A flow cytometry antibody premixing device

Abstract

The present invention relates to the field of cell detection technology, and in particular to a flow cytometry antibody premixing device. It comprises a box body with a chamber, an elastic movable part extending into the chamber and connecting with the box body, and two notches and gaps are provided on the side wall of the elastic movable part to form two movable flaps. When there is no external force squeezing, the two movable flaps tend to extend and fit toward the notches and gaps to form a closed space in the chamber below the movable flaps; an annular body is installed on the inner wall of the elastic movable part, and when there is external force squeezing, the annular body is deformed to form a closed space in the chamber below the annular body; an EP tube is installed in the chamber for premixing flow cytometry antibodies. The present invention can ensure that the EP tube in the box body is always in a closed state isolated from the outside world during the process of configuring the antibody mixture, thereby reducing contact with the outside world and reducing the risk of contamination of the antibody mixture.

Description

Technical Field

[0001] The present invention relates to the technical field of cell detection, in particular to a flow cell antibody premixing device. Background Art

[0002] Flow cytometry is a single-cell quantitative analysis and sorting technology that uses a flow cytometer. It can perform multi-parameter high-speed analysis and sorting of small particles such as cells and microorganisms. It is quantitative, flexible, and fast, and is one of the most cutting-edge cell quantitative analysis technologies at this stage.

[0003] Some specific reagents are very important for flow cytometry, such as fluorescent dye-labeled antibodies against specific cell antigens. Testers need to optimize each protocol to increase accuracy and reduce errors and differences, and ultimately determine the best available antibodies, fluorophores, and their combinations. Antibody mixtures are very useful tools in clinical process laboratories and contribute to the consistency of detection. Antibody mixtures are single antibodies for analysis combined into one tube. Compared with direct purchase, testers prepare antibody mixtures with the advantages of low cost and flexible customization. When preparing antibody mixtures, each monoclonal antibody must be titrated separately to achieve the best signal-to-noise ratio separation.

[0004] When a large number of antibodies need to be mixed to prepare the antibody mixture, the EP tube for preparing the antibody mixture will remain in a continuously open state. When the tester is performing the preparation operation, the continuously open state of the EP tube will increase the risk of contamination of the antibody mixture. Summary of the Invention

[0005] The present invention aims to at least improve one of the technical problems existing in the prior art. To this end, the present invention provides a flow cytometry antibody premixing device.

[0006] The technical solutions of the present invention are as follows:

[0007] A flow cytometry antibody premixing device, comprising:

[0008] The box body has a chamber, and a through hole is opened on the surface of the box body;

[0009] an elastic movable member passing through the through hole and extending into the chamber, the elastic movable member being engaged with the surface of the box body, wherein the diameter of the elastic movable member away from the surface of the box body gradually shrinks so as to allow the pipette to pass through and extend into the chamber, and two notches are provided on the side wall of the elastic movable member to form two movable flaps, and when there is no external force, the two movable flaps tend to extend and fit toward the notches to form a closed space in the chamber below the movable flaps;

[0010] an annular body, mounted on the inner wall of the elastic movable member and located above the movable petal, wherein when an external force is applied, the annular body is deformed so that the chamber below the annular body forms a closed space;

[0011] The EP tube is installed in the chamber and is used for pre-mixing flow cytometry antibodies.

[0012] In a possible technical solution, further, the annular body includes:

[0013] A plurality of limiting bars are distributed in an annular pattern with intervals, and a gap is formed between adjacent limiting bars;

[0014] A closed belt, each of which passes through the gap and connects two adjacent limit strips to form an annular closed loop, is used to deform the annular body when external force is applied so that the chamber below the annular body forms a closed space.

[0015] In a possible technical solution, further, the closing band has an arc-shaped profile that deviates from the limiting strip. When squeezed by external force, the closing band stretches toward the limiting strip to increase the area enclosed by the annular body, thereby providing displacement space for the pipette gun.

[0016] In a possible technical solution, further, the annular body further includes:

[0017] One end of the clamping piece is connected to the side wall of the limiting strip that deviates from the elastic movable piece.

[0018] In a possible technical solution, further, the closing band has a first side arc and a second side arc, wherein the first side arc is an inner side arc close to the surface of the limiting strip, and the second side arc is an outer side arc deviated from the surface of the limiting strip, and the arc length of the first side arc is greater than the arc length of the second side arc.

