Composition for improving microorganisms on skin surface as well as preparation method and application of composition

Abstract

The invention belongs to the field of cosmetics or cleaning supplies, and particularly relates to a composition for improving skin surface microorganisms and a preparation method and application thereof. The composition provided by the invention comprises active ingredients and a solvent, wherein the active ingredients comprise hydrolyzed ginsenoside, a micrococcus lysate, acetylchitosamine, Asian white birch juice and tremella polysaccharide. Through mutual synergism of the active components, beneficial bacteria are promoted and harmful bacteria are inhibited, so that the balance of microorganisms on the skin surface is improved; the effects of resisting oxidation, resisting inflammation, repairing skin, inhibiting and resisting bacteria, relieving and resisting allergy, promoting generation of hyaluronic acid, stripping waste cutin, promoting skin renewal, preserving moisture, improving fine wrinkles, repairing hair, nourishing hair, removing dandruff and / or controlling oil and the like are achieved.

Description

Technical Field

[0001] The present invention relates to the field of cosmetics or cleaning products, and in particular to a composition for improving skin surface microorganisms, a preparation method and an application thereof. Background Art

[0002] As the largest organ in the human body, the skin has a complex and delicate micro-ecosystem on its surface, which is composed of various microbial communities such as bacteria and fungi, as well as tissues, cells and various secretions on the skin surface.

[0003] Skin microecology: The various normal bacterial communities on the skin collaborate, compete, and balance with each other, controlling the skin's stability and forming the skin's microecological system. The microecology is an integral part of the skin, innate in us. Its balance gives the skin its natural resistance, protection, and repair capabilities.

[0004] A balanced skin microbiome is crucial for skin health. When the microbial community is out of balance, known as "skin dysbiosis," it can lead to skin problems such as inflammation, sensitivity, dryness, and other skin disorders. Therefore, maintaining a balanced skin microbiome is crucial for skin health and overall well-being.

[0005] This microbial ecosystem plays an irreplaceable and important role in protecting the skin barrier, regulating immune function, and participating in skin metabolism. Under normal conditions, the microbial community on the skin's surface coexists harmoniously with the human body, establishing a stable equilibrium and jointly resisting the invasion of harmful external substances through various mechanisms. For example, competition among microorganisms for limited nutritional resources can limit the proliferation of harmful bacteria. Beneficial bacteria such as Staphylococcus epidermidis compete with pathogens such as Staphylococcus aureus for nutrients such as sebum. Many beneficial bacteria can also secrete antimicrobial peptides, organic acids, and other substances with bactericidal or antibacterial effects to further combat harmful microorganisms. At the same time, the metabolic activities of microorganisms can regulate the pH of the skin, maintaining a weakly acidic environment on the skin's surface. This is crucial for maintaining the integrity of the skin barrier and promoting the normal metabolism and renewal of the stratum corneum, effectively inhibiting the growth of alkaline-sensitive bacteria.

[0006] However, a variety of factors may lead to imbalance in the skin microecology. Exogenous factors, such as the invasion and competition of foreign bacteria, will greatly reduce the number of resident bacteria or even eliminate them, thereby causing skin damage; endogenous factors include the imbalance of the normal microbial flora, localization transfer and superinfection, etc. Improper skin cleansing (over-cleaning or insufficient cleaning), the influence of the external environment (such as ultraviolet rays), the improper use of irritating products (such as hormones, fruit acids), and the use of cosmetics containing too many additives, etc., may all cause disorders in the skin microecology. Imbalance in the skin microecology will bring many adverse consequences, such as a decrease or even disappearance of probiotics, leading to an imbalance in the flora; reduced skin resistance, prone to inflammation and infection; the skin becomes extremely sensitive and acne breaks out repeatedly; the skin barrier function is weakened, and symptoms such as dryness, tightness, and itching appear, and it may also lead to water and oil imbalance. In addition, studies have found that the imbalance of the skin microecology is closely related to the occurrence and development of various skin diseases such as allergic dermatitis and psoriasis, which will trigger an inflammatory response.

[0007] To maintain a balanced skin microbiome, some products on the market attempt to regulate the skin's microbiome by adding specific ingredients. For example, some skincare products incorporate lactic acid bacteria extracts in the hope of inhibiting the growth of harmful bacteria and reducing skin inflammation. Others incorporate bifidobacterium extracts in an attempt to suppress harmful bacteria by competing for nutrients and adhesion sites, thereby maintaining a stable skin microbiome.

[0008] However, these existing products still have limitations in regulating the skin's microbiome, and are unable to comprehensively and effectively improve the imbalance of the skin's microbiome. For example, some ingredients may only be effective against specific types of harmful bacteria and have poor inhibitory effects on other harmful microorganisms; or, while regulating the microbiome, they may adversely affect other physiological functions of the skin.

[0009] Therefore, developing a composition that can more comprehensively and effectively improve the microorganisms on the skin surface, regulate the balance of the skin microecology, and has no adverse effects on the skin safety has important practical significance and market demand. Summary of the Invention

[0010] In order to solve the above technical problems, the present invention provides the following technical solutions.

[0011] In a first aspect, the present invention provides a composition.

[0012] A composition comprises active ingredients, wherein the active ingredients include hydrolyzed ginsenosides, micrococcal lysate, acetyl glucosamine, Asian birch sap and tremella polysaccharide.

[0013] Hydrolyzed ginsenosides contain a variety of rare saponins, such as rare saponins Rg3, Rh2, Rg2, Rh1, CK, F1, F2, etc. They are easily absorbed and have antioxidant, anti-inflammatory, type I collagen promotion, and anti-hair loss and growth effects.

[0014] Micrococcus is one of the main groups in the skin flora. They are a type of Gram-positive cocci that are commonly found on the surface of human skin. The effects of Micrococcus on the skin include:

[0015] 1. Participate in the formation of skin barrier: Micrococcus can help form an acidic environment on the surface of the skin, which helps prevent the colonization of harmful bacteria.

[0016] 2. Immunomodulation: They can interact with skin cells, affect the activity of immune cells, and thus regulate the immune response.

[0017] 3. Nutritional competition: Micrococci can compete with potential pathogens for nutrients, reducing their growth on the skin.

[0018] 4. Production of antibacterial substances: Certain micrococci can produce antibacterial substances that help inhibit the growth of other harmful bacteria.

[0019] 5. Skin health: The balance of micrococci is essential for maintaining skin health. They can prevent skin from drying out and maintain the skin's moisture balance.

[0020] Micrococcal outer membrane lipopolysaccharide (LPS) can mimic some of the activities of intact bacteria, stimulating immune cells to recruit and activate a superior lymphocyte population. Simply put, LPS from micrococcal outer membrane lipids can stimulate the expression of antimicrobial peptides in skin keratinocytes, activating the skin's defense system without the inherent defects associated with pathogens or intact bacteria. Micrococcal fermentation broth can positively impact the balance of the skin's microecological ecosystem. It is hypothesized that LPS from one or more lipids in the micrococcus can stimulate the expression of antimicrobial peptides in skin keratinocytes, thereby increasing the skin's resistance to external stresses.

[0021] ASM Cytokinin (containing micrococcal lysate; purchased from Chongqing Yasu Biotechnology Co., Ltd.) combats skin dryness and sensitivity, restores the skin barrier, and improves the diversity of skin surface microorganisms. Based on this microecological antimicrobial mechanism, ASM Cytokinin can be used to: limit seborrheic conditions, particularly the proliferation of pathogenic skin microorganisms that contribute to oily skin or scalp; prevent or limit dandruff symptoms; improve oral hygiene; regulate intimate microbial balance; and limit unpleasant odors associated with skin microbial proliferation in confined areas.

[0022] The present invention promotes the growth and function of beneficial bacteria by combining a synergistic system of plant active ingredients (hydrolyzed ginsenosides (anti-oxidation, reducing environmental damage, anti-oxidation, anti-inflammatory, promoting type I collagen, preventing hair loss and growth), micrococcal lysate (building skin microecology, soothing and repairing, promoting skin repair), Asian birch sap (long-lasting moisturizing base, antibacterial and antibacterial, soothing and anti-allergic)) and prebiotics (acetyl glucosamine (strengthening and repairing barrier, promoting hyaluronic acid, removing waste keratin, promoting skin renewal), fermented tremella polysaccharide (regulating sebum balance, moisturizing, improving fine lines, anti-oxidation, anti-allergic)). Inhibiting harmful bacteria (such as Malassezia, Staphylococcus aureus, etc.) enhances skin homeostasis, helps maintain the integrity of the skin barrier, promotes the normal metabolism of the stratum corneum, helps avoid the disorder of the skin microecology caused by improper skin cleaning (overcleaning or insufficient cleaning), the influence of the external environment (such as ultraviolet rays), irritating products (such as hormones, fruit acids), etc., helps avoid inflammation and infection caused by reduced skin resistance due to imbalance of the flora, as well as abnormal skin sensitivity and repeated acne, and helps avoid symptoms such as dryness, tightness, itching, and various skin diseases such as allergic dermatitis and psoriasis due to weakened skin barrier function. In addition, the present invention can also avoid water-oil imbalance and achieve the effect of oil control.

[0023] In some embodiments, the mass ratio of the hydrolyzed ginsenosides, micrococcal lysate, acetyl glucosamine, birch sap, and tremella polysaccharide is (0.01-0.03):(3-5.1):(0.5-1.5):(70-88):(0.05-0.15). In some embodiments, the mass ratio of the hydrolyzed ginsenosides, micrococcal lysate, acetyl glucosamine, birch sap, and tremella polysaccharide is (0.01-0.03):(3-5.0025):(0.5-1.5):(70.81625-87.60375):(0.05-0.15). In some embodiments, the mass ratio of the hydrolyzed ginsenosides, micrococcal lysate, acetyl glucosamine, Asian birch sap and Tremella polysaccharide is 0.01:3:0.5:87.60375:0.05, or 0.02:3.75:1:79.4575:0.1, or 0.03:5.0025:1.5:70.81625:0.15.

[0024] In some embodiments, the composition further comprises a solvent.

[0025] In some embodiments, the solvent includes at least one of 1,3-butanediol, 1,2-hexanediol, and water.

[0026] In some embodiments, the solvent includes 1,3-butanediol, 1,2-hexanediol, and water.

[0027] In some embodiments, the composition further comprises other ingredients.

[0028] In some embodiments, the other ingredients include at least one of C12-13 pareth-9, 1,2-pentanediol, and p-hydroxyacetophenone.

[0029] In some embodiments, the content of the hydrolyzed ginsenosides is 0.01 wt% to 0.03 wt% based on the total weight of the composition. In some embodiments, the content of the hydrolyzed ginsenosides is 0.01 wt%, 0.02 wt%, or 0.03 wt% based on the total weight of the composition.

[0030] In some embodiments, the content of the micrococcal lysate is 3.0 wt%-5.1 wt% based on the total weight of the composition. In some embodiments, the content of the micrococcal lysate is 3.0 wt%-5.0025 wt% based on the total weight of the composition. In some embodiments, the content of the micrococcal lysate is 3.0 wt%, 3.75 wt%, or 5.0025 wt% based on the total weight of the composition.

