Safener for protecting dendrobium officinale from being damaged by herbicide and application of safener
By preparing taurine solution and Tween-20 as a safener, combined with specific light and temperature conditions, the problem of glyphosate damage to Dendrobium officinale was solved, the herbicide resistance of Dendrobium officinale was improved, the damage caused by the drug was reduced and the antioxidant capacity was enhanced.
Patent Information
- Application Number
- CN202411632473.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-15
- Publication Date
- 2025-09-09
AI Technical Summary
During the cultivation of Dendrobium officinale, the drift of the spray liquid of glyphosate herbicide causes phytotoxicity, destroys the ultrastructure of plant leaves, affects the efficiency of photosynthesis, and in severe cases leads to the death of Dendrobium officinale. There are few types of safeners available and their effects are poor.
A safener was prepared by mixing taurine solution with Tween-20 and sprayed on the leaves of Dendrobium officinale seedlings. Combined with specific light and temperature conditions, the herbicide resistance of Dendrobium officinale was improved.
Significantly reduced the damage of glyphosate to Dendrobium officinale seedlings, increased leaf chlorophyll content, reduced malondialdehyde and hydrogen peroxide content, enhanced peroxidase and ascorbic acid peroxidase activity, and reduced the degree of leaf yellowing.
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Abstract
Description
Technical Field
[0001] The present invention belongs to the technical field of herbicide safeners, and in particular relates to a safener for protecting Dendrobium officinale from the harm of herbicides and an application thereof. Background Art
[0002] Dendrobium officinale is a precious Chinese medicinal herb, considered one of China's "Nine Immortal Herbs." It boasts benefits such as stomach nourishment, immune regulation, and anti-aging. During its cultivation, especially when tissue culture seedlings are transferred to greenhouse culture media, herbicides are often used to effectively control weeds, ensure adequate nutrition for the plants, improve yield and quality, and reduce agricultural production costs associated with manual weeding. However, glyphosate-induced herbicide damage to Dendrobium officinale due to spray drift and other factors is common. For example, glyphosate damages plant leaf ultrastructures, such as chloroplasts and mitochondria, significantly reducing photosynthetic efficiency. Furthermore, glyphosate impairs the absorption and conversion of light energy, further inhibiting photosynthesis.
[0003] Safeners, also known as antidotes or protectants, are compounds used in herbicides to protect crops from herbicide damage, increasing crop safety and improving weed control. Since Hoffman first proposed the concept of safeners in 1962, nearly 20 varieties have been commercialized internationally. However, very few safeners are currently available for use in herbicides. The use of glyphosate can, at best, hinder the growth of Dendrobium officinale and, at worst, kill it, impacting farmers' incomes.
[0004] Research on Dendrobium officinale mostly focuses on tissue culture technology, cultivation methods, medicinal value, etc., and there is a lack of research and reports on the use of protective agents to improve Dendrobium officinale's tolerance to glyphosate. Summary of the Invention
[0005] In view of this, the object of the present invention is to provide a safener for protecting Dendrobium officinale from the harm of herbicides and its application, wherein the safener can significantly reduce the damage of organophosphorus herbicides to Dendrobium officinale seedlings and increase the herbicide resistance of Dendrobium officinale.
[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:
[0007] The invention provides a safener for protecting Dendrobium officinale from the harm of herbicides. The safener is prepared by mixing a taurine solution and Tween-20 in a volume ratio of 100:0.1-0.5. The concentration of the taurine solution is 200-300 mg / L.
[0008] The present invention also provides an application of the safener in improving the herbicide resistance of Dendrobium officinale.
[0009] Preferably, the herbicide comprises an organophosphorus herbicide.
[0010] Preferably, the organophosphorus herbicide is glyphosate.
[0011] The present invention also provides a method for improving the herbicide resistance of Dendrobium officinale, comprising the following steps: spraying the safener on the leaves of Dendrobium officinale seedlings grown to 6 to 9 months old, spraying once every 1 to 2 days.
