Modular cell culture container and use method thereof

Abstract

The invention discloses a modular cell culture container and a use method thereof, and belongs to the technical field of cell culture.The modular cell culture container is provided with a rotating shaft, a rotating motor, a clamping ring, a positioning clamping groove, a fixing clamping base, a fixing spring and a culture dish, the fixing clamping base covers the top of the culture dish and the top of a positioning clamping plate, and the culture dish is clamped and fixed into the clamping ring; after a plurality of culture dishes are respectively clamped and fixed in corresponding clamping rings, and the culture dishes are uniformly distributed on the surface of a rotating shaft, a magnetic door plate is closed, a rotating motor is started, and the rotating shaft is controlled to rotate in the incubator through meshing of a bevel gear II and a bevel gear I; a plurality of culture dishes are driven to rotate at a constant speed in the culture box and uniformly receive light rays emitted by the illuminator, so that obvious errors of cells cultured in the culture dishes caused by obvious difference of intensity and duration of the light rays received by the culture dishes at different positions in the culture box are avoided.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture, and in particular to a modular cell culture container and a use method thereof. Background Art

[0002] Cell culture vessels are important tools in the field of cell research and application. From basic cell biology research, exploring the basic life activities of cells such as growth, differentiation, and metabolism, to the construction of cell models used in drug development to screen potential effective drugs and evaluate drug toxicity, to the key links of tissue engineering and cell therapy in the field of regenerative medicine, cell culture is an indispensable core technology. Placing cells in cell culture vessels provides a suitable growth environment for cell culture, promotes cell growth and reproduction, and facilitates scientific researchers to observe and operate cells. At the same time, cell culture vessels can also achieve large-scale cell culture and simulate the in vivo microenvironment, providing important technical support for biomedical research and applications.

[0003] When conducting cell culture in existing cell culture containers, the culture dish is directly placed inside the incubator. When the incubator is hit and shaken, the culture dish is likely to shake simultaneously inside the incubator, causing the cells inside the culture dish to leak. At the same time, the culture dish is placed in different positions inside the incubator, and the culture dish is exposed to light for different times and intensities, which can easily lead to large deviations in the cell culture results. At the same time, since different cells require light conditions of different wavelengths and types when being cultured, the illuminator is generally directly fixed inside the incubator and can only emit light of a single type and intensity, which is difficult to meet the lighting needs of different cell cultures. At the same time, the illuminator is installed inside the incubator and is not easy to disassemble. Therefore, improvement is needed.

[0004] Based on this, the present invention designs a modular cell culture container and a method of using the same to solve the above problems. Summary of the Invention

[0005] The object of the present invention is to provide a modular cell culture container and a method for using the same, so as to solve the problem proposed in the above background technology that in the existing cell culture container, when cell culture is carried out, the culture dish is directly placed inside the incubator. When the incubator is hit and shaken, the culture dish is likely to shake synchronously inside the incubator, resulting in leakage of cells inside the culture dish. At the same time, the culture dish is placed at different positions inside the incubator, and the culture dish is exposed to light for different times and intensities, which can easily lead to large deviations in the cell culture results. At the same time, since different cells need to be cultured under light conditions of different wavelengths and types, since the illuminator is generally directly fixed inside the incubator, it can only emit light of a single type of intensity, which is difficult to meet the illumination needs of different cell cultures. At the same time, the illuminator is installed inside the incubator and is not easy to disassemble.

[0006] To achieve the above object, the present invention provides the following technical solutions: A modular cell culture container includes an incubator, wherein a rotating shaft is rotatably installed between the top and bottom of the inner side of the incubator via a bearing, the top end of the rotating shaft passes through the incubator and is fixedly installed with a bevel gear 1, a rotating motor is fixedly installed on one side of the top of the incubator, and a bevel gear 2 is fixedly installed on the output end of the rotating motor, and the bevel gear 2 is meshed with the bevel gear 1, a plurality of mounting blocks are fixedly installed on the surface of the rotating shaft located on the inner side of the incubator, a culture dish is clamped on the end of the mounting block away from the rotating shaft, a mounting mechanism is fixedly installed at a central position on one side of the incubator, and an illuminator is clamped inside the mounting mechanism.

[0007] As a further solution of the present invention, a rectangular groove is provided on one side of the incubator, a magnetic door panel is rotatably mounted on one side of the opening of the incubator via a pin, and a sealing strip is fixedly mounted on the surface of the magnetic door panel.

