Two-stage low-soybean-meal daily ration for partridge chickens with green feet, preparation method and application of two-stage low-soybean-meal daily ration

Abstract

The invention relates to the technical field of feeding of partridge chickens with green feet, and discloses a two-stage low-soybean-meal daily ration for partridge chickens with green feet as well as a preparation method and application of the two-stage low-soybean-meal daily ration for partridge chickens with green feet. Wherein the content of soybean meal in the daily ration of 1-21d is 8-10%; the protein content is 18-20%; the content of amino acids in the crystal is as follows: 0.9%-1.2% of lysine, 0.45%-0.48% of methionine, 0.68%-0.72% of threonine and 0.24%-0.32% of tryptophan; the content of soybean meal in the daily ration at 21-100 days is 6-8%; the protein content is 16-19%; the amino acid content of the crystal is as follows: the content of lysine is 0.8 to 1.1 percent, the content of methionine is 0.36 to 0.40 percent, the content of threonine is 0.60 to 0.70 percent, and the content of tryptophan is 0.23 to 0.26 percent. The feed is suitable for feeding the partridge chickens, and the production performance of the partridge chickens is not affected.

Description

Technical Field

[0001] The invention relates to the technical field of breeding green-legged hens, and in particular to a two-stage low-soybean meal diet for green-legged hens, a preparation method and application thereof. Background Art

[0002] The intensive and large-scale development of animal husbandry has also led to an annual increase in the demand for corn-soybean meal-based compound feed.

[0003] Soybean meal, an agricultural byproduct obtained by extracting soybean oil, is an excellent source of protein feed due to its rich protein content, well-balanced amino acid profile, low anti-nutritional factors, and ease of digestion. Due to the high proportion of soybean meal in feed, there is a long-term positive correlation between feed grain prices and soybean meal prices. The rise and fluctuation of soybean meal raw material prices has led to increasing costs for fattening livestock, significantly increasing costs and risks in the livestock industry, impacting production efficiency and severely hindering the healthy development of the livestock breeding industry. Therefore, there is an urgent need to fully develop and utilize existing feed resources, rationally formulate multiple resources, and achieve a reasonable reduction and substitution of soybean meal in feed.

[0004] Currently, there are various feed formulation options for reducing soybean meal as an alternative, such as using straw, cake meal, dregs, and some forestry byproducts. However, there are also some issues, such as unbalanced nutritional profiles and high processing costs. Furthermore, existing chicken feeds are mostly focused on fast-growing, large-scale white-feathered broilers, lacking a dedicated low-soybean meal formula for green-legged broilers. Green-legged broilers have different requirements for amino acid balance and fiber tolerance due to their slow growth rate and high meat quality. Summary of the Invention

[0005] Purpose of the invention: In response to the problems existing in the prior art, the present invention provides a two-stage low-soybean meal diet for green-legged chickens, a preparation method and application. According to the physiological characteristics and nutritional requirements of green-legged chickens from 0 to 21 days of age (brooding period) and from 22 days of age to market (fattening period), low-soybean meal diet formulas are designed respectively. Multi-technology collaborative innovation is adopted to integrate the triple technology of microbial fermentation + enzyme preparation + ideal amino acid model to solve the limitations of single technology in replacing soybean meal.

[0006] Technical solution: In the first aspect, the present invention provides a two-stage low soybean meal diet for green-legged chickens, comprising a low soybean meal diet for the 1-21d stage and a low soybean meal diet for the 21-100d stage; wherein,

[0007] The low soybean meal diet for the 1-21 day period includes: soybean meal content of 8-10%; protein content of 18-20%; crystalline amino acid content of: lysine 0.9-1.2%, methionine 0.45-0.48%, threonine 0.68-0.72%, and tryptophan 0.24-0.32%;

[0008] The low soybean meal diet in the 21-100 d stage has the following contents: soybean meal content of 6-8%; protein content of 16-19%; crystalline amino acid content of lysine 0.8-1.1%, methionine 0.36-0.40%, threonine 0.60-0.70% and tryptophan 0.23-0.26%.

