A composite nanoemulsion, its preparation method and use

Abstract

This invention relates to a composite nanoemulsion, its preparation method, and its application. Each liter of the composite nanoemulsion comprises: 35g-50g of fat-soluble vitamins, 85g-100g of water-soluble vitamins, 2g-5g of high-lanolin selenium, 5g-25g of yucca extract, 15g-40g of nicotinamide, 40g-60g of urea, 140g-160g of emulsifier, 25g-45g of solubilizer, and 8g-15g of stabilizer. The composite nanoemulsion of this invention achieves the synergistic co-loading of fat-soluble vitamins, water-soluble vitamins, the natural functional ingredient yucca extract, and organic selenium. This composite nanoemulsion not only has more and higher levels of nutrients but also exhibits good stability, long shelf life, small particle size, and narrow distribution.

Description

Technical Field

[0001] This invention relates to the field of nanoemulsion technology, specifically to a composite nanoemulsion, its preparation method, and its application. Background Technology

[0002] Vitamins are essential micronutrients for animal growth, development, immune regulation, and metabolic function maintenance. In livestock, poultry, pet, and aquaculture, vitamin supplementation plays a crucial role in improving production performance, enhancing disease resistance, and improving animal health. However, traditional veterinary vitamin preparations (such as tablets and capsules) suffer from low bioavailability and poor stability, limiting their effectiveness. In recent years, nanoemulsion technology has gradually become a research hotspot in vitamin delivery systems due to its advantages such as improving the solubility of fat-soluble vitamins, enhancing absorption rates, and improving stability.

[0003] Currently, some veterinary vitamin nanoemulsion products have been launched, but existing technologies still have the following shortcomings:

[0004] First, the vitamin composition is limited and the content is insufficient. Currently, most veterinary vitamin nanoemulsions on the market primarily contain fat-soluble vitamins (such as vitamins A, D, E, and K), while water-soluble vitamins (such as B vitamins and vitamin C) are added in small amounts, resulting in an unbalanced nutritional profile. Furthermore, some products have low vitamin content, failing to achieve the desired nutritional supplementation effect.

[0005] Secondly, there is a lack of natural functional ingredients. Yucca extract (rich in saponins, polysaccharides, and other active ingredients) has multiple functions in animal nutrition, including reducing ammonia emissions, improving gut health, enhancing immunity, and providing antioxidant effects. However, existing veterinary vitamin nanoemulsions rarely contain such natural plant ingredients, failing to fully leverage their synergistic effects. Especially in intensive farming environments, they cannot effectively alleviate oxidative stress and gut health problems in animals.

[0006] Third, the key trace element selenium is not fortified. Selenium is an essential trace element for animals, crucial for improving reproductive performance, enhancing immunity, and boosting antioxidant capacity. However, existing nanoemulsion systems generally do not contain added selenium.

[0007] To address the above shortcomings, the applicant's experimental research revealed that simply increasing the amount of water-soluble and fat-soluble vitamins leads to a significant decrease in system stability, making phase separation or precipitation more likely and hindering the formation of a stable and homogeneous nanoemulsion system. While inorganic selenium exhibits good water solubility, it suffers from low bioavailability and potential toxicity, making it unsuitable for veterinary nanoemulsion formulations. Organic selenium, due to its complex molecular structure, demonstrates poor solubility and dispersibility in nanoemulsion systems, presenting significant technical obstacles. Furthermore, the saponin components in yucca extract, due to their surface-active properties, may interfere with the integrity of the nanoemulsion's interfacial membrane structure, leading to stability issues such as droplet aggregation or system demulsification. Summary of the Invention

[0008] The purpose of this invention is to provide a composite nanoemulsion, its preparation method, and its application. This composite nanoemulsion not only achieves synergistic high-content encapsulation of water-soluble and fat-soluble vitamins, but also innovatively integrates active ingredients such as organic selenium and yucca extract. While maintaining the stability of the system, it significantly improves the nutritional value and functional characteristics of the product.

