A device for detecting metabolomics biomarkers in bladder cancer

Abstract

This invention relates to the field of cell detection equipment technology, specifically to a detection device for bladder cancer metabolomics biomarkers. The device includes an ELISA reader, a moving stage, and well plates housed within it. It also includes a defoamer mounted on the outer wall of the ELISA reader. The defoamer comprises a fixing part, a pair of transmission parts, and a striking part and an air blowing part disposed within the fixing part. This bladder cancer metabolomics biomarker detection device, through a mechanical linkage design between the moving stage and the defoamer, uses a gear-driven toothed plate to move a dial-driven bending plate up and down, causing a striking rod to strike the well plate. Combined with synchronous airflow from the air blowing part, a protrusion pushes a piston plate within a second groove, directing airflow from the nozzle. This achieves automated removal of air bubbles from the wells before ELISA reader detection. The dual defoaming effect of striking vibration and airflow impact eliminates optical interference, ensuring the accuracy of the detection values ​​and simplifying the detection process.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cell detection equipment, in particular to a detection equipment for bladder cancer metabolomics markers. BACKGROUND

[0002] Existing data shows that high expression of PSMB5 is an independent risk prognostic factor for OS of bladder cancer patients. In order to clarify the influence of PSMB5 on the phenotype of bladder urothelial carcinoma cells, the proliferation of bladder urothelial carcinoma cells is detected by CCK8 method. The detection is usually based on enzyme-linked immunosorbent assay (ELISA), colorimetric method or fluorescence method, etc., and is carried out in a microplate. The enzyme labeler can accurately detect the weak color change in the microhole produced by the marker-enzyme reaction, realize the accurate quantitative analysis of trace metabolic markers, and is the key equipment in the detection.

[0003] The patent with application number CN202422147576.2 discloses a novel enzyme labeler based on a grating module and a filter module, which comprises an electric enzyme labeler body, a grating module, a filter module, a motor speed reduction mechanism, and further comprises a detection mechanism, a first detection control circuit and a second detection control circuit. The motor speed reduction mechanism is installed at the rear side end of the detection chamber, and the front side end of the rotating shaft of the motor speed reduction mechanism is provided with a supporting rod and a connecting rod. The rear side ends of the grating module and the filter module are installed together with the front side ends of the connecting rods. This brings convenience to the staff and saves the equipment fund input of the relevant departments.

[0004] The existing enzyme labeler lacks an automatic bubble removal structure during detection, and usually needs manual bubble removal, which is not only low in operation efficiency but also insufficient in removal rate, and it is difficult to remove stubborn bubbles adhering to the hole wall, which causes light path interference and causes deviation of OD value. At the same time, external centrifugation or pipetting operation forces the cells to separate from the constant temperature environment, causing degradation of formazan dye, and significantly amplifying the data fluctuation within the group. In addition, the fragmented bubble removal process will prolong the sample exposure time, which seriously restricts the statistical efficiency of the bladder cancer cell phenotype research.

[0005] In view of this, a detection equipment for bladder cancer metabolomics markers is proposed. SUMMARY

[0006] The present application aims to provide a detection equipment for bladder cancer metabolomics markers to solve the problems raised in the background.

[0007] To achieve the above-mentioned purpose, the present application provides the following technical scheme:

[0008] A kind of detection equipment of bladder cancer metabolomics marker, including microplate reader, mobile station and the hole plate placed in it, further including bubble remover arranged on the outer wall of microplate reader, the bubble remover includes fixed part, a pair of transmission parts and the knocking part and air blowing part arranged in the fixed part;

[0009] The mobile station includes a table plate and a tooth plate arranged on the left and right sides of the table plate.

[0010] The transmission part includes a dial, a gear arranged on the end of the central shaft and a lever arranged on the outer wall of the dial near the edge.

[0011] This setting, when the table plate is retracted into the microplate reader, the tooth plate engages the gear to drive the dial to rotate.

