Composition with tooth repairing and whitening effects and application thereof

Abstract

The invention relates to a composition with tooth repairing and whitening effects and application thereof. The composition is prepared from paeonol, honokiol, allantoin and a crocus sativus extract. The invention creatively discovers that paeonol, honokiol, allantoin and crocus sativus extract have a remarkable synergistic effect in the aspects of repairing and whitening the teeth. The four components are compounded for use, so that dental plaque bacteria can be remarkably reduced, gingival inflammation can be relieved, and meanwhile, the tooth whitening effect can be improved.

Description

Technical Field

[0001] The present invention belongs to the technical field of daily necessities, and in particular relates to a composition with teeth repairing and whitening effects and an application thereof. Background Art

[0002] Tooth sensitivity is a common oral condition, typically manifesting as sharp pain in response to cold, hot, sour, or sweet stimuli. The main causes of tooth sensitivity include individual factors such as gum recession exposing dentin, tooth wear, bruxism, gum disease, tooth damage, and plaque buildup at the root of the tooth. Other external factors include acidic foods, teeth whitening products, long-term use of acidic mouthwashes, and dental treatments.

[0003] As consumers' health awareness continues to rise, they demand that toothpaste not only clean and whiten teeth, but also provide auxiliary functions to prevent or treat oral diseases. To better prevent and treat gingivitis and reduce gum bleeding, tranexamic acid is commonly added to toothpaste. However, the addition of tranexamic acid can cause gum antibodies, and tranexamic acid is a prescription drug, which inevitably increases toothpaste production costs and the risks of raw material use.

[0004] With the increasing demand for oral care and the pursuit of natural, safe and effective oral care products, developing a toothpaste product that can quickly and effectively protect dental health, relieve tooth sensitivity, and improve whitening effects has become one of the technical problems that need to be solved urgently. Summary of the Invention

[0005] In view of the shortcomings of the prior art, the present invention aims to provide a composition with tooth repair and whitening effects and its application. The composition can significantly reduce dental plaque, relieve gingivitis, and improve the tooth whitening effect.

[0006] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0007] In a first aspect, the present invention provides a composition having the effects of tooth repairing and whitening, wherein the composition comprises paeonol, honokiol, allantoin and saffron extract.

[0008] Paeonol has strong antibacterial activity against common pathogens and microorganisms such as Escherichia coli, Staphylococcus aureus, and Staphylococcus epidermidis. It can prevent and treat infectious diseases induced by various pathogenic microorganisms, improve the phagocytosis of these pathogens by peripheral blood neutrophils, and inhibit or reduce the amount of dental plaque. Honokiol is mainly derived from the bark of Magnolia officinalis or Magnolia officinalis, both of the Magnoliaceae family. It has broad-spectrum antibacterial activity, can inhibit oral pathogens, and has anti-inflammatory effects. Allantoin is a heterocyclic compound found in plants of the Boraginaceae family and in mammalian urine. It is highly safe, mild and non-irritating to the skin and mucous membranes, can accelerate wound healing, and regulate the oral environment. Saffron extract is derived from the Iridaceae family plant Crocus sativus. The main active ingredients are crocin and crocetin, and it has strong antioxidant and anti-inflammatory properties.

[0009] The present invention innovatively discovered that paeonol, honokiol, allantoin, and crocus sativus extract have a significant synergistic effect in terms of tooth repair and whitening. The combination of these four components can significantly reduce dental plaque, alleviate gingivitis, and enhance teeth whitening.

[0010] Preferably, the composition comprises, by mass, 1-5 parts of paeonol, 1-5 parts of honokiol, 5-10 parts of allantoin and 0.5-1 part of saffron extract.

[0011] Among them, the specific point values ​​in 1-5 parts can be selected from 1 part, 2 parts, 3 parts, 4 parts, 5 parts, etc., the specific point values ​​in 5-10 parts can be selected from 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc., and the specific point values ​​in 0.5-1 parts can be selected from 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts, 0.9 parts, 1 part, etc.

[0012] Preferably, the saffron extract is prepared by a preparation method comprising the following steps:

[0013] (1) Mixing the saffron raw material with an ethanol aqueous solution, performing percolation extraction, and collecting the extract to obtain a saffron crude extract.

[0014] (2) The crude saffron extract is mixed with a flocculant for flocculation, and the supernatant is collected by centrifugation to obtain a saffron extract.

[0015] The invention performs percolation extraction on saffron raw materials and performs flocculation treatment on the crude extract, thereby further increasing the content of effective components in the extract and improving the teeth repairing and whitening effects of the saffron extract.

[0016] Preferably, the saffron raw material in step (1) includes saffron stigma and saffron petals.

[0017] The present invention creatively discovered that, in the process of preparing saffron raw materials, saffron stigmas are mixed with saffron petals, and together they are used as raw materials for preparing saffron extract, so that the repairing and whitening effects of the prepared saffron extract can be more fully exerted.

