Biochemically modified bacteriostatic nonwoven fabric and method for producing the same

Abstract

This invention relates to the field of nonwoven fabric preparation technology, specifically to a biochemically modified antibacterial nonwoven fabric and its preparation method. This invention modifies nano-zinc oxide and nano-titanium dioxide using the silane coupling agent KH-570, improving the dispersibility of the nanoparticles. Under light or contact conditions, the nanoparticles release reactive oxygen species to disrupt bacterial cell membranes and inhibit their metabolism, thereby giving the antibacterial modified polypropylene masterbatch stable antibacterial properties. Furthermore, this invention stabilizes the loading through a polydopamine coating, thus stabilizing the antibacterial properties of lysozyme. Lysozyme specifically hydrolyzes the peptidoglycan layer of bacterial cell walls, leading to bacterial lysis. Thiourea dioxide, as a reducing agent, maintains lysozyme activity, preventing oxidative inactivation, while simultaneously inhibiting bacterial metabolic enzyme activity. Through the synergistic effect of inorganic and biological dual-effects, this invention can cover Gram-positive bacteria, Gram-negative bacteria, and fungi, thereby improving the antibacterial properties of the nonwoven fabric to a certain extent.

Description

Technical Field

[0001] This invention relates to the field of nonwoven fabric preparation technology, specifically to a biochemically modified antibacterial nonwoven fabric and its preparation method. Background Technology

[0002] Nonwoven fabrics, as a high-efficiency and low-cost nonwoven material, are widely used in medical protection, hygiene products, and filtration. However, the antibacterial properties of traditional nonwoven fabrics mainly rely on physical barriers or finishing coatings, which present the following technical bottlenecks: First, the antibacterial activity is singular and easily leads to drug resistance. For example, silver-based antibacterial agents kill bacteria only by releasing metal ions, and long-term use may lead to increased drug resistance in microorganisms. Organic antibacterial agents, such as quaternary ammonium salts, are easily decomposed by environmental pH and temperature, resulting in insufficient antibacterial durability. Second, chemical modification processes are complex and have high ecological risks. For example, when introducing antibacterial components through blending or surface grafting in existing technologies, high temperature and pressure or toxic solvents are often required, leading to nanoparticle aggregation, deterioration of fiber mechanical properties, and residual solvents that may cause biotoxicity. Third, the loading efficiency of bioactive components is low. If nonwoven fabrics are directly treated with biomolecules such as lysozyme, the enzyme loading is low and easily detached due to the inertness of the fiber surface, making it difficult to achieve long-term antibacterial effect.

[0003] To address the aforementioned issues, those skilled in the art have recently attempted to combine inorganic antibacterial agents with bioactive substances. However, this technological integration still suffers from many shortcomings. For example, the synergistic mechanism between nano-zinc oxide / titanium dioxide and lysozyme is unclear, and both exhibit poor dispersion stability in fiber matrices. While polydopamine can improve material surface adhesion, its film-forming uniformity and biomolecule immobilization efficiency on hydrophobic substrates still require optimization. Therefore, how to modify these technological deficiencies has become a pressing technical problem for those skilled in the art. Summary of the Invention

[0004] Technical problems to be solved

[0005] In view of the above-mentioned shortcomings of the prior art, the present invention provides a biochemically modified antibacterial nonwoven fabric and its preparation method, which can effectively solve the problem that the antibacterial performance of the prior art is not durable and stable.

[0006] Technical solution

[0007] To achieve the above objectives, the present invention provides the following technical solution:

[0008] A method for preparing a biochemically modified antibacterial nonwoven fabric, wherein the method for preparing the biochemically modified antibacterial nonwoven fabric is as follows:

[0009] S1. Polypropylene nonwoven fabric is made by melt-blowing antibacterial modified polypropylene masterbatch as raw material.

[0010] S2. Polypropylene nonwoven fabric is treated with alkali and then treated with polydopamine. The result is called pretreated nonwoven fabric.

