Artificial climate box for diapause maintenance of procambarus clarkii and application of artificial climate box

Abstract

The invention belongs to the technical field of animal molecular marker screening and application. The artificial climate box for diapause maintenance of the procambarus clarkii comprises a cabinet body, a culture system, a water circulation system, a temperature control system, an oxygenation system and an electric power system. The cabinet body realizes efficient heat insulation. The breeding system is divided into a plurality of independent storage bins through transverse and vertical stainless steel filter screens, and brushes are arranged to increase the attachment surface of shrimp seeds. The water circulation system is combined with the water filtering device and the inner and outer double-pipe type dirt collecting device to achieve multi-stage filtering and removing. The power system is provided with a power-off alarm and a standby power supply to guarantee continuous operation of the system. The diapause state of the procambarus clarkii larvae can be effectively induced and prolonged by cooperatively regulating and controlling water cleanliness, low-temperature stability and dissolved oxygen sufficiency, intensive long-term storage of the procambarus clarkii larvae is achieved, seasonal supply limitation is broken through, and finally the industrial target that the procambarus clarkii larvae exist in four seasons and the procambarus clarkii exists in four seasons is achieved.

Description

Technical Field

[0001] The invention belongs to the technical field of aquaculture equipment, and in particular relates to an artificial climate box for maintaining diapause of Procambarus clarkii and application thereof. Background Art

[0002] As an important freshwater shrimp species, Procambarus clarkii is highly sought after for its delicious meat. With the continued expansion of Procambarus clarkii aquaculture, it has become a major aquaculture species. The optimal growth temperature for Procambarus clarkii is 20-30°C. Its growth and reproduction are severely hampered when water temperatures exceed 32°C, and it enters diapause at temperatures below 10°C.

[0003] The supply of Procambarus clarkii fry is concentrated from March to May, while the peak season for finished shrimp is in June. This seasonal imbalance between supply and demand severely restricts the healthy development of the industry. In particular, the shortage of fry in July and August leads to an insufficient supply of finished shrimp in winter, forming a vicious cycle of "lack of fry in summer and lack of shrimp in winter." If the fry of Procambarus clarkii can be maintained in diapause for a long time, the available period of the fry can be effectively extended and the development process can be precisely regulated. However, there is currently no mature technology or related equipment for maintaining diapause in fry of Procambarus clarkii. Therefore, the development of an artificial climate chamber for low-temperature induced diapause is of urgent industrial value to ensure the supply of shrimp fry throughout the year. Summary of the Invention

[0004] The purpose of the present invention is to overcome the defects of the prior art. In response to the above problems, the present invention provides an artificial climate chamber for maintaining diapause of Procambarus clarkii, which can achieve diapause induction and maintenance while ensuring the survival of shrimp seedlings by precisely controlling the diapause environmental conditions.

[0005] The technical solution of the present invention is as follows:

[0006] An artificial climate box for maintaining diapause of Procambarus clarkii comprises at least a cabinet, a culture system, a water circulation system, a temperature control system, an oxygenation system and an electric power system;

[0007] The climate chamber is surrounded by a cabinet body. The upper, lower, left, right and rear side panels of the cabinet body are all made of high-density foam insulation material. The front side uses a double-layer laminated tempered glass door for thermal insulation. Stainless steel cabinet feet are provided on the outside of the bottom of the cabinet body for support. Wire holes are provided on the upper part of the rear side panel of the cabinet body and water inlet and drainage holes are provided on the front of the lower side panel for power wiring and water inlet and drainage pipe layout.

[0008] The above-mentioned breeding system includes three layers of vertically stacked glass tanks, specifically, the first, second, and third tanks from bottom to top; the left, right, and rear sides of each tank are spliced ​​with glass panels of the same height, and the height of the front glass panel is two-thirds of the left / right glass panels; glass strips are provided on the inner sides of the front and rear glass panels inside the tank, and horizontal filters are laid on the top of the glass strips; a number of glass blocks are fixed above the horizontal filters, and vertical filters are inserted between the glass blocks to form multiple independent storage bins; a slot is provided on the top of the vertical filter, and a brush is installed in the slot; a water temperature sensor is also installed in the middle of each tank.

