Bird's nest acid composition with whitening, brightening and anti-saccharification functions as well as preparation method and cosmetics thereof

Abstract

The invention relates to the field of cosmetics, in particular to a sialic acid composition with whitening, brightening and anti-saccharification functions, a preparation method of the sialic acid composition and cosmetics. The sialic acid composition is prepared from the following components in parts by mass: 4 to 10 parts of sialic acid, 8 to 15 parts of carnosine and 15 to 25 parts of nicotinamide. According to the sialic acid composition with the whitening, brightening and anti-saccharification functions, sialic acid, nicotinamide and carnosine are matched and synergistically exert effects, and the sialic acid composition can enhance the epidermal barrier function, promote permeation of active ingredients, remove AGEs, block generation of saccharification end products, inhibit melanin transport and regulate cell metabolism, so that the skin whitening, brightening and anti-saccharification functions are achieved. Finally, the three components form a synergistic pathway of blocking saccharification, inhibiting melanin and transdermal permeation promotion; therefore, the problems of uneven skin color and darkness of the skin are fundamentally solved.

Description

Technical Field

[0001] The present application relates to the field of cosmetics, and more specifically, to a bird's nest acid composition having whitening, brightening and anti-glycation functions, a preparation method thereof, and cosmetics. Background Art

[0002] As we age, our skin becomes more prone to uneven skin tone and dullness. The current conventional solution is to use whitening skin care products to whiten the skin.

[0003] Common whitening skincare products on the market today contain traditional whitening ingredients such as vitamin C derivatives, niacinamide, arbutin, tranexamic acid, and kojic acid. However, these traditional whitening ingredients are highly irritating, requiring initial tolerance to build up. Otherwise, they can cause skin discomfort, such as redness and stinging. Other ingredients only target a specific part of melanin production, failing to fully address uneven skin tone and dullness. Their whitening effects are limited and prone to recurrence. Summary of the Invention

[0004] The purpose of the embodiments of the present application is to provide a bird's nest acid composition with whitening, brightening and anti-glycation functions, a preparation method thereof, and cosmetics.

[0005] In the first aspect, the present application provides a bird's nest acid composition with whitening, brightening and anti-glycation functions. Calculated by weight, the bird's nest acid composition includes: 4-10 parts of bird's nest acid, 8-15 parts of carnosine and 15-25 parts of niacinamide.

[0006] In the above technical solution, the bird's nest acid composition with whitening, brightening and anti-glycation functions is composed of bird's nest acid, niacinamide and carnosine to synergistically exert its efficacy. The bird's nest acid composition can enhance the epidermal barrier function, promote the penetration of active ingredients, remove AGEs, block the formation of glycation end products, inhibit melanin transport, and regulate cell metabolism. Ultimately, the three form a synergistic pathway of "blocking glycation-inhibiting melanin-transdermal penetration promotion", thereby fundamentally solving the problem of uneven skin tone and dullness. Furthermore, the whitening and brightening anti-glycation bird's nest acid composition is compounded with bird's nest acid, carnosine and niacinamide within the above-mentioned ratio range, which can achieve different ways of inhibiting human tyrosinase activity from the root to reduce melanin synthesis, blocking the transport of melanosomes from melanocytes to surrounding keratinocytes in the process, scavenging free radicals, inhibiting the formation of glycation end products AGEs, protecting cells, soothing the skin, etc., to form a synergistic whitening effect of scavenging free radicals, resisting glycation, reducing glycated proteins, inhibiting tyrosinase activity, and reducing melanin production and transport. It is not only more active, but also less irritating, more mild and safer. In actual use, it has the effect of brightening skin tone, increasing skin gloss and even skin tone.

[0007] In other embodiments of the present application, the above-mentioned bird's nest acid composition includes, by weight, 4-8 parts of bird's nest acid, 8-12 parts of carnosine, and 15-24 parts of niacinamide.

[0008] In other embodiments of the present application, the bird's nest acid composition includes, by weight: 4-6 parts of bird's nest acid, 8-12 parts of carnosine, and 15-24 parts of niacinamide.

[0009] In other embodiments of the present application, in the bird's nest acid composition, the mass ratio of bird's nest acid to carnosine is 0.27~1.25.

[0010] In other embodiments of the present application, in the bird's nest acid composition, the mass ratio of bird's nest acid to niacinamide is 0.16~0.67.

[0011] In other embodiments of the present application, in the bird's nest acid composition, the mass ratio of carnosine to niacinamide is 0.32~1.00.

[0012] In other embodiments of the present application, the bird's nest acid composition having whitening, brightening and anti-glycation functions includes: a pH regulator.

[0013] In other embodiments of the present application, the amount of the pH regulator in the bird's nest acid composition is 1 to 5 parts by weight.

[0014] In other embodiments of the present application, the pH regulator includes: at least one of an acidic regulator or an alkaline regulator; optionally, the pH regulator includes at least one of arginine solution, citric acid solution, triethanolamine, sodium hydroxide solution or tromethamine.

[0015] In other embodiments of the present application, the bird's nest acid composition with whitening, brightening and anti-glycation functions further comprises: Antioxidant and preservative.

[0016] In other embodiments of the present application, in the bird's nest acid composition, the amount of antioxidant is 0.1-5 parts; the amount of preservative is 0.5-10 parts; Optionally, the antioxidant includes: at least one of sodium bisulfite, sodium sulfite or sodium metabisulfite; Optionally, the preservative includes: a water-soluble preservative; optionally, the preservative includes: at least one of phenoxyethanol, ethylhexylglycerin, 1,2-pentanediol, 1,3-butanediol, 1,2-hexanediol or p-hydroxyacetophenone.

[0017] In a second aspect, the present application provides a method for preparing a bird's nest acid composition having whitening, brightening and anti-glycation functions, comprising: Pre-dissolving senna and carnosine in water to obtain a first mixed solution; adjusting the pH of the first mixed solution to 5.5-6.5 using a pH regulator to obtain a second mixed solution; adding nicotinamide to the second mixed solution and dissolving and mixing; Optionally, the pre-dissolving temperature is below 45°C; Optionally, after adding nicotinamide and dissolving and mixing, a pH adjuster is added again to adjust the pH of the mixture to 5.5-6.5.

[0018] In a third aspect, the present application provides a cosmetic comprising: a bird's nest acid composition having whitening, brightening and anti-glycation functions provided by any of the aforementioned embodiments; Optionally, in the cosmetic, the amount of the bird's nest acid composition with whitening, brightening and anti-glycation functions added is 0.1%-20% (w / w) in terms of volume percentage. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following is a brief introduction to the drawings required for use in the embodiments. It should be understood that the following drawings only show certain embodiments of the present application and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without creative work.

[0020] Figure 1 Schematic diagram of the essence.

[0021] Figure 2 This is an example diagram of the human efficacy test of the essence prepared in Application Example 1. DETAILED DESCRIPTION

[0022] In order to make the purpose, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments.

[0023] Therefore, the following detailed description of the embodiments of the present application is not intended to limit the scope of the present application for protection, but merely represents selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of this application.