[0012] The knocking part includes a pair of bent plates, a lifting plate arranged on the top surface of the pair of bent plates, a plurality of knocking rods distributed on the bottom surface of the lifting plate, and a protrusion arranged on the front wall of the lifting plate.

[0013] When the dial rotates, the lever contacts the bottom horizontal plate of the bent plate, which drives it to move up, and after it separates from the bent plate, the bent plate falls back to its original position. The knocking rod knocks the hole plate surface, causing the bubbles to detach from the hole wall and float up and break.

[0014] The air blowing part includes a frame plate, a piston plate moving with it, a gas cylinder sleeved outside the piston plate, and a nozzle with a plurality of gas pipes at the bottom. The frame plate has a second sliding groove inside.

[0015] When the lifting plate moves up and down, the protrusion moves in the second sliding groove, driving the frame plate to move left and right in the gas cylinder with the piston plate. The gas is sent into the nozzle through the pipeline and blown into the hole of the hole plate through the gas pipes, eliminating unbroken bubbles.

[0016] In the technical solution of the present application, the table plate is connected to the electric push rod inside the microplate reader for controlling its movement. A placing groove for placing the hole plate is formed on the top surface of the table plate. A plurality of recesses for providing a falling interval for the internal structure of the air blowing part are arranged on the left and right sides of the placing groove on the top surface of the table plate. The tooth plate is fixedly connected to the outer wall of the table plate by screws.

[0017] This design makes the mobile station generate power when moving, which can drive the internal structure of the bubble remover to move, thereby eliminating the bubbles in the hole of the hole plate.

[0018] In the technical solution of the present application, the fixed part includes a fixed box, an upper partition plate connected to the inner wall of the fixed box, and an inner partition plate connected to the bottom surface of the upper partition plate on the left and right ends.

[0019] The upper partition plate is provided with a through groove penetrating from top to bottom at both left and right ends, the outer wall of the inner partition plate is provided with a rotating groove, and the inner wall of the inner partition plate is provided with a first sliding groove for providing a sliding interval for the internal structure of the air blowing part.

[0020] The fixed box is divided into spaces by the design of the upper partition plate and the inner partition plate.

[0021] The dial is rotationally connected to the inside of the rotating groove, the end center shaft of the dial extends to the inside of the inner partition plate, the gear is clamped and fixed at the end of the center shaft of the dial, and the dial rod is integrally formed with the dial.

[0022] The power generated when the moving table moves is transmitted by the engagement of the gear plate and the gear.

[0023] The end of the bottom transverse plate of the bending plate extends to the center of the dial, the bottom surface is located above the dial rod, the longitudinal plate body is slidingly connected to the inside of the first sliding groove and the end is clamped to the bottom surface of the lifting plate, and the knocking rod and the protruding block are both hot melt connected to the outer wall of the lifting plate.

[0024] The top surface of the bending plate is also clamped and fixed with a plurality of regularly distributed telescopic rods and springs sleeved outside the telescopic rods, the top end of the telescopic rod is clamped and fixed to the inside top surface of the fixed box, and the spring provides an elastic force to push the lifting plate to move downward.

[0025] The physical knocking method of the knocking rod knocking the hole plate surface eliminates the bubbles on the hole wall, reduces the link of manual bubble elimination, and simplifies the detection process.

[0026] The frame plate is slidingly connected to the top surface of the upper partition plate, a round rod is clamped and fixed between the end of the frame plate and the piston plate, and the piston plate is slidingly connected to the inside of the air cylinder.

[0027] The air cylinder is fixedly connected to the top surface of the upper partition plate by a screw, the upper and lower ends of the end of the air cylinder are respectively provided with an air inlet valve and an air outlet valve, a hose is clamped outside the air outlet valve of the air cylinder, and the other end of the hose is clamped and fixed with the spray head.

[0028] The lower side of the spray head is also provided with a square plate slidingly connected to the inside of the first sliding groove, the bottom surface of the two ends of the square plate is clamped with a lower protruding rod, a plurality of air nozzles sleeved in the air pipe at the bottom of the spray head are clamped and fixed in the inside of the square plate, and the end of the lower protruding rod is provided with a chamfer.