[0009] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0010] This invention provides a composite nanoemulsion, wherein each liter of the composite nanoemulsion comprises: 35g-50g fat-soluble vitamins, 85g-100g water-soluble vitamins, 2g-5g selenium lanolin, 5g-25g yucca extract, 15g-40g nicotinamide, 40g-60g urea, 140g-160g emulsifier, 25g-45g solubilizer, and 8g-15g stabilizer, wherein: the fat-soluble vitamins include vitamin A, vitamin D, and vitamin E; the water-soluble vitamins... The soluble vitamins include vitamin B and vitamin C; the emulsifier is selected from at least two polyethylene glycol glycerol ricinoleate esters, wherein one polyethylene glycol glycerol ricinoleate ester has a polyethylene glycol chain degree of polymerization of 31-40, and the other polyethylene glycol glycerol ricinoleate ester has a polyethylene glycol chain degree of polymerization of 20-30, and the mass ratio of the polyethylene glycol glycerol ricinoleate ester with a polyethylene glycol chain degree of polymerization of 20-30 to the polyethylene glycol glycerol ricinoleate ester with a polyethylene glycol chain degree of polymerization of 31-40 is (3-12):1. Unless otherwise specified, the degree of polymerization (DP) in this invention refers to the number of repeating units in the polymer molecular chain, which is an important parameter describing the polymer chain length. Taking a polyethylene glycol chain degree of polymerization of 31-40 as an example, it means having 31-40 repeating -CH2-CH2-O- units.

[0011] This invention optimizes the performance of nanoemulsion systems through the synergistic mechanism of urea and nicotinamide. Specifically, the addition of urea effectively disrupts the hydrogen bond network between water molecules, significantly reducing the viscosity of the aqueous phase, thereby promoting the uniform dispersion of vitamin B2 and other components, inhibiting droplet aggregation, and maintaining the stability of the nanoemulsion. Simultaneously, nicotinamide molecules form stable molecular connections at the oil-water interface through bridging, ensuring the long-lasting dispersion stability of vitamin B2 and other components in the aqueous phase.

[0012] Furthermore, by optimizing the composition of the emulsion system and the organic selenium component, this invention significantly reduces the interfacial tension of the system, further promotes the formation of nano-sized droplets, improves the particle size distribution and storage stability of the nanoemulsion, and also increases the solubility of the oil phase component in the aqueous phase.

[0013] In some embodiments, the vitamin A content in the composite nanoemulsion is 4-7 g / L, meaning that each liter of composite nanoemulsion contains 4-7 g of vitamin A. Preferably, the vitamin A content in the composite nanoemulsion is 5-6.5 g / L.

[0014] In some embodiments, the vitamin D content in the composite nanoemulsion is 0.1~0.5 g / L, more preferably 0.2~0.4 g / L.

[0015] In some embodiments, the vitamin E content in the composite nanoemulsion is 30-40 g / L, more preferably 33-38 g / L.

[0016] In some embodiments, the vitamin B content in the composite nanoemulsion is 2-4 g / L, more preferably 2-3 g / L.

[0017] In some embodiments, the vitamin C content in the composite nanoemulsion is 80-100 g / L, more preferably 85-95 g / L.

[0018] In some preferred embodiments, the vitamin A is vitamin A palmitate.

[0019] In some preferred embodiments, the vitamin D is vitamin D3.

[0020] In some preferred embodiments, the vitamin E is vitamin E acetate.

[0021] In some preferred embodiments, the vitamin B is vitamin B2.

[0022] In some embodiments, the co-solvent is selected from one or more of polyethylene glycol and propylene glycol.

[0023] In some embodiments, the stabilizer is sodium metabisulfite, sodium bicarbonate, and ethylenediaminetetraacetic acid (EDTA). Preferably, the sodium metabisulfite content in the composite nanoemulsion is 0.5–2.5 g / L, more preferably 1–2 g / L. The sodium bicarbonate content in the composite nanoemulsion is 8–12 g / L, more preferably 9–11 g / L. The EDTA content in the composite nanoemulsion is 0.1–0.5 g / L, more preferably 0.2–0.4 g / L.

[0024] In some specific embodiments, ethylenediaminetetraacetic acid salt is disodium ethylenediaminetetraacetic acid.

