Toothpaste thickening agent based on collagen-bletilla striata polysaccharide-xanthan gum composite system and preparation method thereof

Abstract

The invention provides a toothpaste thickener based on a collagen-bletilla striata polysaccharide-xanthan gum composite system, which is prepared from the following raw materials in percentage by mass: 0.5-5% of bletilla striata polysaccharide, 0.3-3% of xanthan gum, 0.1-2% of collagen and the balance of deionized water, and the sum of the mass percentages of the raw materials is 100%. The invention also provides a preparation method of the toothpaste thickening agent. The toothpaste thickening agent based on the collagen-bletilla striata polysaccharide-xanthan gum composite system, provided by the invention, has relatively good thixotropy, functionality and stability at the same time.

Description

Technical Field

[0001] The invention relates to a toothpaste thickener, in particular to a toothpaste thickener based on a collagen-bletilla striata polysaccharide-xanthan gum composite system and a preparation method thereof. Background Art

[0002] Traditional toothpaste thickening systems mainly rely on synthetic polymer materials such as sodium carboxymethyl cellulose (CMC) and carbomer. Although toothpaste thickeners with CMC as the main thickener can provide a higher viscosity (about 8000mPa·s), 3-5wt% of CMC needs to be added, which causes the toothpaste paste to easily agglomerate due to entanglement of CMC molecular chains during storage. In addition, CMC lacks biological activity and cannot meet consumers' demand for "functional toothpaste". Another type of improvement scheme attempts to use a natural polysaccharide compound, through the pseudoplasticity of xanthan gum and the high viscosity of konjac gum for synergistic thickening, but its thixotropy is insufficient (the thixotropic ring area is only 320Pa·s -1 ), the paste has difficulty quickly recovering its structure after extrusion, affecting the user experience. In addition, the xanthan gum-konjac gum system has poor compatibility with functional ingredients. For example, the addition of hemostatic ingredients can cause the colloidal network to collapse.

[0003] In recent years, some research has attempted to incorporate protein ingredients into oral care products to enhance their repair function. For example, collagen's film-forming properties can protect the oral mucosa. However, this relies on carbomer as a thickener (at a 1.2 wt% addition), which requires strict control of the pH of the system between 5.5 and 6.0. Otherwise, the carbomer gel network is prone to destabilization. More notably, existing formulations of natural polysaccharides and proteins often suffer from a "function-rheology" conflict. For example, patent application number CN201611184116.0 combines chitosan with whey protein for toothpaste. While this toothpaste exhibits some antibacterial and repair effects, the cationic properties of chitosan and the electrostatic interaction of whey protein cause the paste's viscosity to vary dramatically with shear rate, making it prone to dripping from the toothbrush during use.

[0004] In summary, the existing toothpaste thickening system faces three bottlenecks: First, synthetic thickeners are not environmentally friendly and have a single function; Secondly, it is difficult to combine natural ingredients to meet the requirements of thixotropy and high viscosity; Third, the addition of functional active ingredients often comes at the expense of system stability. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to provide a toothpaste thickener based on a collagen-bletilla striata polysaccharide-xanthan gum composite system, which has good thixotropy, functionality and stability.

[0006] In order to solve the above technical problems, the technical solution of the present invention is: A toothpaste thickener based on a collagen-bletilla striata polysaccharide-xanthan gum composite system is prepared from the following raw materials in percentage by mass: 0.5-5% bletilla striata polysaccharide, 0.3-3% xanthan gum, 0.1-2% collagen, and the balance being deionized water, wherein the sum of the percentages by mass of the raw materials is 100%.

[0007] Bletillastriata polysaccharide (BSP), a natural high-molecular-weight polysaccharide extracted from the tubers of the orchid plant Bletilla striata through water extraction and alcohol precipitation, has significant benefits in oral care. It exhibits excellent hemostatic properties by promoting platelet aggregation, activating coagulation factors, and forming a gel-like barrier on the wound surface, rapidly stopping bleeding from oral injuries. This can reduce the amount and duration of bleeding, such as after tooth extraction or oral mucosal trauma. Bletillastriata polysaccharide promotes wound healing by stimulating oral mucosal cell proliferation, regulating extracellular matrix synthesis, and promoting collagen production. It also regulates local inflammatory responses, creating a favorable environment for wound repair, such as oral ulcers. Bletillastriata polysaccharide also possesses antibacterial properties, altering bacterial cell membrane permeability, leading to their death, while also activating the immune system to enhance the clearance of common harmful bacteria, such as Streptococcus mutans, which causes caries, and Porphyromonas gingivalis, which causes periodontitis. In addition, Bletilla striata polysaccharide can form a moist protective film on the surface of the oral mucosa with its good water absorption and water retention properties, preventing water loss, effectively alleviating the dry mouth discomfort of patients with xerostomia, and maintaining the normal physiological function of the oral mucosa.