[0024] The inventors discovered that uneven skin tone and dullness are primarily caused by two factors: First, exposure to ultraviolet radiation and environmental pollution stimulates increased melanin production in the basal layer of the epidermis, leading to dull skin and the formation of dark spots. Second, glycation reactions in the skin intensify with aging and the effects of factors like a high-sugar diet and poor sleep habits in modern life. Excessive accumulation of advanced glycation end products (AGEs) not only destroys collagen and elastic fibers in the skin, causing sagging and increased wrinkles, but also leads to a yellowish, dull, and uneven complexion. Therefore, traditional whitening ingredients alone are insufficient to completely address these issues. A synergistic approach to anti-glycation and even skin tone is needed to fundamentally address these issues.

[0025] Further research has revealed that skin glycation refers to the process by which sugar and protein in the body combine, leading to protein denaturation. When we consume too much sugar, blood sugar levels rise. Excessive sugar reacts with collagen in the skin, forming substances called advanced glycation end products (AGEs). These AGEs denature the protein, causing it to lose its original structure and function. The formation of AGEs leads to protein cross-linking, making collagen fibers rigid and brittle. This can lead to a decrease in skin elasticity and firmness, resulting in sagging, wrinkles, and dullness. AGEs can also accumulate in the deeper layers of the skin, causing hyperpigmentation and dark spots. Whitening products can inhibit melanin production at the root, while anti-glycation products can effectively block the damaging effects of glycation. The combined effect of these two methods is crucial for achieving overall brighter and more even skin tone.

[0026] Based on this, the embodiment of the present application provides a bird's nest acid composition with whitening, brightening and anti-glycation functions. Calculated by weight, the bird's nest acid composition includes: 4-10 parts of bird's nest acid, 8-15 parts of carnosine and 15-25 parts of niacinamide.

[0027] In the above technical solution, the bird's nest acid composition with whitening, brightening and anti-glycation functions is composed of bird's nest acid, niacinamide and carnosine to synergistically exert its efficacy. The bird's nest acid composition can enhance the epidermal barrier function, promote the penetration of active ingredients, remove AGEs, block the formation of glycation end products, inhibit melanin transport, and regulate cell metabolism. Ultimately, the three form a synergistic pathway of "blocking glycation-inhibiting melanin-transdermal penetration promotion", thereby fundamentally solving the problem of uneven skin tone and dullness.

[0028] Illustratively, in some embodiments of the present application, the above-mentioned bird's nest acid composition includes, by weight: 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts of bird's nest acid or a range between any two of the foregoing values; 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts of carnosine or a range between any two of the foregoing values; and 15 parts, 16 parts, 17 parts, 18 parts, 19 parts, 20 parts, 21 parts, 22 parts, 23 parts, 24 parts, 25 parts of niacinamide or a range between any two of the foregoing values.

[0029] Bird's nest acid (N-acetylneuraminic acid), also known as sialic acid, is a naturally occurring amino sugar and an important quantitative indicator for evaluating the quality grade of bird's nest. As an endogenous anti-aging active ingredient, bird's nest acid is widely present in cell membranes, primarily in the form of oligosaccharides, glycolipids, and glycoproteins. Literature and third-party efficacy testing have demonstrated that bird's nest acid, when applied topically to skin care, exhibits multiple benefits, including anti-aging, antioxidant, anti-glycation, anti-wrinkle, firming, and skin protection. Internal use can modulate immune function, promote intestinal health, and possess anti-aging and antioxidant properties. Bird's nest acid truly embodies the "same source for beauty and food," making it a high-value cosmetic ingredient with both skincare and health benefits. However, further research is needed to fully utilize bird's nest acid in bird's nest compositions for whitening, brightening, and anti-glycation. In the above technical solution, by compounding bird's nest acid with carnosine and niacinamide, a synergistic effect can be achieved, further enhancing the effect of the entire bird's nest acid composition in inhibiting melanin, enhancing transdermal penetration, synergistically promoting penetration, and blocking glycation, thereby further enhancing the whitening, brightening and anti-glycation effects of the entire bird's nest acid composition.

[0030] Nicotinamide, also known as nicotinamide or vitamin B3, is an important derivative of niacin and is widely distributed in various foods. When used in cosmetics, nicotinamide can act on the already produced melanin and effectively inhibit the transport of melanosomes by approximately 35% to 68% in the co-culture model. In the above technical solution, by compounding nicotinamide with bird's nest acid and carnosine, a synergistic effect can be achieved, enhancing the effects of the entire bird's nest acid composition in inhibiting melanin, enhancing transdermal penetration, blocking glycation, and synergistically promoting penetration, thereby further enhancing the whitening, brightening and anti-glycation effects of the entire bird's nest acid composition.

[0031] Carnosine, scientifically known as β-alanyl-L-histidine, is a dipeptide composed of the amino acids β-alanine and L-histidine. It is found in high concentrations in the brain and skeletal muscle tissue of vertebrates. Carnosine possesses strong antioxidant activity, acting as a hydrogen acceptor to scavenge reactive oxygen species (ROS) and α-β-unsaturated aldehydes formed during the body's oxidative processes due to the overoxidation of fatty acids in cell membranes. Furthermore, carnosine can occupy sites in proteins where sugars and aldehydes normally bind, preventing the sugars from interacting with the protein and protecting it from glycation, thereby achieving an anti-glycation effect. Finally, carnosine can reduce native proteins through transglycosylation and degrade reactive carbonyl species (RCS), precursors of AGEs, thereby inhibiting the formation of advanced glycation end products (AGEs). In the above technical solution, by compounding niacinamide with bird's nest acid and carnosine, a synergistic effect can be achieved, thereby enhancing the blocking glycation, inhibiting melanin, enhancing transdermal penetration, and synergistic penetration of the entire bird's nest acid composition, thereby further enhancing the whitening, brightening and anti-glycation effects of the entire bird's nest acid composition.

[0032] In the above technical solution, the whitening and brightening anti-glycation bird's nest acid composition, bird's nest acid, carnosine and niacinamide are compounded within the above range, which can respectively inhibit the activity of human tyrosinase from the source to reduce melanin synthesis, block the transport of melanosomes from melanocytes to surrounding keratinocytes in the process, scavenge free radicals, inhibit the formation of glycation end products AGEs, protect cells, soothe the skin and other different ways to form a synergistic whitening effect of scavenging free radicals, anti-glycation, reducing glycated proteins, inhibiting tyrosinase activity, and reducing melanin production and transport. It is not only more active, but also less irritating, more mild and safer. In actual use, it has the effect of brightening skin tone, increasing skin gloss and even skin tone.

[0033] Further optionally, in some embodiments of the present application, the above-mentioned sialic acid (N-acetylneuraminic acid) has a CAS number of 131-48-6 and is required to have a purity of not less than 98%.

[0034] Further optionally, in some embodiments of the present application, the above-mentioned niacinamide, serial number 07359 in China's "Catalogue of Used Cosmetic Raw Materials (2021 Edition)", is purchased from Shanghai Baihaobo Biotechnology Co., Ltd.

[0035] Further optionally, in some embodiments of the present application, the above-mentioned carnosine has a serial number of 03150 and a CAS number of 305-84-0 in China's "Catalogue of Used Cosmetic Raw Materials (2021 Edition)", and the purity is required to be no less than 98%.