[0018] Preferably, the mass ratio of the saffron stigma to the saffron petals is 1:(0.1-0.5), for example, it can be 1:0.1, 1:0.2, 1:0.3, 1:0.4, 1:0.5, etc.

[0019] Preferably, the percolation extraction method in step (1) is segmented percolation extraction.

[0020] The method of the segmented percolation extraction comprises:

[0021] (I) Mixing the saffron raw material with an ethanol aqueous solution having a volume fraction of 80-90%, performing percolation extraction, and obtaining an extract I.

[0022] (II) Mixing the saffron raw material with 50-60% by volume of an ethanol aqueous solution, performing percolation extraction, and obtaining an extract II.

[0023] The extracts I and II were combined to obtain a crude saffron extract.

[0024] The present invention adopts ethanol aqueous solutions of different concentrations to perform segmented percolation on the saffron raw material, which can fully extract the effective ingredients in the saffron and further enhance the repairing and whitening effects of the tomato flower extract.

[0025] Preferably, the material-liquid ratio of the saffron raw material in step (I) to the ethanol aqueous solution with a volume fraction of 80-90% is 1:(10-30) g / mL.

[0026] Preferably, the temperature of the percolation extraction in step (I) is 20-40° C., and the flow rate of the percolation extraction is 2-4 mL / min.

[0027] Preferably, the material-liquid ratio of the saffron raw material to the ethanol aqueous solution with a volume fraction of 50-60% in step (II) is 1:(10-30) g / mL.

[0028] Preferably, the temperature of the percolation extraction in step (II) is 20-40° C., and the flow rate of the percolation extraction is 2-4 mL / min.

[0029] Among them, the specific point values ​​in 80-90% can be selected from 80%, 82%, 84%, 86%, 88%, 90%, etc., and the specific point values ​​in 1: (10-30) g / mL can be selected from 1: 10 g / mL, 1: 15 g / mL, 1: 20 g / mL,

[0030] 1:25g / mL, 1:30g / mL, etc.; the specific point values ​​in 20-40℃ can be selected from 20℃, 25℃, 30℃, 35℃, 40℃, etc.; the specific point values ​​in 2-4mL / min can be selected from 2mL / min, 2.5mL / min, 3mL / min, 3.5mL / min, 4mL / min, etc.; the specific point values ​​in 50-60% can be selected from 50%, 52%, 54%, 56%, 58%, 60%, etc.

[0031] Preferably, the flocculant in step (2) comprises chitosan, sodium carboxymethyl starch and gelatin.

[0032] During the preparation of saffron extract, chitosan, sodium carboxymethyl starch and gelatin are used as flocculants to flocculate the saffron extract, which can further enhance the enrichment effect of the effective ingredients, improve the teeth repair and whitening effects of the extract, and improve the stability of the product.

[0033] Preferably, the mass ratio of chitosan, sodium carboxymethyl starch and gelatin is 1:(1-5):(0.1-1).

[0034] Among them, the specific point values ​​in 1-5 can be selected from 1, 2, 3, 4, 5, etc., and the specific point values ​​in 0.1-1 can be selected from 0.1, 0.3, 0.5, 0.7, 0.9, 1, etc.

[0035] Preferably, the mass ratio of the crude saffron extract to the flocculant is 100:(0.1-2), for example, it can be 100:0.1, 100:0.5, 100:1, 100:1.5, 100:2, etc.

[0036] In a second aspect, the present invention provides a toothpaste with tooth repairing and whitening effects, the toothpaste comprising the composition with tooth repairing and whitening effects described in the first aspect, a surfactant, an abrasive, an anticaries agent, a thickener, a chelating agent, a moisturizer and a solvent.

[0037] Preferably, the toothpaste comprises, by mass, 1-5 parts of the composition with tooth repairing and whitening effects described in the first aspect, 2-10 parts of a surfactant, 10-20 parts of an abrasive, 0.1-1 part of an anticaries agent, 1-5 parts of a thickener, 0.1-1 part of a chelating agent, 20-40 parts of a moisturizer, and 20-65 parts of a solvent.

[0038] Among them, the specific point values ​​in 1-5 portions can be selected from 1 portion, 2 portions, 3 portions, 4 portions, 5 portions, etc., the specific point values ​​in 2-10 portions can be selected from 2 portions, 4 portions, 6 portions, 8 portions, 10 portions, etc., the specific point values ​​in 10-20 portions can be selected from 10 portions, 12 portions, 14 portions, 16 portions, 18 portions, 20 portions, etc., the specific point values ​​in 0.1-1 portions can be selected from 0.1 portions, 0.3 portions, 0.5 portions, 0.7 portions, 0.9 portions, 1 portion, etc., the specific point values ​​in 20-40 portions can be selected from 20 portions, 25 portions, 30 portions, 35 portions, 40 portions, etc., and the specific point values ​​in 20-65 portions can be selected from 20 portions, 30 portions, 40 portions, 50 portions, 60 portions, 65 portions, etc.

