Washing and cleaning agents comprising dispersants

Abstract

The present invention relates to washing and cleaning reagents, in particular enzyme-containing washing and cleaning reagents. The invention relates to the use of dispersants or dispersants-containing agents for preventing and / or reducing undesirable odors in surfaces, in particular household surfaces, preferably washing machines or dishwashers, pump pools, storage tanks, reservoirs, washing drums, rinse rooms, washbasins or sinks, toilets, water tanks, toilet rims, siphons, water and / or lines, water inlets and / or water outlets, and / or for preventing and / or reducing and / or removing microbial-induced deposits in surfaces, in particular household surfaces, preferably washing machines or dishwashers, pump pools, storage tanks, reservoirs, washing drums, rinse rooms, washbasins or sinks, toilets, water tanks, toilet rims, siphons, water and / or lines, water inlets and / or water outlets, and / or for improving the hygiene in surfaces, in particular household surfaces, preferably washing machines or dishwashers, pump pools, storage tanks, reservoirs, washing drums, rinse rooms, washbasins or sinks, toilets, water tanks, toilet rims, siphons, water and / or lines, water inlets and / or water outlets.

Description

[0001] The present invention relates to washing and cleaning agents, in particular washing and cleaning agents containing enzymes. The present invention relates to the use of dispersin or dispersin-containing agents for preventing and / or reducing odors on surfaces, in particular household surfaces, preferably washing machines or dishwashers, pump sump, storage tanks, water reservoirs, washing drums, rinse chambers, wash basins or sinks, toilets, water tanks, toilet rims, siphons, water pipes and / or pipelines, water inlets and / or water outlets; and / or for preventing and / or reducing and / or removing odors on surfaces, in particular household surfaces, preferably washing machines or dishwashers, pump sump, storage tanks, water reservoirs The invention also relates to a method for improving the hygiene of surfaces, in particular domestic surfaces, preferably washing machines or dishwashers, pump sump, tanks, reservoirs, washing drums, rinse chambers, washbasins or sinks, toilets, water tanks, toilet rims, siphons, water pipes and / or lines, water inlets and / or water outlets. The invention also relates to corresponding agents and methods.

[0002] In the prior art, the use of enzymes in detergents has been around for decades. They are used to expand the performance range of related reagents based on their specific activity. These enzymes include, in particular, hydrolases, such as proteases, amylases, lipases, and cellulases. The first three enzymes mentioned hydrolyze proteins, starches, and fats, thereby directly contributing to the removal of dirt. Cellulases are used in textile detergents, particularly due to their effect on fabrics. Another group of detergent enzymes is oxidases, particularly oxidases, which are optionally combined with other components and are preferably used for bleaching stains and / or producing bleaching agents in situ. In addition to these continuously optimized enzymes, other enzymes such as pectinases, pectate lyases, xanthanases, beta-glucanases, mannanases, dispersins, etc. continue to be utilized in detergents, particularly for optimally handling specific stains, particularly for the hydrolysis of specific plant polymers. Typically, only selected enzymes are suitable for use in formulations containing liquid surfactants. Many enzymes do not exhibit sufficient catalytic performance in such formulations. Therefore, for the use of enzymes in detergents, high catalytic activity and high storage stability under the conditions during the wash cycle are particularly desirable.

[0003] The form of manufacture and supply of washing and cleaning agents is put forward to changing requirements. For quite a long time, the main focus has been on the convenient dosing (dosing) of washing and cleaning agents by consumers, and on simplifying the work steps required for performing washing or cleaning methods. A technical solution is provided by pre-distributing washing or cleaning agents (for example, film pouches comprising one or more holding chambers for solid and / or liquid washing and cleaning agents). The trend associated with the production of these film pouches is the miniaturization of these film pouches. In addition to obtaining higher consumer acceptance due to simplified operation, the background of this development is especially sustainability, for example, with regard to the quantity of transport volume and cost and the packaging materials used. The focus of modern washing and cleaning agents assumes the gradual optimization of existing formulations and the development of novel, more efficient formulations. One goal of these developments (wherein known active washing and cleaning ingredients are generally used) is to achieve an improvement in the washing or cleaning performance of the formulation when the dosing amount is constant or reduced.

[0004] The most important criterion when cleaning textiles and / or hard surfaces is cleaning performance on a wide variety of stains. Although the cleaning performance of today's washing and cleaning agents is generally high, the general trend towards increasing the use of low-temperature programs and the use of compressed formulations (especially in pre-dispensed form) has led to the problem of insufficient cleaning performance of many conventional washing and cleaning agent formulations, especially on stubborn stains. This insufficient cleaning performance leads to consumer dissatisfaction and leads consumers to pre-treat such stains, which in turn increases water and energy consumption.

[0005] The term "odor" as used herein refers to undesirable odors on surfaces, in particular household surfaces, tableware, textiles and cleaned items, in particular cleaned textiles and cleaned tableware. Cleaned items should smell fresh and clean, without odors clinging to them. Various reasons can lead to unpleasant odors (called odors) on surfaces, in particular household surfaces, as well as on washed textiles and / or cleaned hard surfaces (in particular tableware). The term "household surfaces" as used herein refers to surfaces such as washing machines or dishwashers, pump sump, storage tank, reservoir, washing drum, rinse chamber, wash basin or sink, toilet, water tank, toilet rim, siphon, water pipe and / or pipeline, water inlet and / or water outlet. Odors are a known problem, in particular for textiles and washing machines. However, odors can also occur in dishwashers. It can affect all parts that come into contact with dirty items and / or water (especially used water) and / or washing / cleaning liquids, such as washing machines or dishwashers, pump sump, storage tanks, reservoirs, washing drums, rinse chambers, wash basins or sinks, toilets, water tanks, toilet rims, siphons, water pipes and / or pipelines, water inlets and / or outlets. One (if not the most common) cause of this is substances of an organic nature, such as sebum, body dirt (e.g., dead skin cells and sweat), cell residues, food residues, body excretions, biofilms and extracellular polymeric substances (EPS; e.g., extracellular polymeric sugars). It should also be emphasized here that microbial bio-buildup, i.e., microbial odors that are the result of microbial bio-buildup. Another example of an unpleasant odor is sweat or body odor that adheres to items that have come into contact with people or animals. Odors are present not only in washing machines, but also on freshly washed, still damp laundry, as well as on dried laundry, and on numerous other surfaces, in particular household surfaces that have come into contact with soiled items and / or water (in particular used water) and / or washing / cleaning soap, such as surfaces in washing machines or dishwashers, pump sump, tanks, reservoirs, washing drums, rinse chambers, washing or dishwashing sinks, toilets, toilet tanks, etc., such as surfaces in washing machines or dishwashers, pump sump, tanks, reservoirs, washing drums, rinse chambers, washbasins or sinks, toilets, tanks, toilet rims, siphons, water pipes and / or lines, water inlets and / or water outlets. However, deposits, bioaccumulations, body soils, etc. not only cause odors but can also appear as stains on textiles and / or surfaces, in particular on household surfaces, such as surfaces in washing machines or dishwashers, pump sump, tanks, cisterns, washing drums, rinse chambers, wash basins or sinks, toilets, water tanks, toilet rims, siphons, pipes and / or lines, water inlets and / or outlets, for example in the form of coatings, stains, encrustations, yellowing, underarm stains, and graying. Due to their typically sticky texture, bioaccumulations, etc., also allow other odor and / or dirt particles to adhere.However, odor may already be present before microbially induced deposits lead to visible soiling.

[0006] The term "bioaccumulation" as used herein refers not only to biofilms in the strict sense (e.g., microbially induced deposits, biofilms, etc., which can be formed by any microorganism, whose cells adhere to each other and / or adhere to surfaces such as textiles, cookware or hard surfaces or other types of surfaces), but also more generally to any bioaccumulation, biofouling, bioinduced deposits, etc., including but not limited to the degradation and deposition products of microorganisms that adhere to surfaces (e.g., textiles, cookware or hard surfaces or other types of surfaces). These adherent cells are typically embedded in a matrix of extracellular polymeric substances (EPS) produced by themselves. Biofilm EPS is a polymer aggregate that typically contains extracellular DNA, proteins, and polysaccharides. Biofilms can be formed on living or inanimate surfaces. Microbial cells grown in biofilms are physiologically different from planktonic cells of the same organism, which are single cells that float or can float in a liquid medium. Bacterial cells living in biofilms typically have significantly different properties from planktonic bacterial cells of the same species because the dense and protected environment of the membrane allows them to cooperate and interact in different ways. One advantage of this medium for microorganisms is the enhanced resistance to detergents and antibiotics because the dense extracellular matrix and outer layer of cells protect the interior of the community. Biofilm-producing bacteria on textiles include, for example, the following species: Acinetobacter sp., Aeromicrobium sp., Brevundimonas sp., Microbacterium sp., Micrococcus luteus, Pseudomonas sp., Staphylococcus epidermidis, and Stenotrophomonas sp. On hard surfaces, biofilm-forming bacteria can be found, such as the following species: Acinetobacter sp., Aeromicrobium sp., Brevundimonas sp., Microbacterium sp., Micrococcus luteus, Pseudomonas sp., Staphylococcus epidermidis, Staphylococcus aureus, and Stenotrophomonas sp.

[0007] EP 3892708, WO 2021 / 122117, WO 2021 / 122118, WO 2021 / 122120, WO 2021 / 122121 and WO 2022 / 008387 describe washing and cleaning agents containing dispersin and enzymes. For example, dispersin has also been described to degrade biofilms and EPS.

[0008] Accumulated organic soils are often difficult to remove, and commercially available washing and cleaning agents do not adequately reduce odors. Instead, odors are masked by the use of various fragrances, which are often perceived by consumers as a cleaning effect, even though the root cause of the odor is not actually eliminated. As a result, more and more organic matter, and therefore more and more odors, accumulate over time, and more and more fragrances must be added to prevent textiles from smelling worse. However, many fragrances have side effects, such as allergenicity, which can manifest in the form of rashes. Furthermore, many of these substances are natural chemicals and are harmful to the environment if they enter wastewater. In recent years, fragrances have been increasingly used in the form of fragrance microcapsules, despite the fact that microcapsules are known to be microplastics and therefore potentially harmful to the environment. Fragrances are also expensive and increase the overall cost of washing and cleaning products.

[0009] Therefore, there is a need to further improve the cleaning performance of enzyme-containing detergents and / or cleaning agents (especially pre-dispensed textile detergents), especially on stubborn stains. There is also a need to improve the removal of odors in washing machines and / or dishwashers and / or washed textiles or cleaned surfaces (especially dishes) and on surfaces (especially domestic surfaces such as washing machines or dishwashers, pump sump, tanks, reservoirs, washing drums, rinse chambers, washbasins or sinks, toilets, water tanks, toilet rims, siphons, water pipes and / or lines, water inlets and / or water outlets). There is also a need to improve the hygiene of washing machines and dishwashers and to reliably remove bioaccumulations caused by microorganisms from surfaces (especially domestic surfaces such as washing machines or dishwashers, pump sump, tanks, reservoirs, washing drums, rinse chambers, washbasins or sinks, toilets, water tanks, toilet rims, siphons, water pipes and / or lines, water inlets and / or water outlets). This also includes (semi-)automated washing or cleaning systems such as mopping robots or wet vacuum cleaners.

[0010] Surprisingly, it has now been found that the dispersants according to the invention reliably remove microbiologically caused odors as well as microbially induced deposits, bioaccumulations, etc. from surfaces, in particular household surfaces (such as surfaces in washing machines or dishwashers, pump sump, storage tanks, water reservoirs, washing drums, rinse chambers, washbasins or sinks, toilets, water tanks, toilet rims, siphons, water pipes and / or lines, water inlets and / or water outlets).

[0011] Thus, in a first aspect, a subject of the present invention is a dispersin having β-1,6-N-acetylglucosaminidase activity, comprising an amino acid sequence that is at least 70% (and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identical over its entire length to the amino acid sequence given in SEQ ID NO: 1, and based on the method according to SEQ ID NO: 2. NO:1 number, in the same position as position 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71, 72, 74, 77, 79, 80, 81, 82, 90, 99, 100, 103, 104, 105, 106, 107, 110, 111 、113、114、116、117、118、119、120、122、123、124、125、126、127、128、131、135、138、139、140、142、145、147、148、149、150、151、152、163、164、167、168、170、171、173、174、175、177、178、179、181、 185, 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221, 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251, 252, 253, 254, 256, 260, 261, 262, 263, 264, 265, 2 67, 268, 270, 271, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321 and 323 have at least one amino acid substitution and / or deletion:

[0012] For preventing and / or reducing odors on surfaces, in particular domestic surfaces, preferably washing machines or dishwashers, pump sump, tanks, cisterns, washing drums, rinse chambers, washbasins or sinks, toilets, cisterns, toilet rims, siphons, water pipes and / or lines, water inlets and / or water outlets; and / or

[0013] For preventing and / or reducing and / or removing microbiologically induced deposits on surfaces, in particular on domestic surfaces, preferably washing machines or dishwashers, pump sump, tanks, cisterns, washing drums, rinse chambers, washbasins or sinks, toilets, cisterns, toilet rims, siphons, water pipes and / or lines, water inlets and / or water outlets; and / or

[0014] For improving hygiene on surfaces, in particular domestic surfaces, preferably in washing machines or dishwashers, pump sump, tanks, reservoirs, washing drums, rinse chambers, washbasins or sinks, toilets, cisterns, toilet rims, siphons, water pipes and / or lines, water inlets and / or water outlets.

[0015] In another aspect, the present invention relates to the use of a dispersin having β-1,6-N-acetylglucosaminidase activity, comprising an amino acid sequence that is at least 70% (and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identical over its entire length to the amino acid sequence given in SEQ ID NO: 1, and based on the method according to SEQ ID NO: 104, 105, 106, 107, 110, 111, 113, 114, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151 2, 123, 124, 125, 126, 127, 128, 131, 135, 138, 139, 140, 142, 145, 147, 148, 149, 150, 151, 152, 163, 164, 167, 168, 170, 171, 173, 174, 175, 177, 178, 179, 181, 185, 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218 , 221, 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251, 252, 253, 254, 256, 260, 261, 262, 263, 264, 265, 267, 268, 270, 271, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304 , 305, 306, 308, 309, 312, 314, 315, 319, 321 and 323 have at least one amino acid substitution and / or deletion selected from the group consisting of: D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19N,E19P、K22A、K22M、K22V、S23C、S23E、S23I、S23L、S23R、S23T、S23V、V25R、D26M、Y30*、Y30D、Y30L、Y30M、Y30N、Y30R、Y30T、Y30V、G32L、G32M、G32R、N43*、N43H、N43L、E44*、N45D、N45L、N45V、A49W、A49Y、Y52*、Y52M、G54L、G54M、G54N、S56T、S56W、S57W、E58N、N59A、N59C、N59D、N59E、N59F、N59M、N59R、N59V、N59W、T60V、N62C、N62D、N62H、N62Q、N62W、T63C、T63D、T63L、T63N、T63R、T63V、K67A、K67L、N68L、N68Q、L71H、L71N、L71R、L71V、L71W、S72*、S72C、S72D、S72E、S72F、S72G、S72I、S72M、S72N、S72R、S72T、S72Y、I74L、S77A、D79V、K80*、K80E、K80H、K80L、K80N、K80Q、K80V、K80W、D81A、D81G、D81L、D81R、D81S、D81T、D81V、D81W、I82V、L90F、E99Q、E99R、L100S、K103A、K103R、K104N、K104W、D105N、V106A、V106D、V106E、V106H、V106K、V106L、V106M、V106N、V106Q、V106W、V106Y、K107A、K107C、K107L、K107M、K107T、K107V、K107W、N110M、N110R、N110V、D111A、D111E、D111M、D111N、D111Q、D111R、D111V、D111W、V113T、T114C、T114S、Y116D、Y116N、Y116R、S117*、S117D、S117H、S117N、S117P、E118*、E118A、E118D、E118G、E118L、E119G、E119W、T120I、T120L、T120M、T120V、T120W、D122*、D122H、D122R、Y123W、Y124C、Y124I、Y124K、Y124L、Y124M、Y124Q、Y124R、Y124T、Y124V、Y124W、D125C、D125G、D125K、D125Q、D125R、N126V、R127D、R127H、R127K、R127L、R127M、R127Q、R127W、V128C、V128L、V128T、D131V、Q135*、Q135A、Q135D、Q135E、Q135K、Q135M、Q135Y、D138K、D138L、D138M、D138Q、D138R、D138S、D138V、D138W、E139W、D142R、D142W、Y145*、Y145H、Y145L、Y145N、Y145V、P147A、P147C、P147D、P147F、P147G、P147L、P147M、P147R、P147S、P147T、P147V、K148A、K148D、K148L、K148V、F149L、F149M、F149N、E150D、E150H、E150K、E150L、E150M、E150N、E150R、E150V、E150W、E150Y、G151A、G151C、G151D、G151L、G151N、G151P、G151S、G151W、K152D、K152L、K152R、G164D、G164E、G164H、G164S、G164V、V167D、V167E、V167L、V167P、V167Q、V167R、V167W、H168N、L170A、L170D、L170E、L170F、L170H、L170K、L170M、L170N、L170P、L170Q、L170R、L170S、L170V、L170W、L170Y、D171A、D171C、D171E、D171K、D171L、D171M、D171Q、D171R、D171V、D171W、D171Y、I173C、D174H、D174M、D174N、D174V、D174W、F175Y、N177M、Q178*、Q178A、Q178K、Q178R、Q178W、I179T、S181C、S181D、S181F、S181G、S181N、S181P、S181Q、S181T、S181V、S181W、E185M、E185R、E185V、E185W、S186D、S186E、S186H、S186I、S186K、S186L、S186M、S186N、S186Q、S186R、S186V、S186W、K187C、K187D、K187G、K187R、K187S、K187V、K187W、Y188P、E189L、E189V、E189W、S199C、S199L、S199M、S199Y、E200D、E200F、E200K、E200L、E200M、E200N、E200R、E200W、A203C、A203D、A203E、A203G、A203L、A203M、A203P、A203R、A203S、A203T、A203V、A203W、N204L、N204M、N204V、N204W、N204Y、L205I、D207A、D207C、D207E、D207G、D207N、D207Q、D207S、D207V、D207W、S208A、S208C、S208D、S208G、S208L、S208Q、S208T、S208V、S208W、S210T、Q215R、Q215M、Q215L、Q215*、S217V、T218A、T218L、T218Q、T218R、T218V、G222D、E224A、E224P、N227A、N227Q、N227R、N227S、N227T、N227K、D230*、D230R、D230T、D230W、E232D、E232V、N233H、N233Q、N233R、N233W、W234R、G235W、G235A、G235E、G235F、G235H、G235I、G235L、G235M、G235N、G235P、G235S、G235V、S237C、S237G、S237M、S237N、S237W、S237Y、Y244C、Y244E、Y244M、L249H、L249K、L249Q、L249R、L249W、L249Y、S251L、S251N、S251R、S251W、N252P、N252C、G253D、G253W、F254I、F254L、F254M、F254Y、Q256E、Q256R、N260*、N260A、N260C、N260E、N260I、N260K、N260L、N260M、N260Q、N260R、N260V、N260W、N260Y、E261*、E261A、E261D、E261R、E261W、Q262*、Q262F、Q262H、Q262W、Q262Y、M263K、M263L、M263Q、D264*、D264C、D264E、Y265F、N267S、N267T、W268C、W268E、W268M、W268R、Y270F、A271D、A271G、H272D、H272I、Y281P、Y282E、Y282N、Y281R、H272W、N273W、K274R、K274A, K274H, F276A, F276C, F276K, F276N, F276G, F276L, F276M, F276P, F276S, F276V, F276W , I278A, I278K, I278N, I278Q, I278V, S279C, S279D, S279E, S279G, S279N, D280C, D280E, Y281 *, Y281A, Y281C, Y281H, Y281K, Y281N, Y281P, Y282E, Y282N, H283I, A284I, A284L, A284N, A28 4P, A284T, A284V, T287N, S288D, S288K, S288N, V290I, K291L, K291R, K291V, T296C, E300A, E3 00D, H301C, H301N, H301R, T303A, T303C, T303G, T303K, T303Q, T303R, T303W, D304C, D304M, L 305M, L305N, S306C, K308A, K308D, K308G, K308I, K308L, K308Q, K308S, K308T, K308V, K308Y, K309A, K309C, K309D, K309H, K309L, K309M, K309N, K309Q, K309S, K309T, K309I, K312A, K312L, K312M, K312N, K312Q, K312S, K312W, E314I, E314L, E314V, L315I, L315V, R319A, N323R, and combinations thereof,

[0016] for preventing and / or reducing odours on surfaces, in particular domestic surfaces, preferably washing machines or dishwashers, pump sump, tanks, cisterns, washing drums, rinse chambers, washbasins or sinks, toilets, cisterns, toilet rims, siphons, water pipes and / or lines, water inlets and / or water outlets; and / or

[0017] for preventing and / or reducing and / or removing microbiologically induced deposits on surfaces, in particular domestic surfaces, preferably in washing machines or dishwashers, pump sump, tanks, cisterns, washing drums, rinse chambers, washbasins or sinks, toilets, cisterns, toilet rims, siphons, water pipes and / or lines, water inlets and / or water outlets; and / or

[0018] For improving hygiene on surfaces, in particular domestic surfaces, preferably in washing machines or dishwashers, pump sump, tanks, reservoirs, washing drums, rinse chambers, washbasins or sinks, toilets, cisterns, toilet rims, siphons, water pipes and / or lines, water inlets and / or water outlets.

[0019] In a preferred embodiment, the dispersant is contained in a washing agent and / or a cleaning agent, wherein the agent is substantially free of boron-containing compounds, preferably free of boron-containing compounds; and / or the pH of the agent in a 1 wt % solution in deionized water at 20° C. is in the range of about 6 to about 11, in particular in the range of about 6.5 to about 10.5, more preferably in the range of about 7 to about 10, and particularly preferably in the range of about 8 to about 9.

[0020] In a further preferred embodiment, the dispersin or the washing agent and / or cleaning agent containing dispersin is used in a temperature range of about 10°C to about 60°C, preferably about 15°C to about 40°C, particularly preferably 20°C to about 30°C.

[0021] A further subject matter of the present invention relates to an agent for cleaning surfaces (in particular domestic surfaces, preferably washing machines or dishwashers, pump sumps, tanks, reservoirs, washing drums, rinse chambers, washbasins or sinks, toilets, water tanks, toilet rims, siphons, water pipes and / or lines, water inlets and / or water outlets), in particular for preventing and / or reducing odors and / or for preventing and / or reducing and / or removing microbiologically induced deposits, and / or for improving hygiene, characterized in that the agent contains a dispersant according to the invention; and an agent for cleaning surfaces (in particular domestic surfaces, preferably washing machines or dishwashers, pump sumps, tanks, reservoirs, washing drums, rinse chambers, washbasins or sinks, toilets, water tanks, toilet rims, siphons, water pipes and / or lines, water inlets and / or water outlets). , tanks, reservoirs, washing drums, rinse chambers, washbasins or sinks, toilets, cistern, toilet rims, siphons, water pipes and / or lines, water inlets and / or water outlets), in particular for preventing and / or reducing odours and / or for preventing and / or reducing and / or removing microbiologically induced deposits and / or for improving hygiene, characterized in that a dispersant according to the invention or an agent according to the invention is used in at least one method step, preferably in a temperature range of about 10° C. to about 60° C., preferably about 15° C. to about 40° C., particularly preferably about 20° C. to about 30° C.

[0022] These and other aspects, features, and advantages of the present invention will become apparent to those skilled in the art upon study of the following detailed description and claims. Any feature in one aspect of the present invention may be used in any other aspect of the present invention. Furthermore, it will be readily understood that the examples contained herein are intended to illustrate and explain, not to limit, the present invention, and in particular, the present invention is not limited to these examples.

[0023] Unless otherwise indicated, all percentages are expressed as weight percent (wt %).

[0024] Numerical ranges expressed in the format "from x to y" also include the stated values. If several preferred numerical ranges are specified in this format, it is readily understood that any range resulting from a combination of the various endpoints is also included.

[0025] As used herein, "at least one" means one or more, i.e., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14 or more.

[0026] The terms "washing and / or cleaning agent", "washing and cleaning agent" or "washing or cleaning agent" as used herein are synonymous with the term "agent" and, as described in the specification, denote compositions for cleaning textiles and / or hard surfaces, in particular dishes.

[0027] As used herein, "about," "approximately," or "substantially" when referring to a numerical value refers to ±10%, preferably ±5%, of the corresponding numerical value.

[0028] As used herein, "liquid" includes liquids and gels as well as pasty compositions.Preferred liquid compositions are flowable and pourable at room temperature, but they may also have flowability limits.

[0029] According to the definition of the present invention, a substance (eg a composition or an agent) is a solid if it is in the solid state of matter at 25° C. and 10 13 mbar.

[0030] According to the definition of the present invention, a substance (eg a composition or an agent) is a liquid if it is in the liquid state of matter at 25° C. and 1013 mbar. Liquid also includes gel forms.

[0031] As used herein, "variant" refers to a naturally occurring or artificially produced variant of a native enzyme having a modified amino acid sequence relative to a reference form.

[0032] The term "deep cleaning" as used herein means dissolving or removing visible and invisible components of organic matter, such as animal fat, cell residues, body dirt, sebum, bioaccumulations, biofilms (including, for example, polysaccharides, PNAG (poly-N-acetylglucosamine)), body oils or other components present in organic matter, not only at a level visible to the naked eye, but also includes dissolving or removing microbial-induced deposits that are invisible to the naked eye, especially deposits between individual textile fibers, bioaccumulations, etc.