[0036] Further optionally, in some embodiments of the present application, the bird's nest acid composition includes, by weight: 4-8 parts of bird's nest acid, 8-12 parts of carnosine, and 15-24 parts of niacinamide.

[0037] Further optionally, in some embodiments of the present application, the bird's nest acid composition includes, by weight: 4-6 parts of bird's nest acid, 8-12 parts of carnosine, and 15-24 parts of niacinamide.

[0038] Further optionally, in some embodiments of the present application, in the bird's nest acid composition, the mass ratio of bird's nest acid to carnosine is 0.27~1.25.

[0039] Illustratively, in some embodiments of the present application, in the above-mentioned bird's nest acid composition, the mass ratio of bird's nest acid to carnosine is 0.27, 0.28, 0.29, 0.30, 0.31, 0.32, 0.35, 0.40, 0.50, 0.60, 0.70, 0.80, 1.00, 1.10, 1.15, 1.20, 1.25 or a range between any two of the foregoing values.

[0040] Furthermore, in some embodiments of the present application, in the bird's nest acid composition, the mass ratio of bird's nest acid to niacinamide is 0.16~0.67.

[0041] Illustratively, in some embodiments of the present application, in the above-mentioned bird's nest acid composition, the mass ratio of bird's nest acid to niacinamide is 0.16, 0.18, 0.20, 0.25, 0.30, 0.35, 0.40, 0.45, 0.50, 0.55, 0.60, 0.65, 0.67 or a range between any two of the foregoing values.

[0042] Furthermore, in some embodiments of the present application, in the bird's nest acid composition, the mass ratio of carnosine to niacinamide is 0.32~1.00.

[0043] Illustratively, in some embodiments of the present application, in the above-mentioned bird's nest acid composition, the mass ratio of carnosine to niacinamide is 0.32, 0.35, 0.40, 0.45, 0.50, 0.55, 0.60, 0.65, 0.70, 0.75, 0.80, 0.90, 0.95, 1.00 or a range between any two of the foregoing values.

[0044] Furthermore, in some embodiments of the present application, bird's nest acid contains N-acetylneuraminic acid, and the purity of bird's nest acid is not less than 98%.

[0045] Illustratively, in some embodiments of the present application, sialic acid contains N-acetylneuraminic acid, and the purity of sialic acid is 98%, 98.1%, 98.2%, 98.3%, 98.5%, 98.6%, 98.7%, 98.8%, 98.9%, 99%, 99.1%, 99.2%, 99.5% or a range between any two of the foregoing values.

[0046] Furthermore, in some embodiments of the present application, the bird's nest acid composition having whitening, brightening and anti-glycation functions includes: a pH regulator.

[0047] In some embodiments of the present application, the amount of the pH regulator in the bird's nest acid composition is 1 to 5 parts by weight.

[0048] For example, in some embodiments of the present application, the amount of the pH regulator in the bird's nest acid composition is 1 part, 2 parts, 3 parts, 4 parts, 5 parts or a range between any two of the foregoing values, calculated in parts by weight.

[0049] In the above technical solution, by adding a pH regulator to the bird's nest acid composition, on the one hand, the pH of the entire composition system can be adjusted and maintained in an appropriate pH range to stabilize niacinamide and effectively inhibit the formation of niacin; on the other hand, by adding a pH regulator, the pH of the composition system can be pre-adjusted to provide a compatible environment for the subsequent addition of ingredients.

[0050] Furthermore, in some embodiments of the present application, the pH regulator includes: at least one of an acidic regulator or an alkaline regulator; optionally, the pH regulator includes any one of arginine solution, citric acid solution, triethanolamine, sodium hydroxide solution or tromethamine.

[0051] For example, in some embodiments of the present application, the pH regulator can be selected from any one of an acid regulator or an alkaline regulator.

[0052] Further optionally, the pH adjuster includes at least one of arginine solution, citric acid solution, triethanolamine, sodium hydroxide solution or tromethamine.

[0053] For example, in some embodiments of the present application, the pH adjuster is selected from any one of arginine solution, citric acid solution, triethanolamine, sodium hydroxide solution, or tromethamine. Alternatively, in some embodiments of the present application, the pH adjuster is a mixture of arginine solution and citric acid solution; or in some embodiments of the present application, the pH adjuster is a mixture of triethanolamine and sodium hydroxide solution; or in some embodiments of the present application, the pH adjuster is a mixture of sodium hydroxide solution and tromethamine. The raw materials in the above mixtures can be mixed in any proportion.

[0054] Further optionally, in some embodiments of the present application, the mass percentage of the above-mentioned arginine solution, citric acid solution, and sodium hydroxide solution is 10% aqueous solution, so that the appropriate pH can be adjusted more quickly and stably to avoid pH mutation.

[0055] Furthermore, in some embodiments of the present application, the bird's nest acid composition with whitening, brightening and anti-glycation functions also includes: preservatives and antioxidants.

[0056] Furthermore, in some embodiments of the present application, in the bird's nest acid composition, the amount of antioxidant used is 0.1 parts to 5 parts; the amount of preservative used is 0.5 parts to 10 parts, calculated in parts by weight.

[0057] Illustratively, in some embodiments of the present application, in the bird's nest acid composition, the amount of antioxidant is 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 1 part, 1.5 parts, 2.0 parts, 2.5 parts, 3.0 parts, 3.5 parts, 4.0 parts, 4.5 parts, 5 parts or the range between any two of the foregoing values, and the amount of preservative is 0.5 part, 0.8 part, 0.9 part, 1 part, 1.5 parts, 2.0 parts, 2.5 parts, 3.0 parts, 3.5 parts, 2.0 parts, 10 parts or the range between any two of the foregoing values.

[0058] Furthermore, in some embodiments of the present application, the antioxidant includes at least one of sodium bisulfite, sodium sulfite or sodium metabisulfite.

[0059] Illustratively, in some embodiments of the present application, the antioxidant is: any one of sodium bisulfite, sodium sulfite or sodium metabisulfite; or in some embodiments of the application, the antioxidant is: a mixture of sodium bisulfite and sodium sulfite; or the antioxidant is: a mixture of sodium bisulfite, sodium sulfite and sodium metabisulfite; the individual raw materials in the above mixtures can be mixed in any proportion.

[0060] Furthermore, in some embodiments of the present application, the preservative includes: a water-soluble preservative.

[0061] Furthermore, in some embodiments of the present application, the preservative includes at least one of phenoxyethanol, ethylhexylglycerin, 1,2-pentanediol, 1,3-butanediol, 1,2-hexanediol or p-hydroxyacetophenone.

[0062] For example, in some embodiments of the present application, the preservative is any one of phenoxyethanol, ethylhexylglycerin, 1,2-pentanediol, 1,3-butylene glycol, 1,2-hexanediol or p-hydroxyacetophenone.

[0063] Some embodiments of the present application provide a method for preparing a bird's nest acid composition having whitening, brightening and anti-glycation functions, comprising: Pre-dissolving sialic acid and carnosine in water to obtain a first mixed solution; using a pH regulator to adjust the pH of the first mixed solution to 5.5-6.5 to obtain a second mixed solution; adding nicotinamide to the second mixed solution to dissolve and mix.

