Method for artificially planting sea grapes

Abstract

The invention belongs to the technical field of aquaculture, and particularly relates to a method for artificially planting sea grapes. The method comprises the steps that sea grape seedlings are temporarily cultured in a temporary culture pond and then put into a culture pond to be cultured after being temporarily cultured, red light and blue light are alternately irradiated in the culture period, and fertilization is conducted twice. Sea grape seedlings are temporarily cultured in the culture pond and then put into the culture pond to be planted, stress resistance and disease resistance of the sea grapes are improved, dependence on the environment is reduced, the survival rate of the seedlings is increased, red and blue light alternate irradiation is beneficial to improvement of photosynthetic efficiency of the sea grapes, and then the content of nutrient substances in the sea grapes is increased; and the quality is optimized.

Description

Technical Field

[0001] The invention belongs to the technical field of aquaculture, and particularly relates to a method for artificially planting sea grapes. Background Art

[0002] Sea grapes are an edible seaweed belonging to the Chlorophyta ( chlorophyta ), Pteridaceae ( caulerpaceae )Pteridium( Caulerpa Sea grapes are named "sea grapes" for their round, plump, and attractively colored bulbous branches on their upright stems, resembling crystal-clear clusters of grapes. They are also known as "green caviar" for their distinctive caviar flavor and texture. They are rich in essential polysaccharides, carotene, vitamins, and various minerals. They are also rich in protein, polyphenols, polysaccharides, dietary fiber, omega-3 and omega-6 unsaturated fatty acids, linolenic acid, arachidonic acid (ARA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA). They contain 11 of the 17 essential amino acids, including aspartic acid and proline, which are the most abundant and contribute to their distinctive aroma and flavor. Sea grapes are a low-calorie, cholesterol-free, green and healthy vegetable. They are effective in treating high blood sugar, high blood lipids, constipation, neutralizing stomach acid, and treating pathogens such as Helicobacter pylori. Long-term consumption can enhance immunity, improve beauty, slow aging, promote intellectual development, and strengthen physical fitness.

[0003] Based on this, developing a sea grape cultivation technique to increase its nutrient content not only has broad market prospects but also holds significant significance for promoting the development of the health food industry. This would not only enhance the nutritional value of sea grapes, satisfying consumer demand for high-quality, healthy foods, but also provide new impetus for sustainable agricultural development and food innovation. Summary of the Invention

[0004] The purpose of the present invention is to provide a method for artificially cultivating sea grapes, thereby increasing the content of nutrients in sea grapes, improving their quality and ensuring stable yield.

[0005] The present invention provides a method for artificially growing sea grapes, the method comprising the following steps: Seedling temporary rearing: After the temporary rearing pond is disinfected, it is filled with filtered seawater at a height of 0.5-1m. Healthy sea grapes with upright stems of 10-15cm are selected, cleaned, and impurities removed. After sowing, they are planted on the substrate and placed in the temporary rearing pond for 5-7 days. The sowing amount is 500-800g / m 2 The temporary culture temperature is 23 ℃ ~ 30 ℃, the light source is natural light, the light intensity is 5000 ~ 8000 Lux, and the light-dark ratio is 12h / 12h; Stocking of seedlings: After the culture pond is disinfected, it is filled with filtered seawater. The seedlings are taken out from the temporary holding pond and placed in the culture pond for cultivation. The seeding rate is 1500~2000g / m 2 ; When the culture is to the 10th day, the first fertilization is carried out, and when the culture is to the 30th day, the second fertilization is carried out; the culture temperature is 25 ℃ ~ 28 ℃, the light source is red and blue light, the red and blue light irradiation mode is 30 minutes red light / 30 minutes blue light alternating irradiation, the light intensity is 5000 ~ 8000 Lux, and the light-dark ratio is 16h / 8h; Purification culture: After disinfection, the purification pool is injected with artificial seawater with a salinity of 2.5% to 2.8%. On the 90th day of cultivation, the sea grapes are transferred to the purification pool for purification culture. The purification culture temperature is 25°C to 28°C, the light source is natural light, the light intensity is 5000-8000 Lux, and the light-dark ratio is 12h / 12h. Harvest: Harvest after 20 to 30 days of purification culture.

[0006] Preferably, the disinfection method is sunlight exposure and / or potassium permanganate disinfection.

[0007] Preferably, the filtration pore size is 60-80 mesh.

[0008] Preferably, the variety of sea grapes is long stem grape fern algae ( Caulerpa lentillifera ).

[0009] Preferably, the attachment base is a polyethylene mesh cage.