[0031] In some embodiments, the content of the acetyl glucosamine is 0.5 wt%-1.5 wt% based on the total mass of the composition. In some embodiments, the content of the acetyl glucosamine is 0.5 wt%, 1.0 wt% or 1.5 wt% based on the total mass of the composition.

[0032] In some embodiments, the content of the Asian birch sap is 70wt%-90wt% based on the total mass of the composition. In some embodiments, the content of the Asian birch sap is 70wt%-87.7wt% based on the total mass of the composition. In some embodiments, the content of the Asian birch sap is 70.8wt%, 79.5wt% or 87.6wt% based on the total mass of the composition. In some embodiments, the content of the Asian birch sap is 70wt%, 70.81625wt%, 71wt%, 72wt%, 73wt%, 74wt%, 75wt%, 76wt%, 77wt%, 78wt%, 79wt%, 79.4575wt%, 80wt%, 81wt%, 82wt%, 83wt%, 84wt%, 85wt%, 86wt%, 87wt% or 87.60375wt%.

[0033] In some embodiments, the Tremella polysaccharide content is 0.05wt%-0.15wt% based on the total mass of the composition. In some embodiments, the Tremella polysaccharide content is 0.05wt%, 0.1wt% or 0.15wt% based on the total mass of the composition.

[0034] In some embodiments, the content of the solvent is 8.0 wt% to 27.6 wt% based on the total weight of the composition. In some embodiments, the content of the solvent is 8.4 wt% to 22.0 wt% based on the total weight of the composition. In some embodiments, the content of the solvent is 8.0 wt%, 8.1 wt%, 8.2 wt%, 8.3 wt%, 8.4 wt%, 8.4275 wt%, 8.5 wt%, 8.6 wt%, 8.7 wt%, 8.8 wt%, 8.9 wt%, 9.0 wt%, 9.5 wt%, 10 wt%, 11 wt%, 12 wt%, 13 wt%, 14 wt%, 15 wt%, 15.155 wt%, 15.2 wt%, 15.5 wt%, 16 wt%, 17 wt%, 18 wt%, 19 wt%, 20 wt%, 21 wt%, 21.875 wt%, 22 wt%, 23 wt%, 24 wt%, 25 wt%, 26 wt%, 27 wt% or 27.6 wt% based on the total mass of the composition. In some embodiments, based on the total mass of the composition, the content of the solvent is 8.4275 wt%, 15.155 wt% or 21.875 wt%.

[0035] In some embodiments, the 1,3-butanediol content is 3 wt% to 7 wt% based on the total weight of the composition. In some embodiments, the 1,3-butanediol content is 3 wt% to 6.9925 wt% based on the total weight of the composition. In some embodiments, the 1,3-butanediol content is 3 wt%, 3.5 wt%, 4 wt%, 4.5 wt%, 5 wt%, 5.5 wt%, 6 wt%, 6.5 wt%, 6.9925 wt%, or 7 wt% based on the total weight of the composition.

[0036] In some embodiments, the 1,2-hexanediol content is 0.4 wt % to 0.6 wt % based on the total weight of the composition. In some embodiments, the 1,2-hexanediol content is 0.4 wt %, 0.45 wt %, 0.5 wt %, 0.55 wt % or 0.6 wt % based on the total weight of the composition.

[0037] In some embodiments, the content of 1,2-pentanediol is 0 wt%-0.015 wt% based on the total weight of the composition. In some embodiments, the content of 1,2-pentanediol is 0 wt%, 0.001 wt%, 0.002 wt%, 0.003 wt%, 0.00375 wt%, 0.004 wt%, 0.005 wt%, 0.006 wt%, 0.007 wt%, 0.0075 wt%, 0.008 wt%, 0.009 wt%, 0.010 wt%, 0.011 wt% or 0.01125 wt% based on the total weight of the composition. In some embodiments, the content of 1,2-pentanediol is 0.00375 wt%, 0.0075 wt% or 0.01125 wt% based on the total weight of the composition.

[0038] In some embodiments, the water content is 4wt%-20wt% based on the total mass of the composition. In some embodiments, the water content is 4wt%-15wt% based on the total mass of the composition. In some embodiments, the water content is 4wt%, 4.5wt%, 4.6wt%, 4.7wt%, 4.8wt%, 4.8275wt%, 4.9wt%, 5wt%, 5.5wt%, 6wt%, 6.5wt%, 7wt%, 7.5wt%, 8wt%, 8.5wt%, 9wt%, 9.5wt%, 9.655wt%, 10wt%, 10.5wt%, 11wt%, 11.5wt%, 12wt%, 12.5wt%, 13wt%, 13.5wt%, 14wt% or 14.4825wt%.

[0039] In some embodiments, the content of p-hydroxyacetophenone is 0.4 wt % to 0.6 wt % based on the total weight of the composition. In some embodiments, the content of p-hydroxyacetophenone is 0.4 wt %, 0.45 wt %, 0.5 wt %, 0.55 wt % or 0.6 wt % based on the total weight of the composition.

[0040] In some embodiments, the content of the C12-13 pareth-9 is 0.005 wt% to 0.015 wt% based on the total weight of the composition. In some embodiments, the content of the C12-13 pareth-9 is 0.005 wt%, 0.01 wt% or 0.015 wt% based on the total weight of the composition.

[0041] In some embodiments, based on the total mass of the composition, the content of the hydrolyzed ginsenosides is 0.01wt%-0.03wt%, the content of the micrococcal lysate is 3.0wt%-5.1wt%, the content of the acetyl glucosamine is 0.5wt%-1.5wt%, the content of the Asian birch sap is 70wt%-87.7wt%, the content of the Tremella polysaccharide is 0.05wt%-0.15wt%, the content of the solvent is 8.4wt%-22.0wt%, the content of p-hydroxyacetophenone is 0.4wt%-0.6wt%, and the content of the C12-13 paraether-9 is 0.005wt%-0.015wt%.

[0042] In some embodiments, calculated based on the total mass of the composition, the content of the hydrolyzed ginsenosides is 0.01wt%-0.03wt%, the content of the micrococcal lysate is 3.0wt%-5.0025wt%, the content of the acetyl glucosamine is 0.5wt%-1.5wt%, the content of the Asian birch sap is 70.81625wt%-87.60375wt%, the content of the Tremella polysaccharide is 0.05wt%-0.15wt%, the content of the p-hydroxyacetophenone is 0.4wt%-0.6wt%, the content of the C12-13 paraether-9 is 0.005wt%-0.015wt%, and the content of the solvent is 8.4275wt%-21.875wt%.

[0043] In some embodiments, calculated based on the total mass of the composition, the content of the hydrolyzed ginsenosides is 0.01wt%-0.03wt%, the content of the micrococcal lysate is 3.0wt%-5.0025wt%, the content of the acetyl glucosamine is 0.5wt%-1.5wt%, the content of the tremella polysaccharide is 0.05wt%-0.15wt%, the content of the p-hydroxyacetophenone is 0.4wt%-0.6wt%, the content of the C12-13 paraether-9 is 0.005wt%-0.015wt%, the content of the solvent is 8.4275wt%-21.875wt%, and the balance is the Asian birch sap.

[0044] In some embodiments, based on the total mass of the composition, the content of the hydrolyzed ginsenosides is 0.01wt%-0.03wt%, the content of the micrococcal lysate is 3.0wt%-5.0025wt%, the content of acetyl glucosamine is 0.5wt%-1.5wt%, the content of Tremella polysaccharide is 0.05wt%-0.15wt%, the content of p-hydroxyacetophenone is 0.4wt%-0.6wt%, and the C1 The content of 2-13 paraffin polyether-9 is 0.005wt%-0.015wt%, the content of 1,3-butanediol is 3wt%-6.9925wt%, the content of 1,2-hexanediol is 0.4wt%-0.6wt%, the content of 1,2-pentanediol is 0.00375wt%-0.01125wt%, the content of water is 4.8275wt%-14.4825wt%, and the balance is the Asian birch sap.

[0045] In some embodiments, calculated based on the total mass of the composition, the content of the hydrolyzed ginsenosides is 0.01wt%, the content of the micrococcal lysate is 3.0wt%, the content of the acetyl glucosamine is 0.5wt%, the content of the tremella polysaccharide is 0.05wt%, the content of the p-hydroxyacetophenone is 0.4wt%, the content of the C12-13 paraether-9 is 0.005wt%, the content of the 1,3-butanediol is 3wt%, the content of the 1,2-hexanediol is 0.6wt%, the content of the 1,2-pentanediol is 0.00375wt%, the content of the water is 4.8275wt%, and the balance is the Asian white birch sap.

[0046] In some embodiments, calculated based on the total mass of the composition, the content of the hydrolyzed ginsenosides is 0.02wt%, the content of the micrococcal lysate is 3.75wt%, the content of the acetyl glucosamine is 1wt%, the content of the tremella polysaccharide is 0.1wt%, the content of p-hydroxyacetophenone is 0.5wt%, the content of C12-13 paraether-9 is 0.01wt%, the content of 1,3-butanediol is 5wt%, the content of 1,2-hexanediol is 0.5wt%, the content of 1,2-pentanediol is 0.0075wt%, the content of water is 9.655wt%, and the balance is the Asian white birch sap.

[0047] In some embodiments, calculated based on the total mass of the composition, the content of the hydrolyzed ginsenosides is 0.03wt%, the content of the micrococcal lysate is 5.0025wt%, the content of the acetyl glucosamine is 1.5wt%, the content of the Tremella polysaccharide is 0.15wt%, the content of the p-hydroxyacetophenone is 0.6wt%, the content of the C12-13 paraether-9 is 0.015wt%, the content of the 1,3-butanediol is 6.9925wt%, the content of the 1,2-hexanediol is 0.4wt%, the content of the 1,2-pentanediol is 0.01125wt%, the content of the water is 14.4825wt%, and the balance is the Asian white birch sap.

[0048] In some embodiments, calculated based on the total mass of the composition, the content of the hydrolyzed ginsenosides is 0.02wt%, the content of the micrococcal lysate is 1.25wt%, the content of the acetyl glucosamine is 1wt%, the content of the tremella polysaccharide is 0.1wt%, the content of the 1,3-butanediol is 5wt%, the content of the 1,2-hexanediol is 0.5wt%, the content of the p-hydroxyacetophenone is 0.5wt%, the content of the C12-13 paraether-9 is 0.01wt%, the content of the water is 15wt%, and the balance is Asian birch sap.

