Composition for preventing and treating yellow disease of papaya and use method of composition

Abstract

The invention discloses a composition for preventing and treating papaya yellows and a use method thereof, and relates to the technical field of plant disease prevention and treatment. The composition for preventing and treating the yellow disease of the papaya is prepared from the following raw materials in parts by weight: 60 to 80 parts of plant extract, 10 to 15 parts of tetracycline hydrochloride, 15 to 22 parts of zymosan, 2 to 4 parts of jasmonic acid methyl ester, 2 to 4 parts of boric acid, 7 to 14 parts of animal protein amino acid, 3 to 6 parts of diethyl aminoethyl hexanoate, 4 to 9 parts of gamma-aminobutyric acid, 2 to 8 parts of proline, 3 to 7 parts of glycine and 5 to 10 parts of calcium chloride. When the composition is used, the composition and water are mixed to form liquid medicine, then the liquid medicine is injected into papaya plants through drill holes, and finally the drill holes are sealed and disinfected. The composition disclosed by the invention can effectively kill phytoplasma, improve the immunity of plants, enhance the growth vigor of the plants and prevent and treat the yellow disease of the papaya, and is high in prevention effect, quick in effect taking and long in pesticide effect.

Description

Technical Field

[0001] The present invention relates to the technical field of plant disease prevention and control, and in particular to a composition for preventing and controlling papaya chlorosis and a use method thereof. Background Art

[0002] Papaya (Carica papaya L.), taxonomically belonging to the Caricaceae family and the genus Carica, has considerable economic value. Papaya yellowing disease is caused by a phytoplasma that parasitizes the papaya's phloem. Infected papaya leaves turn yellow, gradually wither and die, and once infected, the plant can lose a significant amount of yield. Conventional fungicides are difficult to kill the pathogen. Current prevention and control of papaya yellowing disease primarily relies on strengthening field management, enhancing plant resistance, promptly removing diseased plants, and spraying with copper-containing fungicides and antibiotics. However, these measures are ineffective in preventing and controlling papaya yellowing disease, as they are ineffective in killing the pathogen and are prone to developing drug resistance. In addition, the trunk of the papaya tree is hollow and has a medullary cavity, so it can be sterilized by injecting drugs through the trunk, which has a good sterilization effect. However, the applicant found that the drug diffusion is slow when injecting, and the drug cannot be evenly distributed throughout the plant in a timely manner, which to some extent affects the control effect. Although the method of drilling multiple holes in the tree can evenly supply the drug, it increases the workload and cost.

[0003] Based on this, the purpose of this application is to provide a composition for preventing and treating papaya yellowing disease and a method of use thereof to solve the above problems. Summary of the Invention

[0004] To address the above shortcomings, the present invention provides a composition for preventing and treating papaya chlorosis and a method for using the composition. The composition can effectively kill phytoplasmas, improve plant immunity, enhance plant growth, and prevent and treat papaya chlorosis. The composition has high prevention efficacy, rapid effect, and long-lasting efficacy. The specific technical solution is as follows:

[0005] A composition for preventing and treating papaya yellowing disease comprises the following raw materials in parts by weight: 60-80 parts of plant extract, 10-15 parts of tetracycline hydrochloride, 15-22 parts of yeast polysaccharide, 2-4 parts of methyl jasmonate, 2-4 parts of boric acid, 7-14 parts of animal protein amino acids, 3-6 parts of diethylaminoethyl hexanoate, 4-9 parts of gamma-aminobutyric acid, 2-8 parts of proline, 3-7 parts of glycine and 5-10 parts of calcium chloride.

[0006] Preferably, the composition comprises the following raw materials in parts by weight: 65-78 parts of plant extract, 12-14 parts of tetracycline hydrochloride, 18-20 parts of yeast polysaccharide, 2-3 parts of methyl jasmonate, 3-4 parts of boric acid, 8-11 parts of animal protein amino acids, 4-5 parts of diethylaminoethyl hexanoate, 6-7 parts of γ-aminobutyric acid, 4-6 parts of proline, 4-5 parts of glycine and 7-8 parts of calcium chloride.