[0019] In a possible technical solution, further, the end of the card member away from the limit strip is arranged close to the inner wall of the elastic movable part, and the card member and the upper surface of the limit strip and the inner wall of the elastic movable part form a card gap for the closing belt to pass through, thereby improving the overall airtightness of the device, wherein the card member is used to increase the limiting effect of the card gap.

[0020] In a possible technical solution, further, the closing belt is made of elastic material to facilitate expansion and closing of the gap under pressure.

[0021] In a possible technical solution, further, the two movable petals are symmetrically distributed, so as to facilitate uniform outward support when under pressure to ensure that the pipette moves vertically downward, and avoid asymmetric deformation causing the pipette to touch the movable petals.

[0022] In a possible technical solution, further, a groove is provided inside the box body for fixing the EP tube.

[0023] In a possible technical solution, further, any side of the box is provided with a visual glass for easy observation.

[0024] The flow cytometry antibody premixing device of the present invention can ensure that the EP tube containing the antibody mixture in the box is in a closed state isolated from the outside world throughout the entire process before and after the antibody is prepared, thereby reducing contact with the outside world and lowering the risk of contamination of the antibody mixture. This is specifically reflected in:

[0025] At least two movable petals are formed by providing at least two notches at the lower end of the elastic movable member. When the pipette enters the elastic movable member, a supporting force is generated between the surface of the pipette nozzle ejector and the inner wall of the limiting strip, and the movable petals are pushed outward, making it easier for the nozzle ejector and the disposable nozzle cone to enter the cavity of the box body. In addition, due to the limiting effect of the clamping member on the closing band, the closing band maintains a tight closed fan ring gap during the process of passing through the fan ring gap between the limiting strips, thereby isolating the closing band from external contact.

[0026] During the opening process of the lower end of the movable flap, the lower end of the cavity of the elastic movable part surrounded by the movable flap is connected with the cavity of the box body, so that the whole formed by at least two limit strips is a complete annular body under the penetration of the closing belt, which is convenient for separation from the upper end of the cavity of the elastic movable part, ensuring the sealing of the entire pipette gun when entering the device during antibody mixing, reducing the risk of contamination of the antibody mixture due to the continuous opening of the EP tube.

[0027] Additional aspects and advantages of the present invention will be set forth in part in the description which follows and, in part, will be obvious from the description which follows, or may be learned by practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0029] Figure 1 is an overall schematic diagram of a flow cytometry antibody premixing device according to an embodiment of the present invention;

[0030] Figure 2 2. Schematic diagram of the opening and closing door of the flow cytometry antibody premixing device according to an embodiment of the present invention;

[0031] Figure 32. It is a schematic structural diagram of an elastic movable member of a flow cytometry antibody premixing device according to an embodiment of the present invention;

[0032] Figure 4 2. This is a schematic structural diagram of an elastic movable member of a flow cytometry antibody premixing device according to an embodiment of the present invention from another perspective;

[0033] Figure 5 2. It is a schematic diagram of the annular structure of the flow cytometry antibody premixing device according to an embodiment of the present invention;

[0034] Figure 6 is an overall cross-sectional view of the flow cytometry antibody pre-mixing device before deformation according to an embodiment of the present invention;

[0035] Figure 7 is a partial enlarged view of the flow cytometry antibody pre-mixing device before deformation according to an embodiment of the present invention;

[0036] Figure 8 is a deformed internal cross-sectional view of a flow cytometry antibody premixing device according to an embodiment of the present invention;

[0037] Figure 9 is a partially enlarged cross-sectional view of a deformed flow cytometry antibody premixing device according to an embodiment of the present invention;

[0038] Figure 10 2 is a schematic diagram of the deformation of the annular body of the flow cytometry antibody pre-mixing device before and after being compressed according to an embodiment of the present invention;

[0039] Figure 11 2. It is a planar cross-sectional view of the lower and upper ends of the limiting bar of the flow cytometry antibody premixing device according to an embodiment of the present invention;

[0040] Figure 12 3. This is a comparison diagram of the overall details of the limiting strips and gaps of the flow cytometry antibody pre-mixing device before and after deformation according to an embodiment of the present invention;

[0041] Figure 13 1 is a detailed diagram of the limiting strips and the positioning gaps of the flow cytometry antibody premixing device according to an embodiment of the present invention.