[0033] Term used herein " textile " means any textile material, comprises yarn, yarn precursor, fiber, nonwoven, natural material, synthetic material and all other textile materials, the fabric made of these materials and the product (for example clothing and other articles) made of fabric.Textile or fabric can be the form of knitted fabric, woven fabric, denim, nonwoven, felt, yarn and terry cloth.Textile can be based on cellulose, for example natural cellulose fiber, as cotton, flax / linen, jute, ramie, sisal or coconut fiber, or the cellulose fiber (for example from paper pulp) of synthetic production, as viscose / rayon, cellulose acetate fiber (tricell), lyocell or its mixture.Textile or fabric also can be made up of non-cellulose fiber, for example natural polyamide, as wool, camel hair, cashmere, mohair, angora and silk, or synthetic polymer, as nylon, aramid, polyester, acrylic acid, polypropylene and spandex / elastic fiber or its mixture, and the mixture of cellulose fiber and non-cellulose fiber. Examples of blends are blends of cotton and / or rayon / viscose with one or more accompanying materials such as wool, synthetic fibers (e.g. polyamide fibers, acrylic fibers, polyester fibers, polyvinyl chloride fibers, polyurethane fibers, polyurea fibers, aramid fibers) and / or cellulosic fibers (e.g. rayon / viscose, ramie, flax / linen, jute, cellulose acetate fibers, lyocell fibers). The fabric may be conventional washable clothing, such as soiled household clothing. When the term "fabric" or "garment" is used, it is intended to include the broader term "textile".

[0034] The present invention is based on the surprising discovery of the inventors that the dispersants according to the invention reliably remove microbiologically caused odors as well as microbially induced deposits, bioaccumulations, etc. from surfaces (in particular household surfaces, such as surfaces in washing machines or dishwashers, pump sump, storage tanks, water reservoirs, washing drums, rinse chambers, washbasins or sinks, toilets, water tanks, toilet rims, siphons, water pipes and / or lines, water inlets and / or water outlets).

[0035] According to the present invention, a dispersin having β-1,6-N-acetylglucosaminidase activity is used for the following uses, and the dispersin comprises an amino acid sequence that is at least 70% (and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identical to the amino acid sequence given in SEQ ID NO: 1 over the entire length, and is based on the amino acid sequence given in SEQ ID NO: 1. 104, 105, 106, 107, 110, 111, 113, 114, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155 9, 120, 122, 123, 124, 125, 126, 127, 128, 131, 135, 138, 139, 140, 142, 145, 147, 148, 149, 150, 151, 152, 163, 164, 167, 168, 170, 171, 173, 174, 175, 177, 178, 179, 181, 185, 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208 8, 210, 215, 217, 218, 221, 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251, 252, 253, 254, 256, 260, 261, 262, 263, 264, 265, 267, 268, 270, 271, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 29 0, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321 and 323 have at least one amino acid substitution and / or deletion: prevent and / or reduce the surface (especially household surfaces, preferably washing machines or dishwashers, pump tanks, storage tanks, reservoirs, washing drums, rinse chambers, washbasins or sinks, toilets, water tanks, toilet rims, siphons, pipes and / or pipelines, water inlets and / or outlets);and / or prevent and / or reduce and / or remove microbiologically induced deposits on surfaces, in particular domestic surfaces, preferably in washing machines or dishwashers, pump sumps, tanks, reservoirs, washing drums, rinse chambers, washbasins or sinks, toilets, cisterns, toilet rims, siphons, water pipes and / or lines, water inlets and / or water outlets; and / or improve hygiene on surfaces, in particular domestic surfaces, preferably in washing machines or dishwashers, pump sumps, tanks, reservoirs, washing drums, rinse chambers, washbasins or sinks, toilets, cisterns, toilet rims, siphons, water pipes and / or lines, water inlets and / or water outlets.;

[0036] This is particularly surprising since, to date, such uses of dispersins for reducing odors and / or removing bioaccumulations and / or improving hygiene have not been described.

[0037] Dispersin belongs to the group of hexosaminidase or β-N-acetylglucosaminidase (EC 3.2.1.52). These are polypeptides that catalyze the hydrolysis of N-acetyl-D-hexosamine or N-acetylglucosamine polymers. Dispersin B is a β-hexosaminidase that specifically hydrolyzes the β-1,6-glycosidic bond of acetylglucosamine polymers found in, for example, biofilms, EPS (extracellular polymeric structures), cell debris, bioaggregates, and other so-called biofoulings (i.e., stains of biological origin).

[0038] According to the present invention, a dispersin having β-1,6-N-acetylglucosaminidase activity is used and comprises an amino acid sequence that is at least 70% (and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99%) identical over its entire length to the amino acid sequence given in SEQ ID NO: 1 and is based on the amino acid sequence according to SEQ ID NO: 2. NO:1 number, in the same position as position 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71, 72, 74, 77, 79, 80, 81, 82, 90, 99, 100, 103, 104, 105, 106, 107, 110, 111 、113、114、116、117、118、119、120、122、123、124、125、126、127、128、131、135、138、139、140、142、145、147、148、149、150、151、152、163、164、167、168、170、171、173、174、175、177、178、179、181、 185, 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221, 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251, 252, 253, 254, 256, 260, 261, 262, 263, 264, 265, 2 314, 315, 319, 321 and 323 have at least one amino acid substitution and / or deletion at at least one position corresponding to 267, 268, 270, 271, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321 and 323.

[0039] According to the present invention, a dispersin having β-1,6-N-acetylglucosaminidase activity is used and comprises an amino acid sequence that is at least 70% (and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99%) identical over its entire length to the amino acid sequence given in SEQ ID NO: 1 and is based on the amino acid sequence according to SEQ ID NO: 2. 104, 105, 106, 107, 110, 111, 113, 114, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 127, 128, 131, 135, 138, 139, 140, 142, 145, 147, 148, 149, 150, 151, 152, 163, 164, 167, 168, 170, 171, 173, 174, 175, 177, 178, 179, 181, 185, 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 22 1, 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251, 252, 253, 254, 256, 260, 261, 262, 263, 264, 265, 267, 268, 270, 271, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321 and 323 have at least one amino acid substitution and / or deletion selected from the group consisting of: D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L,E19D、E19N、E19P、K22A、K22M、K22V、S23C、S23E、S23I、S23L、S23R、S23T、S23V、V25R、D26M、Y30*、Y30D、Y30L、Y30M、Y30N、Y30R、Y30T、Y30V、G32L、G32M、G32R、N43*、N43H、N43L、E44*、N45D、N45L、N45V、A49W、A49Y、Y52*、Y52M、G54L、G54M、G54N、S56T、S56W、S57W、E58N、N59A、N59C、N59D、N59E、N59F、N59M、N59R、N59V、N59W、T60V、N62C、N62D、N62H、N62Q、N62W、T63C、T63D、T63L、T63N、T63R、T63V、K67A、K67L、N68L、N68Q、L71H、L71N、L71R、L71V、L71W、S72*、S72C、S72D、S72E、S72F、S72G、S72I、S72M、S72N、S72R、S72T、S72Y、I74L、S77A、D79V、K80*、K80E、K80H、K80L、K80N、K80Q、K80V、K80W、D81A、D81G、D81L、D81R、D81S、D81T、D81V、D81W、I82V、L90F、E99Q、E99R、L100S、K103A、K103R、K104N、K104W、D105N、V106A、V106D、V106E、V106H、V106K、V106L、V106M、V106N、V106Q、V106W、V106Y、K107A、K107C、K107L、K107M、K107T、K107V、K107W、N110M、N110R、N110V、D111A、D111E、D111M、D111N、D111Q、D111R、D111V、D111W、V113T、T114C、T114S、Y116D、Y116N、Y116R、S117*、S117D、S117H、S117N、S117P、E118*、E118A、E118D、E118G、E118L、E119G、E119W、T120I、T120L、T120M、T120V、T120W、D122*、D122H、D122R、Y123W、Y124C、Y124I、Y124K、Y124L、Y124M、Y124Q、Y124R、Y124T、Y124V、Y124W、D125C、D125G、D125K、D125Q、D125R、N126V、R127D、R127H、R127K、R127L、R127M、R127Q、R127W、V128C、V128L、V128T、D131V、Q135*、Q135A、Q135D、Q135E、Q135K、Q135M、Q135Y、D138K、D138L、D138M、D138Q、D138R、D138S、D138V、D138W、E139W、D142R、D142W、Y145*、Y145H、Y145L、Y145N、Y145V、P147A、P147C、P147D、P147F、P147G、P147L、P147M、P147R、P147S、P147T、P147V、K148A、K148D、K148L、K148V、F149L、F149M、F149N、E150D、E150H、E150K、E150L、E150M、E150N、E150R、E150V、E150W、E150Y、G151A、G151C、G151D、G151L、G151N、G151P、G151S、G151W、K152D、K152L、K152R、G164D、G164E、G164H、G164S、G164V、V167D、V167E、V167L、V167P、V167Q、V167R、V167W、H168N、L170A、L170D、L170E、L170F、L170H、L170K、L170M、L170N、L170P、L170Q、L170R、L170S、L170V、L170W、L170Y、D171A、D171C、D171E、D171K、D171L、D171M、D171Q、D171R、D171V、D171W、D171Y、I173C、D174H、D174M、D174N、D174V、D174W、F175Y、N177M、Q178*、Q178A、Q178K、Q178R、Q178W、I179T、S181C、S181D、S181F、S181G、S181N、S181P、S181Q、S181T、S181V、S181W、E185M、E185R、E185V、E185W、S186D、S186E、S186H、S186I、S186K、S186L、S186M、S186N、S186Q、S186R、S186V、S186W、K187C、K187D、K187G、K187R、K187S、K187V、K187W、Y188P、E189L、E189V、E189W、S199C、S199L、S199M、S199Y、E200D、E200F、E200K、E200L、E200M、E200N、E200R、E200W、A203C、A203D、A203E、A203G、A203L、A203M、A203P、A203R、A203S、A203T、A203V、A203W、N204L、N204M、N204V、N204W、N204Y、L205I、D207A、D207C、D207E、D207G、D207N、D207Q、D207S、D207V、D207W、S208A、S208C、S208D、S208G、S208L、S208Q、S208T、S208V、S208W、S210T、Q215R、Q215M、Q215L、Q215*、S217V、T218A、T218L、T218Q、T218R、T218V、G222D、E224A、E224P、N227A、N227Q、N227R、N227S、N227T、N227K、D230*、D230R、D230T、D230W、E232D、E232V、N233H、N233Q、N233R、N233W、W234R、G235W、G235A、G235E、G235F、G235H、G235I、G235L、G235M、G235N、G235P、G235S、G235V、S237C、S237G、S237M、S237N、S237W、S237Y、Y244C、Y244E、Y244M、L249H、L249K、L249Q、L249R、L249W、L249Y、S251L、S251N、S251R、S251W、N252P、N252C、G253D、G253W、F254I、F254L、F254M、F254Y、Q256E、Q256R、N260*、N260A、N260C、N260E、N260I、N260K、N260L、N260M、N260Q、N260R、N260V、N260W、N260Y、E261*、E261A、E261D、E261R、E261W、Q262*、Q262F、Q262H、Q262W、Q262Y、M263K、M263L、M263Q、D264*、D264C、D264E、Y265F、N267S、N267T、W268C、W268E、W268M、W268R、Y270F、A271D、A271G、H272D、H272I、Y281P、Y282E、Y282N、Y281R、H272W、N273W、K274R, K274A, K274H, F276A, F276C, F276K, F276N, F276G, F276L, F276M, F276P, F276S, F276V , F276W, I278A, I278K, I278N, I278Q, I278V, S279C, S279D, S279E, S279G, S279N, D280C, D280E , Y281*, Y281A, Y281C, Y281H, Y281K, Y281N, Y281P, Y282E, Y282N, H283I, A284I, A284L, A284N , A284P, A284T, A284V, T287N, S288D, S288K, S288N, V290I, K291L, K291R, K291V, T296C, E300A , E300D, H301C, H301N, H301R, T303A, T303C, T303G, T303K, T303Q, T303R, T303W, D304C, D304 M, L305M, L305N, S306C, K308A, K308D, K308G, K308I, K308L, K308Q, K308S, K308T, K308V, K308 Y, K309A, K309C, K309D, K309H, K309L, K309M, K309N, K309Q, K309S, K309T, K309I, K312A, K312L, K312M, K312N, K312Q, K312S, K312W, E314I, E314L, E314V, L315I, L315V, R319A, N323R, and combinations thereof.

[0040] In a further preferred embodiment, a dispersin having β-1,6-N-acetylglucosaminidase activity is used and which comprises an amino acid sequence that is at least 70% (and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99%) identical over its entire length to the amino acid sequence given in SEQ ID NO: 1 and is based on the method according to SEQ ID NO: 2. 181,185,186,187,188,189,199,203,204,205,207,210,215,218,227, 314, 315, 319 and 321 have at least one amino acid substitution and / or deletion, and the at least one amino acid substitution and / or deletion is Generations and / or deletions selected from the group consisting of: D2V, Q3F, Q3I, S12A, H15Y, T17W, T17E, V18L, K22M, S23I, S23C, S23T, S23L, S23V, S23E, V25R, Y30L, A49W, A49Y, S56T, N59D, N59C, N59E, N59R, N59F, N59W, N59V, N62C, N62D, T63C, N68Q, S72D, S72E, I74L, S77A, I82V, L90F , E99Q, L100S, T114S, T114C, Y123W, Y124I, Y124M, Y124R, Y124V, Y124Q, Y124T, Y124K, D125R, D125C, D125G, D125K, D125Q, Q135M, D138R, D138K, D138Q, L170D, L170K, L170S, L170H, D171E, D171K, D171Y, D171M, D171Q, D171L, I173C,D174W, D174H, D174M, D174N, F175Y, Q178K, I179T, S181T, S181F, S181Q, S181G, S181N, S181C, E185R, E185M, E 185V, S186K, S186M, S186R, S186H, K187G, Y188P, E189V, S199C, S199L, A203G, A203E, A203V, N204L, N204Y, N2 04V, L205I, D207N, D207S, D207C, D207G, S210T, Q215R, T218Q, N227T, N227K, E232D, G235W, S237W, Y244M, Y24 4C, N252P, N252C, Q256E, N260Q, Q262H, M263Q, D264E, Y265F, N267T, N267S, Y270F, H272M, H272P, H272I, H272V , N273W, K274H, F276A, F276N, F276K, F276C, I278V, S279N, S279D, S279G, D280E, D280C, Y281P, Y282N, H283I, A284T, A284L, A284I, S288K, V290I, K291R, T296C, T303Q, D304M, D304C, L305M, L305N, S306C, K308D, K308A, K 308V, K308Q, K308S, K308Y, K308G, K308L, K308T, K308I, K309C, K309L, K309D, K309Q, K309N, K309T, K309A, K309S, K309M, K309H, K312A, K312Q, K312S, K312W, K312L, K312N, E314L, E314V, E314I, L315I, R319A, N323R, and combinations thereof.

[0041] In a further preferred embodiment, a dispersin having β-1,6-N-acetylglucosaminidase activity is used and which comprises an amino acid sequence that is at least 70% (and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99%) identical over its entire length to the amino acid sequence given in SEQ ID NO: 1 and is based on the method according to SEQ ID NO: 2. NO: 1, having at least one amino acid substitution and / or deletion at at least one position corresponding to positions 3, 15, 17, 49, 59, 140, 163, 186, 207, 215, 218, 225, 227, 232, 235, 237, 252, 260, 267, 272, 279, 281, 288, 308, 309 and 312, wherein the at least one amino acid substitution and / or deletion is selected from the group consisting of: Q3F , Q3I, H15Y, T17W, A49W, N59E, V140I, S163P, S186R, D207N, Q215K, T218Q, S225G, N227T, E232D, G235W, S237W, N252P, N260Q, N267T, H272V, H272P, S279D, Y281P, S288P, K308Q, K309E, K312Q, and combinations thereof.

[0042] In a particularly preferred embodiment, a dispersin having β-1,6-N-acetylglucosaminidase activity is used and which comprises an amino acid sequence that is at least 70% (and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99%) identical over its entire length to the amino acid sequence given in SEQ ID NO: 1 and is based on the method according to SEQ ID NO: 2. NO:1, having at least one amino acid substitution and / or deletion at at least one position corresponding to position 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281, 308, 309 and 312, wherein the at least one amino acid substitution and / or deletion is selected from the following: Q3I, H15Y, A49W, N59E, S163P, S186R, S225G, N227T, E232D, G235W, N252P, N260Q, H272V, S279D, Y281P, K308Q, K309E, K312Q and combinations thereof.

