Lettuce growth-promoting microbial agent and lettuce planting method based on agriculture and light complementation
Through lettuce proliferation microbial bacteria agent and specific planting methods, the problems of weak light and low soil quality under photovoltaic panels are solved, the photosynthesis and growth and development of lettuce are improved, and yield and quality are improved.
Patent Information
- Application Number
- CN202510637613.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-05-19
- Publication Date
- 2025-08-19
AI Technical Summary
The light intensity under the photovoltaic panels is weak and the quality of cultivated land is low, which cannot meet the planting needs of lettuce.
Lettuce-promoting microbial agents, including Bacillus subtilis, Bacillus polymyxa, Pseudomonas marshes, Flexible Cyclone and Bacillus nitrosum, combined with organic fertilizer and nutrient solution for a specific planting method.
Significantly enhance the photosynthesis of lettuce, improve soil structure and nutritional status, improve lettuce production and quality, and reduce nitrate nitrogen absorption.
Smart Images
Figure CN120505231A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of vegetable planting, and in particular relates to a lettuce growth-promoting microbial agent and a lettuce planting method based on agricultural and photoelectric complementarity. Background Art
[0002] Photovoltaic power generation is one of the most important avenues for my country's green development of clean energy. Installing photovoltaic panels at appropriate heights and spacing on ordinary farmland can generate electricity, meeting the growing demand for power generation capacity driven by rapid economic and social development. Meanwhile, through appropriate crop and variety selection and planting methods, it can minimize the impact on crop production, creating a photovoltaic-agricultural coupling system similar to vertical agriculture.
[0003] The original intention of the agricultural-photovoltaic coupling model was to maximize the use of farmland, generating revenue from the panels while simultaneously growing and selling crops below them. However, this model suffers from weak light intensity beneath the panels and poor farmland quality, which severely limits crop growth.
[0004] Lettuce, also known as glass lettuce or leaf lettuce, is a variety of Lactuca sativa in the Asteraceae family. Most lettuces are annual or biennial herbs. They have a shallow, dense root system with well-developed fibrous roots and primarily grow within the first 20 centimeters of soil. Lettuce requires high environmental conditions: ample sunlight, avoidance of shade, sufficient water, and high soil fertility. A photovoltaic coupled agricultural system cannot meet the growing requirements of lettuce. Summary of the Invention
[0005] In view of this, the purpose of the present invention is to provide a lettuce growth-promoting microbial agent and a lettuce planting method based on agricultural and photoelectric complementarity, so as to solve the problems of weak light intensity under photovoltaic panels, low quality of cultivated land, and inability to meet the planting needs of lettuce.
[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:
[0007] The invention provides a lettuce growth-promoting microbial agent, which comprises Bacillus subtilis, Paenibacillus polymyxa, Rhodopseudomonas palustris, Aconitiformis tenuissima and Nitrogen-fixing Bacterium chlorosphaer.
[0008] Preferably, the mass ratio of Bacillus subtilis, Paenibacillus polymyxa, Rhodopseudomonas palustris, Asessile Aspergillus niger and Azotobacter chrysogenum is 1-3:2-4:2-4:4-6:1-3.
[0009] Preferably, the initial concentration of the Bacillus subtilis is 3 to 5×10 10 cfu / g; the initial concentration of the polymyxa is 1 to 3×10 11cfu / g; the initial concentration of Rhodopseudomonas palustris is 1 to 3×10 9 cfu / g; the initial concentration of the fine concave spores of the ... 9 cfu / g.
[0010] The present invention also provides a lettuce planting method based on agricultural and photoelectric complementarity, comprising the following steps:
[0011] 1) soaking lettuce seeds in a seed soaking agent, drying them in the air, sowing them on a seedling substrate, covering them with soil, and cultivating them to obtain seedlings;
[0012] 2) plowing the land under the photovoltaic panels and applying organic fertilizer from lettuce leaves; transplanting the seedlings when they have 4 to 6 true leaves, and applying the lettuce growth-promoting microbial agent at the same time;
[0013] 3) Spray nutrient solution 7 to 10 days and 15 to 20 days after transplanting.