[0042] Reference numerals:

[0043] Box 1, chamber 101, groove 102, sight glass 103, switch door 104;

[0044] Elastic movable part 2, notch gap 201, movable petal 202;

[0045] Pipette 3, nozzle ejector 31, disposable nozzle cone 32;

[0046] annulus 4;

[0047] EP tube 5:

[0048] Limiting strip 41 , gap 410 , closing strip 42 , clamping piece 43 , and clamping gap 430 . DETAILED DESCRIPTION

[0049] The embodiments of the present invention are described in detail below. The embodiments described with reference to the accompanying drawings are exemplary. It should be understood that the specific embodiments described herein are only used to explain the present application and are not used to limit the present application.

[0050] It should be noted that when an element is referred to as being “fixed to” another element, it may be directly on the other element or there may be an intermediate element. When an element is referred to as being “connected to” another element, it may be directly connected to the other element or there may be an intermediate element.

[0051] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this invention pertains. The terms used in this specification of the present invention are for the purpose of describing specific embodiments only and are not intended to limit the present invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0052] In the specification, claims, and accompanying drawings of this application, the terms "first," "second," "third," and the like are used to distinguish different objects and are not used to describe a particular order. Furthermore, the terms "including," "comprising," "having," and any variations thereof are intended to cover non-exclusive inclusions. For example, a list of steps or elements may be included, or alternatively, steps or elements not listed may be included, or other steps or elements may be included that are inherent to the process, method, product, or apparatus.

[0053] Only portions relevant to the present application are shown in the accompanying drawings, not all of them. Before discussing the exemplary embodiments in more detail, it should be noted that some exemplary embodiments are described as processes or methods depicted as flow charts. Although the flow charts describe the various operations (or steps) as sequential processes, many of the operations can be performed in parallel, concurrently, or simultaneously. In addition, the order of the various operations can be rearranged. The process can be terminated when its operations are completed, but can also have additional steps not included in the accompanying drawings. The process can correspond to a method, function, procedure, subroutine, subprogram, etc.

[0054] As used in this specification, the terms "component," "module," "system," "unit," and the like are used to refer to computer-related entities, hardware, firmware, a combination of hardware and software, software, or software in execution. For example, a unit can be, but is not limited to, a process running on a processor, a processor, an object, an executable file, an execution thread, a program, and / or distributed between two or more computers. In addition, these units can be executed from various computer-readable media having various data structures stored thereon. Units can communicate, for example, via local and / or remote processes based on signals having one or more data packets (e.g., data from a second unit interacting with another unit in a local system, a distributed system, and / or a network, such as the Internet, which interacts with other systems via signals).

[0055] Example 1

[0056] like Figures 1 to 13 As shown, this embodiment provides a flow cytometry antibody premixing device, which includes:

[0057] The box body 1 is arranged vertically and has a chamber 101. A through hole is opened on the surface of the box body 1.

[0058] The elastic movable part 2 passes through the through hole and extends into the chamber 101. The elastic movable part 2 is connected to the surface of the box body 1, wherein the diameter of the elastic movable part 2 away from the surface of the box body 1 gradually shrinks for the pipette 3 to pass through and extend into the chamber 101. The side wall of the elastic movable part 2 is provided with two notches 201 to form two movable petals 202. When there is no external force, under the elastic action of the elastic movable part 2, the two movable petals 202 tend to extend and fit toward the notches 201 so that the chamber 101 below the movable petals 202 forms a closed space. When subjected to external force, the two movable petals 202 gradually expand in a direction deviating from each other, and the lower ends of the two movable petals 202 are separated from the tightly attached state. When the disposable nozzle cone 32 of the pipette 3 completely enters the chamber 101, the disposable nozzle cone 32 is limited by the lower ends of the two movable petals 202.

[0059] The annular body 4 is mounted on the inner wall of the elastic movable member 2 and is located above the movable petal 202. When an external force is applied, the annular body 4 is deformed so that the chamber 101 below the annular body 4 forms a closed space.

[0060] The EP tube 5 is installed in the chamber 101 and is used for pre-mixing flow cytometry antibodies.