[0025] In some embodiments, the average particle size of the composite nanoemulsion does not exceed 50 nm. Further, the average particle size of the composite nanoemulsion is 40 nm to 50 nm.

[0026] In some specific embodiments, each liter of the composite nanoemulsion comprises: 4g~7g vitamin A, 0.1g~0.5g vitamin D, 30g~40g vitamin E, 2g~4g vitamin B, 80g~100g vitamin C, 2g~5g high-lanolin selenium, 10g~20g yucca extract, 15g~25g nicotinamide, 45g~55g urea, 110g~120g polyethylene glycol glycerol ricinoleate with a degree of polymerization of 20~30, 10g~20g polyethylene glycol glycerol ricinoleate with a degree of polymerization of 31~40, 25g~45g cosolvent, 8g~12g sodium bicarbonate, 0.5g~2.5g sodium metabisulfite, and 0.1g~0.5g ethylenediaminetetraacetic acid.

[0027] In some embodiments, the composite nanoemulsion further includes xylooligosaccharides in a content of 10g to 20g.

[0028] In some embodiments, the dispersion medium of the composite nanoemulsion is water.

[0029] A second aspect of the present invention is to provide a method for preparing the composite nanoemulsion as described above, comprising the following steps:

[0030] (1) After mixing fat-soluble vitamins, add polyethylene glycol glycerol ricinoleate with a degree of polymerization of 20-30 and part of the dispersion medium, mix evenly, and prepare the first mixture;

[0031] (2) After mixing yucca extract with polyethylene glycol glycerol ricinoleate with a degree of polymerization of 31-40, add a cosolvent and part of the dispersion medium, mix evenly, and prepare a second mixture.

[0032] (3) Dissolve urea in the remaining dispersion medium, add stabilizer, water-soluble vitamin, nicotinamide and high lanolin selenium, mix well to make a third mixture;

[0033] (4) Mix the first mixture, the second mixture and the third mixture evenly to form the composite nanoemulsion.

[0034] In some embodiments, step (1) involves mixing the fat-soluble vitamins at 50-60°C.

[0035] In some embodiments, the mixing in steps (1) to (4) is carried out under stirring conditions, with stirring speeds of 300 to 500 r / min each.

[0036] A third aspect of the present invention is to provide an application of the composite nanoemulsion as described above, the application comprising adding the composite nanoemulsion as a feed additive to feed or drinking water.

[0037] Due to the application of the above technical solution, the present invention has the following advantages compared with the prior art:

[0038] The composite nanoemulsion of the present invention achieves synergistic co-loading of fat-soluble vitamins, water-soluble vitamins, natural functional ingredient yucca extract, and organic selenium. This composite nanoemulsion not only has more and higher nutritional content, but also has good stability, long shelf life, small particle size, and narrow distribution. Detailed Implementation

[0039] The present invention will be further described below with reference to embodiments. However, the present invention is not limited to the following embodiments. The implementation conditions used in the embodiments can be further adjusted according to different requirements of specific applications, and the implementation conditions not specified are conventional conditions in the industry. The technical features involved in the various embodiments of the present invention can be combined with each other as long as they do not conflict with each other.

[0040] Unless otherwise specified, the raw materials used in the following examples and comparative examples are all commercially available products.

[0041] Unless otherwise specified, “parts” in the following text refers to parts by weight.

[0042] Example 1: This example provides a composite nanoemulsion with the following components:

