Equipment and method for removing and extracting histamine of gentamicin sulfate

Abstract

The invention relates to the technical field of histamine extraction, in particular to gentamicin sulfate histamine removal and extraction equipment and method.The gentamicin sulfate histamine removal and extraction equipment comprises a drying barrel with a feeding port and a discharging port, the drying barrel is vertically arranged, a material guiding barrel is arranged in the drying barrel, and the material guiding barrel and the drying barrel are coaxially arranged; electric heating wires are arranged between the inner wall and the outer wall of the drying barrel and between the inner wall and the outer wall of the guide barrel; an outer drying cavity is formed between the outer wall of the material guiding barrel and the inner wall of the drying barrel, an inner drying cavity is formed in the material guiding barrel, the bottom of the outer drying cavity communicates with the bottom of the inner drying cavity, and the top of the outer drying cavity communicates with the top of the inner drying cavity; a spiral lifting unit is arranged in the inner drying cavity, and a rotary stirring unit is arranged in the outer drying cavity. The drying efficiency of the crystals can be improved.

Description

Technical Field

[0001] The present application relates to the technical field of histamine extraction, and in particular to a histamine removal and extraction device and method for gentamicin sulfate. Background Art

[0002] Gentamicin sulfate is clinically used to treat sepsis, respiratory tract infections, biliary tract infections, purulent peritonitis, intracranial infections, urinary tract infections, and bacillary dysentery caused by Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Shigella dysenteriae, Klebsiella pneumoniae, Proteus, and other sensitive bacteria. Gentamicin sulfate is produced through microbial fermentation. The post-fermentation extraction and purification process may produce byproducts or impurities, such as histamine. The presence of histamine can affect the purity of gentamicin sulfate, so histamine removal and extraction are necessary.

[0003] During the histamine extraction process, the gentamicin sulfate fermentation broth needs to be centrifuged to remove bacterial residues and insoluble impurities, then histamine is eluted using an ion exchange resin method, and then concentrated to obtain crude histamine. Finally, the crude histamine is crystallized and dried to obtain the finished histamine product.

[0004] Through searching, Chinese patent announcement number CN220931578U discloses a vacuum drying device for preparing acid salt, comprising a drying tower body, wherein the inner walls around the drying tower body are fixed with a placement plate by bolts, the upper surface of the placement plate is rotatably connected to a connecting shaft, and a plurality of stirring plates are welded to the circumferential outer wall of the connecting shaft, and a plurality of heating wires are provided between adjacent stirring plates, and one side of the plurality of stirring plates is rotatably connected to two spiral racks, and the thread directions of the two spiral racks are opposite. The utility model can not only change the fixed state of the crystals during drying by setting the stirring plates, so that the crystals are heated evenly, but also circulate the crystals by setting the stirring racks, thereby improving the fluidity of the crystals and making the crystals heated more evenly, and can also discharge the crystals from the drying chamber by setting the discharging mechanism, making the discharging more convenient and improving the convenience of the device.

[0005] The above-mentioned related technologies have the following defects: although the spiral rack can transport and stir the crystals, since the heating wire is arranged at the stirring plate, the crystals located between the upper and lower spiral racks and the crystals located on the inner wall of the drying tower body are not easily transported to the heating wire for drying, resulting in low drying efficiency of the crystals; in addition, during the unloading process of the crystals, the spiral rack cannot clean the crystals located between the two spiral racks and attached to the inner wall of the drying tower body, resulting in incomplete discharge of the crystals. At this time, workers need to clean them manually, which further affects the drying efficiency of the crystals, and therefore needs to be improved. Summary of the Invention

[0006] In order to improve the drying efficiency of crystals, the present application provides a histamine removal and extraction device and method for gentamicin sulfate.

[0007] In a first aspect, the present application provides a histamine removal and extraction device for gentamicin sulfate, which adopts the following technical solution: a histamine removal and extraction device for gentamicin sulfate, comprising a drying barrel having a feed inlet and a discharge outlet, the drying barrel being vertically arranged, a material guide barrel being provided within the drying barrel, the material guide barrel and the drying barrel being coaxially arranged, and electric heating wires being provided between the inner and outer walls of the drying barrel and between the inner and outer walls of the material guide barrel;

[0008] An outer drying chamber is provided between the outer wall of the material guide barrel and the inner wall of the drying barrel, and an inner drying chamber is provided in the material guide barrel. The bottom of the outer drying chamber is connected to the bottom of the inner drying chamber, and the top of the outer drying chamber is connected to the top of the inner drying chamber.