[0043] In a preferred embodiment, a dispersin having β-1,6-N-acetylglucosaminidase activity is used and comprises an amino acid sequence that is at least 70% (and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99%) identical over its entire length to the amino acid sequence given in SEQ ID NO: 1 and having at least one (and increasingly preferably two, three, four, five, six, seven, eight or nine) amino acid substitution(s), wherein the dispersin is based on the amino acid sequence given in SEQ ID NO: 1. The numbering of NO: 1 has an amino acid substitution combination selected from the following: Q3I-A49W, Q3I-N59E, Q3I-S163P, Q3I-S186R, Q3I-Q215K, Q3I-S225G, Q3I-N227T, Q3I-N252P, Q3I-N267T, Q3I-F276A, Q3I-Y281P, Q3I-K308Q, Q3I-K308E, Q3I-K309E, Q3I-K312Q, Q3I-K312E , Q3I-H15Y, Q3I-E232D, Q3I-G235W, Q3I-N260Q, Q3I-H272V, Q3I-S279D, Q3I-H15Y-A49W, Q3I-H15Y-N59E, Q 3I-H15Y-S163P, Q3I-H15Y-S186R, Q3I-H15Y-S225G, Q3I-H15Y-N227T, Q3I-H15Y-E232D, Q3I-H15Y-G235W, Q 3I-H15Y-N252P, Q3I-H15Y-N260Q, Q3I-H15Y-H272V, Q3I-H15Y-S279D, Q3I-H15Y-Y281P, Q3I-H15Y-K308Q, Q3I-H15Y-K309E, Q3I-H15Y-K312Q, Q3I-A49W-N59E, Q3I-A49W-S163P, Q3I-A49W-S186R, Q3I-A49W-S225G, Q 3I-A49W-N227T, Q3I-A49W-E232D, Q3I-A49W-G235W, Q3I-A49W-N252P, Q3I-A49W-N260Q, Q3I-A49W-H272V, Q3I-A49W-S279D, Q3I-A49W-Y281P, Q3I-A49W-K308Q, Q3I-A49W-K309E, Q3I-A49W-K312Q, Q3I-N59E-S163P,<h2 style=";text-align:left;direction:ltr">Q3I-N59E-S186R、Q3I-N59E-S225G、Q3I-N59E-N227T、Q3I-N59E-E232D、Q3I-N59E-G235W、Q3I-N59E-N252P、Q3I-N59E-N260Q、Q3I-N59E-H272V、Q3I- N59E-S279D、Q3I-N59E-Y281P、Q3I-N59E-K308Q、Q3I-N59E-K309E、Q3I-N59E-K312Q、Q3I-S163P-S186R、Q3I-S163P-S225G、Q3I-S163P-N227T、Q3I-S 163P-E232D、Q3I-S163P-G235W、Q3I-S163P-N252P、Q3I-S163P-N260Q、Q3I -S163P-H272V、Q3I-S163P-S279D、Q3I-S163P-Y281P、Q3I-S163P-K308Q、Q 3I-S163P-K309E、Q3I-S163P-K312Q、Q3I-S186R-S225G、Q3I-S186R-N227T、Q3I-S186R-E232D、Q3I-S186R-G235W、Q3I-S186R-N252P、Q3I-S186R-N260 Q、Q3I-S186R-H272V、Q3I-S186R-S279D、Q3I-S186R-Y281P、Q3I-S186R-K3 08Q、Q3I-S186R-K309E、Q3I-S186R-K312Q、Q3I-S225G-N227T、Q3I-S225G- E232D、Q3I-S225G-G235W、Q3I-S225G-N252P、Q3I-S225G-N260Q、Q3I-S225G-H272V、Q3I-S225G-S279D、Q3I-S225G-Y281P、Q3I-S225G-K308Q、Q3I-S22 5G-K309E、Q3I-S225G-K312Q、Q3I-N227T-E232D、Q3I-N227T-G235W、Q3I-N227T-N252P、Q3I-N227T-N260Q、Q3I-N227T-H272V、Q3I-N227T-S279D、Q3I -N227T-Y281P、Q3I-N227T-K308Q、Q3I-N227T-K309E、Q3I-N227T-K312Q、Q3I-E232D-G235W、Q3I-E232D-N252P、Q3I-E232D-N260Q、Q3I-E232D-H272V、<h2 style=";text-align:left;direction:ltr">Q3I-E232D-S279D、Q3I-E232D-Y281P、Q3I-E232D-K308Q、Q3I-E232D-K309E、Q3I-E232D-K312Q、Q3I-G235W-N252P、Q3I-G235W-N260Q、Q3I-G235 W-H272V、Q3I-G235W-S279D、Q3I-G235W-Y281P、Q3I-G235W-K308Q、Q3I-G235W-K309E、Q3I-G235W-K312Q、Q3I-N252P-N260Q、Q3I-N252P-H272V、 Q3I-N252P-S279D、Q3I-N252P-Y281P、Q3I-N252P-K308Q、Q3I-N252P-K309E、Q3I-N252P-K312Q、Q3I-N260Q-H272V、Q3I-N260Q-S279D、Q3I-N260 Q-Y281P、Q3I-N260Q-K308Q、Q3I-N260Q-K309E、Q3I-N260Q-K312Q、Q3I-H272V-S279D、Q3I-H272V-Y281P、Q3I-H272V-K308Q、Q3I-H272V-K309E、Q 3I-H272V-K312Q、Q3I-S279D-Y281P、Q3I-S279D-K308Q、Q3I-S279D-K309E、Q3I-S279D-K312Q、Q3I-Y281P-K308Q、Q3I-Y281P-K309E、Q3I-Y281P -K312Q、Q3I-K308Q-K309E、Q3I-K308Q-K312Q、Q3I-K309E-K312Q、Q3I-Q225G-N227T-N252P-N260Q-S186R-K308Q-K309E-K312Q、Q3I-S163P-Q225 G-N227T-N252P-N260Q-K308Q-K309E-K312Q、Q3F-A49W、Q3F-N59E、Q3F- S163P、Q3F-S186R、Q3F-Q215K、Q3F-S225G、Q3F-N227T、Q3F-N252P、Q3F- N267T、Q3F-F276A、Q3F-Y281P、Q3F-K308Q、Q3F-K308E、Q3F-K309E、Q3F-K312E、Q3F-K312Q、Q3F-A49W-Q215K-F276A-K308E-K309E-K312E-N227T、Q3F-A49W-Q215K-F276A-K308E-K309E-K312E-N267T、Q3F-A49W-Q215K-F276A-K308E-K309E-N227T-N267T、Q3F-A49W-Q215K-F276A-K308E-K312E-N227T-N267T、Q3F-A49W-Q215K-F276A-K309E-K312E-N227T-N267T、Q3F-A49W-Q215K-K308E-K309E-K312E-N227T-N267T、Q3F-A49W-F276A-K308E-K309E-K312E-N227T-N267T、Q3F-Q215K-F276A-K308E-K309E-K312E-N227T-N267T、Q3F-Q215K-N227T-N252P-N267T-F276A-K308E-K309E-K312E、Q3F-S163P-Q215K-N227T-N252P-N267T-K308E-K309E-K312E、Q3F-A49W-Q215K-F276A-K308E-K309E-K312E-N227T-N267T、H15Y-A49W、H15Y-N59E、H15Y-S163P、H15Y-S186R、H15Y-S225G、H15Y-N227T、H15Y-E232D、H15Y-235W、H15Y-N252P、H15Y-N260Q、H15Y-H272V、H15Y-S279D、H15Y-Y281P、H15Y-K308Q、H15Y-K309E、H15Y-K312Q、H15Y-A49W-N59E、H15Y-A49W-S163P、H15Y-A49W-S186R、H15Y-A49W-S225G、H15Y-A49W-N227T、H15Y-A49W-E232D、H15Y-A49W-G235W、H15Y-A49W-N252P、H15Y-A49W-N260Q、H15Y-A49W-H272V、H15Y-A49W-S279D、H15Y-A49W-Y281P、H15Y-A49W-K308Q、H15Y-A49W-K309E、H15Y-A49W-K312Q、H15Y-N59E-S163P、H15Y-N59E-S186R、H15Y-N59E-S225G、H15Y-N59E-N227T、H15Y-N59E-E232D、H15Y-N59E-G235W、H15Y-N59E-N252P、H15Y-N59E-N260Q、H15Y-N59E-H272V、H15Y-N59E-S279D、H15Y-N59E-Y281P、H15Y-N59E-K308Q、H15Y-N59E-K309E、H15Y-N59E-K312Q、H15Y-S163P-S186R、H15Y-S163P-S225G、H15Y-S163P-N227T、H15Y-S163P-E232D、H15Y-S163P-G235W、H15Y-S163P-N252P、H15Y-S163P-N260Q、H15Y-S163P-H272V、H15Y-S163P-S279D、H15Y-S163P-Y281P、H15Y-S163P-K308Q、H15Y-S163P-K309E、H15Y-S163P-K312Q、H15Y-S186R-S225G、H15Y-S186R-N227T、H15Y-S186R-E232D、H15Y-S186R-G235W、H15Y-S186R-N252P、H15Y-S186R-N260Q、H15Y-S186R-H272V、H15Y-S186R-S279D、H15Y-S186R-Y281P、H15Y-S186R-K308Q、H15Y-S186R-K309E、H15Y-S186R-K312Q、H15Y-S225G-N227T、H15Y-S225G-E232D、H15Y-S225G-G235W、H15Y-S225G-N252P、H15Y-S225G-N260Q、H15Y-S225G-H272V、H15Y-S225G-S279D、H15Y-S225G-Y281P、H15Y-S225G-K308Q、H15Y-S225G-K309E、H15Y-S225G-K312Q、H15Y-N227T-E232D、H15Y-N227T-G235W、H15Y-N227T-N252P、H15Y-N227T-N260Q、H15Y-N227T-H272V、H15Y-N227T-S279D、H15Y-N227T-Y281P、H15Y-N227T-K308Q、H15Y-N227T-K309E、H15Y-N227T-K312Q、H15Y-E232D-G235W、H15Y-E232D-N252P、H15Y-E232D-N260Q、H15Y-E232D-H272V、H15Y-E232D-S279D、H15Y-E232D-Y281P、H15Y-E232D-K308Q、H15Y-E232D-K309E、H15Y-E232D-K312Q、H15Y-G235W-N252P、H15Y-G235W-N260Q、H15Y-G235W-H272V、H15Y-G235W-S279D、H15Y-G235W-Y281P、H15Y-G235W-K308Q、H15Y-G235W-K309E、H15Y-G235W-K312Q、H15Y-N252P-N260Q、H15Y-N252P-H272V、H15Y-N252P-S279D、H15Y-N252P-Y281P、H15Y-N252P-K308Q、H15Y-N252P-K309E、H15Y-N252P-K312Q、H15Y-N260Q-H272V、H15Y-N260Q-S279D、H15Y-N260Q-Y281P、H15Y-N260Q-K308Q、H15Y-N260Q-K309E、H15Y-N260Q-K312Q、H15Y-H272V-S279D、H15Y-H272V-Y281P、H15Y-H272V-K308Q、H15Y-H272V-K309E、H15Y-H272V-K312Q、H15Y-S279D-Y281P、H15Y-S279D-K308Q、H15Y-S279D-K309E、H15Y-S279D-K312Q、H15Y-Y281P-K308Q、H15Y-Y281P-K309E、H15Y-Y281P-K312Q、H15Y-K308Q-K309E、H15Y-K308Q-K312Q、H15Y-K309E-K312Q、A49W-N59E、A49W-S163P、A49W-S186R、A49W-Q215K、A49W-S225G、A49W-N227T、A49W-N252P、A49W-N267T、A49W-F276A、A49W-Y281P、A49W-K308Q、A49W-K308E、A49W-K309E、A49W-K312Q、A49W-K312E、A49W-E232D、A49W-G235W、A49W-N260Q、A49W-H272V、A49W-S279D、A49W-N59E-S163P、A49W-N59E-S186R、A49W-N59E-S225G、A49W-N59E-N227T、A49W-N59E-E232D、A49W-N59E-G235W、A49W-N59E-N252P、<h2 style=";text-align:left;direction:ltr">A49W-N59E-N260Q、A49W-N59E-H272V、A49W-N59E-S279D、A49W-N59E-Y281 P、A49W-N59E-K308Q、A49W-N59E-K309E、A49W-N59E-K312Q、A49W-S163P-S 186R、A49W-S163P-S225G、A49W-S163P-N227T、A49W-S163P-E232D、A49W-S 163P-G235W、A49W-S163P-N252P、A49W-S163P-N260Q、A49W-S163P-H272V、A 49W-S163P-S279D、A49W-S163P-Y281P、A49W-S163P-K308Q、A49W-S163P-K 309E、A49W-S163P-K312Q、A49W-S186R-S225G、A49W-S186R-N227T、A49W-S 186R-E232D、A49W-S186R-G235W、A49W-S186R-N252P、A49W-S186R-N260Q、 A49W-S186R-H272V、A49W-S186R-S279D、A49W-S186R-Y281P、A49W-S186R-K 308Q、A49W-S186R-K309E、A49W-S186R-K312Q、A49W-S225G-N227T、A49W-S 225G-E232D、A49W-S225G-G235W、A49W-S225G-N252P、A49W-S225G-N260Q、 A49W-S225G-H272V、A49W-S225G-S279D、A49W-S225G-Y281P、A49W-S225G-K308Q、A49W-S225G-K309E、A49W-S225G-K312Q、A49W-N227T-E232D、A49W-N 227T-G235W、A49W-N227T-N252P、A49W-N227T-N260Q、A49W-N227T-H272V、A49W-N227T-S279D、A49W-N227T-Y281P、A49W-N227T-K308Q、A49W-N227T- K309E、A49W-N227T-K312Q、A49W-E232D-G235W、A49W-E232D-N252P、A49W-E232D-N260Q、A49W-E232D-H272V、A49W-E232D-S279D、A49W-E232D-Y281P、<h2 style=";text-align:left;direction:ltr">A49W-E232D-K308Q、A49W-E232D-K309E、A49W-E232D-K312Q、A49W-G235 W-N252P、A49W-G235W-N260Q、A49W-G235W-H272V、A49W-G235W-S279D、A4 9W-G235W-Y281P、A49W-G235W-K308Q、A49W-G235W-K309E、A49W-G235W-K312Q、A49W-N252P-N260Q、A49W-N252P-H272V、A49W-N252P-S279D、A49W -N252P-Y281P、A49W-N252P-K308Q、A49W-N252P-K309E、A49W-N252P-K312Q、A49W-N260Q-H272V、A49W-N260Q-S279D、A49W-N260Q-Y281P、A49W-N 260Q-K308Q、A49W-N260Q-K309E、A49W-N260Q-K312Q、A49W-H272V-S279D、A49W-H272V-Y281P、A49W-H272V-K308Q、A49W-H272V-K309E、A49W-H27 2V-K312Q、A49W-S279D-Y281P、A49W-S279D-K308Q、A49W-S279D-K309E、A49W-S279D-K312Q、A49W-Y281P-K308Q、A49W-Y281P-K309E、A49W-Y281P -K312Q、A49W-K308Q-K309E、A49W-K308Q-K312Q、A49W-K309E-K312Q、A4 9W-N227T-F276A-K308E、A49W-S186R-N227T-F276A、A49W-S186R-N227T- K308E、A49W-N227T-A269C-Y282C-K308E、A49W-S186R-N227T-F276A-K308E、A49W-Y124R-N227T-F276A-K308E、A49W-Y124R-S186R-N227T-F276A 、A49W-Y124R-S186R-N227T-K308E、A49W-A269C-F276A-Y282C-K308E、A49W-N227T-A269C-F276A-Y282C、A49W-F276A-K308E、A49W-N227T-F276A、<h2 style=";text-align:left;direction:ltr">A49W-Y124R-S186R-N227T-F276A-K308E、A49W-N227T-A269C-F276A-Y282C-K308E、A49W-S186R-N227T-A269C-F276A-Y282C、A49W-S186R-N227T-A2 69C-Y282C-K308E、A49W-S186R-N227T-A269C-F276A-Y282C-K308E、A49W-Y124R-N227T-A269C-F276A-Y282C-K308E、A49W-Y124R-S186R-N227T-A26 9C-F276A-Y282C、A49W-Y124R-S186R-N227T-A269C-Y282C-K308E、A49W-Y124R-S186R-N227T-A269C-F276A-Y282C-K308E、A49W-S163P-Q215K-N227 T-N252P-N267T-F276A-K309E-K312E、A49W-Q215K-N227T-N252P-N267T-F 276A-K308E-K309E-K312E、A49W-S163P-Q225G-N227T-N252P-N260Q-S186 R-K309E-K312Q、A49W-Q225G-N227T-N252P-N260Q-S186R-K308Q-K309E-K 312Q、N59E-S163P、N59E-S186R、N59E-Q215K、N59E-S225G、N59E-N227T、N5 9E-N252P、N59E-N267T、N59E-F276A、N59E-Y281P、N59E-K308Q、N59E-K308 E、N59E-K309E、N59E-K312Q、N59E-K312E、N59E-E232D、N59E-G235W、N59E- N260Q、N59E-H272V、N59E-S279D、N59E-S163P-S186R、N59E-S163P-S225G、N59E-S163P-N227T、N59E-S163P-E232D、N59E-S163P-G235W、N59E-S163P- N252P、N59E-S163P-N260Q、N59E-S163P-H272V、N59E-S163P-S279D、N59E-S163P-Y281P、N59E-S163P-K308Q、N59E-S163P-K309E、N59E-S163P-K312Q、<h2 style=";text-align:left;direction:ltr">N59E-S186R-S225G、N59E-S186R-N227T、N59E-S186R-E232D、N59E-S186R -G235W、N59E-S186R-N252P、N59E-S186R-N260Q、N59E-S186R-H272V、N59E -S186R-S279D、N59E-S186R-Y281P、N59E-S186R-K308Q、N59E-S186R-K30 9E、N59E-S186R-K312Q、N59E-S225G-N227T、N59E-S225G-E232D、N59E-S22 5G-G235W、N59E-S225G-N252P、N59E-S225G-N260Q、N59E-S225G-H272V、N 59E-S225G-S279D、N59E-S225G-Y281P、N59E-S225G-K308Q、N59E-S225G-K 309E、N59E-S225G-K312Q、N59E-N227T-E232D、N59E-N227T-G235W、N59E-N227T-N252P、N59E-N227T-N260Q、N59E-N227T-H272V、N59E-N227T-S279D、 N59E-N227T-Y281P、N59E-N227T-K308Q、N59E-N227T-K309E、N59E-N227T-K312Q、N59E-E232D-G235W、N59E-E232D-N252P、N59E-E232D-N260Q、N59E -E232D-H272V、N59E-E232D-S279D、N59E-E232D-Y281P、N59E-E232D-K308Q、N59E-E232D-K309E、N59E-E232D-K312Q、N59E-G235W-N252P、N59E-G23 5W-N260Q、N59E-G235W-H272V、N59E-G235W-S279D、N59E-G235W-Y281P、N 59E-G235W-K308Q、N59E-G235W-K309E、N59E-G235W-K312Q、N59E-N252P-N 260Q、N59E-N252P-H272V、N59E-N252P-S279D、N59E-N252P-Y281P、N59E-N252P-K308Q、N59E-N252P-K309E、N59E-N252P-K312Q、N59E-N260Q-H272V、<h2 style=";text-align:left;direction:ltr">N59E-N260Q-S279D、N59E-N260Q-Y281P、N59E-N260Q-K308Q、N59E-N260Q-K309E、N59E-N260Q-K312Q、N59E-H272V-S279D、N59E-H272V-Y281P、N59E- H272V-K308Q、N59E-H272V-K309E、N59E-H272V-K312Q、N59E-S279D-Y281P、N59E-S279D-K308Q、N59E-S279D-K309E、N59E-S279D-K312Q、N59E-Y281P- K308Q、N59E-Y281P-K309E、N59E-Y281P-K312Q、N59E-K308Q-K309E、N59E-K308Q-K312Q、N59E-K309E-K312Q、N59E-N227T-N252P-E232D-S225G、N59E- N227T-N252P-E232D-K308Q、N59E-N227T-N252P-E232D-K309E、N59E-N227T-N252P-S225G-K308Q、N59E-N227T-N252P-S225G-K309E、N59E-N227T-N25 2P-K308Q-K309E、N59E-N227T-E232D-S225G-K308Q、N59E-N227T-E232D-S225G-K309E、N59E-N227T-E232D-K308Q-K309E、N59E-N227T-S225G-K308Q- K309E、N59E-N252P-E232D-S225G-K308Q、N59E-N252P-E232D-S225G-K309E、N59E-N252P-E232D-K308Q-K309E、N59E-N252P-S225G-K308Q-K309E、N59 E-E232D-S225G-K308Q-K309E、N59E-N227T-S225G-K308Q-K312Q、N59E-N2 27T-S225G-K309E-K312Q、N59E-N227T-K308Q-K309E-K312Q、N59E-S225G-K 308Q-K309E-K312Q、Y124R-S186R-N227T-F276A-K308E、Y124R-S186R-N22 7T-A269C-F276A-Y282C-K308E、S163P-S186R、S163P-Q215K、S163P-S225G、S163P-N227T、S163P-N252P、S163P-N267T、S163P-F276A、S163P-Y281P、S163P-K308E、S163P-K308Q、S163P-K309E、S163P-K312Q、S163P-K312E、S163P-E232D、S163P-G235W、S163P-N260Q、S163P-H272V、S163P-S279D、S163P-S186R-S225G、S163P-S186R-N227T、S163P-S186R-E232D、S163P-S186R-G235W、S163P-S186R-N252P、S163P-S186R-N260Q、S163P-S186R-H272V、S163P-S186R-S279D、S163P-S186R-Y281P、S163P-S186R-K308Q、S163P-S186R-K309E、S163P-S186R-K312Q、S163P-S225G-N227T、S163P-S225G-E232D、S163P-S225G-G235W、S163P-S225G-N252P、S163P-S225G-N260Q、S163P-S225G-H272V、S163P-S225G-S279D、S163P-S225G-Y281P、S163P-S225G-K308Q、S163P-S225G-K309E、S163P-S225G-K312Q、S163P-N227T-E232D、S163P-N227T-G235W、S163P-N227T-N252P、S163P-N227T-N260Q、S163P-N227T-H272V、S163P-N227T-S279D、S163P-N227T-Y281P、S163P-N227T-K308Q、S163P-N227T-K309E、S163P-N227T-K312Q、S163P-E232D-G235W、S163P-E232D-N252P、S163P-E232D-N260Q、S163P-E232D-H272V、S163P-E232D-S279D、S163P-E232D-Y281P、S163P-E232D-K308Q、S163P-E232D-K309E、S163P-E232D-K312Q、S163P-G235W-N252P、S163P-G235W-N260Q、S163P-G235W-H272V、S163P-G235W-S279D、S163P-G235W-Y281P、S163P-G235W-K308Q、S163P-G235W-K309E、S163P-G235W-K312Q、S163P-N252P-N260Q、S163P-N252P-H272V、S163P-N252P-S279D、S163P-N252P-Y281P、S163P-N252P-K308Q、S163P-N252P-K309E、S163P-N252P-K312Q、S163P-N260Q-H272V、S163P-N260Q-S279D、S163P-N260Q-Y281P、S163P-N260Q-K308Q、S163P-N260Q-K309E、S163P-N260Q-K312Q、S163P-H272V-S279D、S163P-H272V-Y281P、S163P-H272V-K308Q、S163P-H272V-K309E、S163P-H272V-K312Q、S163P-S279D-Y281P、S163P-S279D-K308Q、S163P-S279D-K309E、S163P-S279D-K312Q、S163P-Y281P-K308Q、S163P-Y281P-K309E、S163P-Y281P-K312Q、S163P-K308Q-K309E、S163P-K308Q-K312Q、S163P-K309E-K312Q、S163P-F276A-K308E、S163P-F276A-K312E、S163P-K308E-K309E、S163P-K308E-K312E、S163P-K309E-K312E、S163P-N227T-F276A、S163P-N227T-N267T、S163P-N252P-F276A、S163P-N252P-K308E、S163P-N252P-K312E、S163P-N252P-N267T、S163P-N267T-K308E、S163P-N267T-K309E、S163P-N267T-K312E、S163P-Q215K-F276A、S163P-Q215K-K308E、S163P-Q215K-K309E、S163P-Q215K-K312E、S163P-Q215K-N227T、S163P Q215K N252P、S163P-Q215K-N252P、S163P-N227T-F276A-K308E、S163P-N227T-S225G-K308Q、S163P-Q215K-N227T-N252P-N267T、S163P-Q215K-N227T-N252P-F276A、S163P-Q215K-N227T-N252P-K308E、S163P-Q215K-N227T-N252P-K309E、S163P-Q215K-N227T-N267T-F276A、S163P-Q215K-N227T-N267T-K308E、S163P-Q215K-N227T-N267T-K309E、S163P-Q215K-N227T-F276A-K308E、S163P-Q215K-N227T-F276A-K309E、S163P-Q215K-N227T-K308E-K309E、S163P-Q215K-N252P-N267T-F276A、S163P-Q215K-N252P-N267T-K308E、S163P-Q215K-N252P-N267T-K309E、S163P-Q215K-N252P-F276A-K308E、S163P-Q215K-N252P-F276A-K309E、S163P-Q215K-N252P-K308E-K309E、S163P-Q215K-N267T-F276A-K308E、S163P-Q215K-N267T-F276A-K309E、S163P-Q215K-N267T-K308E-K309E、S163P-Q215K-F276A-K308E-K309E、S163P-N227T-N252P-N267T-F276A、S163P-N227T-N252P-N267T-K308E、S163P-N227T-N252P-N267T-K309E、S163P-N227T-N252P-F276A-K308E、S163P-N227T-N252P-F276A-K309E、S163P-N227T-N252P-K308E-K309E、S163P-N227T-N267T-F276A-K308E、S163P-N227T-N267T-F276A-K309E、S163P-N227T-N267T-K308E-K309E、S163P-N227T-F276A-K308E-K309E、S163P-N252P-N267T-F276A-K308E、S163P-N252P-N267T-F276A-K309E、<h2 style=";text-align:left;direction:ltr">S163P-N252P-N267T-K308E-K309E、S163P-N252P-F276A-K308E-K309E、S1 63P-N267T-F276A-K308E-K309E、S163P-N59E-N227T-N252P-E232D、S163P- N59E-N227T-N252P-S225G、S163P-N59E-N227T-N252P-K308Q、S163P-N59E-N227T-N252P-K309E、S163P-N59E-N227T-E232D-S225G、S163P-N59E-N227 T-E232D-K308Q、S163P-N59E-N227T-E232D-K309E、S163P-N59E-N227T-S2 25G-K308Q、S163P-N59E-N227T-S225G-K309E、S163P-N59E-N227T-K308Q-K 309E、S163P-N59E-N252P-E232D-S225G、S163P-N59E-N252P-E232D-K308Q、S163P-N59E-N252P-E232D-K309E、S163P-N59E-N252P-S225G-K308Q、S163 P-N59E-N252P-S225G-K309E、S163P-N59E-N252P-K308Q-K309E、S163P-N59E-E232D-S225G-K308Q、S163P-N59E-E232D-S225G-K309E、S163P-N59E-E2 32D-K308Q-K309E、S163P-N59E-S225G-K308Q-K309E、S163P-N227T-N252P-E232D-S225G、S163P-N227T-N252P-E232D-K308Q、S163P-N227T-N252P-E2 32D-K309E、S163P-N227T-N252P-S225G-K308Q、S163P-N227T-N252P-S225 G-K309E、S163P-N227T-N252P-K308Q-K309E、S163P-N227T-E232D-S225G-K 308Q、S163P-N227T-E232D-S225G-K309E、S163P-N227T-E232D-K308Q-K30 9E、S163P-N227T-S225G-K308Q-K309E、S163P-N252P-E232D-S225G-K308Q、S163P-N252P-E232D-S225G-K309E、S163P-N252P-E232D-K308Q-K309E、S163P-N252P-S225G-K308Q-K309E、S163P-E232D-S225G-K308Q-K309E、S163P-N227T-F276A-K308E-K312E、S163P-N227T-S225G-K308Q-K312Q、N227T-A269C-F276A-Y282C-K308E、S163P-N227T-F276A-K308E-K309E-K312E、S163P-N227T-S186R-K308Q-K309E-K312Q、S163P-Q215K-N227T-N252P-N267T-F276A、S163P-N227T-N252P-F276A-K308E-K309E-K312E、S163P-N227T-N267T-F276A-K308E-K309E-K312E、S163P-Q215K-N227T-F276A-K308E-K309E-K312E、S163P-N227T-S225G-S186R-K308Q-K309E-K312Q、S163P-S186R-N227T-S186R-K308Q-K309E-K312Q、S163P-Q215K-N227T-N252P-N267T-F276A-K308E、S163P-Q215K-N227T-N252P-N267T-F276A-K309E、S163P-Q215K-N227T-N252P-N267T-F276A-K312E、S163P-Q215K-N227T-N252P-N267T-F276A-K308E-K309E、S163P-Q215K-N227T-N252P-N267T-F276A-K308E-K312E、S163P-Q215K-N227T-N252P-N267T-F276A-K309E-K312E、S163P-Q215K-N227T-N252P-N267T-K308E-K309E-K312E、S163P-Q215K-N227T-N252P-F276A-K308E-K309E-K312E、S163P-Q215K-N227T-N267T-F276A-K308E-K309E-K312E、S163P-Q215K-N252P-N267T-F276A-K308E-K309E-K312E、S163P-N227T-N252P-N267T-F276A-K308E-K309E-K312E、S163P-G235W-N227T-N252P-S186R-N260Q-K308Q-K309E、S163P-G235W-N227T-N252P-S186R-N260Q-K308Q-K312Q、S163P-G235W-N227T-N252P-S186R-N260Q-K309E-K312Q、S163P-G235W-N227T-N252P-S186R-K308Q-K309E-K312Q、S163P-G235W-N227T-N252P-N260Q-K308Q-K309E-K312Q、S163P-G235W-N227T-S186R-N260Q-K308Q-K309E-K312Q、S163P-G235W-N252P-S186R-N260Q-K308Q-K309E-K312Q、S163P-N227T-N252P-S186R-N260Q-K308Q-K309E-K312Q、S163P-N227T-N252P-S186R-N260Q-K308E-K309E-K312E、S163P-S225G-N227T-N252P-N260Q-K308E-K309E-K312E、S163P-S225G-N227T-S186R-N260Q-K308E-K309E-K312E、S163P-Q215K-N227T-N252P-N267T-F276A-K308E-K309E-K312E、S163P-Q225G-N227T-N252P-N260Q-S186R-K308Q-K309E-K312Q、S163P-S186R-S225G-N227T-G235W-N252P-K308Q-K309E-K312Q、S163P-Q215K-N227T-N252P-N267T-F276A-K308Q-K309E-K312E、S163P-Q215K-N227T-N252P-N267T-F276A-K308E-K309E-K312Q、S186R-Q215K、S186R-S225G、S186R-N227T、S186R-N252P、S186R-N267T、S186R-F276A、S186R-Y281P、S186R-K308E、S186R-K308Q、S186R-K309E、S186R-K312Q、S186R-K312E、S186R-E232D、S186R-G235W、S186R-N260Q、S186R-H272V、S186R-S279D、S186R-S225G-N227T、S186R-S225G-E232D、S186R-S225G-G235W、S186R-S225G-N252P、S186R-S225G-N260Q、S186R-S225G-H272V、S186R-S225G-S279D、S186R-S225G-Y281P、S186R-S225G-K308Q、S186R-S225G-K309E、S186R-S225G-K312Q、S186R-N227T-E232D、S186R-N227T-G235W、S186R-N227T-N252P、S186R-N227T-N260Q、S186R-N227T-H272V、S186R-N227T-S279D、S186R-N227T-Y281P、S186R-N227T-K308Q、S186R-N227T-K309E、S186R-N227T-K312Q、S186R-E232D-G235W、S186R-E232D-N252P、S186R-E232D-N260Q、S186R-E232D-H272V、S186R-E232D-S279D、S186R-E232D-Y281P、S186R-E232D-K308Q、S186R-E232D-K309E、S186R-E232D-K312Q、S186R-G235W-N252P、S186R-G235W-N260Q、S186R-G235W-H272V、S186R-G235W-S279D、S186R-G235W-Y281P、S186R-G235W-K308Q、S186R-G235W-K309E、S186R-G235W-K312Q、S186R-N252P-N260Q、S186R-N252P-H272V、S186R-N252P-S279D、S186R-N252P-Y281P、S186R-N252P-K308Q、S186R-N252P-K309E、S186R-N252P-K312Q、S186R-N260Q-H272V、S186R-N260Q-S279D、S186R-N260Q-Y281P、S186R-N260Q-K308Q、S186R-N260Q-K309E、S186R-N260Q-K312Q、S186R-H272V-S279D、S186R-H272V-Y281P、S186R-H272V-K308Q、S186R-H272V-K309E、S186R-H272V-K312Q、S186R-S279D-Y281P、S186R-S279D-K308Q、S186R-S279D-K309E、S186R-S279D-K312Q、S186R-Y281P-K308Q、S186R-Y281P-K309E、S186R-Y281P-K312Q、S186R-K308Q-K309E、S186R-K308Q-K312Q、S186R-K309E-K312Q、S186R-F276A-K308E、S186R-N227T-F276A、S186R-N227T-K308E、S186R-N227T-E232D-K308E、S186R-A269C-F276A-Y282C-K308E、S186R-N227T-A269C-F276A-Y282C、S186R-N227T-A269C-Y282C-K308E、S186R-N227T-A269C-F276A-Y282C-K308E、S186R-N227T-N252P-S186R-K308Q-K309E-K312Q、S186R-N227T-S225G-S186R-K308Q-K309E-K312Q、S163P-N227T-N252P-S186R-K308Q-K309E-K312Q、Q215K-S225G、Q215K-N227T、Q215K-N252P、Q215K-N267T、Q215K-F276A、Q215K-Y281P、Q215K-K308E、Q215K-K308Q、Q215K-K309E、Q215K-K312Q、Q215K-K312E、Q215K-F276A-K308E、Q215K-F276A-K312E、Q215K-K308E-K312E、Q215K-K309E-K312E、Q215K-N227T-F276A、Q215K-N227T-K308E、Q215K-N227T-K309E、Q215K-N227T-K312E、Q215K-N227T-N252P、Q215K-N227T-N267T、Q215K-N252P-F276A、Q215K-N252P-K309E、Q215K-N252P-K312E、Q215K-N252P-N267T、Q215K-N267T-F276A、Q215K-N267T-K312E、Q215K-N227T-N252P-N267T-F276A、Q215K-N227T-N252P-N267T-K308E、Q215K-N227T-N252P-N267T-K309E、Q215K-N227T-N252P-F276A-K308E、Q215K-N227T-N252P-F276A-K309E、Q215K-N227T-N252P-K308E-K309E、Q215K-N227T-N267T-F276A-K308E、Q215K-N227T-N267T-F276A-K309E、Q215K-N227T-N267T-K308E-K309E、Q215K-N227T-F276A-K308E-K309E、Q215K-N252P-N267T-F276A-K308E、Q215K-N252P-N267T-F276A-K309E、Q215K-N252P-N267T-K308E-K309E、Q215K-N252P-F276A-K308E-K309E、Q215K-N267T-F276A-K308E-K309E、Q215K-N227T-F276A-K308E-K312E、Q215K-N227T-F276A-K309E-K312E、Q215K-N227T-K308E-K309E-K312E、Q215K-F276A-K308E-K309E-K312E、Q215K-N227T-F276A-K308E-K309E-K312E、Q215K-N227T-N252P-F276A-K308E-K309E-K312E、Q215K-N227T-N267T-F276A-K308E-K309E-K312E、Q215K-N227T-N252P-N267T-F276A-K308E-K309E-K312E、S225G-N227T、S225G-N252P、S225G-N267T、S225G-F276A、S225G-Y281P、S225G-K308E、S225G-K308Q、S225G-K309E、S225G-K312Q、S225G-K312E、S225G-E232D、S225G-G235W、S225G-N260Q、S225G-H272V、S225G-S279D、S225G-N227T-E232D、S225G-N227T-G235W、S225G-N227T-N252P、S225G-N227T-N260Q、S225G-N227T-H272V、S225G-N227T-S279D、S225G-N227T-Y281P、S225G-N227T-K308Q、S225G-N227T-K309E、S225G-N227T-K312Q、S225G-E232D-G235W、S225G-E232D-N252P、S225G-E232D-N260Q、S225G-E232D-H272V、S225G-E232D-S279D、S225G-E232D-Y281P、S225G-E232D-K308Q、S225G-E232D-K309E、S225G-E232D-K312Q、S225G-G235W-N252P、S225G-G235W-N260Q、S225G-G235W-H272V、S225G-G235W-S279D、S225G-G235W-Y281P、S225G-G235W-K308Q、S225G-G235W-K309E、S225G-G235W-K312Q、S225G-N252P-N260Q、S225G-N252P-H272V、S225G-N252P-S279D、S225G-N252P-Y281P、S225G-N252P-K308Q、S225G-N252P-K309E、S225G-N252P-K312Q、S225G-N260Q-H272V、S225G-N260Q-S279D、S225G-N260Q-Y281P、S225G-N260Q-K308Q、S225G-N260Q-K309E、S225G-N260Q-K312Q、S225G-H272V-S279D、S225G-H272V-Y281P、S225G-H272V-K308Q、S225G-H272V-K309E、S225G-H272V-K312Q、S225G-S279D-Y281P、S225G-S279D-K308Q、S225G-S279D-K309E、S225G-S279D-K312Q、S225G-Y281P-K308Q、S225G-Y281P-K309E、S225G-Y281P-K312Q、S225G-K308Q-K309E、S225G-K308Q-K312Q、S225G-K309E-K312Q、S225G-K308Q-K309E-K312Q、<h2 style=";text-align:left;direction:ltr">S225G-N227T-N252P-S186R-N260Q-K308E-K309E-K312E、N227T-N252P、N 227T-N267T、N227T-F276A、N227T-Y281P、N227T-K308Q、N227T-K308E、N22 7T-K309E、N227T-K312Q、N227T-K312E、N227T-E232D、N227T-G235W、N227T -N260Q、N227T-H272V、N227T-S279D、N227T-E232D-G235W、N227T-E232D-N 252P、N227T-E232D-N260Q、N227T-E232D-H272V、N227T-E232D-S279D、N227T-E232D-Y281P、N227T-E232D-K308Q、N227T-E232D-K309E、N227T-E232D -K312Q、N227T-G235W-N252P、N227T-G235W-N260Q、N227T-G235W-H272V、N 227T-G235W-S279D、N227T-G235W-Y281P、N227T-G235W-K308Q、N227T-G23 5W-K309E、N227T-G235W-K312Q、N227T-N252P-N260Q、N227T-N252P-H272V、N227T-N252P-S279D、N227T-N252P-Y281P、N227T-N252P-K308Q、N227T- N252P-K309E、N227T-N252P-K312Q、N227T-N260Q-H272V、N227T-N260Q-S279D、N227T-N260Q-Y281P、N227T-N260Q-K308Q、N227T-N260Q-K309E、N227 T-N260Q-K312Q、N227T-H272V-S279D、N227T-H272V-Y281P、N227T-H272V- K308Q、N227T-H272V-K309E、N227T-H272V-K312Q、N227T-S279D-Y281P、N2 27T-S279D-K308Q、N227T-S279D-K309E、N227T-S279D-K312Q、N227T-Y281P-K308Q、N227T-Y281P-K309E、N227T-Y281P-K312Q、N227T-K308Q-K309E、N227T-K308Q-K312Q、N227T-K309E-K312Q、N227T-N252P-K308E、N227T-N252P-K312E、N227T-N252P-N267T、N227T-N267T-F276A、N227T-N267T-K308E、N227T-N267T-K309E、N227T-N267T-K312E、N227T-F276A-K308E、N227T-F276A-K309E、N227T-F276A-K312E、N227T-K308E-K309E、N227T-K308E-K312E、N227T-K309E-K312E、N227T-F276A-K308E-K309E、N227T-F276A-K308E-K312E、N227T-F276A-K309E-K312E、N227T-K308E-K309E-K312E、N227T-S225G-K308Q-K309E、N227T-S225G-K308Q-K312Q、N227T-S225G-K309E-K312Q、N227T-K308Q-K309E-K312Q、N227T-N267T-F276A-K308E、N227T-N267T-F276A-K309E、N227T-N267T-F276A-K312E、N227T-N267T-S225G-K308Q、N227T-N267T-S225G-K309E、N227T-N267T-S225G-K312Q、N227T-N252P-N267T-F276A-K308E、N227T-N252P-N267T-F276A-K309E、N227T-N252P-N267T-K308E-K309E、N227T-N252P-F276A-K308E-K309E、N227T-N267T-F276A-K308E-K309E、N227T-F276A-K308E-K309E-K312E、N227T-N252P-E232D-S225G-K308Q、N227T-N252P-E232D-S225G-K309E、N227T-N252P-E232D-K308Q-K309E、N227T-N252P-S225G-K308Q-K309E、N227T-E232D-S225G-K308Q-K309E、N227T-S225G-K308Q-K309E-K312Q、N227T-N267T-F276A-K308E-K312E、<h2 style=";text-align:left;direction:ltr">N227T-N267T-F276A-K309E-K312E、N227T-E232D-S225G-K308Q-K312Q、N 227T-E232D-S225G-K309E-K312Q、N227T-N267T-F276A-K308E-K309E-K31 2E、N227T-N252P-F276A-K308E-K309E-K312E、N227T-S225G-S186R-K308Q-K309E-K312Q、N227T-N252P-S186R-K308Q-K309E-K312Q、N227T-N252P- N267T-F276A-K308E-K309E-K312E、N227T-N252P-S225G-S186R-K308Q-K 309E-K312Q、E232D-G235W、E232D-N252P、E232D-N260Q、E232D-H272V、E23 2D-S279D、E232D-Y281P、E232D-K308Q、E232D-K309E、E232D-K312Q、E232D-G235W-N252P、E232D-G235W-N260Q、E232D-G235W-H272V、E232D-G235W- S279D、E232D-G235W-Y281P、E232D-G235W-K308Q、E232D-G235W-K309E、E 232D-G235W-K312Q、E232D-N252P-N260Q、E232D-N252P-H272V、E232D-N25 2P-S279D、E232D-N252P-Y281P、E232D-N252P-K308Q、E232D-N252P-K309E、E232D-N252P-K312Q、E232D-N260Q-H272V、E232D-N260Q-S279D、E232D- N260Q-Y281P、E232D-N260Q-K308Q、E232D-N260Q-K309E、E232D-N260Q-K312Q、E232D-H272V-S279D、E232D-H272V-Y281P、E232D-H272V-K308Q、E23 2D-H272V-K309E、E232D-H272V-K312Q、E232D-S279D-Y281P、E232D-S279D-K308Q、E232D-S279D-K309E、E232D-S279D-K312Q、E232D-Y281P-K308Q、E232D-Y281P-K309E、E232D-Y281P-K312Q、E232D-K308Q-K309E、E232D-K308Q-K312Q、E232D-K309E-K312Q、G235W-N252P、G235W-N260Q、G235W-H272V、G235W-S279D、G235W-Y281P、G235W-K308Q、G235W-K309E、G235W-K312Q、G235W-N252P-N260Q、G235W-N252P-H272V、G235W-N252P-S279D、G235W-N252P-Y281P、G235W-N252P-K308Q、G235W-N252P-K309E、G235W-N252P-K312Q、G235W-N260Q-H272V、G235W-N260Q-S279D、G235W-N260Q-Y281P、G235W-N260Q-K308Q、G235W-N260Q-K309E、G235W-N260Q-K312Q、G235W-H272V-S279D、G235W-H272V-Y281P、G235W-H272V-K308Q、G235W-H272V-K309E、G235W-H272V-K312Q、G235W-S279D-Y281P、G235W-S279D-K308Q、G235W-S279D-K309E、G235W-S279D-K312Q、G235W-Y281P-K308Q、G235W-Y281P-K309E、G235W-Y281P-K312Q、G235W-K308Q-K309E、G235W-K308Q-K312Q、G235W-K309E-K312Q、G235W-N227T-S186R-K308Q-K309E-K312Q、G235W-N227T-N252P-S186R-N260Q-K308Q-K309E-K312Q、N252P-N267T、N252P-F276A、N252P-Y281P、N252P-K308Q、N252P-K308E、N252P-K309E、N252P-K312Q、N252P-K312E、N252P-N260Q、N252P-H272V、N252P-S279D、N252P-N260Q-H272V、N252P-N260Q-S279D、N252P-N260Q-Y281P、N252P-N260Q-K308Q、N252P-N260Q-K309E、<h2 style=";text-align:left;direction:ltr">N252P-N260Q-K312Q、N252P-H272V-S279D、N252P-H272V-Y281P、N252P-H272V-K308Q、N252P-H272V-K309E、N252P-H272V-K312Q、N252P-S279D-Y281 P、N252P-S279D-K308Q、N252P-S279D-K309E、N252P-S279D-K312Q、N252P- Y281P-K308Q、N252P-Y281P-K309E、N252P-Y281P-K312Q、N252P-K308Q-K30 9E、N252P-K308Q-K312Q、N252P-K309E-K312Q、N252P-F276A-K308E、N252P -F276A-K309E、N252P-F276A-K312E、N252P-K308E-K309E、N252P-K308E-K 312E、N252P-N267T-F276A、N252P-N267T-K308E、N252P-N267T-K309E、N252P-N267T-K312E、N252P-N267T-F276A-K308E-K309E、N252P-E232D-S225G- K308Q-K309E、N260Q-H272V、N260Q-S279D、N260Q-Y281P、N260Q-K308Q、N2 60Q-K309E、N260Q-K312Q、N260Q-H272V-S279D、N260Q-H272V-Y281P、N260 Q-H272V-K308Q、N260Q-H272V-K309E、N260Q-H272V-K312Q、N260Q-S279D- Y281P、N260Q-S279D-K308Q、N260Q-S279D-K309E、N260Q-S279D-K312Q、N26 0Q-Y281P-K308Q、N260Q-Y281P-K309E、N260Q-Y281P-K312Q、N260Q-K308Q -K309E、N260Q-K308Q-K312Q、N260Q-K309E-K312Q、N267T-F276A、N267T-Y 281P、N267T-K308E、N267T-K309E、N267T-K312Q、N267T-K312E、N267T-F27 6A-K308E、N267T-F276A-K309E、N267T-F276A-K312E、N267T-K308E-K309E、<h2 style=";text-align:left;direction:ltr">N267T-K308E-K312E、N267T-K309E-K312E、H272V-S279D、H272V-Y281P、H272V-K308Q、H272V-K309E、H272V-K312Q、H272V-S279D-Y281P、H272V-S279 D-K308Q、H272V-S279D-K309E、H272V-S279D-K312Q、H272V-Y281P-K308Q、H272V-Y281P-K309E、H272V-Y281P-K312Q、H272V-K308Q-K309E、H272V-K30 8Q-K312Q、H272V-K309E-K312Q、F276A-Y281P、F276A-K308Q、F276A-K308E 、F276A-K309E、F276A-K312Q、F276A-K312E、F276A-K308E-K309E、F276A-K 308E-K312E、F276A-K309E-K312E、F276A-K308E-K309E-K312E、S279D-Y281P、S279D-K308Q、S279D-K309E、S279D-K312Q、S279D-Y281P-K308Q、S279D- Y281P-K309E、S279D-Y281P-K312Q、S279D-K308Q-K309E、S279D-K308Q-K312Q、S279D-K309E-K312Q、Y281P-K308Q、Y281P-K308E、Y281P-K309E、Y281 P-K312Q、Y281P-K312E、Y281P-K308Q-K309E、Y281P-K308Q-K312Q、Y281P- K309E-K312Q、K308E-K312Q、K308E-K312E、K308Q-K312Q、K309E-K312Q、K30 9E-K312E、K308E-K309E、K308Q-K309E、K308Q-K309E-K312Q、K308E-K309E -K312E、K312E-N227T-F276A、K312E-N227T-K308E、K312E-N227T-K309E、K 312E-N227T-K312E、K312E-F276A-K308E、K312E-F276A-K309E、K312E-F276A-K312E、K312E-K308E-K309E、K312E-K308E-K312E、K312E-K309E-K312E、K312E-N227T-F276A-K308E、K312E-N227T-F276A-K309E、K312E-N227T-F276A-K312E、K312E-N227T-K308E-K309E、K312E-N227T-K308E-K312E、K312E-N227T-K309E-K312E、K312E-F276A-K308E-K309E、K312E-F276A-K308E-K312E、K312E-F276A-K309E-K312E、K312E-K308E-K309E-K312E、K312Q-N227T-S225G-K308Q、K312Q-N227T-S225G-K309E、K312Q-N227T-S225G-K312Q、K312Q-N227T-K308Q-K309E、K312Q-N227T-K308Q-K312Q、K312Q-N227T-K309E-K312Q、K312Q-S225G-K308Q-K309E、K312Q-S225G-K308Q-K312Q、K312Q-S225G-K309E-K312Q、K312Q-K308Q-K309E-K312Q。、