[0008] As a further solution of the present invention, a clamping ring is symmetrically fixedly installed on one end of the mounting block away from the rotating shaft, a positioning slot is provided at the top center position of the mounting block close to the clamping ring, a fixed clamping seat is slidably installed through the surface of the mounting block, mounting slots 1 are symmetrically provided on both sides of the mounting block, and sliding slots are provided on both sides of the mounting block close to the clamping ring, and the sliding slots are connected to mounting slot 1.

[0009] As a further solution of the present invention, a mounting rod is fixedly installed on the inner wall of the mounting groove, a fixed pressure ring is slidably installed on the surface of the mounting rod, a fixed spring is sleeved on the surface of the mounting rod on one side of the fixed pressure ring, a connecting protrusion is fixedly installed on the surface of the fixed pressure ring, and the connecting protrusion is slidably connected to the inside of the slide groove, and the end of the connecting protrusion away from the fixed pressure ring is fixedly connected to the inner wall of the fixed bracket.

[0010] As a further solution of the present invention, a positioning card is fixedly installed on the top of the culture dish surface, and the positioning card is clamped in the positioning card slot. A buckle slot is provided on one side of the positioning card.

[0011] As a further solution of the present invention, the mounting mechanism includes two mounting bars, and the mounting bars are fixedly mounted on both sides of the inner wall of the rectangular groove. Mounting slots are respectively provided at both ends of the mounting bar, and a moving block is slidingly installed inside the mounting slot. A rubber pad is fixedly installed on one side of the moving block, and the rubber pad is tightly attached to the inside of the mounting slot. The moving blocks inside the mounting slot at the top or bottom of the two mounting bars extend through the mounting slot to the outside of the mounting bar and are fixedly installed with a connecting strip.

[0012] As a further solution of the present invention, a second mounting groove is provided at the central position of the mounting bar, and two mounting rods are symmetrically fixedly installed on both sides of the second mounting groove. A movable top plate is slidably installed on the surface of the second mounting rod, and a lifting spring is sleeved on the surface of the second mounting rod on the side of the movable top plate. A top block is fixedly installed between the two second mounting rods, and the top block passes through the second mounting groove and extends to the outside of the mounting bar.

[0013] As a further solution of the present invention, L-shaped card plates are fixedly installed on the top and bottom of one side of the surface of the illuminator, and the connecting card strips are clamped on the inner side of the L-shaped card plates. Side panels are symmetrically fixedly installed on both sides of the illuminator, and the side panels are overlapped on the surface of the top block corresponding to the position of the second installation groove.

[0014] A method for using a modular cell culture container, comprising the following steps: Select the corresponding illuminator according to the light source required for different cell cultures, insert the illuminator between the two mounting bars, make the side panels on both sides of the illuminator snap into the corresponding mounting groove II and fit tightly against the top block, squeeze the top block into the mounting groove II, drive the movable top plates on both sides of the top block to slide on the surface of the corresponding mounting rod II, squeeze the top spring to deform, until the illuminator is completely inserted between the two mounting bars, horizontally insert the movable blocks at both ends of the connecting card bar into the corresponding mounting slot, make the rubber card pad fit tightly against the inner wall of the mounting slot, slide the connecting card bar down and snap into the L-shaped card plate, thereby firmly fixing the illuminator between the two mounting bars; The fixing clamp is pushed to slide on the surface of the mounting block until the fixing clamp is away from the clamping ring. At the same time, the fixing clamp controls the fixing pressure ring to slide on the surface of the mounting rod under the drive of the connecting protrusion, squeezing the fixing spring to deform, and the culture dish filled with cell culture fluid is inserted into the inside of the clamping ring. After the positioning clamp is inserted into the positioning slot, the fixing clamp is released. The fixing pressure ring is lifted up by the rebound action of the fixing spring and slides back on the surface of the mounting rod until the fixing clamp is moved and clamped on the top of the culture dish. The culture dish is clamped and fixed inside the clamping ring. Repeat the steps to clamp multiple culture dishes into the corresponding clamping rings. The magnetic door panel is rotated to fit tightly against the incubator, squeezing the sealing strip to deform it, thereby placing the interior of the incubator in a relatively closed environment. The illuminator is controlled to emit light suitable for culturing cells inside the culture dish, and the rotating motor is controlled to drive the second bevel gear to rotate. Under the meshing action of the second bevel gear and the first bevel gear, the rotating shaft rotates inside the incubator, driving multiple mounting blocks and culture dishes to rotate synchronously at a uniform speed inside the incubator, ensuring that the culture dishes pass through the illuminator at a uniform speed, so that the culture dishes at different positions on the surface of the rotating shaft are evenly illuminated by the light emitted by the illuminator, thereby preventing culture dishes at different positions inside the incubator from being exposed to light of different intensities and durations, resulting in large deviations in culture dish cultivation. When disassembling and replacing the illuminator, lift the connecting card strip away from the L-shaped card plate. After the two connecting card strips are away from the L-shaped card plate, the thrust on the illuminator is cancelled, and the second mounting rod is lifted under the rebound action of the lifting spring, so that the lifting block pushes the side plate out of the second mounting groove, thereby pushing the illuminator out of the two mounting bar brackets, making it convenient to disassemble and replace the illuminator, and replace the illuminator with different light according to different cell culture lighting conditions.