[0009] Furthermore, the low soybean meal diet in the 1-21d stage includes, by weight, 540-560 parts of corn, 90-110 parts of corn starch residue, 90-100 parts of soybean meal, 40-60 parts of rice bran, 50-70 parts of corn gluten meal, 40-50 parts of corn distiller's grains DDGS, 45-55 parts of cottonseed meal, 7.0-8.0 parts of soybean oil, 8.0-9.0 parts of L-lysine, 1.4-1.8 parts of DL-methionine, 13-14 parts of stone powder, 8-9 parts of calcium hydrogen phosphate, 0.8-1.2 parts of choline chloride, 0.2-0.4 parts of enzyme preparation, 3.0-3.4 parts of sodium chloride, and 3.6-4.0 parts of premix.

[0010] Furthermore, 500-510 parts of corn, 145-155 parts of corn starch residue, 60-80 parts of soybean meal, 30-35 parts of rice bran, 30-40 parts of corn gluten meal, 75-85 parts of corn distiller's grains DDGS, 45-55 parts of cottonseed meal, 35-45 parts of soybean oil, 8.0-8.5 parts of L-lysine, 0.8-1.0 part of DL-methionine, 13-14 parts of stone powder, 8.0-8.5 parts of calcium hydrogen phosphate, 0.6-1 part of choline chloride, 0.2-0.4 part of enzyme preparation, 3.0-3.5 parts of sodium chloride, and 3.0-4.0 parts of premix.

[0011] Furthermore, the enzyme preparation comprises acidic protease ≥50,000 U / g; xylanase ≥10,000 U / g; β-mannanase ≥3,000 U / g; cellulase ≥500 U / g; pectinase ≥1,000 U / g; phytase ≥500 U / g; and α-galactosidase ≥200 U / g.

[0012] Furthermore, the premix comprises, by weight, 2.5-3.5 parts of a mineral additive premix and 0.5-1.0 parts of a vitamin additive premix.

[0013] Furthermore, the mineral additive premix includes Fe 60.00-100.00 mg; Cu 6.00-10.00 mg; Mn 90.00-110.00 mg; Zn 70.00-90.00 mg; I 0.60-0.80 mg; and Se 0.20-0.40 mg.

[0014] Furthermore, the vitamin additive premix includes VA7000-9000 IU; VD3800-1200 IU; VE15-25 IU; VK30.40-0.60 mg; VB11.00-3.00 mg; VB26.00-10.00 mg; VB63.00-4.00 mg; VB 12 8.00-12.00μg; niacin 30.00-40.00mg; calcium pantothenate 8.00-12.00mg; folic acid 0.50-0.60mg; biotin 0.15-0.25mg.

[0015] In a second aspect, the present invention provides a method for preparing a two-stage low soybean meal diet for green-legged chickens as described in any one of the above items, the specific steps being as follows:

[0016] (1) Grinding corn, soybean meal, and cottonseed meal to obtain crushed materials; wherein the particle size of the crushed materials of the low soybean meal diet in the 1-21 day stage is 0.8-1.2 mm; the particle size of the crushed materials of the low soybean meal diet in the 21-100 day stage is 1.2-1.5 mm;

[0017] (2) weighing the raw materials according to the formula ratio, mixing the crushed material, the carrier and the premix to obtain a mixed material;

[0018] (3) The mixed material is tempered, pelletized, cooled, and then sprayed with enzyme preparations and oil to obtain a low soybean meal diet.

[0019] Furthermore, in step (1), the cottonseed meal is fermented with a composite probiotic: the composite probiotic combination is: Candida tropicalis 1×10 8 CFU / g cottonseed meal, Lactobacillus plantarum 5×10 7 CFU / g cotton meal, Bacillus subtilis 5×10 7 CFU / g cottonseed meal, Aspergillus niger 1×10 6 CFU / g cottonseed meal;

[0020] The specific fermentation process is: crush the cottonseed meal and mix it with water in a mass ratio of 1:0.5, then add 1% molasses, mix well, and ferment in bags for 72 hours. The temperature is controlled at 25-30°C; the fermentation is terminated when the pH drops below 4.5.

[0021] In a third aspect, the present invention provides a use of a two-stage low soybean meal diet for Qingjiao Ma chicken as described in any one of the above items in Qingjiao Ma chicken breeding.