[0009] A spiral lifting unit is provided in the inner drying chamber, and a rotary stirring unit is provided in the outer drying chamber.

[0010] Optionally, the spiral lifting unit includes a driving motor provided on the drying barrel, and a spiral blade is coaxially connected to the output shaft of the driving motor, and the spiral blade is rotatably embedded in the inner drying chamber.

[0011] Optionally, the rotary stirring unit includes a stirring blade connected to the output shaft of the driving motor through a connecting plate, and the stirring blade abuts against the inner wall of the drying barrel and the outer wall of the material guiding barrel.

[0012] Optionally, it also includes an injection unit, which includes a high-pressure pump. The high-pressure pump is connected to a high-pressure nozzle through a connecting pipe. The high-pressure nozzle is provided with a plurality of high-pressure nozzles facing the axis of the drying barrel. The inner wall of the drying barrel is provided with a groove for embedding the high-pressure nozzle. The stirring blade will pass through the high-pressure nozzle during rotation.

[0013] Optionally, the end of the stirring blade that is used to abut against the inner wall of the drying barrel and the end of the stirring blade that is used to abut against the outer wall of the material guiding barrel will pass through the high-pressure nozzle successively.

[0014] Optionally, the material guide barrel is provided with an air groove, which runs through the inner and outer walls of the material guide barrel and is directly opposite to the high-pressure nozzle.

[0015] Optionally, the inner diameter of the lower portion of the drying barrel gradually decreases from top to bottom.

[0016] Optionally, a material distribution barrel is further included, wherein a feed hopper is provided on the material distribution barrel, and a material distribution plate is provided in the material distribution barrel below the feed hopper, wherein the height of the upper surface of the material distribution plate gradually decreases from the center thereof to the edge thereof;

[0017] There are multiple drying barrels and they are connected to the inside of the distribution barrel through a material guide channel. One end of the material guide channel is connected to the feed port, and the other end of the material guide channel is located at the lowest point on the upper surface of the distribution plate.

[0018] Optionally, an opening and closing unit is also included, which includes a reduction motor. The output shaft of the reduction motor is provided with a plurality of opening and closing doors for controlling the opening and closing of the discharge port, and the opening and closing doors correspond to the discharge port one by one.

[0019] In a second aspect, the present application provides a method for removing histamine from gentamicin sulfate, which adopts the following technical solution: A method for removing histamine from gentamicin sulfate comprises the following steps:

[0020] S1. Selecting a gentamicin sulfate fermentation broth with a high histamine content, removing bacterial residues and insoluble impurities by centrifugation to obtain a centrifuge;

[0021] S2, using a nanofiltration membrane to concentrate the centrifuge liquid, gentamicin sulfate is retained, and histamine passes through the nanofiltration membrane along with the centrifuge liquid to obtain a separated liquid;

[0022] S3, using a weakly acidic cation exchange resin at pH 6.0-6.5 to adsorb gentamicin sulfate in the separation solution, and histamine passes through the resin to obtain a flowthrough;

[0023] S4. Adjust the pH of the permeate to 10-11, adsorb histamine with a macroporous adsorption resin, and elute with ethanol-water (1:1). After concentration, obtain crude histamine;

[0024] S5. Concentrate the crude histamine, adjust the pH to 3.0-3.5, and allow to stand at 4°C for crystallization;

[0025] S6. Drying the crystals through a histamine removal extraction device for gentamicin sulfate.

[0026] In summary, this application has the following beneficial technical effects:

[0027] 1. When the crystals enter the outer drying chamber through the feed port, the rotary stirring unit will rotate and stir the crystals in the outer drying chamber, causing the crystals to be constantly turned over. The spiral lifting unit will spirally lift the crystals in the inner drying chamber and transport the crystals to the outer drying chamber, so that the crystals circulate in the outer and inner drying chambers. The inner wall of the drying barrel and the inner and outer walls of the material guide barrel can heat and dry the crystals, thereby improving the drying efficiency of the crystals.