[0011] S3. Immerse the pretreated nonwoven fabric in lysozyme solution, soak it at 37°C for 1 hour, rinse it with pure water and dry it. This is counted as one finishing process. After four finishing processes, the resulting biochemically modified antibacterial nonwoven fabric is obtained.

[0012] Furthermore, the preparation steps of the antibacterial modified polypropylene masterbatch in S1 are as follows:

[0013] Step 1: Dissolve 2 parts by weight of silane coupling agent KH-570 in 500 parts by weight of ethanol aqueous solution, adjust the pH value to 4-5 with glacial acetic acid, stir and disperse, add 10 parts by weight of nano zinc oxide and 10 parts by weight of nano titanium dioxide, sonicate and then stir magnetically, filter and wash 3 times with anhydrous ethanol, dry and grind through a 200-mesh sieve. The resulting product is recorded as the inorganic antibacterial component.

[0014] Step 2: Pour 80-90 parts by weight of polypropylene and 20-25 parts by weight of inorganic antibacterial component into a high-speed mixer and mix evenly. After granulation by twin-screw extrusion, the resulting product is the antibacterial modified polypropylene masterbatch.

[0015] Furthermore, in step 1, the volume fraction of the ethanol-water solution is 90%, the stirring and dispersion method is to stir at a speed of 300-500 r / min for 30 min, the ultrasonic treatment method is to ultrasonically disperse at a power of 200-300 W for 30 min, the magnetic stirring method is to stir at a speed of 500-600 r / min for 2-4 h, and the drying treatment method is to dry at a temperature of 70-80℃ until constant weight.

[0016] Furthermore, in step 2, the processing temperature of the twin-screw extrusion is 170-190℃, the main screw speed is 300r / min, and the feed screw speed is 20r / min.

[0017] Furthermore, the preparation method of the polypropylene nonwoven fabric in S1 is as follows:

[0018] Polypropylene and antibacterial modified polypropylene masterbatch were melt-blended at a weight ratio of 19:1, and then melt-blown to produce polypropylene nonwoven fabric. The temperature of the melt-blown spinning machine was 240-260℃, the receiving distance of the meltblown fabric was 300mm, the hot air pressure was 0.6MPa, and the winding speed was 5m / min.

[0019] Furthermore, the method for alkali treatment in S2 is as follows:

[0020] The polypropylene nonwoven fabric is immersed in a 5% sodium hydroxide solution at 80℃ for 2 hours, drained, and rinsed 3 times with pure water. Then it is immersed in a 1 mL / L acetic acid solution at room temperature for 10 minutes, rinsed 3-5 times with pure water, and dried at 55-60℃ for 2-3 hours to complete the alkali treatment.

[0021] Furthermore, the method for treating polydopamine in S2 is as follows:

[0022] Weigh 20g of dopamine hydrochloride and dissolve it in 10L of 0.01mol / L Tris buffer. Adjust the pH to 8.5 with 0.1mol / L hydrochloric acid and then immerse it in alkali-treated polypropylene nonwoven fabric. Shake in a 30℃ water bath for 24 hours, rinse with pure water 3-5 times, and dry at 30-35℃ for 5-8 hours to complete the polydopamine treatment.

[0023] Furthermore, the method for preparing the lysozyme solution in S3 is as follows:

[0024] Weigh 10g of thiourea dioxide and dissolve it in 5L of 0.01mol / L Tris buffer. Adjust the pH to 7.4 with 0.1mol / L hydrochloric acid, add 10g of lysozyme, and stir at 100r / min for 5min. The resulting solution is the lysozyme solution.

[0025] Furthermore, in S3, the rinsing and drying process involves 2-3 rinses with pure water, and the drying temperature and time are 60℃ and 1h, respectively.

[0026] A biochemically modified antibacterial nonwoven fabric is prepared by a method for preparing a biochemically modified antibacterial nonwoven fabric.

