Sow anti-stress compound and application thereof

Abstract

The invention provides a sow anti-stress compound and application thereof, and belongs to the technical field of veterinary traditional Chinese medicine nutritional preparations. The anti-stress compound comprises an acanthopanax senticosus extract, lyase and one or more nutritional supplements. The acanthopanax senticosus extract is prepared by performing enzymolysis on an acanthopanax senticosus raw material with compound enzyme, concentrating supernate, performing spray drying to obtain an aqueous extract, performing alcohol extraction on medicine residues, performing spray drying to obtain an alcohol extract, and mixing the aqueous extract and the alcohol extract. The anti-stress compound for the sows is designed around the sows in the perinatal period, the problems of pain points in the perinatal period such as long birth process, high stillbirth rate and slow weaning estrus are solved, after the compound is used for treatment, the birth process of the sows is shortened, the weaning weight of piglets is increased by 20% or above, and the compound has good industrial production and large-scale application value.

Description

Technical Field

[0001] The invention belongs to the technical field of veterinary traditional Chinese medicine nutritional preparations, and particularly relates to a sow anti-stress compound and application thereof. Background Art

[0002] Sows often experience stress during pregnancy and the peripartum period due to environmental, labor, and metabolic factors, leading to decreased immune function, reduced feed intake, and restricted growth and development. Studies have shown that supplementing with specific active ingredients from traditional Chinese medicine can enhance the body's stress resistance, while vitamins and amino acids can improve nutritional status. Lytic enzymes (including phage-derived lysozymes or specific cell wall-degrading enzymes) are natural antimicrobial factors that effectively cleave the cell walls of Gram-positive and Gram-negative bacteria, reducing the burden of harmful bacteria in feed and drinking water, maintaining intestinal microbial balance, and strengthening the host immune barrier.

[0003] Eleutherococcus senticosus, a plant of the Araliaceae family, primarily contains eleutheroside B, eleutheroside E, polysaccharides, flavonoids, and polyphenols. Modern pharmacological studies have shown that these components possess a variety of biological activities, including: ① Anti-fatigue effects: Animal experiments have shown that eleutheroside B (50 mg / kg) significantly prolonged the swimming time of mice (P < 0.01), demonstrating superior anti-fatigue effects compared to ginsenoside Rb1. ② Antioxidant effects: The in vitro scavenging rate of eleutheroside polysaccharides against DPPH free radicals reached 85.3% (1 mg / mL), surpassing that of vitamin C (80.6%). ③ Immunomodulatory effects: eleutheroside E significantly increased serum IL-2 and IFN-γ levels and enhanced T cell proliferation in mice (100 mg / kg, P < 0.05).

[0004] Active ingredients such as eleutherosides, polysaccharides, and flavonoids are primarily distributed within plant cell walls and protoplasts. Polysaccharides and glycosides are bound to the cell wall polysaccharide matrix, making them difficult to fully release by conventional ethanol reflux extraction. Furthermore, the ethanol reflux extraction process suffers from high energy consumption, long extraction times, and the degradation of active ingredients. Eleutherosides B and E exist as aglycones + glycosyl groups, relying on cell wall degradation and matrix loosening. Traditional enzymatic hydrolysis-assisted extraction of eleutheroside extracts relies on a single enzyme, limiting extraction efficiency. Patent CN112370474A discloses a method for the complex enzyme-assisted ultrasonic extraction of total flavonoids from eleutherosides. This method involves direct addition of ethanol to ultrasonic extraction after complex enzyme hydrolysis. The resulting ethanol extract is primarily composed of flavonoids (such as quercetin and hyperoside), while ignoring other core active ingredients in eleutherosides (such as polysaccharides). Furthermore, ethanol precipitation results in the loss of water-soluble polysaccharides, which are crucial for immune regulation. Consequently, this method yields a single-function extract that cannot meet the multi-target regulatory requirements required for stress resistance in sows (e.g., polysaccharide-enhanced immunity).