[0010] Preferably, the temperature of the temporary cultivation is 23°C to 30°C, and the light intensity is 3000 to 8000 Lux.

[0011] Preferably, the height of the seawater in the culture pond is 1.5-2 m.

[0012] Preferably, during the cultivation period, the algae are manually removed every 10 days.

[0013] Preferably, the fertilization method is to sprinkle fertilizer into the breeding pond.

[0014] Preferably, the amount of fertilizer used in the first fertilization is: 15-30 g / m 2 , slaked lime 5~10g / m 2 , sodium hydrogen phosphate 4~9g / m 2 , dipotassium hydrogen phosphate 2~6g / m 2 , humic acid iron 3~8g / m 2 and ammonium ferric citrate 3~5g / m 2 .

[0015] Preferably, the fertilizer dosage for the second fertilization is: 20-35 g / m 2 , slaked lime 5~10g / m 2 , sodium hydrogen phosphate 8~12g / m 2 , dipotassium hydrogen phosphate 5~8g / m 2 , humic acid iron 6~10g / m 2 and ammonium ferric citrate 5~8g / m 2 .

[0016] Beneficial effects of the present invention: The present invention temporarily raises sea grape seedlings in a culture pond, which helps to improve the stress resistance of sea grapes, enhance their disease resistance, reduce their dependence on the environment, and increase the survival rate of seedlings. Even if no artificial algae removal is performed in the subsequent culture process, the yield will not be affected, which greatly saves manpower and material resources and effectively reduces production costs.

[0017] Light has a significant impact on the growth of sea grapes. If the light is too weak, the algae will grow slowly and appear bleached. If the light is too strong, benthic diatoms will reproduce and easily attach to the sea grape algae, thus affecting their growth. The present invention adjusts the seawater depth of the breeding pond and cultivates sea grapes in seawater 1.5 to 2 meters deep. The refraction of seawater can effectively reduce the impact of direct strong light, thereby promoting the growth of sea grapes and improving their quality.

[0018] Red light and blue light are the two most effective spectral regions in photosynthesis. Alternating red and blue light irradiation can ensure that sea grapes can carry out effective photosynthesis under different light qualities, promote energy conversion and the synthesis of organic matter, improve the energy utilization rate of sea grapes, and thereby increase the content of nutrients and optimize their quality.

[0019] By adjusting the type and amount of fertilizer, this method provides comprehensive nutrition for sea grape growth, promoting rapid and efficient reproduction, enhancing plant resistance, and ultimately improving sea grape quality and yield. By applying fertilizer in stages, the system ensures adequate nutrient supply throughout the sea grape's growth cycle. Furthermore, this rational fertilization strategy effectively avoids nutrient deficiencies or excesses, reducing associated losses and ultimately saving cultivation costs. DETAILED DESCRIPTION

[0020] To further illustrate the present invention, a method for artificially growing sea grapes provided by the present invention is described in detail below with reference to examples, but these examples should not be construed as limiting the scope of protection of the present invention.

[0021] Example 1 A method for artificially growing sea grapes, comprising the following steps: Seedling temporary rearing: After the temporary rearing pond is fully cleaned and exposed to the sun, fill it with seawater filtered through a 60-mesh filter at a height of 0.5m. Select healthy sea grapes with upright stems of 10-15cm in height. The sea grape variety is long-stem grape fern algae. After cleaning and removing impurities, sow them on the substrate and place them in the culture pond for temporary rearing for 7 days. The temporary rearing temperature is 23℃-30℃, the light source is natural light with a light intensity of 5000Lux, and the sowing amount is 600g / m 2 .

[0022] Stocking of seedlings: After the culture pond is disinfected with potassium permanganate, it is filled with seawater filtered through a 60-mesh filter at a height of 1.5m. The seedlings are removed from the temporary holding pond and stocked in the culture pond for breeding. The seeding rate is 1500g / m 2 The culture temperature was 25℃~28℃, and the irradiation was alternating between 30min red light and 30min blue light, with a light intensity of 5000Lux and a light-dark ratio of 16h / 8h. The first fertilization was carried out on the 10th day of culture, and the fertilizer dosage was: 15g / m3 of fermentation cake 2 , slaked lime 8g / m 2 , sodium hydrogen phosphate 8g / m 2 , dipotassium hydrogen phosphate 6g / m 2 , humic acid iron 6g / m 2 and ammonium ferric citrate 5g / m 2 On the 30th day of cultivation, the second fertilization was carried out, and the fertilizer dosage was: fermentation cake 25g / m 2 , slaked lime 8g / m 2 , sodium hydrogen phosphate 10g / m 2 , dipotassium hydrogen phosphate 8g / m 2 , humic acid iron 10g / m 2 and ammonium ferric citrate 6g / m 2 During the cultivation period, the algae were removed manually every 10 days.