[0027] Beneficial effects

[0028] This invention provides a biochemically modified antibacterial nonwoven fabric and its preparation method. Compared with existing technologies, this invention has the following advantages:

[0029] 1. This invention modifies nano-zinc oxide and nano-titanium dioxide using the silane coupling agent KH-570, thereby improving the dispersibility of the nanoparticles. Under light or contact conditions, the nanoparticles release reactive oxygen species to destroy bacterial cell membranes and inhibit their metabolism, thus giving the antibacterial modified polypropylene masterbatch stable antibacterial properties. This invention also stabilizes the loading through a polydopamine coating, thereby stabilizing the antibacterial properties of lysozyme. Lysozyme can specifically hydrolyze the peptidoglycan layer of bacterial cell walls, leading to bacterial lysis. Thiourea dioxide, as a reducing agent, can maintain the activity of lysozyme, preventing oxidative inactivation, while inhibiting the activity of bacterial metabolic enzymes. Through the synergistic effect of inorganic and biological dual effects, this invention can cover Gram-positive bacteria, Gram-negative bacteria, and fungi, thereby improving the antibacterial properties of nonwoven fabrics to a certain extent.

[0030] 2. This invention increases the surface roughness of polypropylene through alkali treatment, allowing polydopamine to adhere firmly through stacking and hydrogen bonding, forming a stable adhesive layer that prevents lysozyme from detaching. The multiple loading processes of four impregnation treatments create a gradient coating, embedding lysozyme molecules within the three-dimensional network structure of polydopamine, thereby enhancing sustained-release performance and maintaining the durability of antibacterial properties. The material in this invention possesses antibacterial, antifouling, and self-cleaning properties, making it applicable to a wider range of fields and achieving greater application value. Detailed Implementation

[0031] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0032] The present invention will be further described below with reference to embodiments.

[0033] The sources of some components in the examples and comparative examples are as follows:

[0034] Polypropylene, model Z30S, Sinopec Xinjiang Dushanzi Petrochemical Co., Ltd.;

[0035] Silane coupling agent KH-570, Shandong Rongsheng New Materials Co., Ltd.;

[0036] Nano zinc oxide, model MS-K003, Guangzhou Zanyu Anti-mildew Technology Co., Ltd.

[0037] Nano titanium dioxide, Jinan Hongquan Titanium Industry Co., Ltd.;

[0038] Dopamine hydrochloride, Aladdin Reagent Co., Ltd.;

[0039] Tris buffer, Sinopharm Chemical Reagent Co., Ltd.

[0040] Thiourea dioxide, Aladdin Reagent Co., Ltd.;

[0041] Lysozyme, 20000U / mg, Guangxi Pangbo Biotechnology Co., Ltd.

[0042] Example 1

[0043] This embodiment describes a method for preparing a biochemically modified antibacterial nonwoven fabric. The method for preparing the biochemically modified antibacterial nonwoven fabric is as follows:

[0044] S1. Polypropylene nonwoven fabric is made by melt-blowing antibacterial modified polypropylene masterbatch as raw material.

[0045] The preparation steps of the antibacterial modified polypropylene masterbatch are as follows:

[0046] Step 1: Dissolve 2 parts by weight of silane coupling agent KH-570 in 500 parts by weight of 90% ethanol aqueous solution, adjust the pH value to 4 with glacial acetic acid, stir at 300 r / min for 30 min, add 10 parts by weight of nano zinc oxide and 10 parts by weight of nano titanium dioxide, ultrasonically disperse at 200 W for 30 min, stir at 500 r / min for 2 h, filter, wash 3 times with anhydrous ethanol, dry at 70℃ to constant weight, grind and pass through a 200 mesh sieve. The resulting product is recorded as the inorganic antibacterial component.

[0047] Step 2: Pour 80 parts by weight of polypropylene and 20 parts by weight of inorganic antibacterial component into a high-speed mixer and mix evenly. After granulation by twin-screw extrusion, the resulting product is antibacterial modified polypropylene masterbatch. The processing temperature of twin-screw extrusion is 170℃, the main screw speed is 300r / min, and the feed screw speed is 20r / min.