[0029] The above arrangement makes the air flow act on the bubble gathering area in close proximity, realizes local strengthening removal for stubborn bubbles, improves the removal rate of adherent bubbles, and provides a reliable basis for statistical analysis of the influence of PSMB5 phenotype.

[0030] Compared with the prior art, the present application has the following beneficial effects:

[0031] 1. The bladder cancer metabolomics marker detection device is automatically removed from the bubble in the hole before the detection of the enzyme label instrument through the mechanical linkage design of the mobile station and the bubble remover, the toothed plate engages the gear, the dial drives the bending plate to move up and down, the knocking rod is knocked, the synchronous airflow of the air blowing part is combined, the convex block is used to push the piston plate in the second sliding groove, the nozzle is directional blowing, the automatic removal of the bubble in the hole before the detection of the enzyme label instrument is realized, the double bubble removal of knocking vibration and airflow impact is used to eliminate optical interference, ensure the accuracy of the detection value, and simplify the detection process.

[0032] 2. The bladder cancer metabolomics marker detection device uses the displacement of the table plate to trigger the dynamic positioning of the air nozzle, when the groove moves to the lower side of the lower convex rod, the square plate slides down along the first sliding groove, the air nozzle is inserted into the hole, the airflow acts on the bubble gathering area in close proximity, strengthens the removal of stubborn bubbles, improves the removal rate of adherent bubbles, and guarantees the uniformity of the bladder cancer cell proliferation data in the high serum environment, providing a reliable basis for statistical analysis of the influence of PSMB5 phenotype. BRIEF DESCRIPTION OF DRAWINGS

[0033] Figure 1 It is a schematic diagram of the overall structure of the present application;

[0034] Figure 2 It is a schematic diagram of part of the structure of the present application;

[0035] Figure 3 It is a schematic diagram of the structure of the mobile station in the present application;

[0036] Figure 4 It is a schematic diagram of the structure of the bubble remover in the present application;

[0037] Figure 5 It is a schematic diagram of the structure of the bubble remover in the present application;

[0038] Figure 6 It is a schematic diagram of the structure of the bubble remover in the present application;

[0039] Figure 7 It is a schematic diagram of the structure of the fixed part in the present application;

[0040] Figure 8 It is a schematic diagram of the structure of the transmission part in the present application;

[0041] Figure 9 It is a schematic diagram of the structure of the knocking part in the present application;

[0042] Figure 10 Structure diagram of the air blowing part in the application;

[0043] Figure 11 Structure diagram of the air blowing part in the application;

[0044] Explanation of reference signs:

[0045] 100, enzyme label instrument;

[0046] 200, mobile station; 210, table plate; 211, placing groove; 212, recess; 220, tooth plate;

[0047] 300, hole plate;

[0048] 400, bubble remover; 410, fixed part; 411, fixed box; 412, upper partition plate; 4120, through groove; 413, inner partition plate; 4130, turning groove; 4131, first sliding groove; 420, transmission part; 421, dial; 422, gear; 423, dial lever; 430, knocking part; 431, bent plate; 432, lifting plate; 433, knocking lever; 434, protrusion; 435, telescopic lever; 436, spring; 440, air blowing part; 441, frame plate; 4410, second sliding groove; 442, round rod; 443, piston plate; 444, air cylinder; 445, hose; 446, nozzle; 447, square plate; 448, lower protrusion; 449, air nozzle. DETAILED DESCRIPTION

[0049] The technical solutions in the application will be described clearly and completely below in combination with the drawings in the application. Obviously, the described embodiments are only some of the embodiments of the application, but not all the embodiments. Based on the embodiments in the application, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the protection scope of the application.