[0023] Purification culture: After the purification pool is disinfected with potassium permanganate, artificial seawater with a salinity of 2.7% is injected. After the 90th day of cultivation, the sea grapes are transferred to the purification pool for purification culture; the temperature of the purification culture is 25°C~28°C, the light source is natural light, the light intensity is 5000 Lux, and the light-dark ratio is 12h / 12h.

[0024] Harvesting: Harvest sea grapes after 30 days of purification culture.

[0025] Example 2 A method for artificially growing sea grapes, comprising the following steps: Seedling temporary rearing: After the temporary rearing pond is fully cleaned and exposed to the sun, fill it with seawater filtered through a 60-mesh filter at a height of 0.5m. Select healthy sea grapes with upright stems of 10-15cm in height. The sea grape variety is long-stem grape fern algae. After cleaning and removing impurities, sow them on the substrate and place them in the culture pond for temporary rearing for 7 days. The temporary rearing temperature is 23℃-30℃, the light source is natural light with a light intensity of 6000Lux, and the sowing amount is 600g / m 2 .

[0026] Stocking of seedlings: After the culture pond is disinfected with potassium permanganate, it is filled with seawater filtered through a 60-mesh filter at a height of 1.5m. The seedlings are removed from the temporary holding pond and stocked in the culture pond for breeding. The seeding rate is 1500g / m 2 The culture temperature is 25℃~28℃, and the irradiation is alternating between 30min red light and 30min blue light, with a light intensity of 6000Lux and a light-dark ratio of 16h / 8h. On the 10th day of culture, the first fertilization is carried out, and the fertilizer dosage is: 15g / m3 of fermentation cake 2 , slaked lime 8g / m 2 , sodium hydrogen phosphate 8g / m 2 , dipotassium hydrogen phosphate 6g / m 2 , humic acid iron 6g / m 2 and ammonium ferric citrate 5g / m 2 On the 30th day of cultivation, the second fertilization was carried out, and the fertilizer dosage was: fermentation cake 25g / m 2 , slaked lime 8g / m 2 , sodium hydrogen phosphate 10g / m 2 , dipotassium hydrogen phosphate 8g / m 2 , humic acid iron 10g / m 2 and ammonium ferric citrate 6g / m 2 During the cultivation period, the algae were removed manually every 10 days.

[0027] Purification culture: After the purification pool is disinfected with potassium permanganate, artificial seawater with a salinity of 2.7% is injected. After the 90th day of cultivation, the sea grapes are transferred to the purification pool for purification culture; the temperature of the purification culture is 25℃~28℃, the light source is natural light, the light intensity is 6000Lux, and the light-dark ratio is 12h / 12h.

[0028] Harvesting: Harvest sea grapes after 30 days of purification culture.

[0029] Example 3 Seedling temporary rearing: After the temporary rearing pond is fully cleaned and exposed to the sun, fill it with seawater filtered through a 60-mesh filter at a height of 0.5m. Select healthy sea grapes with upright stems of 10-15cm in height. The sea grape variety is long-stem grape fern algae. After cleaning and removing impurities, sow them on the substrate and place them in the culture pond for temporary rearing for 7 days. The temporary rearing temperature is 23℃-30℃, the light source is natural light with a light intensity of 8000Lux, and the sowing amount is 600g / m 2 .

[0030] Stocking of seedlings: After the culture pond is disinfected with potassium permanganate, it is filled with seawater filtered through a 60-mesh filter at a height of 1.5m. The seedlings are removed from the temporary holding pond and stocked in the culture pond for breeding. The seeding rate is 1500g / m 2 The culture temperature is 25℃~28℃, and the irradiation is alternating between 30min red light and 30min blue light, with a light intensity of 8000Lux and a light-dark ratio of 16h / 8h. On the 10th day of culture, the first fertilization is carried out, and the fertilizer dosage is: 15g / m3 of fermentation cake 2 , slaked lime 8g / m 2 , sodium hydrogen phosphate 8g / m 2 , dipotassium hydrogen phosphate 6g / m 2 , humic acid iron 6g / m 2 and ammonium ferric citrate 5g / m 2 On the 30th day of cultivation, the second fertilization was carried out, and the fertilizer dosage was: fermentation cake 25g / m 2 , slaked lime 8g / m 2 , sodium hydrogen phosphate 10g / m 2 , dipotassium hydrogen phosphate 8g / m 2 , humic acid iron 10g / m 2 and ammonium ferric citrate 6g / m 2 During the cultivation period, the algae were removed manually every 10 days.