[0015] Compared with the prior art, the present invention has the following beneficial effects: 1. The present invention is provided with a rotating shaft, a rotating motor, a clamping ring, a positioning slot, a fixed clamping seat, a fixed spring and a culture dish. The fixed clamping seat is pushed to drive the fixed pressure ring to slide on the surface of the mounting rod to squeeze the fixed spring to deform until the fixed clamping seat is away from the clamping ring to expose the positioning slot. The culture dish is vertically placed into the clamping ring so that the positioning card plate is inserted into the corresponding positioning slot. The fixed clamping seat is released. Under the rebound action of the fixed spring, the fixed pressure ring is lifted up to slide on the surface of the mounting rod, so that the connecting protrusion slides inside the slide groove, driving the fixed clamping seat to slide on the surface of the mounting block until the fixed clamping seat covers the culture dish and the positioning card plate. At the top of the plate, the culture dish is clamped and fixed inside the clamping ring, so as to facilitate the removal and replacement of the culture dish. After the multiple culture dishes are clamped and fixed inside the corresponding clamping rings so that the culture dishes are evenly distributed on the surface of the rotating shaft, the magnetic door panel is closed, and the rotating motor is started. After the bevel gear 2 and the bevel gear 1 are meshed, the rotating shaft is controlled to rotate inside the incubator, driving the multiple culture dishes to rotate at a uniform speed inside the incubator, evenly receiving the light emitted by the illuminator, avoiding obvious differences in the intensity and duration of the light received by the illuminator for culture dishes at different positions inside the incubator, resulting in obvious errors in the cells cultured inside the culture dishes; 2. The present invention sets a moving block, a connecting clip, a lifting spring, a lifting block, an L-shaped clip and a side plate. After removing the two connecting clips from the surface of the mounting strips, the mobile illuminator is vertically suspended at the position of the two mounting strips so that the position of the illuminator corresponds to the position between the two mounting strips. The mobile illuminator is inserted between the two mounting strips so that the side plates on both sides of the illuminator are inserted into the second mounting groove, pushing the lifting block to retract into the second mounting groove, driving the mobile top plate to slide on the second surface of the mounting rod to squeeze the lifting spring to deform, and the mobile connecting clip is horizontally placed on the outside of the two mounting strips so that the moving block and the mounting strip are aligned. The position of the card slot corresponds to that of the card slot, and the moving block is inserted into the installation card slot until the rubber card pad is tightly attached to the inner wall of the installation card slot, and the connecting card strip is lowered to make the connecting card strip inserted into the inside of the L-shaped card plate, so that the illuminator is clamped and fixed between the two installation strips, which is convenient for installing the illuminator. When disassembling the illuminator, the connecting card strip is pulled out from the inside of the L-shaped card plate, and the movable top plate is lifted under the rebound action of the lifting spring, which drives the top block to push the side plate out of the second installation slot, so that the illuminator is pulled out between the two installation strips, which is convenient for disassembling the illuminator and replacing it with an illuminator that meets the light requirements of the culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0017] Figure 1 It is a schematic diagram of the expanded structure of the present invention; Figure 2 Schematic diagram of the expanded structure of the incubator of the present invention; Figure 3 Schematic diagram of the cross-sectional structure of the rotating shaft, the mounting block and the culture dish of the present invention; Figure 4 For the present invention Figure 3 Schematic diagram of the enlarged structure at A in the middle; Figure 5 This is a schematic structural diagram of the mounting block and the fixed base of the present invention; Figure 6 Schematic diagram of the structure of the culture dish and positioning card plate of the present invention; Figure 7 Schematic diagram of the cross-sectional structure of the mounting strip and the illuminator of the present invention; Figure 8 For the present invention Figure 7 Schematic diagram of the enlarged structure at B in the middle; Figure 9 For the present invention Figure 7 Schematic diagram of the enlarged structure at point C in the middle.

