Indoor algae-be symbiotic artificial culture system and method

Abstract

The application discloses an indoor algae-be symbiotic artificial culture system and method, and belongs to the field of artificial culture of algae-be symbiotic system. The system comprises a culture box with a box cover, an attachment substrate device and a water environment control system. A partition plate is vertically arranged in the culture box to form a water flow channel between the partition plate and the inner wall of the culture box, and two functional areas, i.e. a first area and a second area, are formed on the two sides of the partition plate. The attachment substrate device is arranged in the second area. The water environment control system comprises a flow rate regulating unit, a light unit, a heating unit, a dissolved oxygen unit and a control unit. The flow rate regulating unit, the light unit, the heating unit and the dissolved oxygen unit are arranged in the first area and are controlled by the control unit. The method comprises the following steps: adding shellfish to the attachment substrate device; adding algae to the second area; and regulating the water flow rate, the light intensity, the water temperature and the water dissolved oxygen by the water environment control system. The application is applied to the indoor artificial breeding of freshwater zebra mussels and can improve the survival rate of the artificial breeding of the freshwater zebra mussels.

Description

TECHNICAL FIELD

[0001] The present inventors are involved in the technical field of artificial culture of freshwater zebra mussels, and particularly in an indoor algal-be symbiotic artificial culture system and method. BACKGROUND

[0002] The freshwater zebra mussel, scientifically known as Dreissena polymorpha, belongs to the family Dreissenidae in the order Mytilida and is one of the common fouling organisms in inter-basin water diversion projects. On the one hand, the massive attachment and reproduction of the freshwater zebra mussel on parts such as water diversion pipes can cause blockage of the water diversion pipes, thereby reducing the water diversion capacity, and the attachment of the freshwater zebra mussel on the water diversion pipes can also cause damage to the surface layer of the pipes and accelerate the corrosion of the pipes, seriously threatening the structural safety of water conservancy projects. On the other hand, as an invasive species, the freshwater zebra mussel can spread to new water bodies through inter-basin water diversion projects, thereby disrupting the balance of the ecological system. Obviously, it is necessary to study the freshwater zebra mussel.

[0003] In related technologies, research on the freshwater zebra mussel is mostly carried out in field observation experiments in different regions or in indoor simulation experiments of the habitat of the freshwater zebra mussel. Among them, field experiments have high research costs and are difficult to carry out, and indoor experiments also have high requirements for the control of the indoor growth and reproduction environment of the freshwater zebra mussel, and the freshwater zebra mussel is difficult to survive for a long time in the indoor environment.

[0004] Therefore, how to make the freshwater zebra mussel survive for a long time in the indoor environment to lay a foundation for the basic ecology and environmental tolerance research of the freshwater zebra mussel is also a top priority of the present invention. SUMMARY

[0005] To solve or partially solve the problems in the related art, the present invention provides an indoor algal-be symbiotic artificial culture system and method, which can effectively improve the survival rate of artificial breeding of the freshwater zebra mussel and realize indoor artificial reproduction of the freshwater zebra mussel.

[0006] The present invention provides an indoor algal-be symbiotic artificial culture system, which comprises a culture box with a box cover, an attachment substrate device, and a water environment control system. A partition plate is vertically arranged in the culture box to form a water flow channel between the partition plate and the inner wall of the culture box, and two functional areas, a first area and a second area, are formed on both sides of the partition plate.

[0007] The attachment substrate device is arranged in the second area. The water environment control system comprises a flow rate control unit, a lighting unit, a heating unit, a dissolved oxygen unit, and a control unit. The flow rate control unit, the lighting unit, the heating unit, and the dissolved oxygen unit are arranged in the first area and are controlled by the control unit.

[0008] The flow rate regulating unit comprises: a water-stopping partition plate erected in the first area, one end of the water-stopping partition plate being connected to the partition plate and the other end being connected to the inner wall of the incubator; and a submersible pump located on the water inlet side of the water-stopping partition plate, the submersible pump being connected to a hole provided on the water-stopping partition plate through a water outlet pipe or the water outlet pipe penetrating through the hole; when the submersible pump is started, the water on the water inlet side of the water-stopping partition plate flows out from the water outlet side of the water-stopping partition plate through the water outlet pipe, so that the water contained in the incubator circulates along the water flow channel.

[0009] The light unit, the heating unit and the dissolved oxygen unit are arranged on the water outlet side of the water-stopping partition plate.