[0044] In a further preferred embodiment, a dispersin having β-1,6-N-acetylglucosaminidase activity is used and comprises an amino acid sequence that is at least 70% (and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96% or 96.5%) identical over its entire length to the amino acid sequence given in SEQ ID NO: 1 and having at least ten, and increasingly preferably eleven, twelve, 13, 14, 15, 16, 17, 18 or more amino acid substitutions, wherein the dispersin is based on the sequence according to SEQ ID NO: The numbering of NO: 1 has an amino acid substitution combination selected from the following: Q3F-A49W-N59E-S163P-S186R-S225G-N227T-N252P-F276A-Y281P-K308E-K309E-K312E, Q3F-A49W-N59E-S163P-S186R-N227T-E232D-N252P-F276A-S279G-K308E-K309E-K312E 09E-K312E, Q3F-T17W-A49W-N59E-S163P-S186R-S225G-N227T-G235W-N252P-H272P-Y281P-K30 8Q-K309E-K312Q, Q3F-A49W-N59E-S163P-S186R-S225G-N227T-N252P-F276A-S279G-K308E-K30 9E-K312E, Q3F-A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P-F276A-S279G-K308E-K30 9E-K312E, Q3F-S163P-S186R-Q215K-N227T-E232D-N252P-N267T-F276A-S279G-K308E-K309E-K 312E, Q3F-S163P-S186R-Q215K-S225G-N227T-N252P-N267T-F276A-S279G-K308E-K309E-K312E , Q3F-A49W-N59E-S163P-S186R-N227T-E232D-N252P-F276A-S279G-Y281P-K308E-K309E-K312E,Q3F-T17W-A49W-N59E-S163P-S186R-D207N-T218Q-S225G-N227T-G235W-N252P-H272P-Y281P-K308Q-K309E-K312Q、Q3F-A49W-N59E-S163P-S186R-S225G-N227T-N252P-F276A-S279G-Y281P-K308E-K309E-K312E、Q3F-A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P-H272V-F276A-S279G-K308E-K309E-K312E、Q3F-H15Y-V140I-S163P-S186R-Q215K-S225G-N227T-E232D-G235W-N252P-N260Q-N267T-H272V-S279D-Y281P-K308Q-K309E-K312Q、Q3F-H15Y-S163P-S186R-Q215K-S225G-N227T-E232D-N252P-N260Q-N267T-H272V-S279D-Y281P-K308Q-K309E-K312Q、Q3F-H15Y-T17W-A49W-N59E-S163P-S186R-T218Q-S225G-N227T-G235W-S237W-N252P-H272P-K308Q-K309E-K312Q、Q3F-H15Y-T17W-A49W-N59E-S163P-S186R-D207N-T218Q-S225G-N227T-G235W-S237W-N252P-H272P-Y281P-K308Q-K309E-K312Q、Q3F-S163P-S186R-Q215K-N227T-E232D-N252P-N267T-H272V-F276A-S279G-K308E-K309E-K312E、Q3F-S163P-S186R-Q215K-N227T-E232D-N252P-N267T-F276A-S279G-Y281P-K308E-K309E-K312E、Q3F-S163P-Q215K-N227T-E232D-N252P-N267T-F276A-S279G-Y281P-K308E-K309E-K312E、Q3F-H15Y-T17W-A49W-N59E-S163P-S186R-T218Q-S225G-N227T-G235W-S237W-N252P-H272P-Y281P-K308Q-K309E-K312Q、Q3F-S163P-Q215K-S225G-N227T-E232D-N252P-N267T-H272V-F276A-S279G-K308E-K309E-K312E、Q3F-A49W-N59E-S163P-S186R-N227T-E232D-N252P-H272V-F276A-S279G-Y281P-K308E-K309E-K312E、Q3F-S163P-S186R-Q215K-S225G-N227T-N252P-N267T-F276A-S279G-Y281P-K308E-K309E-K312E、Q3F-S163P-Q215K-N227T-E232D-N252P-N267T-H272V-F276A-S279G-Y281P-K308E-K309E-K312E、Q3F-A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P-F276A-S279G-Y281P-K308E-K309E-K312E、Q3F-A49W-N59E-S163P-S186R-S225G-N227T-N252P-H272V-F276A-S279G-Y281P-K308E-K309E-K312E、Q3F-S163P-S186R-Q215K-S225G-N227T-E232D-N252P-N260Q-N267T-H272P-S279D-Y281P-K308Q-K309E-K312Q、Q3F-S163P-S186R-Q215K-N227T-E232D-N252P-N267T-H272V-F276A-S279G-Y281P-K308E-K309E-K312E、Q3F-S163P-S186R-Q215K-S225G-N227T-N252P-N267T-H272V-F276A-S279G-Y281P-K308E-K309E-K312E、Q3F-T17W-S163P-S186R-Q215K-S225G-N227T-E232D-G235W-N252P-N267T-H272V-F276A-S279G-Y281P-K308E-K309E-K312Q、Q3F-S163P-S186R-Q215K-S225G-N227T-E232D-N252P-N267T-H272V-F276A-S279G-Y281P-K308E-K309E-K312Q、Q3F-A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P-H272V-F276A-S279G-Y281P-K308E-K309E-K312E、Q3F-S163P-S186R-Q215K-S225G-N227T-E232D-N252P-N267T-H272V-F276A-S279G-Y281P-K308E-K309E-K312E、Q3F-H15Y-T17W-S163P-S186R-Q215K-S225G-N227T-E232D-G235W-N252P-N260Q-N267T-H272V-S279D-Y281P-K308Q-K309E-K312Q、Q3F-S163P-Q215K-S225G-N227T-E232D-N252P-N267T-H272V-F276A-S279G-Y281P-K308E-K309E-K312E、Q3F-H15Y-S163P-S186R-Q215K-S225G-N227T-E232D-G235W-N252P-N260Q-N267T-H272P-S279D-Y281P-K308Q-K309E-K312Q、Q3F-A49W-N59E-S163P-S186R-S225G-N227T-E232D-G235W-N252P-N260Q-H272P-F276A-S279D-Y281P-K308E-K309E-K312Q、Q3F-H15Y-T17W-S163P-S186R-Q215K-S225G-N227T-E232D-N252P-N260Q-N267T-H272V-S279D-Y281P-K308Q-K309E-K312Q、Q3F-H15Y-S163P-S186R-Q215K-S225G-N227T-E232D-N252P-N260Q-N267T-H272P-S279D-Y281P-K308Q-K309E-K312Q、Q3F-H15Y-S163P-S186R-Q215K-S225G-N227T-E232D-G235W-N252P-N267T-H272V-F276A-S279G-Y281P-K308Q-K309E-K312Q、<h2 style=";text-align:left;direction:ltr">Q3F-H15Y-T17W-S163P-S186R-Q215K-S225G-N227T-E232D-N252P-N267 T-H272V-F276A-S279G-Y281P-K308Q-K309E-K312Q、Q3F-H15Y-A49W-N59 E-V140I-S163P-S186R-Q215K-S225G-N227T-E232D-G235W-N252P-N260 Q-N267T-H272V-S279D-Y281P-K308Q-K309E-K312Q、Q3I-A49W-N59E-S16 3P-S186R-S225G-N227T-Y281P-K308Q-K309E-K312Q、Q3I-A49W-N59E-S163P-S186R-S225G-E232D-Y281P-K308Q-K309E-K312Q、Q3I-A49W-N59E- S163P-S186R-S225G-G235W-Y281P-K308Q-K309E-K312Q、Q3I-A49W-N59 E-S163P-S186R-S225G-N252P-Y281P-K308Q-K309E-K312Q、Q3I-A49W-N5 9E-S163P-S186R-S225G-N260Q-Y281P-K308Q-K309E-K312Q、Q3I-A49W- N59E-S163P-S186R-S225G-N227T-H272V-Y281P-K308Q-K309E、Q3I-A49W -N59E-S163P-S186R-S225G-N227T-S279D-Y281P-K308Q-K312Q、Q3I-H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P-N260Q-N267T-H272V-S279D-Y281P-K308Q-K309E-K312Q、Q3I-H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P-N260Q-N267T-H272P-S279D-Y281P- K308Q-K309E-K312Q、Q3I-H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-G235W-N252P-N260Q-H272V-S279D-Y281P-K308Q-K309E-K312E、Q3I-H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-G235W-N252P-N260Q-H272V-S279D-Y281P-K308E-K309E-K312Q、Q3I-H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-G235W-N252P-N260Q-H272V-S279D-Y281P-K308Q-K309E-K312Q、H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-Y281P-K309E-K312Q、H15Y-A49W-N59E-S163P-S186R-S225G-N227T-G235W-K308Q-K309E-K312Q、H15Y-A49W-S163P-S186R-S225G-N227T-Y281P-S288P-K308Q-K309E-K312Q、H15Y-A49W-S163P-S186R-S225G-N227T-N252P-Y281P-K308Q-K309E-K312Q、H15Y-A49W-S163P-S186R-S225G-N227T-H272P-Y281P-K308Q-K309E-K312Q、H15Y-A49W-S163P-S186R-S225G-N227T-S279D-Y281P-K308Q-K309E-K312Q、H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-Y281P-K308Q-K312Q、H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-G235W-N252P-N260Q-H272P-S279D-Y281P-K308Q-K309E-K312Q、H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-G235W-N252P-N260Q-H272V-S279D-Y281P-K308Q-K309E-K312Q、H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P-N260Q-H272V-Y281P-K308Q-K309E-K312Q、H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-G235W-N252P-N260Q-H272V-S279D-Y281P-K309E-K312Q、H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P-N260Q-H272P-S279D-Y281P-S288P-K308Q-K309E-K312Q、H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P-N260Q-H272P-S279D-Y281P-K308Q-K309E-K312Q、H15Y-T17W-A49W-N59E-S163P-S186R-S225G-N227T-E232D-G235W-N252P-N260Q-H272V-S279D-Y281P-K308Q-K309E-K312Q、H15Y-T17W-A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P-N260Q-H272V-S279D-Y281P-K308Q-K309E-K312Q、A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P-F276A-S279G-K308E-K309E-K312E、A49W-N59E-S163P-S186R-N227T-E232D-N252P-H272V-F276A-S279G-K308E-K309E-K312E、A49W-N59E-S163P-S186R-N227T-E232D-N252P-F276A-S279G-Y281P-K308E-K309E-K312E、A49W-N59E-S163P-S186R-S225G-N227T-N252P-F276A-S279G-Y281P-K308E-K309E-K312E、A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P-H272V-F276A-S279G-K308E-K309E-K312E、A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P-F276A-S279G-Y281P-K308E-K309E-K312E、A49W-N59E-S163P-S186R-N227T-E232D-N252P-H272V-F276A-S279G-Y281P-K308E-K309E-K312E、A49W-N59E-S163P-S186R-S225G-N227T-N252P-H272V-F276A-S279G-Y281P-K308E-K309E-K312E、A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P-H272V-F276A-S279G-Y281P-K308E-K309E-K312E、S163P-S186R-Q215K-N227T-E232D-N252P-N267T-F276A-S279G-Y281P-K308E-K309E-K312E、S163P-S186R-S225G-N227T-E232D-N252P-N267T-F276A-S279G-Y281P-K308E-K309E-K312E、S163P-S186R-Q215K-S225G-N227T-E232D-N252P-N267T-F276A-S279G-Y281P-K308E-K309E-K312E、S163P-Q215K-S225G-N227T-E232D-N252P-N267T-F276A-S279G-Y281P-K308E-K309E-K312E、S163P-S186R-Q215K-S225G-N227T-E232D-N252P-N267T-H272V-F276A-S279G-K308E-K309E-K312E、S163P-Q215K-S225G-N227T-E232D-N252P-N267T-H272V-F276A-S279G-K308E-K309E-K312E、S163P-Q215K-N227T-E232D-N252P-N267T-H272V-F276A-S279G-Y281P-K308E-K309E-K312E、S163P-S186R-Q215K-S225G-N227T-N252P-N267T-H272V-F276A-S279G-Y281P-K308E-K309E-K312E、S163P-Q215K-S225G-N227T-N252P-N267T-H272V-F276A-S279G-Y281P-K308E-K309E-K312E、S163P-Q215K-S225G-N227T-E232D-N252P-N267T-H272V-F276A-S279G-Y281P-K308E-K309E-K312E and S163P-S186R-Q215K-S225G-N227T-E232D-N252P-N267T-H272V-F276A-S279G-Y281P-K308E-K309E-K312E.

