In-situ ecological treatment method for recycling prawn culture tail water and application of in-situ ecological treatment method

Abstract

The invention provides an in-situ ecological treatment method for recycling prawn culture tail water and application of the in-situ ecological treatment method, and belongs to the technical field of aquaculture wastewater treatment. After the miscellaneous shrimps in the culture water body are cleaned, quick lime is splashed to be matched with an iron chain to drag the bottom, and then a composite treating agent is put; inoculating rotifer, and then supplementing mineral substances; performing water quality verification; the composite treatment agent comprises EM bacteria, a seawater chlorella stock solution and a carbon source. According to the method, in-situ ecological treatment of prawn culture tail water and a zero-drainage culture mode are achieved, the treatment period is short, the culture survival rate is high, new treatment facilities are not needed, and beneficial bacterial algal phases and nutrient elements can be reserved.

Description

Technical Field

[0001] The invention belongs to the technical field of aquaculture wastewater treatment, and particularly relates to an in-situ ecological treatment method for recycling shrimp aquaculture tail water and an application thereof. Background Art

[0002] Shrimp aquaculture tailwater contains large amounts of nutrients like nitrogen and phosphorus, organic matter, suspended solids, and pollutants like residual pharmaceuticals and disinfectants. If discharged without effective treatment, it can lead to eutrophication, causing algae overgrowth and impacting the living environment of surrounding aquatic life. Furthermore, the accumulation of pollutants can cause black and odorous water and reduced dissolved oxygen levels.

[0003] The current technical bottlenecks in shrimp aquaculture tailwater treatment are as follows: 1) The traditional three-pond, two-embankment method requires an additional 30-40% of land, increasing infrastructure costs by more than 25%; 2) Chemical disinfection destroys beneficial bacteria and algae, requiring re-cultivation of the water for each breeding crop; 3) External treatment carries the risk of secondary pollution and a nutrient loss rate of 60-70%; 4) Existing circulating water systems consume as much as 2.5-3.5kW·h / m 3 , the operating cost is high. At present, the development of an in-situ ecological treatment method for the recycling of shrimp aquaculture tail water without external discharge, short treatment cycle and high aquaculture survival rate is a technical problem that needs to be solved at this stage. Summary of the Invention

[0004] In view of this, the purpose of the present invention is to provide an in situ ecological treatment method for the recycling of shrimp aquaculture tail water and its application. This method realizes in situ ecological treatment of tail water, has a short treatment cycle, a high aquaculture survival rate, and does not require the construction of new treatment facilities, and can retain beneficial bacteria and algae and nutrients.

[0005] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0006] The invention provides an in-situ ecological treatment method for recycling shrimp aquaculture tail water, comprising the following steps: cleaning the aquaculture water body of miscellaneous shrimps, splashing quicklime with iron chains to drag the bottom, and then adding a composite treatment agent; inoculating rotifers and then supplementing minerals; and verifying the water quality; the composite treatment agent comprises EM bacterial liquid, seawater chlorella stock solution and a carbon source.

[0007] Preferably, an electric shrimp net is used to clean the miscellaneous shrimps in the aquaculture water, and the operation frequency is ≥3 times; the voltage of the electric shrimp net is 12-24V.

[0008] Preferably, the amount of quicklime is 30-80g / m 3 .

[0009] Preferably, the chain drags the bottom 5-10 times.

[0010] Preferably, the dosage of the EM bacterial solution is 5-10 g / m 3 The dosage of the seawater chlorella stock solution is 2-5g / m 3 The amount of the carbon source is 10-50 g / m 3 .

[0011] Preferably, the carbon source comes from one or more of fruit enzymes fermented from apples or cherries, brown sugar, syrup, and molasses; the preparation steps of the fruit enzymes fermented from apples or cherries include: mixing apples or cherries, sugar and water in a weight ratio of 1:0.03-0.05:1-3, and fermenting at 20-30°C for 8-12 days.

[0012] Preferably, the inoculation amount of the rotifer is 1-5 g / m 3 .

[0013] Preferably, the minerals include sodium chloride, calcium magnesium salts, sodium bicarbonate and trace elements; after mineral supplementation, the water body controls salinity ≥5, the calcium magnesium mass ratio is 1:2-1:3, the total alkalinity is 120-150 mg / L, and the total hardness as CaCO3 is 500-1000 mg / L.