[0012] Preferably, each spray should be applied until the leaves are completely wet and drips begin to appear at the leaf tips.
[0013] Preferably, the Dendrobium officinale seedlings sprayed with the safener are cultured at 22-25° C. with a photoperiod of 12 hours light / 12 hours dark.
[0014] Preferably, the light intensity of the culture is 3000-5000 lux.
[0015] Preferably, the herbicide comprises an organophosphorus herbicide.
[0016] More preferably, the organophosphorus herbicide includes glyphosate.
[0017] Compared with the prior art, the present invention has the following beneficial effects:
[0018] The present invention provides a safener for protecting Dendrobium officinale from herbicide damage. The safener is prepared from a taurine solution and Tween-20. After being used to treat Dendrobium officinale seedlings, the results showed that pretreatment with the safener for 7 days significantly reduced the damage of glyphosate to the Dendrobium officinale seedlings. Compared with a control group, the leaves of the Dendrobium officinale seedlings pretreated with the safener showed significantly reduced yellowing, higher chlorophyll content, lower malondialdehyde (MDA) and hydrogen peroxide (H2O2) contents, and significantly higher peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) activities than the control group. The present invention provides a reference basis for the large-scale promotion and application and planting and cultivation of Dendrobium officinale. BRIEF DESCRIPTION OF THE DRAWINGS
[0019] Figure 1 Figure 2 shows the growth status of Dendrobium officinale seedlings that were pretreated with the safener of the present invention or water for 7 days and then sprayed with glyphosate or grown with water for 7 days. In the figure, H2OH2O shows seedlings that were pretreated with water for 7 days and then sprayed with water for 7 days; H2OGly shows seedlings that were pretreated with water for 7 days and then sprayed with glyphosate for 7 days; TauH2O shows seedlings that were pretreated with taurine for 7 days and then sprayed with water for 7 days; and TauGly shows seedlings that were pretreated with taurine for 7 days and then sprayed with glyphosate for 7 days. DETAILED DESCRIPTION
[0020] The present invention provides a safener for protecting Dendrobium officinale from the harm of herbicides. The safener is preferably prepared by mixing a taurine solution and Tween-20 in a volume ratio of 100:0.1 to 0.5, that is, 0.1 to 0.5 mL of Tween-20 is contained in every 100 mL of taurine solution. In the present invention, the concentration of the taurine solution is preferably 200 to 300 mg / L. In the present invention, sterile water is preferably used to prepare a taurine solution with a concentration of 200 to 300 mg / L. The present invention does not specifically limit the sources of the taurine and Tween-20 raw materials, and the raw materials can be purchased through conventional channels.
[0021] The present invention also provides a use of the safener for improving herbicide resistance in Dendrobium officinale. In the present invention, the herbicide preferably comprises an organophosphorus herbicide; the organophosphorus herbicide is preferably glyphosate. In the present invention, the safener is more preferably used to improve herbicide resistance in tissue cultured Dendrobium officinale.
[0022] The present invention also provides a method for improving the herbicide resistance of Dendrobium officinale, comprising the following steps: spraying the safener on the leaves of Dendrobium officinale seedlings grown to 6 to 9 months old, spraying once every 1 to 2 days. In the present invention, the Dendrobium officinale seedlings are preferably obtained by tissue culture. In the present invention, the growth medium for obtaining Dendrobium officinale seedlings by tissue culture preferably includes the following raw materials: 1 / 2MS medium, 20g / L banana puree, 20g / L sucrose, and 0.1g / L activated carbon. In the present invention, more preferably, the safener is sprayed on the leaves of Dendrobium officinale seedlings grown to 8 months old. The spraying is based on the standard of each spraying until the leaves are completely wet and dripping begins to appear at the leaf tips.
[0023] In the present invention, the Dendrobium officinale seedlings sprayed with the safener are preferably cultured at 22-25° C. with a photoperiod of 12 hours light / 12 hours dark; the light intensity of the culture is preferably 3000-5000 lux.