[0014] Preferably, the tail vegetable organic fertilizer in step 2) comprises the following ingredients in parts by weight: 40 to 50 parts of tail vegetables, 20 to 30 parts of straw, 15 to 25 parts of livestock and poultry manure, 12 to 18 parts of humic acid, 10 to 15 parts of blast furnace slag, 10 to 15 parts of vinegar tank, 5 to 8 parts of biochar and 3 to 5 parts of microbial agent; the application amount of the tail vegetable organic fertilizer is 400 to 500 kg / mu.
[0015] Preferably, the microbial agent includes Bacillus licheniformis, Bacillus mucilaginosus, Cellulomonas flavogens, Streptomyces tenuiflavos, Trichoderma harzianum and Pseudonocardia, and the mass ratio of Bacillus licheniformis, Bacillus mucilaginosus, Cellulomonas flavogens, Streptomyces tenuiflavos, Trichoderma harzianum and Pseudonocardia is 1-3:0.5-1.5:0.5-1.5:2-4:1-3:0.2-0.4.
[0016] Preferably, the tillage depth is 40 to 50 cm, and the application amount of the lettuce growth-promoting microbial agent is 1 to 3 mL / plant / time.
[0017] Preferably, the nutrient solution in step 3) comprises the following ingredients in concentrations: 1-5 g / L seaweed extract, 0.5-2 g / L amino acids, 0.5-2 mg / L zinc sulfate, 2-3 mg / L boric acid, 0.03-0.08 mg / L brassinolide, and 0.03-0.1 g / L rhamnolipid; the spraying amount of the nutrient solution is 3-5 mL / plant / time.
[0018] Preferably, the seed soaking agent in step 1) comprises the following components at the following concentrations: 1-3 mg / L GA3 and 10-50 mg / L NAA; the soaking temperature is 24-26° C., and the soaking time is 4-6 h.
[0019] Preferably, in step 1), the seedling matrix comprises the following ingredients in parts by weight: 10 to 15 parts of peat, 5 to 10 parts of vermiculite, 4 to 7 parts of slag ash and 1 to 3 parts of organic fertilizer, the moisture content of the seedling matrix is 60 to 70%; the thickness of the covering soil is 0.3 to 0.7 cm, and the cultivation temperature is 18 to 22°C.
[0020] Compared with the prior art, the present invention has the following beneficial effects:
[0021] The present invention significantly enhances the photosynthesis of lettuce through the synergistic cooperation of various strains in the lettuce growth-promoting microbial inoculant, overcoming the problem of weak light intensity under photovoltaic panels; by applying organic fertilizer from tail vegetables, the soil structure can be improved, soil nutrients can be increased, soil microbial activity can be enhanced, water and fertilizer retention capacity can be enhanced, and soil pH can be adjusted, overcoming the problem of poor quality of cultivated land under photovoltaic panels; by applying nutrient solution instead of nitrogen fertilizer for topdressing lettuce, the absorption and accumulation of nitrate nitrogen in lettuce can be significantly reduced. The present invention's lettuce planting method based on agricultural and photovoltaic complementarity greatly improves the photosynthesis and growth of lettuce under photovoltaic panels, and increases the yield and quality of lettuce. BRIEF DESCRIPTION OF THE DRAWINGS
[0022] Figure 1 This is a general view of the Sixin Photovoltaic Power Station in Bangluo Town, Tongwei County;
[0023] Figure 2 Schematic diagram of the cultivated land beneath the panels of the Sixin Photovoltaic Power Station in Bangluo Town, Tongwei County. DETAILED DESCRIPTION
[0024] The invention provides a lettuce growth-promoting microbial agent, which comprises Bacillus subtilis, Paenibacillus polymyxa, Rhodopseudomonas palustris, Aconitiformis tenuissima and Nitrogen-fixing Bacterium chlorosphaer.