[0048] The preparation method of polypropylene nonwoven fabric is as follows:

[0049] Polypropylene and antibacterial modified polypropylene masterbatch were melt-blended at a weight ratio of 19:1, and then melt-blown to produce polypropylene nonwoven fabric. The temperature of the melt-blown spinning machine was 240℃, the receiving distance of the meltblown fabric was 300mm, the hot air pressure was 0.6MPa, and the winding speed was 5m / min.

[0050] S2. Polypropylene nonwoven fabric is treated with alkali and then treated with polydopamine. The result is called pretreated nonwoven fabric.

[0051] The alkaline treatment method is as follows:

[0052] The polypropylene nonwoven fabric was immersed in a 5% sodium hydroxide solution at 80°C for 2 hours, drained, and rinsed three times with pure water. Then it was immersed in a 1 mL / L acetic acid solution at room temperature for 10 minutes, rinsed three times with pure water again, and dried at 55°C for 2 hours to complete the alkali treatment.

[0053] The method for polydopamine treatment is as follows:

[0054] Weigh 20g of dopamine hydrochloride and dissolve it in 10L of 0.01mol / L Tris buffer. Adjust the pH to 8.5 with 0.1mol / L hydrochloric acid and then immerse it in alkali-treated polypropylene nonwoven fabric. Shake in a 30℃ water bath for 24 hours, rinse three times with pure water, and dry at 30℃ for 5 hours to complete the polydopamine treatment.

[0055] S3. Immerse the pretreated nonwoven fabric in lysozyme solution, soak it at 37°C for 1 hour, rinse it with pure water and dry it. This is counted as one finishing process. After four finishing processes, the resulting biochemically modified antibacterial nonwoven fabric is obtained. The number of rinsing and drying with pure water is 2, and the drying temperature and time are 60°C and 1 hour, respectively.

[0056] The preparation method of the lysozyme solution is as follows:

[0057] Weigh 10g of thiourea dioxide and dissolve it in 5L of 0.01mol / L Tris buffer. Adjust the pH to 7.4 with 0.1mol / L hydrochloric acid, add 10g of lysozyme, and stir at 100r / min for 5min. The resulting solution is the lysozyme solution.

[0058] A biochemically modified antibacterial nonwoven fabric is prepared by a method for preparing a biochemically modified antibacterial nonwoven fabric.

[0059] Example 2

[0060] This embodiment describes a method for preparing a biochemically modified antibacterial nonwoven fabric. The method for preparing the biochemically modified antibacterial nonwoven fabric is as follows:

[0061] S1. Polypropylene nonwoven fabric is made by melt-blowing antibacterial modified polypropylene masterbatch as raw material.

[0062] The preparation steps of the antibacterial modified polypropylene masterbatch are as follows:

[0063] Step 1: Dissolve 2 parts by weight of silane coupling agent KH-570 in 500 parts by weight of 90% ethanol aqueous solution, adjust the pH value to 5 with glacial acetic acid, stir at 500 r / min for 30 min, add 10 parts by weight of nano zinc oxide and 10 parts by weight of nano titanium dioxide, ultrasonically disperse at 300W for 30 min, stir at 600 r / min for 4 h, filter, wash 3 times with anhydrous ethanol, dry at 80℃ to constant weight, grind and pass through a 200 mesh sieve. The resulting product is recorded as the inorganic antibacterial component.

[0064] Step 2: Pour 90 parts by weight of polypropylene and 25 parts by weight of inorganic antibacterial component into a high-speed mixer and mix evenly. After granulation by twin-screw extrusion, the resulting product is antibacterial modified polypropylene masterbatch. The processing temperature of twin-screw extrusion is 190℃, the main screw speed is 300r / min, and the feed screw speed is 20r / min.

[0065] The preparation method of polypropylene nonwoven fabric is as follows:

[0066] Polypropylene and antibacterial modified polypropylene masterbatch were melt-blended at a weight ratio of 19:1, and then melt-blown to produce polypropylene nonwoven fabric. The temperature of the melt-blown spinning machine was 260℃, the receiving distance of the meltblown fabric was 300mm, the hot air pressure was 0.6MPa, and the winding speed was 5m / min.