[0010] In an optional embodiment, the cross section of the incubator is in the shape of a racetrack or an ellipse.

[0011] In an optional embodiment, the partition plate is arranged along the long axis direction of the cross section of the incubator.

[0012] In an optional embodiment, a plurality of holes are provided on the water-stopping partition plate, the holes are clamping device holes, and the clamping device holes are provided with on-off valves, the on-off valves being used to control the conduction or isolation of the clamping device holes; the water outlet pipe penetrates through one of the clamping device holes.

[0013] In an optional embodiment, the light unit comprises a light source suspended above the second area.

[0014] In an optional embodiment, the attachment substrate device comprises a frame and at least one type of attachment material: the frame is arranged at the bottom of the second area; and the attachment material is laid in the frame.

[0015] In an optional embodiment, a flow stabilizing grid plate and a trash blocking grid plate are erected in the incubator, the flow stabilizing grid plate and the trash blocking grid plate being arranged on opposite sides of the partition plate; the trash blocking grid plate is located on the same side of the water-stopping partition plate as the partition plate, one end of the trash blocking grid plate being connected to the first end of the partition plate and the other end being connected to the inner wall of the incubator; the submersible pump is located between the trash blocking grid plate and the water-stopping partition plate; the flow stabilizing grid plate and the attachment substrate device are located on the same side of the partition plate, one end of the flow stabilizing grid plate being connected to the second end of the partition plate and the other end being connected to the inner wall of the incubator.

[0016] In an optional embodiment, a trash intercepting groove is arranged at the bottom of the incubator, the trash intercepting groove being arranged on the extension line of the partition plate and being close to the water inlet side of the water-stopping partition plate; and a filter opening is arranged at the bottom of the trash intercepting groove.

[0017] The second aspect of the present application provides an indoor algal-bean symbiotic artificial culture method, which is performed by using the indoor algal-bean symbiotic artificial culture system described above, and comprises the following steps:

[0018] Injecting water body into the incubator;

[0019] Adding the shellfish to the attached substrate device;

[0020] Adding algae to the second area on the water outlet side of the water stop baffle;

[0021] Using the flow rate control unit, the light unit, the heating unit, and the dissolved oxygen unit to control the water flow rate, the light intensity, the water temperature, and the water dissolved oxygen to reach the preset value;

[0022] Covering the box cover.

[0023] In an optional embodiment, the shellfish is added at a density of 900 / m 2 -1100 / m 2 ; and the algae cultivation density in the incubator is controlled to be 3×10 5 -5×10 5 cells / L.

[0024] The technical solution provided by the present application can include the following beneficial effects:

[0025] The separation plate is used to form a water flow channel in the incubator, and the flow rate control unit is used to realize the circulation of the water body in the incubator, forming an internal circulation flow mode. In the algae-shellfish cultivation process, the internal circulation flow mode is adopted, without the need to introduce external water body, which can reduce the exchange and contact between the experimental water body and the outside, and can more finely simulate the natural habitat environment of algae and shellfish in combination with the water environment control system. The corner of the water flow channel is designed as an arc, which is more in line with the characteristics of hydraulics. The two functional areas are at a certain distance in the water flow direction, which can improve the stability of the water environment in the second area where the attached substrate device is located.

[0026] It can be seen that the present application has a simple structure and can improve the ecological balance of freshwater shellfish and algae and promote the survival rate of artificial breeding of freshwater shellfish when applied to indoor artificial breeding of freshwater shellfish. It has been proved that the algae-shellfish symbiotic cultivation device can realize indoor cultivation and breeding of freshwater shellfish for one year, and the survival rate of the shellfish is more than 80%. BRIEF DESCRIPTION OF DRAWINGS

[0027] The above and other objects, features and advantages of the present application will become more apparent from the following detailed description when taken in conjunction with the accompanying drawings, in which like reference characters refer to like parts throughout the figures, and wherein:

[0028] Figure 1 is a perspective view of the algae-shellfish symbiotic cultivation device (the box cover is in an open state) according to an embodiment of the present application;

[0029] Figure 2 isFigure 1 is a top view of the algae-behavior symbiotic culture device (the box cover is in the closed state) according to an embodiment of the present application;

[0030] Figure 3 is a top view of the algae-behavior symbiotic culture device (the box cover is in the closed state) according to an embodiment of the present application;

[0031] Figure 4 is Figure 1 is a schematic diagram of the water stop plate, wherein, Fig. (a) is a front view, and Fig. (b) is a side view;

[0032] Figure 5 is a structural block diagram of the water environment control system;

[0033] Figure 6 is a schematic diagram of the light unit;

[0034] Figure 7 is a schematic diagram of the attachment substrate device;

[0035] Figure 8 is a schematic diagram of the steady flow grid plate;

[0036] Figure 9 is a schematic diagram of the trash grid plate and a partial enlarged view.