[0009] The above-mentioned water circulation system includes multiple water pipes and multi-layer water filtering devices arranged in the cylinder body; specifically, a water inlet pipe and a drain pipe are provided at the bottom opening on the left front side of the cabinet body, and the water inlet pipe is connected to the front side of the first cylinder body; a water pump is provided on the right side of the first cylinder body, and the output end is connected to the delivery pipe, which passes through the first cylinder body, extends vertically upward along the right side, and then extends horizontally through the top to the water filtering device on the left side of the third cylinder body; a double-tube leakage sewage collection device is provided on the right side of the third cylinder body, the inner water pipe passes through the bottom of the cylinder body and leads to the lower water filtering device, and the outer water pipe is fixed on the inner side of the bottom of the cylinder body, with an open lower end and an upper end 50 mm higher than the upper end of the inner water pipe; the third cylinder water leakage device is connected to the second cylinder water filtering device, and the second cylinder left side water leakage device is connected to the first cylinder water filtering device; the left front opening at the bottom of the third cylinder body is connected to the drain pipe.

[0010] The combination of aquaculture system and water circulation system achieves the following technical effects:

[0011] Three-dimensional space optimization: Horizontal + vertical filters form an adjustable breeding chamber, combined with brushes to slow down the impact of water flow and avoid squeezing of shrimp fry (density increased by 3 times); the overhead structure allows residual bait and feces to fall under the horizontal filter, keeping the storage chamber clean.

[0012] Efficient water circulation: When leftover bait accumulates, the water pump is started. The water flow path is: first cylinder → third cylinder → water leakage device → second cylinder → first cylinder, realizing multi-stage filtration and efficient cleaning. When the temperature difference between each cylinder is greater than 0.5℃, the water circulation is started to equalize the temperature. When changing water, the inlet / drain valve is opened synchronously to maintain flow balance and consistent inlet and outlet volume.

[0013] In the prior art, shrimp fry are cultured in a single space. Under the influence of water flow, the shrimp fry are easily gathered and squeezed at the bottom, and are prone to accidentally injuring each other.

[0014] Through comparative analysis, the technical solution of this application supplements the deficiencies of the existing technology through a series of closely related and mutually reinforcing structures, such as: the overhead three-dimensional adjustable warehouse achieves a significant increase in the breeding density per unit space and separates pollutants, the brush buffer reduces the stress of shrimp fry, and the specific water circulation paths between multiple cylinders and the unique drainage and filtration device significantly improve the water quality management efficiency.

[0015] The temperature control system includes an air temperature sensor and a water temperature sensor fixedly installed in the middle of the cabinet; the air temperature sensor is connected to a controller; the controller controls the compressor; the compressor is connected to a condenser; the condenser is arranged on the top of the cabinet; several fans are installed on both sides of the condenser, with the front fan supplying air upward and the rear fan supplying air downward.

[0016] The water temperature sensor and air temperature sensor work continuously, and dual sensor monitoring provides accurate temperature signals to the controller; the controller compares the set temperature value, and if it determines that cooling is required, it starts the compressor to cool the condenser; otherwise, the compressor stops working; the fan continues to run to accelerate air circulation.

[0017] The above design achieves the following innovative advantages: the compressor provides cooling for the condenser; the front fan draws the higher-temperature air inside the cabinet upward, passes through the condenser for cooling, and then blows cold air downward by the rear fan, slowly cooling the water through cold air convection; this cooling method can reduce the crayfish's stress response to changes in water temperature; the internal circulation air cooling structure facilitates precise temperature control and reduces energy consumption.

[0018] Conventional systems often rely on a single water temperature sensor, unable to sense air temperature gradients. This results in temperature control lag (deviations exceeding ±1°C), making it difficult to meet the sensitive needs of crustaceans. Direct water cooling or high-power air cooling can easily cause sudden drops in water temperature (>0.5°C / minute), triggering stress reactions in shrimp. This solution achieves precise temperature control and ultra-slow temperature changes through a closed-loop collaborative mechanism of "dual-dimensional monitoring → intelligent decision-making → asymmetric airflow → indirect slow cooling."

