A stress-resistant and growth-promoting biological organic fertilizer and its preparation method and application

Abstract

The present invention discloses a stress-resistant and growth-promoting bio-organic fertilizer, a preparation method thereof, and an application thereof, and belongs to the technical field of bio-organic fertilizers. The bio-organic fertilizer of the present invention is prepared from microbial agents, potassium fulvic acid, wheat straw, organic acid, mushroom residue, dry sheep manure, corn cobs, urea, fly ash, a synergist, and hawthorn extract. The bio-organic fertilizer of the present invention uses hawthorn extract, compound microbial agents, synergist, and other ingredients, and multiple substances work synergistically to effectively reduce soil salinity and significantly improve soil fertility. At the same time, it improves the stress resistance of corn plants, promotes corn plant growth, increases corn yield, and improves planting efficiency.

Description

Technical Field

[0001] The invention belongs to the technical field of bio-organic fertilizers, and in particular relates to a stress-resistant and growth-promoting bio-organic fertilizer, a preparation method thereof, and an application thereof. Background Art

[0002] Salt-alkali land is a significant reserve land resource in my country, characterized by its large area and widespread distribution. Coastal salt-alkali land has high soil pH and salinity, low porosity, poor soil structure, and reduced water-holding capacity. This affects the conversion of organic matter, reduces soil fertility, and limits plant nutrient absorption, resulting in a waste of agricultural resources and severe economic losses and secondary damage.

[0003] Bio-organic fertilizer combines the benefits of traditional organic fertilizers with those of microbial fertilizers. It contains a large amount of organic matter and beneficial soil bacteria. When bio-organic fertilizer is applied to the soil through deep tillage, it, under the influence of soil microorganisms and enzyme activity, produces large amounts of organic acids, which can lower the soil pH. Beneficial bacteria produce numerous metabolites during their growth and reproduction, promoting the decomposition and transformation of organic matter. These metabolites directly or indirectly provide a variety of nutrients and stimulants to crops, promoting and regulating crop growth. Increasing soil organic matter not only improves soil physical and chemical properties but also enhances its water and fertilizer retention capacity. Soil conditioners, made primarily from natural minerals through high-temperature calcination and extraction, have the potential to improve soil quality, enhance crop resistance to disease, increase crop yields, improve agricultural product quality, and restore the original ecological environment of crops. Bio-organic fertilizers can improve soil physical and chemical properties, but studies have primarily focused on the effects of single organic materials on saline-alkali land. Currently, there is a lack of research specifically examining the effects of bio-organic fertilizers on crop growth and soil physical and chemical properties in coastal saline-alkali land. Summary of the Invention

[0004] The purpose of the present invention is to provide a stress-resistant and growth-promoting bio-organic fertilizer that effectively reduces soil salinity and alkalinity, significantly improves soil fertility, and at the same time improves the stress resistance of crops, promotes crop growth, increases crop yield, and improves crop planting efficiency.

[0005] In order to achieve the above technical objectives, the technical solution adopted by the present invention is:

[0006] A stress-resistant and growth-promoting bio-organic fertilizer is prepared by including the following raw materials in parts by weight: 12-18 parts of microbial agent, 10-15 parts of potassium humate, 75-90 parts of wheat straw, 5-10 parts of organic acid, 45-55 parts of mushroom residue, 28-36 parts of dry sheep manure, 16-22 parts of corn cobs, 0.5-1 part of urea, 3-5 parts of fly ash, 8-10 parts of synergist, and 5-8 parts of hawthorn extract.

[0007] Furthermore, the microbial agent is Bradyrhizobium of the Canary Islands, Bacillus megaterium, and Pseudomonas chengduensis in a mass ratio of 1:1:1.

[0008] Furthermore, the Canary Islands Bradyrhizobium was purchased from the China General Microorganism Collection Center, located at No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, with a deposit number of CGMCC No. 1.15558 and an original deposit date of December 30, 2015; the megaterium was purchased from the China General Microorganism Collection Center, located at No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, with a deposit number of CGMCC No. 1.10466 and an original deposit date of March 18, 2010; the Chengdu Pseudomonas was purchased from the China General Microorganism Collection Center, located at No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, with a deposit number of CGMCC No. 1.15626 and an original deposit date of February 25, 2015.

