 0.05; among them, the mean difference between the control group and the moderate hypoxia group was greater than that between the control group and the mild hypoxia group, and the effect was more obvious; as Figure 6 and the analysis results show, there were significant differences between the control group and the mild hypoxia group and the moderate hypoxia group (P < 0.05), but the mean difference between the control group and the moderate hypoxia group was larger and the effect was more significant; as Figure 7 and the analysis results show, there were significant differences between the control group and the mild hypoxia group and the moderate hypoxia group (P < 0.05), but the mean difference between the control group and the mild hypoxia group was larger and the effect was more significant.

[0065] Therefore, during both the modeling period and the treatment period, a significant gap was found between the control group and the hypoxia groups (P < 0.05), and the difference mainly existed between the control group and the moderate hypoxia group.

[0066] 3.3 One-sample T-test and comparison of the data in the moderate hypoxia group

[0067] Perform a one-sample T-test on the pulse-breath ratio data of the moderate hypoxia group before the above-mentioned experiment (during the conventional feeding period), 1 week after intervention (the first week of modeling), 2 weeks after intervention (the second week of modeling), 1 week after treatment (the first week of treatment), and 2 weeks after treatment (the second week of treatment) and compare it with the pulse-breath ratio of 4, as shown in Table 2:

[0068] Table 2 Comparison table of one-sample T-test of the data in the moderate hypoxia group and 4

[0069]

[0070] The data in Table 2 indicate that the pulse-breath ratio in the moderate hypoxia group is significantly less than 4 (P < 0.05).

[0071] To sum up, through the measurement of the pulse-breath ratio, the pulse-breath ratio in the mild hypoxia group is not completely less than 4, the pulse-breath ratio in the moderate hypoxia group is less than 4, and both less than 4 and greater than 5 exist in the severe hypoxia group. In this embodiment, moderate hypoxia (oxygen concentration 15 ± 0.5%) is an ideal method for establishing an animal model with qi deficiency, which has the advantages of being more convenient, more time-saving, and more stable, and can be used to construct an animal model with qi deficiency. At the same time, the experimental model in this experiment is highly consistent with the qi deficiency manifestation of the "Chang Sang Jun Pulse Method". Therefore, chronic moderate hypoxia can be used as the basic method for establishing the qi deficiency type of the "Chang Sang Jun Pulse Method".

[0072] In the above method for establishing an animal model with qi deficiency based on the "Chang Sang Jun Pulse Method", its application, and the animal model with qi deficiency, the normal pulse-breath ratio of New Zealand rabbits is about 4.01, approximately equal to 4, which is within the pulse-breath ratio range of normal people. Therefore, New Zealand rabbits are used as experimental animals, and then the experimental animals are subjected to chronic hypoxia for 12 to 16 days, with continuous hypoxia for 3 to 4 hours every day. When continuously hypoxic every day, the oxygen concentration inhaled by the experimental animals is controlled at 15 ± 0.5% to achieve the pathological process of chronic hypoxia, successfully inducing the pulse-breath ratio of New Zealand rabbits to be less than 4, indicating that the modeling is successful, and indicating that this method can simulate a specific physiological and pathological state. This method provides a stable experimental platform for subsequent research on the application of the Chang Sang Jun Pulse Method in this state. This model can be applied to the research on the treatment mechanism of diseases related to qi deficiency of the "Chang Sang Jun Pulse Method". The state where the pulse-breath ratio presented by this model is less than 4 conforms to the abnormal qi and blood conditions corresponding to certain specific pulse conditions in the Chang Sang Jun Pulse Method. In model rabbits, through traditional pulse detection methods combined with modern instrument analysis, it is possible to observe the ratio characteristic changes consistent with the description of the pulse-breath technique of the "Chang Sang Jun Pulse Method", further verifying the operability and scientific nature of the "Chang Sang Jun Pulse Method" in an objective physiological and pathological model. This model provides an opportunity for in-depth research on the internal mechanism of the "Chang Sang Jun Pulse Method", and helps to reveal the scientific basis for its judgment of qi and blood circulation from the perspective of modern medicine. For example, by analyzing the correlation between vasoactive substances, hemorheological indexes and pulse characteristics in the hypoxia model, it is expected to explain the microscopic mechanism of the relationship between pulse formation and qi and blood state in the Chang Sang Jun Pulse Method, providing support for the modern development of traditional Chinese medicine pulse theory.