[0039] Preferably, the abrasive comprises hydrated silica.

[0040] Preferably, the surfactant comprises cocamidopropyl betaine.

[0041] Preferably, the anticaries agent comprises sodium fluoride.

[0042] Preferably, the thickening agent comprises cellulose gum and / or carbomer.

[0043] Preferably, the chelating agent comprises tetrasodium pyrophosphate.

[0044] Preferably, the moisturizing agent comprises any one of sorbitol, glycerin or polyethylene glycol, or a combination of at least two of them.

[0045] Preferably, the solvent comprises water.

[0046] In a third aspect, the present invention provides a method for preparing the toothpaste having the functions of tooth repairing, whitening and protecting teeth as described in the second aspect, the preparation method comprising the following steps:

[0047] (i) mixing paeonol, honokiol and a surfactant to obtain a first mixture; and mixing allantoin, saffron extract, an abrasive, an anticaries agent, a thickener, a chelating agent, a moisturizing agent and a solvent to obtain a second mixture.

[0048] (ii) mixing the first mixture and the second mixture to obtain the toothpaste having the functions of tooth repairing and whitening.

[0049] Preferably, the temperature of the mixing and stirring before obtaining the first mixture in step (i) is 20-40° C., and the time of the mixing and stirring is 5-10 min.

[0050] Preferably, the mixing and stirring temperature before obtaining the second mixture in step (i) is 40-70° C., and the mixing and stirring time is 5-10 min.

[0051] Preferably, the mixing and stirring temperature in step (ii) is 20-40° C., and the mixing and stirring time is 10-20 min.

[0052] Among them, the specific point values ​​in 20-40℃ can be selected from 20℃, 25℃, 30℃, 35℃, 40℃, etc., the specific point values ​​in 40-70℃ can be selected from 40℃, 45℃, 50℃, 55℃, 60℃, 65℃, 70℃, etc., the specific point values ​​in 5-10min can be selected from 5min, 6min, 7min, 8min, 9min, 10min, etc., and the specific point values ​​in 10-20min can be selected from 10min, 12min, 14min, 16min, 18min, 20min, etc.

[0053] The numerical range described in the present invention includes not only the point values ​​listed above, but also any point values ​​between the above numerical ranges that are not listed. Due to space limitations and for the sake of simplicity, the present invention no longer exhaustively lists the specific point values ​​included in the range.

[0054] Compared with the prior art, the present invention has the following beneficial effects:

[0055] The present invention creatively develops a composition with both tooth repairing and whitening effects, which comprises paeonol, honokiol, allantoin and saffron extract; and experiments have shown that the above components have significant synergistic effects in tooth repairing and whitening.

[0056] Furthermore, during an investigation of saffron raw materials, it was discovered that mixing saffron stigmas with saffron petals as raw materials for preparing saffron extract can further enhance the repair and whitening effects of the resulting saffron extract. Furthermore, co-extraction of saffron stigmas and saffron petals exhibits superior repair and whitening effects compared to extracting saffron stigmas and saffron petals separately and then mixing them.

[0057] Furthermore, in the preparation process of saffron extract, 80-90% ethanol aqueous solution and 50-60% ethanol aqueous solution are used successively to perform segmented percolation on the saffron raw material, which can fully extract the effective ingredients in saffron and further enhance the repair and whitening effects of tomato flower extract.

[0058] Furthermore, in the preparation process of saffron extract, the saffron extract is flocculated using a combination of chitosan, sodium carboxymethyl starch and gelatin as a flocculant, which can further enhance the enrichment effect of the effective ingredients, improve the teeth repair and whitening effects of the extract, and at the same time improve the stability of the product. DETAILED DESCRIPTION

[0059] In order to further illustrate the technical means and effects adopted by the present invention, the technical solutions of the present invention are further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0060] The sources of the components / raw materials in the following embodiment are shown in Table 1.

[0061] Table 1

[0062]

[0063]

[0064] Preparation Example 1

[0065] This preparation example provides a saffron extract, and the preparation method is as follows:

[0066] (1) The stigma and petals of crocus were crushed separately, and the mixture was mixed in a mass ratio of stigma to petals of 1:0.3 to obtain a crocus raw material mixture.

[0067] The saffron raw material mixture was mixed with 85% (v / v) ethanol aqueous solution at a solid-liquid ratio of 1:20 g / mL, and percolation extraction was performed (temperature at 30°C, flow rate at 3 mL / min) to obtain extract I; then mixed with 55% (v / v) ethanol aqueous solution at a solid-liquid ratio of 1:20 g / mL, and percolation extraction was performed (temperature at 30°C, flow rate at 3 mL / min) to obtain extract II; extract I and extract II were combined to obtain a crude saffron extract.

