Microbial treatment flora for treating odor of household garbage treatment station

Abstract

The invention discloses a microbiological treatment flora for treating odor of a household garbage treatment station. Comprising the following microbial treatment flora components: 12 to 33 percent of biphasic photosynthetic bacteria, 0.2 to 1.5 percent of clostridium butyricum, 8 to 22 percent of bifidobacterium, 0.2 to 1.5 percent of nitrogen-fixing bacteria, 9 to 18 percent of mesophilic actinomycetes, 0.3 to 2 percent of potassium bacteria, 13 to 23 percent of subdigestion bacteria, 0.3 to 2 percent of phosphate solubilizing bacteria, 18 to 24 percent of bacillus megatherium, 0.5 to 3 percent of lactic acid bacteria and 0.5 to 3 percent of saccharomycetes. Fatty acids and sulfides are decomposed through the biphase photosynthetic bacteria through photosynthesis, stink precursor substances are reduced from the source, bifidobacteria and lactic acid bacteria reduce the pH of the environment through acid production, growth of stink-producing putrefying bacteria such as escherichia coli and salmonella is inhibited, release of stink gases such as ammonia and methyl mercaptan is reduced, saccharomycetes generate alcohol through saccharides, and the alcohol content is reduced. The removal rate of hydrogen sulfide and ammonia is greater than or equal to 80%, and the degradation rate of the volatile fatty acid is greater than or equal to 70%.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial treatment bacterial communities, in particular to a microbial treatment bacterial community for treating odor in a domestic waste treatment station. Background Art

[0002] As people's living standards improve, more and more garbage is generated in their lives and work. After being discarded, the garbage is transported to garbage stations for treatment. Garbage stations will produce a lot of odor when working, and some of the odor will directly affect people's health. At present, many garbage stations are only flushed to clean them during cleaning. Not only does it fail to remove the odor, but it also causes some garbage leachate to flow away, causing damage to the surrounding environment. For this reason, we provide a microbial treatment flora for treating the odor of domestic garbage treatment stations. Summary of the Invention

[0003] The purpose of the present invention is to provide a microbial treatment flora for treating odor in domestic waste treatment stations, so as to solve the problems raised in the above-mentioned background technology.

[0004] To achieve the above-mentioned objectives, the present invention provides the following technical solution: a microbial treatment flora for treating odors in domestic waste treatment stations, comprising the following microbial treatment flora components, calculated by weight percentage: 12-33% of biphasic photosynthetic bacteria, 0.2-1.5% of Clostridium butyricum, 8-22% of Bifidobacterium, 0.2-1.5% of nitrogen-fixing bacteria, 9-18% of mesophilic actinomycetes, 0.3-2% of potassium-solubilizing bacteria, 13-23% of subdigestible bacteria, 0.3-2% of phosphate-solubilizing bacteria, 18-24% of Bacillus megaterium, 0.5-3% of lactic acid bacteria, and 0.5-3% of yeast.

[0005] Preferably, biphasic photosynthetic bacteria 12%, Clostridium butyricum 0.2%, Bifidobacterium 8%, nitrogen-fixing bacteria 0.2%, mesophilic actinomycetes 9%, potassium-solubilizing bacteria 0.3%, subdigestible bacteria 13%, phosphate-solubilizing bacteria 0.3%, Bacillus megaterium 18%, lactic acid bacteria 0.5%, and yeast 0.5%.

[0006] Preferably, biphasic photosynthetic bacteria 24%, Clostridium butyricum 1%, Bifidobacterium 16%, nitrogen-fixing bacteria 1%, mesophilic actinomycetes 14%, potassium-solubilizing bacteria 1%, subdigestible bacteria 18%, phosphate-solubilizing bacteria 1.5%, Bacillus megaterium 20%, lactic acid bacteria 2%, and yeast 1.5%.