[0031] Purification culture: After the purification pool is disinfected with potassium permanganate, artificial seawater with a salinity of 2.7% is injected. After the 90th day of cultivation, the sea grapes are transferred to the purification pool for purification culture; the temperature of the purification culture is 25℃~28℃, the light source is natural light, the light intensity is 8000Lux, and the light-dark ratio is 12h / 12h.

[0032] Harvesting: Harvest sea grapes after 30 days of purification culture.

[0033] Example 4 A method for artificially growing sea grapes, comprising the following steps: Seedling temporary rearing: After the temporary rearing pond is fully cleaned and exposed to the sun, fill it with seawater filtered through a 60-mesh filter at a height of 0.5m. Select healthy sea grapes with upright stems of 10-15cm in height. The sea grape variety is long-stem grape fern algae. After cleaning and removing impurities, sow them on the substrate and place them in the culture pond for temporary rearing for 7 days. The temporary rearing temperature is 23℃-30℃, the light source is natural light with a light intensity of 8000Lux, and the sowing amount is 600g / m 2 .

[0034] Stocking of seedlings: After the culture pond is disinfected with potassium permanganate, it is filled with seawater filtered through a 60-mesh filter at a height of 1.5m. The seedlings are removed from the temporary holding pond and stocked in the culture pond for breeding. The seeding rate is 1500g / m 2 The culture temperature is 25℃~28℃, and the irradiation is alternating between 30min red light and 30min blue light, with a light intensity of 8000Lux and a light-dark ratio of 16h / 8h. On the 10th day of culture, the first fertilization is carried out, and the fertilizer dosage is: 15g / m3 of fermentation cake 2 , slaked lime 5g / m 2 , sodium hydrogen phosphate 4g / m 2 , dipotassium hydrogen phosphate 2g / m 2 , humic acid iron 3g / m 2 and ammonium ferric citrate 3g / m 2 On the 30th day of cultivation, the second fertilization was carried out, and the fertilizer dosage was: fermentation cake 20g / m 2 , slaked lime 5g / m 2 , sodium hydrogen phosphate 8g / m 2 , dipotassium hydrogen phosphate 5g / m 2 , humic acid iron 6g / m 2 and ammonium ferric citrate 5g / m 2 During the cultivation period, the algae were removed manually every 10 days.

[0035] Purification culture: After the purification pool is disinfected with potassium permanganate, artificial seawater with a salinity of 2.7% is injected. After the 90th day of cultivation, the sea grapes are transferred to the purification pool for purification culture; the temperature of the purification culture is 25℃~28℃, the light source is natural light, the light intensity is 8000Lux, and the light-dark ratio is 12h / 12h.

[0036] Harvesting: Harvest sea grapes after 30 days of purification culture.

[0037] Example 5 A method for artificially growing sea grapes, comprising the following steps: Seedling temporary rearing: After the temporary rearing pond is fully cleaned and exposed to the sun, fill it with seawater filtered through a 60-mesh filter at a height of 0.5m. Select healthy sea grapes with upright stems of 10-15cm in height. The sea grape variety is long-stem grape fern algae. After cleaning and removing impurities, sow them on the substrate and place them in the culture pond for temporary rearing for 7 days. The temporary rearing temperature is 23℃-30℃, the light source is natural light with a light intensity of 8000Lux, and the sowing amount is 600g / m 2 .

[0038] Stocking of seedlings: After the culture pond is disinfected with potassium permanganate, it is filled with seawater filtered through a 60-mesh filter at a height of 1.5m. The seedlings are removed from the temporary holding pond and stocked in the culture pond for breeding. The seeding rate is 1500g / m 2 The culture temperature is 25℃~28℃, and the irradiation is alternating between 30min red light and 30min blue light, with a light intensity of 8000Lux and a light-dark ratio of 16h / 8h. On the 10th day of culture, the first fertilization is carried out, and the fertilizer dosage is: 30g / m3 of fermentation cake 2 , slaked lime 10g / m 2 , sodium hydrogen phosphate 9g / m 2 , dipotassium hydrogen phosphate 6g / m 2 , humic acid iron 8g / m 2 and ammonium ferric citrate 5g / m 2 On the 30th day of cultivation, the second fertilization was carried out, and the fertilizer dosage was: fermentation cake 35g / m 2 , slaked lime 10g / m 2 , sodium hydrogen phosphate 12g / m 2 , dipotassium hydrogen phosphate 8g / m 2 , humic acid iron 10g / m 2 and ammonium ferric citrate 8g / m 2 During the cultivation period, the algae were removed manually every 10 days.