[0049] In some embodiments, the composition comprises rare ginsenoside PLF, ASM cytokinin, acetyl glucosamine, fermented Tremella polysaccharide Trepoly-01 and Asian white birch (BETULA PLATYPHYLLA) sap, and optionally at least one selected from 1,3-butanediol, 1,2-hexanediol and p-hydroxyacetophenone;

[0050] The rare ginsenoside PLF is composed of hydrolyzed ginsenosides, water, C12-13 parether-9, 1,3-butanediol, 1,2-pentanediol and 1,2-hexanediol, wherein the content of the hydrolyzed ginsenosides in the rare ginsenoside PLF is 3wt%-5wt%, the content of the C12-13 parether-9 in the rare ginsenoside PLF is 1.8wt%-2.2wt%, the content of the 1,3-butanediol in the rare ginsenoside PLF is 9wt%-11wt%, the content of the 1,2-pentanediol in the rare ginsenoside PLF is 1.3wt%-1.7wt%, the content of the 1,2-hexanediol in the rare ginsenoside PLF is 1.3wt%-1.7wt%, and the content of water in the rare ginsenoside PLF is 79wt%-83wt%;

[0051] The ASM cytokinin is composed of micrococcal lysate and 1,3-butanediol, wherein the content of the micrococcal lysate in the ASM cytokinin is 70wt%-78wt%, and the balance is 1,3-butanediol;

[0052] The fermented tremella polysaccharide Trepoly-01 consists of tremella polysaccharide, 1,3-butanediol, 1,2-hexanediol, p-hydroxyacetophenone and water, wherein the content of the tremella polysaccharide in the fermented tremella polysaccharide is 0.9wt%-1.1wt%, the content of the 1,3-butanediol in the fermented tremella polysaccharide is 2wt%-8wt%, the content of the 1,2-hexanediol in the fermented tremella polysaccharide is 0.5wt%-1wt%, the content of the p-hydroxyacetophenone in the fermented tremella polysaccharide is 0.2wt%-0.8wt%, and the content of the water in the fermented tremella polysaccharide is 89.1wt%-96.4wt%.

[0053] In some embodiments, the rare ginsenoside PLF is composed of hydrolyzed ginsenosides, water, C12-13 pareth-9, 1,3-butanediol, 1,2-pentanediol and 1,2-hexanediol, wherein the content of the hydrolyzed ginsenosides in the rare ginsenoside PLF is 4wt%, the content of the C12-13 pareth-9 in the rare ginsenoside PLF is 2wt%, the content of the 1,3-butanediol in the rare ginsenoside PLF is 10wt%, the content of the 1,2-pentanediol in the rare ginsenoside PLF is 1.5wt%, the content of the 1,2-hexanediol in the rare ginsenoside PLF is 1.5wt%, and the content of water in the rare ginsenoside PLF is 81wt%.

[0054] In some embodiments, the ASM cytokinin is composed of micrococcal lysate and 1,3-butanediol, wherein the content of the micrococcal lysate in the ASM cytokinin is 75 wt % and the balance is 1,3-butanediol.

[0055] In some embodiments, the fermented tremella polysaccharide Trepoly-01 is composed of tremella polysaccharide, 1,3-butanediol, 1,2-hexanediol, p-hydroxyacetophenone and water, wherein the content of the tremella polysaccharide in the fermented tremella polysaccharide is 1wt%, the content of the 1,3-butanediol in the fermented tremella polysaccharide is 5wt%, the content of the 1,2-hexanediol in the fermented tremella polysaccharide is 1wt%, the content of the p-hydroxyacetophenone in the fermented tremella polysaccharide is 0.5wt%, and the content of the water in the fermented tremella polysaccharide is 92.5wt%.

[0056] In a second aspect, the present invention provides a method for preparing the composition described in the first aspect.

[0057] In some embodiments, a method for preparing the composition of the first aspect comprises: mixing the active ingredient and a solvent, or mixing the active ingredient, a solvent and other ingredients, and stirring until completely dissolved and transparent to obtain the composition.

[0058] In some embodiments, a method for preparing the composition of the first aspect is characterized by comprising: taking rare ginsenoside PLF, ASM cytokinin, acetyl glucosamine, fermented tremella polysaccharide Trepoly-01 and Asian white birch (BETULAPLATYPHYLLA) sap, and optionally at least one selected from 1,3-butanediol, 1,2-hexanediol and parahydroxyacetophenone, mixing, and stirring until completely dissolved and transparent to obtain the composition.

[0059] In some embodiments, the rare ginsenoside PLF is composed of hydrolyzed ginsenosides, water, C12-13 pareth-9, 1,3-butanediol, 1,2-pentanediol and 1,2-hexanediol, wherein the content of the hydrolyzed ginsenosides in the rare ginsenoside PLF is 3wt%-5wt%, the content of the C12-13 pareth-9 in the rare ginsenoside PLF is 1.8wt%-2.2wt%, the content of the 1,3-butanediol in the rare ginsenoside PLF is 9wt%-11wt%, the content of the 1,2-pentanediol in the rare ginsenoside PLF is 1.3wt%-1.7wt%, the content of the 1,2-hexanediol in the rare ginsenoside PLF is 1.3wt%-1.7wt%, and the content of water in the rare ginsenoside PLF is 79wt%-83wt%.

[0060] In some embodiments, the ASM cytokinin is composed of micrococcal lysate and 1,3-butanediol, wherein the content of the micrococcal lysate in the ASM cytokinin is 70 wt %-78 wt %, and the balance is 1,3-butanediol.

[0061] In some embodiments, the fermented tremella polysaccharide Trepoly-01 is composed of tremella polysaccharide, 1,3-butanediol, 1,2-hexanediol, p-hydroxyacetophenone and water, wherein the content of the tremella polysaccharide in the fermented tremella polysaccharide is 0.9wt%-1.1wt%, the content of the 1,3-butanediol in the fermented tremella polysaccharide is 2wt%-8wt%, the content of the 1,2-hexanediol in the fermented tremella polysaccharide is 0.5wt%-1wt%, the content of the p-hydroxyacetophenone in the fermented tremella polysaccharide is 0.2wt%-0.8wt%, and the content of the water in the fermented tremella polysaccharide is 89.1wt%-96.4wt%.

[0062] In some embodiments, the rare ginsenoside PLF is composed of hydrolyzed ginsenosides, water, C12-13 pareth-9, 1,3-butanediol, 1,2-pentanediol and 1,2-hexanediol, wherein the content of the hydrolyzed ginsenosides in the rare ginsenoside PLF is 4wt%, the content of the C12-13 pareth-9 in the rare ginsenoside PLF is 2wt%, the content of the 1,3-butanediol in the rare ginsenoside PLF is 10wt%, the content of the 1,2-pentanediol in the rare ginsenoside PLF is 1.5wt%, the content of the 1,2-hexanediol in the rare ginsenoside PLF is 1.5wt%, and the content of water in the rare ginsenoside PLF is 81wt%.

[0063] In some embodiments, the ASM cytokinin is composed of micrococcal lysate and 1,3-butanediol, wherein the content of the micrococcal lysate in the ASM cytokinin is 75 wt % and the balance is 1,3-butanediol.

[0064] In some embodiments, the fermented tremella polysaccharide Trepoly-01 is composed of tremella polysaccharide, 1,3-butanediol, 1,2-hexanediol, p-hydroxyacetophenone and water, wherein the content of the tremella polysaccharide in the fermented tremella polysaccharide is 1wt%, the content of the 1,3-butanediol in the fermented tremella polysaccharide is 5wt%, the content of the 1,2-hexanediol in the fermented tremella polysaccharide is 1wt%, the content of the p-hydroxyacetophenone in the fermented tremella polysaccharide is 0.5wt%, and the content of the water in the fermented tremella polysaccharide is 92.5wt%.

[0065] In a third aspect, the present invention provides a cosmetic or cleaning product.

[0066] A cosmetic or cleaning product comprising the composition of the first aspect or the composition prepared by the preparation method of the second aspect;

[0067] In some embodiments, the cosmetics or cleaning products are used for anti-oxidation, anti-inflammation, skin repair, antibacterial and antibacterial, soothing and anti-allergic, promoting hyaluronic acid production, exfoliating dead skin cells, promoting skin renewal, moisturizing, improving fine lines, repairing hair, nourishing hair, anti-dandruff and / or oil control.

[0068] In some embodiments, the cosmetic further comprises other cosmetic active substances (such as functional additives, active extracts, etc.) and / or cosmetic bases.

[0069] In some embodiments, the cleaning product further comprises other active substances for cleaning products and / or a base for cleaning products.

[0070] In some embodiments, the content of the composition in the cosmetics or cleaning products is 0.01wt%-2wt%. In some embodiments, the content of the composition in the cosmetics or cleaning products is 0.1wt%-2wt%. In some embodiments, the content of the composition in the cosmetics or cleaning products is 0.15wt%-2wt%. In some embodiments, the content of the composition in the cosmetics or cleaning products is 0.01wt%, 0.05wt%, 0.1wt%, 0.15wt%, 0.2wt%, 0.25wt%, 0.3wt%, 0.4wt%, 0.5wt%, 0.6wt%, 0.7wt%, 0.8wt%, 0.9wt%, 1wt%, 1.5wt% or 2wt%.

[0071] In a fourth aspect, the present invention provides an application of the composition described in the first aspect or the composition prepared by the preparation method of the second aspect.

[0072] Use of the composition of the first aspect or the composition prepared by the preparation method of the second aspect in the preparation of cosmetics or cleaning products;

[0073] In some embodiments, the cosmetics or cleaning products are used for anti-oxidation, anti-inflammation, skin repair, antibacterial and antibacterial, soothing and anti-allergic, promoting hyaluronic acid production, exfoliating dead skin cells, promoting skin renewal, moisturizing, improving fine lines, repairing hair, nourishing hair, anti-dandruff and / or oil control.

[0074] In some embodiments, the cosmetic or cleaning product is selected from the following forms of cosmetics or cleaning products: liquid, cream, hand cream, serum, gel, bar, spray, ointment, liquid detergent, soap bar, shower gel, facial cleanser, shampoo, conditioner, hair oil, body lotion, hand cream, paste, foam, powder, mousse, hydrogel, film-forming product, facial mask, hair mask.

[0075] In some embodiments, the cosmetic further comprises other cosmetic active substances (such as functional additives, active extracts, etc.) and / or cosmetic bases.

[0076] In certain embodiments, the cleaning products also include other cleaning products active substances and / or cleaning products matrix.In certain embodiments, the content of the composition in the cosmetics or cleaning products is 0.01wt%-2wt%.In certain embodiments, the content of the composition in the cosmetics or cleaning products is 0.1wt%-2wt%.In certain embodiments, the content of the composition in the cosmetics or cleaning products is 0.15wt%-2wt%.In certain embodiments, the content of the composition in the cosmetics or cleaning products is 0.01wt%, 0.05wt%, 0.1wt%, 0.15wt%, 0.2wt%, 0.25wt%, 0.3wt%, 0.4wt%, 0.5wt%, 0.6wt%, 0.7wt%, 0.8wt%, 0.9wt%, 1wt%, 1.5wt% or 2wt%.

[0077] Beneficial effects

[0078] Compared with the prior art, a certain embodiment of the present invention has at least one of the following beneficial effects:

[0079] (1) The present invention promotes the reproduction of beneficial bacteria by regulating the bacterial environment through the synergistic system of compound plant active ingredients (hydrolyzed ginsenosides (anti-oxidation, reducing environmental damage, anti-oxidation, anti-inflammatory, promoting type I collagen, preventing hair loss and growth), micrococcal lysate (building skin microecology, soothing and repairing, promoting skin repair), Asian birch sap (long-lasting moisturizing base, antibacterial and antibacterial, soothing and anti-allergic)) and prebiotics (acetyl glucosamine (strengthening and repairing barrier, promoting hyaluronic acid, removing waste keratin, promoting skin renewal), fermented Tremella polysaccharide (regulating sebum balance, moisturizing, improving fine lines, anti-oxidation, anti-allergic)). The invention can also inhibit harmful bacteria (such as Malassezia, Staphylococcus aureus, etc.) to enhance skin homeostasis, maintain the integrity of the skin barrier, promote the normal metabolism of the stratum corneum, avoid the disorder of the skin microecology caused by improper skin cleaning (over-cleaning or insufficient cleaning), the influence of the external environment (such as ultraviolet rays), irritating products (such as hormones, fruit acids), etc., and avoid inflammation and infection caused by reduced skin resistance due to imbalance of the bacterial flora, as well as abnormal skin sensitivity and repeated acne. It is also conducive to avoiding the symptoms of dryness, tightness, itching, and various skin diseases such as allergic dermatitis and psoriasis due to weakened skin barrier function. In addition, the present invention can also avoid water-oil imbalance and achieve the effect of oil control.