[0008] Recombinant humanized type III collagen has excellent tissue compatibility, helping to repair oral mucosa and soft tissues and restore oral health. Recombinant humanized type III collagen can tightly bind to the collagen network in damaged oral mucosa, forming a complete reticular fiber structure, thereby providing a microenvironment conducive to the repair and regeneration of oral mucosal cells.

[0009] Furthermore, the ratio between the sum of the mass of the xanthan gum and collagen and the mass of the bletilla striata polysaccharide of the present invention is (0.6-1.2):1.

[0010] Furthermore, the Bletilla striata polysaccharide of the present invention has a molecular weight of 50-200 kDa. It provides shear-thinning thixotropy (thixotropy index > 0.4) through its β-(1→4)-mannoglucan backbone, while also exerting antibacterial effects (MIC against oral Streptococcus = 1.25 mg / mL) through its phenolic hydroxyl groups.

[0011] Furthermore, the xanthan gum described in the present invention is KELTROL® HP from CP Kelco. Xanthan gum is used to construct a "rigid skeleton" network, improve suspension stability, prevent abrasive sedimentation, and can form high-strength crosslinks with hydrogen bonds of Bletilla striata polysaccharide.

[0012] Furthermore, the collagen protein of the present invention is recombinant humanized type III collagen. Recombinant humanized type III collagen can fill the network gap of bletilla striata polysaccharide / xanthan gum through hydrophobic interaction, thereby increasing the zero shear viscosity (η0≥10 4 mPa·s).

[0013] Another technical problem to be solved by the present invention is to provide a method for preparing the toothpaste thickener based on the collagen-bletilla striata polysaccharide-xanthan gum composite system.

[0014] To solve the above technical problems, the technical solution is: A method for preparing a toothpaste thickener based on a collagen-bletilla striata polysaccharide-xanthan gum composite system comprises the following steps: S1. Weigh each ingredient according to mass percentage, mix Bletilla striata polysaccharide, xanthan gum, and half the weight of deionized water, stir at 60-70°C (above the helix-coil transition temperature of xanthan gum, which promotes molecular chain unfolding) for 20-40 minutes, and cool to 35°C to obtain mixed solution A. S2. Dissolve the collagen in the remaining deionized water at 36-40°C (to prevent denaturation of the collagen), adjust the pH to 6.5-7.0 (to avoid precipitation at the isoelectric point of the collagen), and obtain a collagen solution. S3. Add the collagen solution obtained in step S2 to the mixed solution A obtained in step S1 to obtain a mixed solution B. Homogenize the mixed solution B three times and then vacuum degas for 10-20 minutes to obtain a toothpaste thickener based on a collagen-bletilla striata polysaccharide-xanthan gum composite system. Furthermore, in step S1 of the present invention, the stirring speed is 300-500 rpm.

[0015] Furthermore, in step S2 of the present invention, a citric acid / sodium citrate buffer system prepared by mixing citric acid and sodium citrate in a mass ratio of 2:3 is used to adjust the pH value.

[0016] Furthermore, in step S3 of the present invention, the homogenization pressure is 20-40 MPa, and the vacuum degassing pressure is -0.08 MPa.

[0017] Compared with the prior art, the present invention has the following beneficial effects: (1) The present invention achieves a balance of "shear thinning-network enhancement-functional activity" in a single system through the compounding of collagen, bletilla striata polysaccharide and xanthan gum and the synergistic effect of the three elements, thus solving the inherent contradiction between thixotropy, functionality and stability of traditional toothpaste thickening systems.

[0018] (2) In terms of molecular structure, the β-(1→4)-glucan backbone of Bletilla striata polysaccharide and the helical structure of xanthan gum are cross-linked through hydrogen bonds and hydrophobic interactions to form a dynamic and reversible three-dimensional network, which gives the system excellent thixotropy (thixotropic loop area 620 Pa·s -1 About 55% higher than that of single xanthan gum), its shear thinning properties (0.1s -1 The lower viscosity is about 12,000mPa·s, 100s -1 The viscosity of the toothpaste is precisely matched to its usage scenarios – it maintains its paste shape under low shear conditions and is easy to extrude under high shear conditions. The flexible peptide chains of collagen can significantly increase the zero-shear viscosity by filling the network gaps.