[0043] Vitamin E acetate (purchased from DSM Vitamins (Shanghai) Co., Ltd., production batch number 0420085) 35 parts, Vitamin A palmitate (purchased from Shangyu Xinhecheng Biochemical Co., Ltd., production batch number 0424070005) 5.88 parts, Vitamin D3 (purchased from Zhejiang Weishi Biotechnology Co., Ltd., production batch number 2936290090) 0.3 parts, Vitamin B2 (purchased from Shanghai Haijiano Pharmaceutical Development Co., Ltd., production batch number HS230909) 2.5 parts, Vitamin C (purchased from Shandong Luwei Pharmaceutical Co., Ltd., production batch number 2240123009) 90 parts, High-laminine selenium (purchased from Yinglian Pumeixin Technology (Jiangxi) Co., Ltd., brand name Pumeixin Interselenium) 3.5 parts of yucca extract (purchased from Xi'an Nuozhong Kangjian Biotechnology Co., Ltd., production batch number C25022101) 15 parts of nicotinamide 20.5 parts of xylooligosaccharide (purchased from Shandong Longli Biotechnology Co., Ltd., production batch number F95P20241036) 15 parts of urea 50 parts of sodium bicarbonate 10 parts of bulido 695 (polyethylene glycol glycerol ricinoleate, degree of polymerization of polyethylene glycol 26, purchased from Norinon) 130 parts of bulido 694 (polyethylene glycol glycerol ricinoleate, degree of polymerization of polyethylene glycol 36, purchased from Norinon) 15 parts of propylene glycol 35 parts of sodium metabisulfite 1.5 parts of disodium ethylenediaminetetraacetate (EDTA-2Na) 0.3 parts of purified water to a final volume of 1L.

[0044] This embodiment also provides a method for preparing composite nanoemulsions, including the following steps:

[0045] (1) Mix vitamin A palmitate, vitamin E acetate and vitamin D3, heat to 50~60℃, stir evenly at 300~500r / min, add Bridal 695 and continue stirring until even, then slowly add 15~20% purified water and continue stirring until clear and transparent, and prepare the first emulsion for later use.

[0046] (2) Mix yucca extract with bulido 694, stir well, then add propylene glycol and 2-5% purified water, stir well, and prepare a second emulsion for later use.

[0047] (3) Dissolve urea in the remaining purified water at 20°C. After it is completely dissolved, add sodium bicarbonate and vitamin B2 in sequence and stir thoroughly. Then add nicotinamide, and finally add vitamin C, selenium lanolinate, EDTA-2Na, sodium metabisulfite and xylooligosaccharide in sequence and stir thoroughly until completely dissolved to prepare an aqueous phase for later use.

[0048] (4) Mix the first emulsion, the second emulsion and the water phase, and stir for 10 to 15 minutes at room temperature (25°C) and a speed of 300 to 500 r / min to prepare a composite nanoemulsion.

[0049] Example 2: This example is largely the same as Example 1, except that the amount of vitamin B2 added to the composite nanoemulsion is different, which is 3.5 parts.

[0050] Example 3: This example is largely the same as Example 1, except that the composite nanoemulsion does not contain xylooligosaccharides.

[0051] Comparative Example 1: This comparative example is largely the same as Example 1, except that the composite nanoemulsion does not contain nicotinamide.

[0052] Comparative Example 2: This comparative example is largely the same as Example 1, except that the amount of nicotinamide added to the composite nanoemulsion is different, which is 10 parts.

[0053] Comparative Example 3: This comparative example is largely the same as Example 1, except that the composite nanoemulsion does not contain urea.

[0054] Comparative Example 4: This comparative example is largely the same as Example 1, except that the amount of urea added to the composite nanoemulsion is different, which is 30 parts.

[0055] Comparative Example 5: This comparative example is largely the same as Example 1, except that the amounts of Buridol 695, Buridol 694, and propylene glycol added to the composite nanoemulsion are different. Specifically, Buridol 695 is 100 parts, Buridol 694 is 5 parts, and propylene glycol is 10 parts.

[0056] Comparative Example 6: This comparative example is largely the same as Example 1, except that an equal amount of methionine selenium is used instead of high-lanolin selenium in the composite nanoemulsion.

[0057] Comparative Example 7: This comparative example is largely the same as Example 1, except that the composite nanoemulsion does not contain high levels of selenium lanolin, yucca extract, nicotinamide, and xylooligosaccharides.

[0058] Performance testing:

[0059] 1. Appearance: Observe the appearance and transparency of the sample after it has been left to stand.

[0060] The appearance results of the above embodiments and comparative examples are shown in Table 1.

[0061]

[0062] The product obtained in the embodiments of the present invention has a qualified appearance, while the product obtained in the comparative example failed to form a nanoemulsion.