[0005] In summary, it is necessary to develop a low-cost Acanthopanax senticosus extract suitable for sow farming in this field, and the clinical effect of the combination of Acanthopanax senticosus extract and lytic enzyme for sow stress resistance needs to be studied. Summary of the Invention

[0006] After in-depth and meticulous testing, the present invention has successfully developed a sow anti-stress compound and its application. This compound features: 1. A feed-adapted process for extracting all active components of Acanthopanax senticosus through a multi-enzyme synergistic approach; 2. A multi-target synergistic mechanism utilizing Acanthopanax sendicosus extract, bacteriophage lytic enzymes, and nutrients in sow stress pathways; and 3. A premix production method specifically for periparturient sows. In summary, this invention, by focusing on three-dimensional innovations—process, formulation, and application—effectively differentiates itself from current products used in pig farms and possesses significant industrial application value.

[0007] The technical solutions of the present invention are as follows:

[0008] In a first aspect, the present invention provides an anti-stress complex for sows, comprising an Acanthopanax senticosus extract, a lytic enzyme, and one or more nutritional supplements.

[0009] The complex contains 0.05-0.5% of Acanthopanax senticosus extract and 0.50-0.5% of lytic enzyme.

[0010] Preferably, the complex contains 0.1-0.4% Acanthopanax senticosus extract and 0.15-0.3% lyase.

[0011] Preferably, the lytic enzyme is a bacteriophage lytic enzyme.

[0012] Preferably, the complex further contains complex vitamins, complex amino acids, mineral salts, and fillers.

[0013] Preferably, the complex vitamin is one or more of vitamin A, vitamin D3, vitamin E, vitamin B1, vitamin B2, vitamin B6, niacin, biotin, and pantothenic acid.

[0014] Preferably, the complex amino acid is one or more of methionine, lysine, and γ-aminobutyric acid.

[0015] Preferably, the mineral salt is zinc.

[0016] Preferably, the filler is anhydrous glucose.

[0017] Preferably, the sow anti-stress complex is composed of the following raw materials: Acanthopanax senticosus extract, lyase, vitamin A, vitamin D3, vitamin E, vitamin B1, vitamin B2, vitamin B6, niacin, biotin, pantothenic acid, methionine, lysine, γ-aminobutyric acid, zinc, and anhydrous glucose.

[0018] Preferably, the dosage of each raw material component in the sow anti-stress complex per 1000g is: Acanthopanax senticosus extract 0.5-5.0g, lyase 0.5-5.0g, vitamin A 2,600,000-8,000,000IU, vitamin D3800,000-8,000,000IU, vitamin E 3,000-30,000 IU, vitamin B1 500-2000mg, vitamin B2 500-3,000mg, vitamin B6 500-5,000mg , niacin 3,000-9,000mg, biotin 50-250mg, pantothenic acid 200-26,000mg, methionine 1.0-2.5%, lysine 0.5-5.0%, γ-aminobutyric acid 500-5,000mg, zinc 20-100g, anhydrous glucose added to 1000g.

[0019] More preferably, the dosage of each raw material component in the sow anti-stress complex per 1000g is: Acanthopanax senticosus extract 1.0-4.0g, lyase 1.5-3.0g, vitamin A 4,600,000-6,000,000IU, vitamin D3 1,500,000-6,000,000IU, vitamin E 4,000-10,000 IU, vitamin B1 800-1200mg, vitamin B2 1,000-2,500mg, vitamin B6 200-4,000mg , niacin 4,000-8,000mg, biotin 60-100mg, pantothenic acid 500-1,500mg, methionine 1.0-2.2%, lysine 1.5-4.5%, γ-aminobutyric acid 1,000-3,000mg, zinc 40-80g, anhydrous glucose added to 1000g.

