Valve integration module, liquid path system and gene sequencer

Abstract

The invention relates to the technical field of gene sequencing equipment, in particular to a valve integration module, a liquid path system and a gene sequencer. According to the valve integration module, the multiple flow channel units are integrated together, the common flow channel can be communicated with different main liquid inlets by switching the state of the valve element, and then different reagents can enter the common flow channel. Due to the fact that each flow channel unit is provided with a valve structure, different flow channel units can be independently controlled according to needs, and different common flow channels can supply different reagents. The length of the pipeline can be shortened through the valve integration module, so that the loss of the reagent is reduced, and the technical problem of high reagent loss of an existing liquid path system is solved.

Description

Technical Field

[0001] The present invention relates to the technical field of gene sequencing equipment, and in particular to a valve integrated module, a liquid circuit system and a gene sequencer. Background Art

[0002] Gene sequencing equipment can identify and sort the base sequences of sample DNA. Current sequencers typically include a fluidic system and a chip platform. The sequencing chip is mounted on the chip platform, and the fluidic system supplies reagents or samples to the sequencing chip. Currently, fluidic systems often use pipelines for fluid transmission, with valves arranged on the pipelines to control the opening and closing of the fluidic circuits. When different reagents need to be supplied to multiple pipelines separately, multiple pipe joints are required to form a manifold, which makes the fluidic system more complex and extends the length of the pipelines, resulting in a large amount of reagent loss. Summary of the Invention

[0003] The present invention provides a valve integrated module for improving the technical problem of large reagent loss in the current liquid circuit system.

[0004] In addition, the present invention also aims to provide a gene sequencer using the above-mentioned valve integrated module.

[0005] In addition, the present invention also aims to provide a fluid circuit system using the above valve integrated module.

[0006] According to the first aspect, an embodiment provides a valve integrated module, wherein the valve integrated module has a first main liquid inlet and a second main liquid inlet, and the valve integrated module includes at least two flow channel units; the flow channel unit includes:

[0007] a first branch flow channel, the first branch flow channel being connected to the first main liquid inlet,

[0008] a second branch flow channel, the second branch flow channel being in communication with the second main liquid inlet;

[0009] A common flow channel, wherein the common flow channel has a flow channel outlet;

[0010] And a valve structure, the valve structure has a first valve inlet, a second valve inlet and a valve outlet, the valve outlet is connected to the common flow channel, the first valve inlet is connected to the first branch flow channel, and the second valve inlet is connected to the second branch flow channel; the valve structure includes a valve core, the valve core has a first state and a second state; when the valve core is in the first state, the first branch flow channel is connected to the common flow channel and the second branch flow channel is disconnected from the common flow channel; when the valve structure is in the second state, the second branch flow channel is connected to the common flow channel and the first branch flow channel is disconnected from the common flow channel.

[0011] Further, in one embodiment, the valve integrated module has a first manifold flow channel, the first manifold flow channel is connected to each of the first branch flow channels, and the first main liquid inlet is connected to each of the first branch flow channels through the first manifold flow channel; and / or the valve integrated module has a second manifold flow channel, the second manifold flow channel is connected to each of the second branch flow channels, and the second main liquid inlet is connected to each of the second branch flow channels through the second manifold flow channel.

[0012] Furthermore, in one embodiment, the first manifold flow channel includes a main flow channel and a secondary flow channel, the secondary flow channel is connected to at least two of the first branch flow channels, the main flow channel is connected to at least two of the secondary flow channels, or the main flow channel is connected to at least one of the secondary flow channels and at least one of the first branch flow channels.

[0013] Furthermore, in one embodiment, each of the first branch flow channels and each of the second branch flow channels extends along the first direction, each of the first branch flow channels is arranged along the second direction to form a first branch flow channel group, and each of the second branch flow channels is arranged along the second direction to form a second branch flow channel group, and the first branch flow channel group and the second branch flow channel group are arranged in layers in a third direction, and the first direction, the second direction and the third direction are respectively the extension directions of the three coordinate axes in the three-dimensional coordinate system.

[0014] Furthermore, in one embodiment, the common flow channel extends along the first direction, and each common flow channel is arranged along the second direction to form a common flow channel group, and the first branch flow channel group, the common flow channel group and the second branch flow channel group are arranged in layers in the third direction; in the same flow channel unit: the common flow channel is between the first branch flow channel and the second branch flow channel in the third direction.