[0080] (2) The hair mask comprising the composition provided by the present invention containing hydrolyzed ginsenosides, micrococcal lysate, acetyl glucosamine, tremella polysaccharide and Asian birch sap has excellent hair repairing and nourishing effects.

[0081] (3) The composition provided by the present invention, which contains hydrolyzed ginsenosides, micrococcal lysate, acetyl glucosamine, tremella polysaccharide and Asian birch sap, synergistically improves the soothing, hair repairing and nourishing effects of the hydrolyzed ginsenosides, micrococcal lysate, acetyl glucosamine, tremella polysaccharide and Asian birch sap, and has an unexpected synergistic technical effect.

[0082] (4) The shower gel or shampoo comprising the composition provided by the present invention has good soothing effect, moisturizing effect, oil control effect, and anti-dandruff effect, and is mild and non-irritating, and has excellent technical effects.

[0083] Terminology

[0084] In the description of the present invention, “plurality” means two or more, unless otherwise clearly defined.

[0085] The term "room temperature" means ambient temperature, which refers to a temperature between about 10°C and about 30°C, or between about 20°C and 30°C, or about 25°C.

[0086] The term "wt%" means percentage by mass.

[0087] The term "balance" means that the sum of the contents of the various components added to a mixture (such as the composition, hair mask, body wash or shampoo of the present invention) is 100 wt%.

[0088] "Cosmetic matrix" refers to the basic material system (such as oil-based matrix) used to carry and stabilize other ingredients in cosmetics, in addition to active ingredients (such as functional additives, active extracts, etc.), and to give the product a specific form (such as liquid, cream, hand cream, essence, gel, paste, spray, ointment, liquid detergent, soap bar, shower gel, facial cleanser, shampoo, conditioner, hair care essential oil, body lotion, hand cream, paste, foam, powder, mousse, hydrogel, film-forming products, facial mask, hair mask, etc.), texture and feel of use. The cosmetic base shall comply with the requirements of the relevant national laws, regulations and standards (such as the "Technical Specifications for Safety of Cosmetics").

[0089] "Cleaning product matrix" refers to the basic substance combination in cleaning products (such as detergents, dishwashing liquids, shampoos, shower gels, etc.), in addition to specific cleaning active ingredients (such as surfactants, etc.) and functional additives, which is used to carry and stabilize the formula system and give the product a specific physical form (such as liquid, solid, paste), cleaning performance and usage experience (such as solvent matrix (such as water, ethanol, 1,2-propylene glycol, 1,3-butylene glycol, etc.), builder matrix (such as sodium tripolyphosphate, sodium citrate, zeolite, etc.), thickener matrix (such as sodium chloride, carbomer, cellulose ether, etc.), foam regulating matrix (such as cocamidopropyl betaine, sodium laureth sulfate, etc.), pH regulating matrix (such as citric acid, sodium hydroxide, sodium bicarbonate, etc.), functional auxiliary matrix (such as enzyme preparations (protease, lipase), abrasive (silicon dioxide), etc.)).

[0090] In the description of this specification, the reference terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" mean that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art can combine and combine different embodiments or examples described in this specification and features of different embodiments or examples without contradiction.

[0091] In the following disclosure, all numerical values disclosed herein are approximate, regardless of whether the word "about" or "approximately" is used. The numerical value of each number may vary by 1%, 2%, 5%, 7%, 8%, 10%, 15%, or 20%. Whenever a number having a value of N is disclosed, any number having a value of N+ / -1%, N+ / -2%, N+ / -3%, N+ / -5%, N+ / -7%, N+ / -8%, N+ / -10%, N+ / -15%, or N+ / -20% is expressly disclosed, where "+ / -" means plus or minus. BRIEF DESCRIPTION OF THE DRAWINGS

[0092] Figure 1 This is a statistical curve diagram of the moisturizing rate of the shampoo in Example 2 (the curve of the test sample) and the positive control in Test Example 3. DETAILED DESCRIPTION

[0093] In order to enable those skilled in the art to better understand the technical solutions of the present invention, some non-limiting embodiments are further disclosed below to further illustrate the present invention in detail.

[0094] The reagents used in the present invention can be purchased from the market or prepared by the method described in the present invention.

[0095] 1. The sources of some of the reagents used in the embodiments or comparative examples of the present invention are as follows:

[0096] Asian white birch sap: Trade name: birch water, wherein the Asian white birch (BETULA PLATYPHYLLA) sap content is 100%, purchased from Yichun Jiahua Biotechnology Co., Ltd.

[0097] Suni Jinsenside PLF: Composed of hydrolyzed ginsenosides (HYDROLYZEDGINSENG SAPONINS), water, C12-13 pareth-9, 1,3-butylene glycol, 1,2-pentanediol and 1,2-hexanediol, wherein the content of the hydrolyzed ginsenosides in the rare ginsenoside PLF is 3wt%-5wt% (typical value or target value: 4wt%), the content of the C12-13 pareth-9 in the rare ginsenoside PLF is 1.8wt%-2.2wt% (typical value or target value: 2wt%), and the content of the 1,3-butylene glycol in the rare ginsenoside PLF is 9wt%. -11wt% (typical value or target value: 10wt%), the content of 1,2-pentanediol in the rare ginsenoside PLF is 1.3wt%-1.7wt% (typical value or target value: 1.5wt%), the content of 1,2-hexanediol in the rare ginsenoside PLF is 1.3wt%-1.7wt% (typical value or target value: 1.5wt%), and the content of water in the rare ginsenoside PLF is 79wt%-83wt% (typical value or target value: 81wt%); purchased from Sanyi Technology (Guangzhou) Co., Ltd.

[0098] ASM cytokinin: composed of 70wt%-78wt% (typical value or target value: 75wt%) of micrococcal lysate and 22wt%-30wt% (typical value or target value: 25wt%) of 1,3-butanediol; purchased from Chongqing Yasu Biotechnology Co., Ltd.

[0099] Fermented Tremella polysaccharide Trepoly-01: composed of Tremella fuciformis polysaccharide, 1,3-butanediol, 1,2-hexanediol, p-hydroxyacetophenone and water. The content of FUCIFORMIS (Trench Fungus) polysaccharide in the fermented Tremella polysaccharide is 0.9wt%-1.1wt% (typical value or target value: 1wt%), the content of 1,3-butanediol in the fermented Tremella polysaccharide is 2wt%-8wt% (typical value or target value: 5wt%), the content of 1,2-hexanediol in the fermented Tremella polysaccharide is 0.5wt%-1wt% (typical value or target value: 1wt%), the content of p-hydroxyacetophenone in the fermented Tremella polysaccharide is 0.2wt%-0.8wt% (typical value or target value: 0.5wt%), and the content of water in the fermented Tremella polysaccharide is 89.1wt%-96.4wt% (typical value or target value: 92.5wt%); purchased from Guangdong Been Biotechnology Co., Ltd.

[0100] Example 1-Example 3: Preparation of composition

[0101] Formula: The raw material formula is shown in Table 1, and the final component content is shown in Table 2.

[0102] Table 1: Raw material formula

[0103]

[0104] Table 2: Final component contents of Examples 1-3

[0105]

[0106] Preparation method: Mix the components according to the formula in Table 1 and stir until they are completely dissolved and transparent to obtain the composition.

[0107] Formulation: Based on the formulation of Example 2, the missing components were investigated, and the final component contents are shown in Table 3.

[0108] Table 3: Final component contents of Comparative Examples 1-5

[0109]

[0110] Preparation method: The content of the raw material containing the active ingredient, other ingredients to be added, and solvent to be added are calculated according to the final active ingredient content described in Table 3, and the content of each active component raw material, other ingredients to be added, and solvent to be added are mixed to obtain the composition.

[0111] Comparative Examples 6-11: Single active ingredient or no active ingredient formulation: Single active ingredient or no active ingredient formulation was investigated based on the formulation of Example 2. The final component contents are shown in Table 4.

[0112] Table 4: Final composition of Comparative Examples 6-11

[0113]

[0114] Preparation method: The content of the raw material containing the active ingredient, the other ingredients to be added, and the solvent to be added are calculated according to the final active ingredient content described in Table 4, and the content of the raw material of each active component, the other ingredients to be added, and the solvent to be added are mixed to obtain the composition.

[0115] Example 4: Shampoo

[0116] Recipe: See Table 5.

[0117] Table 5: Shampoo formula

[0118]

[0119]

[0120] Preparation method:

[0121] Step 1: Accurately weigh water, guar hydroxypropyltrimonium chloride, carbomer, ammonium lauryl sulfate, sodium cocoyl glycinate, cocamide MEA, cocamide DEA, polyquaternium-7, zinc chloride, hyaluronic acid, disodium EDTA, and piroctone olamine salt in an emulsifier and heat with stirring until completely dissolved at 85°C. Keep warm for 30 minutes and then cool.

[0122] Step 2: Stir and cool down. When the temperature drops to 45°C, add sodium chloride and stir evenly. The stirring speed is 20 r / min.

[0123] Step 3: Stir and cool down. When the temperature drops to 40°C, add hydrolyzed silk protein, DC-1785, DC-7137, DC-949, macadamia nut (MACADAMIA TERNIFOLIA) seed oil, avocado tree (BUTYROSPERMUM PARKII) fruit butter, cherry blossom extract, camellia extract, ginger root extract, anti-hair loss and growth liquid, ginseng enzyme, panthenol, tocopheryl acetate, combination, essence, bisabolol, niacinamide, arginine, WSK (tremella heteropolysaccharide), and phenoxyethanol and stir evenly at a stirring speed of 20 r / min.

[0124] .Step 4: Take samples for testing, filter out the material after passing the test, and obtain shampoo.

[0125] The compositions of Examples 1 to 3 and Comparative Examples 1 to 11 were respectively prepared according to the above method to obtain shampoos of different Examples or Comparative Examples, which were respectively named as: Example 1-Shampoo, Example 2-Shampoo, Example 3-Shampoo, Comparative Example 1-Shampoo, Comparative Example 2-Shampoo, Comparative Example 3-Shampoo, Comparative Example 4-Shampoo, Comparative Example 5-Shampoo, Comparative Example 6-Shampoo, Comparative Example 7-Shampoo, Comparative Example 8-Shampoo, Comparative Example 9-Shampoo, Comparative Example 10-Shampoo and Comparative Example 11-Shampoo.

[0126] Example 5: Hair Mask

[0127] Recipe: See Table 6.

