Longteng recovery paste as well as preparation method and application thereof

Abstract

The invention relates to the technical field of traditional Chinese medicine compositions, in particular to Longteng recovery paste as well as a preparation method and application thereof. Comprising the following components in parts by weight: 55-65 parts of astragalus membranaceus, 25-35 parts of caulis spatholobi, 25-35 parts of sargentgloryvine stem, 5-8 parts of peach kernel, 5-8 parts of safflower carthamus, 12-18 parts of leech, 12-18 parts of angelica sinensis, 20-25 parts of radix paeoniae alba, 12-18 parts of earthworm, 12-18 parts of fructus alpiniae oxyphyllae, 25-35 parts of radix rehmanniae preparata, 12-18 parts of ligusticum wallichii, 12-18 parts of radix paeoniae rubra, 25-35 parts of semen cuscutae, 12-18 parts of parasitic loranthus and 5-8 parts of elecampane. The traditional Chinese medicine has the pathogenesis characteristics of qi deficiency and blood stasis, and is rigorous in formula and reasonable in compatibility according to the treatment principle of tonifying primordial qi and promoting blood circulation to remove meridian obstruction.

Description

Technical Field

[0001] The present invention relates to the field of traditional Chinese medicine, and in particular to Longteng recovery ointment, a preparation method and application thereof. Background Art

[0002] Stroke is a common central nervous system disease. Its sequelae, primarily manifesting as hemiplegia, facial paralysis, slurred speech, and limb numbness, severely impact patients' quality of life. Currently, clinical treatment for stroke sequelae primarily relies on Western medicine rehabilitation, but this approach is limited in effectiveness and requires long-term adherence, leading to poor patient compliance.

[0003] Traditional Chinese Medicine has extensive experience and theoretical foundations in treating stroke sequelae. "Medical Errors Correction" explicitly states: "Hemiplegia and hemiplegia share the same underlying cause, yet various theories differ... I say: Vital energy is stored within the trachea and distributed throughout the body, with each side receiving half... Once vital energy is depleted, the meridians naturally become empty, and with these gaps, the energy inevitably flows to one side... Unable to move, unable to move, is called hemiplegia. Paralysis is the term for a disabled person."

[0004] While existing Chinese patent medicines for treating stroke sequelae, such as Buyang Huanwu Decoction and Sanqi Tongshu Capsules, have some efficacy, there is still a lack of targeted and effective preparations for stroke sequelae characterized by deficiency of vital energy and qi deficiency and blood stasis. Therefore, developing a Chinese herbal preparation specifically for these syndromes, which can both replenish vital energy and promote blood circulation and dredge meridians, has significant clinical and social significance. Summary of the Invention

[0005] The purpose of the present invention is to provide Longteng Recovery Ointment, its preparation method and application. The preparation targets the pathogenesis characteristics of deficiency of vital energy after stroke and concurrent qi deficiency and blood stasis syndrome, and effectively improves the sequelae of stroke such as facial paralysis, hemiplegia, drooling and the like through the therapeutic principles of replenishing vital energy and promoting blood circulation and unblocking meridians.

[0006] The technical solutions of the present invention are as follows:

[0007] Longteng Restoring Paste comprises the following components by weight: 55-65 parts of Astragalus, 25-35 parts of Millettia reticulata, 25-35 parts of Sargentodoxa chinensis, 5-8 parts of peach kernel, 5-8 parts of safflower, 12-18 parts of leech, 12-18 parts of angelica, 20-25 parts of white peony root, 12-18 parts of earthworm, 12-18 parts of Alpinia oxyphylla, 25-35 parts of Rehmannia glutinosa, 12-18 parts of Chuanxiong, 12-18 parts of red peony root, 25-35 parts of Cuscuta chinensis, 12-18 parts of mistletoe, and 5-8 parts of costus root.

[0008] The present invention also provides a method for preparing the Longteng Restoring Cream, comprising the following steps:

[0009] A. Raw Material Pretreatment: Remove impurities from each component medicinal material, wash and dry them; cut Astragalus, Angelica, White Peony Root, Red Peony Root, and Chuanxiong into 1-3 mm slices; cut Millettia Spatholobi and Sargentodoxae Caulis into 3-5 mm slices; crush Leech and pass through a 40-mesh sieve; crush the remaining medicinal materials and pass through a 20-mesh sieve;

[0010] B. Extraction: Group the processed herbs for extraction;

[0011] C. Combine the extracts: Combine the extracts from each group;

[0012] D. Concentration: Concentrate the combined extracts under reduced pressure;

[0013] E. Preparation: Add honey to the concentrate and mix well to obtain the Longteng recovery cream.

[0014] Preferably, the extraction in step B comprises:

[0015] B1. Water Extraction: Astragalus, Millettia reticulata, Sargentodoxae, Angelica sinensis, White Peony Root, Earthworm, Rehmannia glutinosa, Chuanxiong, Paeonia lactiflora, Cuscuta chinensis, and Morus alba were added to an extraction tank and soaked in 10 times the amount of water for 30 minutes. The mixture was heated to 90-95°C and refluxed for 2 hours. The mixture was filtered while hot. The residue was then extracted with 8 times the amount of water for 1.5 hours; then 6 times the amount of water for 1 hour. The filtrates were combined.

[0016] B2. Alcohol extraction: Peach kernel, safflower, Alpinia oxyphylla, and costus root were added to an extraction tank and soaked in 8 times the volume of 70% ethanol for 4 hours. The mixture was heated to 60-65°C and refluxed for 1.5 hours. The mixture was filtered while hot. The residue was then extracted with 6 times the volume of 70% ethanol for 1 hour. The two filtrates were combined.

[0017] B3. Leech extraction: Add leech powder to an extraction tank, add 12 times the amount of purified water, and extract at a constant temperature of 45-50°C for 4 hours while stirring. Filter and collect the filtrate.

[0018] Preferably, the pH value of the water extraction in step B1 is 6.5-7.0; the pH value of the leech extraction in step B3 is 6.0-7.0.

[0019] Preferably, the step B may further optionally include:

[0020] Ultrasonic-assisted extraction: Add the crushed earthworms and leeches into an ultrasonic extraction tank, add purified water, the solid-liquid ratio is 1:12, the ultrasonic power is 500W, the frequency is 40kHz, the temperature is 45℃, the time is 30 minutes, and the extract is filtered for later use.

[0021] Preferably, the step B may further optionally include:

[0022] Supercritical CO2 extraction: Put the powders of costus root, ligusticum chuanxiong and angelica sinensis into the extractor, extract for 2 hours at a pressure of 25 MPa, a temperature of 45°C and a synergist of 5% ethanol, and collect the extract.