[0064] In the above technical solution, the bird's nest acid composition is innovatively prepared by a step-by-step dissolution process, which can achieve synergistic and stable loading of the three components. The resulting bird's nest acid composition composite system can significantly reduce the irritation and hydrolysis risk of niacinamide, and improve the transdermal absorption efficiency of bird's nest acid.

[0065] Furthermore, in the above technical solution, bird's nest acid and carnosine are pre-dissolved in water to obtain a first mixed solution; a pH regulator is used to adjust the pH of the first mixed solution to 5.5-6.5, and the pH regulator is used to stabilize nicotinamide and effectively inhibit the formation of nicotinic acid.

[0066] Illustratively, in some embodiments of the present application, the pH of the first mixed solution is adjusted to 5.5, 5.6, 5.7, 5.8, 5.9, 6.0, 6.1, 6.2, 6.3, 6.4 or 6.5 using the pH adjuster to obtain a second mixed solution.

[0067] Furthermore, in some embodiments of the present application, the pre-dissolution temperature is below 45°C.

[0068] Illustratively, in some embodiments of the present application, the pre-dissolution temperature is 45°C, 44°C, 43°C, 42°C, 41°C, 40°C, 39°C, 38°C, 37°C, 35°C or a range between any two of the foregoing values.

[0069] Furthermore, in some embodiments of the present application, after nicotinamide is added and dissolved and mixed, a pH adjuster is added again to adjust the pH of the mixture to 5.5-6.5.

[0070] For example, in some embodiments of the present application, after nicotinamide is added and dissolved and mixed, a pH adjuster is added again to adjust the pH of the mixture to 5.5, 5.6, 5.7, 5.8, 5.9, 6.0, 6.1, 6.2, 6.3, 6.4 or 6.5.

[0071] In the above technical solution, by adding nicotinamide and dissolving and mixing it, and then adding the pH regulator again, the pH of the system can be pre-adjusted to provide a compatible environment for the subsequent addition of ingredients.

[0072] In other optional embodiments of the present application, a preservative may be further added to the system prepared above.

[0073] For example, in some embodiments of the present application, the preservative may be selected from at least one of phenoxyethanol, ethylhexylglycerin, 1,2-pentanediol, 1,3-butanediol, 1,2-hexanediol or p-hydroxyacetophenone.

[0074] In other optional embodiments of the present application, antioxidants may be further added to the system prepared above.

[0075] For example, in some embodiments of the present application, the antioxidant may be selected from at least one of sodium bisulfite, sodium sulfite or sodium metabisulfite.

[0076] In some embodiments of the present application, a cosmetic is provided, comprising: a bird's nest acid composition with whitening, brightening and anti-glycation functions provided by any of the aforementioned embodiments.

[0077] Further optionally, in the above-mentioned cosmetics, the added amount of the bird's nest acid composition with whitening, brightening and anti-glycation functions is 0.1%-20% (w / w) in terms of volume percentage.

[0078] Illustratively, in some embodiments of the present application, in the above-mentioned cosmetics, the added amount of the bird's nest acid composition having whitening, brightening and anti-glycation functions is 0.1% (w / w), 0.2% (w / w), 0.5% (w / w), 0.8% (w / w), 1% (w / w), 1.2% (w / w), 1.5% (w / w), 1.8% (w / w), 2.0% (w / w), 3.0% (w / w), 5.0% (w / w), 10.0% (w / w), 15.0% (w / w), 18.0% (w / w), 19.0% (w / w), 20% (w / w) or the range between any two of the foregoing values, by volume percentage.

[0079] Furthermore, in some embodiments of the present application, the dosage form of the above-mentioned cosmetics includes any one of essence, lotion, facial mask, facial cream, eye cream or cleansing product.

[0080] Furthermore, in some embodiments of the present application, the above-mentioned cosmetic products are functional cosmetics. Accordingly, according to the dosage form of the product, different acceptable carrier ingredients, such as emulsifiers, moisturizers, oils, skin conditioners, etc., can be added according to actual needs.

[0081] The cosmetics provided herein can achieve whitening, brightening, and anti-glycation benefits, thereby fundamentally resolving skin problems. Furthermore, the whitening, brightening, and anti-glycation benefits include: increased tyrosinase inhibition, DPPH free radical scavenging, and inhibition of advanced glycation end products (AGEs) formation, as measured by ITA° values ​​in clinical human testing and satisfaction scores for improved skin condition in volunteers.

[0082] Furthermore, the above-mentioned cosmetics of this application, based on the concept of the same origin of makeup and food, optimize the synergistic combination of bird's nest acid, carnosine and niacinamide through scientific ratio, and construct a three-in-one whitening and anti-glycation pathway of "blocking glycation-inhibiting melanin-promoting metabolism"; the bird's nest acid composition prepared by the innovative step-by-step dissolution process can achieve a synergistic and stable loading of the three components. The resulting bird's nest acid composition composite system can significantly reduce the irritation and hydrolysis risk of niacinamide, and improve the transdermal absorption efficiency of bird's nest acid.

[0083] Furthermore, the above-mentioned cosmetics of the present application can simultaneously load bird's nest acid, carnosine and niacinamide in a specific optimized ratio, have good stability, high loading capacity, synergistic enhancement of effective ingredients, and significant whitening, brightening and anti-glycation effects; further, the above-mentioned cosmetics of the present application construct a composite system with bird's nest acid, carnosine and niacinamide, which can effectively reduce the content of niacinamide, reduce the irritation and hydrolysis risk it brings, and better exert its effect through the permeability of bird's nest acid.

[0084] The features and performance of the present invention are further described in detail below with reference to the embodiments: Example 1 This embodiment provides a whitening, brightening and anti-glycation bird's nest acid composition (I), which is composed of the following components in parts by weight: 5 parts of bird's nest acid, 10 parts of carnosine, 20 parts of niacinamide, and 65 parts of deionized water.

[0085] Preparation process: According to the step-by-step dissolution process, bird's nest acid and carnosine are first pre-dissolved in water at 40°C according to the above ratio, and the pH is adjusted to 6.0 using a pH regulator (10wt% arginine aqueous solution, 10wt% citric acid aqueous solution, 10wt% sodium hydroxide aqueous solution), and then niacinamide is added according to the above ratio and mixed and dissolved evenly.

[0086] Example 2 This embodiment provides a whitening, brightening and anti-glycation bird's nest acid composition (II), which is composed of the following components in parts by weight: 6 parts of bird's nest acid, 10 parts of carnosine, 24 parts of niacinamide, and 60 parts of deionized water.

[0087] Preparation process: According to the step-by-step dissolution process, bird's nest acid and carnosine are first pre-dissolved in water at 40°C according to the above ratio, and the pH is adjusted to 6.0 using a pH regulator (10wt% arginine aqueous solution, 10wt% citric acid aqueous solution, 10wt% sodium hydroxide aqueous solution), and then niacinamide is added according to the above ratio and mixed and dissolved evenly.

[0088] Example 3 This embodiment provides a whitening, brightening and anti-glycation bird's nest acid composition (III), which is composed of the following components in parts by weight: 4 parts of bird's nest acid, 9 parts of carnosine, 16 parts of niacinamide, and 71 parts of deionized water.