[0024] In the present invention, the herbicide preferably includes an organophosphorus herbicide; the organophosphorus herbicide preferably includes glyphosate.
[0025] The technical solutions provided by the present invention are described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.
[0026] The experimental methods in the following examples are conventional methods unless otherwise specified. The experimental materials used in the following examples are commercially available products unless otherwise specified.
[0027] Example 1
[0028] A safener for protecting Dendrobium officinale from the harm of herbicides. The safener is prepared by mixing a taurine solution and Tween-20 in a volume ratio of 100:0.1. The concentration of the taurine solution is 300 mg / L.
[0029] Example 2
[0030] A safener for protecting Dendrobium officinale from the harm of herbicides. The safener is prepared by mixing a taurine solution and Tween-20 in a volume ratio of 100:0.5. The concentration of the taurine solution is 300 mg / L.
[0031] Example 3
[0032] A safener for protecting Dendrobium officinale from the harm of herbicides. The safener is prepared by mixing a taurine solution and Tween-20 in a volume ratio of 100:0.3. The concentration of the taurine solution is 200 mg / L.
[0033] Example 4
[0034] A safener for protecting Dendrobium officinale from the harm of herbicides. The safener is prepared by mixing a taurine solution and Tween-20 in a volume ratio of 100:0.3. The concentration of the taurine solution is 250 mg / L.
[0035] Example 5
[0036] A method for improving the herbicide resistance of Dendrobium officinale comprises the following steps:
[0037] Wild Dendrobium officinale seeds were selected and tissue cultured to obtain well-grown 8-month-old Dendrobium officinale seedlings. The growth medium was 1 / 2 MS medium with 20 g / L banana puree, 20 g / L sucrose, and 0.1 g / L activated carbon.
[0038] Select healthy Dendrobium officinale seedlings with uniform growth, and spray them with the safener obtained in Example 1. Apply it in the form of foliar spraying until the leaves are completely wet and dripping downwards. Spray once every two days for a total of two times;
[0039] Afterwards, the cells were placed in an artificial climate box for normal growth for 7 days. The conditions of the artificial climate box were set as follows: temperature 25°C; photoperiod 12 hours / 12 hours, and light intensity 3000-5000 lux.
[0040] Example 6
[0041] The difference from Example 5 is that the safener obtained in Example 2 is sprayed, and the other steps remain unchanged.
[0042] Example 7
[0043] The difference from Example 5 is that the safener obtained in Example 3 is sprayed, and the other steps remain unchanged.
[0044] Example 8
[0045] The difference from Example 5 is that the safener obtained in Example 4 is sprayed, and the other steps remain unchanged.
[0046] Comparative Example 1
[0047] The difference from Example 5 is that clean water is sprayed, and the other steps remain unchanged.
[0048] Example 9
[0049] This example verifies the application of the safener of Example 1 in improving the herbicide resistance of Dendrobium officinale. The specific test steps are as follows:
[0050] 1. Test method
[0051] (1) Wild Dendrobium officinale seeds were selected and tissue cultured to obtain 8-month-old well-grown seedlings, with approximately 20 seedlings per bottle. The growth medium was 1 / 2 MS medium with 20 g / L banana puree, 20 g / L sucrose, and 0.1 g / L activated carbon.
[0052] (2) Select healthy and uniformly growing Dendrobium officinale seedlings for the experiment. Spray them with a 300 mg / L taurine solution containing 0.1% Tween-20 prepared with sterile water in a sterile operating table. Apply the solution to the leaves by spraying until the leaves are completely wet and dripping. Spray once every two days for a total of two times.