[0025] In the present invention, the mass ratio of the Bacillus subtilis, Paenibacillus polymyxa, Rhodopseudomonas palustris, Aconitiformis spp. and Azotobacter chrysogenum is preferably 1-3:2-4:2-4:4-6:1-3, and more preferably 1.5-2.5:2.5-3.5:2.5-3.5:4.5-5.5:1.5-2.5; the initial concentration of the Bacillus subtilis is preferably 3-5×10 10 cfu / g, more preferably 3.5 to 4.5×10 10 cfu / g; the initial concentration of the polymyxa is preferably 1 to 3×10 11cfu / g, more preferably 1.5 to 2.5×10 11 cfu / g; the initial concentration of Rhodopseudomonas palustris is preferably 1 to 3×10 9 cfu / g, more preferably 1.5 to 2.5×10 9 cfu / g; the initial concentration of the fine concave spores is preferably 3000 to 8000 spores / g, more preferably 4000 to 7000 spores / g; the initial concentration of the brown ball nitrogen-fixing bacteria is preferably 5 to 8×10 9 cfu / g, more preferably 6 to 7×10 9 cfu / g.
[0026] The present invention also provides a lettuce planting method based on agricultural and photoelectric complementarity, comprising the following steps:
[0027] 1) soaking lettuce seeds in a seed soaking agent, drying them in the air, sowing them on a seedling substrate, covering them with soil, and cultivating them to obtain seedlings;
[0028] 2) plowing the land under the photovoltaic panels and applying organic fertilizer from lettuce leaves; transplanting the seedlings when they have 4 to 6 true leaves, and applying the lettuce growth-promoting microbial agent at the same time;
[0029] 3) Spray nutrient solution 7 to 10 days and 15 to 20 days after transplanting.
[0030] In the present invention, lettuce seeds are soaked in a seed soaking agent, dried, sown on a seedling substrate, covered with soil, and cultivated to obtain seedlings. The seed soaking agent includes the following components in concentrations: 1-3 mg / L GA3 and 10-50 mg / L NAA; the concentration of the GA3 is preferably 1.5-2.5 mg / L; the concentration of the NAA is preferably 20-40 mg / L; the soaking temperature is preferably 24-26°C, more preferably 25°C; the soaking time is preferably 4-6 hours, more preferably 5 hours; the sowing amount of the lettuce seeds is preferably 3-5 g / m 2 , more preferably 4g / m 2 ; The seedling matrix includes the following ingredients in parts by weight: 10-15 parts of peat, 5-10 parts of vermiculite, 4-7 parts of slag ash and 1-3 parts of organic fertilizer, the amount of the peat is preferably 11-14 parts; the amount of the vermiculite is preferably 6-9 parts; the amount of the slag ash is preferably 5-6 parts; the amount of the organic fertilizer is preferably 2 parts; the moisture content of the seedling matrix is preferably 60-70%, more preferably 62-68%; the thickness of the covering soil is preferably 0.3-0.7 cm, more preferably 0.4-0.6 cm; the cultivation temperature is preferably 18-22°C, more preferably 19-21°C.
[0031] In the present invention, the land under the photovoltaic panels is plowed and organic fertilizer of tail vegetables is applied; when the seedlings grow 4 to 6 true leaves, they are transplanted, and the lettuce growth-promoting microbial agent is applied at the same time. The plowing depth is preferably 40 to 50 cm, more preferably 42 to 48 cm; the organic fertilizer of tail vegetables includes the following ingredients in parts by weight: 40 to 50 parts of tail vegetables, 20 to 30 parts of straw, 15 to 25 parts of livestock and poultry manure, 12 to 18 parts of humic acid, 10 to 15 parts of blast furnace slag, 10 to 15 parts of vinegar tank, 5 to 8 parts of biochar and 3 to 5 parts of microbial agent; the amount of tail vegetables is preferably 42 to 48 parts; the amount of straw is preferably 22 to 2 8 parts; the amount of livestock and poultry manure is preferably 18-22 parts; the amount of humic acid is preferably 14-16 parts; the amount of blast furnace slag is preferably 11-14 parts; the amount of vinegar tank is preferably 11-14 parts; the amount of biochar is preferably 6-7 parts; the amount of microbial agent is preferably 4 parts; the microbial agent includes Bacillus licheniformis, Bacillus mucilaginosus, Cellulomonas flavogenes, Streptomyces tenuifolius, Trichoderma harzianum and Pseudonocardia, the lichen The mass ratio of Bacillus, Bacillus mucilaginosus, Xanthocellomonas, Streptomyces tenuifolius, Trichoderma harzianum and Pseudonocardia is preferably 1-3: 0.5-1.5: 0.5-1.5: 2-4: 1-3: 0.2-0.4, and more preferably 1.5-2.5: 0.8-1.2: 0.8-1.2: 2.5-3.5: 1.5-2.5: 0.25-0.35; the preparation method of the tail vegetable organic fertilizer comprises the following steps: 1) 1) Dehydrate the vegetables to a water content of 55-60%, and collect the dehydrated vegetables and residual liquid; 2) Mix straw, livestock and poultry manure, humic acid and vinegar tank to obtain a mixture, and use the residual liquid of the vegetables to adjust the water content of the mixture to 50-55% to obtain a treated mixture; mix the treated mixture, blast furnace slag and biochar to obtain a fermented material; 3) Mix the fermented material and microbial agent to obtain a fertilizer pile, ferment for 20-30 days, and obtain organic fertilizer for vegetables. The application rate of the organic fertilizer for vegetables is preferably 400-500 kg / mu, and more preferably 420-480 kg / mu. The row spacing for transplanting is preferably 20-30 cm × 20-30 cm, and more preferably 22-28 cm × 22-28 cm; the application rate of the lettuce growth-promoting microbial agent is preferably 1-3 mL / plant / time, and more preferably 1.5-2.5 mL / plant / time.