[0014] Preferably, the results of the water quality verification include: ammonia nitrogen ≤ 0.6 mg / L, nitrite ≤ 0.2 mg / L.

[0015] The present invention also provides application of the in-situ ecological treatment method in treating shrimp aquaculture tail water.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] The present invention adopts a six-step process of electric capture to remove impure shrimp → quicklime to activate bottom mud → composite bacteria and algae coordinated purification → rotifer biological regulation → mineral dynamic balance → water quality compliance verification. On the basis of the coordinated treatment of "physical impurities removal-chemical activation-biological purification", through the targeted cultivation of rotifers and the balance of water minerals, the environment required for shrimp growth is quickly rebuilt, and the in-situ ecological treatment of tailwater and zero-discharge aquaculture are achieved. Compared with traditional methods, the treatment cycle is shortened by 57%, the aquaculture survival rate is increased by 16.7%, and there is no need for new treatment facilities. Beneficial bacteria and algae phases and nutrients can be retained. By cultivating biological baits such as rotifers, the survival rate of shrimp fry is increased to more than 76.3%. The method described in the present invention is applicable to various aquaculture modes such as seawater / freshwater ponds and small sheds. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 Multi-layer insulation shed for shrimp farming.

[0019] Figure 2 This is a shrimp breeding pond after adding fruit enzymes.

[0020] Figure 3 These are the aquaculture ponds that have just been stocked with shrimp after being treated using the in situ ecological treatment method (the pond on the left of the picture) and the aquaculture ponds that have just been used to catch shrimp but have not undergone tail water treatment (the pond on the right of the picture). DETAILED DESCRIPTION

[0021] The present invention provides an in-situ ecological treatment method for recycling shrimp aquaculture tailwater, comprising the following steps: cleaning the aquaculture water of debris from shrimp, then spraying quicklime with iron chains to drag the bottom, and then adding a composite treatment agent; inoculating rotifers, and then supplementing with minerals; and verifying the water quality; the composite treatment agent comprises an EM bacterial solution, a marine Chlorella stock solution, and a carbon source. Cleaning the aquaculture water of debris from shrimp in the present invention is a physical cleaning step, which must ensure that the entire pond is clean and free of shrimp. Spraying quicklime with iron chains to drag the bottom is intended to promote the activation of bottom mud. Adding the composite treatment agent is intended to achieve the purpose of biological composite water purification. Inoculating rotifers is intended to achieve biological regulation of the water body. Supplementing minerals is intended to adjust the mineral balance of the water body.

[0022] In the present invention, an electric shrimp net is used to clean the aquaculture water of miscellaneous shrimps, with an operation frequency of ≥ 3 times, preferably 4 times, 5 times, 6 times, 7 times or 8 times; the voltage of the electric shrimp net is 12-24V and the power used by the electric shrimp net of the present invention is not less than 300W.

[0023] In the present invention, the amount of quicklime is 30-80g / m 3 , preferably 35-75g / m 3 , more preferably 50-70g / m 3 The number of times the iron chain is dragged on the bottom of the present invention is 5-10 times, preferably 6-9 times, and more preferably 7-8 times. The present invention sprinkles quicklime to maintain the pH of the water at 8.5-9.0 for 58-75 hours, and cooperates with the iron chain to drag on the bottom. The present invention loosens the bottom mud of the aquaculture pond by sprinkling lime, releases nutrients at the bottom of the pond, and reduces bottom pollution; the working principle of quicklime is: quicklime sprinkling produces Ca(OH)2 colloid, which promotes the flocculation and sedimentation of suspended matter through charge neutralization, and at the same time, Ca 2+ PO4 in sediment 3- Hydroxyapatite is generated to achieve phosphorus fixation. The present invention uses an iron chain to drag back and forth on the bottom of the pool to help turn over the bottom mud.