[0023] Preferably, the conditions for the reduced pressure concentration in step D are: temperature 60-65°C, vacuum degree -0.08 MPa, and the concentration end point is a relative density of 1.25-1.30 (60°C).

[0024] Preferably, the amount of honey added in step E is 30% of the weight of the concentrate, the mixing temperature is 50-55° C., the homogenization speed is 60-80 rpm, and the homogenization time is 30 minutes.

[0025] Preferably, the method further includes filling and sterilizing steps: filling the ointment into a sterilized glass bottle in a prescribed amount of 200ml±5ml, tightening the bottle cap, heat sterilizing at 100°C for 30 minutes, and labeling after cooling.

[0026] The present invention also discloses the use of Longteng Restoring Ointment in the preparation of a medicine for treating stroke sequelae, wherein the stroke sequelae include facial paralysis, hemiplegia, drooling at the corners of the mouth, dry stool, frequent urination, incontinence, and dizziness, vomiting, and numbness and discomfort in the face caused by a minor stroke due to insufficient vital energy.

[0027] Beneficial effects of the present invention:

[0028] 1. The Longteng Restoring Paste of the present invention fully combines traditional Chinese medicine theory with modern pharmacological research. It targets the pathogenesis of stroke sequelae, namely "vital energy deficiency and combined with qi deficiency and blood stasis". It adopts the treatment principle of "tonifying vital energy, activating blood circulation and unblocking meridians" with rigorous formula and reasonable compatibility.

[0029] 2. The present invention adopts a step-by-step extraction preparation process. According to the characteristics of the active ingredients of different medicinal materials, water extraction, alcohol extraction and special extraction methods are used respectively, which effectively improves the extraction rate of each active ingredient and ensures the stability of product quality.

[0030] 3. The Longteng Restoring Ointment of the present invention has shown good therapeutic effects in clinical applications. It can effectively improve symptoms such as facial paralysis, hemiplegia, and drooling in patients with stroke sequelae, and improve the quality of life of patients. DETAILED DESCRIPTION

[0031] 1. Raw material composition and pharmacological effects

[0032] The Longteng Restoring Paste of the present invention is composed of 16 Chinese medicinal herbs, and the pharmacological effects of each component are as follows:

[0033] 1. Astragalus (55-65 parts): This is the main ingredient in this prescription, known for its benefits of tonifying Qi and raising Yang, strengthening the exterior and stopping perspiration, promoting diuresis and reducing swelling, and promoting fluid production and nourishing blood. Modern pharmacological research has shown that Astragalus contains active ingredients such as astragalus polysaccharides, astragalus saponins, and flavonoids. Astragalus polysaccharides can enhance immunity, provide antioxidant protection, and protect the liver; astragalus saponins exert antibacterial and anti-inflammatory effects by regulating signaling pathways and protein expression; and flavonoids have antioxidant properties. The "Compendium of Materia Medica" states: "Astragalus nourishes the body, replenishing vital Qi and harmonizing the meridians." This prescription utilizes a heavy dose of raw Astragalus to raise Yang Qi and draw out vital Qi from the kidneys. To prevent excessive growth, herbs such as Cuscuta chinensis and Morus alba are incorporated into the prescription, primarily to replenish vital Qi and secondly to prevent excessive growth from the Astragalus.

[0034] 2. Millettia reticulata and Sargentodoxae (25-35 parts each): These are the main ingredients in this prescription, known for their efficacy in promoting blood circulation, removing blood stasis, and promoting blood circulation. Millettia reticulata contains flavonoids, triterpenes, and polysaccharides, which primarily promote blood circulation, promote blood circulation, and strengthen bones and muscles. Sargentodoxae contains lignans, polyphenols, and triterpenes, which have the effects of clearing heat and detoxifying, promoting blood circulation, and relieving pain. The combination of these two herbs can enhance the effects of promoting blood circulation, improving microcirculation, and increasing cerebral blood flow.

[0035] 3. Peach kernel (5-8 parts) and safflower (5-8 parts): These are the main components of Taohong Siwu Decoction, known for promoting blood circulation, removing blood stasis, and relieving pain. Peach kernel contains amygdalin, fatty oils, and peach kernel polysaccharides, while safflower contains safflower yellow pigment, safflower glycosides, and volatile oils. "Compendium of Materia Medica Chongyuan" records: "Peach kernel is initially green and then purple, and its flavor is sweet and sour, reflecting the wood element. Its kernel also soothes the liver, treating blood stasis and blood stasis, and dispersing liver qi." "Compendium of Materia Medica Huiyan" states: "Safflower is a blood-clearing, blood-activating, blood-regulating, and blood-regulating herbal medicine." Combined use of these two herbs has potent anticoagulant and antiplatelet effects, potentially improving microcirculation in areas of cerebral ischemia.

[0036] 4. Leeches (12-18 parts): Containing hirudin and polypeptides, they have anticoagulant, thrombolytic, and microcirculatory benefits. "Compendium of Materia Medica" records: "Leech are aquatic animals with a salty and bitter flavor, representing the yang within the yin. Salty and bitter flavors move the blood, so they are effective in expelling blood stasis and relieving amenorrhea." Modern pharmacological research shows that hirudin can specifically break down fibrin and dissolve blood clots, making it particularly suitable for treating the sequelae of blood stasis-type stroke.

[0037] 5. Angelica (12-18 parts), Chuanxiong (12-18 parts), White Peony Root (20-25 parts), and Red Peony Root (12-18 parts): All are ingredients in Siwu Decoction, known for their effects on nourishing and activating blood circulation, regulating menstruation, and alleviating pain. Angelica contains ferulic acid, ligustilide, and polysaccharides; Chuanxiong contains ferulic acid, ligustrazine, and volatile oils; White Peony Root and Red Peony Root contain paeoniflorin and paeoniflorin. The Compendium of Materia Medica states: "The Qi of the heart and kidney intersect upwards, each with its own destination. Therefore, Angelica can both invigorate blood circulation and dredge meridians, connecting the heart and kidney." "The heart governs blood, and the liver stores blood. Peony, with its wood Qi, treats the liver, while with its fire Qi, treats the heart, thus relieving blood stasis." "Chuanxiong is primarily used to treat headaches caused by strokes affecting the brain. Chuanxiong, with its metal Qi, treats wind, and its upward nature affects the brain." Combined use of these herbs can effectively improve blood circulation, increase cerebral blood flow, and promote neural repair.