[0050] Please refer to Figures 1-3 The embodiment provides a technical solution:

[0051] A detection device for bladder cancer metabolomics markers comprises an enzyme label instrument 100, a mobile station 200 and a hole plate 300 placed in the mobile station 200, and further comprises a bubble remover 400 arranged on the outer wall of the enzyme label instrument 100. The bubble remover 400 comprises a fixed part 410, a pair of transmission parts 420, and a knocking part 430 and an air blowing part 440 arranged in the fixed part 410.

[0052] Specifically, the mobile station 200 comprises a table plate 210 and tooth plates 220 arranged on the left and right sides of the table plate 210.

[0053] Further, the platform 210 is fixedly connected with the electric push rod inside the enzyme marker 100 for controlling the movement thereof, the top surface of the platform 210 is provided with a placing groove 211 for placing the hole plate 300, and a plurality of grooves 212 are provided on the left and right sides of the placing groove 211 on the top surface of the platform 210 for providing a falling interval for the internal structure of the air blowing part 440, and the tooth plate 220 is fixedly connected to the outer wall of the platform 210 through screws.

[0054] Further, after the hole plate 300 is completed, the hole plate 300 is placed in the placing groove 211 of the platform 210, the enzyme marker 100 is started, and after the parameters are set, the moving platform 200 is recovered to the inside thereof for detecting the hole plate 300, and the design of the grooves 212 and the tooth plate 220 enables the moving platform 200 to generate power when moving as a whole, so as to drive the internal structure of the bubble remover 400 to move, thereby eliminating the bubbles in the holes of the hole plate 300.

[0055] Please refer to Figures 2-7 In the embodiment, the fixing part 410 includes a fixing box 411, an upper partition plate 412 fixedly connected to the inner wall of the fixing box 411, and an inner partition plate 413 fixedly connected to the bottom surface of the left and right ends of the upper partition plate 412.

[0056] Specifically, the left and right ends of the upper partition plate 412 are provided with through grooves 4120 penetrating the upper and lower portions thereof, the outer wall of the inner partition plate 413 is provided with a rotating groove 4130, and the inner wall thereof is provided with a first sliding groove 4131 for providing a sliding interval for the internal structure of the air blowing part 440.

[0057] Further, the fixing box 411 is used for providing a placing interval for the transmission part 420, the knocking part 430 and the air blowing part 440, and the design of the upper partition plate 412 and the inner partition plate 413 divides the internal space of the fixing box 411.

[0058] Please refer to Figures 2-6 In the embodiment, the transmission part 420 includes a dial 421, a gear 422 provided at the end of the central shaft of the dial 421, and a dial rod 423 provided at the outer wall of the dial 421 close to the edge, and when the platform 210 is retracted into the enzyme marker 100, the tooth plate 220 engages the gear 422 to drive the dial 421 to rotate.

[0059] Specifically, the dial 421 is rotatably connected to the inside of the rotating groove 4130, the end of the central shaft thereof extends to the inside of the inner partition plate 413, the gear 422 is fixedly connected to the end of the central shaft of the dial 421, and the dial rod 423 is integrally formed with the dial 421.

[0060] Further, during the movement of the moving table 200, the toothed plate 220 engages the gear 422 to drive the dial plate 421 to rotate and continuously adjust the position of the dial rod 423. The power generated during the movement of the moving table 200 is transmitted through the engagement of the toothed plate 220 and the gear 422.

[0061] Please refer to Figures 2-9 As shown in the figure, in the embodiment, the knocking part 430 includes a pair of bent plates 431, a lifting plate 432 arranged on the top surface of the pair of bent plates 431, a plurality of knocking rods 433 distributed on the bottom surface of the lifting plate 432, and a protrusion 434 arranged on the front wall of the lifting plate 432. When the dial plate 421 rotates and the dial rod 423 is in contact with the bottom horizontal plate of the bent plate 431, the bent plate 431 is lifted up. After the dial rod 423 is separated from the bent plate 431, the bent plate 431 falls back to the original position. The knocking rod 433 knocks the surface of the hole plate 300, so that the bubbles are separated from the hole wall and float up to break.