[0020] Further preferably, the dosage of each raw material component in every 1000g of the sow anti-stress complex is: 3.0g of Acanthopanax senticosus extract, 2.0g of lyase, 5,500,000IU of vitamin A, 5,500,000IU of vitamin D3, 5,500,000IU of vitamin E, 1000mg of vitamin B1, 1,500mg of vitamin B2, 2,000mg of vitamin B6, 6,000mg of niacin, 80mg of biotin, 1,000mg of pantothenic acid, 1.6% of methionine, 2.6% of lysine, 2,000mg of γ-aminobutyric acid, 50g of zinc, and anhydrous glucose is added to 1000g.

[0021] Preferably, the vitamin D3 is 25-hydroxyvitamin D3.

[0022] Preferably, the sow anti-stress complex is prepared by mixing the raw material components, stirring them evenly and setting them aside.

[0023] In a second aspect of the present invention, a method for preparing an Acanthopanax senticosus extract is provided, wherein the Acanthopanax senticosus extract is extracted by enzymatic hydrolysis, and the preparation steps are as follows:

[0024] S1. Dried and chopped Acanthopanax senticosus rhizome powder was obtained; the Acanthopanax senticosus powder was mixed with pH 5.0 phosphate buffer at a solid-liquid ratio of 1:15, and a complex enzyme preparation was added. Enzymatic hydrolysis was carried out at 50°C and stirred at 200 rpm for 2 h.

[0025] S2. The supernatant obtained in S1 was concentrated under reduced pressure and low temperature to a relative density of 1.04, and spray-dried to obtain an aqueous extract;

[0026] S3. Add 70% ethanol to the residue obtained in S1 at a solid-liquid ratio of 1:10, and stir and extract for 1 h. Separate the solid and liquid by vacuum filtration, and collect the filtrate. Rotary evaporate the filtrate under reduced pressure to a solid content of 30%. Spray dry or freeze dry to obtain an alcohol extract.

[0027] S4. The aqueous extract obtained in S2 is mixed with the alcohol extract obtained in S3 to obtain an Acanthopanax senticosus extract.

[0028] Preferably, the complex enzyme in S1 is a complex of cellulase, xylanase and pectinase, and the total enzyme activity is 15,000 U / g.

[0029] Preferably, the ratio of cellulase, xylanase and pectinase in the complex enzyme in S1 is 2:1:1.

[0030] Preferably, the spray drying conditions in S3 are an inlet air temperature of 160°C and an outlet air temperature of 80°C; and the freeze drying conditions are pre-freezing at -40°C for 12 h and vacuum drying for 24 h.

[0031] The third aspect of the present invention provides the use of the anti-stress compound for sows in preparing feed for reducing the stress of sows during the peripartum period and improving the growth performance of piglets.

[0032] Preferably, the improvement of peripartum sow stress is manifested in improved reproductive performance, including shortened labor, reduced stillbirth rate, and improved lactation performance.

[0033] Preferably, improving the growth performance of piglets is manifested in a reduced incidence of diarrhea in piglets and an increased weaning survival rate.

[0034] The compound formula of the present invention is designed as follows:

[0035] Table 1 Formula design of anti-stress compound for sows

[0036] .

[0037] The mechanism by which the ingredients of the present invention prevent and control stress is as follows:

[0038] Synergistic mechanisms (such as Figure 1 Acanthopanax senticosus flavonoids exhibit synergistic antioxidant effects in the intestinal microenvironment. Furthermore, combined with phage lytic enzymes to scavenge ROS in situ, this approach overcomes the limitations of single-dose supplementation in regulating systemic stress in sows.

[0039] The present invention is based on the core therapeutic principles of veterinary medicine and nutrients, which enhance the body's homeostasis based on the stress principle, reduces potential pathogens, eliminates endotoxins, lowers cortisol, and enhances disease resistance, and has obvious clinical advantages.

[0040] Beneficial effects of the present invention:

[0041] 1. The enzymatic hydrolysis and extraction of Acanthopanax senticosus in the present invention utilizes low-temperature and mild conditions to avoid thermal degradation of glycosides and polysaccharides. The combined enzymes act synergistically to effectively destroy the cell wall cellulose, pectin, and β-glucan structures, releasing intracellular glycosides and polysaccharides, significantly improving extraction efficiency. Experimental results confirm that the enzymatic hydrolysis method of the present invention can increase the total extraction yield of Acanthopanax senticosus polysaccharides and Acanthopanax senticosus active ingredients. It is energy-efficient, environmentally friendly, and free of solvent residue. It can be mass-produced in a workshop without the need for specialized equipment such as ultrasound or microwaves.