[0038] 6. Earthworm (12-18 servings): Contains earthworm protein, peptides, and polysaccharides, which have been shown to promote blood circulation, lower blood pressure, and fight inflammation. Compendium of Materia Medica states: "Its nature is cold and downward... Therefore, it promotes urination, treats foot ailments, and unclogs the meridians." Modern research demonstrates that earthworms activate meridians and collaterals, circulate throughout the body, and guide the effects of other medicinal herbs.

[0039] 7. Alpinia oxyphylla (12-18 parts): Contains volatile oils, flavonoids, and alkaloids, and has the effects of tonifying the kidneys, strengthening essence, and warming and dispelling cold and dampness. "Compendium of Materia Medica" records: "Alpinia oxyphylla – treats spermatorrhea, loose stools, and dribbling urine... replenishes deficiency, benefits the triple energizer, and regulates various qi." In this recipe, Alpinia oxyphylla is combined with Cuscuta seed and Morus alba to replenish vital energy while preventing excessive growth of Astragalus root.

[0040] 8. Rehmannia glutinosa (25-35 parts): Contains rehmannia glycosides, polysaccharides, and iridoids, which have blood-tonifying, yin-nourishing, and antioxidant properties. The Compendium of Materia Medica states: "Rehmannia glutinosa is yellow in color, sweet in flavor, and cold in nature. It possesses the specialized essence of Taiyin Zhongtu (earth element) and the qi transformation of Shaoyin cold water. It primarily treats injuries to the middle part of the body. Its sweet flavor and moist texture replenish the essence of the middle burner." Rehmannia glutinosa, combined with angelica and white peony root, enhances its blood-tonifying and nourishing effects.

[0041] 9. Cuscuta seeds (25-35 parts) and mistletoe (12-18 parts): Cuscuta seeds contain cuscuta sterols, flavonoids, and polysaccharides; mistletoe contains flavonoids, alkaloids, and polysaccharides. Shennong's Herbal Classic states: "Cuscuta seeds replenish deficiencies and strengthen Qi and strength." Mingyi Bielu states: "Mistletoe…relieves internal injuries and deficiencies..." Combining these two herbs can not only replenish vital energy but also prevent excessive growth of Astragalus, which can further deplete kidney Qi.

[0042] 10. Costus root (5-8 parts): Contains volatile oils and sesquiterpene lactones, which promote qi circulation, relieve pain, and promote communication between the upper and lower parts. The "Compendium of Materia Medica" records: "The scent of costus root is fragrant, and its number is five. Its pungent and warm aroma reaches up to the nine heavens. It inherits the qi of heaven and earth from the hands and feet, and controls the interaction of heaven and earth qi, connecting the upper and lower parts." In this prescription, costus root not only nourishes the kidneys and strengthens willpower, but also promotes communication between the upper and lower parts, ensuring that the entire formula is nourishing without stagnation.

[0043] The Longteng Restoring Cream of the present invention forms an overall therapeutic effect of "replenishing vital energy, promoting blood circulation and unblocking meridians" through the rational combination of the above components, and has a significant improvement effect on the sequelae of stroke.

[0044] 2. Preparation Method

[0045] The method for preparing the Longteng Restoring Cream of the present invention comprises the following specific steps:

[0046] A. Raw material pretreatment: Raw material pretreatment is crucial to the quality of the final product. First, high-quality medicinal materials must be selected in strict accordance with the standards of the Chinese Pharmacopoeia. Preferably, Astragalus should be selected from Astragalus membranaceus (produced in Minxian County, Gansu Province) that has been grown for more than three years, with a firm texture, yellow-white color, and a strong fibrous cross-section. For Millettia reticulata, the ones with 3-8 eccentric rings on the cross-section and resinous secretions that are reddish-brown to black-brown should be selected. For Sargentophyllum nobile, the ones with uniform strips, thick stems, and brown-red color should be selected. For Angelica sinensis, the ones with a soft texture, oily cross-section, and a strong aroma should be selected.

[0047] The selected herbs are accurately weighed according to the recipe's weight, removed of impurities, washed, and air-dried. Then, different preliminary processing steps are performed based on the herbs' characteristics: Astragalus, Angelica, White Peony Root, Red Peony Root, and Chuanxiong are sliced into 1-3 mm slices; Millettia Reticulata and Sargentodoxae Caulis are sliced into 3-5 mm slices; Leech is pulverized and passed through a 40-mesh sieve; and the remaining herbs (i.e., Alpinia oxyphylla, Rehmannia glutinosa, Cuscuta chinensis, Morus alba, Costus root, Peach Kernel, and Carthamus tinctorius) are pulverized and passed through a 20-mesh sieve. This processing method fully considers the tissue structural characteristics of each herb and facilitates the extraction of active ingredients in the subsequent extraction process.

[0048] B. Extraction: Taking into account the differences in the physical and chemical properties of the active ingredients in different medicinal materials, the present invention adopts a step-by-step extraction method to maximize the extraction and retention of the active ingredients of each medicinal material.

[0049] B1. Water Extraction: Add Astragalus, Millettia reticulata, Sargentodoxae chinensis, Chinese Angelica sinensis, White Peony Root, Earthworm, Rehmannia glutinosa, Chuanxiong, Paeonia lactiflora, Cuscuta chinensis, and Morus alba to the extraction tank. These herbs primarily contain water-soluble ingredients, such as Astragalus polysaccharides, Chinese Angelica polysaccharides, and Earthworm protein.

[0050] Add 10 times the amount of purified water (based on the total weight of the medicinal materials) to the extraction tank and soak for 30 minutes to allow the medicinal materials to fully absorb the water and expand. During the soaking process, the pH should be controlled between 6.5-7.0, as this pH range is particularly beneficial for the extraction of polysaccharides. After soaking, heat to 90-95°C and reflux for 2 hours. This temperature range effectively extracts polysaccharides while preventing damage to heat-sensitive components. After extraction, filter while hot and collect the filtrate.

[0051] The residue was extracted a second time, adding 8 times the amount of purified water and extracting under the same conditions for 1.5 hours. A third extraction was then performed, adding 6 times the amount of purified water and extracting for 1 hour. Finally, the three filtrates were combined to obtain the aqueous extract. Multiple extractions ensured that the water-soluble components in the medicinal material were fully extracted.

[0052] B2. Alcohol Extraction: Add peach kernel, safflower, Alpinia oxyphylla kernel, and costus root to another extraction tank. These herbs primarily contain fat-soluble or alcohol-soluble components, such as amygdalin and fatty oils in peach kernel, safflower yellow pigment in safflower, and volatile oils in Alpinia oxyphylla kernel and costus root.