[0128] Table 6: Hair mask formula

[0129]

[0130]

[0131] Preparation method:

[0132] Step 1: Accurately weigh water, disodium EDTA, sodium benzoate, citric acid, sodium hyaluronate, hydroxyethylcellulose, stearyltrimethylammonium chloride, trehalose, and glycerin in an emulsifier and heat and stir until completely dissolved at 85°C. Keep warm for 30 minutes and then cool down.

[0133] Step 2: Accurately weigh behentrimonium methylsulfate, cetearyl alcohol, behenamidopropyl dimethylamine, camellia seed oil, tocopheryl acetate, squalane, and dimethicone in an emulsifier. Heat and stir until completely dissolved at 85°C. Add to the emulsifier and homogenize for 3-5 minutes. Keep warm for 30 minutes before cooling.

[0134] Step 2: Stir and cool. When the temperature drops to 40°C, add panthenol, DC949, phenoxyethanol, the mixture, macadamia (MACADAMIA TERNIFOLIA) seed oil, avocado (BUTYROSPERMUM PARKII) butter, silk protein, collagen, WSK (Tremella fuciformis polysaccharide), ceramide, and fragrance and mix well.

[0135] Step 4: Filter the material to obtain a hair mask.

[0136] The compositions of Examples 1 to 3 and Comparative Examples 1 to 11 were respectively prepared according to the above method to obtain hair masks of different Examples or Comparative Examples, which were respectively named: Example 1-hair mask, Example 2-hair mask, Example 3-hair mask, Comparative Example 1-hair mask, Comparative Example 2-hair mask, Comparative Example 3-hair mask, Comparative Example 4-hair mask, Comparative Example 5-hair mask, Comparative Example 6-hair mask, Comparative Example 7-hair mask, Comparative Example 8-hair mask, Comparative Example 9-hair mask, Comparative Example 10-hair mask, and Comparative Example 11-hair mask.

[0137] Example 6: Shower Gel

[0138] Recipe: See Table 7.

[0139] Table 7: Body wash formula

[0140]

[0141]

[0142] Preparation method:

[0143] Step 1: Accurately weigh water, disodium EDTA, sodium laureth sulfate, ammonium lauryl sulfate, sodium methyl taurate, glycerin, 1,3-butylene glycol, sodium cocoyl glycinate, cocamidopropyl betaine, polyquaternium-7, APG2000, sodium benzoate, citric acid, and WSK in an emulsifier and heat with stirring until completely dissolved at 85°C. Keep warm for 30 minutes, then cool.

[0144] Step 2: Stir and cool. When the temperature drops to 45°C, add sodium chloride and stir evenly. The stirring speed is 20r / min.

[0145] Step 3: Stir and cool. When the temperature drops to 40°C, add phenoxyethanol, niacinamide, camellia extract, panthenol, arbutin, oligofructose, ginseng enzyme, the combination, horse chestnut extract, olive extract, golden chamomile (Christmas chamomile) extract, macadamia (Macadamia ternifolia) seed oil, avocado (Butyrospermum parkii) butter, ceramide, and fragrance and mix thoroughly. Stir at 20 r / min. Step 4: Filter the product to obtain the shower gel.

[0146] The compositions of Examples 1 to 3 and Comparative Examples 1 to 11 were respectively prepared according to the above method to obtain shower gels of different embodiments or comparative examples, which were respectively named as: Example 1-shower gel, Example 2-shower gel, Example 3-shower gel, Comparative Example 1-shower gel, Comparative Example 2-shower gel, Comparative Example 3-shower gel, Comparative Example 4-shower gel, Comparative Example 5-shower gel, Comparative Example 6-shower gel, Comparative Example 7-shower gel, Comparative Example 8-shower gel, Comparative Example 9-shower gel, Comparative Example 10-shower gel and Comparative Example 11-shower gel.

[0147] Test Example 1: Investigation of the Hair Repair and Nourishing Efficacy of Hair Mask

[0148] 1. Test purpose and principle

[0149] (1) Combing

[0150] Repair means helping to maintain the application area in a normal state. Products with hair repair effects should demonstrate this in three aspects. The hair repair principles are to improve the combability of hair, enhance the smoothness and suppleness of hair, or provide hair with excellent tensile strength. If any one of the indicators of combability, friction, and stretchability is effective, the sample can be considered to have hair repair effects. This test reflects the hair repair effect by evaluating the combability of hair. Factors that affect the combability of individual hair include the friction coefficient between hair fibers and between hair fibers and combs, hair diameter, hair rigidity, and the presence of static charges. Hair products can make hair easier to comb by changing factors such as the friction coefficient, oiliness, and adhesion of hair. This method uses equipment to measure the combability of hair pieces before and after using the sample, and the degree of reduction in combability is used to measure the effectiveness of the sample in improving combability.

[0151] This test method is an in vitro real hair method and is suitable for testing the repair efficacy of rinse-off and leave-in hair products.

[0152] (2) Glossiness

[0153] The various lipids and other substances in hair play a major role in its physical properties, influencing various aspects of its properties, including strength, gloss, smoothness, feel, and suppleness. Therefore, improvements in hair gloss and friction or combability can be used to reflect a product's effectiveness in nourishing hair. This test measures hair nourishment by evaluating hair gloss and combability.

[0154] Light is reflected from the front and back surfaces of hair. As it passes through the hair, it is affected by melanin and tiny pores, resulting in varying degrees of absorption or scattering, resulting in varying degrees of gloss. The degree of hair damage, the cleanliness of the hair and scalp, and the distribution of surface debris are all important factors influencing hair gloss. This test evaluates the glossiness of a sample by observing the change in hair gloss before and after use.

[0155] Factors that affect the combability of individual hair include the friction coefficient between hair fibers and between the hair fiber and the comb, hair diameter, hair rigidity, and the presence of static charge. Hair products can improve combability by modifying factors such as the hair's coefficient of friction, oiliness, and adhesiveness. This method uses a device to measure the combability of a hairpiece before and after application of a sample. The degree of reduction in combability is used to measure the effectiveness of the sample in improving combability.

[0156] This test method is an in vitro method and is suitable for evaluating the effectiveness of hair products that claim to nourish hair, improve hair quality, or similar functions. Hair products include but are not limited to shampoo, shampoo cream, conditioner, hair treatment cream, hair oil, hair lotion, etc.

[0157] Products that act on hair on other parts of the human body and claim hair nourishing effects can refer to this test method.

[0158] 2. Test indicators and judgment criteria

[0159] The test indicators and judgment criteria are shown in Table 8.

[0160] Table 8: Test indicators and judgment criteria

[0161]

[0162] 3. Test instruments

[0163] Main instruments:

[0164] Hair combing instrument, model: XJ810-100N, manufacturer: Shanghai Xiangjie Instrument Technology Co., Ltd. Gloss meter, model: WGG60, manufacturer: Hangzhou Qiwei Instrument Co., Ltd.

[0165] 4 Test methods

[0166] Experimental design: self-controlled before and after

[0167] 4.1 Select eight tresses of human in-vitro hair from the same batch, identical in appearance, and trimmed to the same specifications. Soak the selected tresses in a 5g / L sodium lauryl sulfate solution, place them in a (40.0±1.0)°C constant temperature water bath, rinse thoroughly with clean water, and equilibrate them in a constant temperature and humidity chamber for at least 4 hours.

[0168] 4.2 Measure the glossiness of each hair strand using a gloss meter. The number of tests will depend on the actual situation. Measure the combability of each hair strand using a combing instrument. Repeat the test three times for each strand. Take the average of these values as the initial value for that strand. Discard strands with significantly deviating initial values. Ultimately, ensure at least five valid initial gloss values (before sample C) and five valid initial combability values (before sample A).

[0169] 4.3 Apply or spray the test sample evenly and quantitatively onto the hair tresses according to the instructions. Treat the hair tresses according to the sample instructions. Equilibrate the treated hair tresses in a constant temperature and humidity chamber for at least 4 hours. Measure the glossiness (C) and combability (A) of the hair tresses after application.

[0170] 4.4 Use IBM SPSS statistical analysis software to perform statistical analysis on the data.

[0171] Calculate the combing force change rate K according to the following formula A :

[0172] K A =(A 用样后 / A 用样前 -1)×100%,

[0173] K A The results are expressed as means.

[0174] Calculate the combing force change rate K according to the following formula C :

[0175] K C =(C 用样后 / C 用样前 -1)×100%,

[0176] K C The results are expressed as means.

[0177] 5. Test results

[0178] The test results are shown in Table 9.

[0179] Table 9: Combing force change rate K A and glossiness change rate K C

[0180]

[0181] 6. Results Analysis

[0182] (1) Hair masks comprising the composition provided by the present invention containing hydrolyzed ginsenosides, micrococcal lysate, acetyl glucosamine, Tremella polysaccharide and Asian birch sap (Example 1 - Hair Mask, Example 2 - Hair Mask, Example 3 - Hair Mask) have excellent hair repair and nourishing effects.

[0183] (2) The hair mask provided by the present invention, which contains a composition of hydrolyzed ginsenosides, micrococcal lysate, acetyl glucosamine, tremella polysaccharide and Asian birch sap, synergistically improves the hair repair and nourishing effects of hydrolyzed ginsenosides, micrococcal lysate, acetyl glucosamine, tremella polysaccharide and Asian birch sap, has an unexpected synergistic effect.

[0184] Test Example 2: Investigation of the Soothing Effect of Bath Wash

[0185] 1. Test purpose and principle

[0186] Current research suggests that the occurrence of sensitive skin is a complex process involving skin barrier, neurovascular system, and immune inflammation.

[0187] Hyaluronic acid is the component with the highest content and largest proportion in the extracellular matrix. It can maintain the volume of the extracellular matrix, regulate the secretion of cell growth factors and cytokines, and affect cell adhesion, growth, proliferation and differentiation. Therefore, it plays an important role in maintaining skin moisture and elasticity, wound healing and angiogenesis.

[0188] Hyaluronidase is an endogenous hexosamine enzyme that causes the decomposition of large molecular hyaluronic acid. It is strongly correlated with inflammation and allergies. Studies have reported that various drugs that release histamine from mast cells can regulate the activity of hyaluronidase. Some anti-allergic drugs have strong inhibitory effects on hyaluronidase activity. Therefore, inhibition of hyaluronidase activity is used as an indicator for studying anti-allergic effects.

[0189] Hyaluronidase decomposes macromolecular hyaluronic acid into N-acetylglucosamine. Measuring the N-acetylglucosamine content in the reaction system indirectly reflects hyaluronidase activity. Inhibiting hyaluronidase activity ensures normal hyaluronic acid content and function. Therefore, the soothing effect of a test sample can be determined by the hyaluronidase activity inhibition rate. A higher hyaluronidase activity inhibition rate indicates a stronger soothing effect, while a lower inhibition rate indicates a weaker soothing effect.

[0190] 2. Test indicators

[0191] The test indicators and judgment criteria are shown in Table 10.