[0037] In the figure: box 11, box cover 12, water tank 13, first area 14, second area 15, water flow channel 16, partition plate 21, water stop plate 31, detent hole 32, water inlet side 33, water outlet side 34, attachment substrate device 4, frame 41, attachment material 42, rock 43, pebble 44, concrete block 45, water environment control system 5, control unit 51, on-off valve 52, light unit 53, support 531, LED light source 532, screw rod 533, screw buckle 534, cross beam 535, heating unit 54, dissolved oxygen unit 55, submersible pump 61, water outlet pipe 62, steady flow grid plate 71, steady flow grid 72, trash grid plate 81, trash grid 82, trash collection tank 91, filter opening 92. DETAILED DESCRIPTION

[0038] Embodiments of the present application will be described in more detail by referring to the attached drawings. Although embodiments of the present application are shown in the drawings, it should be understood that the present application can be implemented in various forms and should not be limited by the embodiments set forth herein. Rather, these embodiments are provided so that the present application is more thoroughly and completely conveyed to those skilled in the art, and the scope of the present application is fully conveyed to those skilled in the art.

[0039] The terminology used in the present application is for the purpose of describing particular embodiments only and is not intended to be limiting of the present application. As used in the present application and the accompanying claims, the singular form "a", "an" and "the" include plural references unless the context clearly dictates otherwise. It is also to be understood that the term "and / or" as used herein refers to and encompasses any and all possible combinations of one or more of the associated listed items.

[0040] It should be understood that, although the terms "first", "second", "third" and the like can be used herein to describe various information, these information should not be limited to these terms. These terms are only used to distinguish one piece of information from another piece of information. For example, without departing from the scope of the present application, the first information can also be referred to as the second information, and similarly, the second information can also be referred to as the first information. Therefore, the features defined with "first", "second" can explicitly or implicitly include one or more of the features. In the description of the present application, the meaning of "a plurality of" is two or more, unless otherwise specifically limited.

[0041] In the related art, indoor experiments also have high requirements for the control of the indoor growth and reproduction environment of the freshwater mussel, and the freshwater mussel is difficult to survive for a long time indoors, generally surviving for less than a month, with a survival rate of less than 40%, and the larvae cannot effectively survive. How to make the freshwater mussel survive for a long time indoors for growth and reproduction to lay a foundation for the basic ecology and environmental tolerance research of the freshwater mussel is also the most important of the present application.

[0042] In view of the above problems, the present application provides a single-box type algae-bean symbiotic culture device, which can improve the ecological balance of freshwater mussels and algae, promote the survival rate of artificial breeding of freshwater mussels, and realize indoor artificial breeding of freshwater mussels.

[0043] The technical solutions of the embodiments of the present application are described in detail below with reference to the drawings.

[0044] Referring to Figures 1-3 The present application provides a single-box type algae-bean symbiotic culture device, which comprises a culture box, a water environment control system and an attachment substrate device 4.

[0045] In some embodiments, the culture box comprises a box body 11 and a box cover 12.

[0046] The water tank 13 is formed in the box 11 and is used to contain water for experiments. The water tank 13 is formed by the side plates of the box 11 and the bottom plate of the box 11. The cross section of the water tank 13 can be in the shape of a racetrack or an ellipse. In one embodiment, the cross section of the water tank 13 is formed by two semicircles with equal radii connected to two short sides of a rectangle. In this embodiment, the approximate dimensions of the water tank 13 are 140 cm in length, 40 cm in width and 60 cm in height. The length of the rectangular part is 100 cm and the radius of the semicircular part is 20 cm.

[0047] In some embodiments, the partition plate 21 is arranged in the box 11 and forms a water flow channel 16 between the partition plate 21 and the inner wall of the box 11. The box 11 is divided into two functional areas, i.e. the first area 14 and the second area 15, by the partition plate 21. The first area 14 and the second area 15 are both on the water flow channel 16. Further, the partition plate 21 is arranged along the long axis of the box 11 and has the same length as the long side of the rectangular part.