[0032] In the present invention, a nutrient solution is sprayed 7 to 10 days and 15 to 20 days after transplanting, respectively. The nutrient solution comprises the following components at the following concentrations: 1 to 5 g / L seaweed extract, 0.5 to 2 g / L amino acids, 0.5 to 2 mg / L zinc sulfate, 2 to 3 mg / L boric acid, 0.03 to 0.08 mg / L brassinolide, and 0.03 to 0.1 g / L rhamnolipid; the concentration of the seaweed extract is preferably 2 to 4 g / L; the concentration of the amino acids is preferably 0.8 to 1.5 g / L; the concentration of the zinc sulfate is preferably 0.8 to 1.5 mg / L; the concentration of the brassinolide is preferably 0.04 to 0.07 mg / L; the concentration of the rhamnolipid is preferably 0.04 to 0.08 g / L; and the spraying amount of the nutrient solution is preferably 3 to 5 mL / plant / time, more preferably 3.5 to 4.5 mL / plant / time.
[0033] The technical solutions provided by the present invention are described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.
[0034] Bacillus subtilis was purchased from Weifang Yihao Biotechnology Co., Ltd.; Bacillus polymyxa, Rhodopseudomonas palustris, Streptomyces tenuifolius, and Trichoderma harzianum were purchased from Guangzhou Zhenwei Microbiology Technology Co., Ltd.; Aconitiformis tenuifolia was purchased from the Institute of Root Biology of Yangtze University; Bacillus licheniformis, Azotobacter chrysogenum, Cellulomonas flavigens, and Pseudonocardia were purchased from Wuhan Huizao Biotechnology Co., Ltd.; Bacillus mucilaginosus was purchased from Shanghai Baocang Microbiology Co., Ltd.
[0035] Example 1
[0036] The invention discloses a tail vegetable organic fertilizer comprising the following components in parts by weight: 45 parts of tail vegetables, 25 parts of straw, 20 parts of livestock and poultry manure, 14 parts of humic acid, 12 parts of blast furnace slag, 12 parts of vinegar trough, 6 parts of biochar and 4 parts of microbial agent (the mass ratio of Bacillus licheniformis, Bacillus mucilaginosus, Cellulomonas flavogenes, Streptomyces tenuiflavos, Trichoderma harzianum and Pseudonocardia is 2:1:1:3:2:0.3). The preparation method of the tail vegetable organic fertilizer is as follows: dehydrating the tail vegetables to a water content of 58%, collecting the dehydrated tail vegetables and tail vegetable residual liquid; mixing the straw, livestock and poultry manure, humic acid and vinegar trough to obtain a mixture, adjusting the water content of the mixture to 52% using the tail vegetable residual liquid to obtain a treated mixture; mixing the treated mixture, blast furnace slag and biochar to obtain a fermentation product; mixing the fermentation product and the microbial agent to obtain a compost pile, and fermenting for 25 days to obtain the tail vegetable organic fertilizer.