[0192] Table 10: Test indicators and judgment criteria

[0193] 3. Test methods

[0194] The Elson-Morgan modified method was used for determination, and the specific operation was as follows:

[0195] a: Sample treatment for this test: The sample (Example 2 - shower gel) was dispersed and diluted with water to form a 5% aqueous solution;

[0196] b: Add 0.5 mL of sample solution and 0.5 mL of 500 U / mL hyaluronidase to test tubes 1 and 2, and 0.5 mL of distilled water and 0.5 mL of pH 5.4 buffer to test tubes 3 and 4, and keep them at 37°C for 20 min; add 0.1 mL of 2.5 mol / L calcium chloride solution, and keep them at 37°C for 20 min; add 0.5 mL of 0.5 mg / mL potassium hyaluronate to test tubes 1 and 3, and add 0.5 mL of pH 5.4 buffer to test tubes 2 and 4, and keep them at 37°C for 40 min; let them stand at room temperature for 10 min, then add 0.5 mL of acetylacetone solution (3.5 mL of acetylacetone dissolved in 50 mL 0.1 mol / L sodium carbonate solution), 0.1 mL NaOH solution (5 mol / L) and 0.5 mL distilled water were placed in a boiling water bath for 15 min; cooled for 5 min, placed in an ice water bath for 10 min; left at room temperature for 10 min, and 1 mL P-DAB color developer (0.8 g DBMA (dimethylaminobenzaldehyde) mixed with 15 mL concentrated HCl and anhydrous ethanol) was added; after thorough shaking, 3.5 mL anhydrous ethanol was added and left at room temperature for 30 min to develop color;

[0197] c: absorbance at 530 nm;

[0198] d: Calculate the hyaluronidase activity inhibition rate using the following formula:

[0199] The calculation formula of hyaluronidase activity inhibition rate:

[0200] Hyaluronidase activity inhibition rate (%) = [1-(A1-A2) / (A3-A4))] × 100%

[0201] Where: A1—absorbance of test tube No. 1;

[0202] A2—is the absorbance of test tube No. 2;

[0203] A3—absorbance of test tube No. 3;

[0204] A4—is the absorbance of test tube No. 4.

[0205] 4. Test results

[0206] The test results are shown in Table 11.

[0207] Table 11: Hyaluronidase activity inhibition rate

[0208]

[0209]

[0210] Conclusion: When the shower gel provided by the present invention is diluted into a 5% aqueous solution, the hyaluronidase activity inhibition rate is 50.00%, while the hyaluronidase activity inhibition rate of the positive control is 8.70%. The hyaluronidase activity inhibition rate of the shower gel containing the composition provided by the present invention is higher than that of the positive control sample, indicating that the shower gel containing the composition provided by the present invention has excellent soothing effect.

[0211] Test Example 3: Moisturizing effect of shampoo

[0212] 1. Test purpose and principle

[0213] Water is indispensable for the formation of the skin's stratum corneum. When the air humidity decreases, the skin's stratum corneum cannot adjust sufficient moisturizing factors in time, the activity of the oil glands decreases, the oil and moisture on the face decreases, and the skin becomes tight and flaky. Applying skin care products containing moisturizing factors externally is the main way to keep the skin hydrated.

[0214] Products with strong hygroscopic properties have strong water-binding abilities, absorbing and retaining a large amount of water. Good sealing properties also minimize water loss. This test method continuously monitors the weight change of the test sample before and after placement to calculate the moisturizing effect of the moisturizing ingredients. This method then plots a moisture retention curve for the test sample over time. The moisture retention curve trends indicate the moisturizing capacity of the test sample and, in turn, evaluates its moisturizing efficacy on the skin.

[0215] 2. Test indicators

[0216] Test indicators and judgment criteria are shown in Table 12.

[0217] Table 12: Test indicators and judgment criteria

[0218]

[0219] 3. Test methods

[0220] (1) Weigh the mass (Mo) of the glass plate with 3 cm of adhesive tape on an analytical balance. Use a glass rod to evenly spread the sample on the glass plate with 3 cm of adhesive tape. Weigh the mass (M1) on an analytical balance. Then place the sample in a desiccator at the required constant humidity (the constant temperature and humidity in this test are: 25.1°C, 50%).

[0221] (2) Regularly weigh the sample glass plate to be tested, weigh the mass (M2), test its moisture retention rate, and immediately place it in a desiccator at a constant humidity after weighing to draw a moisture retention rate curve;

[0222] Moisturizing rate calculation formula:

[0223] Moisture retention rate = (M2-M0) / (M1-M0)×100%

[0224] Where: M0 is the mass of the empty plate (g);

[0225] M1 is the mass of the glass plate after sample addition (g);

[0226] M2 is the mass (g) after being placed in the desiccator for several hours.

[0227] 4. Test results

[0228] The test results are shown in Table 13 and Figure 1 .

[0229] Table 13: Moisture Retention Test Results

[0230]

[0231] in conclusion:

[0232] Depend on Figure 1 As can be seen from the results in Table 13, the moisturizing rate broken line of the shampoo containing the composition provided by the present invention is above the positive control, that is, under the conditions of 25.1°C and 50% RH, the moisturizing effect of the shampoo containing the composition provided by the present invention is better than that of the 5% glycerol positive control, and the shampoo containing the composition provided by the present invention has an excellent moisturizing effect.

[0233] Test Example 4: Oil Control Effect of Shampoo

[0234] 1. Test purpose and principle

[0235] Seborrhea is a disorder of sebaceous gland function, resulting in excessive sebum secretion. This leads to excess oil accumulation in the skin and hair, resulting in a greasy, shiny appearance and the appearance of scaling. Sex hormone imbalance, primarily elevated androgen levels, can lead to increased sebaceous gland secretion. Androgens, androgen receptors, and the activity of androgen-metabolizing enzymes can all affect oil secretion.

[0236] There are two types of the androgen-metabolizing enzyme 5α-reductase: type I is found in the liver and sebaceous glands, while type II is primarily found in gonadal tissue and hair follicles in the scalp and beard. 5α-reductase converts the androgen testosterone and the reduced coenzyme (NADPH) into dihydrotestosterone. Dihydrotestosterone's binding activity to the androgen receptor is five times that of testosterone, promoting cellular processes and inducing increased sebum secretion in the sebaceous glands. 5α-reductase activity can influence oil secretion in different parts of the body. Based on this principle, this experiment used UV spectrophotometry to determine the concentration of the reduced coenzyme, using changes in the reduced coenzyme concentration as a proxy for 5α-reductase activity. Finally, the degree of inhibition of 5α-reductase activity by the experimental sample was used to preliminarily assess the oil-control efficacy of the cosmetic product. A higher inhibition rate indicates a stronger oil-control effect, while a lower inhibition rate indicates a weaker oil-control effect.

[0237] 2. Test indicators

[0238] Test indicators and judgment criteria are shown in Table 14.

[0239] Table 14: Test indicators and judgment criteria

[0240] Test indicators Judgment criteria Oil control effect The higher the 5α-reductase activity inhibition rate, the stronger the oil control effect of the substance.

[0241] 3. Test methods

[0242] a. Preparation of Tris buffer: Accurately weigh 607 mg of Tris, 29 mg of EDTA-Na, 610 mg of MgCl2·6H2O, 1.46 g of NaCl, and 22.5 g of sucrose. Dissolve in 20 μl of dimercaptoethanol and dilute to 500 ml with distilled water. Adjust the pH to 6.0 with hydrochloric acid and store at 4°C until ready for use.

[0243] b: Preparation of steroid 5α-reductase solution: SD male rats, weighing approximately 300 g, were sacrificed by cervical dislocation. The testicular and epididymal tissues were collected and immersed in 50 ml of 4°C Tris buffer. The tissues were minced and homogenized using a homogenizer at 0°C. The supernatant was filtered through a single layer of gauze and centrifuged at 4°C (10,000 rpm, 5 min) to obtain a suspension with an upper layer of fat. The upper layer of fat was discarded, and the lower layer containing 5α-reductase was divided into centrifuge tubes to obtain the steroid 5α-reductase solution, which was stored in a -70°C refrigerator until use.

[0244] c: Preparation of standard solution: Accurately weigh 28.84 mg of testosterone, dissolve it in anhydrous ethanol and dilute to 100 ml to obtain testosterone stock solution (1 mmol / L); accurately weigh 25 mg of NADPH-Na4, completely dissolve it in 1000 μl of distilled water, aspirate 835 μl, dilute to 25 ml with Tris buffer to obtain NADPH stock solution (1 mmol / L), and store it in a refrigerator for later use.

[0245] d: Sample treatment: Weigh 2 g of sample, add Tris buffer to 10 ml, shake well to disperse, ultrasonicate for 10 min, centrifuge at 5000 rpm for 5 min, and take the supernatant for experiment.

[0246] e: Preparation of positive control solution: Dilute finasteride to 2.5 mg / mL with Tris buffer to obtain a positive control solution; f: Determination of 5α-reductase inhibitory activity of samples: Mix 20 μL of the test sample or positive control solution, 2 mL of distilled water, 20 μL of testosterone stock solution, 600 μL of steroid 5α-reductase solution, and 200 μL of NADPH stock solution, and then react at 37°C.

[0247] g: absorbance at 340 nm at 0 min and 60 min of reaction;

[0248] h: Calculation of 5α-reductase activity inhibition rate;

[0249] 5α-reductase activity inhibition rate calculation formula:

[0250] 5α-reductase activity inhibition rate (%) = (ΔA1-ΔA2) / ΔA1×100%

[0251] Where:

[0252] ΔA1-negative experiment: the difference between the absorbance at 0 min and the absorbance at 60 min;

[0253] ΔA2 - the difference between the absorbance at 0 min and the absorbance at 60 min of the sample test.

[0254] 4. Test results

[0255] The test results are shown in Table 15.

[0256] Table 15: 5α-reductase activity inhibition rate test results

[0257] sample 5α-reductase activity inhibition rate Example 2 - Shower Gel 36.00％ Positive control (2.5 mg / mL finasteride) 34.67％

[0258] in conclusion:

[0259] The shampoo containing the composition provided by the present invention has an inhibition rate of 36.00% on the activity of 5α-reductase. According to the above test results, the shampoo containing the composition provided by the present invention has excellent oil control effect.

[0260] Test Example 5: Investigation of the Anti-dandruff Effect of Shampoo

[0261] 1. Test purpose and principle

[0262] Recent studies have found that the massive reproduction of Malassezia furfur on the scalp can cause excessive proliferation of the scalp's stratum corneum, thereby prompting the abnormal shedding of stratum corneum cells in the form of white or gray scales. This scale is dandruff. The massive reproduction of Malassezia furfur is one of the important factors causing dandruff.

[0263] This study conducted an inhibition ring test to observe the in vitro antibacterial activity of anti-dandruff shampoos against Malassezia, a common fungus on the surface of seborrheic dermatitis lesions, in order to indirectly evaluate the anti-dandruff efficacy of hair products.

[0264] 2. Test indicators

[0265] Test indicators and judgment criteria are shown in Table 16.

[0266] Table 16: Test indicators and judgment criteria

[0267]

[0268] 3. Test methods

[0269] a: Take 20 μl of sample solution and drop it on a 5 mm filter paper to prepare a test piece;

[0270] b: Use 5×10 5 ~5×10 6 A suspension of Malassezia furfur (ATCC 44344) at cfu / mL was evenly spread on the surface of a Leeming & Notman medium plate to prepare a contaminated plate.