[0048] In this embodiment, the approximate dimensions of the partition plate 21 are 100 cm in length and 60 cm in height. The partition plate 21 is arranged in the water tank 13 and forms a water flow channel 16 in the single-chamber water tank, thereby transforming the single-chamber water tank into a circulating water tank.

[0049] The functional area where the attachment substrate device 4 is arranged is the second area 15, and the functional area where the flow rate control unit is arranged is the first area 14. The first area 14 and the second area 15 on both sides of the partition plate 21 are both straight channels with approximate dimensions of 100 cm in length, 20 cm in width and 60 cm in height.

[0050] Further, the flow rate control unit includes a water-stopping partition plate 31 and a submersible pump 61, which are used to control the circulation of water in the box 11 and to regulate the flow rate of water. In some embodiments, the water-stopping partition plate 31 is arranged in the box 11, specifically in the first area 14. One end of the water-stopping partition plate 31 is connected to the partition plate 21 perpendicularly, and the other end is connected to the inner wall of the box 11 perpendicularly. The approximate dimensions of the water-stopping partition plate 31 are 20 cm in length and 60 cm in height. The water-stopping partition plate 31 is arranged in the straight channel of the first area 14 to block the water flow.

[0051] Please refer to Figure 4Furthermore, multiple positioning holes 32 are provided on the water-stop baffle 31, and the positioning holes 32 have a centrally located tube structure. In some embodiments, a 3×3 array of positioning holes is provided on the water-stop baffle 31, with a hole diameter of 25mm and a tube length of 100mm. The submersible pump 61 is connected to a positioning hole 32 through a water outlet pipe 62. Specifically, the water outlet pipe 62 passes through the positioning hole 32 and is fixed to the water-stop baffle 31. When the submersible pump 61 is started, the water on the inlet side 33 of the water-stop baffle 31 flows out from the outlet side 34 through the water outlet pipe 62, thereby circulating the water in the tank 11. In addition, the water flow rate can be controlled by adjusting the power of the submersible pump 61. The submersible pump 61 can be a DC submersible pump with a rated voltage of 0-72V, a rated power of 0-500W, a rated flow rate of 0-8m3 / h, and an outlet diameter of 25mm.

[0052] by Figures 1-2 For example, when the submersible pump 61 is started, the water on the inlet side 33 of the water-stop baffle 31 passes through the water-stop baffle 31, sequentially through the first region 14 and the second region 15, and then returns to the inlet side 33 of the water-stop baffle 31, thus forming an internal circulation flow in the clockwise direction within the water tank 13. Using an internal circulation mode during algae and shellfish cultivation eliminates the need to introduce external water, reducing the exchange and contact between the experimental water and the outside environment, and better simulating the algae and shellfish habitat environment. Therefore, the water in the water tank 13 can flow, which can be called an internal circulation mode. In contrast, an external circulation mode can be understood as the water tank 13 having an inlet and an outlet. The outlet of the water tank 13 is connected to the inlet of the water tank 13 through an external water collection tank to achieve water circulation.

[0053] In this embodiment, the first region 14 and the second region 15 are a certain distance apart on the water flow channel 16. Therefore, after controlling the water environment, the stability of the water environment in the second region 15 where the substrate attachment device 4 is located can be improved.

[0054] The purpose of having multiple locking holes 32 through the above-mentioned water-stop baffle 31 is to adjust the height and direction of the water outlet. These locking holes 32 are distributed at different heights and positions on the water-stop baffle 31. By fixing the water outlet pipe 62 into different locking holes 32, the height and direction of the water outlet can be adjusted. This, combined with adjusting the operating power of the submersible pump 61, allows for the control of the water flow rate. It should be noted that when the submersible pump 61 is started, all locking holes 32 except those with the water outlet pipe 62 fixed in them remain closed. Furthermore, each locking hole 32 is equipped with a switch valve 52, which controls the opening or closing of the locking hole 32. Figure 4 The switch valve 52 in the diagram is for illustrative purposes only, indicating that the switch valve is normally in the closed state, at which time the card slot channel 32 is in the isolated state.

[0055] The conduction of different check position hole 32 can correspond to different flow rates, which is based on the different water outlet height and direction of different check position hole 32, so as to cooperate with the submersible pump 61 to regulate the flow rate of water.