[0037] The lettuce planting method based on agricultural and light complementation is as follows: lettuce seeds are soaked in a seed soaking agent (2 mg / L GA3 and 30 mg / L NAA) at 25 ° C for 5 hours and then dried. 2The seeds were sown on a seedling medium (13 parts peat, 8 parts vermiculite, 5 parts slag ash and 2 parts organic fertilizer, with a water content of 65%), covered with 0.5 cm of soil, and cultivated at a temperature of 20°C to obtain seedlings; the land under the photovoltaic panel was plowed 45 cm, and 450 kg / mu of tail vegetable organic fertilizer was applied and mixed evenly with the soil; when the seedlings grew 5 true leaves, they were transplanted, and the transplanting row spacing was 25 cm × 25 cm. At the same time, a lettuce growth-promoting microbial agent (4 × 10 10 cfu / g Bacillus subtilis, 2×10 11 cfu / g Paenibacillus polymyxa, 2×10 9 cfu / g Rhodopseudomonas palustris, 5000 spores / g Aspergillus niger and 6×10 9 cfu / g brown ball nitrogen-fixing bacteria were mixed in the mass ratio of 2:3:3:5:2); then, nutrient solution (3g / L seaweed extract, 1g / L amino acid, 1mg / L zinc sulfate, 2.5mg / L boric acid, 0.05mg / L brassinolide and 0.05g / L rhamnolipid) was sprayed at a rate of 4mL / plant / time on days 8 and 17 after transplanting.
[0038] Example 2
[0039] The invention discloses a tail vegetable organic fertilizer comprising the following components in parts by weight: 40 parts of tail vegetables, 20 parts of straw, 15 parts of livestock and poultry manure, 12 parts of humic acid, 10 parts of blast furnace slag, 10 parts of vinegar trough, 5 parts of biochar and 3 parts of microbial agent (the mass ratio of Bacillus licheniformis, Bacillus mucilaginosus, Cellulomonas flavogenes, Streptomyces tenuiflavos, Trichoderma harzianum and Pseudonocardia is 1:0.5:0.5:2:1:0.2). The preparation method of the tail vegetable organic fertilizer is as follows: dehydrating the tail vegetables to a water content of 55%, collecting the dehydrated tail vegetables and tail vegetable residual liquid; mixing the straw, livestock and poultry manure, humic acid and vinegar trough to obtain a mixture, and using the tail vegetable residual liquid to adjust the water content of the mixture to 50% to obtain a treated mixture; mixing the treated mixture, blast furnace slag and biochar to obtain a fermentation product; mixing the fermentation product and the microbial agent to obtain a compost pile, and fermenting for 20 days to obtain the tail vegetable organic fertilizer.
[0040] The lettuce planting method based on agricultural and light complementation is as follows: lettuce seeds are soaked in a seed soaking agent (1 mg / L GA3 and 10 mg / L NAA) at 24 ° C for 4 hours and then dried. 2The sowing amount was sown on the seedling medium (10 parts of peat, 5 parts of vermiculite, 4 parts of slag ash and 1 part of organic fertilizer, with a water content of 60%), covered with 0.3 cm of soil, and cultivated at a temperature of 18°C to obtain seedlings; the land under the photovoltaic panel was plowed 40 cm, and 400 kg / mu of tail vegetable organic fertilizer was applied and mixed evenly with the soil; when the seedlings grew 4 true leaves, they were transplanted, and the transplanting row spacing was 20 cm × 20 cm. At the same time, the lettuce growth-promoting microbial agent (3 × 10 10 cfu / g Bacillus subtilis, 1×10 11 cfu / g Paenibacillus polymyxa, 1×10 9 cfu / g Rhodopseudomonas palustris, 3000 spores / g Aspergillus niger and 5×10 9 cfu / g brown ball nitrogen-fixing bacteria were mixed in a mass ratio of 1:2:2:4:1); then, nutrient solution (1g / L seaweed extract, 0.5g / L amino acid, 0.5mg / L zinc sulfate, 2mg / L boric acid, 0.03mg / L brassinolide and 0.03g / L rhamnolipid) was sprayed at a rate of 3mL / plant / time on days 7 and 15 after transplanting.
