Preparation method and application of guarana extract

Abstract

The invention discloses a preparation method and application of a guarana extract, and relates to the field of guarana application, the preparation method comprises the following steps: mixing guarana powder with a culture medium as a guarana raw material, inoculating bifidobacterium, carrying out directional anaerobic fermentation under an anaerobic condition, adding cellulase after fermentation, and carrying out enzymatic conversion to obtain the guarana extract. The method comprises the following steps of: filtering a mixed solution, performing chromatography on the filtered mixed solution after filtering, performing chromatography on an extracting solution by utilizing a chromatography method, separating a useful extracting solution, concentrating the extracting solution by utilizing professional concentration equipment to reduce the moisture content in the extracting solution, drying the concentrated extracting solution, and leaving a dry extract, the preparation method comprises the following steps: crushing an extract, and sieving after crushing the extract to complete preparation of guarana extraction, so that the polyphenol content and water solubility are effectively improved, macromolecular impurities are removed, and the product is pure in flavor and simple and convenient to apply.

Description

Technical Field

[0001] The present invention relates to the application field of guarana, in particular to a preparation method and application of a guarana extract. Background Art

[0002] Guarana, also known as Brazilian cocoa, is an evergreen woody vine perennial plant native to the Amazon River basin. Guarana gum or guarana paste can be extracted from guarana seeds to prepare herbal stimulants. The short-term effects of guarana are mainly due to its high content of caffeine and related alkaloids, as well as a large amount of tannins. In addition, various saponins also play an important role in the pharmacology of drugs, especially the long-term consumption of guarana as a general tonic and preventive medicine, the effect is particularly significant. It is worth noting that the caffeine content in guarana seeds is extremely high, reaching 2.7% to 5.8% by dry weight. Therefore, guarana extracts are very popular in the global food industry and are used to produce soft drinks.

[0003] In existing guarana preparation methods, guarana fruit is cleaned and crushed, and then a specific ratio of dichloromethane, tetraethyl orthosilicate, and rhamnolipid are added as a soaking liquid. The extract is then filtered to obtain an extract. The extract is then added to the extract for extraction, and the composition and volume ratio of the extract affect the extraction effect. Finally, the resulting filtrate is dried to obtain a guarana extract.

[0004] When extracting the extract from guarana, the various liquid compositions mentioned above are used to soak the guarana to obtain an extract, which is then extracted using the extraction liquid. The composition of the soaking liquid and the extract will affect the quality of the extract itself and may cause impurities to be contained in the extract. Summary of the Invention

[0005] The object of the present invention is to solve the problem proposed in the above background art that the composition of the soaking liquid and the extracting liquid may affect the quality of the extract itself and may cause impurities in the extract. The present invention provides a preparation method of the guarana extract and its application.

[0006] In order to achieve the above-mentioned purpose, the present invention specifically adopts the following technical solutions:

[0007] A preparation method of guarana extract, the preparation method is as follows:

[0008] S1: First, inspect the guarana raw materials. After the raw materials are qualified, they can be used for production;

[0009] S2: washing and crushing the guarana raw material, mixing the guarana powder with a culture medium, inoculating bifidobacteria, and performing directed anaerobic fermentation under anaerobic conditions. After the fermentation is completed, cellulase is added for enzymatic conversion, and then the mixed solution is filtered using a membrane filter. After the filtration is completed, the filtered mixed solution is subjected to chromatography;

[0010] S3: After the extract and the extract solution are mixed, the extract solution and the mixed liquid are concentrated and sterilized;

[0011] S4: After concentrating and sterilizing the extract and the mixed solution, drying the mixed solution to convert the extract into a dry powder;

[0012] S5: Finally, the extract powder is crushed, and then the crushed extract is sieved to obtain a mixture with uniform composition, thereby improving the quality of the extract.

[0013] Furthermore, the acceptance of the guarana raw materials mainly tests items such as moisture, ash, pesticide residues, content, color, odor, and impurities, thereby improving the quality of the guarana and reducing the impact on the quality of the extract when extracting the guarana extract.

[0014] Furthermore, the extract is an ethanol solution of a certain concentration and volume, the membrane filtration is molecular-level filtration, and the chromatography uses macroporous resin chromatography. Thus, the extract can be extracted from guarana safely and energy-efficiently, while increasing the content of the extract, improving the solubility of the product, and further enriching the active ingredients in the extract.

[0015] Furthermore, the sterilization is a high-temperature sterilization method, and the drying is a spray drying method. The extract is concentrated using a concentration device to reduce the water content of the extract, and the high-temperature sterilization is used to kill the microorganisms and pathogens in the extract, thereby effectively inhibiting the growth of microorganisms in the extract and killing the microorganisms and pathogens in the extract.