[0056] In some embodiments, the water environment control system 5 further comprises one or more of the illumination unit 53, the heating unit 54 and the dissolved oxygen unit 55, which are connected with and controlled by the control unit 51. The illumination unit 53, the heating unit 54 and the dissolved oxygen unit 55 are also arranged in the second area 15, but are arranged on the opposite side of the water stop partition plate 31 from the submersible pump 61, that is, the illumination unit 53, the heating unit 54 and the dissolved oxygen unit 55 are arranged on the water outlet side 34 of the water stop partition plate 31, and the submersible pump 61 is arranged on the water inlet side 33 of the water stop partition plate 31. The control unit 51 can be an integrated controller or a combination of multiple split controllers. In this embodiment, the control unit 51 can include a flow rate controller connected with the submersible pump 61.

[0057] As shown in Figure 6 In at least one embodiment, the illumination unit 53 includes a bracket 531 and an LED light source 532 arranged on the bracket 531, the LED light source 532 being connected with and controlled by the control unit 51, and the LED light source 532 being configured to be adjustable in height. In one embodiment, the control unit 51 can include an LED illumination controller electrically connected with the LED light source 532, and the LED light source 532 can be an LED lamp tube with a length of 60 cm, a color temperature of 6500 K and a cold white light source. The LED lamp tube is fixed on the bracket 531, and the bracket 531 can be installed with 0-5 adjustable LED lamp tubes, each LED lamp tube being distributed along the water flow direction, and the bracket 531 being suspended above the second area 15. The bracket 531 is an I-shaped bracket with a length of 50 cm and a width of 15 cm, and a screw rod 533 with a length of 26 cm is arranged directly above the center of the I-shaped bracket 531. The screw rod 533 is connected to the center of a crossbeam 535 above the water flow channel 16 through a screw buckle 534, and the crossbeam 535 is arranged on the partition plate 21 and the tank 11. The height of the bracket 531 on the crossbeam 535 is changed by the upper and lower screw buckles 534, so as to adjust the height of the bracket 531 from the bottom of the water tank 13. The combination of the screw rod 533, the screw buckle 534 and the crossbeam 535 is called a height adjuster of the bracket 531, which can adjust the height in a range of 0-25 cm, and the working time of the LED lamp tube is adjusted by the LED illumination controller to regulate the illumination intensity and time.

[0058] In at least one embodiment, the heating unit 54 includes a heating element located below the illumination unit 53 and mounted on the bottom of the housing 11. The heating element is connected to the control unit 51. In one embodiment, the control unit 51 may include a temperature controller. The heating element may be a digital display heating rod made of rectangular ceramic heating material with a rated power of 100W, a temperature control range of 18℃-35℃, and approximate dimensions of 15cm in length, 4cm in width, and 3cm in height. The digital display heating rod is located below the LED light source 532, and its back suction cup is fixed to the bottom of the water tank 13. It works in conjunction with the temperature controller to adjust its operating status and regulate the water temperature.

[0059] In at least one embodiment, the dissolved oxygen unit 55 includes an aeration stone, an oxygen pipe, and an oxygenation pump. The aeration stone is located below the LED light source 532 and installed at the bottom of the housing 11. The oxygenation pump is connected to the aeration stone via the oxygen pipe and is electrically connected to the control unit 51. In one embodiment, the control unit 51 may include a dissolved oxygen controller. The aeration stone can be a high-density diamond fine sand disc, with a diameter of 4cm-6cm and a thickness of 1.5cm. The rated operating voltage of the oxygenation pump is AC220V±10%, the rated power is 12W, and the air output is 0-20L / min. The aeration stone is located below the LED light source 532, and its back suction cup is fixed to the bottom of the water tank 13. The aeration stone is connected to the oxygenation pump via the oxygen pipe, and its working state is adjusted in conjunction with the dissolved oxygen controller to regulate the dissolved oxygen in the water.

[0060] In at least one embodiment, the water environment control system 5 further includes a display unit connected to the control unit 51, used to display at least one of flow rate information, temperature information, illumination information, and dissolved oxygen information. The display includes a digital display control screen, which is a touch-sensitive OLED screen. The submersible pump 61, heating element, LED lamp, and oxygenation pump are independently controlled by various controllers in the control unit 51 to display and monitor relevant information. For example, through the control unit 51, the submersible pump 61 can be set to control the water flow rate at 0.1 m / s-0.2 m / s, the digital display heating element can be set to control the water temperature at 22℃-24℃, and the oxygenation pump can be set to control the dissolved oxygen in the water at 6 mg / L-9 mg / L, etc.