[0045] In a particularly preferred embodiment, a dispersin having β-1,6-N-acetylglucosaminidase activity is used and which comprises an amino acid sequence that is at least 70% (and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94% or 95%) identical over its entire length to the amino acid sequence given in SEQ ID NO: 1 and having at least 15, and increasingly preferably 16, 17, 18 or 19 amino acid substitutions, wherein the dispersin is based on the amino acid sequence given in SEQ ID NO: 1. The numbering of NO: 1 has an amino acid substitution combination selected from the following: Q3F-T17W-A49W-N59E-S163P-S186R-S225G-N227T-G235W-N252P-H272P-Y281P-K308Q-K309E-K312Q, Q3F-T17W-A49W-N59E-S163P-S186R-D207N-T218Q-S225G-N227T-G235W-N252P-H2 72P-Y281P-K308Q-K309E-K312Q, Q3F-A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P-H272V-F276 A-S279G-K308E-K309E-K312E, Q3F-H15Y-V140I-S163P-S186R-Q215K-S225G-N227T-E232D-G235W-N252P -N260Q-N267T-H272V-S279D-Y281P-K308Q-K309E-K312Q, Q3F-H15Y-S163P-S186R-Q215K-S225G-N227T- E232D-N252P-N260Q-N267T-H272V-S279D-Y281P-K308Q-K309E-K312Q, Q3F-H15Y-T17W-A49W-N59E-S163 P-S186R-T218Q-S225G-N227T-G235W-S237W-N252P-H272P-K308Q-K309E-K312Q, Q3F-H15Y-T17W-A49W-N 59E-S163P-S186R-D207N-T218Q-S225G-N227T-G235W-S237W-N252P-H272P-Y281P-K308Q-K309E-K312Q,Q3F-H15Y-T17W-A49W-N59E-S163P-S186R-T218Q-S225G-N227T-G235W-S237W-N252P-H272P-Y281P-K308Q-K309E-K312Q、Q3F-A49W-N59E-S163P-S186R-N227T-E232D-N252P-H272V-F276A-S279G-Y281P-K308E-K309E-K312E、Q3F-A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P-F276A-S279G-Y281P-K308E-K309E-K312E、Q3F-A49W-N59E-S163P-S186R-S225G-N227T-N252P-H272V-F276A-S279G-Y281P-K308E-K309E-K312E、Q3F-S163P-S186R-Q215K-S225G-N227T-E232D-N252P-N260Q-N267T-H272P-S279D-Y281P-K308Q-K309E-K312Q、Q3F-S163P-S186R-Q215K-N227T-E232D-N252P-N267T-H272V-F276A-S279G-Y281P-K308E-K309E-K312E、Q3F-S163P-S186R-Q215K-S225G-N227T-N252P-N267T-H272V-F276A-S279G-Y281P-K308E-K309E-K312E、Q3F-T17W-S163P-S186R-Q215K-S225G-N227T-E232D-G235W-N252P-N267T-H272V-F276A-S279G-Y281P-K308E-K309E-K312Q、Q3F-S163P-S186R-Q215K-S225G-N227T-E232D-N252P-N267T-H272V-F276A-S279G-Y281P-K308E-K309E-K312Q、Q3F-A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P-H272V-F276A-S279G-Y281P-K308E-K309E-K312E、Q3F-S163P-S186R-Q215K-S225G-N227T-E232D-N252P-N267T-H272V-F276A-S279G-Y281P-K308E-K309E-K312E、Q3F-H15Y-T17W-S163P-S186R-Q215K-S225G-N227T-E232D-G235W-N252P-N260Q-N267T-H272V-S279D-Y281P-K308Q-K309E-K312Q、Q3F-S163P-Q215K-S225G-N227T-E232D-N252P-N267T-H272V-F276A-S279G-Y281P-K308E-K309E-K312E、Q3F-H15Y-S163P-S186R-Q215K-S225G-N227T-E232D-G235W-N252P-N260Q-N267T-H272P-S279D-Y281P-K308Q-K309E-K312Q、Q3F-A49W-N59E-S163P-S186R-S225G-N227T-E232D-G235W-N252P-N260Q-H272P-F276A-S279D-Y281P-K308E-K309E-K312Q、Q3F-H15Y-T17W-S163P-S186R-Q215K-S225G-N227T-E232D-N252P-N260Q-N267T-H272V-S279D-Y281P-K308Q-K309E-K312Q、Q3F-H15Y-S163P-S186R-Q215K-S225G-N227T-E232D-N252P-N260Q-N267T-H272P-S279D-Y281P-K308Q-K309E-K312Q、Q3F-H15Y-S163P-S186R-Q215K-S225G-N227T-E232D-G235W-N252P-N267T-H272V-F276A-S279G-Y281P-K308Q-K309E-K312Q、Q3F-H15Y-T17W-S163P-S186R-Q215K-S225G-N227T-E232D-N252P-N267T-H272V-F276A-S279G-Y281P-K308Q-K309E-K312Q、<h2 style=";text-align:left;direction:ltr">Q3F-H15Y-A49W-N59E-V140I-S163P-S186R-Q215K-S225G-N227T-E232D-G235W-N252P-N260Q-N267T-H272V-S279D-Y281P-K308Q-K309E-K312Q、Q3I-H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P-N260Q-N267T-H272V-S279D-Y281P-K308Q-K309E-K312Q、Q3I-H1 5Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P-N260Q-N267T-H272P-S279D-Y281P-K308Q-K309E-K312Q、Q3I-H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-G235W-N252P-N260Q-H272V-S279D-Y281P-K308Q-K309E-K312E、Q3I-H15Y-A49W-N59E-S163P-S186R -S225G-N227T-E232D-G235W-N252P-N260Q-H272V-S279D-Y281P-K3 08E-K309E-K312Q、Q3I-H15Y-A49W-N59E-S163P-S186R-S225G-N227T -E232D-G235W-N252P-N260Q-H272V-S279D-Y281P-K308Q-K309E-K3 12Q、H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-G235W-N25 2P-N260Q-H272P-S279D-Y281P-K308Q-K309E-K312Q、H15Y-A49W-N5 9E-S163P-S186R-S225G-N227T-E232D-G235W-N252P-N260Q-H272V-S 279D-Y281P-K308Q-K309E-K312Q、H15Y-A49W-N59E-S163P-S186R-S2 25G-N227T-E232D-N252P-N260Q-H272V-Y281P-K308Q-K309E-K312Q、H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-G235W-N252P-N260Q-H272V-S279D-Y281P-K3 09E-K312Q, H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P-N260Q-H272P-S279D-Y281 P-S288P-K308Q-K309E-K312Q, H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P-N260Q- H272P-S279D-Y281P-K308Q-K309E-K312Q, H15Y-T17W-A49W-N59E-S163P-S186R-S225G-N227T-E23 2D-G235W-N252P-N260Q-H272V-S279D-Y281P-K308Q-K309E-K312Q, H15Y-T17W-A49W-N59E-S163P- S186R-S225G-N227T-E232D-N252P-N260Q-H272V-S279D-Y281P-K308Q-K309E-K312Q, A49W-N59E-S 163P-S186R-S225G-N227T-E232D-N252P-H272V-F276A-S279G-Y281P-K308E-K309E-K312E and S163P-S186R-Q215K-S225G-N227T-E232D-N252P-N267T-H272V-F276A-S279G-Y281P-K308E-K309E-K312E.

[0046] In a very particularly preferred embodiment, a dispersin having β-1,6-N-acetylglucosaminidase activity is used and which comprises an amino acid sequence that is at least 70% (and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93% or 94%) identical over its entire length to the amino acid sequence given in SEQ ID NO: 1 and has at least 18 amino acid substitutions, wherein the dispersin is based on the amino acid sequence given in SEQ ID NO: 1. The numbering of NO: 1 has an amino acid substitution combination selected from the following: Q3F-H15Y-V140I-S163P-S186R-Q215K-S225G-N227T-E232D-G235W-N252P-N260Q-N267T-H272V-S279D-Y281P-K308Q-K309E-K312Q, Q3F-H15Y-T17W-A49W-N59E-S163P-S186R-D207N-T218Q-S225G-N227 T-G235W-S237W-N252P-H272P-Y281P-K308Q-K309E-K312Q, Q3F-H15Y-T17W-A49W-N59E-S163P-S186R-T218Q-S225 G-N227T-G235W-S237W-N252P-H272P-Y281P-K308Q-K309E-K312Q, Q3F-T17W-S163P-S186R-Q215K-S225G-N227T-E 232D-G235W-N252P-N267T-H272V-F276A-S279G-Y281P-K308E-K309E-K312Q, Q3F-H15Y-T17W-S163P-S186R-Q215K -S225G-N227T-E232D-G235W-N252P-N260Q-N267T-H272V-S279D-Y281P-K308Q-K309E-K312Q, Q3F-H15Y-S163P-S1 86R-Q215K-S225G-N227T-E232D-G235W-N252P-N260Q-N267T-H272P-S279D-Y281P-K308Q-K309E-K312Q, Q3F-A49W -N59E-S163P-S186R-S225G-N227T-E232D-G235W-N252P-N260Q-H272P-F276A-S279D-Y281P-K308E-K309E-K312Q,Q3F-H15Y-T17W-S163P-S186R-Q215K-S225G-N227T-E232D-N252P-N260Q-N267T-H272V-S279D-Y281P-K308Q-K309E-K312Q、Q3F-H15Y-S163P-S186R-Q215K-S225G-N227T-E232D-G235W-N252P-N267T-H272V-F276A-S279G-Y281P-K308Q-K309E-K312Q、Q3F-H15Y-T17W-S163P-S186R-Q215K-S225G-N227T-E232D-N252P-N267T-H272V-F276A-S279G-Y281P-K308Q-K309E-K312Q、Q3F-H15Y-A49W-N59E-V140I-S163P-S186R-Q215K-S225G-N227T-E232D-G235W-N252P-N260Q-N267T-H272V-S279D-Y281P-K308Q-K309E-K312Q、Q3I-H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P-N260Q-N267T-H272V-S279D-Y281P-K308Q-K309E-K312Q、Q3I-H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P-N260Q-N267T-H272P-S279D-Y281P-K308Q-K309E-K312Q、Q3I-H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-G235W-N252P-N260Q-H272V-S279D-Y281P-K308Q-K309E-K312E、Q3I-H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-G235W-N252P-N260Q-H272V-S279D-Y281P-K308E-K309E-K312Q、Q3I-H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-G235W-N252P-N260Q-H272V-S279D-Y281P-K308Q-K309E-K312Q and H15Y-T17W-A49W-N59E-S163P-S186R-S225G-N227T-E232D-G235W-N252P-N260Q-H272V-S279D-Y281P-K308Q-K309E-K312Q.

[0047] As used herein, "β-1,6-N-acetylglucosaminidase activity" is synonymous with "hexosaminidase activity" and / or "dispersin activity" and is determined as follows: To determine dispersin activity, 35 μl of dispersin assay solution (45 mM citrate buffer, pH 5, containing 0.5 mg / ml p-nitrophenyl-N-acetyl-β-D-glucosaminidase) is added to each well of a microplate containing 5 μl of sample solution. The microplate is incubated at room temperature for 3 hours. After incubation, 40 μl of stop solution (0.4 M Na2CO3) is added, and the absorbance is measured at 405 nm. The activity values ​​obtained are corrected by subtracting the absorbance measurement of a reference sample without dispersin to eliminate background noise. From this, the residual activity (RA) of the corresponding dispersin variant relative to the reference dispersin can be calculated.

[0048] The enzymes mentioned herein are preferably mature enzymes, ie catalytically active molecules without signal peptides and / or propeptides. Unless otherwise indicated, the indicated sequences in each case also refer to the mature (processed) enzyme.

[0049] In various embodiments of the present invention, corresponding enzyme is free available enzyme.This means that this enzyme can directly interact with all components of described reagent, and if described reagent is liquid reagent, then this enzyme directly contacts the solvent (for example water) of described reagent.In other embodiments, reagent can contain the enzyme that forms interaction complex with other molecules or contain " parcel ".In this case, single enzyme molecule or multiple enzyme molecules can be separated from other components of reagent by surrounding structure.This type of separation structure can be derived from but not limited to vesicle, as micelle or liposome.However, surrounding structure also can be virus particle, bacterial cell or eukaryotic cell.In various embodiments, reagent can comprise the cell of Bacillus pumilus (Bacillus pumilus) or Bacillus subtilis (Bacillus subtilis) of expression according to proteolytic enzyme of the present invention, or the cell culture supernatant of this type of cell.

[0050] In the context of the present invention, the feature means that the enzyme has the indicated substitution (or deletion or insertion), i.e. it contains one (one of the indicated) substitution (or deletion or insertion) at the corresponding position, i.e. at least the indicated position has not been mutated or deleted in another way, for example by fragmentation of the enzyme. In various embodiments, the enzymes described herein have the sequence of the respective reference sequence except for the explicitly mentioned substitutions, i.e. are 100% identical to the respective reference sequence except for the substitution positions.

[0051] The homogeneity of nucleic acid or amino acid sequence is determined by sequence alignment. This sequence alignment is based on the BLAST algorithm set up and commonly used in the prior art (see, for example, Altschul et al., " Basic local alignment search tool, " J.Mol.Biol., 1990, 215: 403-410, and Altschul et al., " Gapped BLAST and PSI-BLAST: a newgeneration of protein database search programs, " Nucleic Acids Res., 1997, 25: 3389-3402), and its principle is that similar nucleotides or amino acid sequences in nucleic acid sequence or amino acid sequence are distributed to each other. The table distribution of related positions is called comparison. Another algorithm available in the prior art is the FASTA algorithm. Sequence alignment (alignment), particularly multiple sequence alignment, is created using a computer program. Commonly used are the Clustal series (see, for example, Chenna et al., "Multiple sequence alignment with the Clustal series of programs," Nucleic Acid Res., 2003, 31:3497-3500), T-Coffee (see, for example, Notredame et al., "T-Coffee: A novel method for multiple sequence alignments," J. Mol. Biol., 2000, 302:205-217) or programs based on these programs or algorithms. The computer program Vector Suite 10.3 (Invitrogen Corporation, 1600 Faraday Avenue, Carlsbad, California, USA) was used for sequence alignment using its specified standard parameters. The AlignX module for sequence alignment was based on ClustalW; or Clone Manager 10 was used (sequence alignment was performed using the scoring matrix BLOSUM 62 at the amino acid level). Unless otherwise stated, sequence identities described herein were determined using the BLAST algorithm.

[0052] This comparison also allows inference of the similarity between the compared sequences. It is usually given as percentage identity, i.e., the ratio of identical nucleotides or amino acid residues in the same position or corresponding positions in the alignment. For amino acid sequences, the broader concept of homology takes into account conservative amino acid substitutions, i.e., amino acids with similar chemical activities, because they generally perform similar chemical activities within the protein. Therefore, the similarity of the compared sequences can also be expressed as percentage identity or percentage similarity. The identity and / or homology information provided can relate to the entire polypeptide or gene, or only to individual regions. Therefore, the homology or identical regions of different nucleic acid or amino acid sequences are defined by the matches in the sequence. These regions generally have the same function. They may be very small and contain only a few nucleotides or amino acids. Typically, such small regions perform essential functions for the overall activity of the protein. Therefore, it may be advantageous to associate sequence matches only to single, optional small regions. However, unless otherwise stated, the identity or homology information in this application relates to the entire length of the indicated specific nucleic acid or amino acid sequence.

[0053] In the context of the present invention, indicating that an amino acid position corresponds to a numerically designated position in SEQ ID NO: 1 thus means that the corresponding position is associated in the alignment as defined above with the numerically designated position in SEQ ID NO: 1 .

[0054] For descriptions of substitutions involving exactly one amino acid position (amino acid substitution), the following convention is used herein: first, the naturally occurring amino acid is designated in the form of an internationally accepted one-letter code, followed by the associated sequence position, and finally the replaced amino acid. Multiple or alternative substitutions within the same polypeptide chain are separated by slashes. Thus, "130D / V" means that position 130 is mutated to D or V. For insertions, the additional amino acids are named according to the sequence position. For deletions, the missing amino acid is replaced by a symbol such as an asterisk or a dash, or Δ is indicated before the corresponding position. For example, P9T describes that the proline at position 9 is substituted by threonine, P9TH describes that the amino acid threonine at position 9 is followed by the insertion of a histidine, and P9* or ΔP9 describes a deletion of the proline at position 9. This nomenclature is known to those skilled in the art of enzyme technology.

[0055] A person skilled in the art can use currently known methods, such as chemical synthesis or polymerase chain reaction (PCR), in combination with standard methods of molecular biology and / or protein chemistry, to produce corresponding nucleic acids or even complete genes based on known DNA and / or amino acid sequences. Such methods are known, for example, from Sambrook, J., Fritsch, E. F. and Maniatis, T. 2001. Molecular cloning: a laboratory manual, 3rd Edition, Cold Spring Laboratory Press.

[0056] In the context of the present invention, cleaning performance is understood to mean the ability of reagent to partially or completely remove existing stains, particularly the whitening performance (lightening performance) on one or more stains on textiles. In addition, in the context of the present invention, cleaning performance also comprises from surface, particularly domestic surface (preferably washing machine or dishwasher, pump tank, storage tank, reservoir, washing drum, rinse chamber, wash basin or sink, toilet urinal, water tank, toilet rim, siphon, water pipe and / or pipeline, water inlet and / or water outlet) remove the ability of microbial-induced sediment, bioaccumulation etc. In the context of the present invention, the reagent comprising the enzyme and the washing / cleaning liquid formed by the reagent and the corresponding enzyme itself have corresponding cleaning performance. Therefore, the cleaning performance of enzyme contributes to the cleaning performance of reagent or the washing / cleaning liquid formed by the reagent. Clean performance is preferably measured in the manner described hereinafter.

[0057] A washing or cleaning liquor is understood to be a working solution containing a washing or cleaning agent which acts on textiles or fabrics or surfaces (in particular dishware or household surfaces) and thus comes into contact with stains present on the textiles or fabrics or surfaces. The washing / cleaning liquor is usually formed at the beginning of the washing / cleaning process and when the agent is diluted with water, for example in a washing machine, dishwasher or other suitable container.

[0058] The cleaning performance on textiles or fabrics can be measured in a washing system containing a detergent and an enzyme to be detected at a dosage between 2.0 and 8.0 grams per liter of washing liquid. The enzyme to be compared is used at the same concentration (based on active protein). The activity of the enzyme is equivalently used to ensure that even if the ratio of active substance to total protein (specific activity value) is slightly different, the respective enzymatic properties, such as the cleaning performance on certain stains, can be compared. Usually, low specific activity can be compensated by adding more protein. In addition, if the enzyme to be detected has different affinities for the test substrate in the activity test, the enzyme to be detected can also be used with the amount or weight of the same substance. "Amount of the same substance" in this context refers to the molar usage of the enzyme to be detected. "Same weight" refers to using the same weight of the enzyme to be detected.

[0059] The enzyme concentration in the washing agent intended for use in this washing system is 0.0001 to 0.1 wt %, in particular 0.001 to 0.1 wt %, more preferably 0.01 to 0.06 wt %, particularly preferably 0.001 to 0.02 wt %, based on active protein.

[0060] Protein concentration can be determined using known methods, such as the BCA method (bicinchoninic acid; 2,2'-biquinoline-4,4'-dicarboxylic acid) or the biuret method (Gornall et al., J. Biol. Chem. 1948, 177: 751-766). In this regard, the active protein concentration can be determined by titrating the active site with a suitable irreversible inhibitor and measuring the residual activity (see Bender et al., J. Am. Chem. Soc. 1966, 88, 24: 5890-5913).

[0061] A liquid reference washing agent for such a washing system may, for example, consist of the following (all figures are in weight percentages (wt %)): 4.4% alkylbenzenesulfonic acid, 5.6% further anionic surfactant, 2.4% C 12-18Fatty acid sodium salt (soap), 4.4% nonionic surfactant, 0.2% phosphonate, 1.4% citric acid, 0.95% NaOH, 0.01% defoamer, 2% glycerol, 0.08% preservative, 1% ethanol, the remainder being deionized water. The dosage of the liquid detergent is preferably between 3.0 and 6.0 grams per liter of washing liquid, for example 3.0, 3.2, 3.5, 3.7, 4.0, 4.5, 4.7, 4.9 or 5.9 grams per liter of washing liquid. The washing process is preferably carried out in a pH range of between pH 7 and pH 10.5, preferably between pH 8 and pH 9.

[0062] The cleaning performance against stains on textiles is determined by measuring the cleanliness of the washed textiles. For example, the washing process can be carried out at 40°C for 60 minutes and the water has a hardness between 15.5°dH and 16.5°dH (German hardness).

[0063] Whiteness, i.e. the whitening of stains, is measured as a measure of cleaning performance using optical measurement methods, preferably photometry. A suitable instrument for this is, for example, a Minolta CM508d spectrometer. Typically, the measurement instrument used is calibrated in advance using a white standard (preferably a supplied white standard).

[0064] The cleaning performance on surfaces, in particular household surfaces (preferably washing machines or dishwashers, pump sump, tanks, reservoirs, washing drums, rinse chambers, washbasins or sinks, toilet bowls, cisterns, toilet rims, siphons, water pipes and / or lines, water inlets and / or water outlets) can be determined as described in Example 1.

[0065] Preferred embodiments of the use and agent according to the invention enable such advantageous cleaning properties even at low temperatures, in particular between 10°C and 60°C, preferably between 15°C and 50°C, more preferably between 20°C and 40°C, and particularly preferably within a temperature range of 30°C.

[0066] In a preferred embodiment, dispersin according to the present invention can be used for washing as described herein and / or cleaning agent.This type of washing according to the present invention and cleaning agent comprise the type of all conceivable washings or cleaning agents, the reagent and concentrate used in undiluted form, it is used for hand-washing or cleaning or for use on a commercial scale in washing machines.These also comprise the cleaning agent such as for textiles, carpets or natural fibers, use the term cleaning agent to this.These also comprise the detergent (automatic dishwasher detergent) or manual dishwashing detergent such as for example for dishwashers, or the cleaning agent for hard surfaces (such as metal, glass, porcelain, pottery, ceramic tile, stone material, painted surface, plastics, timber or leather), use the term cleaning agent to this, promptly except manual and automatic dishwasher detergent, also comprise for example scrubbing agent, glass cleaner, toilet deodorant etc.In context of the present invention, washing and cleaning agent also comprise auxiliary washing agent, it adds in the actual washing agent to realize further effect in manual or automatic textile washing process. Furthermore, in the context of the present invention, washing and cleaning agents also include textile pre- and post-treatment agents, i.e., agents with which the garments come into contact before the actual washing process (e.g., to dissolve stubborn stains), as well as agents that impart further desirable properties to the garments in downstream steps of the actual textile washing process (e.g., a pleasant hand feel, wrinkle resistance, or low static charge). The latter agents include, in particular, softening agents. This also includes agents used in (semi-)automatic washing or cleaning systems (e.g., mopping robots or wet vacuum cleaners).

[0067] The washing and / or cleaning agents according to the present invention can be in the form of a powder or granular solid, in the form of compressed or further compressed particles, in a homogeneous solution or suspension and can contain, in addition to the enzyme according to the present invention, all the conventional ingredients of such agents, preferably at least one further ingredient is present in the agent. In particular, the agents according to the present invention can be surfactants, builders, complexing agents, polymers, glass corrosion inhibitors, corrosion inhibitors, bleaching agents (such as peroxygen compounds), bleach activators or bleach catalysts, water-miscible organic solvents, enzyme stabilizers, sequestering agents, electrolytes, pH regulators and / or other additives (such as optical brighteners, graying inhibitors, dye transfer inhibitors, foam regulators and dyes and perfumes). Advantageous ingredients of the agents according to the present invention are disclosed in the international patent application WO 2009 / 121725, starting from the second to last paragraph on page 5 and ending after the second paragraph on page 13. This disclosure is explicitly cited and the disclosure therein is incorporated into the present patent application.

[0068] The reagent according to the invention advantageously contains the enzyme according to the invention in an amount of 2 μg to 20 mg, preferably 5 μg to 17.5 mg, particularly preferably 20 μg to 15 mg, very particularly preferably 50 μg to 10 mg per gram of reagent.

[0069] The reagents according to the invention are in each case increasingly preferably present in 1×10 -8 % by weight, 0.0001 to 1 wt %, 0.0005 to 0.5 wt %, 0.001 to 0.1 wt %, in each case based on active protein and based on the total weight of the reagent, contain the enzyme according to the invention.

[0070] In a preferred embodiment, the washing and / or cleaning agents according to the invention contain preferably 0.01 to 5% by weight, preferably 0.1 to 4% by weight, particularly preferably 0.15 to 2.5% by weight, of dispersin, based on the total weight of the agent.

[0071] In a preferred embodiment, the washing and / or cleaning agents according to the invention contain at least three further enzymes selected from the group consisting of amylases, proteases, lipases, cellulases, mannanases, pectate lyases and mixtures thereof in a total amount of 0.01 to 10 wt.-%, preferably 0.1 to 8 wt.-%, particularly preferably 0.2 to 6 wt.-%, based on the total weight of the agent.

[0072] In addition, the protease that contains in the reagent and / or other compositions of the reagent can be used at room temperature or when lacking water to the material bag that is impermeable to the enzyme, and this material becomes permeable to the enzyme under the reagent use conditions.Therefore, this embodiment of the present invention is characterised in that said enzyme is at room temperature or when lacking water to the material bag that is impermeable to the enzyme.In addition, the reagent itself can be packaged in a container, preferably in a breathable container, before use shortly or in a washing process, therefrom discharge.

[0073] Other embodiments according to the present invention include all solid, powdered, liquid, gel or pasty dosage forms of reagent according to the present invention, which optionally also can be composed of multiple phases and can exist in compressed or non-compressed form. Reagent can be a free-flowing powder, particularly with a bulk density of 300g / l to 1200g / l, particularly 500g / l to 900g / l or 600g / l to 850g / l. The solid application form of reagent further includes extrudate, granule, tablet or pouch. Alternatively, reagent can also be liquid, gel or pasty form, for example, in non-aqueous liquid washing reagent or non-aqueous paste form, or in aqueous liquid washing reagent or aqueous paste form. Preferred liquid reagents generally. In addition, reagent can be presented with a single-component system. This type of reagent is composed of a phase. Alternatively, reagent also can be composed of multiple phases. This type of reagent is correspondingly divided into multiple components.

[0074] In a preferred embodiment, the agent according to the invention is a textile washing agent.

[0075] In a more preferred embodiment, the agent according to the invention is a liquid textile washing agent.

[0076] In a particularly preferred embodiment, the agent according to the invention is a pre-portioned washing agent, in particular a washing agent portion unit, which comprises a washing agent formulation according to the invention and a water-soluble film which completely encapsulates the washing agent formulation.

[0077] The water-soluble film encapsulating the detergent formulation may comprise one or more structurally different water-soluble polymers. Suitable water-soluble polymers are in particular polymers selected from (optionally acetalized) polyvinyl alcohol (PVAL) and copolymers thereof. The water-soluble film is preferably based on polyvinyl alcohol or a polyvinyl alcohol copolymer with a molecular weight in the range of 10,000 to 1,000,000 g / mol, preferably 20,000 to 500,000 g / mol, particularly preferably 30,000 to 100,000 g / mol, in particular 40,000 to 80,000 g / mol. Suitable water-soluble films are especially sold, for example, under the trade names M8630, M8720, M8310, C8400 or M8900 by the company MonoSol LLC. For example, Aicello Chemical Europe GmbH's products are sold under the trade name PVAL. PT, GA, KC or Films from KL or VF-HP films from Kuraray are also suitable.

[0078] If the washing agents according to the invention are in liquid form, they comprise preferably more than 40% by weight, preferably from 50 to 90% by weight and particularly preferably from 60 to 80% by weight, of water, based on their total weight.