[0007] Preferably, biphasic photosynthetic bacteria 33%, Clostridium butyricum 1.5%, Bifidobacterium 22%, nitrogen-fixing bacteria 1.5%, mesophilic actinomycetes 18%, potassium-solubilizing bacteria 2%, subdigestible bacteria 23%, phosphate-solubilizing bacteria 2%, Bacillus megaterium 24%, lactic acid bacteria 3%, and yeast 3%.

[0008] Preferably, the biphasic photosynthetic bacteria is a mixture of Rhodopseudomonas and green sulfur bacteria, with a weight ratio of 1:2 to 2:1.

[0009] Preferably, the number of viable bacteria of Bacillus megaterium is ≥8.0×107 CFU / g, the number of viable bacteria of Bifidobacterium is ≥3.0×107 CFU / g, the number of viable bacteria of diphasic photosynthetic bacteria is ≥1.0×108 CFU / g, the number of viable bacteria of mesophilic actinomycetes is ≥5.0×107 CFU / g, the number of viable bacteria of subdigestible bacteria is ≥6.0×107 CFU / g, the number of viable bacteria of lactic acid bacteria is ≥4.0×107 CFU / g, the number of viable bacteria of yeast is ≥3.0×107 CFU / g, the number of viable bacteria of phosphate-solubilizing bacteria is ≥2.0×107 CFU / g, the number of viable bacteria of potassium-solubilizing bacteria is ≥2.0×107 CFU / g, the number of viable bacteria of nitrogen-fixing bacteria is ≥1.5×107 CFU / g, and the number of viable bacteria of Clostridium butyricum is ≥1.5×107 CFU / g.

[0010] Preferably, the lactic acid bacteria is Lactobacillus plantarum or Lactobacillus acidophilus, the yeast is Saccharomyces cerevisiae or Candida utilis, the phosphate-solubilizing bacteria is Pseudomonas or Bacillus, and the potassium-solubilizing bacteria is Bacillus gelatinosa or silicate bacteria.

[0011] Compared with the prior art, the present invention has the following beneficial effects:

[0012] 1. The present invention uses biphasic photosynthetic bacteria to decompose fatty acids and sulfides through photosynthesis, reducing odor precursors from the source. Bifidobacteria and lactic acid bacteria lower the environmental pH by producing acid, inhibiting the growth of odor-producing spoilage bacteria such as Escherichia coli and Salmonella, and reducing the release of odorous gases such as ammonia and methyl mercaptan. Yeast uses sugars to produce alcohol, blocking the synthesis pathway of volatile fatty acids such as butyric acid and valeric acid. The removal rate of hydrogen sulfide and ammonia is ≥80%, and the degradation rate of volatile fatty acids is ≥70%. Mesophilic actinomycetes secrete extracellular enzymes to decompose macromolecular organic matter such as protein and cellulose in garbage. Subdigestible bacteria accelerate the anaerobic digestion process and reduce the accumulation of amine substances. Phosphate-solubilizing bacteria, potassium-solubilizing bacteria and nitrogen-fixing bacteria promote the circulation of nitrogen, phosphorus and potassium, reduce the odor caused by nitrogen loss, and at the same time improve the maturity of garbage, fundamentally inhibiting odor regeneration.

[0013] 2. The present invention does not contain any chemical agents, can be naturally degraded, has no secondary pollution, and has strong adaptability, is suitable for different seasons, and can withstand high organic matter loads. At the same time, it is low in cost, the strains are easy to culture, and the dosage is small. The treatment cost is reduced by more than 50% compared with traditional methods. In addition, while the bacterial community degrades organic matter, it can convert nitrogen, phosphorus and potassium in the garbage into usable forms. When used with carriers such as rice husks and sawdust, the treated garbage residue can be used as a raw material for organic fertilizer, achieving the dual goals of deodorization and resource utilization, which is in line with the concept of circular economy. DETAILED DESCRIPTION

[0014] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0015] Example 1

[0016] A microbial treatment flora for treating odors in a domestic waste treatment station comprises the following microbial treatment flora components, measured in percentage by weight: 12% of biphasic photosynthetic bacteria, 0.2% of Clostridium butyricum, 8% of Bifidobacterium, 0.2% of nitrogen-fixing bacteria, 9% of mesophilic actinomycetes, 0.3% of potassium-solubilizing bacteria, 13% of subdigestible bacteria, 0.3% of phosphate-solubilizing bacteria, 18% of Bacillus megaterium, 0.5% of lactic acid bacteria, and 0.5% of yeast. The biphasic photosynthetic bacteria are a mixture of Rhodopseudomonas and green sulfur bacteria, and the weight ratio of the two is 1:2-2:1.