[0061] It should be noted that the elastic movable part 2 is a semi-closed hollow cone structure as a whole, and its upper surface has an opening for communicating with the outside world, wherein the diameter of the elastic movable part 2 away from the surface of the box body 1 gradually shrinks, specifically, the area S1 enclosed by the upper edge of the elastic movable part 2 is larger than the overall area S2 formed by the lower end face of the elastic movable part 2.

[0062] It should be noted that, in this embodiment, the pipette 3 is a universal pipette, comprising a nozzle ejector 31 and a disposable nozzle cone 32 , wherein the disposable nozzle cone 32 is mounted at the bottom end of the nozzle ejector 31 .

[0063] It should be noted that, in this embodiment, the annular body 4 includes:

[0064] A plurality of limiting strips 41 are distributed in an annular pattern, with a gap 410 between adjacent limiting strips 41. In this embodiment, the gap 410 is a fan-shaped gap.

[0065] The closed belt 42 passes through the gap 410 and connects two adjacent limiting strips 41 to form an annular closed loop. When external force is applied, the annular body 4 is deformed so that the chamber 101 below the annular body 4 forms a closed space.

[0066] It should be noted that, in this embodiment, the closing strip 42 has an arc-shaped profile that deviates from the limiting strip 41. Figure 12 (a) is a schematic diagram of the limit strip before it is deformed by pressure. When external force is applied, the closing band 42 stretches toward the limit strip 41 to increase the area enclosed by the annular body 4, providing displacement space for the pipette 3. Figure 12 (b) is a schematic diagram showing the expansion of the gap after the limit bar is deformed under pressure.

[0067] It should be noted that, in this embodiment, the closing band 42 has a first side arc L1 and a second side arc L2, wherein the first side arc L1 is an inner side arc close to the surface of the limiting strip 41, and the second side arc L2 is an outer side arc deviated from the surface of the limiting strip 41, and the arc length of the first side arc L1 is greater than the arc length of the second side arc L2.

[0068] like Figure 10 As shown, Figure 10 (a) is a schematic diagram of the annular body before being compressed. When no deformation occurs, the area enclosed by the inner edge of the entirety formed by the limiting strip 41 is P1, and the inner surface of the entirety formed by the limiting strip 41 is in contact with the lower end surface of the nozzle ejector 31 of the pipette 3; Figure 10As shown in (b), it is a schematic diagram of the deformation of the annular body after being compressed. In the fully deformed state, the area enclosed by the integral inner edge of the limiting strip 41 formed by extrusion is P2, and the cross-sectional area of ​​the upper end of the nozzle ejector is S3, satisfying S1>S3≥P2>P1; the cavity of the elastic movable part surrounded by the movable petal 202 can accommodate the disposable nozzle cone 32 of the pipette gun 3.

[0069] It should be noted that the closing strips 42 are made of elastic material so as to be expanded when under pressure to close the gaps 410 between the limiting strips 41 .

[0070] It should be noted that, in this embodiment, the annular body 4 further includes:

[0071] One end of the latch 43 engages with the side wall of the limiting strip 41, which is offset from the elastic movable member 2. The end of the latch 43, which is away from the limiting strip 41, is positioned closer to the inner wall of the elastic movable member 2, i.e., the latch 43 as a whole radiates outward. The latch 43, the upper surface of the limiting strip 41, and the inner wall of the elastic movable member 2 define a retaining gap 430 for the closure band 42 to pass through, thereby enhancing the overall airtightness of the device. The latch 43 is used to enhance the retaining effect of the retaining gap 430.

[0072] It should be noted that, in this embodiment, the two movable petals 202 are symmetrically distributed, so as to evenly support the pipette 3 when under pressure to ensure that the pipette 3 moves vertically downward, and avoid asymmetric deformation causing the pipette 3 to touch the movable petals 202.

[0073] It should be noted that, in this embodiment, a groove 102 is provided inside the box 1 for fixing the EP tube 5 so that the disposable nozzle cone 32 containing the antibody can be inserted into the EP tube 5. The elastic movable part 2 is located directly above the groove 102, which facilitates the pre-mixing of cells and antibodies by the pipette.