[0062] Specifically, the end of the bottom horizontal plate of the bent plate 431 extends to the center of the dial plate 421, the bottom surface is above the dial rod 423, the longitudinal plate body is slidingly connected in the first sliding groove 4131 and the end is clamped to the bottom surface of the lifting plate 432. The knocking rod 433 and the protrusion 434 are both hot-melt connected to the outer wall of the lifting plate 432.

[0063] Further, a plurality of regular distribution of telescopic rods 435 and springs 436 sleeved outside the telescopic rods 435 are clamped and fixed on the top surface of the bent plate 431. The top end of the telescopic rod 435 is clamped and fixed to the inner top surface of the fixed box 411. The spring 436 provides a downward moving force to the lifting plate 432.

[0064] Further, when the dial plate 421 rotates and the dial rod 423 is in contact with the bottom horizontal plate of the bent plate 431, the bent plate 431 is lifted up. After the dial rod 423 is separated from the bent plate 431, the spring 436 is contracted and falls back to the original position by its own elastic force. The knocking rod 433 knocks the surface of the hole plate 300, so that the bubbles are separated from the hole wall and float up to break. This setting eliminates the bubbles on the hole wall by physical knocking of the knocking rod 433 on the surface of the hole plate 300, reduces the link of manual bubble elimination, and simplifies the detection process.

[0065] Please refer to Figures 2-11 As shown in the figure, in the embodiment, the blowing part 440 includes a frame plate 441, a piston plate 443 moving with the frame plate 441, an air cylinder 444 sleeved outside the piston plate 443, and a nozzle 446 provided with a plurality of air pipes at the bottom. The second sliding groove 4410 is arranged in the inner part of the frame plate 441. When the lifting plate 432 moves up and down, the protrusion 434 moves in the second sliding groove 4410, drives the frame plate 441 to move left and right in the air cylinder 444, sends the gas into the nozzle 446 through the pipeline, and blows the unbroken bubbles into the hole of the hole plate 300 through the air pipes.

[0066] Specifically, the frame plate 441 is slidingly connected to the top surface of the upper partition plate 412, and a round rod 442 is fixedly connected between the end of the frame plate 441 and the piston plate 443, and the piston plate 443 is slidingly connected to the inside of the air cylinder 444.

[0067] Further, the air cylinder 444 is fixedly connected to the top surface of the upper partition plate 412 by a screw, and the upper and lower ends of the end of the air cylinder 444 are respectively provided with an air inlet valve and an air outlet valve, and a hose 445 is fixedly connected to the outside of the air outlet valve of the air cylinder 444, and the other end of the hose 445 is fixedly connected to a spray head 446.

[0068] Further, a square plate 447 slidingly connected to the inside of the first sliding groove 4131 is further arranged below the spray head 446, lower protruding rods 448 are fixedly connected to the bottom surface of the two ends of the square plate 447, and a plurality of air nozzles 449 are fixedly connected to the inside of the square plate 447 and arranged in the air pipes at the bottom of the spray head 446, and a chamfer is arranged at the end of the lower protruding rod 448.

[0069] Further, during the up-down movement of the lifting plate 432, the protruding block 434 moves in the second sliding groove 4410, drives the frame plate 441 to drive the piston plate 443, moves left and right in the air cylinder 444 through the round rod 442, sends the gas into the spray head 446 through the hose 445, and blows the gas to the holes in the hole plate 300 through the air pipes, so as to eliminate the unbroken bubbles, and at the same time, when the groove 212 on the platform 210 moves to the lower side of the lower protruding rod 448, the bottom end falls into the groove 212 under the action of gravity, drives the square plate 447 to slide down in the first sliding groove 4131, and then makes the air nozzles 449 deeply enter the holes of the hole plate 300, so that the gas flow is close to the bubbles, so as to ensure the elimination of the bubbles, and the setting makes the gas flow act on the bubble gathering area at a close distance, realizes local strengthening and removal of stubborn bubbles, improves the elimination rate of the adhesive bubbles, and provides a reliable basis for statistical analysis of the PSMB5 phenotype influence.