[0024] In the present invention, the dosage of the EM bacterial solution is 5-10 g / m 3 The dosage of the seawater chlorella stock solution is 2-5g / m 3 The amount of the carbon source is 10-50 g / m 3 As an embodiment, the dosage of the EM bacterial solution is preferably 6-9 g / m 3The dosage of the seawater chlorella stock solution is preferably 2.5-4.5 g / m 3 The amount of the carbon source is preferably 11-49 g / m 3 As another possible embodiment, the dosage of the EM bacterial solution is further preferably 7-8 g / m 3 The dosage of the seawater chlorella stock solution is further preferably 3-4 g / m 3 The amount of the carbon source is further preferably 20-30 g / m 3 . The EM bacterial liquid and seawater Chlorella stock solution described in the present invention can be purchased through commercial channels unless otherwise specified; the EM bacterial liquid is a composite bacterial agent mainly composed of more than 80 species of beneficial microorganisms from 10 genera, including photosynthetic bacteria, lactic acid bacteria, yeast, Bacillus, actinomycetes, acetic acid bacteria, filamentous fungi and bifidobacteria, and is preferably sourced from Zhengzhou Nongshengle Biological Co., Ltd.; the seawater Chlorella stock solution is preferably sourced from Binzhou Jingwei Bioengineering Co., Ltd. The carbon source described in the present invention is derived from one or more of fruit enzymes fermented from apples or cherries, brown sugar, syrup, and molasses, and is preferably derived from fruit enzymes fermented from apples or cherries. The preparation steps of the fruit enzyme fermented from apples or cherries of the present invention include: mixing apples or cherries, sugar and water in a weight ratio of 1:0.03-0.05:1-3, and fermenting at 20-30°C for 8-12 days; the weight ratio of apples or cherries, sugar and water is preferably 1:0.035-0.045:1.5-2.5, more preferably 1:0.04:2; the fermentation temperature is preferably 22-28°C, more preferably 25°C; the fermentation time is preferably 9-11 days, more preferably 10 days. The inoculated composite treatment agent of the present invention can deeply and comprehensively clean pollutants and improve the activity of water bodies and sludge.

[0025] In the present invention, the inoculation amount of the rotifer is 1-5 g / m 3 , preferably 1.5-4.5g / m 3 , more preferably 3-4g / m 3 The present invention not only reduces the content of debris and organic matter in the water and controls the growth of algae by directing the cultivation of rotifers in the water, but also converts them into biological bait for shrimp fry. The process of obtaining rotifers in the present invention includes: cleaning the indoor circular PP breeding pond, adding clean fresh water, adding 20-25 kg of seawater salt per cubic meter, fully aerating, and adding 3-7 g / m3 of worm eggs when the water temperature reaches 25±2°C. 3 , the first investment is 10-15g / m 3 The seawater chlorella stock solution (Binzhou Jingwei Bioengineering Co., Ltd.) was incubated at a salinity of 22-27 and a water temperature of 22-30°C. After 2-3 days of incubation, 10-15 g / m3 of seawater chlorella stock solution was added. 3 , and then every day according to 28-32% (g / m3 ) Add seawater Chlorella stock solution, after 7-10 days of cultivation, when 58-62% of the rotifers reach egg-bearing state, inoculate them into the culture pond. The rotifer eggs of the present invention are sourced from Binzhou Jingwei Bioengineering Co., Ltd.