[0019] The above-mentioned oxygenation system at least includes an aerator, an oxygenation diverter, and a sub-oxygen supply pipe; the aerator is connected to the main oxygen supply pipe and extends into the cabinet; the other end of the main oxygen supply pipe is connected to the oxygenation diverter; the oxygenation diverter is connected to several sub-oxygen supply pipes; the end of each sub-oxygen supply pipe is connected to a biochemical cotton filter; each cylinder is equipped with a set of sub-oxygen supply pipes and biochemical cotton filters to achieve independent oxygen supply and local water filtration for each breeding cylinder.

[0020] The power system includes a power outage alarm and a backup power supply. The external power supply charges the backup power supply via the power outage alarm. The backup power supply powers the lighting, water circulation, temperature control, and oxygenation systems, all connected in parallel. In the event of a power outage, the power outage alarm issues a signal via phone, text message, and audible and visual alarms. The backup power supply can maintain the climate chamber's operation for 10 hours or more, ensuring a stable aquaculture environment. The lighting system is installed on the left, right, and top front of the cabinet to facilitate monitoring of aquaculture status.

[0021] The centralized aeration in traditional aquaculture systems leads to a distance attenuation effect, and the dissolved oxygen content in the terminal aquaculture unit drops sharply; traditional oxygenation devices such as air stones have no filtering function, and leftover bait debris rises with the bubbles to secondary pollute the water body; traditional aquaculture equipment has low redundancy and short response time in the event of power outages and loss of control.

[0022] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0023] (1) Independent oxygen supply closed loop: Each cylinder is equipped with a dedicated oxygen supply pipe to ensure that the difference in dissolved oxygen content in each cylinder is reduced and the problem of uneven oxygen supply is solved.

[0024] (2) Fusion of oxygenation and filtration functions: When oxygen passes through the filter cotton, it simultaneously adsorbs organic debris, and the measured suspended solids removal rate is increased by 40% (compared with the traditional pure oxygen system), thereby inhibiting eutrophication of water bodies.

[0025] (3) Build a full-link protection system of "early warning-power supply-monitoring": For the first time, zero-disturbance control of the biological environment in power outage scenarios has been achieved in aquaculture equipment. This design has been verified: in a 72-hour power outage simulation experiment, the survival rate of Procambarus clarkii seedlings increased from 40% in the traditional system to 98%, and there were no stress-induced molting abnormalities, which has the value of disruptive innovation in the industry. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 The present invention provides a side view of the structure of an artificial climate box for maintaining diapause of Procambarus clarkii;

[0027] Figure 2 The present invention provides a side structural perspective diagram of an artificial climate chamber for maintaining diapause of Procambarus clarkii with part of the cabinet glass door and baffle removed;

[0028] Figure 3 The present invention provides a three-dimensional exploded view of the cabinet part of an artificial climate box for maintaining diapause of Procambarus clarkii;

[0029] Figure 4 A partial three-dimensional exploded diagram of a culture space system and a water circulation system in an artificial climate box for maintaining diapause of Procambarus clarkii proposed by the present invention;

[0030] Figure 5 The present invention proposes a three-dimensional disassembled diagram of the culture space system and water circulation system in an artificial climate box for maintaining diapause of Procambarus clarkii without the front glass plate of the tank;

[0031] Figure 6 A partial exploded perspective view of a water circulation system in an artificial climate chamber for maintaining diapause of Procambarus clarkii proposed in the present invention;

[0032] Figure 7A partial three-dimensional exploded diagram of a temperature control system in an artificial climate box for maintaining diapause of Procambarus clarkii proposed in the present invention;

[0033] Figure 8 The present invention provides a three-dimensional exploded diagram of an oxygenation system and a part of a culture space system in an artificial climate box for maintaining diapause of Procambarus clarkii;

[0034] Figure 9 The present invention proposes a three-dimensional exploded diagram of the power system in an artificial climate box for maintaining diapause of Procambarus clarkii;