[0009] Furthermore, the organic acid is citric acid and malic acid in a mass ratio of 1:1.

[0010] Furthermore, the organic acid is diluted 5 times before use.

[0011] Furthermore, the synergist includes 5 parts of potassium nitrate, 2 parts of catechins, and 3 parts of alginic acid.

[0012] A method for preparing stress-resistant and growth-promoting bio-organic fertilizer comprises the following steps:

[0013] (1) Preparation of microbial agents:

[0014] After activation, Bradyrhizobium Canariae, Pseudomonas Chengdui, and Bacillus megaterium were inoculated into LB liquid medium at 28°C, 30°C, and 30°C respectively until OD 600 =0.6 to obtain seed solution, 1% of the Canary Islands Bradyrhizobium seed solution was inoculated into nitrogen-fixing medium, and 1% of Chengdu Pseudomonas and Bacillus megaterium were inoculated into LB liquid medium respectively, and cultured in the medium until the number of viable bacteria reached 1×10 9 cfu·mL -1 obtaining three bacterial solutions, then uniformly mixing the three bacterial solutions in a mass ratio of 1:1:1, and freeze-drying them into freeze-dried powder to obtain a microbial agent;

[0015] (2) Fermentation and composting:

[0016] The mushroom residue, corn cobs, and dry sheep manure are crushed and passed through a 50-mesh sieve, mixed evenly, placed in a fermentation tank, and water is added and mixed evenly to control the moisture content between 50% and 60%. Urea is then placed in the fermentation tank and stirred evenly. The fermentation tank is sealed with a plastic film. The fermentation process is completed once every 7 days. After 3 turnings, the fermentation is completed to obtain fermented organic matter.

[0017] (3) Preparation of compound biochar:

[0018] The wheat straw is dried and crushed to 2 cm, added to a reactor, and carbonized at 300-500°C for 2 hours without oxygen. After carbonization, it is cooled to room temperature, crushed through an 80-mesh sieve, and a synergist is added and mixed to obtain a composite biochar.

[0019] (4) Granulation:

[0020] The microbial agent prepared in step (1) and the biochar prepared in step (3) are mixed evenly, and the mixture is added to the fermented organic matter prepared in step (2). Potassium fulvic acid, organic acid, fly ash and hawthorn extract are then added in sequence, and the mixture is stirred and mixed evenly. The mixture is dried to a moisture content of ≤15%, granulated by a granulator, and particles with a particle size of 3-5 mm are screened out to obtain a stress-resistant and growth-promoting bio-organic fertilizer.

[0021] The present invention also provides the use of the stress-resistant and growth-promoting bio-organic fertilizer in corn stress-resistant and growth-promoting in saline-alkali land.

[0022] The microbial agents include Bradyrhizobium Canariae, Pseudomonas Chengdui, and Bacillus megaterium. Bradyrhizobium Canariae improves soil nitrogen supply through nitrogen fixation and synthesizes various osmotic regulating substances, such as proline, to help plants cope with salt stress. Bacillus megaterium has strong salt tolerance and phosphorus solubilization properties, which can improve soil physical and chemical properties and promote plant growth. Pseudomonas Chengdui improves soil nutritional status by dissolving nutrients such as phosphorus and nitrogen in the soil, providing plants with sufficient nutrients and increasing their tolerance to various abiotic stresses. Furthermore, it produces plant hormones such as indoleacetic acid, which plays an important role in plant root development and overall growth. The three functional strains work synergistically to significantly improve soil quality, enhance soil fertility, strengthen plant resistance, and promote plant growth.

[0023] Organic acids are added to the organic fertilizer of the present invention. The organic acids can reduce the pH value of the soil, improve the acidity and alkalinity of the soil, and make it more suitable for crop growth. In addition, the organic acids can react with minerals in the soil to release required nutrients such as phosphorus and potassium, thereby improving soil fertility. The organic acids can also be directly absorbed and utilized by crops as a carbon source, thereby promoting the photosynthesis and material accumulation of the crops. At the same time, the organic acids can stimulate the growth of the crop root system, increase the root system's ability to absorb water and nutrients, and promote the growth and development of the crops.