[0028] 2. During the crystal discharging process, the high-pressure pump can ventilate the high-pressure nozzle through the connecting pipe. The high-pressure nozzle will spray high-pressure gas through the high-pressure nozzle, so that the crystals attached to the stirring blades and spiral blades are blown away. The scraped and blown crystals will be discharged through the discharge port, eliminating the need for workers to manually clean the residual crystals in the drying barrel, thereby improving the drying efficiency of the crystals;

[0029] 3. When the crystals enter the distribution barrel through the feed hopper, the crystals will fall to the upper surface of the distribution plate and slide down along the upper surface of the distribution plate. The crystals will fall into each drying barrel through the material guide channel, realizing automatic distribution of the crystals. Multiple drying barrels can dry the crystals together, improving the drying efficiency of the crystals. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 This is a schematic structural diagram of the drying barrel in Example 1 of the present application;

[0031] Figure 2 This is a schematic cross-sectional view of the drying barrel in Example 1 of the present application;

[0032] Figure 3 This is a schematic cross-sectional view of the internal structure of the drying barrel in Example 1 of the present application;

[0033] Figure 4 This is a schematic cross-sectional view of the drying barrel and the material guide barrel in Example 1 of the present application;

[0034] Figure 5 This is a schematic structural diagram of the material distribution barrel and the drying barrel in Example 2 of the present application;

[0035] Figure 6 This is a top view of the material distribution barrel and the drying barrel in Example 2 of the present application;

[0036] Figure 7 This is a schematic cross-sectional view of the material distribution barrel and the drying barrel in Example 2 of the present application;

[0037] Figure 8 It is a structural diagram of the opening and closing unit in Example 2 of the present application.

[0038] Figure numerals: 1. Drying barrel; 11. Feed port; 12. Discharge port; 13. External drying chamber; 14. Groove; 2. Material guide barrel; 21. Internal drying chamber; 22. Cavity; 23. Heating wire; 24. Air trough; 3. Spiral lifting unit; 31. Drive motor; 32. Spiral blade; 4. Rotary stirring unit; 41. Connecting plate; 42. Stirring blade; 421. External scraper; 422. Internal scraper; 423. Reinforcement plate; 5. Jet unit; 51. High-pressure pump; 52. Connecting pipe; 53. High-pressure nozzle; 54. High-pressure nozzle; 6. Material distribution barrel; 61. Feed hopper; 62. Material distribution plate; 63. Bracket; 64. Material guide channel; 7. Opening and closing unit; 71. Reducer motor; 72. Connecting rod; 73. Opening and closing door; 74. Guide rail; 8. PLC controller. DETAILED DESCRIPTION

[0039] The following is combined with Figure 1-8 This application is described in further detail.

[0040] Example 1

[0041] The present application discloses a histamine removal and extraction device for gentamicin sulfate. Figure 1 and Figure 2 As shown, a histamine removal and extraction device for gentamicin sulfate includes a vertically arranged drying barrel 1, a feed port 11 is provided at the top of the drying barrel 1, and a discharge port 12 is provided at the bottom of the drying barrel 1; a material guide barrel 2 is provided in the drying barrel 1, and the material guide barrel 2 is coaxially arranged with the drying barrel 1.

[0042] An annular outer drying chamber 13 is provided between the outer wall of the material guide barrel 2 and the inner wall of the drying barrel 1, and the feed port 11 is connected to the outer drying chamber 13. An inner drying chamber 21 is provided in the material guide barrel 2, and the bottom of the outer drying chamber 13 is connected to the bottom of the inner drying chamber 21, and the top of the outer drying chamber 13 is connected to the top of the inner drying chamber 21.

[0043] A cavity 22 is provided between the inner wall and the outer wall of the drying barrel 1 and between the inner wall and the outer wall of the material guiding barrel 2. A heating wire 23 is welded in the cavity 22. The heating wire 23 can heat the drying barrel 1 and the material guiding barrel 2, so that the crystals in the inner drying chamber 21 and the outer drying chamber 13 can be heated and dried.

[0044] A spiral lifting unit 3 is provided in the inner drying chamber 21 . The spiral lifting unit 3 includes a driving motor 31 mounted on the top outer wall of the drying barrel 1 . A spiral blade 32 is coaxially connected to the output shaft of the driving motor 31 . The spiral blade 32 is rotatably embedded in the inner drying chamber 21 .