[0067] S2. Polypropylene nonwoven fabric is treated with alkali and then treated with polydopamine. The result is called pretreated nonwoven fabric.

[0068] The alkaline treatment method is as follows:

[0069] The polypropylene nonwoven fabric was immersed in a 5% sodium hydroxide solution at 80°C for 2 hours, drained, and rinsed three times with pure water. Then it was immersed in a 1 mL / L acetic acid solution at room temperature for 10 minutes, rinsed five times with pure water, and dried at 60°C for 3 hours to complete the alkali treatment.

[0070] The method for polydopamine treatment is as follows:

[0071] Weigh 20g of dopamine hydrochloride and dissolve it in 10L of 0.01mol / L Tris buffer. Adjust the pH to 8.5 with 0.1mol / L hydrochloric acid and then immerse it in alkali-treated polypropylene nonwoven fabric. Shake in a 30℃ water bath for 24 hours, rinse 5 times with pure water, and dry at 35℃ for 8 hours to complete the polydopamine treatment.

[0072] S3. Immerse the pretreated nonwoven fabric in lysozyme solution, soak it at 37°C for 1 hour, rinse it with pure water and dry it. This is counted as one finishing process. After four finishing processes, the resulting biochemically modified antibacterial nonwoven fabric is obtained. The number of times to rinse with pure water and dry is three, and the drying temperature and time are 60°C and 1 hour, respectively.

[0073] The preparation method of the lysozyme solution is as follows:

[0074] Weigh 10g of thiourea dioxide and dissolve it in 5L of 0.01mol / L Tris buffer. Adjust the pH to 7.4 with 0.1mol / L hydrochloric acid, add 10g of lysozyme, and stir at 100r / min for 5min. The resulting solution is the lysozyme solution.

[0075] A biochemically modified antibacterial nonwoven fabric is prepared by a method for preparing a biochemically modified antibacterial nonwoven fabric.

[0076] Example 3

[0077] This embodiment describes a method for preparing a biochemically modified antibacterial nonwoven fabric. The method for preparing the biochemically modified antibacterial nonwoven fabric is as follows:

[0078] S1. Polypropylene nonwoven fabric is made by melt-blowing antibacterial modified polypropylene masterbatch as raw material.

[0079] The preparation steps of the antibacterial modified polypropylene masterbatch are as follows:

[0080] Step 1: Dissolve 2 parts by weight of silane coupling agent KH-570 in 500 parts by weight of 90% ethanol aqueous solution, adjust the pH value to 5 with glacial acetic acid, stir at 400 r / min for 30 min, add 10 parts by weight of nano zinc oxide and 10 parts by weight of nano titanium dioxide, ultrasonically disperse at 300 W for 30 min, stir at 600 r / min for 3 h, filter, wash 3 times with anhydrous ethanol, dry at 75℃ to constant weight, grind and pass through a 200 mesh sieve. The resulting product is recorded as the inorganic antibacterial component.

[0081] Step 2: Pour 85 parts by weight of polypropylene and 23 parts by weight of inorganic antibacterial component into a high-speed mixer and mix evenly. After granulation by twin-screw extrusion, the resulting product is antibacterial modified polypropylene masterbatch. The processing temperature of twin-screw extrusion is 180℃, the main screw speed is 300r / min, and the feed screw speed is 20r / min.

[0082] The preparation method of polypropylene nonwoven fabric is as follows:

[0083] Polypropylene and antibacterial modified polypropylene masterbatch were melt-blended at a weight ratio of 19:1, and then melt-blown to produce polypropylene nonwoven fabric. The temperature of the melt-blown spinning machine was 250℃, the receiving distance of the meltblown fabric was 300mm, the hot air pressure was 0.6MPa, and the winding speed was 5m / min.

[0084] S2. Polypropylene nonwoven fabric is treated with alkali and then treated with polydopamine. The result is called pretreated nonwoven fabric.