[0053] (3) Afterwards, the plants were placed in an artificial climate chamber for normal growth for 7 days. The conditions of the artificial climate chamber were set as follows: temperature 25°C; photoperiod 12 hours / 12 hours, and light intensity 3000-5000 lux;
[0054] (4) After 7 days of pretreatment, the Dendrobium officinale seedlings were sprayed with glyphosate solution for stress treatment at a concentration of 5 g / L; the spraying was carried out until the leaves were completely wet and dripping began to appear at the leaf tips;
[0055] (5) The growth status of D. officinale seedlings was observed on day 0, day 1, day 4, and day 7 of glyphosate treatment. Leaves of D. officinale seedlings were collected, quickly frozen in liquid nitrogen, and stored at -80°C for key parameter determination. To reduce experimental error, three replicates were set for all treatments.
[0056] Setting up a control group: The difference from the above test method is that water is sprayed in step (2), and the remaining steps are the same as the above test method.
[0057] 2. Measurement indicators
[0058] (1) Phenotypic observation: The growth of D. officinale seedlings treated with glyphosate or water (control) for 7 days was observed and photographed.
[0059] (2) Determination of photosynthetic pigment content: The determination method refers to the chlorophyll and carotenoid content determination method in "Principles and Techniques of Plant Physiology and Biochemistry Experiments".
[0060] (3) Determination of malondialdehyde (MDA) content: Weigh 0.1 g of sample and place in a pre-chilled mortar. Add 1 mL of pre-chilled phosphate buffer (pH 7.5) and grind into a slurry. Centrifuge at 10,000 g for 10 min at 4°C. Remove the supernatant and use it for analysis. MDA content was determined using an MDA spectrophotometer kit (provided by Beijing Solebold Technology Co., Ltd., catalog number BC0020).
[0061] (4) Determination of hydrogen peroxide (H2O2) content: Samples were extracted with acetone for subsequent determination using a H2O2 content detection kit provided by Beijing Solebow Technology Co., Ltd., catalog number BC3590.
[0062] (5) Determination of peroxidase (POD) activity: The sample extraction method was the same as the MDA extraction method. The activity was determined using a POD activity detection kit provided by Beijing Solebow Technology Co., Ltd., catalog number BC0090.
[0063] (6) Determination of catalase (CAT) activity: The sample extraction method was the same as the MDA extraction method. CAT activity was determined using a CAT activity detection kit provided by Beijing Solebow Technology Co., Ltd., catalog number BC4780.
[0064] (7) Determination of ascorbate peroxidase (APX) activity: The sample extraction method was the same as the MDA extraction method. APX activity was determined using the APX activity detection kit, provided by Beijing Solebow Technology Co., Ltd., catalog number BC0220.
[0065] 3. Results Analysis
[0066] (1) According to Figure 1 The results show that there is a significant difference in the degree of leaf yellowing after glyphosate spraying between Dendrobium officinale seedlings pretreated with the safener of the present invention and those pretreated with water. Pretreatment with the safener of the present invention significantly reduces glyphosate damage to Dendrobium officinale seedlings, with the degree of leaf yellowing being lower than that of seedlings pretreated with water.
[0067] (2) According to the results in Table 1, the safener pretreatment of the present invention can effectively reduce the degradation rate of photosynthetic pigments in the leaves of Dendrobium officinale under glyphosate stress. As the glyphosate treatment time increases, the chlorophyll a content of the Dendrobium officinale seedlings pretreated with water gradually decreases, from 0.26±0.06 at H2OGly-0d to 0.13±0.02 at H2OGly-7d. The degradation rate of the chlorophyll a content of the Dendrobium officinale seedlings pretreated with the safener of the present invention slows down, from 0.22±0.01 at TauGly-0d to 0.15±0.01 at TauGly-7d. After 7 days of glyphosate treatment, the chlorophyll a content in the leaves of the seedlings pretreated with the safener of the present invention is higher than that of the seedlings pretreated with water.