[0015] Furthermore, in one embodiment, the valve integrated module has a first manifold flow channel, which is connected to the first branch flow channel in each of the flow channel units, and the first main liquid inlet is connected to each of the first branch flow channels through the first manifold flow channel; the valve integrated module has a second manifold flow channel, which is connected to the second branch flow channel in each of the flow channel units, and the second main liquid inlet is connected to each of the second branch flow channels through the second manifold flow channel; the first manifold flow channel and the second manifold flow channel are arranged in layers in the first direction.

[0016] Furthermore, in one embodiment, the valve integrated module includes a valve block and a reversing valve fixed on the valve block, the reversing valve forms the valve structure, the valve block has a reversing valve mounting surface for mounting the reversing valve, and the outlet of the first branch flow channel, the outlet of the second branch flow channel and the inlet of the common flow channel are all located on the reversing valve mounting surface.

[0017] Furthermore, in one embodiment, the valve block has a liquid outlet side surface opposite to the installation surface of the reversing valve, and the flow channel outlet is arranged on the liquid outlet side surface.

[0018] Furthermore, in one embodiment, the valve block further has a liquid inlet side surface, the liquid inlet side surface connects the liquid outlet side surface and the reversing valve mounting surface, and the first main liquid inlet and the second main liquid inlet are located on the liquid inlet side surface.

[0019] Furthermore, in one embodiment, each of the valve cores is an electrically controlled valve core that can be independently controlled.

[0020] In a second aspect, an embodiment provides a fluid system comprising a pipeline and a valve integrated module as described in any embodiment of the first aspect.

[0021] In a third aspect, a gene sequencer is provided, comprising a liquid system and a sequencing chip carrying platform, wherein the liquid system comprises a pipeline and a valve integrated module as described in any one of the embodiments of the first aspect.

[0022] Furthermore, in one embodiment, the flow channel outlet is used to communicate with a sequencing flow channel inlet on a sequencing chip used by the gene sequencer;

[0023] The liquid circuit system includes a first rotary valve and a second rotary valve, wherein the first rotary valve has a first rotary valve liquid outlet and a plurality of first rotary valve liquid inlets, wherein the first rotary valve liquid inlet is used to communicate with the synthesis sequencing reagent set in the reagent kit and selectively allow the target reagent in the synthesis sequencing reagent set to flow out from the first rotary valve liquid outlet; the first rotary valve liquid outlet is communicated with the first main liquid inlet;

[0024] The second rotary valve has a second rotary valve liquid outlet and multiple second rotary valve liquid inlets. The second rotary valve liquid inlet is used to communicate with the auxiliary reagent group in the reagent kit and select to allow the target reagent in the auxiliary reagent group to flow out from the second rotary valve liquid outlet; the second rotary valve liquid outlet is connected to the second main liquid inlet.

[0025] According to the above embodiments, in the valve integration module of the present application, multiple flow channel units are integrated together, and by switching the state of the valve core, the common flow channel can be connected to different main liquid inlets, thereby enabling different reagents to enter the common flow channel. Since each flow channel unit is equipped with a valve structure, different flow channel units can be individually controlled as needed, so that different common flow channels can supply different reagents. The present application can shorten the length of the pipeline through the valve integration module, thereby reducing the loss of reagents, and improving the technical problem of large reagent loss in the current liquid circuit system. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 This is a schematic diagram of the principle of a liquid circuit system in one embodiment;

[0027] Figure 2 A schematic structural diagram of a valve block in a valve integration module in an embodiment;

[0028] Figure 3 Another structural diagram of a valve block in a valve integration module in an embodiment;

[0029] Figure 4 A bottom view of a valve block in a valve integration module in one embodiment;

[0030] Figure 5 For the Figure 4 Cross-sectional view of AA;

[0031] Figure 6 A front view of a valve integration module in one embodiment;

[0032] Figure 7 For the Figure 6 Cross-sectional view of the middle BB;

[0033] Figure 8 For the Figure 6 Cross-sectional view of CC.