[0019] (3) In terms of functional activity, the phenolic hydroxyl group of Bletilla striata polysaccharide accelerates coagulation through a charge neutralization mechanism (the coagulation time in the rat tail bleeding model can be shortened by about 30%), and its β-glucan can inhibit the formation of bacterial biofilms; the RGD sequence of collagen can significantly promote the migration of gingival fibroblasts and the repair of oral mucosa, and synergistically prolong the moistening time of the mouth with the water retention of Bletilla striata polysaccharide (>98%).

[0020] (4) In terms of process and stability, the present invention avoids collagen denaturation by premixing at low temperature (65-70°C) and controlling the pH value (6.5-7.0), allowing the system to maintain a viscosity of more than 95% after 90 days of storage at 40°C / 75% RH. In addition, the neutral pH design is effectively compatible with additives such as fluoride and potassium nitrate (viscosity fluctuation is less than 5% after adding NaF).

[0021] In summary, the present invention takes natural ingredients as the core and achieves a triple breakthrough in "performance-efficacy-environmental protection" through global optimization of structure-function-process, providing a new technical path for functional toothpaste. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] The drawings described herein are used to provide a further understanding of the present invention, constitute a part of this application, and do not constitute an improper limitation of the present invention. In the drawings: Figure 1 Schematic diagram of thixotropy test results of Example 1 and Comparative Example 1 of the present invention; Figure 2 Schematic diagram of shear thinning performance test results of Example 1 and Comparative Example 1 of the present invention; Figure 3These are photos of the hemostatic effects of Example 1 and Comparative Example 1 of the present invention; Figure 4 Schematic diagram of the hemostatic performance test results of Example 1 and Comparative Example 1 of the present invention; Figure 5 The photographs are the results of the human gingival fibroblast scratch test of Example 1 and Comparative Example 1 of the present invention; Figure 6 Schematic diagram of the test results of promoting human gingival fibroblast migration performance of Example 1 and Comparative Example 1 of the present invention; Figure 7 The following are photos of the antibacterial performance test results of Example 1 and Comparative Example 1 of the present invention. DETAILED DESCRIPTION

[0023] The present invention will be described in detail below with reference to specific embodiments and the accompanying drawings. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention but are not intended to limit the present invention.

[0024] Unless otherwise specified, the raw materials used in the examples and comparative examples are conventional commercially available raw materials, and the process methods used in the examples and comparative examples are conventional methods in the art unless otherwise specified.

[0025] Some of the raw materials used in the examples and comparative examples are described as follows: Bletilla striata polysaccharide: purchased from Xi'an Daqin Herbal Industry Co., Ltd., trade name Bletilla striata extract, packaging specification 200g / bag, molecular weight 50-200kDa (GPC determination); Xanthan gum: purchased from CP Kelco, trade name KELTROL® HP; Recombinant humanized type III collagen: purchased from Peptide Source (Guangzhou) Biotechnology Co., Ltd., the trade name is Rechprotein® COL-3 (Type X), the packaging specification is 1.6 kg / bottle.

[0026] Example 1 A toothpaste thickener based on a collagen-bletilla polysaccharide-xanthan gum composite system is prepared from the following raw materials in the following mass percentages: 2% bletilla polysaccharide, 1.2% xanthan gum, 0.6% recombinant type III humanized collagen, and the remainder being deionized water. The sum of the mass percentages of the raw materials is 100%.

[0027] The preparation method of Example 1 comprises the following steps: S1. Weigh the raw materials according to mass percentage, mix Bletilla striata polysaccharide, xanthan gum and half the mass of deionized water, stir at 65 ° C and 400 rpm for 30 min, and cool to 35 ° C to obtain a mixed solution A; S2. Dissolve recombinant humanized type III collagen in the remaining deionized water at 38°C, and adjust the pH to 6.8 using a citric acid / sodium citrate buffer system composed of citric acid and sodium citrate in a mass ratio of 2:3 to obtain a collagen solution; S3. The collagen solution obtained in step S2 is added to the mixed solution A obtained in step S1 to obtain a mixed solution B. The mixed solution B is homogenized three times under a pressure of 30 MPa, and then vacuum degassed under a pressure of -0.08 MPa for 15 minutes to obtain a toothpaste thickener based on a collagen-bletilla striata polysaccharide-xanthan gum composite system. Example 2 A toothpaste thickener based on a collagen-bletilla polysaccharide-xanthan gum composite system is prepared from the following raw materials in the following mass percentages: 2% bletilla polysaccharide, 0.8% xanthan gum, 0.4% recombinant type III humanized collagen, and the remainder being deionized water. The sum of the mass percentages of the raw materials is 100%.