[0026] In the present invention, the minerals include sodium chloride, calcium magnesium salts, sodium bicarbonate and trace elements; after the minerals are supplemented, the water body controls the salinity ≥ 5, the calcium magnesium mass ratio is 1:2-1:3, the total alkalinity is 120-150 mg / L, and the total hardness is 500-1000 mg / L in terms of CaCO3. The calcium magnesium salts described in the present invention are preferably calcium chloride and magnesium chloride; the trace elements include boric acid, sodium bromide and Antarctic krill powder (rich in trace elements such as iron, zinc, manganese, copper, selenium, etc., Zhuhai Produo Aquatic Technology Co., Ltd.). The amount of sodium chloride added in the shrimp aquaculture tail water treatment of the present invention is 0.3-4 kg / m 3 , preferably 0.4-2kg / m 3、 , more preferably 0.5-1kg / m 3 ; The amount of calcium chloride added is 0.03-0.15kg / m 3 , preferably 0.04-0.1kg / m 3 , more preferably 0.05-0.08kg / m 3 ; The amount of magnesium chloride added is 0.1-1.2kg / m 3 , preferably 0.2-1.0kg / m 3 , more preferably 0.3-0.7kg / m 3 ; The amount of sodium bicarbonate added is 0.02-0.1kg / m 3 , preferably 0.03-0.08kg / m 3 , more preferably 0.04-0.05kg / m 3 Among the trace elements of the present invention, the amount of boric acid added is 0.008-0.03 kg / m 3 , preferably 0.009-0.025kg / m 3 , more preferably 0.01-0.02kg / m 3 ; The amount of sodium bromide added is 0.002-0.006kg / m 3 , preferably 0.003-0.005kg / m 3 , more preferably 0.004-0.0045kg / m 3 ; The amount of Antarctic krill powder added is 0.01-0.08kg / m 3 , preferably 0.02-0.06kg / m 3 , more preferably 0.03-0.04kg / m 3In the present invention, the results of the water quality verification include: ammonia nitrogen ≤ 0.6 mg / L, nitrite ≤ 0.2 mg / L. After the water quality is verified to be qualified, the shrimp fry are tested for 46-50 hours and the survival rate reaches more than 90-98%. If the shrimp fry test is normal, the shrimp fry are released in time.

[0027] The present invention also provides the application of the in-situ ecological treatment method in treating shrimp aquaculture tail water. The in-situ ecological treatment method of the present invention is applicable to various aquaculture modes such as seawater / freshwater ponds and small sheds.

[0028] In the present invention, unless otherwise specified, all components or reagents are commercially available products well known to those skilled in the art.

[0029] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions of the present invention. Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0030] The experimental process of the following embodiment was carried out in a multi-layer insulation shed for shrimp farming ( Figure 1 ) is carried out in

[0031] Example 1

[0032] (1) Physical cleaning (removing foreign shrimp from the aquaculture water): On the first day, after the previous crop of shrimps are harvested and put on the market, use an electric shrimp net to clean the entire pond from foreign shrimps. When using the electric shrimp net, use an electric shrimp machine with a power of no less than 300W, matched with a 24V battery, and scrape the net over the entire pond to catch shrimps five times to ensure that all the shrimps in the pond are cleaned.

[0033] (2) Bottom mud activation: On the third day, press 70g / m 3 Sprinkle quicklime to maintain pH 9.0 for 48 hours, and drag the bottom of the pond with an iron chain 8 times: By spreading lime, the bottom mud of the breeding pond can be loosened, the nutrients at the bottom of the pond can be released, and the bottom pollution can be reduced.

[0034] (3) Composite purification: On the 5th day, EM bacterial solution 7g / m 3 , Seawater Chlorella stock solution 3g / m 3 and carbon source 30g / m 3 Composite treatment agent. EM bacterial solution (Effective Microorganisms) was sourced from Zhengzhou Nongshengle Biological Co., Ltd. The marine Chlorella stock solution was sourced from Binzhou Jingwei Bioengineering Co., Ltd. Preparation of carbon source: Cherries and water were mixed in a weight ratio of 1:2, and brown sugar was added at 4% (w / w) of the fruit weight. The mixture was sealed and fermented at 25°C for 9 days to obtain fruit enzyme, which is the carbon source. The state of the pool after adding the fruit enzyme in this process is shown in Figure 2 .

[0035] (4) Biological control: On the 7th day, inoculate 4g / m 3 Rotifers are used for ecological regulation. By cultivating rotifers in water bodies, not only can the content of debris and organic matter in the water be reduced and the growth of algae be controlled, but they can also be converted into biological bait for shrimp fry.

[0036] The rotifers used were obtained as follows: clean the indoor circular PP culture pond, add clean fresh water, add 23 kg of seawater salt per cubic meter, aerate thoroughly, and add 5 g / m2 of worm eggs (Binzhou Jingwei Bioengineering Co., Ltd.) when the water temperature reaches 25 ± 2 °C. 3 , the first investment is 13g / m 3 The seawater Chlorella stock solution (Binzhou Jingwei Bioengineering Co., Ltd.) was incubated at a salinity of 22-27 and a water temperature of 25±2°C. After two days of incubation, 13 g / m3 of seawater Chlorella stock solution was added. 3 , and then every day according to 30% of the water body (g / m 3 ) Add seawater Chlorella stock solution, and after 8 days of cultivation, when 60% or more of the rotifers reach the stage of spawning, inoculate them into the culture pond for biological control.