[0068] As glyphosate treatment time increased, the chlorophyll b content of water-pretreated Dendrobium officinale seedlings gradually decreased, from 0.14±0.01 at H2OGly-0d to 0.04±0.01 at H2OGly-7d. Chlorophyll b degradation in Dendrobium officinale seedlings pretreated with the safener of the present invention slowed, decreasing from 0.08±0.01 at TauGly-0d to 0.05±0.01 at TauGly-7d. After seven days of glyphosate treatment, the chlorophyll b content in the leaves of seedlings pretreated with the safener of the present invention was higher than that of seedlings pretreated with water.
[0069] As glyphosate treatment time increased, the carotenoid content in water-pretreated Dendrobium officinale seedlings gradually decreased, from 0.06±0.00 at H2OGly-0 day to 0.04±0.01 at H2OGly-7 day. The degradation rate of carotenoid content in Dendrobium officinale seedlings pretreated with the safener of the present invention slowed, decreasing from 0.08±0.01 at TauGly-0 day to 0.05±0.01 at TauGly-7 day. After seven days of glyphosate treatment, the carotenoid content in the leaves of seedlings pretreated with the safener of the present invention was higher than that of seedlings pretreated with water.
[0070] Table 1 Chlorophyll and carotenoid contents in leaves of Dendrobium officinale seedlings after different treatments
[0071] Sample name Chlorophyll a content mg / g Chlorophyll b content mg / g Carotenoid content mg / g <![CDATA[H2OGly-0d]]> 0.26±0.06ab 0.14±0.01a** 0.06±0.00a* <![CDATA[H2OGly-1d]]> 0.24±0.02a 0.08±0.02b 0.07±0.02a <![CDATA[H2OGly-4d]]> 0.15±0.02bc* 0.03±0.01c* 0.06±0.02a <![CDATA[H2OGly-7d]]> 0.13±0.02c** 0.04±0.01c* 0.04±0.01b TauGly-0d 0.22±0.01a 0.08±0.01a 0.08±0.00a TauGly-1d 0.22±0.01a 0.07±0.00a 0.07±0.00a TauGly-4d 0.20±0.03a 0.07±0.01a 0.06±0.01a TauGly-7d 0.15±0.01b 0.05±0.01b 0.05±0.00b
[0072] Note: In the table, H2OGly-0d is the seedling that was grown for 0 days after 7 days of water pretreatment and spraying with glyphosate; H2OGly-1d is the seedling that was grown for 1 day after 7 days of water pretreatment and spraying with glyphosate; H2OGly-4d is the seedling that was grown for 4 days after 7 days of water pretreatment and spraying with glyphosate; H2OGly-7d is the seedling that was grown for 7 days after 7 days of water pretreatment and spraying with glyphosate; TauGly-0d is the seedling that was grown for 0 days after 7 days of water pretreatment with the safener of the present invention and spraying with glyphosate; TauGly-1d is the seedling that was grown for 7 days after 7 days of water pretreatment with the safener of the present invention and spraying with glyphosate. Seedlings grown 1 day after glyphosate spraying; TauGly-4d refers to seedlings grown 4 days after being pretreated with the safener of the present invention for 7 days and then sprayed with glyphosate; TauGly-7d refers to seedlings grown 7 days after being pretreated with the safener of the present invention for 7 days and then sprayed with glyphosate; different lowercase letters in the table indicate significant differences (p<0.05) between the safener of the present invention or water pretreatment for 7 days and the glyphosate spraying for different days; *(p<0.05) and **(p<0.01) indicate significant differences between the safener of the present invention sprayed for the same number of days after being pretreated with water for 7 days and the safener of the present invention for 7 days.
[0073] (3) According to the results in Table 2, taurine pretreatment can reduce the MDA content in the leaves of Dendrobium officinale. As the glyphosate treatment time increases, the MDA content of the water-pretreated Dendrobium officinale seedlings gradually increases from 4.37±0.63 at H2OGly-0d to 7.66±1.26 at H2OGly-4d, and then decreases. Although the MDA content of the taurine-pretreated Dendrobium officinale seedlings also increases after spraying glyphosate, the maximum value is only 3.73±0.72 at TauGly-1d, which is significantly lower than that of the water-pretreated Dendrobium officinale seedlings.