[0079] The agents according to the invention may contain one or more surfactants, of which anionic surfactants, nonionic surfactants and mixtures thereof are particularly suitable, although cationic, zwitterionic and / or amphoteric surfactants may also be present. The agents preferably contain 5 to 70% by weight of surfactant, preferably 35 to 60% by weight, and more preferably 40 to 55% by weight of surfactant.

[0080] Suitable anionic surfactants are in particular soaps and those containing sulfate or sulfonate groups, preferably with alkali metal ions as cations. Useful soaps are preferably saturated or unsaturated C 12-18 Alkali metal salts of fatty acids. Such fatty acids can also be used in an incompletely neutralized form. Suitable sulfate surfactants include C 12-18Salts of sulfuric acid half esters of fatty alcohols and sulfated products of the mentioned nonionic surfactants with a low degree of ethoxylation. Useful sulfonate surfactants include, for example, C 9-14 Alkylbenzene sulfonate, C 12-18 Alkanesulfonates obtained from alkanes (e.g. by chlorosulfonation or sulfoxidation followed by hydrolysis or neutralization), C 12-18 Olefin sulfonates, mixtures of olefin and hydroxyalkane sulfonates, disulfonates (e.g., C 12-18 Monoolefins are obtained by sulfonation with gaseous sulfur trioxide and subsequent alkaline or acid hydrolysis of the sulfonation product) and α-sulfo fatty acid esters (ester sulfonates) obtained by sulfonation of fatty acid methyl or ethyl esters, for example the α-sulfonated methyl esters of hydrogenated coconut, palm kernel or tallow fatty acids.

[0081] The agent preferably contains 2 to 55% by weight, and more preferably 3 to 35% by weight, of anionic surfactants. Very particularly preferably, the agent contains 3 to 25% by weight of alkylbenzenesulfonates. Furthermore, the agent may preferably also contain other anionic surfactants, in particular alkyl ether sulfates, and nonionic surfactants, in particular fatty alcohol alkoxylates. These may constitute the remainder of the surfactant composition.

[0082] Suitable alkylbenzene sulfonates are preferably selected from linear or branched alkylbenzene sulfonates of the following formula:

[0083]

[0084] R′ and R″ are independently hydrogen or alkyl and contain a total of 6 to 19, preferably 7 to 15, in particular 9 to 13 C atoms. A very particularly preferred representative is sodium dodecylbenzenesulfonate.

[0085] C 12-18 Fatty alcohols (e.g., from coconut oil, tallow, lauryl, myristyl, cetyl or stearyl alcohol) or C 10-20 Alkali metal salts (and in particular sodium salts) of sulfuric acid half-esters of oxo alcohols, as well as half-esters of secondary alcohols of these chain lengths are preferred alkyl (alkenyl) sulfates. Also preferred are alkyl (alkenyl) sulfates of the chain lengths mentioned which contain synthetic linear alkyl functional groups, are produced on the basis of petrochemicals and have a degradation behavior similar to that of the appropriate compounds based on fatty chemical raw materials. From a washing point of view, C 12-16 Alkyl sulfate and C 12-15 Alkyl sulfate and C 14-15 Alkyl sulfate.

[0086] Linear or branched C ethoxylated with 1 to 6 moles of ethylene oxide 7-21Sulfate monoesters of alcohols are also suitable, such as 2-methyl branched C 9-11 Alcohol or C with 1 to 4EO 12-18 Fatty alcohol.

[0087] Suitable alkyl ether sulfates are, for example, compounds of the formula:

[0088] R 1 -O-(AO) n -SO3 - X +

[0089] In this formula, R 1 represents a linear or branched, substituted or unsubstituted alkyl functional group, preferably a linear, unsubstituted alkyl functional group, particularly preferably a fatty alcohol functional group. 1 is selected from the group consisting of decyl, undecyl, dodecyl, tridecyl, tetradecyl, pentadecyl, hexadecyl, heptadecyl, octadecyl, nonadecyl, eicosyl functional groups and mixtures thereof, preferably representatives with an even number of C atoms. Particularly preferred functional groups R 1 Derived from C12-18 fatty alcohols such as coconut oil fatty alcohol, tallow fatty alcohol, lauryl alcohol, myristyl alcohol, cetyl alcohol or stearyl alcohol, or C 10-20 Oxo alcohol. AO represents an ethylene oxide (EO) group or a propylene oxide (PO) group, preferably an ethylene oxide group. The subscript n represents an integer from 1 to 50, preferably from 1 to 20, in particular from 2 to 10. Very particularly preferably, n represents the number 2, 3, 4, 5, 6, 7 or 8. X + represents a monovalent cation or one nth of an n-valent cation, in which case alkali metal ions are preferred, including Na + or K + , Na + is the most preferred. Other cations X + Can be selected from NH4 + 、1 / 2Zn 2+ 、1 / 2Mg 2+ 、1 / 2Ca 2+ 、1 / 2Mn 2+ and mixtures thereof.

[0090] In various embodiments, the alkyl ether sulfate may be selected from the group consisting of fatty alcohol ether sulfates of the following formula:

[0091]

[0092] wherein k=11 to 19 and n=2, 3, 4, 5, 6, 7 or 8. Very particularly preferred representatives are Na-C with 2EO (k=11-13, n=2) 12-14Fatty alcohol ether sulfates. The indicated degrees of ethoxylation represent statistical averages and may be integers or fractions for a particular product. The indicated degrees of alkoxylation represent statistical averages and may be integers or fractions for a particular product. Preferred alkoxylates / ethoxylates have a narrow homolog distribution (narrow range ethoxylates, NRE).

[0093] It has been shown that the cold wash performance is advantageous if the agent additionally contains soaps. Therefore, preferred agents are characterized in that they contain soaps. Saturated fatty acid soaps are suitable, for example salts of lauric acid, myristic acid, palmitic acid, stearic acid, hydrogenated erucic acid and behenic acid, in particular soap mixtures derived from natural fatty acids such as coconut fatty acid, palm kernel fatty acid or tallow fatty acid.

[0094] Suitable nonionic surfactants are, in particular, alkyl glycosides and the ethoxylation and / or propoxylation products of linear or branched alcohols or alkyl glycosides, each having 8 to approximately 18 carbon atoms and 3 to 20, preferably 4 to 10, alkyl ether groups in the alkyl moiety. Furthermore, the corresponding ethoxylation and / or propoxylation products of the N-alkylamines, vicinal diols, fatty acid esters and fatty acid amides corresponding to the above-mentioned long-chain alcohol derivatives in the alkyl moiety, as well as the corresponding ethoxylation and / or propoxylation products of alkylphenols having 5 to 12 carbon atoms in the alkyl radical can be used.

[0095] The nonionic surfactants preferably used are alkoxylated, advantageously ethoxylated, in particular primary alcohols having preferably 8 to 18 C atoms and an average of 1 to 12 mol of ethylene oxide (EO) per mol of alcohol, wherein the alcohol functional group may be linear or preferably branched at the 2-position with a methyl group, or may contain a mixture of linear and methyl-branched functional groups, as is customary in oxo alcohol functional groups. However, particular preference is given to alcohol ethoxylates having linear functional groups of naturally derived alcohols having 12 to 18 C atoms (e.g. coconut oil, palm oil, tallow fatty acids or oleyl alcohol) and an average of 2 to 8 EO per mol of alcohol. Preferred ethoxylated alcohols include, for example, C 6-10-11-16 EO with 3 or 4 EO. 12-14 Alcohol, C with 7EO 9-11 Alcohol, C with 3EO, 5EO, 7EO or 8EO 13-15 Alcohol, C with 3EO, 5EO or 7EO 12-18 Alcohols and mixtures thereof, such as C 12-14 Alcohols and C with 5EO 12-18Mixtures of alcohols. The specified degrees of ethoxylation represent statistical averages and may be integers or fractions for specific products. Preferred alcohol ethoxylates have a narrow homolog distribution (narrow range ethoxylates, NRE). In addition to these nonionic surfactants, fatty alcohols with more than 12 EO groups can also be used. Examples of these are tallow fatty alcohols with 14, 25, 30 or 40 EO groups.

[0096] Another class of nonionic surfactants that are preferably used (which can be used alone as nonionic surfactant or in combination with other nonionic surfactants) are alkoxylated, preferably ethoxylated or ethoxylated and propoxylated fatty acid alkyl esters, preferably having 1 to 4 carbon atoms in the alkyl chain, in particular fatty acid methyl esters.

[0097] Another class of nonionic surfactants that can be advantageously used are alkyl polyglycosides (APGs). Useful alkyl polyglycosides satisfy the general formula

[0098] R 0 (G) z , wherein R is a linear or branched (especially a methyl branch at position 2), saturated or unsaturated aliphatic functional group having 8 to 22, preferably 12 to 18, carbon atoms, and G is a symbol representing a glucose unit having 5 or 6 carbon atoms, preferably glucose. The degree of glycosidation z is between 1 and 4, preferably between 1 and 2, and in particular between 1.1 and 1.4. Preference is given to using linear alkyl polyglycosides, i.e., alkyl polyglycosides in which the polyglycoside functional group is a glucose functional group and the alkyl functional group is an n-alkyl functional group.

[0099] Nonionic surfactants of the amine oxide type, such as N-coconut alkyl-N,N-dimethylamine oxide and N-tallow alkyl-N,N-dihydroxyethylamine oxide, and fatty acid alkanolamide nonionic surfactants may also be suitable. The amount of these nonionic surfactants preferably does not exceed the amount of ethoxylated fatty alcohol, in particular does not exceed half of it.

[0100] Suitable amphoteric surfactants are, for example, betaines of the formula:

[0101] (R iii )(R iv )(R v )N + CH2COO -

[0102] where R iii represents an alkyl functional group having 8 to 25, preferably 10 to 21, carbon atoms, optionally interrupted by heteroatoms or heteroatom groups, and R iv and R vrepresents identical or different alkyl functional groups having 1 to 3 carbon atoms, in particular C 10-18 Alkyl dimethyl carboxymethyl betaine and C 11-17 Alkylaminopropyl dimethylcarboxymethyl betaine.

[0103] Suitable cationic surfactants include, in particular, quaternary ammonium compounds of the formula:

[0104] (R vi )(R vii )(R viii )(R ix )N + X -

[0105] where R vi to R ix =X represents four identical or different, in particular two long-chain and two short-chain alkyl functional groups, and X represents an anion, in particular a halide ion, for example didecyldimethylammonium chloride, alkylbenzyldidecylammonium chloride and mixtures thereof. Further suitable cationic surfactants are quaternary surface-active compounds, in particular those with sulfonium, phosphonium, iodonium or arsonium groups, which are also known as antimicrobial active ingredients. By using quaternary surface-active compounds with antimicrobial action, an antimicrobial action can be imparted to the agent, or an antimicrobial action that may already be present due to other ingredients can be improved.

[0106] Complexing agents are another preferred component of the agents of the present invention. Particularly preferred complexing agents are phosphonates, provided their use complies with regulations. In addition to 1-hydroxyethane-1,1-diphosphonic acid, complex phosphonates include a variety of different compounds, such as diethylenetriamine penta(methylenephosphonic acid) (DTPMP). Hydroxyalkane or aminoalkane phosphonates are particularly preferred in this application. Among the hydroxyalkane phosphonates, 1-hydroxyethane-1,1-diphosphonate (HEDP) is particularly important as a cobuilder. It is preferably used in the form of its sodium salt, with the disodium salt reacting neutrally and the tetrasodium salt reacting alkaline (pH 9). Possible aminoalkane phosphonates preferably include ethylenediaminetetramethylenephosphonate (EDTMP), diethylenetriaminepentamethylenephosphonate (DTPMP), and their higher homologues. These are preferably used in the form of neutrally reacting sodium salts, such as the hexasodium salt of EDTMP or the heptasodium and octasodium salts of DTPMP. Among the phosphonates, HEDP is preferably used as a builder. Aminoalkanephosphonates also have a significant heavy metal binding capacity. Therefore, in particular if the agent also contains a bleaching agent, it is preferred to use aminoalkanephosphonates, in particular DTPMP, or mixtures of the aforementioned phosphonates. Preferred agents in the context of the present application contain one or more phosphonates selected from aminotrimethylenephosphonic acid (ATMP) and / or its salts; ethylenediaminetetra(methylenephosphonic acid) (EDTMP) and / or its salts; diethylenetriaminepenta(methylenephosphonic acid) (DTPMP) and / or its salts; 1-hydroxyethane-1,1-diphosphonic acid (HEDP) and / or its salts; 2-phosphonobutane-1,2,4-tricarboxylic acid (PBTC) and / or its salts; hexamethylenediaminetetra(methylenephosphonic acid) (HDTMP) and / or its salts; nitrilotri(methylenephosphonic acid) (NTMP) and / or its salts. Reagents containing 1-hydroxyethane-1,1-diphosphonic acid (HEDP) or diethylenetriaminepenta(methylenephosphonic acid) (DTPMP) as phosphonates are particularly preferred. Of course, the reagents according to the invention may contain two or more different phosphonates.

[0107] The preferred reagents according to the invention are characterized in that the reagent contains at least one complexing agent from the group of phosphonates, preferably 1-hydroxyethane-1,1-diphosphonate, the weight proportion of phosphonate relative to the total weight of the reagent being preferably between 0.1 and 8.0 wt. %, preferably between 0.2 and 5.0 wt. %, more preferably between 0.3 and 3.0 wt. % and particularly preferably between 0.5 and 2.0 wt. %.

[0108] In a further preferred embodiment, the washing and / or cleaning agents according to the invention are essentially free of phosphonate-containing compounds. In this context, "essentially free of phosphonate-containing compounds" means that the respective agent contains less than 2% by weight, preferably less than 1% by weight, more preferably less than 0.5% by weight, and particularly preferably less than 0.1% by weight of phosphonate-containing compounds, based on the total weight of the agent. In a particularly preferred embodiment, these agents are free of phosphonate-containing compounds.

[0109] The agents according to the invention also preferably contain a builder, preferably at least one water-soluble and / or water-insoluble, organic and / or inorganic builder. Builders include in particular silicates, carbonates and / or organic builders.

[0110] As organic builder, polycarboxylate / polycarboxylic acid, polymeric polycarboxylate, aspartic acid, polyacetal, dextrin, other organic builders and phosphonate are especially worthy of attention.These material classes are described below.Can contain this type of organic builder material as required with 40 % by weight at the most, particularly 25 % by weight at the most, and preferably 1 to 8 % by weight amount.Suitable organic builder material is the polycarboxylic acid that for example can use with free acid and / or its sodium salt form, and wherein polycarboxylic acid is interpreted as meaning the carboxylic acid that carries more than an acid-functional group. These are, for example, citric acid, adipic acid, succinic acid, glutaric acid, malic acid, tartaric acid, maleic acid, fumaric acid, glucaric acid and carboxymethylinulin, monomeric and polymeric aminopolycarboxylic acids, in particular glycinediacetic acid, methylglycinediacetic acid, nitrilotriacetic acid (NTA), iminodisuccinic acids (such as ethylenediamine-N,N'-disuccinic acid and hydroxyiminodisuccinic acid), ethylenediaminetetraacetic acid and polyaspartic acid, polyphosphonic acids, in particular aminotris(methylenephosphonic acid), ethylenediaminetetra(methylenephosphonic acid), lysinetetra(methylenephosphonic acid) and 1-hydroxyethane-1,1-diphosphonic acid , polymeric hydroxy compounds (such as dextrins), and polymeric (poly) carboxylic acids, in particular polycarboxylates obtainable by oxidizing polysaccharides or dextrins, and / or polymeric acrylic acid, methacrylic acid, maleic acid and mixed polymers thereof, which may also contain small amounts of polymerizable substances without carboxylic acid functional groups in polymerized form. Such organic builder substances may be contained as needed in an amount of up to 50% by weight, in particular up to 25% by weight, preferably from 10 to 20% by weight, and particularly preferably from 1 to 5% by weight. In addition to their builder effect, the free acids generally also have the property of being acidifying components and are therefore also used to The formulation sets a lower and milder pH. Of particular note here are citric acid, succinic acid, glutaric acid, adipic acid, gluconic acid and any mixtures thereof. Citric acid or a citrate is particularly preferably used as a builder substance. Other particularly preferred builder substances are selected from the group consisting of methylglycine diacetic acid (MGDA), glutamic acid diacetate (GLDA), aspartic acid diacetate (ASDA), hydroxyethyliminodiacetate (HEIDA), iminodisuccinate (IDS), ethylenediamine disuccinate (EDDS), carboxymethyl inulin and polyaspartic acid esters. In preferred In one embodiment, citric acid and / or a citrate is used as a water-soluble organic builder. It is particularly preferred to use 0.5 to 25% by weight, preferably 0.75 to 12.5% ​​by weight, more preferably 1 to 4% by weight of citric acid; and / or 0.5 to 25% by weight, preferably 0.75 to 12.5% ​​by weight, and more preferably 1 to 4% by weight of a citrate, preferably an alkali metal citrate, more preferably sodium citrate. Citric acid / citrate can each be used in the form of a hydrate, for example, citric acid can be used in the form of a monohydrate, and citric acid can be used in the form of trisodium citrate dihydrate.

[0111] Polymeric polycarboxylates are also suitable as wash builders, such as alkali metal salts of polyacrylic acid or polymethacrylic acid, such as those with relative molecular weights of 500 to 70,000 grams per mole. With regard to the present application, the molar mass of the polymeric polycarboxylates referred to is the weight-average molar mass Mw of the corresponding acid form, which has been measured in principle using gel permeation chromatography (GPC), wherein a UV detector has been used. Measurement is carried out for an external polyacrylic acid standard, and due to having a structural dependency with the polymer tested, a true molecular weight value is obtained. These specifications are significantly different from the molecular weight specifications using polystyrene sulfonic acid as a standard. The molar mass recorded for polystyrene sulfonic acid is generally much higher than the molar mass indicated in the present application. Suitable polymers are particularly polyacrylates, which preferably have a molecular weight of 2000 to 20,000 grams per mole. Due to their excellent solubility, molar mass is 2000 to 10,000 grams per mole in this group, and particularly preferably the short-chain polyacrylate of 3000 to 5000 grams per mole may be preferred. Copolymeric polycarboxylates are also suitable, in particular copolymers of acrylic acid and methacrylic acid, and copolymers of acrylic acid or methacrylic acid with maleic acid. Copolymers of acrylic acid and maleic acid containing 50 to 90% by weight of acrylic acid and 50 to 10% by weight of maleic acid have proven particularly suitable. Their relative molecular weight, based on the free acid, is generally 2,000 to 70,000 g / mol, preferably 20,000 to 50,000 g / mol, and in particular 30,000 to 40,000 g / mol.

[0112] The solid dosage form according to the invention preferably contains at least one water-soluble and / or water-insoluble, organic and / or inorganic builder.Water-soluble organic builder substances include the above-mentioned organic builder substances.

[0113] In addition to the above-mentioned water-soluble organic builders, the reagent of the present invention may further contain an inorganic water-soluble builder. Water-soluble inorganic builders include in particular alkali metal silicates, alkali metal carbonates, alkali metal bicarbonates, alkali metal phosphates and / or sesquicarbonates, which may be present in the form of their alkaline, neutral or acidic sodium or potassium salts. Solid fabric washing reagents may optionally also contain a small amount of calcium carbonate. For example, water-soluble crystalline and / or amorphous alkali metal silicates are suitable. The alkali metal silicates that can be used as builders in the reagent according to the present invention preferably have a molar ratio of alkali metal oxide to SiO2 of less than 0.95, in particular 1:1.1 to 1:12, and may exist in amorphous or crystalline form. Preferred alkali metal silicates are sodium silicates, in particular amorphous sodium silicates, having a Na2O:SiO2 molar ratio of 1:2 to 1:2.8. As crystalline silicates (which may exist alone or in admixture with amorphous silicates), preferably used are those having the general formula Na2Si x O 2x+1Crystalline phyllosilicates of the formula yH2O, wherein x (referred to as the modulus) is a number from 1.9 to 22, in particular from 1.9 to 4, and y is a number from 0 to 33, with x preferably being 2, 3, or 4. Preferred crystalline phyllosilicates are those in which x in the general formula mentioned is 2 or 3. In particular, β- and δ-sodium disilicate (Na2Si2O5·yH2O) are preferred. Essentially anhydrous crystalline alkali metal silicates according to the general formula above, wherein x is a number from 1.9 to 2.1 and are produced from amorphous alkali metal silicates, can also be used in the reagents according to the invention. In another embodiment of the reagents according to the invention, crystalline sodium phyllosilicates having a modulus of 2 to 3 are used, which can be prepared from sand and soda. In a further embodiment of the reagent according to the invention, crystalline sodium silicates having a modulus in the range of 1.9 to 3.5 are used. In the reagent containing amorphous and crystalline alkali metal silicates, the weight ratio of amorphous alkali metal silicate to crystalline alkali metal silicate is preferably from 1:2 to 2:1, and in particular from 1:1 to 2:1. The above-mentioned crystalline layered silicates of formula (I) are sold by Clariant GmbH under the trade name Na-SKS, for example Na-SKS-1 (Na2Si 22 O 45 xH2O, nadalite), Na-SKS-2(Na2Si 14 O 29 xH2O, Na-SKS-3(Na2Si8O 17 ·xH2O) or Na-SKS-4 (Na2Si4O9·xH2O, nautilus). Of these, Na-SKS-5 (α-Na2Si2O5), Na-SKS-7 (β-Na2Si2O5, sodium silicate), Na-SKS-9 (NaHSi2O5·3H2O), Na-SKS-10 (NaHSi2O5·3H2O, nautilus), Na-SKS-11 (t-Na2Si2O5) and Na-SKS-13 (NaHSi2O5) are most particularly suitable, but especially Na-SKS-6 (δ-Na2Si2O5). In one embodiment of the reagent according to the invention, a granular compound made of a crystalline layered silicate and a citrate, a crystalline layered silicate and the above-mentioned (co)polycarboxylic acid, or sodium silicate and sodium carbonate is used, for example under the trade name 15 commercially available. Such water-soluble inorganic builder materials are preferably contained in the agents according to the invention in an amount of 1 to 20% by weight, in particular 5 to 15% by weight. Also important water-soluble inorganic builder substances are carbonates (and bicarbonates), in particular sodium carbonate, and phosphonic acid / phosphonates.

[0114] The agents according to the invention are preferably free of phosphate builders, ie they contain less than 1 wt. %, and preferably no phosphate builders are intentionally added.

[0115] The agent may also contain water-insoluble builders. In particular, crystalline or amorphous water-dispersible alkali metal aluminosilicates are used as water-insoluble inorganic builder materials in amounts of up to 50% by weight, preferably not more than 40% by weight, in particular from 3 to 20% by weight, and particularly preferably from 1 to 15% by weight. Of these, preference is given to crystalline sodium aluminosilicates of detergent quality, in particular zeolite A, zeolite P, zeolite MAP and optionally zeolite X, alone or in mixtures, for example cocrystals of zeolite A and X ( Aluminosilicates are preferably prepared in the form of aluminosilicates (e.g., Aluminosilicate AX, available from Condea Augusta SpA). Amounts close to the stated upper limit are preferably used in solid granules. Suitable aluminosilicates particularly have no particles with a particle size greater than 30 μm, and preferably consist of at least 80% by weight of particles with a size less than 10 μm. The calcium binding capacity of these aluminosilicates (determinable according to DE 2412837 A1) is generally in the range of 100 to 200 mg CaO per gram.

[0116] In addition to the above-mentioned builders, the agents may contain cleaning-active polymers. The proportion by weight of cleaning-active polymers relative to the total weight of the agents according to the invention is preferably from 0.1 to 20% by weight, more preferably from 1.0 to 15% by weight and even more preferably from 2.0 to 12% by weight.

[0117] Possible peroxygenates suitable for use in the agents according to the invention include, in particular, organic peroxy acids or perester salts of organic acids, such as salts of phthalimidocaproic acid, peroxybenzoic acid or diperoxydodecanedioic acid; hydrogen peroxide and inorganic salts which release hydrogen peroxide under washing conditions, such salts including perborates, percarbonates, persilicates and / or persulfates such as peroxymonosulfates (caroates), as well as hydrogen peroxide inclusion compounds such as H2O2-urea adducts. Hydrogen peroxide can also be produced by enzyme systems, i.e., oxidases and their substrates. If solid peroxygenates are to be used, they can be used in powder or granular form, which can also be coated in a manner known in principle. The peroxygenates can be added to the washing liquid as such or in the form of agents containing them, which agents can, in principle, contain all conventional washing, cleaning or disinfecting components. Particular preference is given to using alkali metal percarbonates or alkali metal perborate hydrates. If the agents according to the invention contain peroxygenates, these are preferably present in an amount of up to 50% by weight, in particular from 5 to 30% by weight and more preferably from 0.1 to 20% by weight.

[0118] Compounds which, under perhydrolysis conditions, generate aliphatic peroxycarboxylic acids and / or optionally substituted peroxybenzoic acids preferably having 1 to 10 carbon atoms, in particular 2 to 4 carbon atoms, can be used as bleach activators in the reagents. Suitable substances are those which carry O- and / or N-acyl groups and / or optionally substituted benzoyl groups with the specified number of carbon atoms. Preference is given to polyacylated alkylenediamines, in particular tetraacetylethylenediamine (TAED); acylated triazine derivatives, in particular 1,5-diacetyl-2,4-dioxohexahydro-1,3,5-triazine (DADHT); acylated glycolurils, in particular tetraacetyl glycoluril (TAGU); N-acylimides, in particular N-nonanoylsuccinimide (NOSI); acylated phenolsulfonates or carboxylates or their sulfonic or carboxylic acids, in particular nonanoyloxybenzenesulfonate or isononanoyloxybenzenesulfonate or lauroyloxybenzenesulfonate (NOBS or iso-NOBS or LOBS), 4-(2-decanoyloxyethoxycarbonyloxy)benzenesulfonate (DECOBS) or decanoyloxybenzoic acid. Salts (DOBA); carboxylic anhydrides, especially phthalic anhydride; acylated polyols, especially triacetin, ethylene glycol diacetate, 2,5-diacetoxy-2,5-dihydrofuran and enol esters; as well as acetylated sorbitol and mannitol or mixtures thereof (SORMAN); acylated sugar derivatives, especially pentaacetylglucose (PAG), pentaacetylfructose, tetraacetyxylose and octaacetyllactose; acetylated, optionally N-alkylated glucosamine and gluconolactone; N-acylated lactams, such as N-benzoylcaprolactam; nitriles that form perimidic acids, especially aminoacetonitrile derivatives with a quaternized nitrogen atom; and / or oxygen-transferring sulfenyl imides and / or acylhydrazones. Hydrophilically substituted acetals and acyl lactams are also preferably used. Combinations of conventional bleach activators can also be used. Such bleach activators may be present in conventional amounts, preferably in amounts ranging from 0.5 to 10 wt % and especially 1 to 8 wt % based on the total reagent, particularly in the presence of the above-mentioned hydrogen peroxide-generating bleaching agents, but are preferably completely absent when percarboxylic acids are used as the sole bleaching agent.