[0007] Preferably, the preparation method of the plant extract is: 25-35 parts of rosemary, 40-55 parts of thyme, 18-25 parts of cloves, 22-37 parts of garlic and 10-18 parts of myrtle are crushed together to obtain a mixture, 30-50 times the weight of water is added to the mixture, and the mixture is decocted for 1-2 hours, filtered, and the filter residue is decocted 2-3 times in the same manner, the decoctions are combined, and the decoctions are concentrated to 1 / 3 of the original volume to obtain the plant extract.

[0008] Preferably, the yeast polysaccharide preparation method comprises: placing cerevisiae yeast in an aqueous solution with a pH of 4-5 and subjecting it to acid treatment for 20-30 minutes, filtering, grinding the acid-treated yeast to obtain pretreated yeast, adding the pretreated yeast to water at 80-100° C. and stirring and extracting for 2-3 hours at a solid-liquid ratio of 1:50-70, filtering, collecting the filtrate, concentrating the filtrate to half of its original volume to obtain a concentrated solution, adding the concentrated solution to an ethanol solution with a mass concentration of 80-90% to an alcohol content of 80-85%, standing overnight, centrifuging, removing the supernatant, washing the precipitate 2-3 times, and freeze-drying to obtain the yeast polysaccharide.

[0009] Preferably, the animal protein amino acids are mainly composed of lysine and methionine, and the mass ratio of lysine to methionine is 1:1-7.

[0010] The present invention also provides a method for using the composition for preventing and treating papaya chlorosis, comprising the following steps:

[0011] (1) mixing the composition with water to prepare a liquid medicine;

[0012] (2) injecting the drug solution into the papaya plant through a drill hole;

[0013] (3) The drilled hole is sealed and disinfected.

[0014] Preferably, in step (1), the weight ratio of the composition to water is 1:600-800.

[0015] Preferably, in step (2), the drilling injection is specifically as follows: first, use an electric drill to drill a hole at the base of the stem of the diseased plant, the hole being 10-15 cm from the ground, and then injecting the liquid medicine into the medullary cavity of the diseased plant through a hanging bottle; in the early stage of papaya yellowing disease, the medicine is applied once every 5-8 days, the dosage is 100-150 mL / plant, and a total of 3-5 times; when the papaya is not diseased, spray or drip irrigation begins when the first buds appear, and the medicine is applied once every 20-40 days, the dosage is 100-150 mL / plant, and a total of 2-4 times.

[0016] Preferably, in step (3), the sealing and disinfection is performed by using calcium carbonate to seal and disinfect the drill hole.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] The composition of the present invention can effectively kill phytoplasmas, improve plant immunity, enhance plant growth, and prevent and control papaya yellowing disease, with high prevention efficiency, rapid effect, and long-lasting efficacy. The plant extract, tetracycline hydrochloride, and yeast polysaccharide can all effectively kill phytoplasmas, and the combined use of the three can reduce the resistance of phytoplasmas to tetracycline hydrochloride, reduce the amount of tetracycline hydrochloride used, and prolong the efficacy of the drug. The combined action of methyl jasmonate, boric acid, aminoethyl amine hexadecene, and animal protein amino acids can provide sufficient nutrition for the tree, promote photosynthesis and energy metabolism, promote plant growth, and thereby enhance disease resistance. The combined use of gamma-aminobutyric acid, proline, and glycine can increase the permeability of plant cell membranes, thereby improving the penetration performance of the drug, accelerating the conduction of the drug solution in the plant body, ensuring uniform distribution of the drug in the plant body and achieving rapid effect. The combined use of calcium chloride, boron, proline, and glycine can activate the enzymatic activities of multiple stress-resistant enzymes in the plant body, thereby improving the stress resistance of the plant. Therefore, the components in the composition of the present invention cooperate with each other to achieve a significant prevention and treatment effect of papaya yellowing disease. DETAILED DESCRIPTION

[0019] The specific embodiments of the present invention are described in detail below, but it should be understood that the protection scope of the present invention is not limited by the specific embodiments.

[0020] Example 1

[0021] A composition for preventing and treating papaya chlorosis comprises the following raw materials in parts by weight: 60 parts of plant extract, 10 parts of tetracycline hydrochloride, 15 parts of yeast polysaccharide, 2 parts of methyl jasmonate, 2 parts of boric acid, 7 parts of animal protein amino acids, 3 parts of diethylaminoethyl hexanoate, 4 parts of gamma-aminobutyric acid, 2 parts of proline, 3 parts of glycine and 5 parts of calcium chloride.