[0041] Example 3
[0042] The invention discloses a tail vegetable organic fertilizer comprising the following components by weight: 50 parts of tail vegetables, 30 parts of straw, 25 parts of livestock and poultry manure, 18 parts of humic acid, 15 parts of blast furnace slag, 15 parts of vinegar trough, 8 parts of biochar and 5 parts of microbial agent (the mass ratio of Bacillus licheniformis, Bacillus mucilaginosus, Cellulomonas flavogenes, Streptomyces tenuiflavos, Trichoderma harzianum and Pseudonocardia is 3:1.5:1.5:4:3:0.4). The preparation method of the tail vegetable organic fertilizer is as follows: dehydrating the tail vegetables to a water content of 60%, collecting the dehydrated tail vegetables and tail vegetable residual liquid; mixing the straw, livestock and poultry manure, humic acid and vinegar trough to obtain a mixture, adjusting the water content of the mixture to 55% using the tail vegetable residual liquid to obtain a treated mixture; mixing the treated mixture, blast furnace slag and biochar to obtain a fermentation product; mixing the fermentation product and the microbial agent to obtain a compost pile, and fermenting for 30 days to obtain the tail vegetable organic fertilizer.
[0043] The lettuce planting method based on agricultural and light complementation is as follows: lettuce seeds are soaked in a seed soaking agent (3 mg / L GA3 and 50 mg / L NAA) at 26 ° C for 6 hours and then dried. 2The sowing amount was sown on the seedling medium (15 parts of peat, 10 parts of vermiculite, 7 parts of slag ash and 3 parts of organic fertilizer, with a water content of 70%), covered with 0.7 cm of soil, and cultivated at a temperature of 22°C to obtain seedlings; the land under the photovoltaic panel was plowed 50 cm, and 500 kg / mu of tail vegetable organic fertilizer was applied and mixed evenly with the soil; when the seedlings grew 6 true leaves, they were transplanted, and the transplanting row spacing was 30 cm × 30 cm. At the same time, 2.5 mL / plant / time of lettuce growth-promoting microbial agent (5 × 10 10 cfu / g Bacillus subtilis, 3×10 11 cfu / g Paenibacillus polymyxa, 3×10 9 cfu / g Rhodopseudomonas palustris, 8000 spores / g Aspergillus niger and 8×10 9 cfu / g brown ball nitrogen-fixing bacteria were mixed in the mass ratio of 3:4:4:6:3); then, nutrient solution (5g / L seaweed extract, 2g / L amino acid, 2mg / L zinc sulfate, 3mg / L boric acid, 0.08mg / L brassinolide and 0.1g / L rhamnolipid) was sprayed at a rate of 5mL / plant / time on the 10th and 20th days after transplanting.
[0044] Comparative Example 1
[0045] The difference from Example 1 is that no lettuce growth-promoting microbial agent was applied.
[0046] Comparative Example 2
[0047] The difference from Example 1 is that the tail vegetable organic fertilizer is replaced by commercially available organic fertilizer (purchased from Fadada Bio-fermentation Fertilizer Co., Ltd., Gaocheng District, Shijiazhuang City).
[0048] Comparative Example 3
[0049] The difference from Example 1 is that the nutrient solution was replaced with commercially available NutriGrow high-nitrogen liquid fertilizer (purchased from Greenmei (Suzhou) Agricultural Technology Co., Ltd.).
[0050] Comparative Example 4
[0051] The difference from Example 1 is that the lettuce growth-promoting microbial agent is not added with Aspergillus niger.
[0052] Comparative Example 5
[0053] The difference from Example 1 is that brown-spherical nitrogen-fixing bacteria are not added to the lettuce growth-promoting microbial agent.
[0054] Comparative Example 6
[0055] The difference from Example 1 is that Rhodopseudomonas palustris is not added to the lettuce growth-promoting microbial agent.
[0056] Comparative Example 7
[0057] The difference from Example 1 is that Paenibacillus polymyxa is not added to the lettuce growth-promoting microbial agent.
[0058] Comparative Example 8
[0059] The difference from Example 1 is that Bacillus subtilis is not added to the lettuce growth-promoting microbial agent.
[0060] Comparative Example 9
[0061] The difference from Example 1 is that the lettuce growth-promoting microbial agent is not added with A. tenuissima and A. chlorosphaera.