[0024] Wheat straw biochar can significantly improve soil physical properties, such as increasing its water and fertilizer retention capacity and air permeability, while reducing its bulk density and density, thereby providing a more suitable growth environment for plants. Adding synergists to biochar, such as potassium nitrate, can improve plants' osmotic regulation, reducing salt damage to plants. It can also increase photosynthetic efficiency and stomatal conductance, thereby enhancing their absorption of water and mineral nutrients. Catechins can increase the activity of antioxidant enzymes in plants, thereby alleviating the damage caused by salt stress. Alginic acid can reduce the surface tension of water, making nutrients more easily absorbed by plants, promote root development, and improve photosynthetic efficiency. As a polysaccharide extracted from seaweed, it also provides energy for microorganisms, ensuring their high survival.

[0025] Hawthorn extract is rich in a variety of beneficial ingredients, mainly including organic acids, flavonoids, vitamins and trace elements, and also promotes plant growth and development. Hawthorn extract can regulate plant hormone levels, promote cell division and elongation, and enhance plant growth rate. The phenolic compounds in hawthorn extract have significant antioxidant, anti-inflammatory and stress resistance activities. These ingredients can help plants resist environmental stresses, such as saline and alkali adverse conditions. In addition, the flavonoids in hawthorn extract can enhance the antioxidant capacity of plants and reduce the damage to plant cells caused by oxidative stress, thereby improving the stress resistance of plants and helping plants maintain normal physiological activities in adverse environments.

[0026] Beneficial effects

[0027] The bio-organic fertilizer of the present invention comprises hawthorn extract, compound microbial agents, synergists and other ingredients, and multiple substances work synergistically to effectively reduce the salinity and alkalinity of the soil, significantly improve soil fertility, and at the same time enhance the stress resistance of crops, promote crop growth, increase crop yield, and improve planting benefits. DETAILED DESCRIPTION

[0028] The technical solution of the present invention will be further described below with reference to specific embodiments, but is not limited thereto.

[0029] Example 1

[0030] A stress-resistant and growth-promoting bio-organic fertilizer is prepared by including the following raw materials in parts by weight: 12 parts of microbial agent, 10 parts of potassium humate, 75 parts of wheat straw, 5 parts of organic acid, 45 parts of mushroom residue, 28 parts of dry sheep manure, 16 parts of corn cobs, 0.5 parts of urea, 3 parts of fly ash, 8 parts of synergist, and 5 parts of hawthorn extract.

[0031] The microbial agent is Bradyrhizobium of the Canary Islands, Bacillus megaterium and Pseudomonas chengduensis in a mass ratio of 1:1:1.

[0032] The strain number of the Canary Islands Bradyrhizobium is CGMCC No. 1.15558; the strain number of the Bacillus megaterium is CGMCC No. 1.10466; and the strain number of the Chengdu Pseudomonas is CGMCC No. 1.15626.

[0033] The organic acids are citric acid and malic acid in a mass ratio of 1:1.

[0034] The organic acid is diluted 5 times before use.

[0035] The synergist comprises 5 parts of potassium nitrate, 2 parts of catechin and 3 parts of alginic acid.

[0036] A method for preparing stress-resistant and growth-promoting bio-organic fertilizer comprises the following steps:

[0037] (1) Preparation of microbial agents:

[0038] After activation, Bradyrhizobium Canariae, Pseudomonas Chengdui, and Bacillus megaterium were inoculated into LB liquid medium at 28°C, 30°C, and 30°C respectively until OD 600 =0.6 to obtain seed solution, 1% of the Canary Islands Bradyrhizobium seed solution was inoculated into nitrogen-fixing medium, and 1% of Chengdu Pseudomonas and Bacillus megaterium were inoculated into LB liquid medium respectively, and cultured in the medium until the number of viable bacteria reached 1×10 9 cfu·mL -1 obtaining three bacterial solutions, then uniformly mixing the three bacterial solutions in a mass ratio of 1:1:1, and freeze-drying them into freeze-dried powder to obtain a microbial agent;

[0039] (2) Fermentation and composting:

[0040] The mushroom residue, corn cobs, and dry sheep manure are crushed and passed through a 50-mesh sieve, mixed evenly, placed in a fermentation tank, and water is added and mixed evenly to control the moisture content between 50% and 60%. Urea is then placed in the fermentation tank and stirred evenly. The fermentation tank is sealed with a plastic film. The fermentation process is completed once every 7 days. After 3 turnings, the fermentation is completed to obtain fermented organic matter.

