Additive for relieving stress of laying hens in egg laying peak period, application and feed

Abstract

The invention belongs to the technical field of livestock breeding, and discloses an additive for relieving stress of laying hens in the egg laying peak period, application and feed.The additive for relieving the stress of the laying hens in the egg laying peak period is prepared from, by weight, 20-50 parts of selenium homolanonine, 100-110 parts of 25-hydroxy vitamin D3, 150-200 parts of compound enzyme and 50-200 parts of lactobacillus plantarum. The invention discloses an additive for relieving stress of laying hens in an egg-laying peak period, aiming at the problem that a reproductive system of the laying hens in the egg-laying peak period is opened and exposed in the environment and is easily influenced by various stress factors such as the environment and the like, and effectively improving the anti-stress capability of the laying hens in the egg-laying peak period.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of livestock breeding, in particular to an additive for relieving stress of laying hens in the peak egg production period, application and feed. BACKGROUND

[0002] The number of laying hens in China has remained above 1.5 billion per year, and more than 70% of them are raised in large-scale farms. Based on this, modern egg production technology has formed a highly intensive and standardized industrial system, the core of which is to achieve efficient production through nutritional regulation, environmental control and biological safety measures. One direction of nutritional regulation is selenium supplementation. Selenium, as the core component of glutathione peroxidase, can significantly improve the antioxidant capacity of laying hens, reduce the damage of oxidative stress to the liver and reproductive system, and enhance immunity, making the antibody level of avian influenza increase by 25% and the laying period extend by 20-30 days.

[0003] The current selenium supplement in the prior art mainly includes: amino acid chelated selenium such as selenium yeast, selenomethionine and selenium lysine, natural selenium-containing extract such as selenium-rich algae and selenium malt, selenium ester polysaccharide and synthetic organic selenium selenoprotein, and inorganic selenium. Organic selenium has become the mainstream due to its high activity and the advantage of directly participating in chicken metabolism. However, not all organic selenium can be used as a selenium supplement for chicken feed. Different organic seleniums have different effects on chickens, and even some organic seleniums are difficult for chickens to absorb due to their structural problems.

[0004] Prior art 1: Chinese patent application 201510878476.X discloses a kind of rich organic selenium egg feed additive, which is made of the following raw materials: selenium-rich purple sweet potato, mushroom leftovers, seafood leftovers, garlic, selenium-rich tea leaves, selenium-rich yeast powder, selenomethionine, Potamogeton ogonki, Astragalus, sedge, purple yam, lophatherum gracile, plumeria alba, fragrant grass, gangmei root, semen astragali complanati, eustegia japonica, gomphrena globosa, snail, fish eye grass, grape wine yeast agent, bifidobacterium agent, vitamin E and citric acid iron.

[0005] Prior art 1 is to form a selenium supplement by compounding selenium-rich purple sweet potato, selenium-rich tea leaves, selenium-rich yeast powder, selenomethionine, etc., so as to reduce the absorption barrier intensity after the chicken consumes it and improve the absorption of laying hens. However, prior art 1 mainly studies the aspects of significantly improving the thickness of eggshell, egg weight and selenium content, reducing the breakage rate, and increasing the nutritional value after supplementing selenium to laying hens, so as to effectively increase the selenium content of eggs. It does not further study and consider the needs of laying hens during the egg production process.

[0006] Laying hens face factors such as nutritional conversion, environmental stress, and disease risk at different growth and production stages, which affect the normal performance of laying hens. Laying hens produce eggs every day, which is equivalent to a state of frequent reproduction, and the reproductive system is always inflamed. Therefore, blood regulation and inflammation reduction are important issues for laying hens.