[0063] 2. Particle size: Using a laser particle size analyzer, the nanoemulsion is diluted to the concentration required for the laser particle size analyzer test, and the particle size distribution and average particle size are calculated by measuring light scattering.

[0064] The particle sizes of the above embodiments are shown in Table 2.

[0065]

[0066] The nanoemulsions prepared in the embodiments of the present invention have small particle size and narrow particle size distribution, which can be absorbed more quickly, thereby reducing the degradation and loss of active ingredients in the gastrointestinal tract and helping to improve the bioavailability of nanoemulsions.

[0067] 3. Physical stability:

[0068] 1) Centrifugation experiment: Under room temperature conditions, the nanoemulsion was diluted 5 times with deionized water and placed in a centrifuge. It was centrifuged at 3000 r / min for 15 min, 30 min and 45 min respectively. The presence or absence of stratification and precipitation was observed.

[0069] The centrifugation results of the above embodiments and comparative examples are shown in Table 3.

[0070]

[0071] The nanoemulsion prepared in Example 1 of this invention exhibits good centrifugal stability. In contrast, the product in the comparative example showed precipitation or stratification after centrifugation, indicating poor centrifugal stability.

[0072] 2) Dilution stability: The nanoemulsion was diluted at different ratios, and its stability was observed. Specifically, the nanoemulsion was diluted 10, 20, and 30 times, and the particle size and polymer dispersibility index (PDI) were measured using a laser particle size analyzer. When the average particle size change before and after dilution was ≤10% and the PDI after dilution was ≤0.2, it indicated that the nanoemulsion had good dilution stability.

[0073] Table 4 below shows the average particle size and PDI changes of the nanoemulsion of Example 1 at different dilution factors.

[0074]

[0075] As can be seen, the average particle size of the nanoemulsion in Example 1 remained almost unchanged and the PDI changed little when diluted 10 to 30 times, indicating that the nanoemulsion in Example 1 has good dilution stability.

[0076] The nanoemulsions of Example 2 and Example 3 were examined using the same method and showed similar experimental results.

[0077] 3) High temperature test: The nanoemulsion was placed at 25℃, 40℃, and 60℃ and 75% humidity and observed in the dark for 5 days and 10 days respectively. The particle size and PDI were measured by laser particle size analyzer and compared with the particle size and PDI before heat treatment. The smaller the change, the better the storage stability. When the average particle size change before and after heat treatment is ≤10% and the PDI after dilution is ≤0.2, it indicates that the nanoemulsion has good heat treatment stability.

[0078] Table 5 below shows the average particle size and PDI variation of the nanoemulsion of Example 1 at different temperatures.

[0079]

[0080] As can be seen, the nanoemulsion of Example 1 exhibits relatively small changes in average particle size and PDI at room temperature, indicating its good stability at room temperature. However, it is still sensitive to temperature; at 40℃, 60℃, and high humidity, aggregation (i.e., particle size increase) increases with time, leading to increased risk. It should be noted that the average particle size at day 0 here differs slightly from that in Table 2, which may be due to measurement errors.

[0081] The nanoemulsions of Example 2 and Example 3 were examined using the same method and showed similar experimental results.

[0082] 4) Low temperature test: The nanoemulsion was placed in a 4℃ refrigerator for 20 days and 40 days respectively. Its appearance, particle size and PDI were observed. When the average particle size change before and after low temperature treatment was ≤10% and the PDI after dilution was ≤0.2, it indicated that the nanoemulsion had good low temperature stability.

[0083] Table 6 below shows the average particle size and PDI changes of the nanoemulsion of Example 1 under low-temperature testing.

[0084]

[0085] As can be seen, the average particle size and PDI of the nanoemulsion in Example 1 did not change significantly after being placed at low temperature for 20 days or even 40 days, indicating that the nanoemulsion has good low temperature stability.

[0086] The nanoemulsions of Example 2 and Example 3 were examined using the same method and showed similar experimental results.