[0028] The preparation method of Example 2 is the same as that of Example 1.

[0029] Example 3 A toothpaste thickener based on a collagen-bletilla polysaccharide-xanthan gum composite system is prepared from the following raw materials in the following mass percentages: 2% bletilla polysaccharide, 1.5% xanthan gum, 0.9% recombinant type III humanized collagen, and the remainder being deionized water. The sum of the mass percentages of the raw materials is 100%.

[0030] The preparation method of Example 3 is the same as that of Example 1.

[0031] Example 4 A toothpaste thickener based on a collagen-bletilla polysaccharide-xanthan gum composite system is prepared from the following raw materials in the following mass percentages: 0.5% bletilla polysaccharide, 0.3% xanthan gum, 0.1% recombinant type III humanized collagen, and the balance being deionized water. The sum of the mass percentages of the raw materials is 100%.

[0032] The preparation method of Example 4 comprises the following steps: S1. Weigh the raw materials according to mass percentage, mix Bletilla striata polysaccharide, xanthan gum and half the mass of deionized water, stir at 60 ° C and 500 rpm for 20 min, and cool to 35 ° C to obtain a mixed solution A; S2. Dissolve recombinant humanized type III collagen in the remaining deionized water at 36°C, and adjust the pH to 6.5 using a citric acid / sodium citrate buffer system composed of citric acid and sodium citrate in a mass ratio of 2:3 to obtain a collagen solution; S3. The collagen solution obtained in step S2 is added to the mixed solution A obtained in step S1 to obtain a mixed solution B. The mixed solution B is homogenized three times under a pressure of 20 MPa, and then vacuum degassed under a pressure of -0.08 MPa for 10 minutes to obtain a toothpaste thickener based on a collagen-bletilla striata polysaccharide-xanthan gum composite system.

[0033] Example 5 A toothpaste thickener based on a collagen-bletilla polysaccharide-xanthan gum composite system is prepared from the following raw materials in the following mass percentages: 5% bletilla polysaccharide, 3% xanthan gum, 2% recombinant humanized type III collagen, and the balance being deionized water. The sum of the mass percentages of the raw materials is 100%.

[0034] The preparation method of Example 5 comprises the following steps: S1. Weigh the raw materials according to mass percentage, mix Bletilla striata polysaccharide, xanthan gum and half the mass of deionized water, stir at 70 ° C and 300 rpm for 40 min, and cool to 35 ° C to obtain a mixed solution A; S2. Dissolve recombinant humanized type III collagen in the remaining deionized water at 40°C, and adjust the pH to 7.0 using a citric acid / sodium citrate buffer system composed of a mixture of citric acid and sodium citrate in a mass ratio of 2:3 to obtain a collagen solution; S3. The collagen solution obtained in step S2 is added to the mixed solution A obtained in step S1 to obtain a mixed solution B. The mixed solution B is homogenized three times under a pressure of 40 MPa, and then vacuum degassed under a pressure of -0.08 MPa for 20 minutes to obtain a toothpaste thickener based on a collagen-bletilla striata polysaccharide-xanthan gum composite system.

[0035] Comparative Example 1 The difference from Example 1 is that the raw materials do not include Bletilla striata polysaccharide and recombinant humanized type III collagen.

[0036] Comparative Example 2 The difference from Example 1 is that the Bletilla striata polysaccharide in the raw material is replaced by sodium carboxymethyl cellulose.

[0037] Comparative Example 3: The difference from Example 1 is that the pH value in step S2 is 5.0.

[0038] Test Example 1: Comparison of the stability of various formulation systems During the preparation process, it was found that the recombinant type III humanized collagen in the toothpaste thickener system prepared in Comparative Example 3 was directly precipitated, and the system was visibly uneven. Therefore, compared with other formulas, this formula system is extremely unstable, indicating that the pH value control adopted in the present invention is beneficial to the stability of the formula system.