[0119] In addition to or instead of conventional bleach activators, the solid agent may also contain sulfenyl imines and / or bleach-promoting transition metal salts or transition metal complexes as bleach catalysts.

[0120] Suitable graying inhibitors or soil-releasing active ingredients (soil-releasing polymers) are cellulose ethers such as carboxymethylcellulose, methylcellulose, hydroxyalkylcellulose and mixed cellulose ethers such as methylhydroxyethylcellulose, methylhydroxypropylcellulose and methylcarboxymethylcellulose. Preference is given to using sodium carboxymethylcellulose, hydroxypropylmethylcellulose and mixtures thereof, and optionally mixtures thereof with methylcellulose. Commonly used soil-releasing active ingredients include copolyesters containing dicarboxylic acid units, alkylene glycol units and polyalkylene glycol units. The proportion of graying inhibitors and / or soil-releasing active ingredients in the agents according to the invention is generally not more than 2% by weight, preferably from 0.5 to 1.5% by weight, particularly preferably from 0.5 to 2% by weight.

[0121] For example, derivatives of diaminostilbene disulfonic acid or their alkali metal salts can be contained as optical brighteners, particularly for textiles made of cellulose fibers (e.g., cotton). For example, salts of 4,4'-bis(2-anilino-4-morpholino-1,3,5-triazine-6-amino)stilbene-2,2'-disulfonic acid or compounds with similar structures (wherein the morpholino group is replaced by a diethanolamino group, a methylamino group, or a 2-methoxyethylamino group) are suitable. In addition, substituted 4,4'-distyryldiphenyl groups, such as 4,4'-bis(4-chloro-3-sulfostyryl)diphenyl type brighteners, can be present. A mixture of brighteners can also be used. 1,3-diaryl-2-pyrazoline, such as 1-(p-sulfonamidophenyl)-3-(p-chlorophenyl)-2-pyrazoline type brighteners, and compounds with similar structures are particularly suitable for polyamide fibers. The content of the optical brightener or brightener mixture in the agent is usually not more than 1 wt%, preferably 0.05 to 0.5 wt%.In a preferred embodiment of the present invention, the agent does not contain such active ingredients.

[0122] Conventional foam regulators that can be used in the agents according to the invention include, for example, silicone-silicic acid mixtures, the finely divided silicic acid contained therein preferably being silanized or otherwise hydrophobized. The silicones may comprise both linear compounds and crosslinked silicone resins and mixtures thereof. Further defoamers are paraffins, in particular microparaffins and paraffins having a melting point above 40° C., saturated fatty acids or soaps, in particular having 20 to 22 C atoms, such as sodium behenate, and alkali metal salts of mono- and / or dialkyl phosphates, where the alkyl chains each have 12 to 22 C atoms. Of these, preference is given to using defoamers having C 16-18 Sodium monoalkyl phosphate and / or sodium dialkyl phosphate of alkyl groups. The proportion of foam regulators may be preferably 0.2 to 2% by weight, particularly preferably not more than 1% by weight.

[0123] To set the desired pH value, the reagent according to the invention may contain an acid that is compatible with the system and the environment, in particular citric acid, acetic acid, tartaric acid, malic acid, lactic acid, glycolic acid, succinic acid, glutaric acid, and / or adipic acid, but also mineral acids, in particular sulfuric acid or alkali metal hydrogensulfates, or bases, in particular ammonium or alkali metal hydroxides, preferably sodium hydroxide. Such pH adjusters are preferably present in the reagent according to the invention in an amount of no more than 10 wt %, in particular 0.5 to 6 wt %, and particularly preferably 0.3 to 2 wt %.

[0124] Cleaning agent according to the present invention can contain organic solvent as further component.Adding organic solvent has a favorable effect on the enzyme stability and the cleaning performance of these reagents.Preferred organic solvent is derived from monohydroxy or polyhydroxy alcohol, alkanolamine or glycol ether.Described solvent is preferably selected from ethanol, n-propyl alcohol or isopropyl alcohol, butanol, ethylene glycol, propylene glycol, butylene glycol, glycerine, diethylene glycol, propylene glycol diglycol, butyl diglycol, hexylene glycol, ethylene glycol methyl ether, ethylene glycol ethyl ether, ethylene glycol propyl ether, ethylene glycol mono-n-butyl ether, diethylene glycol methyl ether, diethylene glycol ethyl ether, propylene glycol methyl ether, propylene glycol ethyl ether, propylene glycol propyl ether, dipropylene glycol methyl ether, dipropylene glycol ethyl ether, methoxy triglycol, ethoxy triglycol, butoxy triglycol, 1-butoxy ethoxy-2-propyl alcohol, 3-methyl-3-methoxybutanol, propylene glycol tert-butyl ether and the mixture of these solvents. The weight proportion of these organic solvents relative to the total weight of the reagent according to the invention is preferably 0.1 to 10 wt %, more preferably 0.2 to 8.0 wt %, and even more preferably 0.5 to 5.0 wt %. Particularly preferred organic solvents are glycerol and 1,2-propylene glycol, which are particularly effective in stabilizing the reagent. The liquid reagent contains at least one polyol, preferably selected from glycerol and 1,2-propylene glycol, in an amount of preferably 0.1 to 10 wt %, preferably 0.2 to 8.0 wt %, and more preferably 0.5 to 5.0 wt %, based on the total weight of the reagent. Other preferred organic solvents are organic amines and alkanolamines. The reagent according to the invention contains these amines in an amount of preferably 0.1 to 10 wt %, preferably 0.2 to 8.0 wt %, and more preferably 0.5 to 5.0 wt %, each based on its total weight. Ethanolamine is a particularly preferred alkanolamine.

[0125] The washing and / or cleaning agents according to the invention may contain only the enzymes according to the invention. Alternatively, they may also contain further hydrolases or other enzymes in concentrations that are favorable for the effectiveness of the agents. Therefore, another embodiment of the invention is represented by an agent that further comprises one or more further enzymes. All enzymes that can develop catalytic activity in the agents according to the invention, in particular proteases, lipases, amylases, cellulases, hemicellulases, mannanases, tannases, xylanases, xyloglucanases, β-glucosidases, pectinases, carrageenanases, perhydrolases, oxidases, oxidoreductases and mixtures thereof can preferably be used as further enzymes. The further enzymes are advantageously each present in an amount based on 1×10 40% of the active protein. -8 More and more preferably, the amount of the active ingredient in the agent according to the invention is 1×10 -7 % by weight, 0.00001 to 1 wt %, 0.00005 to 0.5 wt %, 0.0001 to 0.1 wt %, particularly preferably 0.0001 to 0.05 wt %. Particularly preferably, the enzymes exhibit synergistic cleaning performance on specific dirt or stains, i.e. the enzymes contained in the agent composition assist each other in cleaning performance.

[0126] Examples of proteases are subtilisin BPN' from Bacillus amyloliquefaciens and Carlsberg from Bacillus licheniformis, protease PB92, subtilisin 147 and 309, protease from Bacillus lentus, subtilisin DY, and the enzymes thermophilic protease, proteinase K and proteases TW3 and TW7, which are related to the subtilisins in a narrow sense but are no longer similar to the subtilisins. Subtilisin Carlsberg is commercialized in a modified form by Novozymes under the trade name Subtilisin 147 and 309 are available from Novozymes under the trade names and Protease variants derived from the protease from Bacillus lentus DSM 5483 are described, for example, in WO 95 / 23221, WO 92 / 21760, WO 2013 / 060621 and EP 3660151. Other suitable proteases are, for example, those from Novozymes under the trade name Progress Uno and Enzymes available; available under the trade name HYDROGEN® from Danisco / DuPont OxP, Prime, and Preferenz and Preferenz Enzymes available; available from BASF under the trade name Lavergy pro 104 Enzymes available; available under the trade name ANTIBIOL® from Advanced Biochemicals Ltd. Available enzymes; available under the trade name ANTI-PROTEIN® from Wuxi Snyder Bioproducts Ltd. Available enzymes; available under the trade name ANTI-PROPYLENE® from Amano Pharmaceuticals Ltd. and Protease and the enzyme available under the name Proteinase K-16 from KaoCorp. Particular preference is given to using the proteases from Bacillus gibsonii and Bacillus pumilus, which are disclosed in WO 2008 / 086916, WO 2007 / 131656, WO 2017 / 215925, WO 2021 / 175696 and WO 2021 / 175697.

[0127] Examples of amylases are alpha-amylases from Bacillus licheniformis, Bacillus amyloliquefaciens or Bacillus stearothermophilus, and developments thereof which are improved in particular for use in washing and / or cleaning agents. The enzyme from Bacillus licheniformis is marketed by Novozymes under the name Available and marketed by Danisco / DuPont under the name ST is available. The development product of this α-amylase is sold under the trade name and ultra (both from Novozymes), OxAm (Danisco / DuPont) and (DaiwaSeiko Inc.). α-Amylase from Bacillus amyloliquefaciens is available from Novozymes under the name A derivative variant of the α-amylase from Bacillus stearothermophilus is also marketed by Novozymes under the name and For this purpose, the α-amylase from Bacillus sp. A7-7 (DSM 12368) and the cyclodextrin glucanotransferase (CGTase) from Bacillus agaradherens (DSM 9948) should also be emphasized. Fusion products of all the mentioned molecules can also be used. In addition, development products of α-amylases from Aspergillus niger and Aspergillus oryzae (sold by Novozymes under the trade name ANTI-AMYLASE®) are also available. Other commercial products that can be used advantageously are, for example, and or ultra or plus and Amplify TM 12L or Amplify Prime TM 100L (the latter also from Novozymes), and PREFERENZ from Danisco / DuPont series, including for example PREFERENCE PREFERENCE or PREFERENCE Variants of these enzymes obtained by point mutations can also be used according to the invention.

[0128] Suitable cellulases include cellulases of bacterial or fungal origin. Including chemically modified or protein engineered mutants. Suitable cellulases are cellulases from Bacillus, Pseudomonas, Humicola, Fusarium, Thielavia, Acremonium, for example, fungal cellulases from Humicola insolens, Myceliophthorathermophila and Fusarium oxysporum, which are disclosed in US4435307, US5648263, US5691178, US5776757 and WO89 / 09259. Particularly suitable cellulases are alkaline or neutral cellulases with color protection properties. Examples of such cellulases are the cellulases described in EP 0495257, EP 0531372, WO 96 / 11262, WO 96 / 29397 and WO 98 / 08940. Other examples are the cellulase variants described in WO 94 / 07998, EP 0531315, EP 3212777, EP 3502243, EP 3653705, EP 3653706, US 5457046, US 5686593, US 5763254, WO 95 / 24471, WO 98 / 12307, WO 99 / 01544 and WO 2019 / 122520. Examples of cellulases (EC 3.2.1.4) having endo-1,4-glucanase activity are described in WO 2002 / 099091, such as cellulases having a sequence at least 97% identical to the amino acid sequence of positions 1 to 773 of SEQ ID NO: 2 of WO 2002 / 099091. Another example may include a GH44-xyloglucanase, such as a xyloglucanase having a sequence at least 60% identical to positions 40 to 559 of SEQ ID NO: 2 of WO 2001 / 062903. Other examples of cellulases include the GH45 cellulases described in WO 96 / 29397. Commercially available cellulases include Celluzyme TM 、Carezyme TM 、Carezyme Premium TM 、Celluclean TM (e.g. Cellulosean TM 5000L and Celluclean TM 4000T), Celluclean ClassicTM 、Cellusoft TM 、 and Whitezyme TM (Novozymes A / S), Clazinase TM and Puradax HA TM (Genencor International Inc.), KAC-500(B) TM (Kao Corporation)、Revitalenz TM 1000、Revitalenz TM 2000 and Revitalenz TM 3000 (DuPont), and and (AB Enzymes).

[0129] Suitable lipases are, for example, from Thermomyces, for example from T. lanuginosus (formerly Humicola lanuginosa), as described in EP 0 258 068 and EP 0 305 216; from strains of Pseudomonas (some of which are now renamed Burkholderia), for example Pseudomonas alcaligenes or P. pseudoalcaligenes, P. cepacia, Pseudomonas strain SD705, P. wisconsinensis; GDSL-type Streptomyces lipase; from Thermobifida fusca; from Geobacillus stearothermophilus. stearothermophilus); lipase from Bacillus subtilis; and lipases from Streptomyces griseus and S. pristinaespiralis. Preferred lipases include, for example, those originally obtainable from or developed by Humicola lanuginosa (Thermomyces lanuginosus), in particular from the lipases mentioned, having one or more of the following amino acid substitutions in positions D96L, T213R and / or N233R, with T213R and N233R being particularly preferred. Preferred commercial lipase products include Lipolase TM 、Lipex TM 、Lipolex TM and Lipoclean TM (Novozymes A / S), Lumafast (Genencor / DuPont) and Lipomax (Gist-Brocades).

[0130] Suitable mannanases are, for example, Bacillus subtilis endo-β-mannanase, Bacillus I633 endo-β-mannanase, Bacillus AAI12 endo-β-mannanase, Bacillus AA349 endo-β-mannanase, Bacillus adhering NCIMB 40482 endo-β-mannanase, Bacillushalodurans endo-β-mannanase, Bacillus clausii endo-β-mannanase, Bacillus licheniformis endo-β-mannanase, Humicola insolens endo-β-mannanase and Caldocellulosiruptor sp. endo-β-mannanase (e.g., US 6060299, WO 99 / 11268). 99 / 64573, US6566114 and WO 99 / 64619).

[0131] Pectate lyases suitable for washing and / or cleaning agents are described, for example, in WO 2003 / 095638 or WO 2015 / 121133. Examples of suitable pectinolytic enzymes are also available from Novozymes under the trade name Pektinex or Available from AB Enzymes under the trade name Rohapect Rohapect Rohapect Rohapect Rohapect MAplus HC, Rohapect Rohapect Rohapect Available from Diversa Corp under the trade name Available enzymes and enzyme preparations.

[0132] In the cleaning agents described herein, the enzymes to be used may further be formulated with accompanying substances (eg from fermentation).In liquid formulations, the enzymes are preferably used as liquid enzyme formulations.

[0133] The enzymes are usually not provided in the form of pure proteins, but in the form of stable, storable and transportable preparations. These ready-to-use preparations include solid preparations obtained, for example, by granulation, extrusion or freeze-drying, or, in particular in the case of liquids or gels, enzyme solutions which are advantageously as concentrated as possible, have a low water content and / or are admixed with stabilizers or other builders.

[0134] Alternatively, the enzymes can also be encapsulated, either in solid or liquid dosage form (e.g. by spray drying or extrusion of enzyme solutions together with preferably natural polymers) or in the form of capsules (e.g. capsules in which the enzyme is encapsulated in a solidified gel or core-shell capsules in which the enzyme-containing core is encapsulated by a protective layer impermeable to water, air and / or chemicals). Further active ingredients such as stabilizers, emulsifiers, pigments, bleaching agents or dyes can additionally be applied in superimposed layers. Such capsules are manufactured using methods known per se, for example by vibratory granulation or roller granulation, or by a fluidized bed process. Advantageously, such granules are less dusty due to the application of polymeric film-forming agents and are storage stable due to the coating.

[0135] Furthermore, it is possible to formulate two or more enzymes together so that a single particle exhibits multiple enzyme activities.

[0136] The enzyme can also be introduced into a water-soluble film, such as those used in the formulations of washing and cleaning agents in unit dosage form. Such films allow the enzyme to be released upon contact with water. As used herein, "water-soluble" refers to a film structure that is preferably completely water-soluble. Preferably, such films are composed of (completely or partially hydrolyzed) polyvinyl alcohol (PVA).

[0137] The agent according to the present invention may contain one or more reversible enzyme inhibitors / stabilizers. The agent according to the present invention may contain a reversible enzyme inhibitor / stabilizer in a concentration of 0.1 to 2 wt %, preferably 0.3 to 1.5 wt %, based on the total weight of the agent. If several inhibitors / stabilizers are included, this information refers to the total concentration.

[0138] These may in particular be chosen from the group of polyols (such as glycerol or 1,2-ethylene glycol), benzamidine hydrochloride, borax, boric acid, boronic acid or salts or esters or derivatives thereof, in particular phenylboronic acid derivatives or 4-formylphenylboronic acid (4-FPBA), antioxidants, special peptide compounds and combinations thereof.

[0139] In the context of the present invention, "phenylboronic acid derivatives" are understood to mean compounds of formula (III). The compounds of formula (III) have the following structural formula:

[0140]

[0141] Where R is hydrogen, hydroxyl group, C 1-6 Alkyl groups, substituted C 1-6 Alkyl group, C 1-6 Preferably, the functional group R in the phenylboronic acid derivative is C 1-6Alkyl groups, and among these, -CH3, -CH3CH2 or -CH3CH2CH2 is more preferred. More preferably, the functional group R in the phenylboronic acid derivative is hydrogen. A particularly preferred phenylboronic acid derivative is 4-formylphenylboronic acid (4-FPBA).

[0142] The inhibitor / stabilizer compound used may be boric acid.

[0143] In a particularly preferred embodiment, the washing and / or cleaning agents according to the invention contain essentially no boron-containing compounds. "Essentially free of boron-containing compounds" in this context means that the agents according to the invention contain less than 2% by weight, preferably less than 1% by weight, more preferably less than 0.5% by weight, and particularly preferably less than 0.1% by weight of boron-containing compounds, based on the total weight of the agent. In a very particularly preferred embodiment, the agents according to the invention contain no boron-containing compounds, i.e., in particular, they do not contain any boric acid and / or phenylboronic acid derivatives.

[0144] In various embodiments, the enzyme and inhibitor / stabilizer compound can be preformulated in an enzyme composition. As is apparent from the preceding statements, the enzyme protein only constitutes a portion of the total weight of conventional enzyme preparations. Preferably, the enzyme preparation used contains 0.1 to 40 wt %, preferably 0.2 to 30 wt %, particularly preferably 0.4 to 20 wt %, and in particular 0.8 to 10 wt % of enzyme protein. In such compositions, the inhibitor / stabilizer compound can be included in an amount of 0.05 to 35 wt %, preferably 0.05 to 10 wt %, based on the total weight of the enzyme composition. This enzyme composition (also a component of the present invention) can then be used in the reagent according to the invention, in particular in an amount that results in the final concentration indicated above being reached in the reagent.

[0145] Another object of the present invention is an agent for cleaning surfaces, in particular household surfaces, preferably in washing machines or dishwashers, pump sumps, tanks, cisterns, washing drums, rinse chambers, wash basins or sinks, toilets, water tanks, toilet rims, siphons, water pipes and / or lines, water inlets and / or water outlets, in particular for preventing and / or reducing odors and / or for preventing and / or reducing and / or removing microbiologically induced deposits, and / or for improving hygiene, characterized in that the agent contains dispersin, wherein the dispersin has a β-1,6-N-acetylglucosaminidase activity and comprises an amino acid sequence that is at least 70% (and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99%) identical to the amino acid sequence given in SEQ ID NO: 1 over its entire length, and based on the method according to SEQ ID NO: NO:1 number, in the corresponding position 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71, 72, 74, 77, 79, 80, 81, 82, 90, 99, 100, 103, 104, 105, 106, 107, 110, 1 11, 113, 114, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 127, 128, 131, 135, 138, 139, 140, 142, 145, 147, 148, 149, 150, 151, 152, 163, 164, 167, 168, 170, 171, 173, 174, 175, 177, 178, 179, 180 1, 185, 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221, 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251, 252, 253, 254, 256, 260, 261, 262, 263, 264, 265 , 267, 268, 270, 271, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321 and 323. This also includes agents for (semi-)automatic washing or cleaning systems (such as mopping robots or wet vacuum cleaners).

[0146] A preferred embodiment relates to an agent for cleaning surfaces, in particular household surfaces, preferably washing machines or dishwashers, pump sumps, tanks, reservoirs, washing drums, rinse chambers, wash basins or sinks, toilets, water tanks, toilet rims, siphons, water pipes and / or pipelines, water inlets and / or water outlets, in particular for preventing and / or reducing odors and / or for preventing and / or reducing and / or removing microbially induced deposits, and / or for improving hygiene, characterized in that the agent contains dispersin, wherein the dispersin has β-1,6-N-acetylglucosaminidase activity and comprises the residue corresponding to SEQ ID 1 is at least 70% (and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99%) identical to the amino acid sequence given in NO: 1 over its entire length and is based on the amino acid sequence according to SEQ ID NO:1 number, in the corresponding position 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71, 72, 74, 77, 79, 80, 81, 82, 90, 99, 100, 103, 104, 105, 106, 1 07, 110, 111, 113, 114, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 127, 128, 131, 135, 138, 139, 140, 142, 145, 147, 148, 149, 150, 151, 152, 163, 164, 167, 168, 170, 171, 173, 174, 175 5, 177, 178, 179, 181, 185, 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221, 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251, 252, 253, 254, 256 、260、261、262、263、264、265、267、268、270、271、272、273、274、276、278、279、280、281、282、283、284、287、288、290、291、296、300、301、303、304、305、306、308、309、312、314、315、319、, S23E, S23I, S23L, S23R, S23T, S23V, V25R, D26M, Y30, Y30D, Y31E, Y32A, Y33D, Y34E, Y35I, Y36H, Y37H, Y38L, Y39D, Y40H, Y41E, Y42H, Y43D, Y44H, Y45E, Y46H, Y47H, Y48L, Y49D, Y49N, Y41E, Y49 Y30L, Y30M, Y30N, Y30R, Y30T, Y30V, G32L, G32M, G32R, N43*, N43H, N43L, E4 4*, N45D, N45L, N45V, A49W, A49Y, Y52*, Y52M, G54L, G54M, G54N, S56T, S56W , S57W, E58N, N59A, N59C, N59D, N59E, N59F, N59M, N59R, N59V, N59W, T60V, N 62C, N62D, N62H, N62Q, N62W, T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67L , N68L, N68Q, L71H, L71N, L71R, L71V, L71W, S72*, S72C, S72D, S72E, S72F, S 72G, S72I, S72M, S72N, S72R, S72T, S72Y, I74L, S77A, D79V, K80*, K80E, K80 H, K80L, K80N, K80Q, K80V, K80W, D81A, D81G, D81L, D81R, D81S, D81T, D81V, D81W, I82V, L90F, E99Q, E99R, L100S, K103A, K103R, K104N, K104W, D105N, V1 06A, V106D, V106E, V106H, V106K, V106L, V106M, V106N, V106Q, V106W, V106 Y, K107A, K107C, K107L, K107M, K107T, K107V, K107W, N110M, N110R, N110V, D 111A, D111E, D111M, D111N, D111Q, D111R, D111V, D111W, V113T, T114C, T11 4S, Y116D, Y116N, Y116R, S117*, S117D, S117H, S117N, S117P, E118*, E118A,E118D、E118G、E118L、E119G、E119W、T120I、T120L、T120M、T120V、T120W、D122*、D122H、D122R、Y123W、Y124C、Y124I、Y124K、Y124L、Y124M、Y124Q、Y124R、Y124T、Y124V、Y124W、D125C、D125G、D125K、D125Q、D125R、N126V、R127D、R127H、R127K、R127L、R127M、R127Q、R127W、V128C、V128L、V128T、D131V、Q135*、Q135A、Q135D、Q135E、Q135K、Q135M、Q135Y、D138K、D138L、D138M、D138Q、D138R、D138S、D138V、D138W、E139W、D142R、D142W、Y145*、Y145H、Y145L、Y145N、Y145V、P147A、P147C、P147D、P147F、P147G、P147L、P147M、P147R、P147S、P147T、P147V、K148A、K148D、K148L、K148V、F149L、F149M、F149N、E150D、E150H、E150K、E150L、E150M、E150N、E150R、E150V、E150W、E150Y、G151A、G151C、G151D、G151L、G151N、G151P、G151S、G151W、K152D、K152L、K152R、G164D、G164E、G164H、G164S、G164V、V167D、V167E、V167L、V167P、V167Q、V167R、V167W、H168N、L170A、L170D、L170E、L170F、L170H、L170K、L170M、L170N、L170P、L170Q、L170R、L170S、L170V、L170W、L170Y、D171A、D171C、D171E、D171K、D171L、D171M、D171Q、D171R、D171V、D171W、D171Y、I173C、D174H、D174M、D174N、D174V、D174W、F175Y、N177M、Q178*、Q178A、Q178K、Q178R、Q178W、I179T、S181C、S181D、S181F、S181G、S181N、S181P、S181Q、S181T、S181V、S181W、E185M、E185R、E185V、E185W、S186D、S186E、S186H、S186I、S186K、S186L、S186M、S186N、S186Q、S186R、S186V、S186W、K187C、K187D、K187G、K187R、K187S、K187V、K187W、Y188P、E189L、E189V、E189W、S199C、S199L、S199M、S199Y、E200D、E200F、E200K、E200L、E200M、E200N、E200R、E200W、A203C、A203D、A203E、A203G、A203L、A203M、A203P、A203R、A203S、A203T、A203V、A203W、N204L、N204M、N204V、N204W、N204Y、L205I、D207A、D207C、D207E、D207G、D207N、D207Q、D207S、D207V、D207W、S208A、S208C、S208D、S208G、S208L、S208Q、S208T、S208V、S208W、S210T、Q215R、Q215M、Q215L、Q215*、S217V、T218A、T218L、T218Q、T218R、T218V、G222D、E224A、E224P、N227A、N227Q、N227R、N227S、N227T、N227K、D230*、D230R、D230T、D230W、E232D、E232V、N233H、N233Q、N233R、N233W、W234R、G235W、G235A、G235E、G235F、G235H、G235I、G235L、G235M、G235N、G235P、G235S、G235V、S237C、S237G、S237M、S237N、S237W、S237Y、Y244C、Y244E、Y244M、L249H、L249K、L249Q、L249R、L249W、L249Y、S251L、S251N、S251R、S251W、N252P、N252C、G253D、G253W、F254I、F254L、F254M、F254Y、Q256E、Q256R、N260*、N260A、N260C、N260E、N260I、N260K、N260L、N260M、N260Q、N260R、N260V、N260W、N260Y、E261*、E261A、E261D、E261R、E261W、Q262*、Q262F, Q262H, Q262W, Q262Y, M263K, M263L, M263Q, D264*, D264C, D264E, Y265F, N267S, N267T, W268C, W268E, W268M, W2 68R, Y270F, A271D, A271G, H272D, H272I, Y281P, Y282E, Y282N, Y281R, H272W, N273W, K274R, K274A, K274H, F276A, F276C , F276K, F276N, F276G, F276L, F276M, F276P, F276S, F276V, F276W, I278A, I278K, I278N, I278Q, I278V, S279C, S279D, S2 79E, S279G, S279N, D280C, D280E, Y281*, Y281A, Y281C, Y281H, Y281K, Y281N, Y281P, Y282E, Y282N, H283I, A284I, A284L , A284N, A284P, A284T, A284V, T287N, S288D, S288K, S288N, V290I, K291L, K291R, K291V, T296C, E300A, E300D, H301C, H 301N, H301R, T303A, T303C, T303G, T303K, T303Q, T303R, T303W, D304C, D304M, L305M, L305N, S306C, K308A, K308D, K308 G, K308I, K308L, K308Q, K308S, K308T, K308V, K308Y, K309A, K309C, K309D, K309H, K309L, K309M, K309N, K309Q, K309S, K309T, K309I, K312A, K312L, K312M, K312N, K312Q, K312S, K312W, E314I, E314L, E314V, L315I, L315V, R319A, N323R and combinations thereof. This also includes agents for (semi-)automatic washing or cleaning systems, such as mopping robots or wet vacuum cleaners.