[0074] As glyphosate treatment time increased, the H2O2 content in water-pretreated D. officinale seedlings gradually increased, from 0.21±0.02 at H2OGly-0d to 0.61±0.06 at H2OGly-7d. However, the H2O2 content in taurine-pretreated D. officinale seedlings, while showing a slow increase after glyphosate spraying, increased from 0.27±0.02 at TauGly-0d to 0.35±0.01 at TauGly-7d, was significantly lower than the 0.61±0.06 at H2OGly-7d in water-pretreated D. officinale seedlings.
[0075] Table 2 MDA and H2O2 contents in leaves of Dendrobium officinale seedlings under different treatments
[0076] Sample name MDA content nmol / mgprot <![CDATA[H2O2 content mmol / mgprot]]> <![CDATA[H2OGly-0d]]> 4.37±0.63a 0.21±0.02a* <![CDATA[H2OGly-1d]]> 5.13±0.58a 0.25±0.08ab <![CDATA[H2OGly-4d]]> 7.66±1.26b** 0.28±0.02b <![CDATA[H2OGly-7d]]> 3.97±1.50a** 0.61±0.06c** TauGly-0d 1.91±0.09a 0.27±0.02a TauGly-1d 3.73±0.72b 0.35±0.04b TauGly-4d 2.31±0.69ab 0.34±0.03b TauGly-7d 2.03±0.39a 0.35±0.01b
[0077] Note: In the table, H2OGly-0d is the seedling that was grown for 0 days after 7 days of water pretreatment and spraying with glyphosate; H2OGly-1d is the seedling that was grown for 1 day after 7 days of water pretreatment and spraying with glyphosate; H2OGly-4d is the seedling that was grown for 4 days after 7 days of water pretreatment and spraying with glyphosate; H2OGly-7d is the seedling that was grown for 7 days after 7 days of water pretreatment and spraying with glyphosate; TauGly-0d is the seedling that was grown for 0 days after 7 days of water pretreatment with the safener of the present invention and spraying with glyphosate; TauGly-1d is the seedling that was grown for 7 days after 7 days of water pretreatment with the safener of the present invention and spraying with glyphosate. Seedlings grown 1 day after glyphosate spraying; TauGly-4d refers to seedlings grown 4 days after being pretreated with the safener of the present invention for 7 days and then sprayed with glyphosate; TauGly-7d refers to seedlings grown 7 days after being pretreated with the safener of the present invention for 7 days and then sprayed with glyphosate; different lowercase letters in the table indicate significant differences (p<0.05) between the safener of the present invention or water pretreatment for 7 days and the glyphosate spraying for different days; *(p<0.05) and **(p<0.01) indicate significant differences between the safener of the present invention sprayed for the same number of days after being pretreated with water for 7 days and the safener of the present invention for 7 days.
[0078] (4) According to the results in Table 3, the safener pretreatment of the present invention can reduce the POD activity in the leaves of Dendrobium officinale. As the glyphosate treatment time increases, the POD activity of the Dendrobium officinale seedlings pretreated with water gradually increases, from 9.63±0.83 at H2OGly-0d to 21.71±2.11 at H2OGly-7d. The POD activity of the Dendrobium officinale seedlings pretreated with the safener of the present invention showed a more significant increase after spraying glyphosate, from 10.04±0.16 at TauGly-0d to 26.92±1.15 at TauGly-7d. At 0 and 7 days after glyphosate treatment, the POD activity in the leaves of the seedlings pretreated with the safener of the present invention was higher than that of the seedlings pretreated with water.