[0042] The present invention can significantly reduce the number of harmful bacteria in feed and drinking water and maintain the balance of intestinal microecology through the triple synergistic effect of the antioxidant effect of the active ingredients of Acanthopanax senticosus, the nutritional supplementation of vitamins and amino acids, and the antibacterial protection of phage-derived lytic enzymes. It can also enhance the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the serum of sows, reduce the level of malondialdehyde (MDA), improve feed intake, and promote the growth of weaned piglets.

[0043] The present invention is specifically designed for peripartum sows to solve peripartum pain points such as long labor process, high stillbirth rate, and slow weaning and estrus. After treatment with the composition of the present invention, the sow's labor process is shortened, the weaning weight of the piglets is increased, and the antioxidant and immune indicators are comprehensively improved. It has good industrial production and large-scale application value. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Figure 1. Mechanisms of the present invention's ingredients in preventing and controlling stress. DETAILED DESCRIPTION

[0045] The following will further explain the above content in detail through specific implementation methods in the form of examples. However, this should not be understood as limiting the scope of the above subject matter to the following examples. All technologies implemented based on the content of the present invention are within the scope.

[0046] Example 1 An anti-stress compound for sows

[0047] Formula preparation: Based on the active ingredients, 1.0g of Acanthopanax senticosus extract, 1.5g of bacteriophage lytic enzyme, 4,600,000IU of vitamin A, 1,500,000IU of 25-hydroxyvitamin D3, 4,000IU of vitamin E, 800mg of vitamin B1, 1,000mg of vitamin B2, 200mg of vitamin B6, 4,000mg of niacin, 60mg of biotin, 500mg of pantothenic acid, 1.0% of methionine, 1.5% of lysine, 1,000mg of γ-aminobutyric acid, 40g of zinc, and anhydrous glucose to 1000g; mix the above raw materials, stir well, and set aside;

[0048] The preparation steps of the Acanthopanax senticosus extract are as follows:

[0049] S1. Prepare dried and chopped Acanthopanax rhizome powder; mix the Acanthopanax powder with pH 5.0 phosphate buffer at a solid-to-liquid ratio of 1:15. Add a complex enzyme preparation of fibrinolytic enzyme, xylanase, and pectinase (2:1:1 ratio; total enzyme activity 15,000 U / g). Incubate the mixture at 50°C and 200 rpm for 2 h.

[0050] S2. The supernatant obtained in S1 was concentrated under reduced pressure and low temperature to a relative density of 1.04, and then spray-dried (inlet air temperature 160°C, outlet air temperature 80°C) to obtain an aqueous extract;

[0051] S3. Add 70% ethanol to the residue obtained in S1 at a solid-liquid ratio of 1:10, and stir for 1 hour. Separate the solid and liquid using vacuum filtration, and collect the filtrate. Rotary evaporate the filtrate under reduced pressure to a solid content of 30%. Spray dry (inlet air temperature 160°C, outlet air temperature 80°C) or freeze dry (prefreeze at -40°C for 12 hours, vacuum dry for 24 hours) to obtain the alcohol extract.

[0052] S4. The aqueous extract obtained in S2 is mixed with the alcohol extract obtained in S3 to obtain an Acanthopanax senticosus extract.