[0061] like Figure 7 As shown, the attachment substrate device 4 further includes a frame 41 and at least one type of attachment material 42, which is laid within the frame 41. The at least one type of attachment material 42 includes at least one of rock 43, pebble 44, and concrete block 45. The attachment substrate device 4 includes at least two types of attachment materials 42, which are laid at different locations within the frame 41.

[0062] The second area 15 is an experimental area. The frame 41 is rectangular, with a length of 80 cm and a width of 20 cm. The frame 41 is embedded in the bottom of the second area 15. The frame 41 is uniformly paved with substrate materials for inducing the attachment of freshwater shellfish. In this embodiment, the attachment materials 42 include rocks 43, pebbles 44, and concrete blocks 45, which are combined in a ratio of 1:1:1. The particle size of each type of material is 5-12 cm, and the porosity is 10-20%. When culturing freshwater shellfish and algae in the tank 13, the box cover 12 at least fully covers the second area 15. The box cover 12 is a light-shielding and heat-insulating cover plate that can be opened and closed, such as a black opaque foam KT plate. The size of the box cover 12 is 95 cm in length and 40 cm in width. Obviously, the box cover 12 can also cover the first area 14.

[0063] As a preferred embodiment of the present application, the stable flow grid plate 71 and the trash grid plate 81 are arranged at both ends of the partition plate 21. The two ends of the partition plate 21 are referred to as the first end and the second end, respectively. The stable flow grid plate 71 is connected to the second end of the partition plate 21, and the trash grid plate 81 is connected to the first end of the partition plate 21. The stable flow grid plate 71 is located in the second area 15, and the trash grid plate 81 is located in the first area 14.

[0064] As shown in Figure 8 In at least one embodiment, one end of the stable flow grid plate 71 is connected perpendicularly to the second end of the partition plate 21, and the other end is connected to the inner wall of the box body 11. The stable flow grid plate 71 is on the same side of the partition plate 21 as the attachment substrate device 4. The size of the stable flow grid plate 71 is 60 cm in height and 40 cm in width. The stable flow grid plate 71 is provided with an array of rectangular stable flow grids 72. The size of the rectangular stable flow grids 72 is 1 cm x 3 cm, and the long edges are vertically distributed. The distance between the left and right adjacent rectangular stable flow grids 72 is 1 cm, and the distance between the upper and lower adjacent rectangular stable flow grids 72 is 1 cm. The stable flow grid plate 71 is perpendicularly fixed to the partition plate 21 and the inner wall of the box body 11 on both sides through clamping grooves, for stabilizing the water flow rate.

[0065] As shown in Figure 9 In at least one embodiment, one end of the trash grid plate 81 is connected perpendicularly to the first end of the partition plate 21, and the other end is connected to the inner wall of the box body 11. The trash grid plate 81 is on the same side of the partition plate 21 as the water-stopping partition plate 31. In this embodiment, the water-stopping partition plate 31 is located near the first end of the partition plate 21, and a cavity for accommodating the submersible pump 61 is formed between the water-stopping partition plate 31 and the trash grid plate 81. The trash grid plate 81 is used to intercept larger floating and suspended matter in the water body under the premise of ensuring normal water permeability, so as to avoid affecting the normal operation of the submersible pump 61.

[0066] The rectangular trash screen 82 is smaller than the rectangular flow stabilizing screen 72. The size of the trash screen plate 81 is about 60 cm in height and 40 cm in width, and the size of the rectangular trash screen 82 is 0.8 cm by 2 cm, with the long side horizontally distributed. The two ends of the trash screen plate 81 are fixed perpendicularly to the inner wall of the tank 11 and the partition plate 21 through clamping grooves. The trash screen plate 81 is used to intercept larger floating and suspended substances in the water body under the premise of ensuring normal water permeability, so as to avoid affecting the normal operation of the submersible pump.

[0067] As a preferred embodiment of the present application, the tank 11 is further provided with a sewage interception groove 91. In at least one embodiment, the sewage interception groove 91 is connected to the first end of the partition plate 21 at the bottom of the water flow channel 16 and is located on the extension line of the partition plate 21. In this embodiment, the bottom of the sewage interception groove 91 is provided with a filter opening 92, and the cross section of the sewage interception groove 91 is a trapezoid with the upper base longer than the lower base. The sewage interception groove 91 is arranged upstream of the trash screen plate 81 and downstream of the attached substrate device 4, and is located at the bottom of the water tank 13. The lower base of the sewage interception groove 91 is provided with a filter opening 92, which is generally in a closed state and is opened to discharge larger suspended and precipitated substances in the water body when needed. The sewage interception groove 91 is located on the extension line of the partition plate 21, and the size of the sewage interception groove 91 is about 20 cm in length, with a trapezoidal cross section, 2 cm in width of the upper base, 1.5 cm in width of the lower base, and 1 cm in depth. The filter opening 92 is located at the center of the bottom of the sewage interception groove 91 and is a circular hole with a diameter of 1.5 cm. The filter opening 92 can be connected to a drain pipe for intercepting and discharging larger suspended and precipitated substances in the water flow.