[0034] List of feature names corresponding to the reference numerals in the figure: 1. First main liquid inlet; 2. Second main liquid inlet; 3. Flow channel unit; 31. First branch flow channel; 311. Outlet; 32. Second branch flow channel; 321. Outlet; 33. Common flow channel; 331. Flow channel outlet; 332. Inlet; 34. Valve structure; 340. Reversing valve; 341. First valve inlet; 342. Second valve inlet; 343. Valve outlet; 4. Valve block; 41. Reversing valve mounting surface; 42. Reversing valve fixing hole; 43. Liquid outlet side; 44. Liquid inlet side; 5. First manifold flow channel; 51. First main flow channel; 52. First secondary flow channel; 521. First secondary flow channel Stage; 522, first end section; 6, second manifold flow channel; 61, second main channel; 62, second secondary flow channel; 621, second stage; 622, second end section; 7, liquid path system; 71, first rotary valve; 711, first rotary valve liquid outlet; 712, first rotary valve liquid inlet; 72, second rotary valve; 721, second rotary valve liquid outlet; 722, second rotary valve liquid inlet; 8, sequencing chip; 81, sequencing flow channel; 811, sequencing flow channel liquid inlet; 9, reagent kit; 91, synthesis sequencing reagent set; 92, auxiliary reagent set; 10, liquid outlet block; 11, injection pump; 12, confluence block; 13, waste liquid barrel.

[0035] Explanation of the reference numerals in brackets in the accompanying drawings: In the reference numerals in brackets in the accompanying drawings, the features referred to by the reference numerals are both the features represented by the numbers in the brackets and the features represented by the numbers outside the brackets. DETAILED DESCRIPTION

[0036] The present invention will be further described in detail below by means of specific embodiments in conjunction with the accompanying drawings. Similar elements in different embodiments are numbered with associated similar elements. In the following embodiments, many detailed descriptions are provided to enable the present application to be better understood. However, those skilled in the art will readily appreciate that some of the features may be omitted in different circumstances, or may be replaced by other elements, materials, or methods. In some cases, some operations related to the present application are not shown or described in the specification. This is to avoid the core portion of the present application being overwhelmed by excessive descriptions, and for those skilled in the art, it is not necessary to describe these related operations in detail. They will fully understand the related operations based on the description in the specification and the general technical knowledge in the art.

[0037] In addition, the features, operations, or characteristics described in the specification may be combined in any appropriate manner to form various embodiments. Furthermore, the steps or actions in the method description may be reordered or adjusted in a manner readily apparent to those skilled in the art. Therefore, the various sequences in the specification and drawings are provided solely for the purpose of clearly describing a particular embodiment and are not intended to be mandatory, unless otherwise specified.

[0038] The serial numbers assigned to components herein, such as "first," "second," etc., are used solely to distinguish the objects being described and do not convey any sequential or technical meaning. References to "connection" and "coupling" herein, unless otherwise specified, include both direct and indirect connections (couplings).

[0039] Current manifold-based fluidic systems are complex, with long shared pipes connecting different branches. This leads to significant reagent loss when used in gene sequencing. This application provides a valve integration module that integrates valve structures and flow channels within the module. This reduces the length of the pipes while enabling the simultaneous supply of different reagents to sequencing chips, thereby alleviating the significant reagent loss associated with current fluidic systems.

[0040] The valve integrated module and the fluid circuit system in this application are described in detail below with reference to the accompanying drawings.

[0041] In one embodiment, please refer to Figures 1 to 8 The valve integrated module has a first main liquid inlet 1 and a second main liquid inlet 2. Both the first main liquid inlet 1 and the second main liquid inlet 2 can be used to allow liquid to enter the valve integrated module.

[0042] The valve integrated module includes at least two flow channel units 3, each including a first branch flow channel 31 and a second branch flow channel 32. The first branch flow channel 31 communicates with the first main liquid inlet 1, allowing liquid entering the valve integrated module through the first main liquid inlet 1 to enter the first branch flow channel 31. The second branch flow channel 32 communicates with the second main liquid inlet 2, allowing liquid entering the valve integrated module through the second main liquid inlet 2 to enter the second branch flow channel 32.

[0043] The flow channel unit 3 also includes a common flow channel 33 and a valve structure 34. The liquid flowing out of the first branch flow channel 31 and the liquid flowing out of the second branch flow channel 32 need to pass through the valve structure 34 before entering the common flow channel 33 from the valve structure 34. The common flow channel 33 has a flow channel outlet 331, and the flow channel outlet 331 is used to allow the fluid to flow out of the common flow channel 33. The liquid in the first branch flow channel 31 and the second branch flow channel 32 can both enter the common flow channel 33. Since the liquid in the first branch flow channel 31 and the liquid in the second branch flow channel 32 need to enter the common flow channel 33 separately, the valve structure 34 is used in this application to switch the communication state between the common flow channel 33 and the first branch flow channel 31 and the second branch flow channel 32.