[0053] Example 2 An anti-stress compound for sows

[0054] Formula preparation: based on the active ingredients, 4.0 g of Acanthopanax senticosus extract, 3.0 g of bacteriophage lytic enzyme, 6,000,000 IU of vitamin A, 36,000,000 IU of 25-hydroxyvitamin D, 10,000 IU of vitamin E, 1200 mg of vitamin B1, 2,500 mg of vitamin B2, 4,000 mg of vitamin B6, 8,000 mg of niacin, 100 mg of biotin, 1,500 mg of pantothenic acid, 2.2% of methionine, 4.5% of lysine, 3,000 mg of γ-aminobutyric acid, 80 g of zinc, and anhydrous glucose are added to 1000 g; the above raw materials are mixed, stirred and set aside; the preparation steps of the Acanthopanax senticosus extract are as described in Example 1.

[0055] Example 3 An anti-stress compound for sows

[0056] Formula preparation: based on the active ingredients, 3.0 g of Acanthopanax senticosus extract, 2.0 g of bacteriophage lytic enzyme, 5,500,000 IU of vitamin A, 5,500,000 IU of 25-hydroxyvitamin D3, 5,500,000 IU of vitamin E, 1000 mg of vitamin B1, 1,500 mg of vitamin B2, 2,000 mg of vitamin B6, 6,000 mg of niacin, 80 mg of biotin, 1,000 mg of pantothenic acid, 1.6% of methionine, 2.6% of lysine, 2,000 mg of γ-aminobutyric acid, 50 g of zinc, and anhydrous glucose are added to 1000 g; the above raw materials are mixed, stirred and set aside; the preparation steps of the Acanthopanax senticosus extract are as described in Example 1.

[0057] Example 4 An anti-stress compound for sows

[0058] Formula preparation: Based on the active ingredients, Acanthopanax senticosus extract 3.0g, Vitamin A 5,500,000IU, 25-hydroxyvitamin D35,500,000 IU, Vitamin E 5,500IU, Vitamin B1 1000mg, Vitamin B21,500mg, Vitamin B62,000mg, Niacin 6,000mg, Biotin 80mg, Pantothenic acid 1,000mg, Methionine 1.6%, Lysine 2.6%, γ-aminobutyric acid 2,000mg, Zinc 50g, Lactobacillus plantarum 2.0g (10 6 CFU / g), anhydrous glucose is added to 1000g; the above raw material components are mixed, stirred evenly and set aside; the preparation steps of the Acanthopanax senticosus extract are the same as Example 1.

[0059] Example 5 Clinical Effect Trial of Anti-Stress Complex

[0060] Periparturient Stress Syndrome (PSS) has become a key factor limiting the profitability of modern pig production. Sows experience dramatic physiological and metabolic changes and immune function fluctuations from late gestation to early lactation (usually from seven days before to seven days after farrowing), resulting in a state of high stress.

[0061] 1 Experimental Purpose

[0062] This study systematically evaluated a novel anti-stress compound from Ruipu Bio (hereinafter referred to as the "Anti-Stress Compound") in peripartum sows, evaluating its antioxidant capacity and stress physiology regulation, as well as its sustained impact on subsequent piglet production performance. The experimental design strictly adhered to GCP clinical veterinary research standards, revealing the product's mechanism of action through multi-index testing, providing a scientific basis for peripartum management in large-scale pig farms.

[0063] 2 Materials and Methods

[0064] 2.1 Experimental site: A large-scale pig farm in Hebei Province (with a basic sow inventory of 1,200).

[0065] 2.2 Experimental animals: One hundred pregnant sows of the Long × Large crossbreed from 2 to 4 parities were selected with similar parity, backfat (18 ± 1.5 mm), and due date and were divided into five groups.

[0066] 2.3 Group design: control group (basal diet) and experimental group 1 (basal diet + 0.2% premix feed of Example 1 of the present invention), experimental group 2 (basal diet + 0.2% premix feed of Example 2 of the present invention), experimental group 3 (basal diet + 0.1% premix feed of Example 3 of the present invention), and experimental group 4 (basal diet + 0.2% premix feed of Example 4 of the present invention), each with 20 sows, were fed for 30 days.

[0067] Usage period: late gestation (96 days) to weaning (21 days).