[0085] The alkaline treatment method is as follows:

[0086] The polypropylene nonwoven fabric was immersed in a 5% sodium hydroxide solution at 80°C for 2 hours, drained, and rinsed three times with pure water. Then it was immersed in a 1 mL / L acetic acid solution at room temperature for 10 minutes, rinsed four times with pure water, and dried at 58°C for 3 hours to complete the alkali treatment.

[0087] The method for polydopamine treatment is as follows:

[0088] Weigh 20g of dopamine hydrochloride and dissolve it in 10L of 0.01mol / L Tris buffer. Adjust the pH to 8.5 with 0.1mol / L hydrochloric acid and then immerse it in alkali-treated polypropylene nonwoven fabric. Shake in a 30℃ water bath for 24 hours, rinse 4 times with pure water, and dry at 33℃ for 7 hours to complete the polydopamine treatment.

[0089] S3. Immerse the pretreated nonwoven fabric in lysozyme solution, soak it at 37°C for 1 hour, rinse it with pure water and dry it. This is counted as one finishing process. After four finishing processes, the resulting biochemically modified antibacterial nonwoven fabric is obtained. The number of times to rinse with pure water and dry is three, and the drying temperature and time are 60°C and 1 hour, respectively.

[0090] The preparation method of the lysozyme solution is as follows:

[0091] Weigh 10g of thiourea dioxide and dissolve it in 5L of 0.01mol / L Tris buffer. Adjust the pH to 7.4 with 0.1mol / L hydrochloric acid, add 10g of lysozyme, and stir at 100r / min for 5min. The resulting solution is the lysozyme solution.

[0092] A biochemically modified antibacterial nonwoven fabric is prepared by a method for preparing a biochemically modified antibacterial nonwoven fabric.

[0093] Comparative Example 1

[0094] The biochemically modified antibacterial nonwoven fabric and its preparation method provided in this comparative example are largely the same as those in Example 1. The main difference is that the antibacterial modified polypropylene masterbatch in Example 1 is replaced with polypropylene masterbatch.

[0095] Comparative Example 2

[0096] The biochemically modified antibacterial nonwoven fabric and its preparation method provided in this comparative example are largely the same as those in Example 1. The main difference is that this comparative example 2 did not undergo the alkali treatment as in Example 1.

[0097] Comparative Example 3

[0098] The biochemically modified antibacterial nonwoven fabric and its preparation method provided in this comparative example are largely the same as those in Example 1. The main difference is that this comparative example 3 did not undergo the polydopamine treatment as in Example 1.

[0099] Performance testing

[0100] The biochemically modified antibacterial nonwoven fabrics prepared in Examples 1-3 and Comparative Examples 1-3 were labeled as Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2, and Comparative Example 3, respectively. The antibacterial properties of Examples 1-3 and Comparative Examples 1-3 were then tested according to the standard GB / T20944.3-2008. The tested bacteria were Escherichia coli, Pseudomonas, bacilli, and Staphylococcus aureus. The data obtained are recorded in the table below:

[0101]

[0102] The data in the table above shows that the biochemically modified antibacterial nonwoven fabrics prepared in Examples 1-3 have a higher antibacterial rate. This indicates that using the antibacterial modified polypropylene masterbatch as the base material in the preparation process of the biochemically modified antibacterial nonwoven fabric can achieve better antibacterial performance. It also shows that the nonwoven fabric can achieve better antibacterial effect after being treated with alkali and polydopamine and then immersed in lysozyme solution.