[0022] The preparation method of the plant extract comprises the following steps: crushing 25 parts of rosemary, 40 parts of thyme, 18 parts of cloves, 22 parts of garlic and 10 parts of myrtle together to obtain a mixture; adding 30 times the weight of water to the mixture, decocting for 1 hour, filtering, decocting the filter residue twice in the same manner, combining the decoctions, and concentrating the decoctions to 1 / 3 of the original volume to obtain the plant extract.

[0023] The yeast polysaccharide preparation method comprises the following steps: placing brewer's yeast in an aqueous solution with a pH of 4 and subjecting it to acid treatment for 20 minutes, filtering, grinding the acid-treated yeast to obtain pretreated yeast, adding the pretreated yeast to 80°C water and stirring for extraction for 2 hours at a solid-liquid ratio of 1:50, filtering, collecting the filtrate, concentrating the filtrate to half of its original volume to obtain a concentrated solution, adding the concentrated solution to an ethanol solution with a mass concentration of 80% until the alcohol content reaches 80%, allowing it to stand overnight, centrifuging, removing the supernatant, washing the precipitate twice, and freeze-drying to obtain the yeast polysaccharide.

[0024] The animal protein amino acids are mainly composed of lysine and methionine, and the mass ratio of lysine to methionine is 1:1.

[0025] Example 2

[0026] A composition for preventing and treating papaya chlorosis comprises the following raw materials in parts by weight: 64 parts of plant extract, 12 parts of tetracycline hydrochloride, 22 parts of yeast polysaccharide, 4 parts of methyl jasmonate, 4 parts of boric acid, 14 parts of animal protein amino acids, 6 parts of diethylaminoethyl hexanoate, 9 parts of gamma-aminobutyric acid, 8 parts of proline, 7 parts of glycine and 10 parts of calcium chloride.

[0027] The preparation method of the plant extract comprises the following steps: crushing 28 parts of rosemary, 46 parts of thyme, 22 parts of cloves, 35 parts of garlic and 116 parts of myrtle together to obtain a mixture; adding 40 times the weight of water to the mixture, decocting for 1.5 hours, filtering, decocting the filter residue twice in the same manner, combining the decoctions, and concentrating the decoctions to 1 / 3 of the original volume to obtain the plant extract.

[0028] The yeast polysaccharide preparation method comprises the following steps: placing brewer's yeast in an aqueous solution with a pH of 5 and subjecting it to acid treatment for 30 minutes, filtering, grinding the acid-treated yeast to obtain pretreated yeast, adding the pretreated yeast to 100° C. water and stirring for extraction for 3 hours at a solid-liquid ratio of 1:70, filtering, collecting the filtrate, concentrating the filtrate to half of its original volume to obtain a concentrated solution, adding the concentrated solution to an ethanol solution with a mass concentration of 90% to an alcohol content of 85%, standing overnight, centrifuging, removing the supernatant, washing the precipitate three times, and freeze-drying to obtain the yeast polysaccharide.

[0029] The animal protein amino acids are mainly composed of lysine and methionine, and the mass ratio of lysine to methionine is 1:7.

[0030] Example 3

[0031] A composition for preventing and treating papaya chlorosis comprises the following raw materials in parts by weight: 75 parts of plant extract, 13 parts of tetracycline hydrochloride, 19 parts of yeast polysaccharide, 3 parts of methyl jasmonate, 3 parts of boric acid, 10 parts of animal protein amino acids, 5 parts of diethylaminoethyl hexanoate, 6 parts of gamma-aminobutyric acid, 4 parts of proline, 5 parts of glycine and 8 parts of calcium chloride.

[0032] The preparation method of the plant extract comprises the following steps: crushing 30 parts of rosemary, 49 parts of thyme, 22 parts of cloves, 30 parts of garlic and 16 parts of myrtle together to obtain a mixture; adding 40 times the weight of water to the mixture, boiling for 2 hours, filtering, repeatedly boiling the filter residue in the same manner for 2-3 times, combining the decoctions, and concentrating the decoctions to 1 / 3 of the original volume to obtain the plant extract.