[0022] Beneficial effects: Compared with the prior art, the present invention has the following specific beneficial effects:

[0023] The special low-soybean meal formula for the Qingjiao Ma chicken of the present invention adopts domestic regional specialty protein resources (such as domestic rice bran meal and corn germ meal) to replace soybean meal, and combines the fermentation process to reduce the dependence on imported soybean meal. The present invention designs low-soybean meal diet formulas respectively according to the physiological characteristics and nutritional requirements of Qingjiao Ma chickens from 0 to 21 days of age (brooding period) and 22 days of age to market (fattening period), breaking through the traditional "one-size-fits-all" formula model. Adopt multi-technological collaborative innovation: the composite probiotic fermentation technology is used to treat miscellaneous meal (such as cottonseed meal and rapeseed meal) to reduce anti-nutritional factors and improve protein utilization. In combination with low-protein diet technology, crystalline amino acids (lysine, methionine, tryptophan, threonine) are supplemented to balance nutrition to achieve the ideal amino acid model. Enzyme preparations are used to enhance the animal's decomposition of anti-nutritional factors and the digestion, absorption and utilization rate of nutrients. It integrates the triple technology of microbial fermentation + enzyme preparation + ideal amino acid model to solve the limitations of a single technology to replace soybean meal. This two-stage, low-soybean meal, low-protein, and amino acid-balanced diet is fully suitable for raising Qingjiao Ma chickens without compromising their performance. The soybean meal dosage is reduced from the conventional 14.5% to below 10%, reducing feed costs by 30 yuan per ton and achieving a difference in finished chicken weight of less than 2%. The formula is compatible with existing feed production line equipment, eliminating the need for additional investment. DETAILED DESCRIPTION

[0024] The present invention is described in detail below with reference to the embodiments.

[0025] Implementation method 1:

[0026] This embodiment provides a two-stage low soybean meal diet for green-legged chickens, the specific formula of which is as follows:

[0027] 1-21d low soybean meal diet formula: Each ton of feed contains 548kg of corn, 100kg of corn starch residue, 95kg of soybean meal, 50kg of rice bran, 60kg of corn gluten meal, 47kg of corn distillers grains, 50kg of cottonseed meal, 7.7kg of soybean oil, 8.6kg of lysine, 1.6kg of methionine, 13.5kg of stone powder, 8.6kg of calcium hydrogen phosphate, 0.9kg of choline chloride, 0.3kg of enzyme preparation, 3.2kg of salt, and 3.8kg of premix.

[0028] 21-100d low soybean meal diet formula: Each ton of feed contains 504kg of corn, 150kg of corn starch residue, 70kg of soybean meal, 32kg of rice bran, 35kg of corn gluten meal, 80kg of corn distillers grains, 50kg of cottonseed meal, 40kg of soybean oil, 8.2kg of lysine, 0.9kg of methionine, 13.5kg of stone powder, 8.2kg of calcium hydrogen phosphate, 0.8kg of choline chloride, 0.3kg of enzyme preparation, 3.2kg of salt, and 3.8kg of premix.

[0029] Among them, in the above enzyme preparation, acid protease ≥ 50,000U / g; xylanase ≥ 10,000U / g; β-mannanase ≥ 3,000U / g; cellulase ≥ 500U / g; pectinase ≥ 1,000U / g; phytase ≥ 500U / g; α-galactosidase ≥ 200U / g;

[0030] The premix comprises Fe 60.00-100.00 mg; Cu 6.00-10.00 mg; Mn 90.00-110.00 mg; Zn 70.00-90.00 mg; I 0.60-0.80 mg; Se 0.20-0.40 mg; VA 7000-9000 IU; VD 3800-1200 IU; VE 15-25 IU; VK 30.40-0.60 mg; VB 11.00-3.00 mg; VB 26.00-10.00 mg; VB 63.00-4.00 mg; VB 12 8.00-12.00μg; niacin 30.00-40.00mg; calcium pantothenate 8.00-12.00mg; folic acid 0.50-0.60mg; biotin 0.15-0.25mg.