[0074] The flow cytometry antibody premixing device of the present invention can ensure that the EP tube containing the antibody mixture in the box is in a closed state isolated from the outside world throughout the entire process before and after the antibody is prepared, thereby reducing contact with the outside world and lowering the risk of contamination of the antibody mixture. This is specifically reflected in:

[0075] At least two movable petals are formed by providing at least two notches at the lower end of the elastic movable member. When the pipette enters the elastic movable member, a supporting force is generated between the surface of the pipette nozzle ejector and the inner wall of the limiting strip, and the movable petals are pushed outward, making it easier for the nozzle ejector and the disposable nozzle cone to enter the cavity of the box body. In addition, due to the limiting effect of the clamping member on the closing band, the closing band maintains a tight closed fan ring gap during the process of passing through the fan ring gap between the limiting strips, thereby isolating the closing band from external contact.

[0076] During the opening process of the lower end of the movable flap, the lower end of the cavity of the elastic movable part surrounded by the movable flap is connected with the cavity of the box body, so that the whole formed by at least two limit strips is a complete annular body under the penetration of the closing belt, which is convenient for separation from the upper end of the cavity of the elastic movable part, ensuring the sealing of the entire pipette gun when entering the device during antibody mixing, reducing the risk of contamination of the antibody mixture due to the continuous opening of the EP tube.

[0077] Example 2

[0078] This embodiment makes further improvements on the basis of embodiment 1, and provides a flow cytometry antibody premixing device, wherein:

[0079] At least one side of the box body 1 is provided with a visual glass 103 to facilitate observation of the operation process.

[0080] A switch door 104 is provided on one side of the box 1 to facilitate the placement of the EP tube 5 into the box 1. A sealing ring is provided at the connection between the switch door 104 and the box 1 to improve the airtightness of the chamber 101 and reduce the risk of contamination of the antibody mixture.

[0081] This embodiment provides a flow cytometry antibody premixing method, comprising the following steps:

[0082] S1, pre-mixing preparation: open the EP tube cover, hold the EP tube body with tweezers, put it into the groove in the box through the switch door, and close the switch door;

[0083] S2, aspirate antibodies: Install the disposable nozzle cone to the lower end of the pipette nozzle pusher, adjust the pipette aspiration capacity, and aspirate flow cytometry antibodies;

[0084] S3, sealed pipetting: Move the pipette gun vertically downward from the upper end of the elastic movable part. When the disposable nozzle cone moves to the lower end of the cavity of the elastic movable part, the surface of the nozzle pusher is attached to the inner wall of the limit strip. Figure 11 As shown in (a), it is a cross-sectional view of the lower end of the limit bar. As the pipette moves downward, the diameter of the pipette nozzle ejector in contact with the inner wall of the limit bar gradually increases, and the limit bar is squeezed, resulting in an increase in the gap between the fan rings, thereby increasing the area enclosed by the limit bar. Figure 11(b) is a planar cross-sectional view of the upper end of the limiting strip, which is squeezed to isolate the chamber from the external environment, forming a closed environment in the chamber. Due to the limiting effect of the positioning gap, in the process of the fan ring gap increasing, one end of the closing belt changes from vertically penetrating the fan ring gap to obliquely penetrating the fan ring gap, and the closing belt is stressed as a whole in a straightened state to close the fan ring gap. As the area enclosed by the two limiting strips increases, the movable flaps are driven to gradually expand outward, and the lower ends of the two movable flaps are separated from the tightly attached state. When the movable flaps are fully expanded, the upper end of the nozzle tube ejector is squeezed and limited by the limiting strip, and the disposable nozzle cone and the lower and middle ends of the nozzle tube ejector enter the sealed chamber, pushing the antibody in the disposable nozzle cone into the EP tube along the wall of the EP tube;

[0085] S4, the pipette exits after pipetting: the pipette is moved vertically upward as a whole until the nozzle ejector is no longer squeezed and limited by the lower ends of the two movable flaps. Due to the squeezing limit bar at the middle end of the nozzle ejector, the lower end of the movable flap is in a separated state, which is conducive to the disposable nozzle cone moving from the chamber to the lower end of the cavity of the elastic movable part. When the nozzle ejector is gradually withdrawn, in the absence of external force, the sealing band and the movable flap are reset by the elastic action of the elastic movable part. The two movable flaps tend to extend and fit toward the notch gap to form a closed space in the chamber below the movable flap. Finally, the pipette is removed from the elastic movable part to complete one pipetting;

[0086] S5, repeat pipetting: Repeat steps S2-S4, add the antibodies to be mixed into the EP tube in sequence, until the last antibody is pushed into the EP tube, repeatedly pump the pipette to mix the antibodies until they are completely mixed;

[0087] S6, mixed sample collection: open the switch door, use tweezers to cover the tube cap of the EP tube lightly on the tube mouth of the EP tube to prevent spillage when taking it out, clamp the top of the EP tube with tweezers, take the EP tube containing the antibody mixture out of the groove in the box, cover the tube cap tightly and set aside.