[0053] Add 8 times the volume of 70% ethanol (based on the total weight of the medicinal material) to the extraction tank and soak for 4 hours. The ethanol concentration is selected based on the polarity of the solvent and the compatibility of the target components. After soaking, heat to 60-65°C and reflux extract for 1.5 hours. This temperature range effectively extracts the target components while maximizing the retention of volatile components. After extraction, filter while hot and collect the filtrate.

[0054] The filter residue was extracted a second time by adding 6 times the amount of 70% ethanol and extracting under the same conditions for 1 hour. Finally, the two filtrates were combined to obtain the alcohol extract.

[0055] B3. Leech Extraction: Leeches contain specialized proteins and enzymes that require separate extraction to maintain their activity. Add leech powder to the third extraction tank and add 12 times the volume of purified water, controlling the pH between 6.0 and 7.0. Incubate the extract at a constant temperature of 45-50°C for four hours with gentle stirring. This gentle extraction condition maximizes the activity of proteins such as hirudin. After extraction, filter and collect the filtrate to obtain the leech extract.

[0056] Preferably, the present invention can also use modern extraction technology to extract specific components to further improve the extraction efficiency and retain the integrity of the active ingredients.

[0057] For example, ultrasonic-assisted extraction can be used: Crushed earthworms and leeches are placed in an ultrasonic extraction tank, along with purified water, at a solid-to-liquid ratio of 1:12. Extraction is performed at 500W, 40kHz, and 45°C for 30 minutes, followed by filtration. Ultrasonic waves, through cavitation, disrupt cell wall structure, promoting the rapid release of active ingredients and improving extraction efficiency.

[0058] Alternatively, supercritical CO2 extraction can be used: powdered costus root, chuanxiong rhizome, and angelica root are placed in an extractor and extracted for two hours at a pressure of 25 MPa, a temperature of 45°C, and a synergist of 5% ethanol. The extract is then collected. Supercritical CO2 extraction is highly effective for extracting volatile oils, preserving the integrity of the volatile components and eliminating solvent residue.

[0059] C. Combine the extracts: Combine the aqueous extract, alcohol extract, and leech extract obtained in steps B1, B2, and B3. If ultrasonic-assisted extraction or supercritical CO2 extraction was used, these extracts should also be combined. Care should be taken to control the temperature during the combination process, preferably at 30-40°C to prevent damage to heat-sensitive components.

[0060] D. Concentration: Place the combined extracts into a vacuum concentrator and concentrate under reduced pressure at 60-65°C and a vacuum of -0.08 MPa. Closely monitor the density of the concentrate during the concentration process. Stop concentrating when the relative density (measured at 60°C) reaches 1.25-1.30. Vacuum concentration removes the solvent at a lower temperature, maximizing the retention of heat-sensitive and volatile components.

[0061] E. Preparation: Adjust the temperature of the concentrate to 50-55°C, add honey (30% by weight of the concentrate), and homogenize at 60-80 rpm for 30 minutes. Then cool to room temperature to obtain Longteng Restoring Paste. The addition of honey not only improves the taste but also enhances the stability of the preparation, extending its shelf life. Furthermore, honey itself has the properties of nourishing the spleen and stomach, moistening the lungs, and relieving coughs, synergistically enhancing the efficacy of this prescription.

[0062] F. Filling and Sterilization: Fill the prepared Longteng Restoring Ointment into a sterilized glass bottle at a prescribed volume of 200ml ± 5ml. Tighten the cap and sterilize at 100°C for 30 minutes. After cooling, label the bottle. The filling and sterilization process should be performed in a clean environment to ensure that the microbiological quality of the product meets the requirements of the Chinese Pharmacopoeia.

[0063] 3. Examples

[0064] Example 1: Basic Formula Longteng Recovery Cream

[0065] Prepared according to the following weight parts: 60 parts of Astragalus, 30 parts of Millettia reticulata, 30 parts of Sargentodoxa chinensis, 6 parts of peach kernel, 6 parts of safflower, 15 parts of leech, 15 parts of angelica, 23 parts of white peony root, 15 parts of earthworm, 15 parts of Alpinia oxyphylla, 30 parts of Rehmannia glutinosa, 15 parts of Chuanxiong, 15 parts of red peony root, 30 parts of Cuscuta seeds, 15 parts of mistletoe, and 6 parts of costus root.

[0066] Preparation method:

[0067] First, pre-treat the raw materials. Remove impurities from each of the above herbs, wash and dry them. Cut the Astragalus, Angelica, White Peony Root, Red Peony Root, and Chuanxiong into 2 mm slices. Cut Millettia Spatholobi and Sargentodoxae into 4 mm slices. Crush the leech through a 40-mesh sieve. Crush the Alpinia oxyphylla, Rehmannia glutinosa, Cuscuta chinensis, Morus alba, Costus root, Peach kernel, and Safflower through a 20-mesh sieve.

[0068] Next, a step-by-step extraction process was performed. For the aqueous extraction, 263 parts of Astragalus, Millettia reticulata, Sargentodoxae, Angelica sinensis, White Peony Root, Earthworm, Rehmannia glutinosa, Chuanxiong, Paeonia lactiflora, Cuscuta chinensis, and Morus alba were added to an extraction tank. 2630 parts (10 times the volume) of purified water were added and the mixture was soaked for 30 minutes. The mixture was then heated to 92°C and refluxed for 2 hours, with the pH controlled at 6.8. The mixture was then filtered while hot. 2104 parts (8 times the volume) of water were added to the residue and extracted under the same conditions for 1.5 hours. Another 1578 parts (6 times the volume) of water were added and extracted for 1 hour. The three filtrates were combined to obtain the aqueous extract. For the alcohol extraction, add 42 parts of peach kernel, safflower, alpinia oxyphylla, and costus root to an extraction tank, add 336 parts (8 times the volume) of 70% ethanol, soak for 4 hours, heat to 62°C, reflux extract for 1.5 hours, and filter while hot. Add 252 parts (6 times the volume) of 70% ethanol to the residue and extract for 1 hour. Combine the two filtrates to obtain the alcohol extract. For the leech extraction, add 15 parts of leech powder to an extraction tank, add 180 parts (12 times the volume) of purified water, and extract at 48°C for 4 hours, controlling the pH to 6.5. Stir continuously during the extraction process. Filter and collect the filtrate to obtain the leech extract.

[0069] Then, the water extract, alcohol extract and leech extract were combined and concentrated under reduced pressure at 62°C and a vacuum degree of -0.08 MPa. Concentration was stopped when the relative density of the concentrate reached 1.28 (measured at 60°C).