[0039] Purification culture: After the purification pool is disinfected with potassium permanganate, artificial seawater with a salinity of 2.7% is injected. After the 90th day of cultivation, the sea grapes are transferred to the purification pool for purification culture; the temperature of the purification culture is 25℃~28℃, the light source is natural light, the light intensity is 8000Lux, and the light-dark ratio is 12h / 12h.

[0040] Harvesting: Harvest sea grapes after 30 days of purification culture.

[0041] Example 6 A method for artificially growing sea grapes, comprising the following steps: Seedling temporary rearing: After the temporary rearing pond is fully cleaned and exposed to the sun, fill it with seawater filtered through a 60-mesh filter at a height of 0.5m. Select healthy sea grapes with upright stems of 10-15cm in height. The sea grape variety is long-stem grape fern algae. After cleaning and removing impurities, sow them on the substrate and place them in the culture pond for temporary rearing for 7 days. The temporary rearing temperature is 23℃-30℃, the light source is natural light with a light intensity of 8000Lux, and the sowing amount is 600g / m 2 .

[0042] Stocking of seedlings: After the culture pond is disinfected with potassium permanganate, it is filled with seawater filtered through a 60-mesh filter at a height of 1.5m. The seedlings are removed from the temporary holding pond and stocked in the culture pond for breeding. The seeding rate is 1500g / m 2 The culture temperature is 25℃~28℃, and the irradiation is alternating between 30min red light and 30min blue light, with a light intensity of 8000Lux and a light-dark ratio of 16h / 8h. On the 10th day of culture, the first fertilization is carried out, and the fertilizer dosage is: 15~30g / m 2 , slaked lime 5~10g / m 2 , sodium hydrogen phosphate 4~9g / m 2 , dipotassium hydrogen phosphate 2~6g / m 2 , humic acid iron 3~8g / m 2 and ammonium ferric citrate 3~5g / m 2 On the 30th day of cultivation, the second fertilization was carried out. The fertilizer dosage was: 20-35g / m2 of fermentation cake. 2 , slaked lime 5~10g / m 2 , sodium hydrogen phosphate 8~12g / m 2 , dipotassium hydrogen phosphate 5~8g / m 2 , humic acid iron 6~10g / m 2 and ammonium ferric citrate 5~8g / m 2 During the cultivation period, the algae were removed manually every 10 days.

[0043] Purification culture: After the purification pool is disinfected with potassium permanganate, artificial seawater with a salinity of 2.5% is injected. After the 90th day of cultivation, the sea grapes are transferred to the purification pool for purification culture; the temperature of the purification culture is 25°C~28°C, the light source is natural light, the light intensity is 8000 Lux, and the light-dark ratio is 12h / 12h.

[0044] Harvesting: Harvest sea grapes after 20 days of purification culture.

[0045] Example 7 A method for artificially growing sea grapes, comprising the following steps: Seedling temporary rearing: After the temporary rearing pond is fully cleaned and exposed to the sun, fill it with seawater filtered through a 60-mesh filter at a height of 0.5m. Select healthy sea grapes with upright stems of 10-15cm in height. The sea grape variety is long-stem grape fern algae. After cleaning and removing impurities, sow them on the substrate and place them in the culture pond for temporary rearing for 7 days. The temporary rearing temperature is 23℃-30℃, the light source is natural light with a light intensity of 8000Lux, and the sowing amount is 600g / m 2 .

[0046] Stocking of seedlings: After the culture pond is disinfected with potassium permanganate, it is filled with seawater filtered through a 60-mesh filter at a height of 1.5m. The seedlings are removed from the temporary holding pond and stocked in the culture pond for breeding. The seeding rate is 1500g / m 2 The culture temperature is 25℃~28℃, and the irradiation is alternating between 30min red light and 30min blue light, with a light intensity of 8000Lux and a light-dark ratio of 16h / 8h. On the 10th day of culture, the first fertilization is carried out, and the fertilizer dosage is: 15~30g / m 2 , slaked lime 5~10g / m 2 , sodium hydrogen phosphate 4~9g / m 2 , dipotassium hydrogen phosphate 2~6g / m 2 , humic acid iron 3~8g / m 2 and ammonium ferric citrate 3~5g / m 2 On the 30th day of cultivation, the second fertilization was carried out. The fertilizer dosage was: 20-35g / m2 of fermentation cake. 2 , slaked lime 5~10g / m 2 , sodium hydrogen phosphate 8~12g / m 2 , dipotassium hydrogen phosphate 5~8g / m 2 , humic acid iron 6~10g / m 2 and ammonium ferric citrate 5~8g / m 2 During the cultivation period, the algae were removed manually every 10 days.