[0068] (2) A flocculant (composed of 1 part chitosan, 3 parts sodium carboxymethyl starch and 0.5 parts gelatin in parts by weight) with a mass of 1% of the mass of the crude saffron extract was heated and dissolved with water, and then cooled and mixed with the crude saffron extract. The mixture was stirred at 30°C for 1 hour for flocculation treatment, centrifuged at 2000 rpm for 30 minutes, and the supernatant was collected. The supernatant was concentrated under reduced pressure to 15wt%, sterilized, and freeze-dried to obtain a saffron extract.

[0069] Preparation Example 2

[0070] This preparation example provides a saffron extract, and the preparation method is as follows:

[0071] (1) The stigma and petals of crocus were crushed separately, and the mixture was mixed in a mass ratio of stigma to petals of 1:0.1 to obtain a crocus raw material mixture.

[0072] The saffron raw material mixture was mixed with 80% (v / v) ethanol aqueous solution at a solid-liquid ratio of 1:10 g / mL, and percolation extraction was performed (temperature at 20°C, flow rate at 2 mL / min) to obtain extract I; then mixed with 50% (v / v) ethanol aqueous solution at a solid-liquid ratio of 1:10 g / mL, and percolation extraction was performed (temperature at 20°C, flow rate at 2 mL / min) to obtain extract II; extract I and extract II were combined to obtain a crude saffron extract.

[0073] (2) A flocculant (composed of 1 part chitosan, 1 part sodium carboxymethyl starch and 0.1 part gelatin in parts by weight) with a mass of 0.1% of the mass of the crude saffron extract was heated and dissolved with water, and then cooled and mixed with the crude saffron extract. The mixture was stirred at 30°C for 1 hour for flocculation treatment, centrifuged at 2000 rpm for 50 minutes, and the supernatant was collected. The supernatant was concentrated under reduced pressure to 15wt%, sterilized, and freeze-dried to obtain a saffron extract.

[0074] Preparation Example 3

[0075] This preparation example provides a saffron extract, and the preparation method is as follows:

[0076] (1) The stigma and petals of crocus were crushed separately, and the mixture was mixed in a mass ratio of stigma to petals of 1:0.5 to obtain a crocus raw material mixture.

[0077] The saffron raw material mixture was mixed with a 90% (v / v) ethanol aqueous solution at a solid-liquid ratio of 1:30 g / mL, and percolation extraction was performed (temperature of 40°C, flow rate of 4 mL / min) to obtain extract I; then mixed with a 60% (v / v) ethanol aqueous solution at a solid-liquid ratio of 1:30 g / mL, and percolation extraction was performed (temperature of 40°C, flow rate of 4 mL / min) to obtain extract II; extract I and extract II were combined to obtain a crude saffron extract.

[0078] (2) A flocculant (composed of 1 part chitosan, 5 parts sodium carboxymethyl starch and 1 part gelatin in parts by weight) with a mass of 2% of the mass of the crude saffron extract was heated and dissolved with water, cooled, and then mixed with the crude saffron extract. The mixture was stirred at 30°C for 1 hour for flocculation treatment, centrifuged at 2000 rpm for 40 minutes, and the supernatant was collected. The supernatant was concentrated under reduced pressure to 15wt%, sterilized, and freeze-dried to obtain a saffron extract.

[0079] Preparation Example 4

[0080] This preparation example provides a crocus extract, which differs from Preparation Example 1 only in that, in step (1), the total mass of the crocus raw material mixture is maintained constant, and only the crocus stigma is used as the raw material for extraction, i.e., the crocus stigma is crushed to obtain stigma powder; and the crocus raw material mixture (stigma powder) is mixed with an ethanol aqueous solution for percolation extraction. The remaining steps remain the same as in Preparation Example 1.

[0081] Preparation Example 5

[0082] This Preparation Example provides a crocus flower extract, which differs from Preparation Example 1 only in that, in step (1), the total mass of the crocus flower raw material mixture is maintained constant, and only crocus flower petals are used as raw materials for extraction, i.e., the crocus flower petals are crushed to obtain petal powder; and the crocus flower raw material mixture (petal powder) is mixed with an ethanol aqueous solution for percolation extraction. The remaining steps remain the same as in Preparation Example 1.

[0083] Preparation Example 6

[0084] This Preparation Example provides a crocus extract, which differs from Preparation Example 1 only in that the crocus stigmas and crocus petals are extracted separately. Specifically, the tomato flower extract obtained in Preparation Example 3 (the crocus raw material is solely the tomato flower stigmas) and the tomato flower extract obtained in Preparation Example 4 (the crocus raw material is solely the tomato flower petals) are mixed in a mass ratio of 1:0.3 to provide the crocus extract provided in this Example.