[0045] When the crystals enter the outer drying chamber 13 through the feed port 11, the worker will close the discharge port 12 through the outer door panel, and the crystals will fall to the connecting point between the bottom of the outer drying chamber 13 and the bottom of the inner drying chamber 21. At this time, the driving motor 31 will drive the spiral blade 32 to rotate, and the spiral blade 32 will drive the crystals at the bottom of the inner drying chamber 21 to spirally rise to the top of the inner drying chamber 21. Then the crystals will fall into the outer drying chamber 13 and continue to fall to the connecting point between the bottom of the outer drying chamber 13 and the bottom of the inner drying chamber 21, so that the crystals are always circulating in the outer drying chamber 13 and the inner drying chamber 21, and the inner wall of the drying barrel 1 and the inner and outer walls of the material guide barrel 2 can heat and dry the crystals, thereby improving the drying efficiency of the crystals.

[0046] It is worth noting that the spiral blades 32 will come into contact with the inner wall of the material guiding barrel 2 during the rotation process, so that the crystals attached to the inner wall of the material guiding barrel 2 are scraped off.

[0047] The inner diameter of the lower part of the drying barrel 1 gradually decreases from top to bottom, so that the crystals at the bottom of the outer drying chamber 13 will gather toward the bottom of the inner drying chamber 21, so that the crystals circulate in the outer drying chamber 13 and the inner drying chamber 21.

[0048] like Figures 2 to 4 As shown, a rotary stirring unit 4 is provided within the outer drying chamber 13. The rotary stirring unit 4 includes a stirring blade 42 connected to the output shaft of the drive motor 31 via a connecting plate 41. When the drive motor 31 rotates the spiral blade 32, the drive motor 31 also rotates the stirring blade 42 via the connecting plate 41. The stirring blade 42 rotates against the inner wall of the drying barrel 1 and the outer wall of the material guide barrel 2, thereby scraping off crystals adhering to the inner wall of the drying barrel 1 and the outer wall of the material guide barrel 2. In addition, the stirring blade 42 rotates and stirs the crystals within the outer drying chamber 13, causing the crystals to be continuously turned over, thereby improving the drying efficiency of the crystals.

[0049] The stirring blade 42 includes an outer scraper 421 and an inner scraper 422. The outer scraper 421 contacts the inner wall of the drying barrel 1, and the inner scraper 422 contacts the outer wall of the material guide barrel 2. The outer scraper 421 and the inner scraper 422 are connected by several horizontally arranged reinforcement plates 423. The arrangement of the reinforcement plates 423 ensures the overall strength of the stirring blade 42; the connecting plate 41 is connected to the reinforcement plate 423 located at the top, so that the driving motor 31 can drive the stirring blade 42 to rotate through the connecting plate 41.

[0050] The drying barrel 1 also includes an air jet unit 5, which includes a high-pressure pump 51 mounted on the top outer wall of the drying barrel 1. The high-pressure pump 51 is connected to a high-pressure nozzle 53 via a connecting pipe 52. The high-pressure nozzle 53 is provided with a plurality of high-pressure nozzles 54 facing the axis of the drying barrel 1. The inner wall of the drying barrel 1 is provided with a groove 14 for the high-pressure nozzle 53 to be embedded. When the crystals are dried, they will be discharged through the discharge port 12. At this time, the high-pressure pump 51 can ventilate the high-pressure nozzle 53 through the connecting pipe 52, and the high-pressure nozzle 53 will spray high-pressure gas into the heating chamber through the high-pressure nozzles 54. The stirring blade 42 will pass through the high-pressure nozzles 54 during its rotation, and the high-pressure gas will blow away the crystals attached to the stirring blade 42, so that these crystals can be discharged through the discharge port 12, thereby reducing the amount of crystals remaining in the drying barrel 1.

[0051] The end of the stirring blade 42 that is used to contact the inner wall of the drying barrel 1 and the end of the stirring blade 42 that is used to contact the outer wall of the material guide barrel 2 will pass through the high-pressure nozzle 54 in succession, so that the high-pressure gas can be sprayed to the end of the stirring blade 42 that is used to contact the inner wall of the drying barrel 1 and the end of the stirring blade 42 that is used to contact the outer wall of the material guide barrel 2 in succession, thereby improving the effect of blowing away the crystals attached to the stirring blade 42.