[0070] Finally, the concentrate was cooled to 53°C, and honey (30% by weight) was added. The mixture was homogenized and mixed at 70 rpm for 30 minutes. The mixture was then cooled to room temperature to obtain Longteng Restoring Cream. The prepared Longteng Restoring Cream was then filled into sterilized glass bottles (200 ml each), capped, and heat-sterilized at 100°C for 30 minutes. After cooling, the mixture was labeled.

[0071] In this embodiment, astragalus is used as the main drug to replenish vital energy, Millettia reticulata and Sargentodoxae synergistically promote blood circulation and dredge the meridians, peach kernel and safflower remove blood stasis and dredge the meridians, leech dissolves blood clots, angelica, Chuanxiong, white peony root, and red peony root replenish blood and promote blood circulation, earthworm dredges the meridians and guides the flow, Alpinia oxyphylla, Cuscuta chinensis, and Morus alba replenish the kidneys and strengthen essence, Rehmannia glutinosa nourishes yin and replenishes blood, and costus root promotes qi circulation and guides qi. The combination of these drugs has the effects of replenishing vital energy, promoting blood circulation and dredge the meridians, and has a good therapeutic effect on the vital energy deficiency and qi deficiency and blood stasis syndrome of stroke sequelae.

[0072] Example 2: High-dose Astragalus Longteng Restoring Cream

[0073] Prepared according to the following weight parts: 65 parts of Astragalus, 30 parts of Millettia reticulata, 30 parts of Sargentodoxa chinensis, 6 parts of peach kernel, 6 parts of safflower, 15 parts of leech, 15 parts of angelica, 23 parts of white peony root, 15 parts of earthworm, 15 parts of Alpinia oxyphylla, 30 parts of Rehmannia glutinosa, 15 parts of Chuanxiong, 15 parts of red peony root, 30 parts of Cuscuta seeds, 15 parts of mistletoe, and 6 parts of costus root.

[0074] The preparation method is substantially the same as that of Example 1, except that the amount of Astragalus is increased to 65 parts, and the amount of water used for extraction is correspondingly increased. In this embodiment, increasing the amount of Astragalus can enhance the effect of replenishing vital energy, and is suitable for patients with severe post-stroke sequelae due to vital energy deficiency.

[0075] Example 3: Enhanced Blood Activating and Collateral Unblocking Longteng Recovery Cream

[0076] Prepared according to the following weight parts: 55 parts of Astragalus, 35 parts of Millettia reticulata, 35 parts of Sargentodoxae dasyphylla, 8 parts of peach kernel, 8 parts of safflower, 18 parts of leech, 15 parts of angelica, 23 parts of white peony root, 15 parts of earthworm, 15 parts of Alpinia oxyphylla, 30 parts of Rehmannia glutinosa, 18 parts of Chuanxiong, 15 parts of red peony root, 30 parts of Cuscuta seed, 15 parts of mistletoe, and 6 parts of costus root.

[0077] The preparation method is essentially the same as Example 1, except that the amounts of Millettia repens, Sargentodoxae dahuricae, Semen persicae, Carthamus tinctorius, Hirudo, and Rhizoma Chuanxiong are increased. This example enhances the efficacy of promoting blood circulation and unblocking meridians and is suitable for patients with post-stroke symptoms exhibiting significant blood stasis. During the preparation process, the total amount of medicinal materials in the water extract portion is increased to 274 parts. Accordingly, the water consumption for the first extraction is 2740 parts, the second extraction is 2192 parts, and the third extraction is 1644 parts.

[0078] Example 4: Ultrasonic-assisted extraction of Longteng recovery cream

[0079] The component ratios were the same as in Example 1, except that an ultrasonic-assisted extraction step was added to the extraction process. Specifically, in the water extraction step, astragalus, Millettia reticulata, Sargentodoxae chinensis, Chinese Angelica sinensis, Paeonia lactiflora, Pheretima chinensis, Rehmannia glutinosa, Ligusticum chuanxiong, Paeonia lactiflora, Cuscuta chinensis, and Morus alba were added to an extraction tank and first ultrasonically treated (ultrasonic power 500 W, frequency 40 kHz, temperature 45°C, time 30 minutes), followed by conventional extraction according to the method of Example 1.

[0080] Ultrasonic-assisted extraction (using the same parameters as above) is also used for leech extraction, effectively increasing the extraction rate of macromolecular compounds such as hirudin. Experimental results show that ultrasonic-assisted extraction can increase the extraction rate of hirudin by approximately 25% and that of earthworm protein by approximately 20%.

[0081] Example 5: Supercritical CO2 extraction of Longteng recovery cream

[0082] The component ratios were the same as in Example 1, except that a supercritical CO2 extraction step was added to the preparation process. Specifically, the powders of costus root, ligusticum chuanxiong, and angelica were each subjected to supercritical CO2 extraction (pressure 25 MPa, temperature 45°C, 5% ethanol as a synergist, for 2 hours), and the extracts were collected. Conventional extraction was then performed according to the method of Example 1, and the supercritical CO2 extracts were combined with the other extracts.

[0083] Supercritical CO2 extraction technology can effectively extract the volatile oil components from costus root, chuanxiong rhizome, and angelica root, while preserving their intact structure, enhancing the aroma and efficacy of the preparation. Experimental results show that the volatile oil content extracted using this method is approximately 30% higher than that extracted using conventional methods.

[0084] Example 6: Low-dose medicinal material Longteng recovery ointment

[0085] Prepared according to the following weight parts: 55 parts of Astragalus, 25 parts of Millettia reticulata, 25 parts of Sargentodoxa chinensis, 5 parts of peach kernel, 5 parts of safflower, 12 parts of leech, 12 parts of angelica, 20 parts of white peony root, 12 parts of earthworm, 12 parts of Alpinia oxyphylla, 25 parts of Rehmannia glutinosa, 12 parts of Chuanxiong, 12 parts of red peony root, 25 parts of Cuscuta chinensis, 12 parts of mistletoe, and 5 parts of costus root.

[0086] The preparation method is basically the same as that of Example 1, except that the dosage of each medicinal material is adjusted to the lower limit. This example represents the basic formula of Longteng Recovery Ointment, which is suitable for patients with mild stroke sequelae or patients who are sensitive to traditional Chinese medicine.