[0031] The specific preparation method of the two-stage low soybean meal diet for green-legged chickens is as follows:

[0032] 1. Raw material reception and pretreatment: Detect moisture, mildew, impurities, etc. to ensure compliance with standards. Cleaning and impurity removal: Remove stones, metals and other foreign matter through screening and magnetic separation. Grinding: Grind corn, soybean meal, cottonseed meal, etc. to an appropriate particle size (1-21d low soybean meal diet: 0.8-1.2mm; 21-100d low soybean meal diet: 1.2-1.5mm). Among them, cottonseed meal is fermented with compound probiotics: the compound probiotic combination is: tropical Candida 1×10 8 CFU / g cottonseed meal, Lactobacillus plantarum 5×10 7 CFU / g cotton meal, Bacillus subtilis 5×10 7 CFU / g cottonseed meal, Aspergillus niger 1×10 6CFU / g cottonseed meal; the specific fermentation process is as follows: crush the cottonseed meal and mix it with water in a mass ratio of 1:0.5, then add 1% molasses, mix thoroughly with a blender, and ferment in bags for 72 hours at a temperature controlled at 25-30°C; the fermentation is terminated when the pH drops below 4.5.

[0033] 2. Batching and Mixing: Weigh raw materials according to the formula ratio, with an error control of ±0.1%. Premixing: Trace ingredients (vitamins, minerals, drugs, etc.) are first mixed with a carrier (such as stone powder) to form a premix. This premix is ​​then mixed with the bulk raw materials (corn, soybean meal, etc.). Use a horizontal twin-shaft paddle mixer for 3-5 minutes, with a uniformity coefficient of variation (CV) ≤5%.

[0034] 3. Conditioning: Add steam (temperature 80-85°C, moisture 15-17%) to gelatinize the starch.

[0035] 4. Pelletizing: Press into pellets using a ring die pelletizer (pore size 2.0-2.5mm for 1-21d low soybean meal diets; 3.0-3.5mm for 21-100d low soybean meal diets). Cooling: Cool to room temperature (≤ ambient temperature + 5°C) using a countercurrent cooler, moisture ≤ 12%.

[0036] 5. Post-spraying: After granulation, enzyme preparations and oil are evenly added through the spray system.

[0037] 6. Packaging and storage.

[0038] Implementation 2:

[0039] The two-stage low soybean meal diet and the conventional diet of the present invention were respectively applied to Qingjiao Ma chickens, and their performance was evaluated:

[0040] 1 Materials and Methods

[0041] 1.1 Experimental design and diet

[0042] The study involved 360 one-day-old Qingjiao Ma chicken hens with an average body weight of 40.49 ± 1.10 g. A single-factor design was used with two treatments, six replicates per treatment, and 30 birds per replicate. No significant differences in initial broiler weights were observed between the groups (P > 0.05). The experiment lasted 100 days. Qingjiao Ma chickens were fed different diets in the early (1-21 days) and late (22-100 days) stages. The experimental design is shown in Table 1.

[0043] The control group diet used a corn-soybean meal basal diet, and the experimental group diet used a two-stage low-soybean meal diet for green-legged chickens provided by the present invention. Referring to the Chinese Chicken Feeding Standard (2004) and the Chinese Feed Composition and Nutritional Value Table (2010), the diets were formulated according to two stages: 1-21 days and 22-100 days. The experimental basal feed composition and nutritional levels are shown in Table 2.

[0044] Table 1 Diet composition and main nutrient levels of the control and experimental groups (air-dried basis)

[0045]

[0046] Each kg of premix provides: Vitamin A 8000 IU; Vitamin D3 1000 IU; Vitamin E 20 IU; Vitamin K 30.50 mg; Vitamin B1 2.00 mg; Vitamin B2 8.00 mg; Vitamin B6 3.50 mg; Vitamin B 12 10.00μg; niacin 35.00mg; calcium pantothenate 10.00mg; folic acid 0.55mg; biotin 0.18mg; Fe 80.00mg; Cu 8.00mg; Mn 100.00mg; Zn 80.00mg; I 0.70mg; Se 0.30mg.