[0033] The yeast polysaccharide preparation method comprises the following steps: placing brewer's yeast in an aqueous solution with a pH of 4 and subjecting it to acid treatment for 25 minutes, filtering, grinding the acid-treated yeast to obtain pretreated yeast, adding the pretreated yeast to 90° C. water and stirring for extraction for 2.5 hours at a solid-liquid ratio of 1:60, filtering, collecting the filtrate, concentrating the filtrate to half of its original volume to obtain a concentrated solution, adding the concentrated solution to an ethanol solution with a mass concentration of 85% to an alcohol content of 82%, standing overnight, centrifuging, removing the supernatant, washing the precipitate twice, and freeze-drying to obtain the yeast polysaccharide.

[0034] The animal protein amino acids are mainly composed of lysine and methionine, and the mass ratio of lysine to methionine is 1:5.

[0035] Example 4

[0036] A composition for preventing and treating papaya chlorosis comprises the following raw materials in parts by weight: 78 parts of plant extract, 14 parts of tetracycline hydrochloride, 20 parts of yeast polysaccharide, 3 parts of methyl jasmonate, 3.5 parts of boric acid, 11 parts of animal protein amino acids, 5 parts of diethylaminoethyl hexanoate, 7 parts of gamma-aminobutyric acid, 6 parts of proline, 5 parts of glycine and 8 parts of calcium chloride.

[0037] The preparation method of the plant extract comprises the following steps: crushing 35 parts of rosemary, 55 parts of thyme, 25 parts of cloves, 37 parts of garlic and 18 parts of myrtle together to obtain a mixture; adding 50 times the weight of water to the mixture, boiling for 2 hours, filtering, repeatedly boiling the filter residue three times in the same manner, combining the decoctions, and concentrating the decoctions to 1 / 3 of the original volume to obtain the plant extract.

[0038] The yeast polysaccharide preparation method comprises the following steps: placing brewer's yeast in an aqueous solution with a pH of 4 and subjecting it to acid treatment for 20 minutes, filtering, grinding the acid-treated yeast to obtain pretreated yeast, adding the pretreated yeast to 85° C. water and stirring for extraction for 2 hours at a solid-liquid ratio of 1:65, filtering, collecting the filtrate, concentrating the filtrate to half of its original volume to obtain a concentrated solution, adding the concentrated solution to an ethanol solution with a mass concentration of 85% until the alcohol content reaches 80%, allowing the solution to stand overnight, centrifuging, removing the supernatant, washing the precipitate twice, and freeze-drying to obtain the yeast polysaccharide.

[0039] The animal protein amino acids are mainly composed of lysine and methionine, and the mass ratio of lysine to methionine is 1:6.

[0040] Example 5

[0041] A method for using the composition for preventing and treating papaya chlorosis comprises the following steps:

[0042] (1) The composition of Example 1 was mixed with water at a weight ratio of 1:600 ​​to prepare a liquid medicine;

[0043] (2) First, use an electric drill to drill a hole at the base of the stem of the diseased plant, the hole being 10 cm from the ground, and then inject the liquid medicine into the medullary cavity of the diseased plant through a hanging bottle; in the early stage of papaya yellowing disease, apply the medicine once every 5 days, with a dosage of 100 mL / plant, for a total of 5 applications; when the papaya is not diseased, spray or drip irrigation is started when the first buds appear, and apply the medicine once every 20 days, with a dosage of 100 mL / plant, for a total of 4 applications;

[0044] (3) Calcium carbonate is used to seal and disinfect the drilled hole.

[0045] Example 6

[0046] A method for using the composition for preventing and treating papaya chlorosis comprises the following steps:

[0047] (1) The composition of Example 2 was mixed with water at a weight ratio of 1:800 to prepare a liquid medicine;

[0048] (2) First, use an electric drill to drill a hole at the base of the stem of the diseased plant, the hole being 15 cm from the ground, and then inject the liquid medicine into the medullary cavity of the diseased plant through a hanging bottle; in the early stage of papaya yellowing disease, apply the medicine once every 8 days, with a dosage of 150 mL / plant, for a total of 3 applications; when the papaya is not diseased, spray or drip irrigation is started when the first buds appear, and apply the medicine once every 40 days, with a dosage of 150 mL / plant, for a total of 2 applications;

[0049] (3) Calcium carbonate is used to seal and disinfect the drilled hole.