[0087] Example 7: Longteng Recovery Cream with Adjusted Extraction Parameters

[0088] The composition ratio was the same as in Example 1, except that the extraction parameters were adjusted. For the water extraction, the temperature was adjusted to 95°C, and the extraction times were 2.5 hours, 2 hours, and 1.5 hours, respectively. For the alcohol extraction, the temperature was adjusted to 65°C, and the extraction times were 2 hours and 1.5 hours, respectively. For the leech extraction, the temperature was adjusted to 50°C, and the extraction time was extended to 5 hours.

[0089] Adjusting the extraction parameters can further improve the extraction rate of active ingredients. Experimental results show that the adjusted extraction process can increase the extraction rate of astragalus polysaccharides by about 15%, the extraction rate of paeoniflorin by about 12%, and the extraction rate of hirudin by about 18%.

[0090] Example 8: Microwave-assisted extraction of Longteng recovery ointment

[0091] The composition ratio is the same as that of Example 1, but a microwave-assisted extraction step is added to the preparation process. Specifically, microwave-assisted extraction (microwave power 600W, intermittent irradiation to avoid local overheating) is used for insoluble components such as Cuscuta seeds and Morus alba.

[0092] Microwave-assisted extraction can increase the extraction efficiency of flavonoids and saponins by approximately 20%. Furthermore, microwave technology can shorten extraction time, reduce energy consumption, and improve production efficiency.

[0093] In order to verify the technical effect of the present invention, the following comparative experiments were designed:

[0094] Comparative Example 1: Control formula lacking invigorating vital energy drugs

[0095] The following ingredients were prepared by weight: 30 parts of Millettia reticulata, 30 parts of Sargentodoxae dahuricae, 6 parts of peach kernel, 6 parts of safflower, 15 parts of leech, 15 parts of angelica sinensis, 23 parts of white peony root, 15 parts of earthworm, 15 parts of Chuanxiong, 15 parts of red peony root, and 6 parts of costus root. Compared with Example 1, the ingredients that replenish vital energy, such as astragalus, Alpinia oxyphylla, Rehmannia glutinosa, Cuscuta australis, and Morus alba, were omitted.

[0096] The preparation method is basically the same as that of Example 1, but does not contain the above-mentioned removed medicinal materials. This comparative example mainly embodies the effect of promoting blood circulation and unblocking meridians, but lacks the effect of replenishing vital energy.

[0097] Comparative Example 2: Control formula lacking blood circulation promoting and collateral dredging drugs

[0098] The following formula was prepared by weight: 60 parts of Astragalus, 15 parts of Angelica, 23 parts of White Peony Root, 15 parts of Alpinia oxyphylla, 30 parts of Rehmannia glutinosa, 30 parts of Cuscuta chinensis, 15 parts of Morus alba, and 6 parts of Aucklandia lappa. Compared with Example 1, the ingredients of Millettia reticulata, Sargentodoxae spatholobi, Semen persicae, Carthamus tinctorius, Hirudo, Pheretima chinensis, Rhizoma Chuanxiong, and Paeonia lactiflora were omitted as they promote blood circulation and dredge collaterals.

[0099] The preparation method is basically the same as that of Example 1, but does not contain the above-mentioned removed medicinal materials. This comparative example mainly reflects the effect of replenishing vital energy, but lacks the effect of promoting blood circulation and unblocking meridians.

[0100] Comparative Example 3: Traditional extraction process control

[0101] The component ratio is the same as that in Example 1, but a traditional one-step extraction process is adopted, that is, all the medicinal materials are mixed together, 10 times the amount of water is added, and decocted 3 times (the first time is 2 hours, the second time is 1.5 hours, and the third time is 1 hour). The decoctions are combined and concentrated to make a paste.

[0102] This comparative example adopts the traditional Chinese medicine decoction and extraction process, does not classify and extract medicinal materials with different characteristics, and does not adopt modern extraction technology.

[0103] Comparative Example 4: Control Formula without Honey Addition

[0104] The component ratio is the same as that in Example 1, and the preparation method is also basically the same, but no honey is added in the preparation step, and the concentrated liquid is directly dried into a dry paste.

[0105] This comparative example is intended to verify the effect of honey addition on the stability and effectiveness of the formulation.

[0106] Comparative Example 5: Controls with different extraction solvents

[0107] The component ratios were the same as in Example 1, but the solvent in all extraction steps was uniformly changed to 50% ethanol, and three extractions were performed (the extraction time was the same as in Example 1).

[0108] This comparative example was designed to verify the effects of step-by-step extraction and solvent selection on the extraction efficiency of the active ingredient.

[0109] In order to evaluate the efficacy of Longteng Recovery Ointment, the following evaluation experiments were conducted:

[0110] Experiment 1: Determination of Astragalus Polysaccharide Content

[0111] The phenol-sulfuric acid method was used to determine the content of astragalus polysaccharides in each example and comparative example. The specific method is as follows: 0.5 g of sample was taken, accurately weighed, and the astragalus polysaccharide determination method in 2341 of the General Rules of Part IV of the 2020 edition of the Chinese Pharmacopoeia was followed. The results are shown in Table 1.

[0112] Experiment 2: Determination of ferulic acid content

[0113] The ferulic acid content in each example and comparative example was determined using high-performance liquid chromatography. Chromatographic conditions: Agilent ZORBAX SB-C18 column (4.6 mm × 250 mm, 5 μm); mobile phase: acetonitrile-0.1% aqueous phosphoric acid (25:75); flow rate: 1.0 mL / min; column temperature: 30°C; detection wavelength: 320 nm; injection volume: 10 μL. The results are shown in Table 1.

[0114] Experiment 3: Hirudin activity assay

[0115] The hirudin activity in each example and comparative example was determined using the fibrin plate method. Specifically, a predetermined amount of sample was added to an agar plate containing standard fibrin, incubated at 37°C for 24 hours, and the diameter of the dissolved ring was measured to calculate the hirudin activity. The results are shown in Table 1.

[0116] Experiment 4: In vitro antiplatelet aggregation experiment

[0117] Venous blood was collected from healthy volunteers to prepare platelet-rich plasma (PRP), and the inhibitory effects of the Examples and Comparative Examples on adenosine diphosphate (ADP)-induced platelet aggregation were determined using turbidimetry. The results are shown in Table 1.

[0118] Experiment 5: Treatment experiment of acute cerebral ischemia rat model

[0119] A middle cerebral artery occlusion (MCAO) model was established in rats using the suture embolization method. The rats were randomly divided into a model group, a positive control group (nimodipine), groups of Examples 1-8, and groups of Comparative Examples 1-5, with 10 rats in each group. Seven days after administration, the therapeutic efficacy was evaluated using neurological deficit scoring, cerebral infarction volume measurement, and brain histopathological examination. The results are shown in Table 2.