[0018] In the accompanying drawings, the components represented by the reference numerals are as follows: 1. Incubator; 101. Rectangular slot; 102. Magnetic door panel; 103. Sealing strip; 2. Rotating shaft; 3. Bevel gear 1; 4. Rotating motor; 5. Bevel gear 2; 6. Mounting block; 601. Snap ring; 602. Positioning slot; 603. Fixed base; 604. Mounting slot 1; 605. Slide; 606. Mounting rod 1; 607. Fixed pressure ring; 608. Fixed spring; 609. Connector Bump; 7. Culture dish; 701. Positioning card plate; 702. Buckle slot; 8. Mounting mechanism; 801. Mounting strip; 802. Mounting card slot; 803. Moving block; 804. Rubber pad; 805. Connecting card strip; 806. Mounting slot 2; 807. Mounting rod 2; 808. Moving top plate; 809. Lifting spring; 810. Top block; 9. Illuminator; 901. L-shaped card plate; 902. Side panel. DETAILED DESCRIPTION

[0019] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts shall fall within the scope of protection of the present invention.

[0020] See also Figures 1-9 , the present invention provides a technical solution: A modular cell culture container includes an incubator 1. A rotating shaft 2 is rotatably mounted between the top and bottom of the inner side of the incubator 1 via bearings. The top end of the rotating shaft 2 passes through the incubator 1 and is fixedly mounted with a bevel gear 1 3. A rotating motor 4 is fixedly mounted on one side of the top of the incubator 1. A bevel gear 2 5 is fixedly mounted on the output end of the rotating motor 4, and the bevel gear 2 5 meshes with the bevel gear 1 3. A plurality of mounting blocks 6 are fixedly mounted on the surface of the rotating shaft 2 located on the inner side of the incubator 1. A culture dish 7 is clamped to the end of the mounting block 6 away from the rotating shaft 2. A mounting mechanism 8 is fixedly mounted at a central position on one side of the incubator 1, and an illuminator 9 is clamped inside the mounting mechanism 8. During operation, a suitable illuminator 9 is selected according to the light requirements for cell culture, and the illuminator 9 is correspondingly clamped inside the mounting mechanism 8 so that the illuminator 9 emits light toward the inside of the incubator 1. Then, multiple culture dishes 7 are clamped at one end of the corresponding mounting block 6. After the incubator 1 is closed, the rotating motor 4 is started to control the bevel gear 2 5 to rotate. After the bevel gear 2 5 and the bevel gear 1 3 are meshed, the rotating shaft 2 is controlled to drive the mounting block 6 and the culture dishes 7 to rotate inside the incubator 1. At the same time, the illuminator 9 is started to emit light into the incubator 1 so that the culture dishes 7 pass through the illuminator 9 at a uniform speed, ensuring that the light emitted by the illuminator 9 is evenly irradiated on the surface of the culture dishes 7, so that the culture dishes 7 at different positions inside the incubator 1 are exposed to light of the same duration and intensity, thereby reducing the culture errors caused by the different illumination durations and intensities of the illuminator 9. As a further solution of the present invention, a magnetic door panel 102 is rotatably mounted on one side of the opening of the incubator 1 via a pin, and a sealing strip 103 is fixedly mounted on the surface of the magnetic door panel 102; During operation, after multiple culture dishes 7 are snap-connected to the mounting block 6, the magnetic door panel 102 is rotated to attract the magnetic door panel 102 and the incubator 1, squeezing the sealing strip 103 to deform, so that the interior of the incubator 1 is in a relatively closed environment, reducing the impact of the external environment on the cell culture in the culture dish 7 when the culture dish 7 is conducting cell culture.

[0021] As a further solution of the present invention, a clamping ring 601 is symmetrically fixedly installed on one end of the mounting block 6 away from the rotating shaft 2, a positioning slot 602 is provided at the top center position of the mounting block 6 near the clamping ring 601, and a fixed clamping seat 603 is slidably installed on the surface of the mounting block 6; a positioning clamping plate 701 is fixedly installed on the top of the surface of the culture dish 7, and the positioning clamping plate 701 is clamped in the positioning clamping slot 602, and a buckle groove 702 is provided on one side of the positioning clamping plate 701; When working, push the fixed clamping seat 603 away from the clamping ring 601 and clamp the culture dish 7 into the inside of the clamping ring 601. The top of the culture dish 7 has a flange placed on the flange inside the clamping ring 601, and the clamping ring 601 is semicircular, which is convenient for placing or removing the culture dish 7; make the positioning clamping plate 701 clamped in the positioning clamping groove 602, ensuring that the culture dish 7 is placed in the inside of the clamping ring 601 without position deviation. At the same time, the fixed clamping seat 603 covers the edge of the culture dish 7 and the top of the positioning clamping plate 701, so that the culture dish 7 is clamped and fixed in the inside of the clamping ring 601, which is convenient for placing and fixing the culture dish 7; when taking the culture dish 7 out of the inside of the clamping ring 601, push the fixed clamping seat 603 away from the culture dish 7 and the positioning clamping plate 701, and get your fingers stuck in the buckle groove 702, so that the buckle groove 702 is taken out from the inside of the positioning clamping groove 602, which is convenient for taking the culture dish 7 out of the inside of the clamping ring 601.