[0041] (3) Preparation of compound biochar:

[0042] The wheat straw is dried and crushed to 2 cm, added to a reactor, and carbonized at 300-500°C for 2 hours without oxygen. After carbonization, it is cooled to room temperature, crushed through an 80-mesh sieve, and a synergist is added and mixed to obtain a composite biochar.

[0043] (4) Granulation:

[0044] The microbial agent prepared in step (1) and the biochar prepared in step (3) are mixed evenly, and the mixture is added to the fermented organic matter prepared in step (2). Potassium fulvic acid, organic acid, fly ash and hawthorn extract are then added in sequence, and the mixture is stirred and mixed evenly. The mixture is dried to a moisture content of ≤15%, granulated by a granulator, and particles with a particle size of 3-5 mm are screened out to obtain a stress-resistant and growth-promoting bio-organic fertilizer.

[0045] Example 2

[0046] A stress-resistant and growth-promoting bio-organic fertilizer is prepared by including the following raw materials in parts by weight: 15 parts of microbial agent, 12 parts of potassium fulvic acid, 82 parts of wheat straw, 7 parts of organic acid, 50 parts of mushroom residue, 32 parts of dry sheep manure, 18 parts of corn cobs, 0.8 parts of urea, 4 parts of fly ash, 9 parts of synergist, and 6 parts of hawthorn extract.

[0047] The microbial agent is Bradyrhizobium of the Canary Islands, Bacillus megaterium and Pseudomonas chengduensis in a mass ratio of 1:1:1.

[0048] The strain number of the Canary Islands Bradyrhizobium is CGMCC No. 1.15558; the strain number of the Bacillus megaterium is CGMCC No. 1.10466; and the strain number of the Chengdu Pseudomonas is CGMCC No. 1.15626.

[0049] The organic acids are citric acid and malic acid in a mass ratio of 1:1.

[0050] The organic acid is diluted 5 times before use.

[0051] The synergist comprises 5 parts of potassium nitrate, 2 parts of catechin and 3 parts of alginic acid.

[0052] A method for preparing stress-resistant and growth-promoting bio-organic fertilizer comprises the following steps:

[0053] (1) Preparation of microbial agents:

[0054] After activation, Bradyrhizobium Canariae, Pseudomonas Chengdui, and Bacillus megaterium were inoculated into LB liquid medium at 28°C, 30°C, and 30°C respectively until OD 600 =0.6 to obtain seed solution, 1% of the Canary Islands Bradyrhizobium seed solution was inoculated into nitrogen-fixing medium, and 1% of Chengdu Pseudomonas and Bacillus megaterium were inoculated into LB liquid medium respectively, and cultured in the medium until the number of viable bacteria reached 1×10 9 cfu·mL -1 obtaining three bacterial solutions, then uniformly mixing the three bacterial solutions in a mass ratio of 1:1:1, and freeze-drying them into freeze-dried powder to obtain a microbial agent;

[0055] (2) Fermentation and composting:

[0056] The mushroom residue, corn cobs, and dry sheep manure are crushed and passed through a 50-mesh sieve, mixed evenly, placed in a fermentation tank, and water is added and mixed evenly to control the moisture content between 50% and 60%. Urea is then placed in the fermentation tank and stirred evenly. The fermentation tank is sealed with a plastic film. The fermentation process is completed once every 7 days. After 3 turnings, the fermentation is completed to obtain fermented organic matter.

[0057] (3) Preparation of compound biochar:

[0058] The wheat straw is dried and crushed to 2 cm, added to a reactor, and carbonized at 300-500°C for 2 hours without oxygen. After carbonization, it is cooled to room temperature, crushed through an 80-mesh sieve, and a synergist is added and mixed to obtain a composite biochar.

[0059] (4) Granulation:

[0060] The microbial agent prepared in step (1) and the biochar prepared in step (3) are mixed evenly, and the mixture is added to the fermented organic matter prepared in step (2). Potassium fulvic acid, organic acid, fly ash and hawthorn extract are then added in sequence, and the mixture is stirred and mixed evenly. The mixture is dried to a moisture content of ≤15%, granulated by a granulator, and particles with a particle size of 3-5 mm are screened out to obtain a stress-resistant and growth-promoting bio-organic fertilizer.