[0035] Among them: 1-cabinet; 2-breeding system; 3-water circulation system; 4-temperature control system; 5-oxygenation system; 6-power system; 1-1, double-layer laminated tempered glass door; 1-2, cabinet upper side panel; 1-3, cabinet rear side panel; 1-4, cabinet right side panel; 1-5, cabinet lower side panel; 1-6, cabinet foot; 2-1, first cylinder; 2-2, second cylinder; 2-3, third cylinder; 2-3-1, cylinder 2-3-2, left side glass panel; 2-3-3, right side glass panel; 2-3-4, bottom side glass panel; 2-3-5, front side glass panel; 2-3-6, glass strip; 2-3-7, horizontal filter; 2-3-8, glass block; 2-3-9, vertical filter; 2-3-10, water temperature gauge; 3-1-1, water pump; 3-1-2, water pump power cord; 3-2 , water delivery pipe; 3-3, water filtration device; 3-4, water leakage device; 3-4-1, water pipe outside the leaking device; 3-4-2, water pipe inside the leaking device; 3-5, water inlet pipe; 3-6, drain pipe; 4-1, temperature control system power cord; 4-2, compressor; 4-3, condenser; 4-4, fan; 4-5, controller; 4-6, air temperature sensor; 5-1, aerator power cord; 5-2, aerator ; 5-3, main oxygen supply pipe; 5-4, oxygenation diverter; 5-5-1, first cylinder sub-oxygen supply pipe; 5-5-2, second cylinder sub-oxygen supply pipe; 5-5-3, third cylinder sub-oxygen supply pipe; 5-6, biochemical cotton filter; 6-1, power off alarm; 6-2, backup power supply; 6-3, power strip; 6-4-1, lighting system power cord; 6-4-2, top lighting system; 6-4-3, side lighting system. DETAILED DESCRIPTION

[0036] The embodiments of the present application are described in detail below with reference to the accompanying drawings.

[0037] In the description of this application, it should be understood that the terms "center", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on this application.

[0038] The terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of the technical features being referred to. Thus, a feature specified as "first" or "second" may explicitly or implicitly include one or more of such features. Throughout this application, unless otherwise specified, "plurality" means two or more.

[0039] In the description of this application, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be internal communication between two components. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances. The following is a further installation description of the present invention in conjunction with the drawings and embodiments. It should be noted that the embodiments of this application and the features in the embodiments can be combined with each other unless there is a conflict.

[0040] like Figure 1-3 As shown, the artificial climate chamber for diapause maintenance of Procambarus clarkii consists of a cabinet 1, a culture system 2, a water circulation system 3, a temperature control system 4, an oxygenation system 5, and an electrical system 6. Cabinet 1 is constructed of high-density foam insulation and stainless steel plates. The panels on the top, bottom, left, right, and rear sides are all ≥50 mm thick. The internal dimensions of cabinet 1 are determined by the size of the culture system 2 to be housed. The front door features a double-layered laminated tempered glass door 1-1, edged with a high-density rubber seal to effectively isolate the environment from heat. Adjustable stainless steel feet 1-6 are installed at the bottom of cabinet 1, providing space for inlet and outlet pipes and a gap to prevent water accumulation.

[0041] like Figure 4-5As shown, the aquaculture system 2 is housed within a cabinet 1 and comprises three vertically stacked glass tanks (from bottom to top: first tank 2-1, second tank 2-2, and third tank 2-3). Each tank achieves three-dimensional aquaculture through the following structure: Left, right, and rear glass panels are spliced ​​together at equal heights to form a basic framework, with the front glass panel 2-3-5 reduced to two-thirds the height of the side panels to facilitate feeding and observation. Glass strips 2-3-6, with a height greater than 30 mm from the bottom, are installed inside the front and rear glass panels. A 40-mesh horizontal filter 2-3-7 is placed above the strips to create a channel for dropping waste and leftover bait. Arrayed glass blocks 2-3-8 are bonded above the horizontal filter 2-3-7, with 40-mesh vertical filters 2-3-9 inserted between the blocks, dividing the tanks into independent, adjustable aquaculture chambers and increasing the three-dimensional living space for the crayfish. A slot is provided on the top of the vertical filter 2-3-9 to accommodate a brush, which mitigates water flow impact by increasing the surface area for biological attachment and prevents crushing and damage to the shrimp fry.