[0047] 1.2 Feeding and management

[0048] The experimental broilers were raised on the ground with free access to food and water. One week before the chicks were introduced, the chicken coop and equipment were strictly cleaned and disinfected. A flue-type heating stove and fan were used to control the temperature. During the brooding period (0-21 days old), the temperature of the chicken coop should be controlled at 33-35°C and gradually reduced by 1-2°C every week. The humidity was maintained at 60%-65%, and the lighting time was gradually transitioned from 24 hours to 16 hours. The density was controlled at 25-30 per square meter to ensure the healthy development of the chicks. At the same time, a 1-21d first-stage feed diet should be provided. After entering the growth period (22-60 days old) and fattening period (61 days old-market), the temperature can be reduced to 22-26°C, the lighting is adjusted to natural light plus supplementary light, the density is reduced to 10 per square meter, and the diet is changed to a 21-100d second-stage feed diet. Follow the following immunization procedure:

[0049] Table 2 Immunization program of Qingjiaoma chicken

[0050]

[0051] 1.3 Experimental index determination

[0052] 1.3.1 Growth performance

[0053] Feed and water were withheld at 22:00 on the day before and after the experiment. The fasting weight of the broilers was measured at 08:00 the following day, and the body weight gain and average daily gain during the experimental period were calculated. At the end of the 100-day experiment, two birds were randomly selected from each replicate and slaughtered. The heart, liver, proventriculus, gizzard, breast muscle, leg muscle, and abdominal fat were weighed and the relative weights of each organ were calculated. The duodenum, jejunum, and ileum were isolated, and 1 cm segments of each were fixed with formaldehyde for histological examination.

[0054] 1.3.2 Analysis of serum biochemical indicators

[0055] The levels of serum total protein, albumin, glucose, triglycerides, total cholesterol, high-density lipoprotein, low-density lipoprotein, urea nitrogen, and uric acid, as well as the enzyme activities of alanine aminotransferase and aspartate aminotransferase were determined according to the manufacturer's instructions (Nanjing Jiancheng Bioengineering Institute, China).

[0056] 1.3.3 Intestinal paraffin sections

[0057] Select suitable intestinal tissue, put into fixative (as 10% neutral formalin) rapidly, the fixation time is generally 6-24 hours. It is cut into tissue blocks of appropriate size, usually 1-2cm long, 0.3-0.5cm thick. The fixed tissue is dehydrated through the ethanol of different concentrations (as 70%, 80%, 90%, 95%, 100%) successively, remove the moisture in the tissue, then carry out transparent treatment with dimethylbenzene etc., make tissue become transparent, be convenient to paraffin infiltration. The tissue after transparency is put into the paraffin that melts, in incubator, soak wax 2-3 hour, allow paraffin fully infiltrate into the tissue, then tissue is put into the paraffin that melts and embeds, make tissue wax block. Use microtome that wax block is cut into the thin slice that thickness is 3-5 micron, the thin slice cut is floated on the warm water surface, slice is flattened, then pick up and be attached on the slide. The sections are dewaxed in xylene, then hydrated with various concentrations of ethanol, and then stained, such as the commonly used hematoxylin-eosin (HE) stain, with hematoxylin staining the nuclei and eosin staining the cytoplasm. The staining time is determined based on the specific situation. The stained sections are then dehydrated with various concentrations of ethanol, transparentized with xylene, and finally mounted with neutral gum to form permanent sections for easy observation.

[0058] 1.4 Statistical analysis

[0059] The experimental data were analyzed by T-test program of SAS9.0 software. The results were expressed as mean values, and P<0.05 was considered significant.

[0060] 2 Results

[0061] 2.1 Effects of a two-stage low soybean meal diet on the growth performance of Qingjiao Ma chickens

[0062] As shown in Tables 3 and 4, compared with the control group diet, feeding the green-legged chickens with a two-stage low soybean meal diet had no significant effect on the survival rate, body weight, daily weight gain, feed intake and feed-to-meat ratio of 100-day-old green-legged chickens.