[0022] As a further solution of the present invention, mounting grooves 604 are symmetrically provided on both sides of the mounting block 6, and sliding grooves 605 are provided on both sides of the mounting block 6 near the clamping ring 601, and the sliding grooves 605 are connected to the mounting groove 604; a mounting rod 606 is fixedly installed on the inner wall of the mounting groove 604, and a fixed pressure ring 607 is slidably installed on the surface of the mounting rod 606, and a fixing spring 608 is sleeved on the surface of the mounting rod 606 on one side of the fixed pressure ring 607, and a connecting protrusion 609 is fixedly installed on the surface of the fixed pressure ring 607, and the connecting protrusion 609 is slidably connected to the inside of the sliding groove 605, and the end of the connecting protrusion 609 away from the fixed pressure ring 607 is fixedly connected to the inner wall of the fixed clamping seat 603; During operation, push the fixed card seat 603 to slide on the surface of the mounting block 6, so that the connecting protrusion 609 slides inside the slide groove 605, and drives the fixed pressure ring 607 to slide on the surface of the mounting rod 606, squeezing the fixed spring 608 to deform, until the fixed card seat 603 is away from the clamping ring 601 to expose the positioning card groove 602, which makes it convenient to put the culture dish 7 into the clamping ring 601, so that the positioning card plate 701 is stuck in the positioning card groove 602, and then release the fixed card seat 603. Under the rebound action of the fixed spring 608, the fixed pressure ring 607 is pushed to slide and reset on the surface of the mounting rod 606. Driven by the connecting protrusion 609, the fixed bracket 603 slides on the surface of the mounting block 6 to cover the surface of the culture dish 7 and the positioning card 701, and the culture dish 7 is clamped and fixed inside the clamping ring 601, which facilitates the combined connection of the culture dish 7 and the clamping ring 601, ensuring that the culture dish 7 will not separate from the clamping ring 601 when the mounting block 6 rotates with the rotating shaft 2.

[0023] As a further solution of the present invention, the mounting mechanism 8 includes a mounting bar 801, the number of the mounting bar 801 is two, the side wall of the incubator 1 is provided with a rectangular groove 101, the mounting bar 801 is fixedly installed on the left and right sides of the inner wall of the rectangular groove 101, and the mounting bars 801 are respectively provided with mounting slots 802 at both ends. A moving block 803 is slidably installed inside the mounting slot 802, and a rubber pad 804 is fixedly installed at one end of the moving block 803, and the rubber pad 804 is tightly attached to the inside of the mounting slot 802; the moving block 803 inside the mounting slot 802 is installed at the top or bottom of the two mounting bars 801. A connecting clip 805 is fixedly mounted on the other end of the mounting strip 801, extending through the mounting slot 802. L-shaped clips 901 are fixedly mounted on the top and bottom of one side of the surface of the illuminator 9, respectively, and the connecting clip 805 is clipped onto the inner side of the L-shaped clip 901. A mounting hole for inserting the movable block 803 is provided on the side wall of the mounting strip 801, which is connected to the mounting slot 802. A connecting through slot is provided at the bottom of the mounting hole, so that after the movable block 803 is inserted into the mounting hole, the connecting portion of the connecting clip 805 and the movable block 803 can slide up and down in the through slot without the movable block 803 being able to escape from the through slot.