[0052] The material guide barrel 2 is provided with an air groove 24, which runs through the inner and outer walls of the material guide barrel 2, and the air groove 24 is directly opposite the high-pressure nozzle 54, so that the high-pressure gas ejected from the high-pressure nozzle can be sprayed onto the spiral blade 32 in the material guide barrel 2, so that the crystals attached to the spiral blade 32 can also be blown away, further reducing the residual crystals in the drying barrel 1.

[0053] Example 2

[0054] like Figures 5 to 7 As shown, it also includes a distribution barrel 6, the top of the distribution barrel 6 is connected to the feed hopper 61, and a distribution plate 62 is fixedly embedded in the distribution barrel 6 and located below the feed hopper 61. The distribution plate 62 is supported on the ground by a bracket 63, and the height of the upper surface of the distribution plate 62 gradually decreases from its center to its edge; the drying barrel 1 is provided with a total of multiple and is connected to the inside of the distribution barrel 6 through a material guide channel 64, one end of the material guide channel 64 is connected to the feed port 11, and the other end of the material guide channel 64 is located at the lowest point of the upper surface of the distribution plate 62.

[0055] When the crystals enter the distribution barrel 6 through the feed hopper 61, the crystals will fall to the upper surface of the distribution plate 62 and slide down along the upper surface of the distribution plate 62. The crystals will fall into each drying barrel 1 through the guide channel 64, realizing automatic distribution of the crystals. Multiple drying barrels 1 can dry the crystals together, improving the drying efficiency of the crystals.

[0056] It is worth noting that the inner wall of the distribution barrel 6, the upper surface of the distribution plate 62 and the inner wall of the material guide channel 64 are all coated with an anti-stick coating to minimize the adhesion of crystals.

[0057] like Figure 7 and Figure 8 As shown, the opening and closing unit 7 is further included. The opening and closing unit 7 includes a reduction motor 71 mounted on the bracket 63. The output shaft of the reduction motor 71 is connected to a plurality of opening and closing doors 73 via a connecting rod 72. The opening and closing doors 73 correspond one to one with the discharge port 12. The reduction motor 71 drives the opening and closing doors 73 to rotate via the connecting rod 72, so that the opening and closing doors 73 control the opening and closing of the discharge port 12.

[0058] A guide rail 74 is installed on the outer wall of the drying barrel 1, and one end of the opening and closing door 73 is rotatably embedded in the guide rail 74, so that the opening and closing door 73 remains stable during rotation and is not easy to bend, thereby improving the stability of the opening and closing door 73 when it is closed at the discharge port 12.

[0059] like Figure 5 As shown, a PLC controller 8 is installed on the outer wall of the distribution barrel 6. The PLC controller 8 has a loading button, a drying button, an unloading button and a reset button. The external feeding and conveying equipment, the heating wire 23, the drive motor 31, the high-pressure pump 51 and the reduction motor 71 are all coupled to the PLC controller 8.

[0060] The implementation principle of the histamine removal and extraction equipment of gentamicin sulfate in the embodiment of the present application is: during the drying process of the crystals, the worker first presses the loading button, and the PLC controller 8 will control the external feeding and conveying equipment to automatically convey the crystals to the feed hopper 61. The crystals will enter the distribution barrel 6 and fall to the upper surface of the distribution plate 62, and then the crystals will distribute the guide channel 64 to each drying barrel 1.

[0061] After the crystals are loaded, the worker will press the drying button, and the PLC controller 8 will control the heating wire and the drive motor 31 to start. The drive motor 31 will drive the spiral blade 32 and the stirring blade 42 to rotate. The stirring blade 42 will rotate and stir the crystals in the external heating chamber, and the spiral blade 32 will spirally transport the crystals in the internal heating chamber, so that the crystals are continuously turned and circulated in the external heating chamber and the internal heating chamber.