[0044] The valve structure 34 has a first valve inlet 341, a second valve inlet 342, and a valve outlet 343. The valve outlet 343 is connected to the common flow channel 33, so that the fluid entering the valve structure 34 enters the common flow channel 33. The first valve inlet 341 is connected to the first branch flow channel 31, so that the fluid in the first branch flow channel 31 enters the valve structure 34 through the first valve inlet 341. The second valve inlet 342 is connected to the second branch flow channel 32, so that the fluid in the second branch flow channel 32 enters the valve structure 34 through the second valve inlet 342. The valve structure 34 includes a valve core (not shown in the figure), which has a first state and a second state. By switching the state of the valve core, the connection state of the first valve inlet 341, the second valve inlet 342, and the valve outlet 343 can be changed, so that the first branch flow channel 31 or the second branch flow channel 32 is connected to the common flow channel 33. When the valve core is in the first state, the first branch flow channel 31 is connected to the common flow channel 33 and the second branch flow channel 32 is disconnected from the common flow channel 33. When the valve structure 34 is in the second state, the second branch flow channel 32 is connected to the common flow channel 33 and the first branch flow channel 31 is disconnected from the common flow channel.

[0045] The valve integrated module in the present application integrates at least two flow channel units 3. The first branch flow channel 31 of each flow channel unit 3 is communicated with the first main liquid inlet 1, and the second branch flow channel 32 of each flow channel unit 3 is communicated with the second main liquid inlet 2. In this way, the liquid entering through the first main liquid inlet 1 can flow to the first branch flow channel 31 of each flow channel unit 3, and the liquid entering through the second main liquid inlet 2 can flow to the second branch flow channel 32 of each flow channel unit 3. Different types of liquids can be input through the first main liquid inlet 1 and the second main liquid inlet 2.

[0046] Since the first branch channel 31 and the second branch channel 32 can both communicate with the common channel 33, the control valve structure 34 can control the conduction state of different branch channels and the common channel 33. In this way, for the common channels 33 of different channel units 3, different types of liquids can flow out of the channel outlet 331. Of course, according to the needs of use, the same type of liquid can also flow out of the channel outlet 331. Compared with the current solution of using manifold pipelines to share different types of liquids, the present application integrates the branch channels and the common channel 33 on the valve integrated module, which greatly simplifies the structure of the liquid circuit, shortens the length of the liquid circuit, and thus reduces the loss of reagents.

[0047] The valve integrated module can be formed using any feasible molding method, such as injection molding, casting, or by joining and fixing multiple valve blocks. The valve structure 34 in the valve integrated module can also be formed using any feasible method. The valve structure 34 can be integrated within the valve block, or directly fixed to the valve block using a mature valve in the prior art. Using a mature valve can obviously reduce design difficulty and requirements, facilitating the manufacture of the valve integrated module.

[0048] Specifically, in one embodiment, please refer to Figures 1 to 3 The valve integrated module includes a valve block 4 and a reversing valve 340 fixed to the valve block 4. The reversing valve 340 forms a valve structure 34. The valve block 4 has a reversing valve mounting surface 41 for mounting the reversing valve 340. The outlet 311 of the first branch channel 31, the outlet 321 of the second branch channel 32, and the inlet 332 of the common channel 33 are all located on the reversing valve mounting surface 41. The integration of the reversing valve 340 on the valve block 4 can reduce pipeline volume, lower reagent consumption, and conserve reagents.

[0049] Specifically, the valve block 4 has a reversing valve fixing hole 42 for fixing the reversing valve 340. The reversing valve 340 is fixed to the valve block 4 by a screw screwed into the reversing valve fixing hole 42. In some other embodiments, the reversing valve 340 can be fixed to the valve block 4 using any feasible method, such as clamp fixation, welding fixation, adhesive fixation, etc.

[0050] For further information, please refer to Figures 1 to 3The valve block 4 has a liquid outlet side surface 43 opposite to the reversing valve mounting surface 41, and the flow channel outlet 331 is arranged on the liquid outlet side surface 43. Since the flow channel outlet 331 needs to be connected to other components, arranging the flow channel outlet 331 on the liquid outlet side surface 43 provides more space, facilitates the connection of the valve integrated module with other components, and is less likely to interfere with the reversing valve 340.