[0070] The detection equipment of the bladder cancer metabolomics marker of the application is used for determining the influence of PSMB5 on the phenotype of bladder urothelial carcinoma cells.

[0071] Then, the cells are inoculated in a sterile 96-well plate, the cell concentration is adjusted to a suitable density by using complete culture medium, it is ensured that the cell densities of the groups in the same experiment must be consistent, and a blank control group hole (zero setting hole) is set, complete culture medium without cells is added to each hole for deducting the background absorbance, the holes are shaken gently to make the cells uniformly distributed, and group marks are made on the edges of the hole plate 300 cover.

[0072] Place the hole plate 300 in a 37°C, 5% CO2 incubator for about 12-24 hours to allow the cells to fully adhere and adapt to the environment;

[0073] Subsequently, in a sterile EP tube or reagent bottle, mix the CCK8 reagent with fresh complete medium at a ratio of 1:9, remove the hole plate 300 from the incubator, carefully aspirate the old culture medium in each hole with a pipette, add fresh complete medium to the experimental group holes and blank control group holes, ensure that the cells are immersed, then add the premixed CCK8 working solution to each hole;

[0074] Next, remove the hole plate 300 and place it inside the placement groove 211 of the platform 210, start the enzyme marker 100, set the parameters, and then return the moving platform 200 to its interior to detect the hole plate 300;

[0075] During the movement of the moving platform 200, the toothed plate 220 engages the gear 422, driving the dial plate 421 to rotate. When the lever 423 comes into contact with the bottom horizontal plate of the bent plate 431, it drives it to move upwards and separates from it. After the spring 436 is retracted, it is pushed downwards by its own elastic force, causing the bent plate 431 to fall back into place. The knocking rod 433 knocks the surface of the hole plate 300, causing the bubbles to detach from the hole wall and float upwards to break;

[0076] During the upward and downward movement of the lifting plate 432, the protrusion 434 moves within the second sliding groove 4410, driving the frame plate 441 to move the piston plate 443 left and right within the air cylinder 444 through the round rod 442. The gas is sent into the nozzle 446 through the hose 445 and blown into the holes of the hole plate 300 through several gas pipes, eliminating unbroken bubbles;

[0077] At the same time, when the groove 212 on the platform 210 moves below the lower protrusion 448, its bottom end falls into the groove 212 under its own gravity, driving the square plate 447 to slide downward within the first sliding groove 4131, and then allowing several air nozzles 449 to penetrate into the holes of the hole plate 300, allowing the gas flow to approach the bubbles, thereby ensuring the elimination of bubbles;

[0078] After the enzyme marker 100 completes the detection, statistical software is used for comparison between multiple groups, and statistical analysis is performed on the detection data.

[0079] The foregoing description of specific exemplary embodiments of the application is intended for purposes of illustration and example only. These descriptions are not intended to limit the application in any way. It is clear that many changes and modifications can be made to the application as described above without departing from the spirit and scope of the application. The exemplary embodiments are chosen and described in order to explain the principles of the application and its practical application, so that those skilled in the art can implement and utilize the various exemplary embodiments of the application and various modifications and changes. The scope of the application is intended to be defined by the specification and its equivalents.