[0042] In this embodiment, the leftover bait and feces produced during the breeding process fall under the horizontal filter screen 2-3-8. When a certain amount of leftover bait and feces accumulates under the horizontal filter screen 2-3-8, the water circulation system 3 is started, and the water pump 3-1-1 in the water circulation system 3 pumps the water from the first cylinder 2-1 to the third cylinder 2-3 through the water delivery pipe 3-2. The water passes through the water filter device 3-3 of the third cylinder for the first filtration, and the leftover bait and feces at the bottom of the third cylinder flows with the water flow to the water leakage device 3-4 of the third cylinder. The leftover bait and feces pass through the opening below the outer water pipe 3-4-1 of the drainage device, and are discharged into the water filter device 3-3 of the second cylinder 2-2 through the inner water pipe 3-4-2 of the suction device for filtration. This process is analogous to the formation of a closed-loop multi-stage filtration to ensure a clean water quality environment.

[0043] In this embodiment, a water thermometer 2-3-10 is installed in the middle of each cylinder to monitor the water temperature in real time throughout the day. When the water temperature difference between the cylinders is greater than 0.5°C, the water circulation 3 is automatically started to circulate and mix the water in each cylinder, thereby achieving a uniform temperature effect, reducing water temperature differences, and ensuring the uniformity of diapause temperature.

[0044] like Figure 5 As shown, during the water change operation, the water inlet pipe 3-5 and the drain pipe 3-6 (located at the lower side 1-5 of the cabinet and the bottom of the first cylinder) are opened simultaneously, and equal water change is achieved through flow balance control to avoid water level fluctuations causing stress to the shrimp fry.

[0045] like Figure 8 As shown, the oxygenation system 5 improves water quality through physical filtration: the oxygen output by the aerator 5-2 is transported to the oxygenation diverter 5-4 through the main oxygen supply pipe 5-3; the diverter distributes the oxygen to the sub-oxygen supply pipes (5-5-1 / 5-5-2 / 5-5-3), and the ends are connected to the biochemical cotton filter 5-6; oxygen is released from the center of the filter to form a negative pressure area, driving the surrounding water flow to penetrate the biochemical cotton layer, and adsorbing residual pollutants while increasing oxygen, thereby achieving local purification of the breeding tank.

[0046] like Figure 7 As shown, the temperature control system 4 is a split type. The compressor 4-2 is external to reduce thermal interference. The fan 4-4, controller 4-5, and air temperature sensor 4-6 are installed on the front side of the cabinet top. The compressor 4-2 is connected to both ends of the condenser 4-3. The condenser 4-3 passes into the interior of the cabinet 1 through the opening 1-3 on the rear side of the cabinet. The condenser 4-3 is coiled around the cabinet top in a serpentine shape to increase the heat exchange area. Several fans 4-4 are installed on both sides of the condenser 4-3. The front and rear fans 4-4 form a directional airflow (suction in front and blowing out from behind).

[0047] Water thermometer 2-3-10 and air temperature sensor 4-6 form a dual monitoring system that accurately monitors aquaculture temperatures and transmits signals to controller 4-5. Controller 4-5 compares real-time data with the set value (7-10°C diapause range). If cooling is determined, controller 4-5 activates compressor 4-2, which then cools condenser 4-3. The front fan absorbs the heated air, which is then cooled by the condenser. The rear fan then blows cool air downward, initially lowering the air temperature inside the cabinet. Continuous fan operation ensures air circulation, with a water temperature change rate of ≤0.5°C / h, significantly reducing thermal stress on the shrimp fry. Internal circulation air cooling also ensures precise temperature control. Conversely, compressor 4-2 stops, while fan 4-4 remains operational to maintain air circulation.

[0048] like Figure 9 As shown, the power supply of the power system 6 is first connected to the power outage alarm 6-1 to supply power to the backup power supply 6-2, which in turn provides power to the lighting systems 6-4-2 and 6-4-3, the water circulation system 3, the temperature control system 4, and the oxygenation system 5. In the event of a power outage, the power outage alarm 6-1 can send a power outage signal via phone, text message, or alarm, ensuring timely detection of the outage. In the event of a power outage, the backup power supply 6-2 can independently maintain the artificial climate chamber for more than 10 hours, ensuring that the breeding environment in the climate chamber is not affected. The lighting system is installed on the top 6-4-2 and side 6-4-3 of the cabinet to facilitate monitoring of the breeding conditions.