[0079] As glyphosate treatment time increased, CAT activity in water-pretreated Dendrobium officinale seedlings gradually increased, from 71.64±6.36 at H2OGly-0 day to 76.29±8.25 at H2OGly-7 day. CAT activity in Dendrobium officinale seedlings pretreated with the safener of the present invention showed an even more significant increase after glyphosate application, from 58.49±6.63 at TauGly-0 day to 126.26±10.29 at TauGly-7 day. Even after seven days of glyphosate treatment, CAT activity in the leaves of seedlings pretreated with the safener of the present invention was higher than that in seedlings pretreated with water.
[0080] With the extension of glyphosate treatment time, the APX activity of Dendrobium officinale seedlings pretreated with water gradually increased, from 0.02±0.01 at H2OGly-0 day to 0.04±0.01 at H2OGly-7 day. The APX activity of Dendrobium officinale seedlings pretreated with the safener of the present invention showed an even more significant increase after glyphosate spraying, from 0.04±0.01 at TauGly-0 day to 0.11±0.01 at TauGly-7 day. At both 0 and 7 days after glyphosate treatment, the APX activity in the leaves of seedlings pretreated with the safener of the present invention was higher than that in seedlings pretreated with water.
[0081] Table 3 Antioxidant enzyme activities in leaves of Dendrobium officinale seedlings under different treatments
[0082]
[0083] Note: In the table, H2OGly-0d is the seedling that was grown for 0 days after 7 days of water pretreatment and spraying with glyphosate; H2OGly-1d is the seedling that was grown for 1 day after 7 days of water pretreatment and spraying with glyphosate; H2OGly-4d is the seedling that was grown for 4 days after 7 days of water pretreatment and spraying with glyphosate; H2OGly-7d is the seedling that was grown for 7 days after 7 days of water pretreatment and spraying with glyphosate; TauGly-0d is the seedling that was grown for 0 days after 7 days of water pretreatment with the safener of the present invention and spraying with glyphosate; TauGly-1d is the seedling that was grown for 7 days after 7 days of water pretreatment with the safener of the present invention and spraying with glyphosate. Seedlings grown 1 day after glyphosate spraying; TauGly-4d refers to seedlings grown 4 days after being pretreated with the safener of the present invention for 7 days and then sprayed with glyphosate; TauGly-7d refers to seedlings grown 7 days after being pretreated with the safener of the present invention for 7 days and then sprayed with glyphosate; different lowercase letters in the table indicate significant differences (p<0.05) between the safener of the present invention or water pretreatment for 7 days and the glyphosate spraying for different days; *(p<0.05) and **(p<0.01) indicate significant differences between the safener of the present invention sprayed for the same number of days after being pretreated with water for 7 days and the safener of the present invention for 7 days.
[0084] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.
Claims
1. A safener for protecting Dendrobium officinale from herbicide hazards, characterized in that: The safety agent is prepared by mixing taurine solution and Tween-20 in a volume ratio of 100:0.1-0.5, and the concentration of the taurine solution is 200-300 mg / L.
2. Use of the safener according to claim 1 in improving the herbicide resistance of Dendrobium officinale.
3. The use according to claim 2, characterized in that The herbicides include organophosphorus herbicides.
4. The use according to claim 3, characterized in that The organophosphorus herbicide is glyphosate.
5. A method for improving the herbicide resistance of Dendrobium officinale, characterized in that: The method comprises the following steps: spraying the safener according to claim 1 onto the leaves of Dendrobium officinale seedlings that are 6 to 9 months old, and spraying once every 1 to 2 days.
6. The method according to claim 5, characterized in that Spray each time until the leaves are completely wet and drips begin to appear on the leaf tips.
7. The method according to claim 5, characterized in that The Dendrobium officinale seedlings sprayed with the safener were cultured at 22-25°C with a photoperiod of 12 hours light / 12 hours dark.
8. The method according to claim 7, characterized in that The light intensity of the culture is 3000-5000 lux.
9. The method according to claim 5, characterized in that The herbicides include organophosphorus herbicides.
10. The method according to claim 9, characterized in that The organophosphorus herbicide includes glyphosate.