[0271] c. Place the test piece on the contaminated plate and place in a 30°C incubator. Incubate for 5-7 days and observe the results. Use a vernier caliper to measure the diameter of the inhibition ring and record it. Repeat the test 4 times.

[0272] 4. Test results

[0273] The test results are shown in Table 17.

[0274] Table 17: 5α-reductase activity inhibition rate test results

[0275]

[0276] Note: Example 2 - Shampoo concentration: stock solution.

[0277] Conclusion: The shampoo containing the composition provided by the present invention has an inhibition zone diameter of more than 7 mm against Malassezia furfur (ATCC 44344) after 7 days of action, indicating that the shampoo containing the composition provided by the present invention has excellent anti-dandruff efficacy.

[0278] Test Example 6: Investigation of the Mildness of Shampoo

[0279] 1. Test purpose and principle

[0280] The chorioallantoic membrane (CAM) is a respiratory membrane that surrounds the chick embryo. This study utilizes the intact, clear, and transparent vascular system of the chorioallantoic membrane in mid-stage incubation. A certain amount of test substance is directly exposed to the chorioallantoic membrane. After a period of exposure, changes in toxicity indicators (such as bleeding, coagulation, and vascular melting) are observed. These indicators reflect changes in the morphology, structure, color, and permeability of the blood vessels and vascular network, as well as phenomena such as chorioallantoic membrane protein denaturation and the degree of damage.

[0281] This experiment involves placing a certain amount of the test substance in direct contact with the chorioallantoic membrane of a chick embryo. After a specified period of exposure, changes in the chick embryo chorioallantoic membrane are observed and scored. The irritation of the sample to the CAM is observed to indirectly assess the sample's mild (non-irritating) efficacy.

[0282] 2. Test indicators

[0283] Test indicators and judgment criteria are shown in Table 18.

[0284] Table 18: Test indicators and judgment criteria

[0285] Test indicators Judgment criteria Mild effect When the irritation classification of the test product is non-irritating, the product is judged to be mild and non-irritating

[0286] 3. Test methods

[0287] a: Chorioallantoic membrane (CAM) was prepared from 9-day-old chicken embryos (chicken embryo strain: Laihang chicken);

[0288] b: Prepare the test substance (Example 2 - shampoo) and the control substance (the results of the negative control substance, positive control substance, and reference substance control test in this test are all acceptable);

[0289] c) Endpoint evaluation method: Apply 0.3 mL of the test substance directly to the CAM. After 3 minutes, gently rinse the CAM with normal saline. After 30 seconds, observe the extent of each toxic effect. Observe and record the time of onset of bleeding, coagulation, and vascular lysis on the CAM, as well as the extent of the toxic effect, and calculate a score.

[0290] 4. Test results

[0291] The test results are shown in Table 19.

[0292] Table 19: Test results

[0293]

[0294]

[0295] Bleeding: no bleeding (0 points), mild bleeding (1 point), moderate bleeding (2 points), severe bleeding (3 points);

[0296] Coagulation: no coagulation (0 points), mild coagulation (1 point), moderate coagulation (2 points), severe coagulation (3 points);

[0297] Vascular ablation: no vascular ablation (0 points), mild vascular ablation (1 point), moderate vascular ablation (2 points), severe vascular ablation (3 points).

[0298]

[0299] Table 20: End point scoring results evaluation

[0300] End point score Irritation classification ES≤12 No / mild irritation 12<ES<16 Moderate irritation ES≥16 Strong irritation

[0301] Final calculation result: ES = 7.00, the result is evaluated as no / mild irritation

[0302] in conclusion

[0303] The shampoo containing the composition provided by the present invention was tested using the endpoint evaluation method, and the endpoint score ES=7.00 was evaluated as non-irritating / mildly irritating, indicating that the shampoo containing the composition provided by the present invention is mild and non-irritating.

[0304] Test Example 7: Investigation of the soothing effect of shampoo

[0305] The test was carried out according to the method of “Test Example 2: Investigation of the Soothing Efficacy of Bath Liquid”. The results are shown in Table 21.

[0306] Table 21: Hyaluronidase activity inhibition rate

[0307] sample Hyaluronidase activity inhibition rate Example 1 - Shampoo 44.17％ Example 2 - Shampoo 46.74％ Example 3 - Shampoo 49.65％ Comparative Example 6-Shampoo 7.07％ Comparative Example 7-Shampoo 12.90％ Comparative Example 8-Shampoo 11.72％ Comparative Example 9-Shampoo 10.04％ Comparative Example 10-Shampoo 16.04％ Comparative Example 11-Shampoo 5.07％ Positive control (2 mg / mL isoliquiritigenin solution) 8.70％

[0308] in conclusion:

[0309] (1) When the shampoo containing the composition provided by the present invention is diluted into a 5% aqueous solution, the hyaluronidase activity inhibition rate is 46.74%, while the hyaluronidase activity inhibition rate of the positive control is 8.70%. The hyaluronidase activity inhibition rate of the shampoo containing the composition provided by the present invention is higher than that of the positive control sample, indicating that the shampoo containing the composition provided by the present invention has excellent soothing effect.

[0310] (2) The shampoo provided by the present invention, which contains a composition of hydrolyzed ginsenosides, micrococcal lysate, acetyl glucosamine, tremella polysaccharide and Asian birch sap, synergistically improves the soothing effect, and has an unexpected synergistic effect. The method of the present invention has been described through the preferred embodiments. It is obvious that relevant personnel can modify or appropriately change and combine the methods and applications described herein within the content, spirit and scope of the present invention to realize and apply the technology of the present invention. Those skilled in the art can refer to the content of this article and appropriately improve the process parameters to achieve it. It should be pointed out in particular that all similar replacements and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention.

Claims

1. A composition, characterized in that The invention comprises active ingredients, which include hydrolyzed ginsenosides, micrococcal lysate, acetyl glucosamine, Asian birch sap and tremella polysaccharide.

2. The composition according to claim 1, wherein the mass ratio of the hydrolyzed ginsenosides, micrococcal lysate, acetyl glucosamine, Asian birch sap and Tremella fuciformis polysaccharide is (0.01-0.03):(3-5.1):(0.5-1.5):(70-88):(0.05-0.15) or (0.01-0.03):(3-5.0025):(0.5-1.5):(70.81625-87.60375):(0.05-0.15), or 0.01:3:0.5:87.60375:0.05, or 0.02:3.75:1:79.4575:0.1, or 0.03:5.0025:1.5:70.81625:0.15; and / or The composition further comprises a solvent; and / or The solvent comprises at least one of 1,3-butanediol, 1,2-hexanediol and water; and / or The solvent includes 1,3-butanediol, 1,2-hexanediol and water; and / or The composition further comprises other ingredients; and / or The other ingredients include: At least one of C12-13 pareth-9, 1,2-pentanediol and p-hydroxyacetophenone.

3. The composition according to any one of claims 1 to 2, wherein the content of the hydrolyzed ginsenosides is 0.01 wt% to 0.03 wt% or 0.02 wt% based on the total mass of the composition; and / or Calculated based on the total mass of the composition, the content of the micrococcal lysate is 3.0wt%-5.1wt%, 3.0wt%-5.0025wt% or 3.75wt%; and / or Calculated based on the total mass of the composition, the content of the acetyl glucosamine is 0.5wt%-1.5wt% or 1.0wt%; and / or Based on the total mass of the composition, the content of the Asian birch sap is 70wt%-90wt%, or 70wt%-87.7wt%, or 70.8wt%, 79.5wt% or 87.6wt%, or 70.81625wt%, 79.4575wt% or 87.60375wt%; and / or Calculated based on the total mass of the composition, the content of Tremella polysaccharide is 0.05wt%-0.15wt% or 0.1wt%; and / or Based on the total mass of the composition, the content of the solvent is 8.0 wt% to 27.6 wt% or 8.4 wt% to 22.0 wt% or 8.4275 wt%, 15.155 wt% or 21.875 wt%; and / or Calculated based on the total mass of the composition, the content of 1,3-butanediol is 3wt%-7wt%, or 3wt%-6.9925wt%, or 5wt%; and / or Calculated based on the total mass of the composition, the content of 1,2-hexanediol is 0.4wt%-0.6wt% or 0.5wt%; and / or Calculated based on the total mass of the composition, the content of 1,2-pentanediol is 0 wt%-0.015 wt% or 0.00375 wt%, 0.0075 wt% or 0.01125 wt%; and / or Calculated based on the total mass of the composition, the water content is 4wt%-20wt% or 4wt%-15wt% or 4.8275wt%, 9.655wt%, 14.4825wt%; and / or Calculated based on the total mass of the composition, the content of p-hydroxyacetophenone is 0.4wt%-0.6wt% or 0.5wt%; and / or Calculated based on the total mass of the composition, the content of the C12-13 polyether-9 is 0.005 wt%-0.015 wt% or 0.01 wt%.

4. The composition according to any one of claims 1 to 3, wherein, calculated based on the total mass of the composition, the content of the hydrolyzed ginsenosides is 0.01wt%-0.03wt%, the content of the micrococcal lysate is 3.0wt%-5.1wt%, the content of acetyl glucosamine is 0.5wt%-1.5wt%, the content of the Asian birch sap is 70wt%-87.7wt%, the content of the Tremella fuciformis polysaccharide is 0.05wt%-0.15wt%, the content of the solvent is 8.4wt%-22.0wt%, the content of p-hydroxyacetophenone is 0.4wt%-0.6wt%, and the content of C12-13 pareth-9 is 0.005wt%-0.015wt%; or Calculated based on the total mass of the composition, the content of the hydrolyzed ginsenosides is 0.01wt%-0.03wt%, the content of the micrococcal lysate is 3.0wt%-5.0025wt%, the content of the acetyl glucosamine is 0.5wt%-1.5wt%, the content of the Asian birch sap is 70.81625wt%-87.60375wt%, the content of the tremella polysaccharide is 0.05wt%-0.15wt%, the content of the p-hydroxyacetophenone is 0.4wt%-0.6wt%, the content of the C12-13 paraether-9 is 0.005wt%-0.015wt%, and the content of the solvent is 8.4275wt%-21.875wt%; or Calculated based on the total mass of the composition, the content of the hydrolyzed ginsenosides is 0.01wt%-0.03wt%, the content of the micrococcal lysate is 3.0wt%-5.0025wt%, the content of the acetyl glucosamine is 0.5wt%-1.5wt%, the content of the tremella polysaccharide is 0.05wt%-0.15wt%, the content of the p-hydroxyacetophenone is 0.4wt%-0.6wt%, the content of the C12-13 paraether-9 is 0.005wt%-0.015wt%, the content of the solvent is 8.4275wt%-21.875wt%, and the balance is the Asian birch sap; or Calculated based on the total mass of the composition, the content of the hydrolyzed ginsenosides is 0.01wt%-0.03wt%, the content of the micrococcal lysate is 3.0wt%-5.0025wt%, the content of the acetyl glucosamine is 0.5wt%-1.5wt%, the content of the tremella polysaccharide is 0.05wt%-0.15wt%, the content of the p-hydroxyacetophenone is 0.4wt%-0.6wt%, and the C12-13 chain is 0. The content of pareth-9 is 0.005wt%-0.015wt%, the content of 1,3-butanediol is 3wt%-6.9925wt%, the content of 1,2-hexanediol is 0.4wt%-0.6wt%, the content of 1,2-pentanediol is 0.00375wt%-0.01125wt%, the content of water is 4.8275wt%-14.4825wt%, and the balance is the Asian birch sap; or Calculated based on the total mass of the composition, the content of the hydrolyzed ginsenosides is 0.01wt%, the content of the micrococcal lysate is 3.0wt%, the content of the acetyl glucosamine is 0.5wt%, the content of the tremella polysaccharide is 0.05wt%, the content of the p-hydroxyacetophenone is 0.4wt%, the content of the C12-13 paraether-9 is 0.005wt%, the content of the 1,3-butanediol is 3wt%, the content of the 1,2-hexanediol is 0.6wt%, the content of the 1,2-pentanediol is 0.00375wt%, the content of the water is 4.8275wt%, and the balance is the Asian birch sap; or Calculated based on the total mass of the composition, the content of the hydrolyzed ginsenosides is 0.02wt%, the content of the micrococcal lysate is 3.75wt%, the content of the acetyl glucosamine is 1wt%, the content of the tremella polysaccharide is 0.1wt%, the content of the p-hydroxyacetophenone is 0.5wt%, the content of the C12-13 paraether-9 is 0.01wt%, the content of the 1,3-butanediol is 5wt%, the content of the 1,2-hexanediol is 0.5wt%, the content of the 1,2-pentanediol is 0.0075wt%, the content of the water is 9.655wt%, and the balance is the Asian birch sap; or Calculated based on the total mass of the composition, the content of the hydrolyzed ginsenosides is 0.03wt%, the content of the micrococcal lysate is 5.0025wt%, the content of the acetyl glucosamine is 1.5wt%, the content of the tremella polysaccharide is 0.15wt%, the content of the p-hydroxyacetophenone is 0.6wt%, the content of the C12-13 paraether-9 is 0.015wt%, the content of the 1,3-butanediol is 6.9925wt%, the content of the 1,2-hexanediol is 0.4wt%, the content of the 1,2-pentanediol is 0.01125wt%, the content of the water is 14.4825wt%, and the balance is the Asian birch sap.