[0068] 2.4 Feeding and management

[0069] The temperature in the farrowing house is maintained at 22-24℃ and the relative humidity is 60-70%. The pigs are moved to the farrowing house 7 days before farrowing to adapt to the environment and have free access to drinking water. They are fed 2.5kg / day during gestation and free access to food during lactation.

[0070] 2.5 Detection indicators

[0071] (1) Sows’ antioxidant capacity and anti-stress indicators

[0072] On the day of farrowing and the day of weaning, blood was collected from all pigs to measure serum superoxide dismutase (SOD) activity, serum catalase (CAT) activity, malondialdehyde (MDA) content, serum cortisol (COR) concentration, and serum adrenocorticotropic hormone (ACTH) content.

[0073] (2) Reproductive performance of sows: Detailed records of each pig’s delivery duration, number of stillbirths, litter weight at birth, litter weight at weaning, and weaning-estrus interval, etc.

[0074] (3) Piglet health: record the diarrhea rate (%), average daily weight gain (g), mortality rate, and weaning weight of each litter.

[0075] 2.5 Data Analysis

[0076] The data were expressed as mean ± standard deviation and analyzed by one-way analysis of variance (ANOVA) using SPSS 22.0. The differences among the groups were analyzed using Duncan's multiple comparison test, and P < 0.05 was considered significant.

[0077] 3 Test results

[0078] 3.1 Antioxidant and anti-stress capabilities of sows

[0079] Table 2 Serum indexes of sows on the day of farrowing and weaning (n=20)

[0080] .

[0081] (*P<0.05, **P<0.01).

[0082] Analysis: SOD activity decreased on the day of farrowing, but remained at higher levels in experimental groups 2 and 3 (P<0.01) and remained significantly higher than the control during the weaning recovery period. MDA, an oxidative product, increased significantly in the control group on the day of farrowing, but decreased significantly in experimental groups 2 and 3 (P<0.01). COR levels reached their peripartum peak on the day of farrowing, but were significantly suppressed in experimental groups 2 and 3 (P<0.01). COR levels decreased on the day of weaning, but remained significantly higher in experimental groups 2 and 3 than in the control group. ACTH levels fluctuated with the peripartum period, with the control group experiencing a significantly higher peak during the peripartum period than in all experimental groups. This suggests that the anti-stress complex can effectively enhance the peroxide scavenging capacity of peripartum sows and reduce oxidative stress.

[0083] Results: The antioxidant preparations in each experimental group were superior to those in the control group. The improvement effects of experimental groups 2 and 3 on antioxidant indicators SOD, MDA, and COR were significant (P<0.01). The determination of the content of anti-stress complexes in different groups showed that the embodiment can effectively inhibit the excessive stress response of peripartum sows and stabilize the function of the HPA axis.

[0084] 3.2 Sow reproductive performance records

[0085] The reproductive performance data of each group were recorded at the time of delivery and on the day of birth.

[0086] Table 3 Comparison of sow reproductive performance indicators (mean ± SD)

[0087] .

[0088] Note: * indicates significant difference compared with the control group (P<0.05).

[0089] The experimental sows showed improved overall reproductive performance, as follows:

[0090] All four experimental groups were better than the blank control group. Among them, the labor process of experimental groups 1, 2 and 3 was significantly shortened, the stillbirth rate was significantly reduced, and the daily milk production was significantly increased compared with the blank control group; the effect of experimental group 4 was not as good as that of experimental case 3.

[0091] 3.3 Piglet health and growth performance

[0092] After using the sow anti-stress product, piglets are significantly easier to raise, and the specific indicators are as follows.

[0093] Table 4 Piglet growth indicators and health status (0-21 days old)*

[0094] .

[0095] The four experimental groups were superior to the blank control group in terms of piglet growth indicators and health status, and the piglets achieved significant health improvement: compared with the blank control group, the diarrhea rate of experimental groups 1, 2 and 3 was reduced, the average daily weight gain was increased, and the weaning survival rate was improved, and all reached a significant difference level; the effect of experimental group 4 was not as good as that of experimental case 3.