[0085] Preparation Example 7

[0086] This preparation example provides a crocus flower extract, which differs from Preparation Example 1 only in that, in step (1), the volume fraction of the ethanol aqueous solution is adjusted to 75% (v / v) before obtaining Extract Solution I; and the volume fraction of the ethanol aqueous solution is adjusted to 45% (v / v) before obtaining Extract Solution II. The remaining steps remain the same as in Preparation Example 1.

[0087] Preparation Example 8

[0088] This preparation example provides a crocus flower extract, which differs from Preparation Example 1 only in that, in step (1), the volume fraction of the ethanol aqueous solution is adjusted to 95% (v / v) before obtaining Extract I; and the volume fraction of the ethanol aqueous solution is adjusted to 65% (v / v) before obtaining Extract II. The remaining steps remain the same as in Preparation Example 1.

[0089] Preparation Example 9

[0090] This preparation example provides a saffron extract, which differs from Preparation Example 1 only in that, in step (2), the total amount of flocculant added remains unchanged and the flocculant is adjusted to consist of 1 part of chitosan and 3 parts of sodium carboxymethyl starch in parts by weight, and the remaining steps remain the same as Preparation Example 1.

[0091] Preparation Example 10

[0092] This preparation example provides a saffron extract, which differs from Preparation Example 1 only in that, in step (2), the total amount of flocculant added remains unchanged and the flocculant is adjusted to: 1 part chitosan and 0.5 part gelatin in parts by weight, and the remaining steps remain the same as Preparation Example 1.

[0093] Preparation Example 11

[0094] This preparation example provides a saffron extract, which differs from Preparation Example 1 only in that, in step (2), the total amount of flocculant added remains unchanged and the flocculant is adjusted to consist of 3 parts of sodium carboxymethyl starch and 0.5 parts of gelatin in parts by weight. The remaining steps remain the same as Preparation Example 1.

[0095] Example 1

[0096] This embodiment provides a composition, which comprises, by mass, 3 parts of paeonol, 3 parts of honokiol, 7 parts of allantoin, and 0.7 parts of the saffron extract obtained in Preparation Example 1.

[0097] Example 2

[0098] This embodiment provides a composition, which comprises, by mass, 1 part of paeonol, 5 parts of honokiol, 5 parts of allantoin, and 0.5 parts of the saffron extract obtained in Preparation Example 2.

[0099] Example 3

[0100] This embodiment provides a composition, which comprises, by mass, 5 parts of paeonol, 1 part of honokiol, 10 parts of allantoin and 1 part of the saffron extract obtained in Preparation Example 3.

[0101] Example 4-Example 11

[0102] Example 4-Example 11 each provide a composition, which differs from Example 1 only in that the saffron extract obtained in Preparation Example 1 is replaced by the saffron extract obtained in Preparation Examples 4-Preparation Examples 11 of equal mass, respectively.

[0103] Comparative Example 1

[0104] This comparative example provides a composition, which comprises, by mass, 3 parts of honokiol, 7 parts of allantoin and 0.7 parts of the saffron extract obtained in Preparation Example 1.

[0105] Comparative Example 2

[0106] This comparative example provides a composition, which comprises, by mass, 3 parts of paeonol, 7 parts of allantoin and 0.7 parts of the saffron extract obtained in Preparation Example 1.

[0107] Comparative Example 3

[0108] This comparative example provides a composition, which comprises, by mass, 3 parts of paeonol, 3 parts of honokiol and 0.7 parts of the saffron extract obtained in Preparation Example 1.

[0109] Comparative Example 4

[0110] This comparative example provides a composition, which comprises, by weight, 3 parts of paeonol, 3 parts of honokiol and 7 parts of allantoin.

[0111] Application Example 1

[0112] This application example provides a toothpaste, which includes 5 parts of the composition of Example 1, 2 parts of a surfactant (cocamidopropyl betaine), 15 parts of an abrasive (hydrated silica), 0.5 parts of an anticaries agent (sodium fluoride), 3 parts of a thickener (cellulose gum and carbomer in a mass ratio of 5:2), 0.5 parts of a chelating agent (tetrasodium pyrophosphate), 30 parts of a humectant (sorbitol, glycerol and polyethylene glycol-8 in a mass ratio of 1:1:0.2), and the balance is a solvent (water).

[0113] The preparation process is:

[0114] Paeonol, honokiol and a surfactant were mixed and stirred at 30° C. for 8 minutes to obtain a first mixture; allantoin, saffron extract, an abrasive, an anticaries agent, a thickener, a chelating agent, a moisturizer and a solvent were mixed and stirred at 50° C. for 8 minutes to obtain a second mixture;

[0115] The first mixture and the second mixture were mixed and stirred at 30° C. for 15 minutes to obtain toothpaste.

[0116] Application Example 2

[0117] This application example provides a toothpaste, which includes 3 parts of the composition of Example 2, 3 parts of a surfactant (cocamidopropyl betaine), 20 parts of an abrasive (hydrated silica), 1 part of an anticaries agent (sodium fluoride), 1 part of a thickener (cellulose gum and carbomer in a mass ratio of 1:1), 1 part of a chelating agent (tetrasodium pyrophosphate), 20 parts of a humectant (sorbitol, glycerol and polyethylene glycol-8 in a mass ratio of 2:1:0.5), and the balance is a solvent (water).