[0147] Another object of the present invention relates to a method for cleaning surfaces, in particular household surfaces, preferably in washing machines or dishwashers, pump sumps, storage tanks, water reservoirs, washing drums, rinse chambers, washbasins or sinks, toilets, water tanks, toilet rims, siphons, water pipes and / or pipelines, water inlets and / or water outlets, in particular for preventing and / or reducing odors and / or for preventing and / or reducing and / or removing microbiologically induced deposits and / or for improving hygiene, characterized in that a dispersant according to the invention as described herein and / or an agent according to the invention as described herein is used in at least one method step, the method preferably being carried out in a temperature range of about 10°C to about 60°C, preferably about 15°C to about 40°C, particularly preferably about 20°C to about 30°C.

[0148] This includes both manual and machine methods, with machine methods being preferred because they allow for more precise control, for example regarding the amount used and the contact time. Textile cleaning methods are generally characterized by the fact that, in a number of process steps, a plurality of cleaning active substances are applied to the material to be cleaned and rinsed off after an exposure period, or the material to be cleaned is treated in some other way with a detergent or a solution or dilution of such an agent. This also includes methods using (semi-)automatic washing or cleaning systems, such as mopping robots or wet vacuum cleaners.

[0149] This object of the present invention also includes a dishwasher method. The detergent according to the invention can be dispensed into the cleaning liquid in a method, for example, via a dispensing chamber in the door or via an additional dispensing container inside the dishwasher. Alternatively, the detergent can be applied directly to the dirty dishes or to an inner wall of the dishwasher, such as the inside of the door. The method according to the invention is carried out inside a commercially available dishwasher. In the case of dishwashers, the user can usually select and determine the cleaning program before carrying out the dishwashing method. The dishwasher cleaning program used in the method according to the invention includes at least one prewash cycle and one cleaning cycle. According to the invention, a cleaning program preferably includes a further cleaning or rinsing cycle (e.g., a rinse cycle). The method according to the invention is particularly preferably part of a cleaning program that includes a prewash cycle, a cleaning cycle, and a rinse cycle. The method according to the invention is preferably used for a cleaning program in which the washing liquid is heated during the cleaning cycle. In a preferred embodiment of the method according to the invention, the cleaning cycle in which the detergent according to the invention is dispensed into the dishwasher is characterized in that the temperature of the cleaning liquid rises to a value above 30°C, preferably above 40°C, and in particular above 50°C during the cycle.

[0150] An alternative embodiment of this subject of the present invention is also represented by a method for treating textile raw materials or for textile care, in which the agent according to the invention acts in at least one method step. Of these, preference is given to methods for textile raw materials, fibers or textiles containing natural components, and very particularly for those containing wool or silk.

[0151] All aspects, objects and embodiments described for the agents according to the invention also apply to these subjects of the invention. Therefore, explicit reference is made to the disclosure of the corresponding points at this point and it is pointed out that this disclosure also applies to the above-mentioned use according to the invention. Example

[0152] Example 1: Determination of odor and biodeposit formation

[0153] Table 1: Washing reagent matrices used

[0154]

[0155] Before the test began, the household washing machine used (Miele Novotronic W1514) was subjected to a sensory and visual assessment of the formation of deposits / biodeposits on the washing machine pump sump (- = no biodeposits, + = mild microbial deposits, ++ = strong biodeposits). Furthermore, for preparation, approximately 2.0 kg of white textiles (T-shirts, terry cloth) were cleaned and disinfected using a commercially available heavy-duty detergent containing bleach by repeated washing (using the intensive program (95°C, 16° dH, 1400 rpm, Miele Novotronic W1514)) and dried in a tumble dryer. The prepared laundry was repeatedly washed in a prepared household washing machine (30°C, 16° dH, 1400 rpm for 120 minutes) and stored under standardized conditions, making it possible to simulate typical household washing conditions and thus induce the corresponding deposit / biodeposits in the washing machine. Between wash cycles, the laundry was stored outside the washing machine with the door closed for incubation. After the last wash cycle, a sensory evaluation of the washing machine (i.e., with the washing drum open) was performed by a mixed expert / consumer panel (odor scale 0-3, 0 = no odor, 3 = strong odor; difference ≥ 0.5 = significant). In addition to the sensory evaluation, a visual assessment of the sediment / biological deposit formation on the washing machine pump sump was performed (- = no biological deposits, + = mild microbiological deposits, ++ = severe biological deposits).

[0156] Table 2: Odors in washing machines

[0157]

[0158]

[0159] *Dispersin according to the present invention: SEQ ID NO: 1+Q3I-H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-G235W-N252P-N260Q-H272V-S279D-Y281P-K308Q-K309E-K312Q

[0160] Table 3: Biodeposits in the pump sump

[0161] Before the first wash After the experiment is completed Formulation A ++ ++ Formulation A + Dispersin* ++ - Formulation B ++ ++ Formulation B + Dispersin* ++ - Formulation C ++ ++ Formulation C + Dispersin* ++ - Formulation D ++ ++ Formulation D + Dispersin* ++ -

[0162] *Dispersin according to the present invention: SEQ ID NO: 1+Q3I-H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-G235W-N252P-N260Q-H272V-S279D-Y281P-K308Q-K309E-K312Q

[0163] The results show that dispersin according to the invention not only reduces or prevents the formation of odors and biodeposits in washing machines, it even removes already existing biodeposits.

[0164] Example 2: Example Formulations

[0165] The dispersins according to the invention can be used in a variety of washing and cleaning agents and achieve their effects.

[0166] Table 4: Liquid Washing Reagents

[0167]

[0168] Table 5: Liquid Washing Reagents

[0169]

[0170] Table 6: Solid washing reagents

[0171]

[0172]

[0173] Table 7: Hand Dishwashing Detergents

[0174] Chemical name Wt% of active substance in the formulation Deionized water margin FAEOS 8.8 Cocamidopropyl Betaine 1.2 calcium lactate 1.0 Amylase (Stainzyme 12L) 0.8 essence 0.2 dye 0.01 Salt 2.0 Dispersin 0.1 pH 8.0

[0175] Table 8: Two-Phase Dishwashing Detergents

[0176]

[0177]

Claims

1. Dispersin is used for the following purposes: Prevention and / or reduction of odors in surfaces, in particular domestic surfaces, preferably washing machines or dishwashers, pump sump, tanks, reservoirs, washing drums, rinse chambers, wash basins or sinks, toilets, cisterns, toilet rims, siphons, pipes and / or lines, water inlets and / or water outlets, and / or Prevention and / or reduction and / or removal of microbiologically induced deposits in surfaces, in particular domestic surfaces, preferably washing machines or dishwashers, pump sump, tanks, cisterns, washing drums, rinse chambers, wash basins or sinks, toilets, cisterns, toilet rims, siphons, water pipes and / or lines, water inlets and / or water outlets, and / or Improving hygiene in surfaces, in particular domestic surfaces, preferably in washing machines or dishwashers, pump sump, tanks, cisterns, washing drums, rinse chambers, washbasins or sinks, toilets, water tanks, toilet rims, siphons, water pipes and / or lines, water inlets and / or water outlets; wherein the dispersin has β-1,6-N-acetylglucosaminidase activity and comprises an amino acid sequence that is at least 70%, and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identical to the amino acid sequence given in SEQ ID NO: 1 over its entire length, and based on the method according to SEQ ID NO: 90, 99, 100, 103, 104, 105, 106, 107, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145 111, 113, 114, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 127, 128, 131, 135, 138, 139, 140, 142, 145, 147, 148, 149, 150, 151, 152, 163, 164, 167, 168, 170, 171, 173, 174, 175, 177, 178, 179, 180 81, 185, 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221, 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251, 252, 253, 254, 256, 260, 261, 262, 263, 264, 265 5, 267, 268, 270, 271, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321 and 323 have at least one amino acid substitution and / or deletion.

2. The method of claim 1 , wherein the at least one amino acid substitution and / or deletion is selected from the group consisting of: D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19N, E19P, K22A, K22M, K22V, S23C, S23E, S23I, S23L, S23R, S23T, S23V, V25R, D26M, Y30*, Y30D, Y30L, Y30M, Y30N, Y30R , Y30T, Y30V, G32L, G32M, G32R, N43*, N43H, N43L, E44*, N45D, N45L, N45V, A 49W, A49Y, Y52*, Y52M, G54L, G54M, G54N, S56T, S56W, S57W, E58N, N59A, N59 C, N59D, N59E, N59F, N59M, N59R, N59V, N59W, T60V, N62C, N62D, N62H, N62Q, N62W, T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71H, L71 N, L71R, L71V, L71W, S72*, S72C, S72D, S72E, S72F, S72G, S72I, S72M, S72N, S72R, S72T, S72Y, I74L, S77A, D79V, K80*, K80E, K80H, K80L, K80N, K80Q, K8 0V, K80W, D81A, D81G, D81L, D81R, D81S, D81T, D81V, D81W, I82V, L90F, E99Q , E99R, L100S, K103A, K103R, K104N, K104W, D105N, V106A, V106D, V106E, V10 6H, V106K, V106L, V106M, V106N, V106Q, V106W, V106Y, K107A, K107C, K107L , K107M, K107T, K107V, K107W, N110M, N110R, N110V, D111A, D111E, D111M, D 111N, D111Q, D111R, D111V, D111W, V113T, T114C, T114S, Y116D, Y116N, Y11 6R, S117*, S117D, S117H, S117N, S117P, E118*, E118A, E118D, E118G, E118L,E119G、E119W、T120I、T120L、T120M、T120V、T120W、D122*、D122H、D122R、Y123W、Y124C、Y124I、Y124K、Y124L、Y124M、Y124Q、Y124R、Y124T、Y124V、Y124W、D125C、D125G、D125K、D125Q、D125R、N126V、R127D、R127H、R127K、R127L、R127M、R127Q、R127W、V128C、V128L、V128T、D131V、Q135*、Q135A、Q135D、Q135E、Q135K、Q135M、Q135Y、D138K、D138L、D138M、D138Q、D138R、D138S、D138V、D138W、E139W、D142R、D142W、Y145*、Y145H、Y145L、Y145N、Y145V、P147A、P147C、P147D、P147F、P147G、P147L、P147M、P147R、P147S、P147T、P147V、K148A、K148D、K148L、K148V、F149L、F149M、F149N、E150D、E150H、E150K、E150L、E150M、E150N、E150R、E150V、E150W、E150Y、G151A、G151C、G151D、G151L、G151N、G151P、G151S、G151W、K152D、K152L、K152R、G164D、G164E、G164H、G164S、G164V、V167D、V167E、V167L、V167P、V167Q、V167R、V167W、H168N、L170A、L170D、L170E、L170F、L170H、L170K、L170M、L170N、L170P、L170Q、L170R、L170S、L170V、L170W、L170Y、D171A、D171C、D171E、D171K、D171L、D171M、D171Q、D171R、D171V、D171W、D171Y、I173C、D174H、D174M、D174N、D174V、D174W、F175Y、N177M、Q178*、Q178A、Q178K、Q178R、Q178W、I179T、S181C、S181D、S181F、S181G、S181N、S181P、S181Q、S181T、S181V、S181W、E185M、E185R、E185V、E185W、S186D、S186E、S186H、S186I、S186K、S186L、S186M、S186N、S186Q、S186R、S186V、S186W、K187C、K187D、K187G、K187R、K187S、K187V、K187W、Y188P、E189L、E189V、E189W、S199C、S199L、S199M、S199Y、E200D、E200F、E200K、E200L、E200M、E200N、E200R、E200W、A203C、A203D、A203E、A203G、A203L、A203M、A203P、A203R、A203S、A203T、A203V、A203W、N204L、N204M、N204V、N204W、N204Y、L205I、D207A、D207C、D207E、D207G、D207N、D207Q、D207S、D207V、D207W、S208A、S208C、S208D、S208G、S208L、S208Q、S208T、S208V、S208W、S210T、Q215R、Q215M、Q215L、Q215*、S217V、T218A、T218L、T218Q、T218R、T218V、G222D、E224A、E224P、N227A、N227Q、N227R、N227S、N227T、N227K、D230*、D230R、D230T、D230W、E232D、E232V、N233H、N233Q、N233R、N233W、W234R、G235W、G235A、G235E、G235F、G235H、G235I、G235L、G235M、G235N、G235P、G235S、G235V、S237C、S237G、S237M、S237N、S237W、S237Y、Y244C、Y244E、Y244M、L249H、L249K、L249Q、L249R、L249W、L249Y、S251L、S251N、S251R、S251W、N252P、N252C、G253D、G253W、F254I、F254L、F254M、F254Y、Q256E、Q256R、N260*、N260A、N260C、N260E、N260I、N260K、N260L、N260M、N260Q、N260R、N260V、N260W、N260Y、E261*、E261A、E261D、E261R、E261W、Q262*、Q262F、Q262H、Q262W、Q262Y, M263K, M263L, M263Q, D264*, D264C, D264E, Y265F, N267S, N267T, W268C, W268E, W268M, W268R, Y270F, A271D, A271G, H272D, H272I, Y281P, Y282E, Y282N, Y281R, H272W, N273W, K274R, K274A, K274H, F276A, F276C, F276K, F276N, F 276G, F276L, F276M, F276P, F276S, F276V, F276W, I278A, I278K, I278N, I278Q, I278V, S279C, S279D, S279E, S279G, S 279N, D280C, D280E, Y281*, Y281A, Y281C, Y281H, Y281K, Y281N, Y281P, Y282E, Y282N, H283I, A284I, A284L, A284N, A2 84P, A284T, A284V, T287N, S288D, S288K, S288N, V290I, K291L, K291R, K291V, T296C, E300A, E300D, H301C, H301N, H3 01R, T303A, T303C, T303G, T303K, T303Q, T303R, T303W, D304C, D304M, L305M, L305N, S306C, K308A, K308D, K308G, K30 8I, K308L, K308Q, K308S, K308T, K308V, K308Y, K309A, K309C, K309D, K309H, K309L, K309M, K309N, K309Q, K309S, K309T, K309I, K312A, K312L, K312M, K312N, K312Q, K312S, K312W, E314I, E314L, E314V, L315I, L315V, R319A, N323R, and combinations thereof.

3. Use of a dispersant according to claim 1 or 2 for preventing and / or reducing odors in surfaces, in particular household surfaces, preferably washing machines or dishwashers, pump sump, storage tanks, cisterns, washing drums, rinse chambers, washbasins or sinks, toilets, water tanks, toilet rims, siphons, water pipes and / or pipelines, water inlets and / or water outlets.

4. Use of a dispersin according to claim 1 or 2 for preventing and / or reducing and / or removing microbiologically induced deposits on surfaces, in particular household surfaces, preferably washing machines or dishwashers, pump sump, storage tanks, reservoirs, washing drums, rinse chambers, washbasins or sinks, toilets, water tanks, toilet rims, siphons, water pipes and / or pipelines, water inlets and / or water outlets.

5. Use of a dispersant according to claim 1 or 2 for improving the hygiene of surfaces, in particular domestic surfaces, preferably in washing machines or dishwashers, pump sump, storage tanks, water reservoirs, washing drums, rinse chambers, washbasins or sinks, toilets, water tanks, toilet rims, siphons, water pipes and / or pipelines, water inlets and / or water outlets.

6. The method according to claim 1 , wherein the dispersin is contained in a washing and / or cleaning agent, wherein the agent is substantially free of boron-containing compounds, preferably free of boron-containing compounds; and / or the pH of a 1 wt % solution of the agent in deionized water at 20° C. is in the range of from about 6 to about 11, in particular from about 6.5 to about 10.5, more preferably from about 7 to about 10, particularly preferably from about 8 to about 9.

7. The method according to claim 1, wherein the temperature is preferably in the range of about 10°C to about 60°C, preferably about 15°C to about 40°C, particularly preferably 20°C to about 30°C.

8. An agent for cleaning surfaces, in particular household surfaces, preferably washing machines or dishwashers, pump sump, tanks, reservoirs, washing drums, rinse chambers, wash basins or sinks, toilets, water tanks, toilet rims, siphons, water pipes and / or lines, water inlets and / or water outlets, in particular for preventing and / or reducing odors and / or for preventing and / or reducing and / or removing microbiologically induced deposits and / or for improving hygiene, characterized in that The agent contains dispersin, wherein the dispersin has β-1,6-N-acetylglucosaminidase activity and comprises an amino acid sequence that is at least 70%, and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identical to the amino acid sequence given in SEQ ID NO: 1 over its entire length, and is based on the method according to SEQ ID NO: NO:1 number, in the corresponding position 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71, 72, 74, 77, 79, 80, 81, 82, 90, 99, 100, 103, 104, 105, 106, 107, 110, 1 11, 113, 114, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 127, 128, 131, 135, 138, 139, 140, 142, 145, 147, 148, 149, 150, 151, 152, 163, 164, 167, 168, 170, 171, 173, 174, 175, 177, 178, 179, 180 1, 185, 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221, 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251, 252, 253, 254, 256, 260, 261, 262, 263, 264, 265 , 267, 268, 270, 271, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321 and 323 have at least one amino acid substitution and / or deletion.

9. The agent of claim 8, wherein the at least one amino acid substitution and / or deletion is selected from the group consisting of: D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19N, E19P, K22A, K22M, K22V, S23C, S23E, S23I, S23L, S23R, S23T, S23V, V25R, D26M, Y30*, Y30D, Y30L, Y30M, Y30N, Y3 0R, Y30T, Y30V, G32L, G32M, G32R, N43*, N43H, N43L, E44*, N45D, N45L, N45V , A49W, A49Y, Y52*, Y52M, G54L, G54M, G54N, S56T, S56W, S57W, E58N, N59A, N5 9C, N59D, N59E, N59F, N59M, N59R, N59V, N59W, T60V, N62C, N62D, N62H, N62Q , N62W, T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71H, L7 1N, L71R, L71V, L71W, S72*, S72C, S72D, S72E, S72F, S72G, S72I, S72M, S72N , S72R, S72T, S72Y, I74L, S77A, D79V, K80*, K80E, K80H, K80L, K80N, K80Q, K 80V, K80W, D81A, D81G, D81L, D81R, D81S, D81T, D81V, D81W, I82V, L90F, E99 Q, E99R, L100S, K103A, K103R, K104N, K104W, D105N, V106A, V106D, V106E, V1 06H, V106K, V106L, V106M, V106N, V106Q, V106W, V106Y, K107A, K107C, K107 L, K107M, K107T, K107V, K107W, N110M, N110R, N110V, D111A, D111E, D111M, D 111N, D111Q, D111R, D111V, D111W, V113T, T114C, T114S, Y116D, Y116N, Y11 6R, S117*, S117D, S117H, S117N, S117P, E118*, E118A, E118D, E118G, E118L,E119G、E119W、T120I、T120L、T120M、T120V、T120W、D122*、D122H、D122R、Y123W、Y124C、Y124I、Y124K、Y124L、Y124M、Y124Q、Y124R、Y124T、Y124V、Y124W、D125C、D125G、D125K、D125Q、D125R、N126V、R127D、R127H、R127K、R127L、R127M、R127Q、R127W、V128C、V128L、V128T、D131V、Q135*、Q135A、Q135D、Q135E、Q135K、Q135M、Q135Y、D138K、D138L、D138M、D138Q、D138R、D138S、D138V、D138W、E139W、D142R、D142W、Y145*、Y145H、Y145L、Y145N、Y145V、P147A、P147C、P147D、P147F、P147G、P147L、P147M、P147R、P147S、P147T、P147V、K148A、K148D、K148L、K148V、F149L、F149M、F149N、E150D、E150H、E150K、E150L、E150M、E150N、E150R、E150V、E150W、E150Y、G151A、G151C、G151D、G151L、G151N、G151P、G151S、G151W、K152D、K152L、K152R、G164D、G164E、G164H、G164S、G164V、V167D、V167E、V167L、V167P、V167Q、V167R、V167W、H168N、L170A、L170D、L170E、L170F、L170H、L170K、L170M、L170N、L170P、L170Q、L170R、L170S、L170V、L170W、L170Y、D171A、D171C、D171E、D171K、D171L、D171M、D171Q、D171R、D171V、D171W、D171Y、I173C、D174H、D174M、D174N、D174V、D174W、F175Y、N177M、Q178*、Q178A、Q178K、Q178R、Q178W、I179T、S181C、S181D、S181F、S181G、S181N、S181P、S181Q、S181T、S181V、S181W、E185M、E185R、E185V、E185W、S186D、S186E、S186H、S186I、S186K、S186L、S186M、S186N、S186Q、S186R、S186V、S186W、K187C、K187D、K187G、K187R、K187S、K187V、K187W、Y188P、E189L、E189V、E189W、S199C、S199L、S199M、S199Y、E200D、E200F、E200K、E200L、E200M、E200N、E200R、E200W、A203C、A203D、A203E、A203G、A203L、A203M、A203P、A203R、A203S、A203T、A203V、A203W、N204L、N204M、N204V、N204W、N204Y、L205I、D207A、D207C、D207E、D207G、D207N、D207Q、D207S、D207V、D207W、S208A、S208C、S208D、S208G、S208L、S208Q、S208T、S208V、S208W、S210T、Q215R、Q215M、Q215L、Q215*、S217V、T218A、T218L、T218Q、T218R、T218V、G222D、E224A、E224P、N227A、N227Q、N227R、N227S、N227T、N227K、D230*、D230R、D230T、D230W、E232D、E232V、N233H、N233Q、N233R、N233W、W234R、G235W、G235A、G235E、G235F、G235H、G235I、G235L、G235M、G235N、G235P、G235S、G235V、S237C、S237G、S237M、S237N、S237W、S237Y、Y244C、Y244E、Y244M、L249H、L249K、L249Q、L249R、L249W、L249Y、S251L、S251N、S251R、S251W、N252P、N252C、G253D、G253W、F254I、F254L、F254M、F254Y、Q256E、Q256R、N260*、N260A、N260C、N260E、N260I、N260K、N260L、N260M、N260Q、N260R、N260V、N260W、N260Y、E261*、E261A、E261D、E261R、E261W、Q262*、Q262F、Q262H、Q262W、Q262Y, M263K, M263L, M263Q, D264*, D264C, D264E, Y265F, N267S, N267T, W268C, W268E, W268M, W268R, Y270F, A271D, A271G, H272D, H272I, Y281P, Y282E, Y282N, Y281R, H272W, N273W, K274R, K274A, K274H, F276A, F276C, F276K, F276N, F 276G, F276L, F276M, F276P, F276S, F276V, F276W, I278A, I278K, I278N, I278Q, I278V, S279C, S279D, S279E, S279G, S 279N, D280C, D280E, Y281*, Y281A, Y281C, Y281H, Y281K, Y281N, Y281P, Y282E, Y282N, H283I, A284I, A284L, A284N, A2 84P, A284T, A284V, T287N, S288D, S288K, S288N, V290I, K291L, K291R, K291V, T296C, E300A, E300D, H301C, H301N, H3 01R, T303A, T303C, T303G, T303K, T303Q, T303R, T303W, D304C, D304M, L305M, L305N, S306C, K308A, K308D, K308G, K30 8I, K308L, K308Q, K308S, K308T, K308V, K308Y, K309A, K309C, K309D, K309H, K309L, K309M, K309N, K309Q, K309S, K309T, K309I, K312A, K312L, K312M, K312N, K312Q, K312S, K312W, E314I, E314L, E314V, L315I, L315V, R319A, N323R, and combinations thereof.

10. The reagent according to claim 8 or 9, wherein the reagent is substantially free of boron-containing compounds, preferably free of boron-containing compounds; and / or the pH of a 1 wt % solution of the reagent in deionized water at 20° C. is in the range of about 6 to about 11, in particular about 6.5 to about 10.5, more preferably about 7 to about 10, and particularly preferably about 8 to about 9.

11. A method for cleaning surfaces, in particular household surfaces, preferably washing machines or dishwashers, pump sump, tanks, cisterns, washing drums, rinse chambers, wash basins or sinks, toilets, water tanks, toilet rims, siphons, water pipes and / or lines, water inlets and / or water outlets, in particular for preventing and / or reducing odors and / or for preventing and / or reducing and / or removing microbiologically induced deposits and / or for improving hygiene, characterized in that In at least one process step, dispersin according to claim 1 or 2 or a reagent according to one of claims 8 to 10 is used, the process preferably being carried out in a temperature range of about 10° C. to about 60° C., preferably about 15° C. to about 40° C., particularly preferably about 20° C. to about 30° C.

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