[0061] Example 3

[0062] A stress-resistant and growth-promoting bio-organic fertilizer is prepared by including the following raw materials in parts by weight: 18 parts of microbial agent, 15 parts of potassium humate, 90 parts of wheat straw, 10 parts of organic acid, 55 parts of mushroom residue, 36 parts of dry sheep manure, 22 parts of corn cobs, 1 part of urea, 5 parts of fly ash, 10 parts of synergist, and 8 parts of hawthorn extract.

[0063] The microbial agent is Bradyrhizobium of the Canary Islands, Bacillus megaterium and Pseudomonas chengduensis in a mass ratio of 1:1:1.

[0064] The strain number of the Canary Islands Bradyrhizobium is CGMCC No. 1.15558; the strain number of the Bacillus megaterium is CGMCC No. 1.10466; and the strain number of the Chengdu Pseudomonas is CGMCC No. 1.15626.

[0065] The organic acids are citric acid and malic acid in a mass ratio of 1:1.

[0066] The organic acid is diluted 5 times before use.

[0067] The synergist comprises 5 parts of potassium nitrate, 2 parts of catechin and 3 parts of alginic acid.

[0068] A method for preparing stress-resistant and growth-promoting bio-organic fertilizer comprises the following steps:

[0069] (1) Preparation of microbial agents:

[0070] After activation, Bradyrhizobium Canariae, Pseudomonas Chengdui, and Bacillus megaterium were inoculated into LB liquid medium at 28°C, 30°C, and 30°C respectively until OD 600 =0.6 to obtain seed solution, 1% of the Canary Islands Bradyrhizobium seed solution was inoculated into nitrogen-fixing medium, and 1% of Chengdu Pseudomonas and Bacillus megaterium were inoculated into LB liquid medium respectively, and cultured in the medium until the number of viable bacteria reached 1×10 9 cfu·mL -1 obtaining three bacterial solutions, then uniformly mixing the three bacterial solutions in a mass ratio of 1:1:1, and freeze-drying them into freeze-dried powder to obtain a microbial agent;

[0071] (2) Fermentation and composting:

[0072] The mushroom residue, corn cobs, and dry sheep manure are crushed and passed through a 50-mesh sieve, mixed evenly, placed in a fermentation tank, and water is added and mixed evenly to control the moisture content between 50% and 60%. Urea is then placed in the fermentation tank and stirred evenly. The fermentation tank is sealed with a plastic film. The fermentation process is completed once every 7 days. After 3 turnings, the fermentation is completed to obtain fermented organic matter.

[0073] (3) Preparation of compound biochar:

[0074] The wheat straw is dried and crushed to 2 cm, added to a reactor, and carbonized at 300-500°C for 2 hours without oxygen. After carbonization, it is cooled to room temperature, crushed through an 80-mesh sieve, and a synergist is added and mixed to obtain a composite biochar.

[0075] (4) Granulation:

[0076] The microbial agent prepared in step (1) and the biochar prepared in step (3) are mixed evenly, and the mixture is added to the fermented organic matter prepared in step (2). Potassium fulvic acid, organic acid, fly ash and hawthorn extract are then added in sequence, and the mixture is stirred and mixed evenly. The mixture is dried to a moisture content of ≤15%, granulated by a granulator, and particles with a particle size of 3-5 mm are screened out to obtain a stress-resistant and growth-promoting bio-organic fertilizer.

[0077] Comparative Example 1

[0078] Compared with Example 3, this comparative example has the same raw materials and steps as Example 3 except that no synergist is used.

[0079] Comparative Example 2

[0080] Compared with Example 3, this comparative example is the same as Example 3 except that catechin is not used as the synergist. Other raw materials and steps are the same as Example 3.

[0081] Comparative Example 3

[0082] Compared with Example 3, this comparative example is the same as Example 3 except that alginic acid is not used as the synergist. The other raw materials and steps are the same as those of Example 3.

[0083] Comparative Example 4

[0084] Compared with Example 3, this comparative example is the same as Example 3 except that potassium nitrate is not used as the synergist. The other raw materials and steps are the same as those of Example 3.

[0085] Comparative Example 5

[0086] Compared with Example 3, this comparative example is the same as Example 3 except that Bradyrhizobium Canariae is not used in the preparation of the microbial agent. Other raw materials and steps are the same as those in Example 3.