[0073] As described above, the above is only the specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention can easily think of various equivalent modifications or substitutions, and these modifications or substitutions should all be covered within the protection scope of the present invention.

Claims

1. A method for constructing an animal model of qi deficiency based on the "Pulse Diagnosis Method of Chang Sang Jun", characterized in that, It includes the following steps: S10. Select experimental animals, and the normal pulse-breath ratio of the experimental animals is basically the same as that of normal humans. S20. Raise the experimental animals conventionally, and detect their pulse rate and respiratory rate daily during the conventional breeding period, calculate the daily pulse-breath ratio, and the daily pulse-breath ratio is between 4 and 5. S30. After the conventional breeding ends, subject the experimental animals to chronic hypoxia for 12 to 16 days, with continuous hypoxia for 3 to 4 hours per day. During the continuous hypoxia per day, control the oxygen concentration inhaled by the experimental animals to be 15±0.5%, to achieve the pathological process of chronic hypoxia. During chronic hypoxia, detect their pulse rate and respiratory rate daily, calculate the daily pulse-breath ratio. If the daily pulse-breath ratio is less than 4 every day, it indicates that the modeling is successful.

2. The method for constructing an animal model of qi deficiency based on the "Chang Sang Jun Pulse Method" according to claim 1, wherein The experimental animals are of the rabbit breed.

3. The method for constructing an animal model with qi deficiency based on the "Chang Sang Jun Pulse Method" according to claim 1, characterized in that, The experimental animals are New Zealand rabbits.

4. The method for constructing an animal model of qi deficiency based on the "Chang Sang Jun Pulse Method" according to claim 1, wherein, Raise the experimental animals conventionally for 5 to 10 days.

5. The method for constructing an animal model with qi deficiency based on the "Chang Sang Jun Pulse Method" according to claim 1, characterized in that, Subject the experimental animals to chronic hypoxia for 14 days, with continuous hypoxia for 4 hours per day.

6. The method for constructing an animal model of qi deficiency based on the "Chang Sang Jun Pulse Method" according to claim 1, wherein The chronic hypoxia experiment device with adjustable oxygen concentration can be used to subject the experimental animals to chronic hypoxia. The chronic hypoxia experiment device with adjustable oxygen concentration includes: a water tank box with an open top, a hypoxia box with an open bottom, an oxygen concentration detector, and an oxygen concentration adjustment component. The length and width of the water tank box are respectively greater than the length and width of the hypoxia box. An animal platform is arranged in the water tank box. The hypoxia box is covered in the water tank box and covers the animal platform inside. The oxygen concentration detector is arranged in the hypoxia box for detecting the oxygen concentration in the hypoxia box. The oxygen adjustment component includes an oxygen delivery piece and a vacuum pump. One end of the oxygen delivery piece passes through the box body of the hypoxia box and is located inside the hypoxia box, and the other end of the oxygen delivery piece is connected to the vacuum pump. At least one air exchange hole is also opened on the box body of the hypoxia box, and each air exchange hole is equipped with a rubber soft plug, and the rubber soft plug can be tightly inserted into the air exchange hole.

7. The method for constructing an animal model with qi deficiency based on the "Chang Sang Jun Pulse Method" according to claim 6, characterized in that, The specific steps of S30 include the following: S31. Place the experimental animals on the animal platform. S32. Place the hypoxia box in the water tank box, add water to a certain height in the water tank box, and seal the hypoxia box by the water seal method. S33. To quickly reduce the oxygen concentration in the hypoxia box, use the vacuum pump and the delivery piece to extract part of the air in the hypoxia box. When the oxygen concentration detector shows that the oxygen concentration in the hypoxia box is 15%, the vacuum pump stops working, and the experimental animals are continuously hypoxic for 4 hours per day at this oxygen concentration. S34. During hypoxia, due to the respiration of the experimental animals, the oxygen concentration in the hypoxia box fluctuates. The air exchange hole on the hypoxia box can be opened for gas exchange to keep the oxygen concentration in the hypoxia box between 15±0.5%.

8. An animal hypoqi model constructed by the method for constructing an animal hypoqi model based on the "Pulse Method of Chang Sang Jun" according to any one of claims 1 to 7.

9. Use of the animal qi deficiency model constructed by the method for constructing an animal qi deficiency model based on the "Chang Sang Jun Pulse Method" according to any one of claims 1 to 7 in the research on the treatment mechanism of diseases related to qi deficiency by the "Chang Sang Jun Pulse Method".

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