[0087] 4. Chemical stability:

[0088] 1) High-temperature test:

[0089] The nanoemulsions were stored at 25℃, 40℃, and 60℃ in the dark for 30 days, respectively, to investigate the retention rates (g / L) of vitamin A palmitate and vitamin E acetate in the nanoemulsions. Vitamin A palmitate and vitamin E acetate were detected by Agilent high-performance liquid chromatography (HPLC). Pure methanol was used as the mobile phase for vitamin A palmitate, and a mixture of methanol and purified water was used as the mobile phase for vitamin E acetate. Detection conditions were consistent with existing techniques.

[0090] Table 7 below shows the changes in the retention rates (g / L) of vitamin A palmitate and vitamin E acetate of the nanoemulsion in Example 1 at different temperatures.

[0091]

[0092] Note: Some measurements of vitamin A palmitate in the table are higher than 5.88 g / L, which may be due to measurement error. It is generally considered that 96% to 104% of the labeled amount is normal.

[0093] As can be seen, the vitamin A palmitate and vitamin E acetate of the nanoemulsion in Example 1 did not change significantly at different temperatures, indicating that the nanoemulsion has good chemical stability.

[0094] The nanoemulsions of Example 2 and Example 3 were examined using the same method and showed similar experimental results.

[0095] The nanoemulsion was stored at 40°C in the dark for 1 month, 3 months, and 6 months, and samples were taken periodically to investigate the retention rate of vitamin B2 in the nanoemulsion. Vitamin B2 was detected by fluorescence spectrophotometry, and the detection conditions were in accordance with existing techniques.

[0096] The test results for each embodiment are shown in Table 8 below.

[0097]

[0098] It is evident that even at high temperatures (40°C) and with prolonged storage, the vitamin B2 retention rate of the nanoemulsion in this embodiment of the invention showed almost no significant change, indicating that the nanoemulsion also exhibits good storage stability at high temperatures.

[0099] 2) Shelf life: The nanoemulsion is sealed and stored in the dark at room temperature (25℃). The appearance, particle size, zeta potential and other indicators of the nanoemulsion are tested regularly. At the same time, it is observed whether there are phenomena such as layering, precipitation and deterioration, until the emulsion no longer meets the quality requirements, so as to determine its shelf life.

[0100] The performance test data of the above embodiments and comparative examples are summarized in Table 9 below.

[0101]

[0102] It is evident that the nanoemulsions of the present invention have a longer shelf life.

[0103] This invention not only boasts a high content of active ingredients, approaching 20%, but also achieves highly efficient co-loading of multiple active ingredients, including vitamin E acetate, vitamin A palmitate, vitamin D3, vitamin B2, vitamin C, high-laminine selenium, yucca extract, niacinamide, and xylooligosaccharides. This unique co-loading method significantly enhances the functional density and application value of the product, giving it broad application prospects in related fields.

[0104] Furthermore, by optimizing the formulation, this invention produces a nanoemulsion that is a clear and transparent solution with small particle size and narrow distribution, while also exhibiting excellent physical and chemical stability. These characteristics make the product more convenient and reliable during storage, transportation, and use, further enhancing its market competitiveness.

[0105] Comparing Example 1 and Comparative Example 1 reveals that without the addition of nicotinamide, nanoemulsions cannot be formed when multiple active ingredients, such as vitamin B2, are added. This is likely because nicotinamide is an amphiphilic molecule containing a hydrophilic amide group and a hydrophobic pyridine ring in its molecular structure. This unique structure allows nicotinamide to adsorb at the oil-water interface, effectively reducing interfacial tension and thus promoting the formation and stabilization of nanoemulsion droplets. Furthermore, the amide group of nicotinamide can form a hydrogen bond network with other components in the system (e.g., vitamin B2), significantly enhancing the mechanical strength of the interfacial film; its pyridine ring may interact with hydrophobic components (e.g., vitamin E) through π-π stacking interactions, further stabilizing the droplet structure. However, insufficient nicotinamide will also prevent the formation of stable nanoemulsions (as shown in Comparative Example 2); while excessive amounts will have adverse effects on the system. Therefore, the preferred content of nicotinamide in the nanoemulsion is 15-40 g / L, more preferably 15-30 g / L.