[0089] Preparation process: According to the step-by-step dissolution process, bird's nest acid and carnosine are first pre-dissolved in water at 40°C according to the above ratio, and the pH is adjusted to 6.0 using a pH regulator (10wt% arginine aqueous solution, 10wt% citric acid aqueous solution, 10wt% sodium hydroxide aqueous solution), and then niacinamide is added according to the above ratio and mixed and dissolved evenly.

[0090] Example 4 This embodiment provides a whitening, brightening and anti-glycation bird's nest acid composition (IV), which is composed of the following components in parts by weight: 5 parts of bird's nest acid, 11 parts of carnosine, 19 parts of niacinamide, and 65 parts of deionized water.

[0091] Preparation process: According to the step-by-step dissolution process, bird's nest acid and carnosine are first pre-dissolved in water at 40°C according to the above ratio, and the pH is adjusted to 6.0 using a pH regulator (10wt% arginine aqueous solution, 10wt% citric acid aqueous solution, 10wt% sodium hydroxide aqueous solution), and then niacinamide is added according to the above ratio and mixed and dissolved evenly.

[0092] Example 5 This embodiment provides a whitening, brightening and anti-glycation bird's nest acid composition (V), which is composed of the following components in parts by weight: 6 parts of bird's nest acid, 13 parts of carnosine, 25 parts of niacinamide, and 56 parts of deionized water.

[0093] Preparation process: According to the step-by-step dissolution process, bird's nest acid and carnosine are first pre-dissolved in water at 40°C according to the above ratio, and the pH is adjusted to 6.0 using a pH regulator (10wt% arginine aqueous solution, 10wt% citric acid aqueous solution, 10wt% sodium hydroxide aqueous solution), and then niacinamide is added according to the above ratio and mixed and dissolved evenly.

[0094] Comparative Example 1 This embodiment provides a whitening, brightening and anti-glycation bird's nest acid composition (a), which is composed of the following components in parts by weight: 5 parts of bird's nest acid, 20 parts of carnosine, 10 parts of niacinamide, and 65 parts of deionized water.

[0095] Preparation process: According to the step-by-step dissolution process, bird's nest acid and carnosine are first pre-dissolved in water at 40°C according to the above ratio, and the pH is adjusted to 6.0 using a pH regulator (10wt% arginine aqueous solution, 10wt% citric acid aqueous solution, 10wt% sodium hydroxide aqueous solution), and then niacinamide is added according to the above ratio and mixed and dissolved evenly.

[0096] Comparative Example 2 This embodiment provides a whitening, brightening and anti-glycation bird's nest acid composition (b), which is composed of the following components in parts by weight: 20 parts of bird's nest acid, 5 parts of carnosine, 10 parts of niacinamide, and 65 parts of deionized water.

[0097] Preparation process: According to the step-by-step dissolution process, bird's nest acid and carnosine are first pre-dissolved in water at 40°C according to the above ratio, and the pH is adjusted to 6.0 using a pH regulator (10wt% arginine aqueous solution, 10wt% citric acid aqueous solution, 10wt% sodium hydroxide aqueous solution), and then niacinamide is added according to the above ratio and mixed and dissolved evenly.

[0098] Comparative Example 3 This embodiment provides a whitening, brightening and anti-glycation bird's nest acid composition (c), which is composed of the following components in parts by weight: 20 parts of bird's nest acid, 10 parts of carnosine, 5 parts of niacinamide, and 65 parts of deionized water.

[0099] Preparation process: According to the step-by-step dissolution process, bird's nest acid and carnosine are first pre-dissolved in water at 40°C according to the above ratio, and the pH is adjusted to 6.0 using a pH regulator (10wt% arginine aqueous solution, 10wt% citric acid aqueous solution, 10wt% sodium hydroxide aqueous solution), and then niacinamide is added according to the above ratio and mixed and dissolved evenly.

[0100] Comparative Example 4 This embodiment provides a whitening, brightening and anti-glycation bird's nest acid composition (d), which is composed of the following components in parts by weight: 10 parts of bird's nest acid, 20 parts of carnosine, 5 parts of niacinamide, and 65 parts of deionized water.

[0101] Preparation process: According to the step-by-step dissolution process, bird's nest acid and carnosine are first pre-dissolved in water at 40°C according to the above ratio, and the pH is adjusted to 6.0 using a pH regulator (10wt% arginine aqueous solution, 10wt% citric acid aqueous solution, 10wt% sodium hydroxide aqueous solution), and then niacinamide is added according to the above ratio and mixed and dissolved evenly.

[0102] Comparative Example 5 This embodiment provides a whitening and brightening anti-glycation bird's nest acid composition (e), which is composed of the following components by weight: 10 parts of bird's nest acid, 5 parts of carnosine, 20 parts of niacinamide, and 65 parts of deionized water. Preparation process: According to the step-by-step dissolution process, bird's nest acid and carnosine are first pre-dissolved in water at 40°C according to the above ratio, and the pH is adjusted to 6.0 using a pH regulator (10wt% arginine aqueous solution, 10wt% citric acid aqueous solution, 10wt% sodium hydroxide aqueous solution), and then niacinamide is added according to the above ratio and mixed and dissolved evenly.

[0103] Comparative Example 6 This comparative example provides a bird's nest acid composition (f), which differs from Example I only in that the bird's nest acid component is missing, and the reduced number of parts is added to the deionized water in the ratio of the original number of parts, and other conditions remain unchanged.

[0104] Comparative Example 7 This comparative example provides a bird's nest acid composition (g), which differs from Example I only in that the carnosine component is missing, and the reduced number of parts is added to the deionized water in the ratio of the original number of parts, and other conditions remain unchanged.

[0105] Comparative Example 8 This comparative example provides a bird's nest acid composition (h), which differs from Example I only in that the niacinamide component is missing, and the reduced amount is added to the deionized water in the ratio of the original amount, while other conditions remain unchanged.

[0106] Comparative Example 9 This comparative example provides a bird's nest acid composition (i), which is composed of the following components in parts by weight: 35 parts of bird's nest acid and about 65 parts of deionized water.

[0107] Preparation process: According to the above ratio, bird's nest acid was evenly dissolved in water at 40°C, and the pH was adjusted to 6.0 using a pH adjuster (10wt% arginine aqueous solution, 10wt% citric acid aqueous solution, 10wt% sodium hydroxide aqueous solution).

[0108] Comparative Example 10 This comparative example provides a bird's nest acid composition (j), which is composed of the following components in parts by weight: 35 parts of carnosine and 65 parts of deionized water.

[0109] Carnosine was uniformly dissolved in water at 40° C. according to the above ratio, and the pH was adjusted to 6.0 using a pH adjuster (10 wt % arginine aqueous solution, 10 wt % citric acid aqueous solution, 10 wt % sodium hydroxide aqueous solution).

[0110] Comparative Example 11 This comparative example provides a bird's nest acid composition (k), which is composed of the following components in parts by weight: 35 parts of niacinamide and 65 parts of deionized water.

[0111] Nicotinamide was uniformly dissolved in water at 40° C. according to the above ratio, and the pH was adjusted to 6.0 using a pH adjuster (10 wt % arginine aqueous solution, 10 wt % citric acid aqueous solution, 10 wt % sodium hydroxide aqueous solution).