[0016] Furthermore, the enzymatic conversion time in step S2 is 2-4 hours.

[0017] Furthermore, the directional anaerobic fermentation temperature is 30-37° C., the pH value is 5.5-6.5, and the fermentation time is 24-48 hours.

[0018] Furthermore, during the directional anaerobic fermentation process, natural antioxidants are added.

[0019] Furthermore, the natural antioxidant is vitamin C, which is used to prevent oxidation of the target component.

[0020] Furthermore, the pulverization is performed by physical machinery. The extract is pulverized by pulverizing equipment to obtain a powdered extract, thereby making the extract particles smaller and increasing the surface area, which is beneficial to subsequent work such as transportation, batching, and granulation.

[0021] The invention discloses an application of a guarana extract. The guarana extract can be applied to the food and beverage production industry, and the guarana extract can be used in the medical field.

[0022] In summary, the present invention includes at least one of the following beneficial effects:

[0023] 1. The present invention soaks the guarana raw material in an ethanol solution of a certain concentration and amount directly after the raw material is processed, and controls the temperature at the same time so that the effective ingredients in the guarana are leached as much as possible. Then, membrane filtration is used to achieve molecular-level filtration, so that the solvent, inorganic ions, small molecules, etc. pass through the membrane and retain particles and macromolecules. Then, the filtered extract is subjected to macroporous resin chromatography to remove impurities such as some polysaccharides, thereby achieving the purpose of being able to extract the extract from the guarana in an energy-saving and safe manner, reducing the possibility of affecting the quality of the extract, and simultaneously increasing the content of the extract, improving the solubility of the product, and further enriching the effective ingredients in the extract. Anaerobic microbial fermentation can destroy the cell structure, and the enzymatic reaction can accelerate the release of the target component. Through this extraction method, microorganisms and enzymes can be used for extraction, reducing the practicality of chemical reagents, and low-temperature fermentation and enzymatic reaction can retain the heat-sensitive active ingredients in the guarana to the greatest extent.

[0024] 2. The present invention uses a concentration device to concentrate the extract when drying the extract, thereby reducing the moisture in the extract, lowering the water activity, and inhibiting the growth of microorganisms. The concentrated extract is then sterilized at a high position to kill the microorganisms and pathogens in the extract. The extract is then sent to spray drying to effectively and quickly remove most of the moisture, thereby effectively inhibiting the growth of microorganisms in the extract, killing the microorganisms and pathogens in the extract, and maintaining the extract's good taste, color, and flavor. At the same time, the extract can be quickly dried to obtain an extract, which is beneficial to the transportation and preservation of the extract. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic flow diagram of the preparation method of the present invention. DETAILED DESCRIPTION

[0026] The following is combined with Figure 1 The present invention is described in further detail.

[0027] The embodiments of the present invention disclose a preparation method of a guarana extract and application thereof.

[0028] Reference Figure 1 , a preparation method of guarana extract, the preparation method is as follows:

[0029] S1: First, inspect the guarana raw materials. After the raw materials are qualified, they can be used for production;

[0030] S2: washing and crushing the guarana raw material, mixing the guarana powder with a culture medium, inoculating bifidobacteria, and performing directed anaerobic fermentation under anaerobic conditions. After the fermentation is completed, cellulase is added for enzymatic conversion, and then the mixed solution is filtered using a membrane filter. After the filtration is completed, the filtered mixed solution is subjected to chromatography;

[0031] S3: After the extract and the extract solution are mixed, the extract solution and the mixed liquid are concentrated and sterilized;

[0032] S4: After concentrating and sterilizing the extract and the mixed solution, drying the mixed solution to convert the extract into a dry powder;

[0033] S5: Finally, the extract powder is crushed, and then the crushed extract is sieved to obtain a mixture with uniform composition, thereby improving the quality of the extract.

[0034] When extracting guarana, the guarana raw materials are first collected, then tested, and sent to the extraction production line after passing the test. Then, the guarana raw materials are crushed into powder at a suitable temperature, and then the cell walls of the guarana raw materials are destroyed by the directional anaerobic fermentation of bifidobacteria therein. Specifically, the anaerobic microorganisms secrete organic acids during the fermentation process, which can lower the pH value of the environment and soften the cell wall structure. The extracellular enzymes produced by the microbial metabolism can directly decompose the cell wall polysaccharides, forming microporous channels and promoting the release of target components. The enzymatic conversion can be based on the fermentation. The residual structure of the cell wall is further decomposed to completely release the intracellular components. The extract is then concentrated using professional concentration equipment to reduce the water content in the extract. The concentrated extract is then dried to evaporate the water in the extract, leaving a dry extract. The extract is then crushed. After the extract is crushed, it is sieved to complete the preparation of the guarana extract. When preparing the guarana extract, the extract is treated with membrane filtration and chromatography, thereby effectively increasing the polyphenol content and water solubility, while removing large molecular impurities, making the product pure in flavor and easy to use.