[0120] Table 1: Chemical composition content and in vitro activity of various examples and comparative examples

[0121] sample Astragalus polysaccharide content (%) Ferulic acid content (%) Hirudin activity (U / g) Antiplatelet aggregation inhibition rate (%) Example 1 2.8 0.18 9500 62.5 Example 2 3.2 0.17 9200 60.8 Example 3 2.6 0.21 11200 68.3 Example 4 3 0.22 12500 65.2 Example 5 2.9 0.25 10800 67.4 Example 6 2.5 0.15 8200 58.6 Example 7 3.1 0.2 10500 63.8 Example 8 3 0.23 11000 66.1 Comparative Example 1 0.2 0.19 9800 65.3 Comparative Example 2 2.9 0.08 1200 15.2 Comparative Example 3 1.8 0.12 6500 45.7 Comparative Example 4 2.7 0.17 9200 60.5 Comparative Example 5 2 0.14 7800 50.2

[0122] Table 2: Therapeutic effects of various embodiments and comparative examples in acute cerebral ischemia rat model

[0123] Group Neurological impairment score (points) Reduction rate of cerebral infarction volume (%) Brain tissue SOD activity (U / mg protein) Brain tissue MDA content (nmol / mg protein) Model Group 3.2±0.4 - 62.5±5.8 3.8±0.5 Positive control group 2.1±0.3* 35.6±4.2* 85.3±7.2* 2.2±0.3* Example 1 1.8±0.2** 42.5±4.5** 95.2±8.5** 1.9±0.2** Example 2 1.6±0.2** 45.8±5.0** 98.6±9.0** 1.7±0.2** Example 3 1.7±0.3** 44.2±4.8** 96.5±8.8** 1.8±0.3** Example 4 1.5±0.2** 48.3±5.2** 102.3±9.5** 1.6±0.2** Example 5 1.6±0.3** 46.7±5.0** 100.5±9.2** 1.7±0.3** Example 6 2.0±0.3* 38.9±4.3* 90.6±8.0* 2.1±0.3* Example 7 1.7±0.2** 45.1±4.9** 97.8±8.9** 1.8±0.2** Example 8 1.6±0.2** 47.2±5.1** 101.2±9.3** 1.7±0.2** Comparative Example 1 2.6±0.3 22.5±2.5 75.2±6.8 2.8±0.4 Comparative Example 2 2.8±0.4 18.3±2.0 70.6±6.5 3.0±0.4 Comparative Example 3 2.4±0.3* 28.7±3.2* 80.3±7.5* 2.5±0.3* Comparative Example 4 1.9±0.3* 40.8±4.5* 93.5±8.5* 2.0±0.3* Comparative Example 5 2.3±0.3* 32.5±3.6* 83.7±7.8* 2.3±0.3*

[0124] Note: Compared with the model group, * p<0.05, p<0.01

[0125] From the experimental results in Table 1 and Table 2, we can see that:

[0126] 1. Active Ingredient Content: The astragalus polysaccharide content in Examples 1-8 was all above 2.5%, the ferulic acid content was all above 0.15%, and the hirudin activity was all above 8000 U / g, significantly higher than that in Comparative Examples 3 and 5. This demonstrates that the step-by-step extraction process and modern extraction technology of the present invention can effectively improve the extraction rate of various active ingredients. In particular, Example 4 (ultrasound-assisted extraction) and Example 5 (supercritical CO2 extraction) achieved higher active ingredient content and activity, confirming the superiority of modern extraction technology.

[0127] 2. Antiplatelet Aggregation Effect: Examples 1-8 all exhibited significant inhibition of ADP-induced platelet aggregation, with inhibition rates exceeding 58.6%. Examples 3, 4, 5, and 8 exhibited even higher inhibition rates, which is attributed to the dosage of the blood-activating and collateral-draining agents and the application of modern extraction technology. In contrast, the inhibition rate of Comparative Example 2 (lacking the blood-activating and collateral-draining agents) was only 15.2%, significantly lower than that of the other samples, demonstrating the importance of these blood-activating and collateral-draining agents in this recipe.

[0128] 3. Neuroprotective Effect: In a rat model of acute cerebral ischemia, Examples 1-8 significantly improved neurological deficit scores, reduced infarct volume, increased brain tissue SOD activity, and decreased MDA content, outperforming the positive control drug, nimodipine. In particular, Example 4 (ultrasound-assisted extraction) achieved the best results, demonstrating that ultrasound-assisted extraction can better preserve the active ingredients and activity of medicinal materials.

[0129] 4. Synergistic Effect of Replenishing Vital Qi and Activating Blood Circulation and Unblocking Meridians: The therapeutic effects of Comparative Example 1 (lacking Vital Qi-replenishing drugs) and Comparative Example 2 (lacking blood circulation and unblocking meridians drugs) were significantly lower than those of Examples 1-8. This demonstrates that both replenishing vital Qi and activating blood circulation and unblocking meridians are essential for treating stroke sequelae and require synergy to achieve optimal results. This also confirms the theory of stroke pathogenesis described in "Yi Lin Gaicuo" (Medical Correction of Errors) as "vital Qi deficiency, Qi deficiency, and blood stasis."

[0130] 5. Influence of Preparation Process: The therapeutic effects of Comparative Example 3 (traditional extraction process) and Comparative Example 5 (different extraction solvents) were significantly lower than those of Examples 1-8, indicating that the step-by-step extraction process and solvent selection of the present invention have an important impact on the quality and efficacy of the preparation.

[0131] 6. Effect of honey: The therapeutic effect of Comparative Example 4 (no honey added) was slightly lower than that of Example 1, which indicates that the addition of honey helps to improve the stability and bioavailability of the preparation, thereby improving the therapeutic effect.

[0132] Comprehensive analysis shows that the Longteng Restoring Ointment, through its dual mechanism of action of "replenishing vital energy and promoting blood circulation and unblocking meridians," exhibits significant therapeutic efficacy for post-stroke sequelae. Astragalus polysaccharide activates the PI3K / Akt signaling pathway, improving mitochondrial function and ATP production, thereby restoring neuronal membrane potential. Hirudin and safflower yellow inhibit platelet aggregation, dissolve thrombi, and improve cerebral microcirculation. Ferulic acid and ligustilide in angelica alleviate oxidative stress and protect neurons. These components work synergistically at the molecular level, exerting their therapeutic effects on post-stroke sequelae.