[0047] Purification culture: After the purification pool is disinfected with potassium permanganate, artificial seawater with a salinity of 2.5% is injected. After the 90th day of cultivation, the sea grapes are transferred to the purification pool for purification culture; the temperature of the purification culture is 25°C~28°C, the light source is natural light, the light intensity is 8000 Lux, and the light-dark ratio is 12h / 12h.

[0048] Harvesting: Harvest sea grapes after 25 days of purification culture.

[0049] Comparative Example 1 A method for artificially growing sea grapes, comprising the following steps: Stocking: After disinfecting the pond with potassium permanganate, fill it with seawater filtered through a 60-mesh filter at a height of 1.5m. Select healthy sea grapes with upright stems of 10-15cm in height. The sea grape variety is long-stem grape fern algae. After cleaning and removing impurities, sow them on the substrate and plant them in the pond for cultivation. The sowing rate is 1500g / m 2 The culture temperature is 25℃~28℃, and the irradiation is alternating between 30min red light and 30min blue light, with a light intensity of 5000Lux and a light-dark ratio of 16h / 8h. On the 10th day of culture, the first fertilization is carried out, and the fertilizer dosage is: 20g / m3 of fermentation cake 2 , slaked lime 8g / m 2 , sodium hydrogen phosphate 5g / m 2 , dipotassium hydrogen phosphate 5g / m 2 , humic acid iron 5g / m 2 and ammonium ferric citrate 4g / m 2 On the 30th day of cultivation, the second fertilization was carried out, and the fertilizer dosage was: fermentation cake 30g / m 2 , slaked lime 8g / m 2 , sodium hydrogen phosphate 10g / m 2 , dipotassium hydrogen phosphate 6g / m 2 , humic acid iron 8g / m 2 and ammonium ferric citrate 6g / m 2 During the cultivation period, the algae were removed manually every 10 days.

[0050] Purification culture: After the purification pool is disinfected with potassium permanganate, artificial seawater with a salinity of 2.7% is injected. After the 90th day of cultivation, the sea grapes are transferred to the purification pool for purification culture; the temperature of the purification culture is 25°C~28°C, the light source is natural light, the light intensity is 5000 Lux, and the light-dark ratio is 12h / 12h.

[0051] Harvesting: Harvest sea grapes after 30 days of purification culture.

[0052] Comparative Example 2 A method for artificially growing sea grapes, comprising the following steps: Seedling temporary rearing: After the temporary rearing pond is fully cleaned and exposed to the sun, fill it with seawater filtered through a 60-mesh filter at a height of 0.5m. Select healthy sea grapes with upright stems of 10-15cm in height. The sea grape variety is long-stem grape fern algae. After cleaning and removing impurities, sow them on the substrate and place them in the culture pond for temporary rearing for 7 days. The temporary rearing temperature is 23℃-30℃, the light source is natural light with a light intensity of 5000Lux, and the sowing amount is 600g / m 2 .

[0053] Stocking: After disinfecting the pond with potassium permanganate, fill it with seawater filtered through a 60-mesh filter at a height of 1.5m. Select healthy sea grapes with upright stems of 10-15cm in height. The sea grape variety is long-stem grape fern algae. After cleaning and removing impurities, sow them on the substrate and plant them in the pond for cultivation. The sowing rate is 1500g / m 2 The culture temperature is 25℃~28℃, natural light is used, the light intensity is 5000Lux, and the light-dark ratio is 16h / 8h. On the 10th day of culture, the first fertilization is carried out, and the fertilizer dosage is: fermentation cake 15g / m 2 , slaked lime 8g / m 2 , sodium hydrogen phosphate 8g / m 2 , dipotassium hydrogen phosphate 6g / m 2 , humic acid iron 6g / m 2 and ammonium ferric citrate 5g / m 2 On the 30th day of cultivation, the second fertilization was carried out, and the fertilizer dosage was: fermentation cake 25g / m 2 , slaked lime 8g / m 2 , sodium hydrogen phosphate 10g / m 2 , dipotassium hydrogen phosphate 8g / m 2 , humic acid iron 10g / m 2 and ammonium ferric citrate 6g / m 2 During the cultivation period, the algae were removed manually every 10 days.