[0051] Of course, in some other embodiments, the reversing valve 340 and the flow channel outlet 331 may also be arranged on two adjacent side surfaces of the valve block 4 .

[0052] Furthermore, in one embodiment, please refer to Figures 1 to 3 The valve block 4 also has a liquid inlet side surface 44, which connects the liquid outlet side surface 43 and the reversing valve mounting surface 41. The first main liquid inlet 1 and the second main liquid inlet 2 are located on the liquid inlet side surface 44. The arrangement of the liquid inlet side surface 44, the liquid outlet side surface 43, and the reversing valve mounting surface 41 facilitates connection with other components and enables more efficient use of the space surrounding the valve assembly module. In other embodiments, as needed, at least two of the first main liquid inlet 1, the second main liquid inlet 2, the flow channel outlet 331, and the reversing valve 340 can also be arranged on the same side surface of the valve block 4.

[0053] To facilitate control of the valve cores, in one embodiment, each valve core is an independently controllable electrically controlled valve core. In one embodiment, the reversing valve 340 is a solenoid valve. This electrically controlled valve core facilitates automated control and improves the efficiency of the hydraulic system. In one embodiment, the reversing valve can also be a motor-driven electrically controlled valve.

[0054] In order to facilitate the communication between the first main liquid inlet 1 and the first branch flow channel 31 in each flow channel unit 3, in one embodiment, please refer to Figure 7 The valve integrated module has a first manifold flow channel 5 , which is connected to each first branch flow channel 31 , and the first main liquid inlet 1 is connected to the first branch flow channel 31 through the first manifold flow channel 5 .

[0055] For details, please refer to Figure 7 The first manifold flow channel 5 includes a first main flow channel 51 and a first secondary flow channel 52. The first secondary flow channel 52 is connected to at least two first branch flow channels 31, and the first main flow channel 51 is connected to at least two first secondary flow channels 52. In some other embodiments, the first main flow channel 51 can also be connected to at least one first secondary flow channel 52 and at least one first branch flow channel 31.

[0056] In one embodiment, please refer to Figure 7The first secondary flow channel 52 includes a first stage segment 521 and a first tail segment 522. There are at least two first tail segments 522, and each first tail segment 522 is connected to the first stage segment 521. In some other embodiments, the first secondary flow channel 52 may not be bifurcated, but may be a straight channel.

[0057] In order to facilitate the communication between the second main liquid inlet 2 and the second branch flow channel 32 in each flow channel unit 3, in one embodiment, please refer to Figure 8 The valve integrated module has a second manifold flow channel 6 , which is connected to each second branch flow channel 32 , and the second main liquid inlet 2 is connected to the second branch flow channel 32 through the second manifold flow channel 6 .

[0058] For details, please refer to Figure 8 The second manifold flow channel 6 includes a second main flow channel 61 and a second secondary flow channel 62. The second secondary flow channel 62 is connected to at least two first branch flow channels 31, and the second main flow channel 61 is connected to at least two second secondary flow channels 62. In some other embodiments, the second main flow channel 61 can also be connected to at least one second secondary flow channel 62 and at least one first branch flow channel 31.

[0059] In one embodiment, please refer to Figure 8 The second secondary flow channel 62 includes a second secondary segment 621 and a second end segment 622. There are at least two second end segments 622, and each second end segment 622 is connected to the second secondary segment 621. In some other embodiments, the second secondary flow channel 62 may not be bifurcated, but may be a straight channel.

[0060] Specifically, in one embodiment, the number of flow channel units 3 is four. In one flow channel unit 3, the number of first branch flow channels 31, the number of second branch flow channels 32, the number of first manifold flow channels 5, the number of second manifold flow channels 6, the number of first main flow channels 51, and the number of second main flow channels 61 are all one, and the number of first secondary flow channels 52 and the number of second secondary flow channels 62 are two. In one embodiment, the reversing valve 340 is a two-position three-way solenoid valve.

[0061] In some other embodiments, in addition to using the manifold flow channel, the diameter of the first main liquid inlet 1 and the diameter of the second main liquid inlet 2 can be appropriately increased, and the first branch flow channel 31 and the second branch flow channel 32 can be directly connected to the first main liquid inlet 1 or directly connected to the second main liquid inlet 2.