[0017] The number of viable bacteria of Bacillus megaterium was 8.0×107 CFU / g, the number of viable bacteria of Bifidobacterium was 3.0×107 CFU / g, the number of viable bacteria of biphasic photosynthetic bacteria was 1.0×108 CFU / g, the number of viable bacteria of mesophilic actinomycetes was 5.0×107 CFU / g, the number of viable bacteria of subdigestible bacteria was 6.0×107 CFU / g, the number of viable bacteria of lactic acid bacteria was 4.0×107 CFU / g, the number of viable bacteria of yeast was 3.0×107 CFU / g, the number of viable bacteria of phosphate-solubilizing bacteria was The viable bacteria count is 2.0×107 CFU / g, the viable bacteria count of potassium-solubilizing bacteria is 2.0×107 CFU / g, the viable bacteria count of nitrogen-fixing bacteria is 1.5×107 CFU / g, and the viable bacteria count of Clostridium butyricum is 1.5×107 CFU / g. The lactic acid bacteria are Lactobacillus plantarum or Lactobacillus acidophilus, the yeast is Saccharomyces cerevisiae or Candida utilis, the phosphate-solubilizing bacteria are Pseudomonas or Bacillus phosphate-solubilizing bacteria, and the potassium-solubilizing bacteria are jelly-like Bacillus or silicate bacteria.

[0018] Example 2

[0019] A microbial treatment flora for treating odors in domestic waste treatment stations comprises the following microbial treatment flora components, measured by weight percentage: 24% of biphasic photosynthetic bacteria, 1% of Clostridium butyricum, 16% of Bifidobacterium, 1% of nitrogen-fixing bacteria, 14% of mesophilic actinomycetes, 1% of potassium-solubilizing bacteria, 18% of subdigestible bacteria, 1.5% of phosphate-solubilizing bacteria, 20% of Bacillus megaterium, 2% of lactic acid bacteria, and 1.5% of yeast. The biphasic photosynthetic bacteria are a mixture of Rhodopseudomonas and green sulfur bacteria, and the weight ratio of the two is 1:2-2:1.

[0020] The number of viable bacteria of Bacillus megaterium was 2.4×108 CFU / g, the number of viable bacteria of Bifidobacterium was 8.0×107 CFU / g, the number of viable bacteria of biphasic photosynthetic bacteria was 1.8×108 CFU / g, the number of viable bacteria of mesophilic actinomycetes was 7.0×107 CFU / g, the number of viable bacteria of subdigestible bacteria was 9.0×107 CFU / g, the number of viable bacteria of lactic acid bacteria was 6.0×107 CFU / g, the number of viable bacteria of yeast was 3.3×107 CFU / g, and the number of viable bacteria of phosphate-solubilizing bacteria was 1. The viable bacteria count of the lactic acid bacteria is 2.2×107 CFU / g, the viable bacteria count of the potassium-solubilizing bacteria is 2.5×107 CFU / g, the viable bacteria count of the nitrogen-fixing bacteria is 2×107 CFU / g, and the viable bacteria count of the butyric acid Clostridium is 2×107 CFU / g. The lactic acid bacteria are Lactobacillus plantarum or Lactobacillus acidophilus, the yeast is Saccharomyces cerevisiae or Candida utilis, the phosphate-solubilizing bacteria are Pseudomonas or Bacillus phosphate-solubilizing bacteria, and the potassium-solubilizing bacteria are jelly-like Bacillus or silicate bacteria.