[0054] Purification culture: After the purification pool is disinfected with potassium permanganate, artificial seawater with a salinity of 2.7% is injected. After the 90th day of cultivation, the sea grapes are transferred to the purification pool for purification culture; the temperature of the purification culture is 25°C~28°C, the light source is natural light, the light intensity is 5000 Lux, and the light-dark ratio is 12h / 12h.

[0055] Harvesting: Harvest sea grapes after 30 days of purification culture.

[0056] Comparative Example 3 A method for artificially growing sea grapes, comprising the following steps: Seedling temporary rearing: After the temporary rearing pond is fully cleaned and exposed to the sun, fill it with seawater filtered through a 60-mesh filter at a height of 0.5m. Select healthy sea grapes with upright stems of 10-15cm in height. The sea grape variety is long-stem grape fern algae. After cleaning and removing impurities, sow them on the substrate and place them in the culture pond for temporary rearing for 7 days. The temporary rearing temperature is 23℃-30℃, the light source is natural light with a light intensity of 5000Lux, and the sowing amount is 600g / m 2 .

[0057] Stocking: After disinfecting the pond with potassium permanganate, fill it with seawater filtered through a 60-mesh filter at a height of 1.5m. Select healthy sea grapes with upright stems of 10-15cm in height. The sea grape variety is long-stem grape fern algae. After cleaning and removing impurities, sow them on the substrate and plant them in the pond for cultivation. The sowing rate is 1500g / m 2 The culture temperature is 25℃~28℃, and red and blue light are used simultaneously. The light intensity is 5000Lux and the light-dark ratio is 16h / 8h. On the 10th day of culture, the first fertilization is carried out. The fertilizer dosage is: 15g / m 2 , slaked lime 8g / m 2 , sodium hydrogen phosphate 8g / m 2 , dipotassium hydrogen phosphate 6g / m 2 , humic acid iron 6g / m 2 and ammonium ferric citrate 5g / m 2 On the 30th day of cultivation, the second fertilization was carried out, and the fertilizer dosage was: fermentation cake 25g / m 2 , slaked lime 8g / m 2 , sodium hydrogen phosphate 10g / m 2 , dipotassium hydrogen phosphate 8g / m 2 , humic acid iron 10g / m 2 and ammonium ferric citrate 6g / m 2 During the cultivation period, the algae were removed manually every 10 days.

[0058] Purification culture: After the purification pool is disinfected with potassium permanganate, artificial seawater with a salinity of 2.7% is injected. After the 90th day of cultivation, the sea grapes are transferred to the purification pool for purification culture; the temperature of the purification culture is 25°C~28°C, the light source is natural light, the light intensity is 5000 Lux, and the light-dark ratio is 12h / 12h.

[0059] Harvesting: Harvest sea grapes after 30 days of purification culture.

[0060] Comparative Example 4 A method for artificially growing sea grapes, comprising the following steps: Seedling temporary rearing: After the temporary rearing pond is fully cleaned and exposed to the sun, fill it with seawater filtered through a 60-mesh filter at a height of 0.5m. Select healthy sea grapes with upright stems of 10-15cm in height. The sea grape variety is long-stem grape fern algae. After cleaning and removing impurities, sow them on the substrate and place them in the culture pond for temporary rearing for 7 days. The temporary rearing temperature is 23℃-30℃, the light source is natural light with a light intensity of 5000Lux, and the sowing amount is 600g / m 2 .

[0061] Stocking: After disinfecting the pond with potassium permanganate, fill it with seawater filtered through a 60-mesh filter at a height of 1.5m. Select healthy sea grapes with upright stems of 10-15cm in height. The sea grape variety is long-stem grape fern algae. After cleaning and removing impurities, sow them on the substrate and plant them in the pond for cultivation. The sowing rate is 1500g / m 2 The culture temperature is 25℃~28℃, and the irradiation is alternating between 30min red light and 30min blue light, with a light intensity of 5000Lux and a light-dark ratio of 12h / 12h. On the 10th day of culture, the first fertilization is carried out, and the fertilizer dosage is: 15g / m 2 , slaked lime 8g / m 2 , sodium hydrogen phosphate 8g / m 2 , dipotassium hydrogen phosphate 6g / m 2 , humic acid iron 6g / m 2 and ammonium ferric citrate 5g / m 2 On the 30th day of cultivation, the second fertilization was carried out, and the fertilizer dosage was: fermentation cake 25g / m 2 , slaked lime 8g / m 2 , sodium hydrogen phosphate 10g / m 2 , dipotassium hydrogen phosphate 8g / m 2 , humic acid iron 10g / m 2 and ammonium ferric citrate 6g / m 2 During the cultivation period, the algae were removed manually every 10 days.