[0062] In one embodiment, please refer to Figure 7 and Figure 8Each first branch channel 31 and each second branch channel 32 extends along the first direction. Each first branch channel 31 is arranged along the second direction to form a first branch channel group. Each second branch channel 32 is arranged along the second direction to form a second branch channel group. The first branch channel group and the second branch channel group are arranged in layers in the third direction. The first direction, the second direction, and the third direction are the directions in which the three coordinate axes in the three-dimensional coordinate system extend. The layered arrangement of the first and second branch channel groups provides a more compact structure.

[0063] Further, in one embodiment, please refer to Figure 4 and Figure 5 The common flow channel 33 extends along the first direction, and the common flow channels 33 are arranged along the second direction to form a common flow channel group. The first branch flow channel group, the common flow channel group, and the second branch flow channel group are arranged in layers in the third direction. In the same flow channel unit 3, the common flow channel 33 is located between the first branch flow channel 31 and the second branch flow channel 32 in the third direction. The common flow channel 33 is located between the first branch flow channel 31 and the second branch flow channel 32, which facilitates the arrangement of the valve structure 34 and makes the overall structure more compact.

[0064] It should be noted that, in the present application, the first branch channel 31, the common channel 33, and the second branch channel 32 extend in the first direction, which is not limited to the case of extending in a straight line, and at least includes the following cases: for example, in one embodiment, the first branch channel 31, the common channel 33, and the second branch channel 32 are all straight channels; for another example, in one embodiment, the channel extends in the first direction along a broken line or a curve, that is, the overall direction is along the first direction; for another example, in one embodiment, please refer to Figure 5 Although a portion of the common flow channel 33 is inclined, the whole still extends along the first direction; for example, in one embodiment, the first branch flow channel 31, the common flow channel 33 and the second branch flow channel 32 can also be wavy flow channels, but the wavy flow channels make the bends extend along the first direction.

[0065] Furthermore, in one embodiment, please refer to Figure 7 and Figure 8 , the first manifold channel 5 is connected to the first branch channel 31 in each channel unit 3, and the first main liquid inlet 1 is connected to the first branch channel 31 through the first manifold channel 5. The second manifold channel 6 is connected to the second branch channel 32 in each channel unit 3, and the second main liquid inlet 2 is connected to the second branch channel 32 through the second manifold channel 6. The first manifold channel 5 and the second manifold channel 6 are arranged in layers in the first direction. In this way, the layering direction of the first manifold channel 5 and the second manifold channel 6 is perpendicular to the layering direction of the first branch channel group and the second branch channel group, and the overall structure is more compact, which can shorten the length of the channel.

[0066] In some other embodiments, the first branch channel group, the common channel group, and the second branch channel group may be arranged in a staggered arrangement in addition to being arranged in layers. Any feasible arrangement may be adopted based on actual usage requirements. Similarly, the first manifold channel and the second manifold channel may be arranged in any feasible arrangement, such as a cross distribution.

[0067] In one embodiment, when the valve integrated module is used in a sequencer, please refer to Figure 1 The sequencer includes a liquid path system 7 , and the sequencing chip 8 used in the sequencer has at least two sequencing flow channels 81 , each of which has a sequencing flow channel inlet 811 . The sequencing flow channel inlet 811 is connected to the flow channel outlet 331 .

[0068] The fluidic system 7 includes a first rotary valve 71 and a second rotary valve 72. The first rotary valve 71 has a first rotary valve liquid outlet 711 and multiple first rotary valve liquid inlets 712. The first rotary valve liquid inlets 712 are used to communicate with the synthesis sequencing reagent set 91 in the reagent kit 9 ​​and selectively allow the target reagent in the synthesis sequencing reagent set 91 to flow out of the first rotary valve liquid outlet 711. The first rotary valve liquid outlet 711 is connected to the first main liquid inlet 1.

[0069] The second rotary valve 72 has a second rotary valve liquid outlet 721 and a plurality of second rotary valve liquid inlets 722. The second rotary valve liquid inlets 722 are used to communicate with the auxiliary reagent set 92 in the reagent kit 9 ​​and selectively allow the target reagent in the auxiliary reagent set 92 to flow out of the second rotary valve liquid outlet 721. The second rotary valve liquid outlet 721 is connected to the second main liquid inlet 2.

[0070] Specifically, the liquid circuit system 7 further includes a liquid outlet block 10 , a syringe pump 11 , a manifold block 12 and a waste liquid barrel 13 .