[0103] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0104] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for preparing a biochemically modified antibacterial nonwoven fabric, characterized in that, The preparation method of the biochemically modified antibacterial nonwoven fabric is as follows: S1. Polypropylene nonwoven fabric is made by melt-blowing antibacterial modified polypropylene masterbatch as raw material. S2. Polypropylene nonwoven fabric is treated with alkali and then treated with polydopamine. The result is called pretreated nonwoven fabric. S3. Immerse the pretreated nonwoven fabric in lysozyme solution, soak it at 37°C for 1 hour, rinse it with pure water and dry it. This is counted as one finishing process. The result after four finishing processes is the biochemically modified antibacterial nonwoven fabric. The preparation steps of the antibacterial modified polypropylene masterbatch in S1 are as follows: Step 1: Dissolve 2 parts by weight of silane coupling agent KH-570 in 500 parts by weight of ethanol aqueous solution, adjust the pH value to 4-5 with glacial acetic acid, stir and disperse, add 10 parts by weight of nano zinc oxide and 10 parts by weight of nano titanium dioxide, sonicate and then stir magnetically, filter and wash 3 times with anhydrous ethanol, dry and grind through a 200-mesh sieve. The resulting product is recorded as the inorganic antibacterial component. Step 2: Pour 80-90 parts by weight of polypropylene and 20-25 parts by weight of inorganic antibacterial component into a high-speed mixer and mix evenly. After granulation by twin-screw extrusion, the resulting product is antibacterial modified polypropylene masterbatch. The preparation method of lysozyme solution in S3 is as follows: Weigh 10g of thiourea dioxide and dissolve it in 5L of 0.01mol / L Tris buffer. Adjust the pH to 7.4 with 0.1mol / L hydrochloric acid, add 10g of lysozyme, and stir at 100r / min for 5min. The resulting solution is the lysozyme solution.

2. The method for preparing a biochemically modified antibacterial nonwoven fabric according to claim 1, characterized in that, In step 1, the volume fraction of the ethanol-water solution is 90%. The stirring and dispersion method is to stir at a speed of 300-500 r / min for 30 min. The ultrasonic treatment method is to ultrasonically disperse at a power of 200-300 W for 30 min. The magnetic stirring method is to stir at a speed of 500-600 r / min for 2-4 h. The drying treatment method is to dry at a temperature of 70-80℃ until constant weight.

3. The method for preparing a biochemically modified antibacterial nonwoven fabric according to claim 1, characterized in that, In step 2, the processing temperature of the twin-screw extrusion is 170-190℃, the main screw speed is 300r / min, and the feed screw speed is 20r / min.

4. The method for preparing a biochemically modified antibacterial nonwoven fabric according to claim 1, characterized in that, The preparation method of polypropylene nonwoven fabric in S1 is as follows: Polypropylene and antibacterial modified polypropylene masterbatch were melt-blended at a weight ratio of 19:1, and then melt-blown to produce polypropylene nonwoven fabric. The temperature of the melt-blown spinning machine was 240-260℃, the receiving distance of the meltblown fabric was 300mm, the hot air pressure was 0.6MPa, and the winding speed was 5m / min.

5. The method for preparing a biochemically modified antibacterial nonwoven fabric according to claim 1, characterized in that, The method for alkali treatment in S2 is as follows: The polypropylene nonwoven fabric is immersed in a 5% sodium hydroxide solution at 80℃ for 2 hours, drained, and rinsed 3 times with pure water. Then it is immersed in a 1 mL / L acetic acid solution at room temperature for 10 minutes, rinsed 3-5 times with pure water, and dried at 55-60℃ for 2-3 hours to complete the alkali treatment.

6. The method for preparing a biochemically modified antibacterial nonwoven fabric according to claim 1, characterized in that, The method for treating polydopamine in S2 is as follows: Weigh 20g of dopamine hydrochloride and dissolve it in 10L of 0.01mol / L Tris buffer. Adjust the pH to 8.5 with 0.1mol / L hydrochloric acid and then immerse it in alkali-treated polypropylene nonwoven fabric. Shake in a 30℃ water bath for 24 hours, rinse with pure water 3-5 times, and dry at 30-35℃ for 5-8 hours to complete the polydopamine treatment.

7. The method for preparing a biochemically modified antibacterial nonwoven fabric according to claim 1, characterized in that, In S3, the rinsing and drying process involves 2-3 rinses with pure water, and the drying temperature and time are 60℃ and 1 hour, respectively.

8. A biochemically modified antibacterial nonwoven fabric, characterized in that, It is prepared by the method for preparing a biochemically modified antibacterial nonwoven fabric according to any one of claims 1-7.

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