[0096] 4 Discussion and Analysis

[0097] 4.1 Benefit Summary

[0098] Take the product of Example 1 as an example:

[0099] Improved reproductive performance: The product of the present invention can significantly shorten the delivery process, reduce the stillbirth rate, and extend the production life of sows, and has outstanding anti-stress effect on sows in the heavy pregnancy period.

[0100] Piglet health protection: Through the delivery of immune active substances through colostrum, the diarrhea rate of piglets is reduced by 50% (compared to the control group), and the weaning survival rate reaches 95.7%.

[0101] High input-output ratio: Assuming the feed additive cost is 120 yuan per ton, the profit of weaned piglets can be increased by 230 yuan per head (based on the current pig price of 14.26 yuan per kg).

[0102] 4.2 Production Application Recommendations

[0103] Precise addition plan: It is recommended to add 0.2% of the mixed feed in the late pregnancy (85 days to delivery), focusing on alleviating delivery stress.

[0104] Lactation period (parturition-weaning): Add 0.2% to mixed feed to ensure enzyme activity and continuously increase milk production.

[0105] In conjunction with the farm's mature environmental management, we focus on weak piglets at birth and targeted management to continuously improve their health status while avoiding possible disease transmission to ensure the health and production efficiency of the entire pig herd.

[0106] Example 6 Comparison of extraction effects of different methods

[0107] Comparison of extraction efficiency data of samples (Acanthopanax senticosus extract): Acanthopanax senticosus extract was extracted using the extraction method of Example 1 of the present invention and the method of Example 1 of patent CN112370474A (Comparative Example 1), respectively. The polysaccharide and eleutheroside contents and the total extraction rate of the active ingredients in the extract were measured, and the extraction rate was calculated. The comparative data are as follows.

[0108] Table 5 Data comparison and analysis

[0109] .

[0110] Comparative results: The overall extraction rate of the active ingredients of the present invention is about 35.4% (combined with the mixture of water extract and alcohol extract). The polysaccharide extraction rate of the enzymatic hydrolysis method of the present invention is 2.1 times higher than that of the hot reflux method in comparative example 1, which can significantly improve the extraction efficiency.

[0111] The extraction rate of eleutheroside by the enzymatic hydrolysis method of the present invention is similar to that of comparative example 1.

[0112] Taking into account the superposition of other components, the overall extraction efficiency of the two methods is that of the enzymatic hydrolysis method of the present invention is higher than that of comparative example 1, indicating that the enzymatic hydrolysis method has higher efficiency in the extraction process.

[0113] Conclusion: The enzymatic hydrolysis method employed in this invention significantly improves the extraction efficiency of polysaccharides through the synergistic effect of a complex enzyme preparation. Compared with the traditional hot reflux solvent extraction method, the overall extraction efficiency of all active ingredients was significantly improved. Therefore, the method not only improves the extraction rate but is also suitable for large-scale industrial production, showing high practical value.

[0114] Although the specific embodiments of the present invention are described above, those skilled in the art should understand that the specific embodiments described are merely illustrative and are not intended to limit the scope of the present invention. Equivalent modifications and changes made by those skilled in the art in accordance with the spirit of the present invention should be included within the scope of protection of the claims of the present invention.

Claims

1. A sow anti-stress compound, characterized in that: The complex comprises Acanthopanax senticosus extract, lytic enzyme and one or more nutritional supplements.

2. The anti-stress compound according to claim 1, characterized in that The complex contains 0.05-0.5% of Acanthopanax senticosus extract and 0.05-0.5% of lytic enzyme.