[0088] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like to indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore should not be understood as a limitation to the invention.

[0089] Throughout this specification, reference to terms such as "one embodiment," "some embodiments," "illustrative embodiments," "example," "specific example," or "some examples" means that a specific feature, structure, material, or characteristic described in conjunction with the embodiment or example is included in at least one embodiment or example of the present invention. In this specification, illustrative expressions of the above terms do not necessarily refer to the same embodiment or example.

[0090] Obviously, the described embodiments are only some of the embodiments of the present application, rather than all of the embodiments. Mentioning "embodiment" in this article means that the specific features, structures or characteristics described in conjunction with the embodiment may be included in at least one embodiment of the present embodiment application. The appearance of this phrase in various positions in the specification does not necessarily mean that they are all the same embodiments, nor are they independent or alternative embodiments that are mutually exclusive with other embodiments. It can be understood explicitly and implicitly by those skilled in the art that the embodiments described herein can be combined with other embodiments. Based on the embodiments in this application, all other embodiments obtained by those of ordinary skill in the art without making creative work are within the scope of protection of this application.

[0091] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to the embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the claims and their equivalents.

Claims

1. A flow cytometry antibody premixing device, characterized in that: include: The box body (1) has a chamber (101), and a through hole is opened on the surface of the box body (1); An elastic movable part (2) passes through the through hole and extends into the chamber (101), the elastic movable part (2) is connected to the box body (1), wherein the diameter of the elastic movable part (2) away from the box body (1) gradually shrinks, so that the pipette gun (3) passes through and extends into the chamber (101), and two notches (201) are provided on the side wall of the elastic movable part (2) to form two movable flaps (202). When there is no external force squeezing, the two movable flaps (202) tend to extend and fit toward the notches (201) so that the chamber (101) below the movable flaps (202) forms a closed space; An annular body (4) is mounted on the inner wall of the elastic movable member (2) and is located above the movable petal (202). When an external force is applied, the annular body (4) is deformed so that the chamber (101) below the annular body (4) forms a closed space. The EP tube (5) is installed in the chamber (101) and is used for pre-mixing flow cytometry antibodies.

2. The flow cytometry antibody premixing device according to claim 1, characterized in that: The annular body (4) comprises: A plurality of limiting strips (41) are distributed in an annular manner, with a gap (410) between adjacent limiting strips (41); A closed belt (42), each closed belt (42) passes through the gap (410) and connects two adjacent limiting strips (41) to form an annular closed loop.

3. The flow cytometry antibody premixing device according to claim 2, characterized in that: The closing strip (42) has an arc-shaped profile that deviates from the limiting strip (41).

4. The flow cytometry antibody premixing device according to claim 2, characterized in that: The annular body (4) further comprises: One end of the clamping member (43) is connected to the side wall of the limiting strip (41) that deviates from the elastic movable member (2).

5. The flow cytometry antibody premixing device according to claim 3, characterized in that: The closing strip (42) has a first side arc and a second side arc, wherein the first side arc is an inner side arc close to the surface of the limiting strip (41), and the second side arc is an outer side arc deviated from the surface of the limiting strip (41), and the arc length of the first side arc is greater than the arc length of the second side arc.

6. The flow cytometry antibody premixing device according to claim 4, characterized in that: The end of the clamping member (43) away from the limiting strip (41) approaches the inner wall of the elastic movable member (2).

7. The flow cytometry antibody premixing device according to claim 5, characterized in that: The closing band (42) is made of elastic material.

8. The flow cytometry antibody premixing device according to claim 1, characterized in that: The two movable petals (202) are symmetrically distributed.

9. The flow cytometry antibody premixing device according to claim 1, characterized in that: A groove (102) is provided on the inner side of the box body (1).

10. The flow cytometry antibody premixing device according to claim 9, characterized in that: A visual glass (103) is provided on any side of the box (1).

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