[0062] Comparative Example 10
[0063] The difference from Example 1 is that the mass ratio of Bacillus subtilis, Paenibacillus polymyxa, Rhodopseudomonas palustris, Aspergillus niger and Azotobacter chrysogenum in the lettuce growth-promoting microbial agent is 1:1:1:3:1.
[0064] Comparative Example 11
[0065] The difference from Example 1 is that the mass ratio of Bacillus subtilis, Paenibacillus polymyxa, Rhodopseudomonas palustris, Aspergillus niger and Azotobacter chrysogenum in the lettuce growth-promoting microbial agent is 4:5:5:7:4.
[0066] Experimental Example 1
[0067] Taking the Italian year-round bolting-resistant lettuce as an example, the Sixin Photovoltaic Power Station in Bangluo Town, Tongwei County was chosen. The power station has an installed capacity of 47.2 MW and covers a total area of 1,511 acres. It was completed and connected to the grid for power generation in January 2019. The land attribute is general arable land, and a photovoltaic module array is erected on it. The photovoltaic module size is 1960mm×992mm, the single weight is 25kg, the peak power is 320Wp, and the installation angle is 26°. A single row of photovoltaic modules is arranged in the south and north to form a photovoltaic array. The array is spaced 1.75 meters apart in the north-south direction. The array adopts a steel truss support structure with a truss height of 3.95 meters. The truss supports are spaced 10 meters north-south and 4 meters east-west. The cable is buried at a depth of 0.8-1 meter. The cultivated land under the photovoltaic power station panels is the responsibility of the Gansu Energy Aid Xinglong Photovoltaic Agriculture Farmers Professional Cooperative. Figure 1 and Figure 2 shown.
[0068] Lettuce was cultivated according to the methods of Examples 1-3 and Comparative Examples 1-11. All treatments were cultured until harvest, and samples were taken to measure individual plant yield (fresh weight), soluble sugar, soluble protein, vitamin C, nitrate, chlorophyll, and net photosynthetic rate. Fresh weight of the leaves was removed by absorbing residual moisture with absorbent paper and then weighed using a balance with an accuracy of 0.01 g. Soluble sugar was determined using anthrone colorimetry, soluble protein using Coomassie Brilliant Blue-G250 staining, vitamin C using 2,6-dichlorophenol indophenol titration, and nitrate nitrogen content using the salicylic acid method. Chlorophyll (chlorophyll a and chlorophyll b) was extracted with acetone, and net photosynthetic rate (Pn) was determined spectrophotometrically using an LI-6400 portable photosynthetic analyzer.
[0069] Experimental results: as shown in Table 1.
[0070] Table 1 Effects of different treatments on photosynthesis and growth of lettuce
[0071]
[0072]
[0073] As can be seen from Table 1, the yield per plant (fresh weight), soluble sugar, soluble protein, VC, chlorophyll and net photosynthetic rate of lettuce in Examples 1 to 3 are much higher than those in Comparative Examples 1 to 11, and the nitrate content is much lower than that in Comparative Examples 1 to 11. The synergistic combination of lettuce growth-promoting microbial agents, tail vegetable organic fertilizer and nutrient solution can significantly enhance the photosynthesis of lettuce, provide sufficient nutrients for the growth of lettuce, and improve the yield and quality of lettuce. The lack of any one or more strains in the lettuce growth-promoting microbial agents and the change in their dosage ratio will greatly reduce the chlorophyll content and net photosynthetic rate of lettuce, which is not conducive to photosynthesis and the synthesis of nutrients in lettuce.
[0074] From the above embodiments, comparative examples and experimental examples, it can be seen that the present invention can significantly enhance the photosynthesis of lettuce by synergizing the various strains in the lettuce growth-promoting microbial agent, thereby overcoming the problem of weak light intensity under the photovoltaic panel; by applying organic fertilizer from tail vegetables, it can improve soil structure, increase soil nutrients, increase soil microbial activity, enhance water and fertilizer retention capacity, and regulate soil pH, thereby overcoming the problem of poor quality of cultivated land under the photovoltaic panel; by applying nutrient solution instead of nitrogen fertilizer to topdress lettuce, it can significantly reduce the absorption and accumulation of nitrate nitrogen in lettuce. The lettuce planting method based on agricultural and light complementarity of the present invention greatly improves the photosynthesis and growth and development of lettuce under the photovoltaic panel, and improves the yield and quality of lettuce.