[0112] Application Example 1 This application example provides an essence with whitening, brightening and anti-glycation effects, which is composed of the following components in parts by weight: 10 parts of whitening, brightening and anti-glycation bird's nest acid composition (I), 0.7 parts of bentonite, 5 parts of butylene glycol, 0.2 parts of dipotassium glycyrrhizate, 2 parts of betaine, 0.2 parts of panthenol, 0.5 parts of p-hydroxyacetophenone, 0.5 parts of 1,2-hexanediol, and 80.9 parts of water.

[0113] Preparation process: Mix and dissolve the components uniformly at 40°C according to the above ratio.

[0114] Comparative Application Examples 1 to 11 This comparative application example provides 11 kinds of essences, which differ from Application Example 1 only in that the whitening, brightening and anti-glycation bird's nest acid composition (I) in Example 1 is replaced by the whitening, brightening and anti-glycation bird's nest acid composition (a~k) in the comparative example in equal amounts, and other conditions remain unchanged.

[0115] Test Example 1 Tyrosinase activity inhibition test was performed on the bird's nest acid compositions prepared in Examples 1 to 5 and Comparative Examples 1 to 11 Test instruments and reagents: Main instruments: Spectronic GENESYS-5 spectrophotometer, Thermo Fisher Scientific, USA.

[0116] Main reagents: tyrosinase, ≥1000 units / mg dry weight, Shanghai Tengzhun Biotechnology Co., Ltd.; levodopa 99%, RG Shanghai Titan Technology Co., Ltd.; sodium dihydrogen phosphate 98%, Shanghai Myrel Biochemical Technology Co., Ltd.; sodium dihydrogen phosphate 99%, RG Shanghai Titan Technology Co., Ltd.; bird's nest acid ≥98%, Beijing Green Kangcheng Biotechnology Co., Ltd.; nicotinamide 99%, Shanghai Baihaobo Biotechnology Co., Ltd.; carnosine ≥99%, Shanghai Puen Biochemical Technology Co., Ltd.

[0117] Reagent preparation: (1) Tyrosinase solution: Prepare the solution to a concentration of 100 μ / mL using PBS buffer at pH 6.8 and prepare immediately before use; (2) Levodopa solution: Prepare the solution to a concentration of 1 mg / mL using PBS buffer at pH 6.8 and store in a dark place.

[0118] Specific method: Add 1 mL of sample solution of the same concentration to each of the sample tube (T) and sample background (T0). Add 1 mL of PBS buffer to each of the enzyme reaction tube (C) and solvent background (C0). Add 0.5 mL of tyrosinase solution to each of the sample tube (T) and enzyme reaction tube (C). Replace the sample background (T0) and solvent background (C0) with 0.5 mL of PBS buffer. Mix the sample and tyrosinase thoroughly and incubate in a 37°C waterbath for 10 minutes. Add 2 mL of L-DOPA solution to each tube sequentially, maintaining a 5-minute reaction time for each tube. Immediately transfer each reaction solution to a cuvette and measure absorbance at 475 nm, using nicotinamide as a control. See Table 1 for details.

[0119] Table 1

[0120] The calculation formula of tyrosinase inhibition rate is shown as follows: Tyrosinase inhibition rate = 1-(AT-AT0) / (AC-AC0) × 100% Where AT, AT0, AC, and AC0 are the absorbance values ​​of T, T0, C, and C0, respectively.

[0121] The test results are shown in Table 2: Table 2

[0122] It can be seen from the data in Table 2 that Examples 1-5 exhibited better inhibitory efficacy in terms of tyrosinase inhibitory activity than Comparative Examples 1-11, which indicates that the synergistic effect and dose-effect relationship of the components in the examples of the present application have a key influence on optimizing the tyrosinase inhibition rate of the bird's nest acid composition.

[0123] It was further found that by comparing Examples 1-5 with Comparative Examples 1-5, it can be seen that when the ratios of Comparative Examples 1-5 are not within the scope of this application, the tyrosinase inhibition rate of Comparative Examples 1-5 is lower than that of the embodiment of this application, and the effect is reduced.

[0124] It was further found that when Examples 1-5 were compared with Comparative Examples 6-11, it can be seen that when the integrity of the bird's nest acid composition was impaired (any component was missing) or a single component was used (Comparative Examples 6-11), the tyrosinase inhibition effect showed a significant decrease. This further illustrates that the various components of the present application produce an excellent synergistic effect.

[0125] Test Example 2 The ABTS free radical scavenging rate test was performed on the bird's nest acid compositions prepared in Examples 1-5 and Comparative Examples 1-11; Test instruments and reagents: Main instruments: Spectronic GENESYS 5 spectrophotometer, Thermo Fisher Scientific, USA.

[0126] Main reagents: 2,2′-hydrazine-bis(3-ethylbenzothiazoline-6-sulfonic acid) diamine salt ≥99.5%, AR Shanghai MacLean Biochemical Technology Co., Ltd.; potassium persulfate ≥99%, Shanghai Titan Technology Co., Ltd.; sodium dihydrogen phosphate 98%, Shanghai Myrel Biochemical Technology Co., Ltd.; disodium hydrogen phosphate 99%, Shanghai Titan Technology Co., Ltd.; bird's nest acid ≥98%, Beijing Green Kangcheng Biotechnology Co., Ltd.; nicotinamide ≥99%, Shanghai Biohaobo Biotechnology Co., Ltd.; carnosine ≥99%, Shanghai Puen Biochemical Technology Co., Ltd.

[0127] Reagent preparation: (1) ABTS diammonium salt stock solution (74 mmol / L): Take 3 mg of ABTS diammonium salt and add 0.735 mL of distilled water (MW = 548.7); (2) Potassium persulfate (K2S2O8) stock solution (2.6 mmol / L): Take 1 mg of K2S2O8 and add 1.43 mL of distilled water (MW = 270.32); (3) Mix 0.2 mL of ABTS diammonium salt stock solution and 0.2 mL of K2S2O8 stock solution, place at room temperature in a dark environment for 12 hours, and dilute the mixture 10-20 times with PBS buffer (pH = 7.4) until the absorbance is 0.70 ± 0.02. This solution is the ABTS free radical working solution.

[0128] Specific method: Take 0.8 ml of ABTS free radical working solution and add a small amount of sample solution. Add the sample gradually, starting with a small amount and then gradually increasing it. Mix while adding and observe the discoloration of the solution. When the color of the solution has mostly faded, record the amount of sample added. Set the previous three amounts to form an arithmetic progression. See Table 3 for details.

[0129] Table 3

[0130] A value: Mix the reagents evenly, keep them at room temperature in the dark for 30 min, and measure the A value using a spectrophotometer at 734 nm. Measure the absorbance three times in parallel and take the average value.

[0131] The calculation formula for ABTS clearance (%) is as follows: ABTS clearance (%) = 1-(A1-A2) / A0×100% The test results are shown in Table 4: Table 4

[0132] It can be seen from the data in Table 4 that Examples 1-5 exhibited better inhibitory efficacy in terms of ABTS free radical scavenging rate than Comparative Examples 1-11, which suggests that the synergistic effect and dose-effect relationship of each component have a key influence on optimizing the ABTS free radical scavenging rate of the bird's nest acid composition.