[0007] The prior art 1 improves the quality of eggs by compounding various components containing selenium, and does not study the anti-stress ability of laying hens during the peak egg production period, therefore, it is necessary to develop a feed additive which can not only improve the selenium content of eggs, but also effectively improve the anti-stress ability of laying hens during the peak egg production period, so as to reduce the inflammatory effect on the reproductive system of laying hens. SUMMARY

[0008] One of the purposes of the present application is to provide an additive for relieving stress of laying hens during the peak egg production period, which is compounded by various different substances, can not only effectively improve the biological conversion rate of selenium, but also effectively improve the anti-stress ability of laying hens during the peak egg production period, so as to reduce the inflammatory effect on the reproductive system of laying hens.

[0009] Another purpose of the present application is to provide a use of the additive for relieving stress of laying hens during the peak egg production period, for preparing feed, which can effectively improve the stress relieving ability of laying hens during the peak egg production period, and effectively improve the quality of eggs.

[0010] Meanwhile, the present application also provides a feed for relieving stress of laying hens during the peak egg production period, which uses the additive for relieving stress of laying hens during the peak egg production period of the present application as a selenium supplement and a component for improving the anti-stress ability of laying hens, which can effectively relieve the stress of laying hens during the peak egg production period.

[0011] To achieve the above purpose, the present application provides an additive for relieving stress of laying hens during the peak egg production period, which comprises 20-50 parts of selenomethionine, 100-110 parts of 25-hydroxyvitamin D3, 150-200 parts of complex enzyme and 50-200 parts of lactobacillus plantarum by weight.

[0012] Lactobacillus plantarum can improve the proportion of intestinal beneficial bacteria, improve intestinal health, and fully utilize the prebiotics released by enzyme preparations.

[0013] 25-hydroxyvitamin D3 can improve the absorption of calcium in high-yield laying hens, promote bone development and increase bone strength, increase egg production rate, increase eggshell thickness, and reduce broken egg rate by 15%.

[0014] Compared with traditional yeast selenium and other selenium sources, selenomethionine has obvious advantages in water solubility, absorption and conversion efficiency, metabolic deposition efficiency and safety, etc., improves the bioavailability of selenium, better deposits in eggs, and improves the anti-stress ability and immune function of laying hens.

[0015] Compared with other periods of laying hens, the egg laying peak period or high-yield laying hens are different from other stages: a large amount of egg laying requires a large amount of energy consumption, and if the energy is insufficient, it needs to be compensated by the body consumption; secondly, the reproductive system is exposed to the environment during the egg laying period, and is easily affected by various stress factors in the environment to cause the production performance to decrease; and the present application further improves the stress resistance of laying hens by compounding high lanthionine selenium, 25-hydroxy vitamin D3, complex enzymes and lactobacillus plantarum to comprehensively improve the intestinal tract, energy supplement, nutrient absorption and other aspects of the laying hens, thereby effectively relieving the stress of the laying hens during the egg laying peak period; and improving the selenium biological conversion capacity of the laying hens, so that the eggs obtained have a higher selenium content.

[0016] In the technical solution of the present application, the complex enzyme can be combined with various enzymes capable of effectively promoting nutrient absorption, reducing intestinal viscosity, improving microbial flora and saving energy consumption to form a complex enzyme.

[0017] Preferably, the complex enzyme comprises at least two of endo-1,3(4)-beta-glucanase, endo-1,4-beta-xylanase and endo-1,4-beta-mannanase.

[0018] More preferably, the complex enzyme comprises endo-1,3(4)-beta-glucanase, endo-1,4-beta-xylanase and endo-1,4-beta-mannanase, and the weight ratio of the endo-1,3(4)-beta-glucanase, endo-1,4-beta-xylanase and endo-1,4-beta-mannanase is 10-30:10-30:10-30.

[0019] The functions of glucanase and xylanase: promote nutrient absorption, help open the cell wall of plant raw materials, release more nutrients; at the same time, reduce the intestinal viscosity, increase the contact area of feed raw materials and digestive juice, realize the functions of promoting the full release of nutrients in feed raw materials and improving nutrient absorption; improve the intestinal health, release prebiotics-xylo-oligosaccharides when opening the cell wall of plant raw materials, xylo-oligosaccharides promote the proliferation of lactic acid and butyric acid bacteria on the one hand, produce short-chain fatty acids, and on the other hand, promote the integrity of intestinal epithelial cells, improve the microbial flora, and realize the function of improving intestinal health.