[0037] (5) Mineral balance: On the 8th day, add 0.5kg / m 3 Sodium chloride, 0.05kg / m 3 Calcium chloride, 0.4kg / m 3 Magnesium chloride, 0.04kg / m 3 Sodium bicarbonate, and 0.01kg / m 3 Boric acid, 0.004kg / m 3 Sodium bromide and 0.03kg / m 3 Antarctic krill meal, control water salinity ≥5, calcium-magnesium mass ratio of 1:2-1:3, total alkalinity 120-150mg / L, total hardness 500-1000mg / L (calculated as CaCO3).

[0038] (6) Water quality verification: After 8 days of treatment, the ammonia nitrogen in the water was detected to be ≤0.6mg / L and the nitrite was ≤0.2mg / L. After 48 hours of water testing, the survival rate of shrimp fry reached more than 95%. The shrimp fry water test was normal and the fry were released in time.

[0039] In this embodiment, the aquaculture pond just after stocking the seedlings after being treated by the in situ ecological treatment method is shown in FIG. Figure 3 The pond on the left is a breeding pond where the shrimps have just been caught but the tail water has not been treated. Figure 3 The pool on the right.

[0040] Example 2

[0041] (1) Physical cleaning (removing foreign shrimp from the aquaculture water): On the first day, after the previous crop of shrimps are harvested and put on the market, use an electric shrimp net to clean the entire pond from foreign shrimps. When using the electric shrimp net, use an electric shrimp machine with a power of no less than 300W and a 12V battery. Scrape the net over the entire pond and catch shrimp three times to ensure that all the shrimp in the pond are clean.

[0042] (2) Bottom mud activation: On the third day, press 30g / m 3 Sprinkle quicklime to maintain pH 9.0 for 48 hours, and drag the bottom of the pond with an iron chain 5 times: By spreading lime, the bottom mud of the breeding pond can be loosened, the nutrients at the bottom of the pond can be released, and the bottom pollution can be reduced.

[0043] (3) Composite purification: On the 5th day, add EM bacteria 5g / m 3 , Seawater Chlorella stock solution 2g / m 3 and carbon source 10g / m 3 Composite treatment agent. EM bacterial solution (Effective Microorganisms) was sourced from Zhengzhou Nongshengle Biological Co., Ltd. Marine Chlorella stock solution was sourced from Binzhou Jingwei Bioengineering Co., Ltd. Carbon source preparation: Cherries and water were mixed in a 1:1 weight ratio, and sugar was added at 3% (w / w) of the fruit weight. Fermented in a sealed container at 20°C for 8 days to obtain fruit enzyme, which served as the carbon source.

[0044] (4) Biological control: On the 7th day, inoculate 2g / m 3 Rotifers are used for ecological regulation. By cultivating rotifers in water bodies, not only can the content of debris and organic matter in the water be reduced and the growth of algae be controlled, but they can also be converted into biological bait for shrimp fry.

[0045] The rotifers used were obtained as follows: clean the indoor circular PP culture pond, add clean fresh water, add 20 kg of seawater salt per cubic meter, fully aerate, and put 3 g / m3 of worm eggs (Binzhou Jingwei Bioengineering Co., Ltd.) into the indoor water when the water temperature reaches 25 ± 2 °C. 3 , the first investment is 10g / m 3 The seawater Chlorella stock solution (Binzhou Jingwei Bioengineering Co., Ltd.) was incubated at a salinity of 22-27 and a water temperature of 21±1°C. After 3 days of incubation, 10 g / m3 of seawater Chlorella stock solution was added. 3 , and then every day according to 28% (g / m 3 ) Add seawater Chlorella stock solution, and after 7 days of cultivation, when 60% or more rotifers reach the stage of spawning, inoculate them into the culture pond for biological control.