[0063] Table 3 Effects of two-stage low soybean meal diet on the survival rate of Qingjiao Ma chickens

[0064]

[0065] Table 4 Effects of two-stage low soybean meal diet on body weight, daily weight gain, feed intake and feed-to-meat ratio of Qingjiao Ma chickens

[0066]

[0067] 2.2 Effects of a two-stage low soybean meal diet on organ indices of Qingjiao Ma chickens

[0068] Table 5 shows that compared with the control diet, feeding the two-stage low-soybean meal diet to 100-day-old Qingjiao chickens significantly reduced abdominal fat and gizzard index (P<0.05), while significantly increased gizzard index (P<0.05). Furthermore, there were no significant differences in heart, liver, proventriculus, breast, or leg muscle indexes between the two groups (P>0.05).

[0069] Table 5 Effects of two-stage low soybean meal diet on organ indices of Qingjiao Ma chickens

[0070]

[0071] a and b indicate that there are significant differences between the data with different superscripts in the same column (p<0.05).

[0072] 2.3 Effects of a two-stage low soybean meal diet on blood biochemical parameters of Qingjiao Ma chickens

[0073] As shown in Table 6, when the green-legged chickens were fed a two-stage low-soybean meal diet, there were no significant changes in the total protein, albumin, total cholesterol, triglycerides, high-density lipoprotein, low-density lipoprotein and glucose levels in their blood, as well as the activities of alanine aminotransferase and aspartate aminotransferase (P>0.05), while the urea nitrogen and uric acid levels in their blood were significantly reduced (P<0.05).

[0074] Table 6 Effects of two-stage low soybean meal diet on blood biochemical parameters of Qingjiao Ma chickens

[0075]

[0076] a and b indicate that there are significant differences between the data with different superscripts in the same column (P<0.05).

[0077] 2.4 Effects of a two-stage low soybean meal diet on the intestinal morphology of Qingjiao Ma chickens

[0078] As shown in Table 7, feeding the two-stage low-soybean meal diet significantly increased jejunal villus height in 100-day-old Qingjiao chickens (P = 0.048), and there was a trend toward increasing ileal villus height, but the difference was not significant (P = 0.062). Otherwise, neither diet had a significant effect on duodenal villus height or crypt depth in the three intestinal segments of Qingjiao chickens (P > 0.05).

[0079] Table 7 Effects of two-stage low soybean meal diet on intestinal development of Qingjiao Ma chickens

[0080]

[0081] The means of a and b are significantly different in the columns without common superscripts (p<0.05).

[0082] 3 Conclusion

[0083] In summary, the two-stage low-soybean meal diet for Qingjiao Ma chickens provided by the present invention is completely suitable for raising Qingjiao Ma chickens and does not affect their production performance.

[0084] The above embodiments are intended only to illustrate the technical concepts and features of the present invention. Their purpose is to enable those skilled in the art to understand the contents of the present invention and implement them accordingly. They are not intended to limit the scope of protection of the present invention. Any equivalent changes or modifications made in accordance with the spirit of the present invention are intended to be covered by the scope of protection of the present invention.

Claims

1. A two-stage low soybean meal diet for green-legged chickens, characterized by: It includes low soybean meal diets for 1-21 days and low soybean meal diets for 21-100 days; The low soybean meal diet in the 1-21 day stage includes: soybean meal content of 8-10%; protein content of 18-20%; crystalline amino acid content of: lysine 0.9-1.2%, methionine 0.45-0.48%, threonine 0.68-0.72%, and tryptophan 0.24-0.32%; In the low soybean meal diet during the 21-100 d stage, the soybean meal content is 6-8%; the protein content is 16-19%; the crystalline amino acid content is: lysine 0.8-1.1%, methionine 0.36-0.40%, threonine 0.60-0.70%, and tryptophan 0.23-0.26%.

2. The two-stage low soybean meal diet for Qingjiao Ma chicken according to claim 1, characterized in that: The low soybean meal diet for the 1-21 d stage comprises, by weight, 540-560 parts of corn, 90-110 parts of corn starch residue, 90-100 parts of soybean meal, 40-60 parts of rice bran, 50-70 parts of corn gluten meal, 40-50 parts of corn distiller's grains DDGS, 45-55 parts of cottonseed meal, 7.0-8.0 parts of soybean oil, 8.0-9.0 parts of L-lysine, 1.4-1.8 parts of DL-methionine, 13-14 parts of rock powder, 8-9 parts of calcium hydrogen phosphate, 0.8-1.2 parts of choline chloride, 0.2-0.4 parts of enzyme preparation, 3.0-3.4 parts of sodium chloride, and 3.6-4.0 parts of premix.