[0087] Comparative Example 6

[0088] Compared with Example 3, this comparative example uses the same raw materials and steps as Example 3, except that Pseudomonas chengduensis is not used in the preparation of the microbial agent.

[0089] Comparative Example 7

[0090] Compared with Example 3, this comparative example is the same as Example 3 except that Bacillus megaterium is not used in the preparation of the microbial agent. The other raw materials and steps are the same as those in Example 3.

[0091] Comparative Example 8

[0092] Compared with Example 3, this comparative example is the same as Example 3 except that the hawthorn extract is not used. The other raw materials and steps are the same as Example 3.

[0093] Performance Testing

[0094] Field trials:

[0095] A field experiment was conducted on saline-alkali land in Xiaobotou Town, Wudi County, Binzhou City, Shandong Province. The soil was brown soil with an average pH of 8.5, a soil organic matter content of 7.2 g / kg, and a soil bulk density of 1.54 g / cm 3 , field water holding capacity 16.7%, water-soluble salt 3.1g / kg.

[0096] The experiment set up 12 treatment groups, each covering 2 mu. The bio-organic fertilizers prepared in Examples 1-3 of the present invention and Comparative Examples 1-8 were applied to each test plot, and one test plot was used as a control group to apply conventional compound fertilizer (nitrogen, phosphorus, and potassium in a ratio of 32:10:5). Before sowing corn, the organic fertilizer and compound fertilizer were evenly spread on the surface and then rotated into the soil layer using a rotary tiller. The rotary tillage depth was about 15 cm. The amount of organic fertilizer and conventional fertilizer was 100 kg per mu. The corn variety was Golden Grain MY73, and the corn sowing density was 4,000 plants / mu. A unified field management method was adopted during corn planting. After the corn matured, 5 sampling points were selected for each treatment group, and 10 ears were continuously sampled from each sampling point. The plant height and stem diameter of each group of corn were counted. The ears were harvested from an area of ​​10 m × 10 m in each treatment group. The ears were dried and threshed. After threshing, the kernels were dried and the thousand-kernel weight was measured to calculate the yield. The statistical data are shown in Table 1.

[0097] Table 1 Agronomic traits and yield of corn

[0098]

[0099] According to the data in Table 1, compared with the control group where conventional fertilizers were applied, the plant height and stem diameter of the corn plants were significantly improved, and the 1000-grain weight and yield of the corn were also significantly improved when the bio-organic fertilizers prepared in Examples 1-3 of the present invention were applied. However, the plant traits and yield of the comparative examples 1-8 where the components of the bio-organic fertilizers were changed decreased to varying degrees, indicating that the components of the bio-organic fertilizers prepared in the present invention work synergistically to effectively promote the growth of corn plants and increase yield.

[0100] After corn harvest, soil physical and chemical properties were tested for the 12 treatment groups. Soil was collected from the 0-20 cm soil layer near the corn roots in each experimental group. Five points were randomly selected for collection in each experimental group. 1 kg of mixed samples were taken for measurement of pH value, organic matter, bulk density, total water-soluble salts, and field water holding capacity. The statistical data are shown in Table 2.

[0101] The specific detection methods are as follows:

[0102] pH value: using the acidometer method (water: soil = 5:1);

[0103] Bulk density and field capacity: Bulk density is measured using the ring knife method and field capacity is calculated;

[0104] Organic matter: using potassium dichromate method;

[0105] Total amount of water-soluble salt: Use a conductivity meter to measure the conductivity of the extracted solution and convert it into the total salt content of the soil.

[0106] Table 2 Physical and chemical properties of soil

[0107]

[0108] As shown in Table 2 data, the bio-organic fertilizer prepared by applying the embodiment of the present invention 1-3 can significantly reduce the pH value of soil, reduce soil bulk density, reduce the total amount of water-soluble salts, improve soil organic matter and field capacity, and significantly improve soil physicochemical properties. Compared with original soil, applying the bio-organic fertilizer of the embodiment of the present invention 1-3, the pH of soil has reduced by about 1, and the total amount of water-soluble salts also significantly decreases, illustrating that the salinity of soil can be improved using bio-organic fertilizer of the present invention, and the improvement of field capacity and bulk density has improved the porosity of soil in addition, effectively improving the physicochemical properties of saline-alkali soil. Compared with the bio-organic fertilizer of application example 3, after applying the bio-organic fertilizer of the comparative example 1-8 that changes constituent, each index all has various degrees of weakening, and therefore, each component of microbial inoculant, hawthorn extract and synergist all plays an indispensable role in the improvement of saline-alkali land, and lacking one is weak in effect.