[0024] During operation, select the appropriate illuminator 9 according to the needs of cell culture, remove the connecting card strip 805 from the surface of the two mounting strips 801, move the illuminator 9 to correspond to the gap between the two mounting strips 801, push the illuminator 9 into the gap between the two mounting strips 801, and then place the connecting card strip 805 horizontally on one side of the mounting strip 801 so that the moving block 803 corresponds to the mounting slot 802. Insert the moving block 803 into the mounting slot 802 until the rubber pad 804 is aligned with the mounting slot 80 2 inner wall tightly; push the connecting card strip 805 to drive the moving block 803 to slide down inside the installation card slot 802 until the connecting card strip 805 is stuck in the corresponding L-shaped card plate 901, so that the moving block 803 slides down to the bottom inside the installation card slot 802, and the moving block 803 is clamped and fixed in the installation card slot 802 under the rebound action of the rubber clamping pad 804, so that the connecting card strip 805 clamps and fixes the illuminator 9 between the two installation strips 801, which is convenient for disassembly or installation of the illuminator 9.

[0025] As a further solution of the present invention, a second mounting groove 806 is provided at the central position of the mounting bar 801, and a second mounting rod 807 is symmetrically fixedly installed on the upper and lower sides of the second mounting groove 806. A movable top plate 808 is slidably installed on the surface of the second mounting rod 807. A lifting spring 809 is sleeved on the surface of the second mounting rod 807 on the side of the movable top plate 808. A top block 810 is fixedly installed between the two second mounting rods 807, and the top block 810 extends through the second mounting groove 806 to the outside of the mounting bar 801; side plates 902 are symmetrically fixedly installed on both sides of the illuminator 9, and the side plates 902 are overlapped on the surface of the top block 810 corresponding to the position of the second mounting groove 806; During operation, when the illuminator 9 is inserted between the two mounting strips 801, the side panels 902 on both sides of the illuminator 9 are correspondingly inserted into the second mounting groove 806, so that the side panels 902 are close to the top block 810, pushing the top block 810 to slide inside the second mounting groove 806, driving the movable top plate 808 to slide on the surface of the corresponding second mounting rod 807, squeezing the lifting spring 809 to deform on the surface of the second mounting rod 807, and after the connecting clip 805 is pulled out from the inside of the L-shaped clip 901 away from the illuminator 9, the pressure on the illuminator 9 is lost, and the movable top plate 808 is pushed up to slide on the surface of the second mounting rod 807 under the rebound action of the lifting spring 809, so that the top block 810 pushes the side panels 902 out of the second mounting groove 806, making it convenient to pull the illuminator 9 out from between the two mounting strips 801.

[0026] A method for using a modular cell culture container, comprising the following steps: Select the corresponding illuminator 9 according to the light source required for different cell cultures, insert the illuminator 9 between the two mounting bars 801, make the side plates 902 on both sides of the illuminator 9 snap into the corresponding mounting groove 806 and tightly fit with the top block 810, squeeze the top block 810 into the mounting groove 806, drive the movable top plates 808 on both sides of the top block 810 to slide on the surface of the corresponding mounting rod 807, and squeeze the top spring 809 to deform. After the illuminator 9 is completely inserted between the two mounting bars 801, insert the movable blocks 803 at both ends of the connecting clip 805 horizontally into the corresponding mounting slot 802, make the rubber clip 804 tightly fit against the inner wall of the mounting slot 802, slide the connecting clip 805 down and snap into the L-shaped clip 901, so that the illuminator 9 is firmly fixed between the two mounting bars 801; The magnetic door panel 102 is rotated to open, and the fixed card seat 603 is pushed to slide on the surface of the mounting block 6 until the fixed card seat 603 is away from the clamping ring 601. At the same time, the fixed card seat 603 controls the fixed pressure ring 607 to slide on the surface of the mounting rod 1 606 under the drive of the connecting protrusion 609, squeezing the fixed spring 608 to deform, and the culture dish 7 filled with cell culture fluid is clamped into the inside of the clamping ring 601. After the positioning card plate 701 is clamped into the inside of the positioning card groove 602, the fixed card seat 603 is released. Under the rebound action of the fixing spring 608, the fixed pressure ring 607 is lifted up to slide back on the surface of the mounting rod 1 606 until the fixed card seat 603 is moved and clamped on the top of the culture dish 7, and the culture dish 7 is clamped and fixed inside the clamping ring 601. Repeat the steps to clamp multiple culture dishes 7 into the corresponding clamping rings 601 respectively; The magnetic door panel 102 is rotated to fit tightly against the incubator 1, squeezing the sealing strip 103 to deform, so that the interior of the incubator 1 is in a relatively closed environment, controlling the illuminator 9 to emit light suitable for culturing cells inside the culture dish 7, and at the same time controlling the rotating motor 4 to drive the bevel gear 2 5 to rotate. Under the meshing action of the bevel gear 2 5 and the bevel gear 1 3, the rotating shaft 2 rotates inside the incubator 1, driving the multiple mounting blocks 6 and the culture dishes 7 to rotate synchronously at a uniform speed inside the incubator 1, ensuring that the culture dishes 7 pass through the illuminator 9 at a uniform speed, so that the culture dishes 7 at different positions on the surface of the rotating shaft 2 are evenly illuminated by the light emitted by the illuminator 9, thereby preventing the culture dishes 7 at different positions inside the incubator 1 from being exposed to light of different intensities and durations, resulting in large deviations in the culture of the culture dishes 7; When disassembling and replacing the illuminator 9, lift the connecting clip 805 away from the L-shaped card plate 901. After the two connecting clips 805 are away from the L-shaped card plate 901, the thrust on the illuminator 9 is cancelled, and the second mounting rod 807 is lifted under the rebound action of the lifting spring 809, so that the lifting block 810 pushes the side plate 902 out of the second mounting groove 806, thereby pushing the illuminator 9 out from the two mounting strips 801 brackets, making it convenient to disassemble and replace the illuminator 9, and replace the illuminator 9 with different light according to different cell culture lighting conditions.