[0068] It can be seen that the single-box type algae-behavior symbiotic culture device provided by the embodiment of the present application has a simple structure, adopts a semicircular design at the corner of the water flow channel 16, is more in line with the characteristics of hydraulics, and can reduce the exchange and contact between the experimental water body and the external environment by using an internal circulation mode. In combination with the water environment control system 5 that can regulate and control the water environment, the natural habitat environment of algae-behavior can be simulated more finely, the survival rate of indoor freshwater mussel artificial breeding can be significantly improved, and long-term stable and effective protection can be ensured. It has been proved by practice that the algae-behavior symbiotic culture device of the present application can realize indoor cultivation and reproduction of freshwater mussels in one year, and the survival rate of the mussels is more than 80%.

[0069] More specifically, after using the present application for a period of time in freshwater mussel breeding, it can be observed that the larvae grow and reproduce, so that the present application can realize the reproduction of freshwater mussel larvae and the breeding of freshwater mussels in the water tank 13, and realize the artificial reproduction of freshwater mussels indoors.

[0070] The embodiment of the present application also provides an artificial culture method using the indoor algae-be symbiotic artificial culture system.

[0071] S100: injecting water into the water tank 13 of the box 11;

[0072] In step S100, water for experiment is added into the water tank 13, and the water depth is controlled to be (20±1) cm.

[0073] S200: adding the shellfish on the attachment substrate device 4;

[0074] In step S200, live freshwater mussel spat collected from the wild is added on the attachment substrate device 4, wherein the culture and release density is controlled to be 900 / m 2 -1100 / m 2 The shellfish culture and release density refers to the number of shellfish per unit area of the attachment substrate device.

[0075] S300: adding algae into the second area;

[0076] In step S300, the artificial culture green algae liquid is added on the water outlet side of the water stop baffle 31 and below the LED lamp tube, and the algae culture density in the water tank 13 is controlled to be 3×10 5 -5×10 5 cell / L. The algae culture density refers to the number of algae cells per unit water.

[0077] S400: regulating the water flow rate, light intensity, water temperature and water dissolved oxygen by using the flow rate regulating unit, light unit, heating unit 54 and dissolved oxygen unit 55;

[0078] In step S400, the water is circulated at a flow rate of 0.1-0.2 m / s by using the flow rate regulating unit; the light intensity is controlled to be 8500 Lux-8700 Lux, and the working and intermittent time of the LED lamp tube is set to be 12 h:12 h; the water temperature is controlled to be (23±1) ℃ by using the heating unit 54; and the water dissolved oxygen is controlled to be 6-9 mg / L by using the dissolved oxygen unit 55.

[0079] S500: after the above process is completed, the box cover 12 is closed to achieve the effect of heat preservation and light shielding, and the freshwater mussel spat is kept in a dark light environment.

[0080] After the freshwater mussel spat stably attaches and grows for a certain period of time, the box cover 12 can be opened, and the growth condition of the freshwater mussel spat in the water tank 13 can be observed, or part of the live freshwater mussel spat can be taken out for research and experiment.

[0081] Particularly, the algal-be co-culture device can be used to grow the freshwater zebra mussels into adult mussels within one year, during which the freshwater zebra mussels reproduce larvae and the adult mussels are bred. Therefore, the optimal hydrological rhythm required for the reproduction of the freshwater zebra mussels can be determined in advance, the water temperature and flow rate in the water tank are adjusted to simulate the optimal hydrological rhythm for the reproduction of the freshwater zebra mussels, and the algae food in the water tank is controlled to be sufficient. After the freshwater zebra mussels are stably attached and grow for a certain period of time, the normal reproduction of larvae and breeding of adult mussels of the freshwater zebra mussels can be observed.

[0082] The above has described various embodiments of the present application, and the above description is exemplary and is not exhaustive, and is not limited to the disclosed embodiments. Many modifications and changes are obvious to those skilled in the art without departing from the scope and spirit of the described embodiments. The selection of the terms used herein is intended to best explain the principles of the embodiments, practical application, or improvement of the technology in the market, or to enable other ordinary skilled in the art to understand the embodiments disclosed herein.