[0118] The preparation process is:

[0119] Paeonol, honokiol and a surfactant were mixed and stirred at 20° C. for 10 minutes to obtain a first mixture; allantoin, saffron extract, an abrasive, an anticaries agent, a thickener, a chelating agent, a moisturizer and a solvent were mixed and stirred at 40° C. for 10 minutes to obtain a second mixture;

[0120] The first mixture and the second mixture were mixed and stirred at 20° C. for 20 min to obtain toothpaste.

[0121] Application Example 3

[0122] This application example provides a toothpaste, which includes 1 part of the composition of Example 3, 5 parts of a surfactant (cocamidopropyl betaine), 10 parts of an abrasive (hydrated silica), 0.1 parts of an anticaries agent (sodium fluoride), 5 parts of a thickener (cellulose gum and carbomer in a mass ratio of 0.5:1), 0.1 parts of a chelating agent (tetrasodium pyrophosphate), 40 parts of a humectant (sorbitol, glycerol and polyethylene glycol-8 in a mass ratio of 1:2:1), and the balance is a solvent (water).

[0123] The preparation process is:

[0124] Paeonol, honokiol and a surfactant were mixed and stirred at 40° C. for 5 minutes to obtain a first mixture; allantoin, saffron extract, an abrasive, an anticaries agent, a thickener, a chelating agent, a moisturizer and a solvent were mixed and stirred at 70° C. for 5 minutes to obtain a second mixture;

[0125] The first mixture and the second mixture were mixed and stirred at 40° C. for 10 minutes to obtain toothpaste.

[0126] Application Example 4-Application Example 11

[0127] Application Examples 4 to 11 each provide a toothpaste, each differing from Application Example 1 only in that the composition of Example 1 in Application Example 1 is replaced by the compositions of Examples 4 to 11. The remaining formulas and preparation processes remain the same as those of Application Example 1.

[0128] Comparative Application Example 1-Comparative Application Example 4

[0129] Comparative Application Examples 1 to 4 each provide a toothpaste, each differing from Application Example 1 only in that the composition of Example 1 in Application Example 1 is replaced by the compositions of Comparative Examples 1 to 4, respectively. The remaining formulas and preparation processes remain the same as those of Application Example 1.

[0130] Test Example 1

[0131] Dental plaque inhibition effect test:

[0132] (1) Test sample: toothpaste obtained from each application example and comparative application example.

[0133] (2) Test method:

[0134] (2.1) 150 subjects aged 18-65 years (without severe oral or chronic diseases, severe periodontal disease, or dental calculus) were recruited and divided equally into 15 groups, with 10 subjects in each group. They were instructed to brush their teeth once daily, morning and evening, using a standard soft-bristled toothbrush and toothpaste. The amount of toothpaste used was approximately 1 / 2-2 / 3 full (approximately 1 g) of toothpaste, using the standard modified Bass technique for 3 minutes each time. After using the toothpaste for one month, dental plaque was examined before and after use.

[0135] (2.2) Dental plaque test method: The subject first rinsed his mouth with clean water, and then stained the subject's marked teeth (i.e., upper right, lower left 6 and lower right, upper left 1, 4) with a cotton swab containing plaque stain. After leaving it for 1 minute, rinsed his mouth again and examined the four areas of the marked teeth, i.e., the central buccal surface, the distal buccal surface, the mesial buccal surface, and the lingual surface.

[0136] (2.3) Dental Plaque Assessment: Plaque accumulation is graded using the Plaque Index (PLI), with a scale of 0-3 evaluating the degree of plaque accumulation (see Table 2). Four areas of the marked teeth were examined and recorded separately. The sum of the scores for each tooth divided by 4 yielded the tooth's score. The sum of the scores for each tooth, divided by the number of teeth, yielded the individual score.

[0137] Table 2

[0138]

[0139] (3) Test results:

[0140] Calculate the dental plaque change rate of each sample subject.

[0141] Dental plaque change rate (%) = (individual plaque index before the test - individual plaque index after 1 month of use) / individual plaque index before the test × 100%.

[0142] The test results of each sample are shown in Table 3.

[0143] Table 3

[0144]

[0145]

[0146] Test Example 2

[0147] Gum problem relief effect test:

[0148] (1) Test sample: toothpaste obtained from each application example and comparative application example.

[0149] (2) Test method:

[0150] (2.1) The same group of subjects from Test Example 1 were divided equally into 15 groups, with 10 subjects in each group. They were instructed to brush their teeth once daily, morning and evening, using the provided soft-bristled toothbrush and toothpaste. The toothpaste should fill 1 / 2-2 / 3 of the toothbrush head (approximately 1 gram) using the standard modified Bass technique for 3 minutes each time. The toothpaste was used continuously for 1 month. Gum bleeding was examined before and after 2 months of use.