[0071] The reagents in the synthetic sequencing reagent group 91 are reagents used in the cycle sequencing process. There are many different types of auxiliary reagents, mainly single-use reagents used in the sequencing preparation stage and after sequencing, such as amplification reagents, cleaning reagents, etc.

[0072] The synthetic sequencing reagents and the auxiliary reagents are separately injected by using the first rotary valve 71 and the second rotary valve 72. The valve structure 34 corresponds to the sequencing flow channel 81 one by one. The valve structure 34 is used to control the sequencing flow channel 81 corresponding to the valve structure 34 to be connected to the first main liquid inlet 1 or the second main liquid inlet 2 separately, so that the sequencing flow channel 81 can select different reagents separately, reducing the waiting time caused by using a common rotary valve pump reagent, and realizing that the flow channel that completes the test first outputs the report first and is taken off the machine for cleaning first, thereby improving the test flexibility.

[0073] The following is a detailed description of the 7-pair valve integration module in conjunction with the hydraulic system:

[0074] Please refer to Figures 1 to 8 A flow channel unit 3 of the valve integrated module corresponds to a sequencing flow channel 81 of the sequencing chip 8, and the flow channel outlet 331 of the common flow channel 33 in the flow channel unit 3 is connected to a sequencing flow channel inlet 811. In this way, the reagent entering the sequencing flow channel 81 is controlled by the corresponding flow channel unit 3.

[0075] The first rotary valve 71 is used to connect the first main liquid inlet 1 with the synthesis sequencing reagent set 91 . The first rotary valve 71 can control the type of reagent entering the first main liquid inlet 1 .

[0076] The second rotary valve 72 is used to connect the second main liquid inlet 2 with the auxiliary reagent group 92. The second rotary valve 72 can control the type of reagent entering the second main liquid inlet 2. The reagents entering the first main liquid inlet 1 and the reagents entering the second main liquid inlet 2 are selected by the valve structure 34 in the flow channel unit 3 to enter the common flow channel 33, thereby supplying different reagents to different sequencing flow channels 81. For example, when one of the sequencing flow channels 81 requires an auxiliary reagent, the flow channel unit 3 corresponding to the sequencing flow channel 81 is connected to the second main liquid inlet 2 and the flow channel outlet 331. For another example, when one of the sequencing flow channels 81 requires a synthetic sequencing reagent, the flow channel unit 3 corresponding to the sequencing flow channel 81 is connected to the first main liquid inlet 1 and the flow channel outlet 331.

[0077] In an embodiment of a fluid circuit system, the fluid circuit system includes a pipeline and a valve integrated module in any of the above embodiments. In an embodiment, the fluid circuit system has the same structure as the fluid circuit system described in any of the above embodiments, and no further description is given.

[0078] In one embodiment of a sequencing instrument, the sequencing instrument includes a fluidic system and a sequencing chip carrier platform, wherein the fluidic system includes the valve integrated module described in any of the above embodiments. In one embodiment, the structure of the sequencing instrument is the same as that of the sequencing instrument described in any of the above embodiments, and no further description is given.

[0079] The above examples are used to illustrate the present invention, which are only used to help understand the present invention and are not intended to limit the present invention. Those skilled in the art can make several simple deductions, modifications or substitutions based on the concept of the present invention.

Claims

1. A valve integrated module, characterized in that: The valve integrated module has a first main liquid inlet and a second main liquid inlet, and the valve integrated module includes at least two flow channel units; the flow channel unit includes: a first branch flow channel, the first branch flow channel being in communication with the first main liquid inlet; a second branch flow channel, the second branch flow channel being in communication with the second main liquid inlet; A common flow channel, wherein the common flow channel has a flow channel outlet; And a valve structure, the valve structure has a first valve inlet, a second valve inlet and a valve outlet, the valve outlet is connected to the common flow channel, the first valve inlet is connected to the first branch flow channel, and the second valve inlet is connected to the second branch flow channel; the valve structure includes a valve core, the valve core has a first state and a second state; when the valve core is in the first state, the first branch flow channel is connected to the common flow channel and the second branch flow channel is disconnected from the common flow channel; when the valve structure is in the second state, the second branch flow channel is connected to the common flow channel and the first branch flow channel is disconnected from the common flow channel.