[0050] Example 7

[0051] A method for using the composition for preventing and treating papaya chlorosis comprises the following steps:

[0052] (1) The composition of Example 3 was mixed with water at a weight ratio of 1:700 to prepare a liquid medicine;

[0053] (2) First, use an electric drill to drill a hole at the base of the stem of the diseased plant, the hole being 15 cm from the ground, and then inject the liquid medicine into the medullary cavity of the diseased plant through a hanging bottle; in the early stage of papaya yellowing disease, apply the medicine once every 6 days, with a dosage of 120 mL / plant, for a total of 4 applications; when the papaya is not diseased, spray or drip irrigation is started when the first buds appear, and apply the medicine once every 30 days, with a dosage of 120 mL / plant, for a total of 3 applications;

[0054] (3) Calcium carbonate is used to seal and disinfect the drilled hole.

[0055] Comparative Example 1: This comparative example provides a composition for preventing and treating papaya yellowing disease. The composition is the same as Example 1 in terms of components and proportions except that the preparation method of yeast polysaccharide is different from that of Example 1.

[0056] The preparation method of yeast polysaccharide in this comparative example is as follows: placing cerevisiae yeast in an aqueous solution with a pH of 4 and subjecting it to acid treatment for 20 minutes, filtering, grinding the acid-treated yeast to obtain pretreated yeast, adding the pretreated yeast to 80°C water and stirring for extraction for 2 hours at a solid-liquid ratio of 1:50, filtering, collecting the filtrate, concentrating the filtrate to half of its original volume to obtain a concentrate, adding the concentrate to an 80% ethanol solution to an alcohol content of 80%, standing overnight, centrifuging, removing the supernatant, washing the precipitate twice, and freeze-drying to obtain crude yeast polysaccharide, removing protein from the crude yeast polysaccharide by the Sevag method (chloroform-n-butanol mixed solvent), collecting the liquid, and dialyzing the collected liquid with a dialysis bag. Finally, adding the dialyzed liquid to the ethanol solution according to the above method and standing until a precipitate is precipitated, washing the precipitate, and drying to obtain yeast polysaccharide.

[0057] Comparative Example 2: This comparative example provides a composition for preventing and treating papaya yellowing disease. The composition does not contain yeast polysaccharide, and other components and proportions are the same as those in Example 1.

[0058] Comparative Example 3: This comparative example provides a composition for preventing and treating papaya chlorosis. The composition does not contain boric acid and diethylaminoethyl hexanoate, and other components and proportions are the same as those in Example 1.

[0059] Comparative Example 4: This comparative example provides a composition for preventing and treating papaya chlorosis. The composition does not contain γ-aminobutyric acid and proline, and the other components and proportions are the same as those in Example 1.

[0060] Comparative Example 5: This comparative example provides a composition for preventing and treating papaya chlorosis. The composition does not contain proline and glycine, and other components and proportions are the same as those in Example 1.

[0061] Comparative Example 6: This comparative example provides a composition for preventing and treating papaya yellowing disease. Except for not containing calcium chloride, the other components and proportions of the composition are the same as those in Example 1.

[0062] Experiment on the efficacy of drugs for preventing and controlling papaya yellowing disease:

[0063] Test agents: the compositions of Examples 1 to 4 and Comparative Examples 1 to 6.

[0064] Control agent: 20% thiophanate-methyl suspension concentrate, registration certificate PD20086024, Zhejiang Longwan Chemical Co., Ltd., commercially available;

[0065] Test method: The test set up 11 treatments and 1 blank control (water), with 3 replicates for each treatment and 2 trees for each replicate. The test site was the papaya planting base in Hengzhou City, Nanning City, Guangxi Zhuang Autonomous Region, and papaya trees with the same yellowing were selected in the base for testing. The diseased trees were respectively supplied with medicine on May 20, 2023. In the early stage of papaya yellowing disease, Examples 1 to 4 and Comparative Examples 1 to 6, the medicine was supplied once every 5 days, with a dosage of 100 mL / plant, and a total of 5 applications. The control agent (20% thiophanate-methyl suspension) was supplied once every 5 days in the early stage of papaya yellowing disease, with a dosage of 100 mL / plant, and a total of 5 applications. The method of administration is: first drill a hole at the base of the stem of the plant with an electric drill, the hole being 15 cm from the ground, and then injecting the diluted liquid into the medullary cavity of the plant through a hanging bottle, and finally sealing and disinfecting the drilled hole with calcium carbonate.