Claims

1. A device for detecting bladder cancer metabolomics markers comprising a microplate reader, a mobile station and a well plate placed inside it, characterized in that: The device further comprises a bubble remover arranged on the outer wall of the enzyme marker, the bubble remover comprises a fixed part, a pair of transmission parts, a knocking part and a gas blowing part arranged in the fixed part; The moving table comprises a table plate and tooth plates arranged on the left and right sides of the table plate; The transmission part comprises a dial, a gear arranged on the end of the central shaft of the dial, and a dial rod arranged on the outer wall of the dial near the edge, when the table plate is retracted into the enzyme marker, the tooth plate engages the gear to drive the dial to rotate; The knocking part comprises a pair of bent plates, a lifting plate arranged on the top surface of the pair of bent plates, a plurality of knocking rods distributed on the bottom surface of the lifting plate, and a protrusion arranged on the front wall of the lifting plate, when the dial rotates, the dial rod contacts the bottom transverse plate of the bent plate, drives it to move upward, and after the dial rod is separated from the bent plate, the bent plate falls back to the original position, the knocking rod knocks the surface of the hole plate, so that the bubbles are separated from the hole wall and float up to be broken; The gas blowing part comprises a frame plate, a piston plate moving with the frame plate, a gas cylinder sleeved outside the piston plate, and a nozzle provided with a plurality of gas pipes at the bottom, a second sliding groove is formed in the inner part of the frame plate, the protrusion moves in the second sliding groove, drives the frame plate to move the piston plate left and right in the gas cylinder, and the gas is sent into the nozzle through the pipeline and blown into the hole of the hole plate through the gas pipes to eliminate the unbroken bubbles; A placing groove for placing the hole plate is formed on the top surface of the table plate, a plurality of recesses for providing a falling interval for the internal structure of the gas blowing part are arranged on the left and right sides of the placing groove on the top surface of the table plate; The fixed part comprises a fixed box, an upper partition plate clamped on the inner wall of the fixed box, and an inner partition plate clamped and fixed on the bottom surface of the upper partition plate on the left and right sides; A rotating groove is formed on the outer wall of the inner partition plate, and a first sliding groove for providing a sliding interval for the internal structure of the gas blowing part is formed on the inner wall thereof; A square plate is further arranged below the nozzle and is slidingly connected to the inside of the first sliding groove, lower protruding rods are clamped on the bottom surface of the two ends of the square plate, a plurality of gas nozzles are clamped and fixed in the gas pipes at the bottom of the nozzle inside the square plate, and a chamfer is formed on the end of the lower protruding rod.

2. The device for detecting bladder cancer metabolomic markers according to claim 1, characterized in that: The table plate is clamped and fixed with an electric push rod inside the enzyme marker for controlling the movement thereof, and the tooth plate is fixedly connected to the outer wall of the table plate by a screw.

3. The device for detecting bladder cancer metabolomic markers according to claim 1, characterized in that: Upper and lower through grooves are formed on the left and right ends of the upper partition plate.

4. The device for detecting bladder cancer metabolomic markers according to claim 1, characterized in that: The dial is rotatably connected to the inside of the rotating groove, the end of the central shaft of the dial extends to the inside of the inner partition plate, the gear is clamped and fixed on the end of the central shaft of the dial, and the dial rod is integrally formed with the dial.

5. The device for detecting bladder cancer metabolomic markers according to claim 1, characterized in that: The end of the transverse plate of the bent plate extends to the center of the dial, the bottom surface is above the dial rod, the end of the longitudinal plate body of the bent plate is clamped on the bottom surface of the lifting plate, and the knocking rod and the protrusion are both hot melt connected to the outer wall of the lifting plate.

6. The device for detecting bladder cancer metabolomic markers according to claim 1, characterized in that: A plurality of regularly distributed telescopic rods and springs sleeved outside the telescopic rods are clamped and fixed on the top surface of the bent plate, the top end of the telescopic rod is clamped and fixed on the inner top surface of the fixed box, and the spring provides an elastic force to push the lifting plate to move downward.

7. The device for detecting bladder cancer metabolomic markers according to claim 1, characterized in that: The frame plate is slidingly connected to the top surface of the upper partition plate, a round rod is clamped and fixed between the end of the frame plate and the piston plate, and the piston plate is slidingly connected to the inside of the gas cylinder.

8. The device for detecting bladder cancer metabolomic markers according to claim 1, characterized in that: The air cylinder is fixedly connected to the top surface of the upper partition plate by a screw, upper and lower ends of the air cylinder are respectively provided with an air inlet valve and an air outlet valve, a hose is clamped to the outer side of the air outlet valve, and the other end of the hose is clamped and fixed with the spray head.

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