Claims

1. A modular cell culture container, comprising an incubator (1), characterized in that: A rotating shaft (2) is rotatably mounted between the top and bottom of the inner side of the incubator (1) via a bearing, the top end of the rotating shaft (2) passes through the incubator (1) and is fixedly mounted with a bevel gear 1 (3), a rotating motor (4) is fixedly mounted on one side of the top of the incubator (1), a bevel gear 2 (5) is fixedly mounted on the output end of the rotating motor (4), and the bevel gear 2 (5) is meshed with the bevel gear 1 (3), a plurality of mounting blocks (6) are fixedly mounted on the surface of the rotating shaft (2) located on the inner side of the incubator (1), a culture dish (7) is clamped on one end of the mounting block (6) away from the rotating shaft (2), a mounting mechanism (8) is fixedly mounted at the central position of one side of the incubator (1), and an illuminator (9) is clamped inside the mounting mechanism (8).

2. The modular cell culture container according to claim 1, characterized in that: A rectangular groove (101) is provided on one side of the incubator (1), and a magnetic door panel (102) is rotatably mounted on one side of the opening of the incubator (1) via a pin shaft, and a sealing strip (103) is fixedly mounted on the surface of the magnetic door panel (102).

3. The modular cell culture container according to claim 1, characterized in that: A clamping ring (601) is symmetrically fixedly installed on one end of the mounting block (6) away from the rotating shaft (2), a positioning slot (602) is provided at the top center position of the mounting block (6) near the clamping ring (601), a fixed clamping seat (603) is slidably installed through the surface of the mounting block (6), mounting slots (604) are symmetrically provided on both sides of the mounting block (6), and sliding slots (605) are provided on both sides of the mounting block (6) near the clamping ring (601), and the sliding slots (605) are communicated with the mounting slot (604).

4. The modular cell culture container according to claim 3, characterized in that: The inner wall of the mounting groove (604) is fixedly mounted with a mounting rod (606), the surface of the mounting rod (606) is slidably mounted with a fixed pressure ring (607), the surface of the mounting rod (606) on one side of the fixed pressure ring (607) is sleeved with a fixed spring (608), the surface of the fixed pressure ring (607) is fixedly mounted with a connecting protrusion (609), and the connecting protrusion (609) is slidably connected to the inside of the slide groove (605), and the end of the connecting protrusion (609) away from the fixed pressure ring (607) is fixedly connected to the inner wall of the fixed clamping seat (603).

5. The modular cell culture container according to claim 4, characterized in that: A positioning card plate (701) is fixedly mounted on the top of the surface of the culture dish (7), and the positioning card plate (701) is clamped inside the positioning card slot (602). A buckle slot (702) is provided on one side of the positioning card plate (701).

6. The modular cell culture container according to claim 2, characterized in that: The mounting mechanism (8) comprises a mounting bar (801), wherein the number of the mounting bars (801) is two, and the mounting bars (801) are fixedly mounted on both sides of the inner wall of the rectangular groove (101), and mounting slots (802) are respectively provided at both ends of the mounting bar (801), and a moving block (803) is slidably mounted inside the mounting slot (802), and a rubber card pad (804) is fixedly mounted on one side of the moving block (803), and the rubber card pad (804) is tightly attached to the inside of the mounting slot (802), and the moving block (803) on the inner side of the mounting slot (802) at the top or bottom of the two mounting bars (801) passes through the mounting slot (802) and extends to the outer side of the mounting bar (801) where a connecting card strip (805) is fixedly mounted.