[0046] (5) Mineral balance: On the 8th day, add 0.3kg / m 3 Sodium chloride, 0.03kg / m 3 Calcium chloride, 0.2kg / m 3Magnesium chloride, 0.02kg / m 3 Sodium bicarbonate, and 0.008kg / m 3 Boric acid, 0.002kg / m 3 Sodium bromide and 0.01kg / m 3 Antarctic krill meal, control water salinity ≥5, calcium-magnesium mass ratio of 1:2-1:3, total alkalinity 120-150mg / L, total hardness 500-1000mg / L (calculated as CaCO3).

[0047] (6) Water quality verification: After 8 days of treatment, the ammonia nitrogen in the water was detected to be ≤0.6mg / L and the nitrite was ≤0.2mg / L. After 48 hours of water testing, the survival rate of shrimp fry reached more than 95%. The shrimp fry water test was normal and the fry were released in time.

[0048] Example 3

[0049] (1) Physical cleaning (removing foreign shrimp from the aquaculture water): On the first day, after the previous crop of shrimps are harvested and put on the market, use an electric shrimp net to clean the entire pond from foreign shrimps. When using the electric shrimp net, use an electric shrimp machine with a power of no less than 300W, matched with a 24V battery, and scrape the net over the entire pond to catch shrimp three times to ensure that all the shrimp in the pond are clean.

[0050] (2) Bottom mud activation: On the third day, press 80g / m 3 Sprinkle quicklime to maintain pH 9.0 for 48 hours, and drag the bottom with an iron chain 10 times: By spreading lime, the bottom mud of the breeding pond can be loosened, the nutrients at the bottom of the pond can be released, and the bottom pollution can be reduced.

[0051] (3) Composite purification: On the 5th day, EM bacteria 10g / m 3 , Seawater Chlorella stock solution 5g / m 3 and carbon source 50g / m 3 Composite treatment agent. EM bacterial solution (Effective Microorganisms) was purchased from Zhengzhou Nongshengle Biological Co., Ltd. Marine Chlorella stock solution was sourced from Binzhou Jingwei Bioengineering Co., Ltd. Carbon source preparation: Cherries and water were mixed at a ratio of 1:3 by weight, and sugar was added at a ratio of 5% by weight to the fruit. The mixture was sealed and fermented at 30°C for 10 days to obtain fruit enzyme, which served as the carbon source.

[0052] (4) Biological control: On the 7th day, inoculate 5g / m 3 Rotifers are used for ecological regulation. By cultivating rotifers in water bodies, not only can the content of debris and organic matter in the water be reduced and the growth of algae be controlled, but they can also be converted into biological bait for shrimp fry.

[0053] The rotifers used were obtained as follows: clean the indoor circular PP culture pond, add clean fresh water, add 25 kg of seawater salt per cubic meter, aerate thoroughly, and add 7 g / m2 of worm eggs (Binzhou Jingwei Bioengineering Co., Ltd.) when the water temperature reaches 25 ± 2 °C. 3 , the first investment is 15g / m 3 The seawater Chlorella stock solution (Binzhou Jingwei Bioengineering Co., Ltd.) was incubated at a salinity of 22-27 and a water temperature of 27±1°C. After two days of incubation, 15 g / m3 of seawater Chlorella stock solution was added. 3 , and then every day according to 32% (g / m 3 ) Add seawater Chlorella stock solution, and after 10 days of cultivation, when 62% or more rotifers reach the stage of spawning, inoculate them into the culture pond for biological control.

[0054] (5) Mineral balance: On the 8th day, add 1kg / m 3 Sodium chloride, 0.1kg / m 3 Calcium chloride, 0.7kg / m 3 Magnesium chloride, 0.08kg / m 3 Sodium bicarbonate, and 0.02kg / m 3 Boric acid, 0.006kg / m 3 Sodium bromide and 0.06kg / m 3 Antarctic krill meal, control water salinity ≥5, calcium-magnesium mass ratio of 1:2-1:3, total alkalinity 120-150mg / L, total hardness 500-1000mg / L (calculated as CaCO3).

[0055] (6) Water quality verification: After 8 days of treatment, the ammonia nitrogen in the water was detected to be ≤0.6mg / L and the nitrite was ≤0.2mg / L. After 48 hours of water testing, the survival rate of shrimp fry reached more than 95%. The shrimp fry water test was normal and the fry were released in time.