3. The two-stage low soybean meal diet for Qingjiao Ma chicken according to claim 1, characterized in that: 500-510 parts of corn, 145-155 parts of corn starch residue, 60-80 parts of soybean meal, 30-35 parts of rice bran, 30-40 parts of corn gluten meal, 75-85 parts of corn distiller's grains DDGS, 45-55 parts of cottonseed meal, 35-45 parts of soybean oil, 8.0-8.5 parts of L-lysine, 0.8-1.0 part of DL-methionine, 13-14 parts of stone powder, 8.0-8.5 parts of calcium hydrogen phosphate, 0.6-1 part of choline chloride, 0.2-0.4 part of enzyme preparation, 3.0-3.5 parts of sodium chloride, and 3.0-4.0 parts of premix.

4. The two-stage low soybean meal diet for Qingjiao Ma chicken according to any one of claims 2 or 3, characterized in that: The enzyme preparation comprises acidic protease ≥50,000 U / g; xylanase ≥10,000 U / g; β-mannanase ≥3,000 U / g; cellulase ≥500 U / g; pectinase ≥1,000 U / g; phytase ≥500 U / g; and α-galactosidase ≥200 U / g.

5. The two-stage low soybean meal diet for Qingjiao Ma chicken according to any one of claims 2 or 3, characterized in that: The premix comprises 2.5-3.5 parts of mineral additive premix and 0.5-1.0 parts of vitamin additive premix in parts by weight.

6. The two-stage low soybean meal diet for Qingjiao Ma chicken according to claim 5, characterized in that: The mineral additive premix comprises 60.00-100.00 mg of Fe, 6.00-10.00 mg of Cu, 90.00-110.00 mg of Mn, 70.00-90.00 mg of Zn, 0.60-0.80 mg of I, and 0.20-0.40 mg of Se.

7. The two-stage low soybean meal diet for Qingjiao Ma chicken according to claim 5, characterized in that: The vitamin additive premix comprises VA 7000-9000 IU; VD3 800-1200 IU; VE 15-25 IU; VK3 0.40-0.60 mg; VB1 1.00-3.00 mg; VB2 6.00-10.00 mg; VB6 3.00-4.00 mg; VB 12 8.00-12.00 µg; niacin 30.00-40.00 mg; calcium pantothenate 8.00-12.00 mg; folic acid 0.50-0.60 mg; biotin 0.15-0.25 mg.

8. A method for preparing a two-stage low soybean meal diet for Qingjiao Ma chicken according to any one of claims 1 to 7, characterized in that: The specific steps are as follows: (1) Grind corn, soybean meal, and cottonseed meal to obtain crushed materials; the particle size of the crushed materials for the low soybean meal diet in the 1-21 day stage is 0.8-1.2 mm; the particle size of the crushed materials for the low soybean meal diet in the 21-100 day stage is 1.2-1.5 mm; (2) Weighing the raw materials according to the formula ratio, mixing the crushed material, the carrier and the premix to obtain a mixed material; (3) The mixed material is tempered, pelletized, cooled, and then sprayed with enzyme preparations and oil to obtain a low soybean meal diet.

9. The method for preparing a two-stage low-soybean meal diet for Qingjiao Ma chicken according to claim 8, characterized in that: In step (1), the cottonseed meal is fermented with a composite probiotic: the composite probiotic composition is: tropical Candida 1×10 8 CFU / g cottonseed meal, Lactobacillus plantarum 5×10 7 CFU / g cotton meal, Bacillus subtilis 5×10 7 CFU / g cottonseed meal, Aspergillus niger 1×10 6 CFU / g cottonseed meal; The specific fermentation process is: crush the cottonseed meal and mix it with water in a mass ratio of 1:0.5, then add 1% molasses, mix well, and ferment in bags for 72 hours. The temperature is controlled at 25~30℃; the fermentation is terminated when the pH drops below 4.

5.

10. Use of the two-stage low soybean meal diet for Qingjiao Ma chicken according to any one of claims 1 to 7 in Qingjiao Ma chicken breeding.

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