[0039] Test Example 2: Long-term stability test 2.1 High temperature and high humidity test (1) The viscosity retention rate of the sample prepared in Example 1 of the high temperature and high humidity test (40°C / 75% RH, 90 days) was tested using a Brookfield DV2T viscometer. The test parameters are shown in Table 1: Table 1

[0040] Calculation formula: Retention rate (%) = (viscosity at test point / initial viscosity) × 100% (2) Phase separation observation (visual inspection + centrifugation method) was used to conduct stability tests on the samples prepared in Example 1 in the high temperature and high humidity test (40°C / 75% RH, 90 days). The test results are shown in Table 2: Table 2

[0041] It can be seen from Table 2 that after the high temperature and high humidity test (40° C. / 75% RH, 90 days), the viscosity retention rate of Example 1 is greater than 95%, and there is no phase separation.

[0042] 2.2 Freeze-thaw cycle test The samples prepared in Example 1 were subjected to a freeze-thaw cycle test (-20~25°C, 5 times). After freeze-thaw, the samples were visually observed and then subjected to a thixotropy test (TA Instruments DHR-2 rheometer). The test results are shown in Table 3: Table 3

[0043] It can be seen from Table 3 that the paste structure of the sample in Example 1 remained intact during repeated freezing and thawing, and the thixotropy did not decrease significantly.

[0044] It can be seen from the above experiments that the toothpaste thickener prepared by the present invention has good long-term stability.

[0045] Test Example 3: Rheological properties test 3.1 Thixotropy test The rheological properties of Example 1 (Sample A) and Comparative Example 1 (Sample B) were tested using a TA Instruments DHR-2 rheometer. The shear rate was increased from 0 to 100s. -1 , then from 100S -1 Drop to 0, collect shear stress value to calculate thixotropic ring area, the test results are as follows Figure 1 shown.

[0046] Depend on Figure 1 It can be seen that the thixotropic ring area of Example 1 is 620 Pa·s -1, compared with the single xanthan gum system (the thixotropic ring area of comparative example 1 is 401 Pa·s -1 ) increased by about 55%.

[0047] 3.2 Shear thinning performance test The rheological experiment was carried out on the sample prepared in Example 1 using a TA Instruments DHR-2 rheometer. The shear rate was changed from 0.1s -1 Increase to 100s -1 The apparent viscosity of the samples was collected during the process. The relationship between shear rate and apparent viscosity is shown in Table 4 and Figure 2 shown.

[0048] Table 4

[0049] From Table 4 and Figure 2 It can be seen that Example 1 has shear thinning properties, meeting the dual requirements of toothpaste extrusion (high shear) and paste retention (low shear).

[0050] It can be seen from the above experiments that the toothpaste thickener prepared by the present invention has good thixotropy and shear thinning properties.

[0051] Test Example 4: Hemostatic Performance Test The rat tail-cut bleeding model was used to evaluate the hemostatic effect and hemostatic performance of Example 1 and Comparative Example 1.

[0052] Hemostatic effect Figure 3 As shown, Figure 3 BSP is the sample prepared in Comparative Example 1, and BSP / XG / COL is the sample prepared in Example 1. Figure 3 It can be seen that the hemostatic effect of Example 1 is better than that of Comparative Example 1.

[0053] Hemostatic properties (clotting time comparison) such as Figure 4 As shown, Figure 4 BSP is the sample prepared in Comparative Example 1, and BSP / XG / COL is the sample prepared in Example 1. Figure 4 It can be seen that the coagulation time is shortened from 180±15 s in Comparative Example 1 to 125±10 s in Example 1 (p<0.01). Therefore, compared with Comparative Example 1, Example 1 has good hemostatic performance.

[0054] It can be seen from the above experiments that the toothpaste thickener prepared by the present invention has good hemostatic performance.

[0055] Test Example 5: Oral Restoration Test Human gingival fibroblasts were used as test cells to test the effects of Example 1 and Comparative Example 1 on promoting the migration of human gingival fibroblasts.

[0056] The results of the human gingival fibroblast scratch test are as follows Figure 5 As shown, Figure 5 BSP is the sample prepared in Comparative Example 1, and BSP / XG / COL is the sample prepared in Example 1. Figure 5 It can be seen that the repair effect of Example 1 is better than that of Comparative Example 1.