[0109] It should be noted that the above embodiments are only some of the preferred embodiments of the present invention, and not all of them. Obviously, based on the above embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work should fall within the scope of protection of the present invention.

Claims

1. A stress-resistant and growth-promoting bio-organic fertilizer, characterized in that: The invention comprises the following raw materials in parts by weight: 12-18 parts of microbial agent, 10-15 parts of potassium fulvic acid, 75-90 parts of wheat straw, 5-10 parts of organic acid, 45-55 parts of mushroom residue, 28-36 parts of dry sheep manure, 16-22 parts of corn cobs, 0.5-1 part of urea, 3-5 parts of fly ash, 8-10 parts of synergist, and 5-8 parts of hawthorn extract; The microbial agent is Bradyrhizobium canariense, Bacillus megaterium, and Pseudomonas chengduensis in a mass ratio of 1:1:1; the strain number of Bradyrhizobium canariense is CGMCC No. 1.15558; the strain number of Bacillus megaterium is CGMCC No. 1.10466; and the strain number of Pseudomonas chengduensis is CGMCC No. 1.15626. The synergist comprises 5 parts of potassium nitrate, 2 parts of catechins, and 3 parts of alginic acid; The stress-resistant and growth-promoting biological organic fertilizer is used for improving the stress-resistant and growth-promoting properties of corn in saline-alkali land.

2. The stress-resistant and growth-promoting bio-organic fertilizer according to claim 1, wherein The organic acids are citric acid and malic acid in a mass ratio of 1:

1.

3. The stress-resistant and growth-promoting bio-organic fertilizer according to claim 1, wherein The organic acid is diluted 5 times before use.

4. A method for preparing the stress-resistant and growth-promoting bio-organic fertilizer according to any one of claims 1 to 3, characterized in that: The following steps are involved: (1) Preparation of microbial agents: After activation, Bradyrhizobium canariensis, Pseudomonas chengduensis, and Bacillus megaterium were respectively inoculated into LB liquid culture medium and cultured at 28°C, 30°C, and 30°C until OD600 = 0.6 to obtain seed liquid, 1% of the Bradyrhizobium canariensis seed liquid was inoculated into nitrogen-fixing medium, and 1% of Pseudomonas chengduensis and Bacillus megaterium were respectively inoculated into LB liquid culture medium, and the cultures were cultured in the culture medium until the number of viable bacteria reached 1×109 cfu·mL-1 to obtain three bacterial liquids, and then the three bacterial liquids were evenly mixed in a mass ratio of 1:1:1, and freeze-dried into freeze-dried powder to obtain a microbial agent; (2) Fermentation and composting: The mushroom residue, corn cobs, and dry sheep manure are crushed and passed through a 50-mesh sieve, mixed evenly, placed in a fermentation tank, and water is added and mixed evenly to control the moisture content between 50% and 60%. Urea is then placed in the fermentation tank and stirred evenly. The fermentation tank is sealed with a plastic film. The fermentation process is completed once every 7 days. After 3 turnings, the fermentation is completed to obtain fermented organic matter. (3) Preparation of compound biochar: The wheat straw is dried and crushed to 2 cm, added to a reactor, and carbonized at 300-500°C for 2 hours without oxygen. After carbonization, it is cooled to room temperature, crushed through an 80-mesh sieve, and a synergist is added and mixed to obtain a composite biochar. (4) Granulation: The microbial agent prepared in step (1) and the biochar prepared in step (3) are mixed evenly, and the mixture is added to the fermented organic matter prepared in step (2). Potassium fulvic acid, organic acid, fly ash and hawthorn extract are then added in sequence, and the mixture is stirred and mixed evenly. The mixture is dried to a moisture content of ≤15%, granulated by a granulator, and particles with a particle size of 3-5 mm are screened out to obtain a stress-resistant and growth-promoting bio-organic fertilizer.

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