Tobacco product lung injury evaluation method based on murine lung organs

By establishing an evaluation method based on murine lung organoids, the limitations of lung injury assessment of tobacco products in the prior art were solved, and rapid and accurate lung injury assessment was achieved, revealing the differences in the damage effect of different tobacco products.

CN120400295APending Publication Date: 2025-08-01HUBEI CHINA TOBACCO INDUSTRY CO LTD
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Patent Information

Application Number
CN202510563454.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-04-30
Publication Date
2025-08-01

AI Technical Summary

Technical Problem

Existing cellular and animal models have limitations in the assessment of lung injury in tobacco products, making it difficult to achieve rapid and effective high-throughput screening experiments, and traditional poisoning methods are prone to false negative results.

Method used

Establish a lung injury evaluation method for tobacco products based on mouse-derived lung organoids, including preparing tobacco products traps, inducing mouse lung tissue culture using MAOs culture medium, performing pre-virus, setting concentration gradient infection and evaluating changes in lung organoid physiological indicators.

Benefits of technology

It provides a fast and effective method for evaluating lung injury of tobacco products, which can simulate the impact of tobacco products on lung damage, evaluate the damage effect through changes in reactive oxygen species and tissue structure, reflect the effect differences between different tobacco products, and provide accurate assessment for toxicological research.

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Abstract

The invention discloses a tobacco product lung injury evaluation method based on murine lung organs, which comprises the following steps: step A, preparing a trapping substance by using a tobacco product to obtain the tobacco product trapping substance; step B, performing induction culture on the mouse lung tissue by using an MAOs culture medium to obtain mouse lung organs; step C, pre-poisoning the lung organs of the mouse by using the tobacco product traps to determine IC50 and poisoning time of the tobacco product traps; and D, setting a concentration gradient according to the IC50 of the tobacco product traps, contaminating the lung organs of the mouse, and evaluating the lung injury caused by the tobacco product traps according to the physiological index change of the lung organs of the mouse. According to the method for evaluating the lung injury of the tobacco product by using the mouse lung organ, the influence of exposure of the tobacco product on the lung injury can be simulated, the effect difference between different tobacco products is reflected, and a more accurate evaluation method is provided for toxicological research of the tobacco product.
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