[0056] Example 4

[0057] On March 26, 2024, the breeding experiment started in the No. 1-4 breeding sheds ( Figure 1 ) are stocked with shrimp fry. The area of ​​the small shed is 200 square meters (40 meters long, 5 meters wide and 1.2 meters deep). The bottom of the pond is paved with high-density polyethylene (HDPE) and the pond is insulated with three-layer polyethylene film (PVC).

[0058] 1. Preparation process before stocking: water was added uniformly on March 1 (water temperature 5℃). When the water temperature reached 18℃ on March 8, 40kg of lime was added to each pond and the pH value reached 11.2. On March 15, fruit enzyme (cherries and water were mixed in a weight ratio of 1:2, and brown sugar was added according to 4% (w / w) of the cherry weight. The fruit enzyme was fermented at 25℃ for 9 days to obtain the fruit enzyme) and the water pH was adjusted to 8.0. Then, 1500kg of mineral salt, 600kg of magnesium chloride, 20kg of potassium chloride, 10kg of boric acid, 2kg of potassium bromide, 50kg of sodium bicarbonate and 10kg of sodium chloride were added to each pond. Trace elements (0.8kg of sodium bromide and 6kg of Antarctic krill meal); on March 20, 3kg of EDTA was sprayed in each pond; on March 22, the water temperature reached 25℃, 2.5kg of EM bacterial solution (Zhengzhou Nongshengle Biological Co., Ltd.) and 10kg of brown sugar were sprayed in each pond; the water quality test results were pH 7.8-8.0, salinity 5-6, total alkalinity 120-130mg / L, total hardness 700-780mg / L, calcium ions 110-120mg / L, and magnesium ions 200-250mg / L, and then the water was tested with shrimp fry, and it was normal after 48 hours.

[0059] 2. Stocking: On March 26, 60,000 shrimp fry with a size of 0.8-1.0 cm were released into each pond.

[0060] 3. Daily management: In addition to normal feeding management every day, monitor water quality indicators twice a week, and add calcium chloride, magnesium chloride and sodium bicarbonate once every 10-15 days according to the water quality to adjust the water quality; when ammonia nitrogen ≥ 0.6 mg / L or nitrite ≥ 0.15 mg / L, promptly spray EM bacteria liquid + brown sugar + sea salt to adjust the water quality and reduce the ammonia nitrogen and nitrite content in the aquaculture water.

[0061] 4. Harvesting: By July 5th, when the shrimp fry have grown to 60-80 per kg, they are harvested and marketed. Each pond yields 490-525 kg of shrimp, with an average survival rate of 62.2%.

[0062] 5. Preparation for the second round of shrimp farming: On July 12, the shrimps in the four ponds were harvested and preparations for the next round of farming began.

[0063] Ponds 1-2 were treated ecologically using the method described in Example 1. After the first 7 days of treatment, water quality indicators were tested on the 8th day. The water quality indicators of ponds 1 and 2 were as follows: water temperature 28.2°C and 28.3°C, pH 8.1, salinity 5.5 and 5.8, total alkalinity 128mg / L and 120mg / L, total hardness 915mg / L and 893mg / L, calcium ion 120mg / L and 132mg / L, magnesium ion 175mg / L and 180mg / L, ammonia nitrogen and nitrite were both 0. To increase the magnesium ion content and optimize the optimal calcium-magnesium ion ratio, 50kg and 60kg of magnesium chloride were added to ponds 1-2, respectively. Shrimp fry water testing began on the 8th day. After 48 hours of shrimp fry water testing, the survival rate reached more than 95%. The shrimp fry water testing was normal. On the 10th day, July 23, 60,000 shrimp fry were released into each pond.

[0064] Pond No. 3-4 was drained (discharged to the base tailwater pool) and cleaned in a conventional manner, and then refilled with water. The water was cultivated according to the conventional process of step 1 of this embodiment. After 23 days of preparation, when the water temperature reached 26°C and the water quality was normal, shrimp fry were added, 60,000 per pond.

[0065] 6. Second batch of shrimp farming management: The farming management process refers to the first batch.