[0021] Example 3

[0022] A microbial treatment flora for treating odors in a domestic waste treatment station comprises the following microbial treatment flora components, measured by weight percentage: 33% of biphasic photosynthetic bacteria, 1.5% of Clostridium butyricum, 22% of Bifidobacterium, 1.5% of nitrogen-fixing bacteria, 18% of mesophilic actinomycetes, 2% of potassium-solubilizing bacteria, 23% of subdigestible bacteria, 2% of phosphate-solubilizing bacteria, 24% of Bacillus megaterium, 3% of lactic acid bacteria, and 3% of yeast. The biphasic photosynthetic bacteria are a mixture of Rhodopseudomonas and green sulfur bacteria, and the weight ratio of the two is 1:2-2:1.

[0023] The number of viable bacteria of Bacillus megaterium was 2.4×108CFU / g, the number of viable bacteria of Bifidobacterium was 1.1×108CFU / g, the number of viable bacteria of biphasic photosynthetic bacteria was 3.3×108CFU / g, the number of viable bacteria of mesophilic actinomycetes was 9.0×107CFU / g, the number of viable bacteria of subdigestible bacteria was 1.2×108CFU / g, the number of viable bacteria of lactic acid bacteria was 6.0×107CFU / g, the number of viable bacteria of yeast was 4.5×107CFU / g, the number of viable bacteria of phosphate-solubilizing bacteria was The viable bacteria count is 8.0×107 CFU / g, the viable bacteria count of potassium-solubilizing bacteria is 8.0×107 CFU / g, the viable bacteria count of nitrogen-fixing bacteria is 7.0×107 CFU / g, and the viable bacteria count of Clostridium butyricum is 5.0×107 CFU / g. The lactic acid bacteria are Lactobacillus plantarum or Lactobacillus acidophilus, the yeast is Saccharomyces cerevisiae or Candida utilis, the phosphate-solubilizing bacteria are Pseudomonas or Bacillus phosphate-solubilizing bacteria, and the potassium-solubilizing bacteria are jelly-like Bacillus or silicate bacteria.

[0024] It can be seen from the above three embodiments that embodiment 2 is the optimal solution. Biphasic photosynthetic bacteria decompose fatty acids and sulfides through photosynthesis, reducing odor precursors from the source. Bifidobacteria and lactic acid bacteria reduce the pH of the environment by producing acid, inhibit the growth of odor-producing spoilage bacteria such as Escherichia coli and Salmonella, and reduce the release of odorous gases such as ammonia and methyl mercaptan. Yeast uses sugars to produce alcohol, blocking the synthesis path of volatile fatty acids such as butyric acid and valeric acid. The removal rate of hydrogen sulfide and ammonia is ≥80%, and the degradation rate of volatile fatty acids is ≥70%. Mesophilic actinomycetes secrete extracellular enzymes to decompose macromolecular organic matter such as protein and cellulose in garbage. Subdigestible bacteria accelerate the anaerobic digestion process and reduce Amine accumulation; phosphate-solubilizing bacteria, potassium-solubilizing bacteria and nitrogen-fixing bacteria promote the cycle of nitrogen, phosphorus and potassium, reduce the stench caused by nitrogen loss, and improve the maturity of garbage, fundamentally inhibiting odor regeneration, without chemical agents, naturally degradable, no secondary pollution, and strong adaptability, suitable for different seasons, and resistant to high organic matter loads. At the same time, the cost is low, the strains are easy to cultivate, the dosage is small, and the treatment cost is reduced by more than 50% compared with traditional methods. Moreover, while the bacterial community degrades organic matter, it can convert nitrogen, phosphorus and potassium in the garbage into usable forms. When used with carriers such as rice husks and sawdust, the treated garbage residues can be used as raw materials for organic fertilizers, achieving the dual goals of deodorization and resource utilization, which is in line with the concept of circular economy.

[0025] A method for preparing a microbial flora for treating odor in a domestic waste treatment station, comprising the following preparation methods:

[0026] S1. Diphasic photosynthetic bacteria: Rhodopseudomonas and green sulfur bacteria were inoculated separately into a light culture medium containing 3% glucose, 0.3% magnesium sulfate, and 0.1% yeast extract. The culture was carried out at a light intensity of 2500 lux and a temperature of 30±2°C for 48-72 hours to a viable bacterial concentration of ≥1.0×108 CFU / g.