[0049] It should be noted that, unless there is a conflict, the embodiments and features in the embodiments of this application can be combined with each other. The above embodiments are only used to illustrate the technical solutions of this application and are not intended to limit this application. For those skilled in the art, this application may have various changes and variations. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of this application should be included in the scope of protection of this application.

Claims

1. An artificial climate chamber for maintaining diapause of Procambarus clarkii, characterized in that: The artificial climate chamber includes a cabinet, a breeding system, a water circulation system, a temperature control system, an oxygenation system and a power system; The artificial climate box is surrounded by a cabinet body, which is composed of an upper panel, a lower panel, a left panel, a right panel and a rear panel. The front side of the cabinet body is provided with a double-layer laminated tempered glass door, and the bottom of the cabinet body is provided with a stainless steel foot support. The breeding system comprises: a vertically stacked multi-layer glass cylinder, preferably divided into a first cylinder, a second cylinder, and a third cylinder from bottom to top; The water circulation system includes a water pump, a water pipe, a water filter, and a water leakage and sewage collection device; The temperature control system includes an air temperature sensor and a water temperature sensor in the cabinet.

2. The artificial climate chamber according to claim 1, characterized in that The panel material of the cabinet body is a high-density foam insulation material. The upper part of the rear panel is provided with a threading hole, and the front part of the lower panel is provided with a drainage pipe hole.

3. The artificial climate chamber according to claim 1, characterized in that The left side, right side and rear side of each cylinder body are spliced ​​by glass panels of the same height, and the height of the front glass panel is two-thirds of the left / right glass panels; glass strips are provided on the inner sides of the front and rear glass panels inside the cylinder body, and horizontal filter screens are laid on the upper part of the glass strips; a slot is provided on the upper part of the vertical filter screen, and a brush is installed in the slot; preferably, a number of glass blocks are fixed above the horizontal filter screen, and vertical filter screens are inserted between the glass blocks to form multiple independent storage bins.

4. The artificial climate box according to claim 1, 2 or 3, characterized in that: The water circulation system is specifically as follows: a water inlet pipe and a drain pipe are connected at the bottom of the left front side of the cabinet, and the water inlet pipe is connected to the front side of the first cylinder; a water pump is provided on the right side of the first cylinder, and its output end is connected to the water delivery pipe; the water delivery pipe passes through the glass plate on the right side of the first cylinder in sequence, extends vertically upward along the right side of the cylinder, and then extends horizontally along the top to the water filtering device on the left side of the third cylinder; a leakage sewage collecting device is provided on the right side of the third cylinder, including an inner water pipe passing through the bottom of the cylinder and an outer water pipe fixed to the inner side of the bottom of the cylinder; the lower end of the outer water pipe is open, and the upper end is higher than the upper end of the inner water pipe; the leakage sewage collecting device of the third cylinder is connected to the water filtering device on the right side of the second cylinder, and the leakage sewage collecting device on the left side of the second cylinder is connected to the water filtering device on the left side of the first cylinder; the left front side of the bottom of the third cylinder is connected to the drain pipe.

5. The artificial climate chamber according to claim 1, 2 or 3, characterized in that: The temperature control system is specifically as follows: an air temperature sensor fixed in the middle of the cabinet is connected to a controller, a compressor controlled by the controller, a condenser connected to the compressor, and the condenser extends to the top of the rear side of the cabinet; a number of fans are installed on both sides of the condenser, the front fan supplies air upward, and the rear fan supplies air downward.

6. The artificial climate chamber according to claim 5, characterized in that: The oxygen enrichment system includes: an oxygenator connected to a main oxygen supply pipe entering a cabinet, the main oxygen supply pipe is connected to an oxygen diverter; the oxygen diverter divides into multiple sub-oxygen supply pipes, and the end of each sub-oxygen supply pipe is connected to a biochemical cotton filter; the biochemical cotton filters are distributed in the storage bins of each cylinder.

7. The artificial climate chamber according to claim 1, characterized in that: In the power system, the external power supply supplies power to the backup power supply through the power off alarm; the backup power supply supplies power to the lighting system, water circulation system, temperature control system and oxygenation system; the lighting system, water circulation system, temperature control system and oxygenation system are connected in parallel.

8. Use of the artificial climate chamber according to claim 1 in maintaining diapause of Procambarus clarkii.

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