7. The modular cell culture container according to claim 6, characterized in that: A second mounting groove (806) is provided at the central position of the mounting bar (801), and two mounting rods (807) are symmetrically fixedly installed on both sides of the inside of the second mounting groove (806), and a movable top plate (808) is slidably installed through the surface of the second mounting rod (807), and a lifting spring (809) is sleeved on the surface of the second mounting rod (807) located on one side of the movable top plate (808), and a top block (810) is fixedly installed between the two second mounting rods (807), and the top block (810) passes through the second mounting groove (806) and extends to the outside of the mounting bar (801).

8. The modular cell culture container according to claim 7, characterized in that: An L-shaped card plate (901) is fixedly mounted on the top and bottom of one side of the surface of the illuminator (9), and a connecting card strip (805) is clamped on the inner side of the L-shaped card plate (901). Side plates (902) are symmetrically fixedly mounted on both sides of the illuminator (9), and the side plates (902) are overlapped on the surface of the top block (810) corresponding to the position of the second mounting groove (806).

9. A method for using a modular cell culture container, according to any one of claims 1 to 8, characterized in that: The method of use comprises the following steps: Select the corresponding illuminator (9) according to the light source required for different cell cultures, insert the illuminator (9) between the two mounting bars (801), make the side plates (902) on both sides of the illuminator (9) snap into the corresponding mounting groove 2 (806) and close to the top block (810), squeeze the top block (810) into the mounting groove 2 (806), and drive the movable top plates (808) on both sides of the top block (810) to slide and squeeze on the surface of the corresponding mounting rod 2 (807). After the jacking spring (809) is deformed and the illuminator (9) is completely inserted between the two mounting strips (801), the movable blocks (803) at both ends of the connecting strip (805) are horizontally inserted into the corresponding mounting slots (802), so that the rubber pad (804) is tightly attached to the inner wall of the mounting slot (802), and the connecting strip (805) is lowered and inserted into the L-shaped card plate (901), thereby firmly fixing the illuminator (9) between the two mounting strips (801); The magnetic door panel (102) is rotated to open, and the fixed card seat (603) is pushed to slide on the surface of the mounting block (6) until the fixed card seat (603) is away from the clamping ring (601). At the same time, the fixed card seat (603) is driven by the connecting protrusion (609) to control the fixed pressure ring (607) to slide on the surface of the mounting rod (606), squeezing the fixed spring (608) to deform, and the culture dish (7) filled with cell culture fluid is clamped into the inside of the clamping ring (601), so that the positioning After the card plate (701) is inserted into the positioning card slot (602), the fixed card seat (603) is released and the fixed pressure ring (607) is lifted up under the rebound action of the fixed spring (608) and slides back on the surface of the mounting rod (606) until the fixed card seat (603) is moved and clamped on the top of the culture dish (7), and the culture dish (7) is clamped and fixed inside the clamping ring (601). Repeat the steps to clamp multiple culture dishes (7) into the corresponding clamping rings (601). The magnetic door panel (102) is rotated to be close to the incubator (1), and the sealing strip (103) is squeezed and deformed, so that the interior of the incubator (1) is in a relatively closed environment, and the illuminator (9) is controlled to emit light suitable for culturing cells inside the culture dish (7). At the same time, the rotating motor (4) is controlled to drive the bevel gear 2 (5) to rotate. Under the meshing action of the bevel gear 2 (5) and the bevel gear 1 (3), the rotating shaft (2) is rotated inside the incubator (1), driving the multiple mounting blocks (6) and the culture dish (7) to rotate synchronously at a uniform speed inside the incubator (1), ensuring that the culture dish (7) passes the illuminator (9) at a uniform speed, so that the culture dishes (7) at different positions on the surface of the rotating shaft (2) are evenly illuminated by the light emitted by the illuminator (9), and it is avoided that the culture dishes (7) at different positions inside the incubator (1) are exposed to light of different intensities and durations, resulting in a large deviation in the culture of the culture dishes (7); When the illuminator (9) is disassembled and replaced, the connecting card strip (805) is lifted away from the L-shaped card plate (901). After the two connecting card strips (805) are away from the L-shaped card plate (901), the thrust on the illuminator (9) is canceled, and the second mounting rod (807) is lifted under the rebound action of the lifting spring (809), so that the lifting block (810) pushes the side plate (902) out of the second mounting groove (806), thereby pushing the illuminator (9) out of the two mounting strip (801) brackets, making it convenient to disassemble and replace the illuminator (9), and replace the illuminator (9) with different light according to different cell culture illumination conditions.

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