[0057] The results of the human gingival fibroblast migration test were as follows Figure 6 As shown, Figure 6 BSP is the sample prepared in Comparative Example 1, and BSP / XG / COL is the sample prepared in Example 1. Figure 6 It can be seen that 24 hours after administration of Example 1, the migration rate of human gingival fibroblasts increased to 67.20±3.39% (the comparison example was 27.38±4.00%), proving that Example 1 has more significant cell migration promoting ability and oral repair performance.

[0058] It can be seen from the above experiments that the toothpaste thickener prepared by the present invention has good cell migration promoting performance and oral repair performance.

[0059] Test Example 6: Antibacterial Performance Test The inhibition zone method was used to test the antibacterial effects of Example 1 and Comparative Example 1 on Staphylococcus aureus. The test results are as follows: Figure 7 As shown, Figure 7 The inhibition zone in the upper middle section is comparative example 1, the inhibition zone in the middle section is water (negative control), and the inhibition zone in the lower section is example 1. Figure 7 It can be seen that compared with the negative control, both Comparative Example 1 and Example 1 have certain antibacterial properties, wherein the inhibition zone of Example 1 is much larger than that of Comparative Example 1, indicating that Example 1 has stronger antibacterial properties.

[0060] It can be seen from the above experiments that the toothpaste thickener prepared by the present invention has good antibacterial properties.

[0061] The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the present invention. Anyone skilled in the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by one of ordinary skill in the art without departing from the spirit and technical principles disclosed herein are intended to be covered by the claims of the present invention.

Claims

1. A toothpaste thickener based on a collagen-bletilla striata polysaccharide-xanthan gum composite system, characterized in that: The invention is prepared from the following raw materials in the following mass percentages: 0.5-5% of bletilla striata polysaccharide, 0.3-3% of xanthan gum, 0.1-2% of collagen, and the balance being deionized water, and the sum of the mass percentages of the raw materials is 100%.

2. The toothpaste thickener based on the collagen-bletilla striata polysaccharide-xanthan gum composite system according to claim 1, characterized in that: The ratio between the sum of the mass of the xanthan gum and collagen and the mass of the bletilla striata polysaccharide is (0.6-1.2):

1.

3. The toothpaste thickener based on the collagen-bletilla striata polysaccharide-xanthan gum composite system according to claim 1, characterized in that: The molecular weight of the bletilla striata polysaccharide is 50-200 kDa.

4. The toothpaste thickener based on the collagen-bletilla striata polysaccharide-xanthan gum composite system according to claim 1, characterized in that: The xanthan gum is KELTROL® HP from CP Kelco.

5. The toothpaste thickener based on the collagen-bletilla striata polysaccharide-xanthan gum composite system according to claim 1, characterized in that: The collagen is recombinant type III humanized collagen.

6. The method for preparing a toothpaste thickener based on a collagen-bletilla striata polysaccharide-xanthan gum composite system according to any one of claims 1 to 5, characterized in that: The following steps are involved: S1. Weigh the raw materials according to mass percentage, mix Bletilla striata polysaccharide, xanthan gum and half the mass of deionized water, stir at 60-70 ℃ for 20-40min, and cool to 35 ℃ to obtain a mixed solution A; S2. Dissolve the collagen in the remaining deionized water at 36-40°C and adjust the pH to 6.5-7.0 to obtain a collagen solution; S3. Add the collagen solution obtained in step S2 to the mixed solution A obtained in step S1 to obtain a mixed solution B. Homogenize the mixed solution B three times and then vacuum degas for 10-20 minutes to obtain a toothpaste thickener based on a collagen-bletilla striata polysaccharide-xanthan gum composite system.

7. The method for preparing a toothpaste thickener based on a collagen-bletilla striata polysaccharide-xanthan gum composite system according to claim 6, characterized in that: In step S1, the stirring speed is 300-500 rpm.

8. The method for preparing a toothpaste thickener based on a collagen-bletilla striata polysaccharide-xanthan gum composite system according to claim 6, characterized in that: In step S2, the pH value is adjusted using a citric acid / sodium citrate buffer system prepared by mixing citric acid and sodium citrate in a mass ratio of 2:

3.

9. The method for preparing a toothpaste thickener based on a collagen-bletilla striata polysaccharide-xanthan gum composite system according to claim 6, characterized in that: In step S3, the homogenization pressure is 20-40 MPa, and the vacuum degassing pressure is -0.08 MPa.

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