[0062] Purification culture: After the purification pool is disinfected with potassium permanganate, artificial seawater with a salinity of 2.7% is injected. After the 90th day of cultivation, the sea grapes are transferred to the purification pool for purification culture; the temperature of the purification culture is 25°C~28°C, the light source is natural light, the light intensity is 5000 Lux, and the light-dark ratio is 12h / 12h.

[0063] Harvesting: Harvest sea grapes after 30 days of purification culture.

[0064] Test Example 1 The survival rate, yield, polysaccharide content and crude protein content of the sea grapes of Examples 1 to 7 and Comparative Examples 1 to 4 were statistically analyzed, and the results are shown in Table 1.

[0065] Table 1 Production quality of sea grapes

[0066] As shown in Table 1, compared to Comparative Example 1, the sea grapes from Examples 1 to 7 had a generally higher survival rate, a generally lower morbidity rate, and a higher yield; this demonstrates that the temporary culture of sea grapes according to the present invention effectively improves their stress resistance. Compared to Comparative Examples 2 to 4, the sea grapes from Examples 1 to 7 had a higher yield, with significantly increased crude protein and polysaccharide content. This demonstrates that alternating red and blue light exposure and appropriately extended illumination time are beneficial for promoting sea grape growth and increasing their nutrient content. This demonstrates that the method provided by the present invention is simpler and more efficient.

[0067] Although the above embodiment provides a detailed description of the present invention, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the scope of protection of the present invention.

Claims

1. A method for artificially growing sea grapes, characterized in that: The method comprises the following steps: Seedling temporary rearing: After the temporary rearing pond is disinfected, it is filled with filtered seawater at a height of 0.5-1m. Healthy sea grapes with upright stems of 10-15cm are selected, cleaned, and impurities removed. After sowing, they are planted on the substrate and placed in the temporary rearing pond for 5-7 days. The sowing amount is 500-800g / m 2 The temporary culture temperature is 23 ℃ ~ 30 ℃, the light source is natural light, the light intensity is 5000 ~ 8000 Lux, and the light-dark ratio is 12h / 12h; Stocking of seedlings: After the culture pond is disinfected, it is filled with filtered seawater. The seedlings are taken out from the temporary holding pond and placed in the culture pond for cultivation. The seeding rate is 1500~2000g / m 2 ; When the culture is to the 10th day, the first fertilization is carried out, and when the culture is to the 30th day, the second fertilization is carried out; the culture temperature is 25 ℃ ~ 28 ℃, the light source is red and blue light, the red and blue light irradiation mode is 30 minutes red light / 30 minutes blue light alternating irradiation, the light intensity is 5000 ~ 8000 Lux, and the light-dark ratio is 16h / 8h; Purification culture: After disinfection, the purification pool is injected with artificial seawater with a salinity of 2.5% to 2.8%. On the 90th day of cultivation, the sea grapes are transferred to the purification pool for purification culture. The purification culture temperature is 25°C to 28°C, the light source is natural light, the light intensity is 5000-8000 Lux, and the light-dark ratio is 12h / 12h. Harvest: Harvest after 20 to 30 days of purification culture.

2. The method according to claim 1, characterized in that The disinfection method is sunlight exposure and / or potassium permanganate disinfection.

3. The method according to claim 1, characterized in that The pore size of the filtration is 60-80 mesh.

4. The method according to claim 1, wherein The species of sea grapes is long stem grape fern algae ( Caulerpa lentillifera ).

5. The method according to claim 1, characterized in that The attachment base is a polyethylene mesh cage.

6. The method according to claim 1, characterized in that The height of the seawater in the culture pond is 1.5-2 m.

7. The method according to claim 1, characterized in that During the cultivation period, the algae were manually removed every 10 days.

8. The method according to claim 1, characterized in that The fertilization method is to sprinkle fertilizer into the breeding pond.

9. The method according to claim 1, characterized in that The amount of fertilizer used for the first fertilization is: 15-30g / m 2 , slaked lime 5~10g / m 2 , sodium hydrogen phosphate 4~9g / m 2 , dipotassium hydrogen phosphate 2~6g / m 2 , humic acid iron 3~8g / m 2 and ammonium ferric citrate 3~5g / m 2 .

10. The method according to claim 1, characterized in that The fertilizer dosage for the second fertilization is: 20~35g / m 2 , slaked lime 5~10g / m 2 , sodium hydrogen phosphate 8~12g / m 2 , dipotassium hydrogen phosphate 5~8g / m 2 , humic acid iron 6~10g / m 2 and ammonium ferric citrate 5~8g / m 2 .

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