[0035] Guarana raw material acceptance inspections primarily focus on testing for moisture, ash, pesticide residue, content, color, odor, and impurities. Guarana can only be used in production after passing these inspections. During the acceptance inspection of guarana raw materials, various indicators and surface residues are tested to improve the quality of the guarana and minimize any impact on the quality of the guarana extract.

[0036] After directional anaerobic fermentation and enzymatic conversion, membrane filtration is used to achieve molecular-level filtration, allowing solvents, inorganic ions, small molecules, etc. to pass through the membrane, while retaining particles and macromolecules. The filtered extract is then subjected to macroporous resin chromatography to remove some impurities such as polysaccharides, thereby enabling the extract to be leached from guarana in an energy-saving and safe manner, while increasing the extract content, improving the solubility of the product, and enriching the active ingredients in the extract.

[0037] Sterilization is performed using a high-temperature sterilization method, and drying is performed using a spray drying method. When drying the extract, a concentration device is used to concentrate the extract, reducing the water content in the extract, lowering water activity, and inhibiting microbial growth. The concentrated extract is then sterilized at a high position to kill the microorganisms and pathogens in the extract. The extract is then spray-dried to effectively and quickly remove most of the water, thereby effectively inhibiting the growth of microorganisms in the extract, killing the microorganisms and pathogens in the extract, and maintaining a good taste, color, and flavor. The extract can also be quickly dried to obtain an extract, which is beneficial for the transportation and storage of the extract.

[0038] Pulverization is done using physical machinery. After drying the extract to obtain the extract, the extract is pulverized using pulverizing equipment to obtain a powdered extract. This can reduce the extract particles and increase the surface area, facilitating subsequent work such as transportation, batching, and granulation.

[0039] An application of guarana extract. The guarana extract can be used in the production of food and beverage industries. The guarana extract can be used in the medical field to treat symptoms such as fatigue, depression, diarrhea, pain, and fever. The tannin and saponin components in guarana have anti-inflammatory, antioxidant and antibacterial effects.

Claims

1. A method for preparing a guarana extract, characterized in that: The preparation method is as follows: S1: First, inspect the guarana raw materials. Once the raw materials are qualified, they can be used for production; S2: washing and crushing the guarana raw material, mixing the guarana powder with a culture medium, inoculating bifidobacteria, and performing directed anaerobic fermentation under anaerobic conditions. After the fermentation is completed, cellulase is added for enzymatic conversion, and then the mixed solution is filtered using a membrane filter. After the filtration is completed, the filtered mixed solution is subjected to chromatography; S3: After the extract and the extract solution are mixed, the extract solution and the mixed liquid are concentrated and sterilized; S4: After concentrating and sterilizing the extract and the mixed solution, drying the mixed solution to convert the extract into a dry powder; S5: Finally, the extract powder is crushed, and then the crushed extract is sieved to obtain a mixture with uniform composition, thereby improving the quality of the extract.

2. The method for preparing a guarana extract according to claim 1, wherein: The acceptance inspection of the guarana raw materials mainly tests items such as moisture, ash, pesticide residues, content, color, odor, impurities, etc. Only after passing the acceptance inspection can they be put into production and use.

3. The method for preparing a guarana extract according to claim 1, wherein: The extract is an ethanol solution of a certain concentration and multiple volume, the membrane filtration is molecular-level filtration, and the chromatography adopts macroporous resin chromatography.

4. The method for preparing a guarana extract according to claim 1, wherein: The sterilization is a high-temperature sterilization method, and the drying is a spray drying method.

5. The method for preparing a guarana extract according to claim 1, wherein: The pulverization is performed by physical machinery.

6. The method for preparing a guarana extract according to claim 1, wherein: The enzymatic conversion time in step S2 is 2-4 hours.

7. The method for preparing a guarana extract according to claim 1, wherein: The temperature of the directional anaerobic fermentation is 30-37° C., the pH value is 5.5-6.5, and the fermentation time is 24-48 hours.

8. The method for preparing a guarana extract according to claim 1, wherein: During the directional anaerobic fermentation process, natural antioxidants are added.

9. The method for preparing a guarana extract according to claim 8, wherein: The natural antioxidant is vitamin C, which is used to prevent the target component from being oxidized.

10. A use of a guarana extract, characterized in that: The guarana extract can be applied to the food and beverage production industry. The guarana extract can be used in the medical field for treating symptoms such as fatigue, depression, diarrhea, pain, and fever. The tannin and saponin components in the guarana have anti-inflammatory, antioxidant and antibacterial effects.

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