3. The anti-stress compound according to claim 1 or 2, characterized in that The Acanthopanax senticosus extract is extracted by enzymatic hydrolysis, and its preparation steps are as follows: S1. Dried and chopped Acanthopanax senticosus rhizome powder was obtained; the Acanthopanax senticosus powder was mixed with pH 5.0 phosphate buffer at a solid-liquid ratio of 1:15, and a complex enzyme preparation was added. Enzymatic hydrolysis was carried out at 50°C and stirred at 200 rpm for 2 h. S2. The supernatant obtained in S1 was concentrated under reduced pressure and low temperature to a relative density of 1.04, and spray-dried to obtain an aqueous extract; S3. Add 70% ethanol to the residue obtained in S1 at a solid-liquid ratio of 1:10, and stir and extract for 1 h. Separate the solid and liquid by vacuum filtration, and collect the filtrate. Rotary evaporate the filtrate under reduced pressure to a solid content of 30%. Spray dry or freeze dry to obtain an alcohol extract. S4. The aqueous extract obtained in S2 is mixed with the alcohol extract obtained in S3 to obtain an Acanthopanax senticosus extract.

4. The anti-stress complex according to claim 3, characterized in that The complex enzyme in S1 is a complex of cellulase, xylanase and pectinase, wherein the dosage ratio of cellulase, xylanase and pectinase in the complex enzyme is 2:1:1; the total enzyme activity is 15,000 U / g.

5. The anti-stress complex according to claim 3, characterized in that The spray drying conditions in S3 are an inlet air temperature of 160°C and an outlet air temperature of 80°C; the freeze drying conditions are pre-freezing at -40°C for 12 h and vacuum drying for 24 h.

6. The anti-stress complex according to claim 1 or 2, characterized in that The complex contains 0.1-0.4% of Acanthopanax senticosus extract and 0.15-0.3% of lytic enzyme; the lytic enzyme is bacteriophage lytic enzyme; the complex also contains complex vitamins, complex amino acids, mineral salts and fillers.

7. The anti-stress complex according to claim 6, characterized in that The dosage of each raw material component in each 1000g of the complex is as follows: 0.5-5.0g of Acanthopanax senticosus extract, 0.5-5.0g of lyase, 2,600,000-8,000,000IU of vitamin A, 800,000-8,000,000IU of vitamin D3, 3,000-30,000IU of vitamin E, 500-2000mg of vitamin B1, 500-3,000mg of vitamin B2, 500-5,000mg of vitamin B6, 3,000-9,000mg of niacin, 50-250mg of biotin, 200-26,000mg of pantothenic acid, 1.0-2.5% of methionine, 0.5-5.0% of lysine, 500-5,000mg of gamma-aminobutyric acid, 20-100g of zinc, and anhydrous glucose is added to 1000g.

8. The anti-stress complex according to claim 7, characterized in that The dosage of each raw material component in each 1000g of the complex is: Acanthopanax senticosus extract 1.0-4.0g, lyase 1.5-3.0g, vitamin A 4,600,000-6,000,000IU, vitamin D3 1,500,000-6,000,000IU, vitamin E 4,000-10,000IU, vitamin B1 800-1200mg, vitamin B2 1,000-2,500mg, vitamin B6 200-4,000mg , niacin 4,000-8,000mg, biotin 60-100mg, pantothenic acid 500-1,500mg, methionine 1.0-2.2%, lysine 1.5-4.5%, γ-aminobutyric acid 1,000-3,000mg, zinc 40-80g, anhydrous glucose added to 1000g.

9. The anti-stress complex according to claim 8, characterized in that The dosage of each raw material component in every 1000g of the complex is as follows: 3.0g of Acanthopanax senticosus extract, 2.0g of lyase, 5,500,000IU of vitamin A, 5,500,000IU of vitamin D3, 5,500,000IU of vitamin E, 1000mg of vitamin B1, 1,500mg of vitamin B2, 2,000mg of vitamin B6, 6,000mg of niacin, 80mg of biotin, 1,000mg of pantothenic acid, 1.6% of methionine, 2.6% of lysine, 2,000mg of gamma-aminobutyric acid, 50g of zinc, and anhydrous glucose is added to 1000g; the vitamin D3 is 25-hydroxyvitamin D3.

10. Use of the anti-stress compound for sows according to any one of claims 1 to 9 in preparing feed for reducing the stress of sows during the peripartum period and improving the growth performance of piglets.

Images