[0075] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.
Claims
1. A lettuce growth-promoting microbial agent, characterized in that: Including Bacillus subtilis, Bacillus polymyxa, Rhodopseudomonas palustris, Aspergillus niger and Azotobacter chrysogenum.
2. The lettuce growth-promoting microbial agent according to claim 1, characterized in that The mass ratio of the Bacillus subtilis, Paenibacillus polymyxa, Rhodopseudomonas palustris, Aeschylus septorius and Azotobacter chrysogenum is 1-3:2-4:2-4:4-6:1-3.
3. The lettuce growth-promoting microbial agent according to claim 1 or 2, characterized in that: The initial concentration of the Bacillus subtilis is 3 to 5×10 10 cfu / g; the initial concentration of the polymyxa is 1 to 3×10 11 cfu / g; the initial concentration of Rhodopseudomonas palustris is 1 to 3×10 9 cfu / g; the initial concentration of the fine concave spores of the ... 9 cfu / g.
4. A lettuce planting method based on agricultural and light complementarity, characterized in that: The following steps are involved: 1) soaking lettuce seeds in a seed soaking agent, drying them in the air, sowing them on a seedling substrate, covering them with soil, and cultivating them to obtain seedlings; 2) plowing the land under the photovoltaic panels and applying organic fertilizer from the tail vegetables; transplanting the seedlings when they have 4 to 6 true leaves, and applying the lettuce growth-promoting microbial agent according to any one of claims 1 to 3 at the same time; 3) Spray nutrient solution 7 to 10 days and 15 to 20 days after transplanting.
5. The lettuce planting method according to claim 4, characterized in that: Step 2) The tail vegetable organic fertilizer comprises the following ingredients in parts by weight: 40 to 50 parts of tail vegetables, 20 to 30 parts of straw, 15 to 25 parts of livestock and poultry manure, 12 to 18 parts of humic acid, 10 to 15 parts of blast furnace slag, 10 to 15 parts of vinegar tank, 5 to 8 parts of biochar and 3 to 5 parts of microbial agent; the application amount of the tail vegetable organic fertilizer is 400 to 500 kg / mu.
6. The lettuce planting method according to claim 5, characterized in that: The microbial agent includes Bacillus licheniformis, Bacillus mucilaginosus, Cellulomonas flavogens, Streptomyces tenuiflavos, Trichoderma harzianum and Pseudonocardia, and the mass ratio of Bacillus licheniformis, Bacillus mucilaginosus, Cellulomonas flavogens, Streptomyces tenuiflavos, Trichoderma harzianum and Pseudonocardia is 1-3:0.5-1.5:0.5-1.5:2-4:1-3:0.2-0.
4.
7. The lettuce planting method according to claim 4, characterized in that: Step 2) The tillage depth is 40 to 50 cm, and the application rate of the lettuce growth-promoting microbial agent is 1 to 3 mL per plant per time.
8. The lettuce planting method according to claim 4, characterized in that: Step 3) The nutrient solution comprises the following ingredients at the following concentrations: 1-5 g / L seaweed extract, 0.5-2 g / L amino acids, 0.5-2 mg / L zinc sulfate, 2-3 mg / L boric acid, 0.03-0.08 mg / L brassinolide, and 0.03-0.1 g / L rhamnolipid; the spraying amount of the nutrient solution is 3-5 mL / plant / time.
9. The lettuce planting method according to claim 4, characterized in that: Step 1) The seed soaking agent comprises the following components at the following concentrations: 1-3 mg / L GA3 and 10-50 mg / L NAA; the soaking temperature is 24-26° C., and the soaking time is 4-6 hours.
10. The lettuce planting method according to claim 4, characterized in that: Step 1) The seedling culture medium comprises the following components in parts by weight: 10 to 15 parts of peat, 5 to 10 parts of vermiculite, 4 to 7 parts of slag ash and 1 to 3 parts of organic fertilizer, the moisture content of the seedling culture medium is 60 to 70%; the thickness of the covering soil is 0.3 to 0.7 cm; and the cultivation temperature is 18 to 22°C.