5. The composition according to any one of claims 1 to 4, characterized in that The composition comprises rare ginsenoside PLF, ASM cytokinin, acetyl glucosamine, fermented Tremella polysaccharide Trepoly-01 and Asian white birch (BETULAPLATYPHYLLA) sap, and optionally at least one selected from 1,3-butanediol, 1,2-hexanediol and p-hydroxyacetophenone; The rare ginsenoside PLF is composed of hydrolyzed ginsenosides, water, C12-13 parether-9, 1,3-butanediol, 1,2-pentanediol and 1,2-hexanediol, wherein the content of the hydrolyzed ginsenosides in the rare ginsenoside PLF is 3wt%-5wt%, the content of the C12-13 parether-9 in the rare ginsenoside PLF is 1.8wt%-2.2wt%, the content of the 1,3-butanediol in the rare ginsenoside PLF is 9wt%-11wt%, the content of the 1,2-pentanediol in the rare ginsenoside PLF is 1.3wt%-1.7wt%, the content of the 1,2-hexanediol in the rare ginsenoside PLF is 1.3wt%-1.7wt%, and the content of water in the rare ginsenoside PLF is 79wt%-83wt%; The ASM cytokinin is composed of micrococcal lysate and 1,3-butanediol, wherein the content of the micrococcal lysate in the ASM cytokinin is 70wt%-78wt%, and the balance is 1,3-butanediol; The fermented tremella polysaccharide Trepoly-01 is composed of tremella polysaccharide, 1,3-butanediol, 1,2-hexanediol, p-hydroxyacetophenone and water, wherein the content of the tremella polysaccharide in the fermented tremella polysaccharide is 0.9wt%-1.1wt%, the content of the 1,3-butanediol in the fermented tremella polysaccharide is 2wt%-8wt%, the content of the 1,2-hexanediol in the fermented tremella polysaccharide is 0.5wt%-1wt%, the content of the p-hydroxyacetophenone in the fermented tremella polysaccharide is 0.2wt%-0.8wt%, and the content of the water in the fermented tremella polysaccharide is 89.1wt%-96.4wt%; Preferably, the rare ginsenoside PLF is composed of hydrolyzed ginsenosides, water, C12-13 pareth-9, 1,3-butanediol, 1,2-pentanediol and 1,2-hexanediol, wherein the content of the hydrolyzed ginsenosides in the rare ginsenoside PLF is 4wt%, the content of the C12-13 pareth-9 in the rare ginsenoside PLF is 2wt%, the content of the 1,3-butanediol in the rare ginsenoside PLF is 10wt%, the content of the 1,2-pentanediol in the rare ginsenoside PLF is 1.5wt%, the content of the 1,2-hexanediol in the rare ginsenoside PLF is 1.5wt%, and the content of water in the rare ginsenoside PLF is 81wt%; Preferably, the ASM cytokinin is composed of micrococcal lysate and 1,3-butanediol, wherein the content of the micrococcal lysate in the ASM cytokinin is 75 wt %, and the balance is 1,3-butanediol; Preferably, the fermented tremella polysaccharide Trepoly-01 is composed of tremella polysaccharide, 1,3-butanediol, 1,2-hexanediol, p-hydroxyacetophenone and water, wherein the content of the tremella polysaccharide in the fermented tremella polysaccharide is 1wt%, the content of the 1,3-butanediol in the fermented tremella polysaccharide is 5wt%, the content of the 1,2-hexanediol in the fermented tremella polysaccharide is 1wt%, the content of the p-hydroxyacetophenone in the fermented tremella polysaccharide is 0.5wt%, and the content of the water in the fermented tremella polysaccharide is 92.5wt%.

6. A method for preparing the composition according to any one of claims 1 to 4, characterized in that: The method comprises: mixing the active ingredient and the solvent, or mixing the active ingredient, the solvent and other ingredients, and stirring until the mixture is completely dissolved and transparent to obtain the composition.

7. A method for preparing the composition according to any one of claims 1 to 5, characterized in that: include: Rare ginsenoside PLF, ASM cytokinin, acetyl glucosamine, fermented Tremella fuciformis polysaccharide Trepoly-01, and Asian birch (Betula typhi) tree sap, and optionally at least one selected from 1,3-butanediol, 1,2-hexanediol, and p-hydroxyacetophenone, are mixed and stirred until completely dissolved and transparent to obtain the composition; The rare ginsenoside PLF is composed of hydrolyzed ginsenosides, water, C12-13 parether-9, 1,3-butanediol, 1,2-pentanediol and 1,2-hexanediol, wherein the content of the hydrolyzed ginsenosides in the rare ginsenoside PLF is 3wt%-5wt%, the content of the C12-13 parether-9 in the rare ginsenoside PLF is 1.8wt%-2.2wt%, the content of the 1,3-butanediol in the rare ginsenoside PLF is 9wt%-11wt%, the content of the 1,2-pentanediol in the rare ginsenoside PLF is 1.3wt%-1.7wt%, the content of the 1,2-hexanediol in the rare ginsenoside PLF is 1.3wt%-1.7wt%, and the content of water in the rare ginsenoside PLF is 79wt%-83wt%; The ASM cytokinin is composed of micrococcal lysate and 1,3-butanediol, wherein the content of the micrococcal lysate in the ASM cytokinin is 70wt%-78wt%, and the balance is 1,3-butanediol; The fermented tremella polysaccharide Trepoly-01 is composed of tremella polysaccharide, 1,3-butanediol, 1,2-hexanediol, p-hydroxyacetophenone and water, wherein the content of the tremella polysaccharide in the fermented tremella polysaccharide is 0.9wt%-1.1wt%, the content of the 1,3-butanediol in the fermented tremella polysaccharide is 2wt%-8wt%, the content of the 1,2-hexanediol in the fermented tremella polysaccharide is 0.5wt%-1wt%, the content of the p-hydroxyacetophenone in the fermented tremella polysaccharide is 0.2wt%-0.8wt%, and the content of the water in the fermented tremella polysaccharide is 89.1wt%-96.4wt%. Preferably, the rare ginsenoside PLF is prepared from hydrolyzed ginseng. Saponins, water, C12-13 pareth-9, 1,3-butanediol, 1,2-pentanediol and 1,2-hexanediol are composed, wherein the content of the hydrolyzed ginsenosides in the rare ginsenoside PLF is 4wt%, the content of the C12-13 pareth-9 in the rare ginsenoside PLF is 2wt%, the content of the 1,3-butanediol in the rare ginsenoside PLF is 10wt%, the content of the 1,2-pentanediol in the rare ginsenoside PLF is 1.5wt%, the content of the 1,2-hexanediol in the rare ginsenoside PLF is 1.5wt%, and the content of the water in the rare ginsenoside PLF is 81wt%; Preferably, the ASM cytokinin is composed of micrococcal lysate and 1,3-butanediol, wherein the content of the micrococcal lysate in the ASM cytokinin is 75 wt %, and the balance is 1,3-butanediol; Preferably, the fermented tremella polysaccharide Trepoly-01 is composed of tremella polysaccharide, 1,3-butanediol, 1,2-hexanediol, p-hydroxyacetophenone and water, wherein the content of the tremella polysaccharide in the fermented tremella polysaccharide is 1wt%, the content of the 1,3-butanediol in the fermented tremella polysaccharide is 5wt%, the content of the 1,2-hexanediol in the fermented tremella polysaccharide is 1wt%, the content of the p-hydroxyacetophenone in the fermented tremella polysaccharide is 0.5wt%, and the content of the water in the fermented tremella polysaccharide is 92.5wt%.

8. A cosmetic or cleaning product, characterized in that: A composition comprising the composition according to any one of claims 1 to 5 or the composition prepared by the preparation method according to any one of claims 6 to 7; Optionally, the cosmetics or cleaning products are used for anti-oxidation, anti-inflammation, skin repair, antibacterial and antimicrobial effects, soothing and anti-allergic effects, promoting hyaluronic acid production, exfoliating waste keratin, promoting skin renewal, moisturizing, improving fine lines, repairing hair, nourishing hair, anti-dandruff and / or oil control.

9. Use of the composition according to any one of claims 1 to 5 or the composition prepared by the preparation method according to any one of claims 6 to 7 in the preparation of cosmetics or cleaning products; Optionally, the cosmetics or cleaning products are used for anti-oxidation, anti-inflammation, skin repair, antibacterial and antimicrobial effects, soothing and anti-allergic effects, promoting hyaluronic acid production, exfoliating waste keratin, promoting skin renewal, moisturizing, improving fine lines, repairing hair, nourishing hair, anti-dandruff and / or oil control.

10. The cosmetic or cleaning product according to claim 8 or the use according to claim 9, wherein the cosmetic or cleaning product is selected from the following forms of cosmetics or cleaning products: liquids, creams, hand creams, essences, gels, paste bars, sprays, ointments, liquid detergents, soap bars, shower gels, facial cleansers, shampoos, conditioners, hair care oils, body lotions, hand creams, pastes, foams, powders, mousses, hydrogels, film-forming products, facial masks, and hair masks.

Images