[0151] (2.2) Gum Bleeding Test Method: Gum inflammation is graded using the Gum Index (GI) on a scale of 0-3 (see Table 4 for the GI score table). Four areas of the marked teeth are examined and recorded separately. The sum of the scores for each tooth divided by 4 gives the tooth's score. The sum of the scores for each tooth divided by the number of teeth gives the individual score.

[0152] Table 4

[0153]

[0154]

[0155] (3) Test results:

[0156] Calculate the dental plaque change rate of each sample subject.

[0157] Gingival index change rate (%) = (individual gingival index before the test - individual gingival index after 2 months of use) / individual gingival index before the test × 100%.

[0158] The test results of each sample are shown in Table 5.

[0159] Table 5

[0160]

[0161]

[0162] Test Example 3

[0163] Whitening effect test:

[0164] (1) Test sample: toothpaste obtained from each application example and comparative application example.

[0165] (2) Test method:

[0166] (2.1) Preparation of tobacco, tea, and coffee stain model: Place No. 20 L390 Bayer simulated tooth in a sealed container and fumigate with strong smoke for 30 minutes to color it. Remove and naturally dry it. Then soak it in strong tea (30g tea + 500mL boiling water for 10 minutes) for 48 hours, remove and naturally dry it. Then soak it in strong coffee liquid (50%) for 48 hours to make a tobacco, tea, and coffee stain model of simulated tooth.

[0167] (2.2) Test samples:

[0168] The above-mentioned simulated tooth tobacco, tea, and coffee stain models were divided into 15 groups, with 5 simulated tooth specimens in each group. 10g of toothpaste from each of the 15 groups (the toothpaste obtained from each application example and the comparative application example) was taken and placed in a test cup. 30mL of water was added and mixed evenly. The colored simulated tooth specimens were immersed in the toothpaste slurry for 24 hours of immersion. After the time was up, the specimens were taken out and the toothpaste on the surface was gently rinsed with running water. The specimens were naturally dried for later use. The L* values ​​of the Bayer simulated teeth before staining, after staining, and 24 hours after bleaching were measured using a Minolta CR-33IC colorimeter from Japan. The L* change rate after staining and bleaching was calculated for each group.

[0169] L* change rate (%) = (L* value 24 hours after bleaching - L* value after dyeing) / L* value after dyeing × 100%.

[0170] The test results of each sample are shown in Table 6.

[0171] Table 6

[0172] Samples to be tested L* change rate Application Example 1 17％ Application Example 2 14％ Application Example 3 15％ Application Example 4 9％ Application Example 5 8％ Application Example 6 12％ Application Example 7 7％ Application Example 8 10％ Application Example 9 6％ Application Example 10 11％ Application Example 11 5％ Comparative Application Example 1 2％ Comparative Application Example 2 5％ Comparative Application Example 3 4％ Comparative Application Example 4 3％

[0173] It can be seen from the data in Tables 3 to 6 that the composition of the present invention can significantly reduce dental plaque, prevent and treat gingivitis, and further enhance the whitening effect of teeth.

[0174] From the comparison of the data of Application Example 1 and Comparative Application Examples 1-4, it can be seen that paeonol, honokiol, allantoin and saffron extract have significant synergistic effects in tooth repair and whitening.

[0175] From the data comparison of Application Example 1 and Application Examples 4-5, it can be seen that in the preparation process of saffron extract, saffron stigmas are mixed with saffron petals and used together as raw materials for the preparation of saffron extract, which can more fully exert the repair and whitening effects of the prepared saffron extract.

[0176] From the comparison of the data of Application Example 1 and Application Example 6, it can be seen that in the preparation process of saffron extract, saffron stigma and saffron petals are co-extracted. Compared with the extraction of saffron stigma and saffron petals separately and then mixing the extracts, the prepared saffron extract has better repairing and whitening effects.

[0177] From the comparison of the data of Application Example 1 and Application Examples 7-8, it can be seen that in the preparation process of saffron extract, 80-90% ethanol aqueous solution and 50-60% ethanol aqueous solution are used successively to perform segmented percolation on the saffron raw material, which can fully extract the effective ingredients in saffron and further enhance the repair and whitening effects of tomato flower extract.

[0178] From the comparison of the data of Application Example 1 and Application Examples 9-11, it can be seen that in the preparation process of saffron extract, the flocculation treatment of saffron extract by using a combination of chitosan, sodium carboxymethyl starch and gelatin as a flocculant can further enhance the enrichment effect of the effective ingredients, improve the teeth repair and whitening effects of the extract, and at the same time improve the stability of the product.