Claims

1. A method for indoor artificial cultivation of algae and shellfish symbiosis, characterized in that: Artificial cultivation was carried out using an indoor algae-shellfish symbiotic artificial cultivation system, which included a cultivation box with a lid, an attachment substrate device, and a water environment control system. The incubator is equipped with a partition plate to form a water flow channel between the partition plate and the inner wall of the incubator, and two functional areas are formed on both sides of the partition plate: a first area and a second area. The attachment substrate device is deployed in the second area; The water environment control system includes a flow rate regulation unit, a lighting unit, a heating unit, a dissolved oxygen unit, and a control unit. The flow rate regulation unit, the lighting unit, the heating unit, and the dissolved oxygen unit are all located in the first area and are all controlled by the control unit. The flow rate control unit includes: A water-stop partition is erected in the first area, with one end connected to the partition plate and the other end connected to the inner wall of the incubator; A submersible pump is located on the inlet side of the water-stop baffle. The submersible pump is connected to a channel provided on the water-stop baffle through an outlet pipe, or the outlet pipe passes through the channel. When the submersible pump is started, the water on the inlet side of the water-stop baffle flows out from the outlet side of the water-stop baffle through the outlet pipe, so that the water contained in the incubator circulates along the water flow channel. The lighting unit, heating unit, and dissolved oxygen unit are all located on the water outlet side of the water-stopping baffle. The incubator is equipped with a flow-stabilizing grid plate and a contaminant-blocking grid plate, which are respectively located at both ends of the partition plate. The contaminant-blocking grid plate and the water-stop partition plate are located on the same side of the partition plate, with one end of the contaminant-blocking grid plate connected to the first end of the partition plate and the other end connected to the inner wall of the incubator. The submersible pump is located between the contaminant-blocking grid plate and the water-stop partition plate. The flow-stabilizing grid plate and the substrate attachment device are located on the same side of the partition plate, with one end of the flow-stabilizing grid plate connected to the second end of the partition plate and the other end connected to the inner wall of the incubator. Indoor artificial cultivation methods for algae-shellfish symbiosis include: Fill the incubator with water; Shellfish are added to the attachment substrate device at a density of 900 shellfish / m². 2 -1100 pieces / m 2 ; Algae are added to the second area on the water outlet side of the water-stopping baffle, and the algae cultivation density in the culture tank is 3×10⁻⁶. 5 cell / L-5×10 5 cell / L; The water flow rate, light intensity, water temperature, and dissolved oxygen are controlled to reach preset values ​​using a flow rate control unit, a light intensity unit, a heating unit, and a dissolved oxygen unit. The preset values ​​for water flow rate, light intensity, water temperature, and dissolved oxygen are 0.1 m / s-0.2 m / s, 8500 Lux-8700 Lux, (23±1) ℃, and 6 mg / L-9 mg / L, respectively. The working and intermittent time of the light intensity unit is set to 12h:12h. Close the lid of the box.

2. The indoor artificial cultivation method for algae-shellfish symbiosis as described in claim 1, characterized in that: The cross-section of the incubator is racetrack-shaped or elliptical.

3. The indoor artificial cultivation method for algae-shellfish symbiosis as described in claim 1, characterized in that: The partition plates are arranged along the long axis of the incubator's cross-section.

4. The indoor artificial cultivation method for algae-shellfish symbiosis as described in claim 1, characterized in that: The water-stop baffle is provided with multiple channels, which are positioning device channels, and each positioning device channel is equipped with a switch valve, which controls the opening or closing of the positioning device channel; the water outlet pipe passes through one of the positioning device channels.

5. The indoor artificial cultivation method for algae-shellfish symbiosis as described in claim 1, characterized in that: The illumination unit includes a light source suspended above the second region.

6. The indoor artificial cultivation method for algae-shellfish symbiosis as described in claim 1, characterized in that: The attachment substrate device includes a frame and at least one type of attachment material: the frame is located at the bottom of the second region; the attachment material is laid in the frame.

7. The indoor artificial cultivation method for algae-shellfish symbiosis as described in claim 1, characterized in that: The bottom of the incubator is provided with a sludge trap, which is located on the extension line of the partition plate and close to the water inlet side of the water-stop partition plate; a filter port is opened at the bottom of the sludge trap.

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