[0066] 7. Comparison of the second shrimp harvest: On October 16, after 86 days of culture, shrimp from Ponds 1 and 2 reached a size of 60 and 64 per kg, respectively, and began to be sold. Ponds 1 and 2 harvested 750 kg and 728 kg of shrimp, respectively, with survival rates of 75% and 77.7%. On November 9, after 98 days of culture, when shrimp sizes reached 64 and 68 per kg, Ponds 3 and 4 began to harvest and market shrimp. Ponds 3 and 4 harvested 587 kg and 602 kg of shrimp, respectively, with survival rates of 62.6% and 68.2%.

[0067] 8. Comparison of the two treatment methods: In the ponds (No. 1 and No. 2) using the original pond ecological treatment of the present invention, the time for the second shrimp farming was about 10 days, the breeding cycle was 86 days, the average size reached 62 tails / kg, and the average survival rate was 76.35%; in the ponds (No. 3 and No. 4) using the normal breeding method, the time for the second shrimp farming was about 23 days, the breeding cycle was 98 days, the average size reached 66 tails / kg, and the average survival rate was 65.4%. Comparing the results of the two aquaculture methods, shrimp cultured in ponds using ecological treatment of the original ponds grew faster, had higher survival rates and efficiency. The main reason for this is that the ecological treatment of the aquaculture tailwater in the original ponds quickly and comprehensively purifies the water quality and bottom sediments while retaining the excellent bacterial and algal phases of the original ponds, as well as the minerals and trace elements required for shrimp growth, to the greatest extent possible. This is conducive to improving the conversion and circulation of "nitrogen" and other elements during the aquaculture process, thereby providing a safer growth environment for shrimp more quickly. In addition, measures such as inoculating rotifers not only purify the water quality but also provide nutritious zooplankton for the early growth of shrimp fry, thereby improving the survival rate of shrimp fry. At the same time, because fewer mineral elements need to be supplemented during the aquaculture process, the overall aquaculture cost is lower.

[0068] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. An in-situ ecological treatment method for recycling shrimp aquaculture tail water, characterized in that: The process includes the following steps: cleaning the aquaculture water of miscellaneous shrimps, spraying quicklime with iron chains to drag the bottom, and then adding a compound treatment agent; inoculating rotifers, and then supplementing minerals; and verifying the water quality; The composite treatment agent comprises EM bacterial solution, seawater chlorella stock solution and a carbon source.

2. The in situ ecological treatment method according to claim 1, characterized in that: Use an electric shrimp net to clean the miscellaneous shrimps in the aquaculture water, with an operation frequency of ≥3 times; the voltage of the electric shrimp net is 12-24V.

3. The in situ ecological treatment method according to claim 1, characterized in that: The amount of quicklime is 30-80g / m 3 .

4. The in-situ ecological treatment method according to claim 1, characterized in that: The iron chain drags the bottom 5-10 times.

5. The in-situ ecological treatment method according to claim 1, characterized in that: The dosage of the EM bacterial solution is 5-10 g / m 3 The dosage of the seawater chlorella stock solution is 2-5g / m 3 The amount of the carbon source is 10-50 g / m 3 .

6. The in situ ecological treatment method according to claim 1 or 5, characterized in that: The carbon source is derived from one or more of fruit enzymes fermented from apples or cherries, brown sugar, syrup, and molasses; The preparation steps of the apple or cherry fermented fruit enzyme include: mixing apples or cherries, sugar and water in a weight ratio of 1:0.03-0.05:1-3, and fermenting at 20-30° C. for 8-12 days.

7. The in situ ecological treatment method according to claim 1, characterized in that: The inoculation amount of the rotifer is 1-5 g / m 3 .

8. The in-situ ecological treatment method according to claim 1, characterized in that: The minerals include sodium chloride, calcium magnesium salts, sodium bicarbonate and trace elements; after the minerals are supplemented, the salinity is controlled to be ≥5, the calcium magnesium mass ratio is 1:2-1:3, the total alkalinity is 120-150 mg / L, and the total hardness calculated as CaCO3 is 500-1000 mg / L.

9. The in-situ ecological treatment method according to claim 1, characterized in that: The results of the water quality verification include: ammonia nitrogen ≤ 0.6 mg / L, nitrite ≤ 0.2 mg / L.

10. Use of the in-situ ecological treatment method according to any one of claims 1 to 9 in treating shrimp aquaculture tail water.

Images