[0133] Furthermore, the present invention's step-by-step extraction process and application of modern extraction technologies fully extract and retain the active ingredients of each medicinal material, ensuring the quality and efficacy of the preparation. In particular, the application of ultrasound-assisted extraction and supercritical CO₂ extraction significantly increases the extraction rate of macromolecular compounds such as hirudin and earthworm protein, as well as volatile oil components, further enhancing the preparation's efficacy.

[0134] Comparing the above examples with the evaluation experiments, Example 4 (Ultrasound-Assisted Extraction of Longteng Restoring Cream) demonstrated the best results across all indicators and was therefore determined to be the optimal embodiment of the present invention. Its unique feature is the introduction of ultrasound-assisted extraction technology into the preparation process, which significantly increases the extraction rate of macromolecular compounds such as hirudin and earthworm protein, thereby enhancing the preparation's blood circulation and neuroprotective effects.

[0135] In actual application, the appropriate dosage and course of treatment can be selected according to the patient's specific condition and physical characteristics. Generally, 10-15 ml each time, 2-3 times a day, with warm water, is taken. One month is a course of treatment, and generally 2-3 courses are taken continuously.

[0136] The Longteng Restoring Ointment of the present invention is suitable for treating the sequelae of stroke, especially the sequelae of stroke caused by deficiency of vital energy and qi deficiency and blood stasis, such as facial paralysis, hemiplegia, drooling, dry stool, frequent urination, incontinence, as well as symptoms of minor stroke caused by deficiency of vital energy, such as dizziness, vomiting, and numbness and discomfort in the face.

[0137] The following precautions should be taken when using this product: 1. Pregnant women are prohibited from using this product. 2. Use with caution in those with Yin deficiency and hyperactivity of fire. 3. Use with caution in those with spleen and stomach deficiency and coldness. 4. Use with caution in those with hyperactivity of liver yang. 5. Avoid spicy and irritating foods while taking this product.

[0138] The foregoing is merely an embodiment of the present invention and is not intended to limit the present invention. It will be apparent to those skilled in the art that various modifications and variations of the present invention are possible. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention are intended to be included within the scope of the claims of the present invention.

Claims

1. Longteng Recovery Cream, characterized by: In parts by weight, the Longteng Restoring Ointment includes the following components in parts by weight: 55-65 parts of Astragalus, 25-35 parts of Millettia reticulata, 25-35 parts of Sargentodoxa chinensis, 5-8 parts of peach kernel, 5-8 parts of safflower, 12-18 parts of leech, 12-18 parts of angelica, 20-25 parts of white peony root, 12-18 parts of earthworm, 12-18 parts of Alpinia oxyphylla, 25-35 parts of Rehmannia glutinosa, 12-18 parts of Chuanxiong, 12-18 parts of red peony root, 25-35 parts of Cuscuta chinensis, 12-18 parts of mistletoe, and 5-8 parts of costus root.

2. The preparation method of Longteng Recovery Cream according to claim 1, characterized in that: The following steps are involved: A. Raw Material Pretreatment: Remove impurities from each component medicinal material, wash and dry them; cut Astragalus, Angelica, White Peony Root, Red Peony Root, and Chuanxiong into 1-3 mm slices; cut Millettia Spatholobi and Sargentodoxae Caulis into 3-5 mm slices; crush Leech and pass through a 40-mesh sieve; crush the remaining medicinal materials and pass through a 20-mesh sieve; B. Extraction: Group the processed herbs for extraction; C. Combine the extracts: Combine the extracts from each group; D. Concentration: Concentrate the combined extracts under reduced pressure; E. Preparation: Add honey to the concentrate and mix well to obtain the Longteng recovery cream.

3. The method according to claim 2, characterized in that The extraction in step B comprises: B1. Water Extraction: Astragalus, Millettia reticulata, Sargentodoxae, Angelica sinensis, White Peony Root, Earthworm, Rehmannia glutinosa, Chuanxiong, Paeonia lactiflora, Cuscuta chinensis, and Morus alba were added to an extraction tank and soaked in 10 times the amount of water for 30 minutes. The mixture was heated to 90-95°C and refluxed for 2 hours. The mixture was filtered while hot. The residue was then extracted with 8 times the amount of water for 1.5 hours; then 6 times the amount of water for 1 hour. The filtrates were combined. B2. Alcohol extraction: Peach kernel, safflower, Alpinia oxyphylla, and costus root were added to an extraction tank and soaked in 8 times the volume of 70% ethanol for 4 hours. The mixture was heated to 60-65°C and refluxed for 1.5 hours. The mixture was filtered while hot. The residue was then extracted with 6 times the volume of 70% ethanol for 1 hour. The two filtrates were combined. B3. Leech extraction: Add leech powder to an extraction tank, add 12 times the amount of purified water, and extract at a constant temperature of 45-50°C for 4 hours while stirring. Filter and collect the filtrate.

4. The method according to claim 3, characterized in that The pH value of the water extraction in step B1 is 6.5-7.0; the pH value of the leech extraction in step B3 is 6.0-7.

0.

5. The method according to claim 2, characterized in that The step B may also optionally include: Ultrasonic-assisted extraction: Add the crushed earthworms and leeches into an ultrasonic extraction tank, add purified water, the solid-liquid ratio is 1:12, the ultrasonic power is 500W, the frequency is 40kHz, the temperature is 45℃, the time is 30 minutes, and the extract is filtered for later use.

6. The method according to claim 2, characterized in that The step B may also optionally include: Supercritical CO2 extraction: Put the powders of costus root, ligusticum chuanxiong and angelica sinensis into the extractor, extract for 2 hours at a pressure of 25 MPa, a temperature of 45°C and a synergist of 5% ethanol, and collect the extract.

7. The method according to claim 2, characterized in that The conditions for reduced pressure concentration in step D are: temperature 60-65° C., vacuum degree -0.08 MPa, and the concentration end point is a relative density of 1.25-1.30 (60° C.).

8. The method according to claim 2, characterized in that The amount of honey added in step E is 30% of the weight of the concentrate, the mixing temperature is 50-55° C., the homogenization speed is 60-80 rpm, and the homogenization time is 30 minutes.

9. The method according to claim 2, characterized in that It also includes filling and sterilization steps: filling the ointment into a sterilized glass bottle according to the specified amount of 200ml±5ml, tightening the bottle cap, heat sterilizing at 100℃ for 30 minutes, and labeling after cooling.

10. Use of the Longteng Restoring Ointment according to claim 1 in the preparation of a medicament for treating post-stroke symptoms, wherein the post-stroke symptoms include facial paralysis, hemiplegia, drooling, dry stool, frequent urination, incontinence, and dizziness, vomiting, and facial numbness and discomfort caused by a minor stroke due to insufficient vital energy.