[0062] After the crystals are dried, the worker will press the unloading button, and the PLC controller 8 will control the reduction motor 71 to drive the opening and closing door 73 to rotate and disengage from the discharge port 12. The crystals in the drying barrel 1 will be discharged through the discharge port 12. The stirring blade 42 will rotate to scrape off the crystals on the wall of the outer drying chamber 13, and the spiral blade 32 will rotate to scrape off the crystals on the wall of the inner drying chamber 21. The high-pressure pump 51 will ventilate the high-pressure nozzle 53 through the connecting pipe 52, and the high-pressure nozzle 53 will spray high-pressure gas through the high-pressure nozzle 54, so that the crystals attached to the stirring blade 42 and the spiral blade 32 are blown away, and the scraped and blown crystals will be discharged through the discharge port 12.

[0063] After the crystals are discharged, the worker presses the reset button, and the PLC controller 8 controls the reduction motor 71 to drive the opening and closing door 73 to reclose the discharge port 12, and controls the heating wire, the drive motor 31 and the reduction motor 71 to be turned off.

[0064] In summary, the present application can continuously turn over the crystals during the crystal drying process and make the crystals perform a circular motion, and quickly clean up the remaining crystals after the crystals are dried, thereby improving the drying efficiency of the crystals.

[0065] Example 3

[0066] The present application discloses a device for removing histamine from gentamicin sulfate. A method for removing histamine from gentamicin sulfate comprises the following steps:

[0067] S1. Select gentamicin sulfate fermentation broth with a high histamine content (HPLC detection is recommended to confirm the histamine content is >500 ppm), remove bacterial residues and insoluble impurities by centrifugation to obtain a centrifuge at a speed of 4000 rpm for 15 min;

[0068] S2, using nanofiltration membrane (NF270, molecular weight cut-off 200Da) to concentrate the centrifuge, the operating pressure is 15bar, the temperature is 25 ℃ gentamicin sulfate (molecular weight 477Da) is retained, histamine (molecular weight 111Da) passes through the nanofiltration membrane with the centrifuge to obtain a separated liquid, achieving preliminary separation, and the resin adsorbing gentamicin is eluted with 0.5M NaCl to recover gentamicin and reduce losses;

[0069] S3. Use a weakly acidic cation exchange resin (such as Amberlite IRC86) to adsorb gentamicin sulfate (polyamino group, high charge density) in the separation solution at a pH of 6.0-6.5 at a flow rate of 1 BV / h. Histamine passes through the resin (histamine penetration rate > 90%) to obtain a permeate.

[0070] S4. Adjust the pH of the permeate to 10-11, adsorb histamine with a macroporous adsorption resin (such as HPD-100), elute with ethanol-water (1:1), and concentrate to obtain crude histamine;

[0071] S5. Add 1% activated carbon (w / v) to the crude histamine solution (5-10% concentration), stir for 30 minutes, and filter to remove pigments and trace organic matter to obtain a filtrate;

[0072] S6. Using a strongly acidic cation exchange resin (such as 001×7), the filtrate was gradiently eluted with 0.1 M NaOH to collect a high-purity histamine component to obtain a concentrate;

[0073] S7, adjusting the pH value of the concentrate to 3.0-3.5, cooling to 4°C for crystallization for 12 hours at a cooling rate of 0.5°C / min;

[0074] S6. The crystals are vacuum dried using a gentamicin sulfate histamine removal extraction device at a vacuum degree of -0.09 MPa and a temperature of 40°C.

[0075] The above are all preferred embodiments of the present application, and are not intended to limit the scope of protection of the present application. Therefore, any equivalent changes made based on the structure, shape, and principle of the present application should be included in the scope of protection of the present application.

Claims

1. A histamine removal and extraction device for gentamicin sulfate, characterized in that: The drying barrel (1) comprises a drying barrel (1) having a material inlet (11) and a material outlet (12), the drying barrel (1) being arranged vertically, a material guide barrel (2) being arranged inside the drying barrel (1), the material guide barrel (2) and the drying barrel (1) being arranged coaxially, and electric heating wires (23) being arranged between the inner wall and the outer wall of the drying barrel (1) and between the inner wall and the outer wall of the material guide barrel (2); An outer drying chamber (13) is provided between the outer wall of the material guide barrel (2) and the inner wall of the drying barrel (1); an inner drying chamber (21) is provided in the material guide barrel (2); the bottom of the outer drying chamber (13) is connected to the bottom of the inner drying chamber (21); and the top of the outer drying chamber (13) is connected to the top of the inner drying chamber (21); A spiral lifting unit (3) is provided in the inner drying chamber (21), and a rotary stirring unit (4) is provided in the outer drying chamber (13).