2. The valve integrated module according to claim 1, characterized in that: The valve integrated module has a first manifold flow channel, the first manifold flow channel is connected to each of the first branch flow channels, and the first main liquid inlet is connected to each of the first branch flow channels through the first manifold flow channel; and / or the valve integrated module has a second manifold flow channel, the second manifold flow channel is connected to each of the second branch flow channels, and the second main liquid inlet is connected to each of the second branch flow channels through the second manifold flow channel.

3. The valve integrated module according to claim 2, characterized in that: The first manifold flow channel includes a main flow channel and a secondary flow channel, the secondary flow channel is connected to at least two of the first branch flow channels; the main flow channel is connected to at least two of the secondary flow channels, or the main flow channel is connected to at least one of the secondary flow channels and at least one of the first branch flow channels.

4. The valve integrated module according to claim 1, wherein: Each of the first branch flow channels and each of the second branch flow channels extends along the first direction, each of the first branch flow channels is arranged along the second direction to form a first branch flow channel group, and each of the second branch flow channels is arranged along the second direction to form a second branch flow channel group. The first branch flow channel group and the second branch flow channel group are arranged in layers in a third direction, and the first direction, the second direction and the third direction are respectively the extension directions of the three coordinate axes in the three-dimensional coordinate system.

5. The valve integrated module according to claim 4, characterized in that: The common flow channel extends along the first direction, and each common flow channel is arranged along the second direction to form a common flow channel group, and the first branch flow channel group, the common flow channel group and the second branch flow channel group are arranged in layers in the third direction; in the same flow channel unit: the common flow channel is between the first branch flow channel and the second branch flow channel in the third direction.

6. The valve integrated module according to claim 4, characterized in that: The valve integrated module has a first manifold flow channel, which is connected to the first branch flow channel in each of the flow channel units, and the first main liquid inlet is connected to each of the first branch flow channels through the first manifold flow channel; the valve integrated module has a second manifold flow channel, which is connected to the second branch flow channel in each of the flow channel units, and the second main liquid inlet is connected to each of the second branch flow channels through the second manifold flow channel; the first manifold flow channel and the second manifold flow channel are arranged in layers in the first direction.

7. The valve integrated module according to any one of claims 1 to 6, characterized in that: The valve integrated module includes a valve block and a reversing valve fixed on the valve block, the reversing valve forms the valve structure, the valve block has a reversing valve mounting surface for mounting the reversing valve, the outlet of the first branch flow channel, the outlet of the second branch flow channel and the inlet of the common flow channel are all located on the reversing valve mounting surface.

8. The valve integrated module according to claim 7, characterized in that: The valve block has a liquid outlet side surface opposite to the installation surface of the reversing valve, and the flow channel outlet is arranged on the liquid outlet side surface.

9. The valve integrated module according to claim 8, characterized in that: The valve block further has a liquid inlet side surface, the liquid inlet side surface connects the liquid outlet side surface and the reversing valve mounting surface, and the first main liquid inlet and the second main liquid inlet are located on the liquid inlet side surface.

10. The valve integrated module according to any one of claims 1 to 6, characterized in that: Each of the valve cores is an electrically controlled valve core that can be independently controlled.

11. A fluid system, characterized in that: The invention comprises a pipeline and a valve integrated module as described in any one of claims 1 to 10.

12. A gene sequencer, characterized in that: It comprises a liquid circuit system and a sequencing chip carrying platform, characterized in that the liquid circuit system comprises pipelines and a valve integrated module as described in any one of claims 1-10.

13. The gene sequencer according to claim 12, characterized in that: The flow channel outlet is used to communicate with the sequencing flow channel inlet on the sequencing chip used by the gene sequencer; The liquid circuit system includes a first rotary valve and a second rotary valve, wherein the first rotary valve has a first rotary valve liquid outlet and a plurality of first rotary valve liquid inlets, wherein the first rotary valve liquid inlet is used to communicate with the synthesis sequencing reagent set in the reagent kit and selectively allow the target reagent in the synthesis sequencing reagent set to flow out from the first rotary valve liquid outlet; the first rotary valve liquid outlet is communicated with the first main liquid inlet; The second rotary valve has a second rotary valve liquid outlet and multiple second rotary valve liquid inlets. The second rotary valve liquid inlet is used to communicate with the auxiliary reagent group in the reagent kit and select to allow the target reagent in the auxiliary reagent group to flow out from the second rotary valve liquid outlet; the second rotary valve liquid outlet is connected to the second main liquid inlet.

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