[0027] S2. Bifidobacterium: Inoculate in anaerobic culture medium containing 1% glucose, 0.5% beef extract, and 0.05% cysteine ​​hydrochloride, and culture at 37±1°C for 48-60 hours to a viable bacterial concentration of ≥3.0×107 CFU / g.

[0028] S3. Mesophilic actinomycetes: Inoculate into Gao's medium No. 1 and culture at 32±2℃ for 5-7 days until the viable bacteria concentration is ≥5.0×107 CFU / g.

[0029] S4. Subdigestible bacteria: Inoculate in an inorganic salt medium containing 0.5% peptone and 0.2% ammonium chloride, and culture with shaking at 35±2°C and 180 rpm for 36-48 hours to a viable bacterial concentration of ≥6.0×107 CFU / g.

[0030] S5. Bacillus megaterium: Inoculate into LB medium and culture with shaking at 37±1°C and 200 rpm for 24-36 hours to a viable bacterial concentration of ≥8.0×107 CFU / g.

[0031] S6. Lactic acid bacteria: Taking Lactobacillus plantarum as an example, inoculate it into MRS medium and culture it under anaerobic conditions at 37±1℃ for 24-36 hours to a viable bacteria concentration of ≥4.0×107 CFU / g.

[0032] S7. Yeast: Using Saccharomyces cerevisiae as an example, inoculate into YPD medium and culture with shaking at 28±2°C and 150 rpm for 18-24 hours to a viable bacterial concentration ≥3.0×107 CFU / g.

[0033] S8. Phosphate-solubilizing bacteria: Taking Pseudomonas phosphate-solubilizing bacteria as an example, inoculate them into Montgena inorganic phosphate medium and culture them at 30±2℃ and 160rpm with shaking for 48-72 hours to a viable bacteria concentration of ≥2.0×107 CFU / g.

[0034] S9. Potassium-solubilizing bacteria: Taking Bacillus gelatinus as an example, inoculate it into silicate bacterial culture medium and culture it at 30±2℃ and 180 rpm with shaking for 48-72 hours to a viable bacteria concentration ≥2.0×107 CFU / g.

[0035] S10. Nitrogen-fixing bacteria: Taking nitrogen-fixing bacteria of the genus Rhizobium as an example, inoculate into nitrogen-free culture medium and culture with shaking at 28±2°C and 120 rpm for 72-96 hours to a viable bacteria concentration ≥1.5×107 CFU / g.

[0036] S11. Clostridium butyricum: Inoculate into a reinforced Clostridium medium and culture under anaerobic conditions at 37±1°C for 48-72 hours to a viable bacteria concentration ≥1.5×107 CFU / g.

[0037] S12. Bacterial liquid treatment: centrifuge the culture liquid of each bacterial species (4000 rpm, 10 minutes), discard the supernatant and resuspend in sterile saline to prepare a bacterial suspension with a viable bacterial concentration of ≥5.0×108 CFU / g.

[0038] S13. Mixing and compounding: 24% of biphasic photosynthetic bacteria, 1% of Clostridium butyricum, 16% of Bifidobacterium, 1% of nitrogen-fixing bacteria, 14% of mesophilic actinomycetes, 1% of potassium-solubilizing bacteria, 18% of subdigestible bacteria, 1.5% of phosphate-solubilizing bacteria, 20% of Bacillus megaterium, 2% of lactic acid bacteria, and 1.5% of yeast are mixed with each bacterial suspension, 0.2% of sodium alginate by weight of the mixed solution is added as a protective agent, and a sterile buffer solution (pH 6.5-7.5) is added at the same time to adjust the total viable bacteria concentration to (5.0-8.0)×108 CFU / g, and the microbial treatment bacterial community is obtained after stirring evenly.