[0106] Comparing Example 1 and Comparative Example 3 reveals that without the addition of urea, nanoemulsions cannot be formed even with the addition of multiple active components such as vitamin B2. This is likely because urea synergistically promotes the dissolution of components such as vitamin B2 under weakly alkaline conditions, facilitating their dispersion in the medium and effectively reducing droplet aggregation. Simultaneously, urea can indirectly maintain the conformation of some macromolecules (such as yucca extract), preventing their aggregation due to hydrophobic interactions and weakening the negative charge of high-lanolin selenium. Furthermore, urea can synergistically maintain the stability of the nanoemulsion system with other components within the system. However, insufficient urea will also prevent the formation of stable nanoemulsions (as shown in Comparative Example 2); while excessive urea will have adverse effects on the system. Therefore, the preferred urea content in the nanoemulsion is 40-60 g / L, more preferably 45-55 g / L.

[0107] Furthermore, emulsifiers and cosolvents are also crucial in this invention. When the amounts of emulsifiers and cosolvents added are inappropriate (as in Comparative Example 5), not only will nanoemulsions fail to form, but the stability of the system will also be significantly compromised. The choice of organoselenium is equally important. When methionine selenium is used, its poor solubility in the aqueous phase hinders the formation of a stable nanoemulsion system.

[0108] Application testing:

[0109] Three hundred 12-day-old AA white-feathered broiler chickens were randomly divided into three representative groups: a control group, test group 1, and test group 2. The chickens were housed in four-layer stacked cages with free access to water and feed throughout the experiment. The control group was fed a basal diet, while test groups 1 and 2 were fed a basal diet supplemented with nanoemulsions from Example 1 and Comparative Example 7, respectively, at a dosage of 500 g / t of diet. The experiment lasted 14 days, including a 7-day pre-trial period and a 7-day formal trial period. During the experiment, the feed troughs were emptied before each feeding to ensure the feed was completely used.

[0110] After the experiment, the indicators were measured, and the test results are shown in Table 10 below. Among them:

[0111] Average daily weight gain = (total weight at the end of the period for chickens in the group - initial total weight for chickens in the group) / (number of days in the experiment × total number of chickens).

[0112] Average daily feed intake = Total feed intake of chickens in the group / (Number of days in the experiment × Total number of chickens).

[0113] Feed conversion ratio = daily feed intake / daily weight gain. The lower the feed conversion ratio, the less feed is required per unit of weight gain, and the higher the breeding efficiency.

[0114] Survival rate = [(Total number of chickens in the group - Number of dead chickens in the group / Total number of chickens in the group)] × 100%.

[0115] Uniformity = Number of qualified individuals / Total number of samples × 100%. Samples are drawn proportionally according to the size of the flock, with a minimum of 50 birds per sample. Qualified samples are defined as chickens whose weight is within ±10% of the average weight of the entire flock.

[0116]

[0117] As can be seen, the nanoemulsion used in this embodiment of the invention achieves optimal growth rate and feed conversion ratio in broilers; furthermore, it results in higher survival rate and uniformity of broilers. This demonstrates that the nanoemulsion of this invention can effectively promote healthy growth in broilers and has significant application value in improving breeding efficiency.

[0118] The present invention has been described in detail above, with the aim of enabling those skilled in the art to understand and implement the invention. However, this description should not be construed as limiting the scope of protection of the invention. All equivalent changes or modifications made in accordance with the spirit and essence of the invention should be included within the scope of protection of the invention.

Claims

1. A composite nanoemulsion, characterized in that, Each liter of composite nanoemulsion includes: 35g~50g fat-soluble vitamins, 85g~100g water-soluble vitamins, 2g~5g high-lanolin selenium, 5g~25g yucca extract, 15g~40g nicotinamide, 40g~60g urea, 140g~160g emulsifier, 25g~45g solubilizer, and 8g~15g stabilizer, of which: The fat-soluble vitamins include vitamin A, vitamin D, and vitamin E; The water-soluble vitamins include vitamin B and vitamin C; The emulsifier is selected from at least two polyethylene glycol glycerol ricinoleate, wherein one polyethylene glycol glycerol ricinoleate has a polyethylene glycol chain degree of polymerization of 31-40, and the other polyethylene glycol glycerol ricinoleate has a polyethylene glycol chain degree of polymerization of 20-30. The mass ratio of polyethylene glycol glycerol ricinoleate with a polyethylene glycol chain degree of polymerization of 20-30 to polyethylene glycol glycerol ricinoleate with a polyethylene glycol chain degree of polymerization of 31-40 is (3-12):