[0133] It was further found that by comparing Examples 1-5 with Comparative Examples 1-5, it can be seen that when the ratios of Comparative Examples 1-5 are not within the scope of this application, the ABTS free radical scavenging rates of Comparative Examples 1-5 are lower than those of the embodiments of this application, and the effects are reduced.

[0134] It was further found that when the integrity of the swalnic acid composition was impaired (any component was missing) or a single component was used (Comparative Examples 6-11), the ABTS free radical scavenging rate showed a significant downward trend. This further illustrates that the various components of the present application produce an excellent synergistic effect.

[0135] Test Example 3 The sialic acid compositions prepared in Examples 1-5 and Comparative Examples 1-11 were subjected to an experiment on the inhibition rate of advanced glycation end products (AGEs). Test instruments and reagents: Main instruments: Spectronic GENESYS 5 spectrophotometer, Thermo Fisher Scientific, USA.

[0136] Main reagents: L-(+)-arabinose 99%, Shanghai Yuanye Biotechnology Co., Ltd.; L-lysine 99%, Shanghai Myrrel Biochemical Technology Co., Ltd.; sodium dihydrogen phosphate 98%, Shanghai Myrrel Biochemical Technology Co., Ltd.; sodium hydrogen phosphate 99%, Shanghai Titan Technology Co., Ltd.; bird's nest acid ≥98%, Beijing Green Kangcheng Biotechnology Co., Ltd.; nicotinamide ≥99%, Shanghai Baihaobo Biotechnology Co., Ltd.; carnosine ≥99%, Shanghai Puen Biochemical Technology Co., Ltd.

[0137] Specific method: L-(+)-arabinose (1 mmol / L) and lysine (10 mmol / L) were dissolved in 200 mmol / L PBS buffer (pH 7.4) containing 3 mmol / L sodium azide. Sample solutions of varying concentrations were added, with a control group without sample added serving as the blank control. The remaining groups served as experimental groups. The reaction was incubated in a 90°C water bath for 8 h. All experiments were performed in triplicate. Based on the fluorescence characteristic of AGEs, which emit an emission wavelength of 440 nm under excitation at 370 nm, the fluorescence intensity of the reaction solution was measured on a fluorescence spectrometer. The fluorescence spectrometer parameters were set to an excitation wavelength of 370 nm, a slit of 1 nm, an emission wavelength of 440 nm, and a slit of 5 nm. See Table 5 for details.

[0138] Table 5

[0139] Since AGEs have the fluorescence property of producing an emission wavelength of 440 nm under 370 nm excitation light, the fluorescence value was measured on a fluorescence spectrometer and the inhibition rate of AGEs formation was calculated according to the following formula.

[0140] Advanced glycation end products AGEs inhibition rate = 1- F1 / F0 × 100%; The test results are shown in Table 6: Table 6

[0141] It can be seen from the data in Table 6 that Examples 1-5 show better inhibitory efficacy in terms of the inhibition rate of advanced glycation end products AGEs than Comparative Examples 1-11, which suggests that the synergistic effect and dose-effect relationship of each component have a key influence on optimizing the AGEs inhibition rate of the bird's nest acid composition.

[0142] It was further found that by comparing Examples 1-5 with Comparative Examples 1-5, it can be seen that when the ratios of Comparative Examples 1-5 are not within the scope of this application, the AGEs inhibition rate of Comparative Examples 1-5 is lower than that of the embodiments of this application, and the effect is reduced.

[0143] It was further found that when Examples 1-5 were compared with Comparative Examples 6-11, it was found that when the integrity of the bird's nest acid composition was impaired (any component was missing) or a single component was used (Comparative Examples 6-11), the AGE inhibition rate showed a significant downward trend. This further illustrates that the various components of the present application produce an excellent synergistic effect.

[0144] Test Example 4 Transdermal absorption performance test of the bird's nest acid compositions prepared in Examples 1-5 and Comparative Examples 1-11 Test instruments and reagents: Main instruments: TK-12D transdermal diffusion tester, Shanghai Kaikai Technology Trading Co., Ltd.; high performance liquid chromatograph (UV + evaporative light scattering) (LC-20ADXR), Shimadzu (Shanghai) Laboratory Equipment Co., Ltd.

[0145] Main reagents: analytical grade methanol, Sinopharm Chemical Reagent Co., Ltd.; sodium dihydrogen phosphate 98%, Shanghai Myrel Biochemical Technology Co., Ltd.; disodium hydrogen phosphate 99%, RG Shanghai Titan Technology Co., Ltd.; N-acetylneuraminic acid ≥98%, Beijing Green Kangcheng Biotechnology Co., Ltd.; nicotinamide ≥99%, Shanghai Biohobo Biotechnology Co., Ltd.; carnosine ≥99%, Shanghai Puen Biochemical Technology Co., Ltd.

[0146] Specific method: Undamaged pig skin of comparable thickness was selected for transdermal testing. First, subcutaneous fat was removed from the pig skin using a scalpel until the pores were clear. Any remaining hair on the pig skin surface was plucked with tweezers. The pig skin was then cut into approximately 2.5 cm × 2.5 cm pieces and fixed between the receiving and supply cells of a Franz diffusion cell, with the dermis facing the receiving cell. The receiving cell was filled with phosphate buffered saline (PBS) at pH 7.4. 1 mL of the 5% by mass solution of the bird's nest acid composition of Examples 1-5 and Comparative Examples 1-11 was added to the supply cell. The assembled Franz diffusion cell was placed in a transdermal diffusion apparatus. The experimental conditions were: temperature 37°C, stirring speed 400 rpm, and transdermal time 24 hours. After 24 hours, the pigskin was removed from the diffusion cell, and the residual sample on the surface of the pigskin was wiped off with absorbent cotton. The pigskin was chopped and placed in a 20 mL centrifuge tube. 5 mL of methanol was added and ultrasonicated for 30 minutes. Then, the tube was centrifuged at 5000 r / min for 30 minutes. The supernatant was filtered with a 0.22 μm organic filter membrane and the content of the active ingredients sialic acid, carnosine and niacinamide in the sialic acid composition was determined by high performance liquid chromatography, which is the total content of the active ingredients in the stratum corneum and dermis.

[0147] The test results are shown in Table 7: Table 7

[0148] It can be seen from the data in Table 7 that Examples 1-5 exhibit better transdermal absorption than Comparative Examples 1-11, which suggests that the synergistic effect and dose-effect relationship of each component have a key influence on optimizing the transdermal absorption of the bird's nest acid composition.

[0149] It was further found that by comparing Examples 1-5 with Comparative Examples 1-5, it can be seen that when the ratios of Comparative Examples 1-5 are not within the scope of this application, the transdermal absorbability of Comparative Examples 1-5 is lower than that of the embodiment of this application, and the effect is reduced.

[0150] It was further found that, by comparing Examples 1-5 with Comparative Examples 6-11, it can be seen that when the integrity of the bird's nest acid composition was impaired (any component was missing) or a single component was used (Comparative Examples 6-11), the transdermal absorbability showed a significant downward trend. This further illustrates that the various components of the present application produce an excellent synergistic effect.

[0151] Test Example 5 Safety tests were performed on the essences prepared from Example 1 and Comparative Application Examples 1-11.

[0152] Schematic diagram of the essence Figure 1 shown.