[0039] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A microbial treatment bacterial community for treating odor in domestic waste treatment stations, characterized in that: Calculated by weight percentage, the invention includes the following microbial treatment bacterial community components: 12-33% of biphasic photosynthetic bacteria, 0.2-1.5% of Clostridium butyricum, 8-22% of Bifidobacterium, 0.2-1.5% of nitrogen-fixing bacteria, 9-18% of mesophilic actinomycetes, 0.3-2% of potassium-solubilizing bacteria, 13-23% of subdigestible bacteria, 0.3-2% of phosphate-solubilizing bacteria, 18-24% of Bacillus megaterium, 0.5-3% of lactic acid bacteria, and 0.5-3% of yeast.

2. The microbial treatment bacterial community for treating odor in a domestic waste treatment station according to claim 1, characterized in that: Biphasic photosynthetic bacteria 12%, Clostridium butyricum 0.2%, Bifidobacterium 8%, nitrogen-fixing bacteria 0.2%, mesophilic actinomycetes 9%, potassium-solubilizing bacteria 0.3%, subdigestible bacteria 13%, phosphate-solubilizing bacteria 0.3%, Bacillus megaterium 18%, lactic acid bacteria 0.5%, and yeast 0.5%.

3. The microbial treatment bacterial community for treating odor in a domestic waste treatment station according to claim 2, characterized in that: Biphasic photosynthetic bacteria 24%, Clostridium butyricum 1%, Bifidobacterium 16%, nitrogen-fixing bacteria 1%, mesophilic actinomycetes 14%, potassium-solubilizing bacteria 1%, subdigestible bacteria 18%, phosphate-solubilizing bacteria 1.5%, Bacillus megaterium 20%, lactic acid bacteria 2%, and yeast 1.5%.

4. The microbial treatment bacterial community for treating odor in a domestic waste treatment station according to claim 3, characterized in that: Biphasic photosynthetic bacteria 33%, Clostridium butyricum 1.5%, Bifidobacterium 22%, nitrogen-fixing bacteria 1.5%, mesophilic actinomycetes 18%, potassium-solubilizing bacteria 2%, subdigestible bacteria 23%, phosphate-solubilizing bacteria 2%, Bacillus megaterium 24%, lactic acid bacteria 3%, and yeast 3%.

5. The microbial treatment bacterial community for treating malodor in a domestic waste treatment station according to claim 4, characterized in that: The diphasic photosynthetic bacteria are a mixture of Rhodopseudomonas and green sulfur bacteria, with a weight ratio of 1:2-2:

1.

6. The microbial treatment bacterial community for treating odor in a domestic waste treatment station according to claim 5, characterized in that: The number of viable bacteria of Bacillus megaterium is ≥8.0×107CFU / g, the number of viable bacteria of Bifidobacterium is ≥3.0×107CFU / g, the number of viable bacteria of diphasic photosynthetic bacteria is ≥1.0×108CFU / g, the number of viable bacteria of mesophilic actinomycetes is ≥5.0×107CFU / g, the number of viable bacteria of subdigestible bacteria is ≥6.0×107CFU / g, the number of viable bacteria of lactic acid bacteria is ≥4.0×107CFU / g, the number of viable bacteria of yeast is ≥3.0×107CFU / g, the number of viable bacteria of phosphate-solubilizing bacteria is ≥2.0×107CFU / g, the number of viable bacteria of potassium-solubilizing bacteria is ≥2.0×107CFU / g, the number of viable bacteria of nitrogen-fixing bacteria is ≥1.5×107CFU / g, and the number of viable bacteria of Clostridium butyricum is ≥1.5×107CFU / g.

7. The microbial treatment bacterial community for treating malodor in a domestic waste treatment station according to claim 6, characterized in that: The lactic acid bacteria are Lactobacillus plantarum or Lactobacillus acidophilus, the yeast is Saccharomyces cerevisiae or Candida utilis, the phosphate-solubilizing bacteria are Pseudomonas or Bacillus phosphate-solubilizing bacteria, and the potassium-solubilizing bacteria are Bacillus gelatinus or silicate bacteria.