[0179] The applicant declares that the present invention is illustrated by the above-described embodiments, but the present invention is not limited to the above-described embodiments. This does not mean that the present invention must rely on the above-described embodiments in order to be implemented. Those skilled in the art should understand that any improvements to the present invention, equivalent replacements for raw materials in the present invention, additions of auxiliary ingredients, and selection of specific methods, etc., fall within the scope of protection and disclosure of the present invention.

[0180] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the scope of protection of the present invention.

[0181] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any appropriate manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A composition having the effect of repairing and whitening teeth, characterized in that: The composition includes paeonol, honokiol, allantoin and crocus extract.

2. The composition according to claim 1, characterized in that Calculated by mass, the composition comprises 1-5 parts of paeonol, 1-5 parts of honokiol, 5-10 parts of allantoin and 0.5-1 part of saffron extract.

3. The composition according to claim 1 or 2, characterized in that The saffron extract is prepared by a preparation method comprising the following steps: (1) mixing saffron raw material with ethanol aqueous solution, performing percolation extraction, and collecting the extract to obtain a saffron crude extract; (2) The crude saffron extract is mixed with a flocculant for flocculation, and the supernatant is collected by centrifugation to obtain a saffron extract.

4. The composition according to claim 3, characterized in that The saffron raw materials in step (1) include saffron stigmas and saffron petals; Preferably, the mass ratio of the saffron stigma to the saffron petals is 1:(0.1-0.5).

5. The composition according to claim 3 or 4, characterized in that The percolation extraction method in step (1) is segmented percolation extraction; The method of the segmented percolation extraction comprises: (I) mixing the crocus raw material with an ethanol aqueous solution having a volume fraction of 80-90%, and performing percolation extraction to obtain an extract I; (II) mixing the crocus raw material with a 50-60% by volume ethanol aqueous solution, and performing percolation extraction to obtain extract II; Combine the extracts I and II to obtain a crude saffron extract; Preferably, the material-liquid ratio of the saffron raw material to the ethanol aqueous solution with a volume fraction of 80-90% in step (I) is 1:(10-30) g / mL; Preferably, the percolation extraction temperature in step (I) is 20-40° C., and the percolation extraction flow rate is 2-4 mL / min; Preferably, in step (II), the material-liquid ratio of the saffron raw material to the ethanol aqueous solution with a volume fraction of 50-60% is 1:(10-30) g / mL; Preferably, the temperature of the percolation extraction in step (II) is 20-40° C., and the flow rate of the percolation extraction is 2-4 mL / min.

6. The composition according to any one of claims 3 to 5, characterized in that The flocculant in step (2) comprises chitosan, sodium carboxymethyl starch and gelatin; Preferably, the mass ratio of chitosan, sodium carboxymethyl starch and gelatin is 1:(1-5):(0.1-1); Preferably, the mass ratio of the saffron crude extract to the flocculant is 100:(0.1-2).

7. A toothpaste with teeth repairing and whitening effects, characterized in that: The toothpaste comprises the composition with tooth repairing and whitening effects according to any one of claims 1 to 6, a surfactant, an abrasive, an anticaries agent, a thickener, a chelating agent, a moisturizer and a solvent.

8. The toothpaste according to claim 7, characterized in that The toothpaste comprises, by mass, 1-5 parts of the composition with tooth repairing and whitening effects according to any one of claims 1 to 6, 2-10 parts of a surfactant, 10-20 parts of an abrasive, 0.1-1 part of an anticaries agent, 1-5 parts of a thickener, 0.1-1 part of a chelating agent, 20-40 parts of a moisturizer, and 20-65 parts of a solvent.

9. The toothpaste according to claim 7 or 8, characterized in that The abrasive agent includes hydrated silica; Preferably, the surfactant comprises cocamidopropyl betaine; Preferably, the anticaries agent comprises sodium fluoride; Preferably, the thickener comprises cellulose gum and / or carbomer; Preferably, the chelating agent comprises tetrasodium pyrophosphate; Preferably, the moisturizing agent comprises any one or a combination of at least two of sorbitol, glycerol or polyethylene glycol; Preferably, the solvent comprises water.

10. A method for preparing a toothpaste having teeth repairing and whitening functions according to any one of claims 7 to 9, characterized in that: The preparation method comprises the following steps: (i) mixing paeonol, honokiol, and a surfactant to obtain a first mixture; and mixing allantoin, crocus extract, an abrasive, an anticaries agent, a thickener, a chelating agent, a moisturizer, and a solvent to obtain a second mixture; (ii) mixing the first mixture and the second mixture to obtain the toothpaste having teeth repairing and whitening effects; Preferably, the mixing and stirring temperature before obtaining the first mixture in step (i) is 20-40° C., and the mixing and stirring time is 5-10 min; Preferably, the mixing and stirring temperature before obtaining the second mixture in step (i) is 40-70° C., and the mixing and stirring time is 5-10 min; Preferably, the mixing and stirring temperature in step (ii) is 20-40° C., and the mixing and stirring time is 10-20 min.

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