1. The composite nanoemulsion also includes a dispersion medium.

2. The composite nanoemulsion according to claim 1, characterized in that, The vitamin A content in the composite nanoemulsion is 4~7 g / L; And / or, the vitamin D content in the composite nanoemulsion is 0.1~0.5 g / L; And / or, the vitamin E content in the composite nanoemulsion is 30~40g / L; And / or, the vitamin B content in the composite nanoemulsion is 2~4 g / L; And / or, the vitamin C content in the composite nanoemulsion is 80~100g / L.

3. The composite nanoemulsion according to claim 1, characterized in that, The vitamin A is vitamin A palmitate; And / or, the vitamin D is vitamin D3; And / or, the vitamin E is vitamin E acetate; And / or, the vitamin B is vitamin B2.

4. The composite nanoemulsion according to claim 1, characterized in that, The co-solvent is selected from one or more of polyethylene glycol and propylene glycol; And / or, the stabilizer is sodium metabisulfite, sodium bicarbonate, and ethylenediaminetetraacetate.

5. The composite nanoemulsion according to claim 4, characterized in that, The sodium metabisulfite content in the composite nanoemulsion is 0.5~2.5 g / L; And / or, the sodium bicarbonate content in the composite nanoemulsion is 8~12 g / L; And / or, the content of ethylenediaminetetraacetic acid salt in the composite nanoemulsion is 0.1~0.5 g / L.

6. The composite nanoemulsion according to claim 1, characterized in that, The average particle size of the composite nanoemulsion does not exceed 50 nm.

7. The composite nanoemulsion according to claim 6, characterized in that, The average particle size of the composite nanoemulsion is 40 nm to 50 nm.

8. The composite nanoemulsion according to claim 1, characterized in that, Each liter of the composite nanoemulsion comprises: 4g~7g vitamin A, 0.1g~0.5g vitamin D, 30g~40g vitamin E, 2g~4g vitamin B, 80g~100g vitamin C, 2g~5g high-lanolin selenium, 10g~20g yucca extract, 15g~25g nicotinamide, 45g~55g urea, 110g~120g polyethylene glycol glycerol ricinoleate with a degree of polymerization of 20~30, 10g~20g polyethylene glycol glycerol ricinoleate with a degree of polymerization of 31~40, 25g~45g cosolvent, 8g~12g sodium bicarbonate, 0.5g~2.5g sodium metabisulfite, and 0.1g~0.5g ethylenediaminetetraacetic acid.

9. The composite nanoemulsion according to claim 1 or 8, characterized in that, The composite nanoemulsion also includes xylooligosaccharides in a content of 10g to 20g.

10. The composite nanoemulsion according to claim 1, characterized in that, The dispersion medium for the composite nanoemulsion is water.

11. The method for preparing the composite nanoemulsion according to any one of claims 1 to 10, characterized in that, Includes the following steps: (1) After mixing fat-soluble vitamins, add polyethylene glycol glycerol ricinoleate with a degree of polymerization of 20-30 and part of the dispersion medium, mix evenly, and prepare the first mixture; (2) After mixing yucca extract with polyethylene glycol glycerol ricinoleate with a degree of polymerization of 31-40, add a cosolvent and part of the dispersion medium, mix evenly, and prepare a second mixture. (3) Dissolve urea in the remaining dispersion medium, add stabilizer, water-soluble vitamin, nicotinamide and high lanolin selenium, mix well to make a third mixture; (4) Mix the first mixture, the second mixture and the third mixture evenly to form the composite nanoemulsion.

12. The application of the composite nanoemulsion as described in any one of claims 1 to 10, characterized in that, The applications include adding the composite nanoemulsion as a feed additive to feed or drinking water.

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