2. The histamine removal and extraction equipment of gentamicin sulfate according to claim 1, characterized in that: The spiral lifting unit (3) comprises a driving motor (31) arranged on the drying barrel (1); a spiral blade (32) is coaxially connected to the output shaft of the driving motor (31); and the spiral blade (32) is rotatably embedded in the inner drying chamber (21).

3. The histamine removal and extraction equipment of gentamicin sulfate according to claim 2, characterized in that: The rotary stirring unit (4) comprises a stirring blade (42) connected to the output shaft of the driving motor (31) via a connecting plate (41), and the stirring blade (42) contacts the inner wall of the drying barrel (1) and the outer wall of the material guide barrel (2).

4. The histamine removal and extraction equipment of gentamicin sulfate according to claim 3, characterized in that: The invention also includes an air jet unit (5), which includes a high-pressure pump (51). The high-pressure pump (51) is connected to a high-pressure nozzle (53) through a connecting pipe (52). The high-pressure nozzle (53) is provided with a plurality of high-pressure nozzles (54) facing the axis of the drying barrel (1). The inner wall of the drying barrel (1) is provided with a groove (14) for the high-pressure nozzle (53) to be embedded. The stirring blade (42) will pass through the high-pressure nozzle (54) during the rotation process.

5. The histamine removal and extraction equipment of gentamicin sulfate according to claim 4, characterized in that: The end of the stirring blade (42) that contacts the inner wall of the drying barrel (1) and the end of the stirring blade (42) that contacts the outer wall of the material guide barrel (2) will successively pass through the high-pressure nozzle (54).

6. The histamine removal and extraction equipment of gentamicin sulfate according to claim 5, characterized in that: The material guide barrel (2) is provided with an air groove (24), which penetrates the inner and outer walls of the material guide barrel (2), and the air groove (24) is directly opposite to the high-pressure nozzle (54).

7. The histamine removal and extraction equipment of gentamicin sulfate according to claim 1, characterized in that: The inner diameter of the lower part of the drying barrel (1) gradually decreases from top to bottom.

8. The histamine removal and extraction equipment for gentamicin sulfate according to any one of claims 1 to 7, characterized in that: The invention also includes a material distribution barrel (6), a material feed hopper (61) is provided on the material distribution barrel (6), a material distribution plate (62) is provided in the material distribution barrel (6) and is located below the material feed hopper (61), and the height of the upper surface of the material distribution plate (62) gradually decreases from the center to the edge thereof; The drying barrel (1) is provided with a plurality of guide channels (64) connected to the interior of the distribution barrel (6), one end of the guide channel (64) is connected to the feed port (11), and the other end of the guide channel (64) is located at the lowest point of the upper surface of the distribution plate (62).

9. The histamine removal and extraction equipment of gentamicin sulfate according to claim 8, characterized in that: The invention also includes an opening and closing unit (7), which includes a reduction motor (71). The output shaft of the reduction motor (71) is provided with a plurality of opening and closing doors (73) for controlling the opening and closing of the discharge port (12). The opening and closing doors (73) correspond to the discharge port (12) in a one-to-one manner.

10. A method for removing histamine from gentamicin sulfate, characterized in that: The following steps are included: S1. Selecting a gentamicin sulfate fermentation broth with a high histamine content, removing bacterial residues and insoluble impurities by centrifugation to obtain a centrifuge; S2, using a nanofiltration membrane to concentrate the centrifuge liquid, gentamicin sulfate is retained, and histamine passes through the nanofiltration membrane along with the centrifuge liquid to obtain a separated liquid; S3, using a weakly acidic cation exchange resin at pH 6.0-6.5 to adsorb gentamicin sulfate in the separation solution, and histamine passes through the resin to obtain a flowthrough; S4. Adjust the pH of the permeate to 10-11, adsorb histamine with a macroporous adsorption resin, and elute with ethanol-water (1:1). After concentration, obtain crude histamine; S5. Concentrate the crude histamine, adjust the pH to 3.0-3.5, and allow to stand at 4°C for crystallization; S6. Drying the crystals using the histamine removal and extraction equipment for gentamicin sulfate according to claim 8 or 9.

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