[0066] No other fungicides were applied during the experiment. Disease status in each group was investigated on the 15th, 30th, and 45th day after drug application. The disease status of each leaf in each group was graded, and the disease index and control efficacy were calculated using the grading criteria. The results are shown in Tables 1, 2, and 3. Disease index = ∑ (number of disease stages at each level × number of disease stages) / (total number of plants investigated × 6) × 100. Control efficacy (%) = (control disease index - treatment disease index) / control disease index × 100.

[0067] Preventive effect (%) = (control disease index - treatment disease index) / control disease index × 100

[0068] The grading standards are as follows:

[0069] Level 0: normal plants, green leaves;

[0070] Level 1: 1 to 2 leaves on each plant turn yellow;

[0071] Level 2: 3 to 5 leaves on each plant are yellow;

[0072] Level 3: The whole plant turns yellow or even dies.

[0073] Table 1 Field control efficacy test of each group on papaya yellowing disease on the 15th day of drug application

[0074]

[0075]

[0076] Table 2 Field efficacy test of each group on the 30th day of drug application for papaya yellowing disease

[0077] Test drug Disease Index Prevention effect (%) Example 1 7.9 82.68 Example 2 7.6 83.33 Example 3 7.1 84.43 Example 4 6.8 85.09 Comparative Example 1 17.5 61.62 Comparative Example 2 20.9 54.17 Comparative Example 3 18.9 58.55 Comparative Example 4 19.7 56.80 Comparative Example 5 19.3 57.68 Comparative Example 6 16.3 64.25 20% Thiophanate-copper suspension 22.7 50.22 Blank control 45.6 /

[0078] Table 3 Field control efficacy test of each group on the 45th day of drug application for papaya yellowing disease

[0079]

[0080]

[0081] From the data of the embodiments and control agents in Tables 1 to 3, it can be seen that, compared with the control agent, the prevention efficacy of the composition of the present invention can reach 57-59% on the 15th day of use, and the prevention efficacy can reach 82-85% on the 30th day of use, indicating that the composition of the present invention is fast-acting. On the 45th day of use, the prevention efficacy is basically 100%, and the disease is basically under control, indicating that the composition of the present invention has high prevention efficacy.

[0082] From the data of Example 1 and Comparative Example 1 in Tables 1 to 3, it can be seen that the preparation method of yeast polysaccharide affects the control effect, and the yeast polysaccharide obtained by the preparation method of the present invention has a higher control effect.

[0083] From the data of Example 1 and Comparative Example 2 in Tables 1 to 3, it can be seen that the combination of tetracycline hydrochloride and yeast polysaccharide in the composition of the present invention can reduce the drug resistance of phytoplasmas, prolong the drug effect, and make the prevention effect more significant.

[0084] From the data of Example 1 and Comparative Examples 3 to 6 in Tables 1 to 3, it can be seen that the coordinated action of methyl jasmonate, boric acid, diethylaminoethyl ester and animal protein amino acids in the composition of the present invention can provide sufficient nutrition for the tree, promote photosynthesis and energy metabolism, promote plant growth, and thus enhance disease resistance; the coordinated use of γ-aminobutyric acid, proline and glycine in the composition of the present invention can increase the permeability of plant cell membranes, thereby improving the permeability of the agent, accelerating the conduction of the drug solution in the plant body, and making the agent evenly distributed in the plant body with rapid effect; the coordinated use of calcium chloride, boron, proline and glycine in the composition of the present invention can activate the enzymatic activities of multiple stress-resistant enzymes in the plant body, thereby improving the stress resistance of the plant.

[0085] In summary, the components in the composition of the present invention cooperate with each other to achieve a significant prevention and treatment effect of papaya yellowing disease.

[0086] The foregoing descriptions of specific exemplary embodiments of the present invention are for purposes of illustration and description. These descriptions are not intended to limit the invention to the precise forms disclosed, and it is apparent that many variations and modifications are possible in light of the foregoing teachings. The exemplary embodiments have been selected and described for the purpose of explaining the specific principles of the invention and their practical application, thereby enabling those skilled in the art to realize and utilize a variety of exemplary embodiments of the invention and various options and modifications. The scope of the invention is intended to be defined by the claims and their equivalents.