[0153] Specific method: Select qualified patch test equipment and use the closed patch test method. Place 0.020mL~0.025mL of the test substance in the patch tester, and apply it to the flexor side of the subject's forearm with hypoallergenic tape. Remove the test substance after 24 hours, and observe the skin reaction 0.5, 24, and 48 hours after removal. Record the results according to the skin reaction grading standards in the "Technical Specifications for Safety of Cosmetics" (2015 edition).

[0154] Skin reaction grading standards: see Table 8.

[0155] Table 8

[0156] The results showed that the skin reactions of the subjects were all negative, indicating that the bird's nest acid composition and essence involved in the present invention are mild, safe and non-irritating.

[0157] Test Example 5 The whitening effect of the essence prepared from Example 1 and Comparative Application Examples 1-11 was tested.

[0158] The specific method was to select 10 volunteers (5 males, 5 females) aged 20-50 years, half male and half female, for each test substance. Post-use testing was performed using a multifunctional skin tester (model: MPA580) manufactured by CourageKhazaka, Germany. The essences prepared in Application Example 1 and Comparative Application Examples 1-11 were applied to the facial skin twice daily for 28 days. The ITA values ​​(average) of each group of volunteers were measured on Day 0 and Day 28 to evaluate the whitening effects of the test substances. The average ITA values ​​are shown in Table 9: Table 9

[0159] It can be seen from the data in Table 9 that Application Example 1 shows a better effect in brightening the skin tone than Comparative Application Examples 1-11, which suggests that the synergistic effect and dose-effect relationship of each component have a key influence on optimizing the skin tone brightening effect of the bird's nest acid composition.

[0160] Further, the human body efficacy test example diagram of the essence prepared in Example 1 is shown, using facial skin photos at 0D and 28D as shown in FIG. Figure 2 As shown in the figure, it can be seen that compared with using 0D, the skin tone uniformity and radiance are significantly improved after using 28D.

[0161] It was further found that when the ratios of Examples 1-5 were outside the scope of the present application, or when the integrity of the bird's nest acid composition was impaired (any component was missing) or a single component was used, the skin-lightening effect was reduced to a certain extent. This further demonstrates that the various components of the present application produce an excellent synergistic effect.

[0162] Test Example 6 The skin improvement satisfaction scores were evaluated for the essences prepared from Example 1 and Comparative Application Examples 1-11.

[0163] The specific method is as follows: 150 female volunteers aged 25-45 were selected and divided into 15 groups. The essences prepared in Application Example 1 and Comparative Application Examples 1-11 were applied to the facial skin twice a day for 4 weeks. The volunteers evaluated and scored the skin care products based on their skin improvement. The average of the scores was used as the final result. The scoring criteria are shown in Table 10 below: Table 10

[0164] The average scores of volunteers after trying the experimental products for 4 consecutive weeks are shown in Table 11: Table 11

[0165] From the scoring results in Table 11, it can be seen that Application Example 1 shows a better effect in terms of overall skin improvement satisfaction than Comparative Application Examples 1-11, which suggests that the synergistic effect and dose-effect relationship of each component have a key influence on optimizing the efficacy of the bird's nest acid composition.

[0166] It was further found that when the ratios of Examples 1-5 were outside the scope of this application, or when the integrity of the bird's nest acid composition was impaired (any component was missing) or a single component was used, the skin improvement satisfaction decreased to a certain extent. This further illustrates that the various components of this application produce an excellent synergistic effect.

[0167] The above description is merely a preferred embodiment of the present application and is not intended to limit the present application. Various modifications and variations are possible for those skilled in the art. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present application shall be included within the scope of protection of the present application.

Claims

1. A bird's nest acid composition with whitening, brightening and anti-glycation functions, characterized in that: Calculated by weight, the bird's nest acid composition comprises: 4-10 parts of bird's nest acid, 8-15 parts of carnosine and 15-25 parts of niacinamide.

2. The bird's nest acid composition with whitening, brightening and anti-glycation functions according to claim 1, characterized in that: Calculated by weight, the bird's nest acid composition comprises: 4-8 parts of bird's nest acid, 8-12 parts of carnosine and 15-24 parts of niacinamide.

3. The bird's nest acid composition with whitening, brightening and anti-glycation functions according to claim 1, characterized in that: Calculated by weight, the bird's nest acid composition comprises: 4-6 parts of bird's nest acid, 8-12 parts of carnosine, and 15-24 parts of niacinamide.

4. The bird's nest acid composition with whitening, brightening and anti-glycation functions according to claim 1, characterized in that In the bird's nest acid composition, the mass ratio of the bird's nest acid to the carnosine is 0.27-1.

25.

5. The bird's nest acid composition with whitening, brightening and anti-glycation functions according to claim 1, characterized in that: In the bird's nest acid composition, the mass ratio of the bird's nest acid to the niacinamide is 0.16-0.

67.

6. The bird's nest acid composition with whitening, brightening and anti-glycation functions according to claim 1, characterized in that: In the bird's nest acid composition, the mass ratio of the carnosine to the niacinamide is 0.32-1.

00.

7. The bird's nest acid composition with whitening, brightening and anti-glycation functions according to any one of claims 1 to 6, characterized in that: The bird's nest acid composition with whitening, brightening and anti-glycation functions includes a pH regulator; In parts by weight, the amount of the pH regulator in the bird's nest acid composition is 1 part to 5 parts; Optionally, the pH regulator includes: at least one of an acidic regulator or an alkaline regulator; Optionally, the pH regulator includes at least one of arginine solution, citric acid solution, triethanolamine, sodium hydroxide solution or tromethamine.

8. The bird's nest acid composition with whitening, brightening and anti-glycation functions according to claim 1, characterized in that: The bird's nest acid composition with whitening, brightening and anti-glycation functions also includes: antioxidants and preservatives; Optionally, in the bird's nest acid composition, the amount of the preservative is 0.5-10 parts; the amount of the antioxidant is 0.1-5 parts; Optionally, the antioxidant includes: at least one of sodium bisulfite, sodium sulfite or sodium metabisulfite; Optionally, the preservative includes: a water-soluble preservative; optionally, the preservative includes: at least one of phenoxyethanol, ethylhexylglycerin, 1,2-pentanediol, 1,3-butanediol, 1,2-hexanediol or p-hydroxyacetophenone.

9. The method for preparing the bird's nest acid composition having whitening, brightening and anti-glycation functions according to claim 8, characterized in that: include: Pre-dissolving the scutellaria baicalensis and carnosine in water to obtain a first mixed solution; adjusting the pH of the first mixed solution to 5.5-6.5 using a pH adjuster to obtain a second mixed solution; adding the nicotinamide to the second mixed solution to dissolve and mix; Optionally, the pre-dissolution temperature is below 45°C; Optionally, after adding the nicotinamide and dissolving and mixing, the pH adjuster is added again to make the pH of the mixed solution 5.5-6.

5.

10. A cosmetic, characterized in that: The cosmetic comprises: the bird's nest acid composition with whitening, brightening and anti-glycation functions according to any one of claims 1 to 8; Optionally, in the cosmetic, the added amount of the bird's nest acid composition with whitening, brightening and anti-glycation functions is 0.1%-20% (w / w) in terms of volume percentage.

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