Claims

1. A composition for preventing and treating papaya yellowing disease, characterized in that: The composition comprises the following raw materials in parts by weight: 60-80 parts of plant extract, 10-15 parts of tetracycline hydrochloride, 15-22 parts of yeast polysaccharide, 2-4 parts of methyl jasmonate, 2-4 parts of boric acid, 7-14 parts of animal protein amino acids, 3-6 parts of diethylaminoethyl hexanoate, 4-9 parts of gamma-aminobutyric acid, 2-8 parts of proline, 3-7 parts of glycine and 5-10 parts of calcium chloride.

2. A composition for preventing and treating papaya yellowing disease according to claim 1, characterized in that: The composition comprises the following raw materials in parts by weight: 65-78 parts of plant extract, 12-14 parts of tetracycline hydrochloride, 18-20 parts of yeast polysaccharide, 2-3 parts of methyl jasmonate, 3-4 parts of boric acid, 8-11 parts of animal protein amino acids, 4-5 parts of diethylaminoethyl hexanoate, 6-7 parts of gamma-aminobutyric acid, 4-6 parts of proline, 4-5 parts of glycine and 7-8 parts of calcium chloride.

3. A composition for preventing and treating papaya yellowing disease according to claim 1 or 2, characterized in that: The preparation method of the plant extract comprises the following steps: grinding 25-35 parts of rosemary, 40-55 parts of thyme, 18-25 parts of cloves, 22-37 parts of garlic and 10-18 parts of myrtle together to obtain a mixture; adding 30-50 times the weight of water to the mixture, decocting for 1-2 hours, filtering, repeatedly decocting the filter residue 2-3 times in the same manner, combining the decoctions, and concentrating the decoctions to 1 / 3 of the original volume to obtain the plant extract.

4. A composition for preventing and treating papaya yellowing disease according to claim 1 or 2, characterized in that: The yeast polysaccharide preparation method comprises the following steps: placing brewer's yeast in an aqueous solution with a pH of 4-5 and subjecting it to acid treatment for 20-30 minutes, filtering, grinding the acid-treated yeast to obtain pretreated yeast, adding the pretreated yeast to water at 80-100° C. and stirring for extraction for 2-3 hours at a solid-liquid ratio of 1:50-70, filtering, collecting the filtrate, concentrating the filtrate to half of its original volume to obtain a concentrated solution, adding the concentrated solution to an ethanol solution with a mass concentration of 80-90% to an alcohol content of 80-85%, standing overnight, centrifuging, removing the supernatant, washing the precipitate 2-3 times, and freeze-drying to obtain the yeast polysaccharide.

5. A composition for preventing and treating papaya yellowing disease according to claim 1 or 2, characterized in that: The animal protein amino acids are mainly composed of lysine and methionine, and the mass ratio of lysine to methionine is 1:1-7.

6. A method for using the composition for preventing and treating papaya chlorosis according to any one of claims 1 to 5, characterized in that: The following steps are involved: (1) mixing the composition with water to prepare a liquid medicine; (2) injecting the drug solution into the papaya plant through a drill hole; (3) The drilled hole is sealed and disinfected.

7. The method for using the composition for preventing and treating papaya yellowing according to claim 6, characterized in that: In step (1), the weight ratio of the composition to water is 1:600-800.

8. The method for using the composition for preventing and treating papaya chlorosis according to claim 6, wherein: In step (2), the drilling injection is specifically as follows: first, use an electric drill to drill a hole at the base of the stem of the diseased plant, the hole being 10-15 cm from the ground, and then injecting the liquid medicine into the medullary cavity of the diseased plant through a hanging bottle; in the early stage of papaya yellowing disease, the medicine is applied once every 5-8 days, the dosage is 100-150 mL / plant, and a total of 3-5 times; when the papaya is not diseased, spray or drip irrigation is started when the first buds appear, and the medicine is applied once every 20-40 days, the dosage is 100-150 mL / plant, and a total of 2-4 times.

9. The method for using the composition for preventing and treating papaya chlorosis according to claim 6, wherein: In step (3), the sealing and disinfection is to use calcium carbonate to seal and disinfect the drill hole.

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