[0020] The function of mannanase: eliminate the sticky effect caused by mannan, promote the digestion and absorption of nutrients; hydrolyze beta-mannan to release energy; eliminate the innate immune response induced by beta-mannan in feed to save energy consumption; generate mannan oligosaccharides to inhibit the proliferation of pathogenic bacteria.

[0021] The present application also includes a use of an additive for preparing a feed for relieving the stress of laying hens during the egg laying peak period.

[0022] Further, the application discloses a feed for relieving stress of laying hens in an egg production peak period, which contains 0.4-0.5 wt% of the additive for relieving stress of laying hens in an egg production peak period.

[0023] Beneficial effects

[0024] Compared with the prior art, the application discloses an additive for relieving stress of laying hens in an egg production peak period, which effectively improves the stress resistance of the laying hens in the egg production peak period in view of the problem that the reproductive system of the laying hens in the egg production peak period is exposed to the environment and is easily affected by various stress factors. DETAILED DESCRIPTION

[0025] The application will be further described below in combination with examples, but does not constitute any limitation to the application, and any limited modification made within the scope of the claims of the application is still within the scope of the claims of the application.

[0026] In order to specifically explain the technical content of the application, the following further explains in combination with the embodiments.

[0027] In the following examples and comparative examples, the parts and % are weight parts and weight percentages, respectively, unless otherwise specified.

[0028] In the following examples and comparative examples, the high lanthionine selenium is purchased from AB Tor-Sel of Yinglian Pumeixin Technology (Jiangxi) Co., Ltd., which contains ≥85% high lanthionine selenium; the methionine selenium is purchased from Yinglian Pumeixin Technology (Jiangxi) Co., Ltd.; the 25-hydroxy vitamin D3 is purchased from Yinglian Pumeixin Technology (Jiangxi) Co., Ltd.; the endo-1, 3(4)-β-glucanase is purchased from Yinglian Pumeixin Technology (Jiangxi) Co., Ltd.; the endo-1, 4-β-xylanase is purchased from Yinglian Pumeixin Technology (Jiangxi) Co., Ltd.; the endo-1, 4-β-mannanase is purchased from Yinglian Pumeixin Technology (Jiangxi) Co., Ltd.; and the lactobacillus plantarum is purchased from Tibet Angel Zhubeng Biological Technology Co., Ltd.

[0029] Example 1

[0030] The additive for relieving stress of laying hens in an egg production peak period comprises 35 parts of high lanthionine selenium, 100 parts of 25-hydroxy vitamin D3, 50 parts of endo-1, 3(4)-β-glucanase, 50 parts of endo-1, 4-β-xylanase, 50 parts of endo-1, 4-β-mannanase and 50 parts of lactobacillus plantarum.

[0031] Example 2

[0032] The example is basically the same as example 1, except that the high lanthionine selenium is 50 parts.

[0033] Example 3

[0034] The same as example 1, except that the homoarginine selenium is 20 parts and the 25-hydroxyvitamin D3 is 110 parts.

[0035] Example 4

[0036] An additive for relieving stress of laying hens in the egg-laying peak period, comprising 35 parts of homoarginine selenium, 100 parts of 25-hydroxyvitamin D3, 75 parts of endo-1, 3(4)-β-glucanase, 75 parts of endo-1, 4-β-xylanase and 50 parts of Lactobacillus plantarum.

[0037] Comparative Example 1

[0038] An additive, comprising 50 parts of homoarginine selenium, 71 parts of endo-1, 3(4)-β-glucanase, 71 parts of endo-1, 4-β-xylanase, 71 parts of endo-1, 4-β-mannanase and 72 parts of Lactobacillus plantarum.

[0039] Comparative Example 2

[0040] The same as example 1, except that the homoarginine selenium is replaced by methionine selenium.

[0041] Application of the additive

[0042] The additives of examples 1-4 and comparative examples 1-2 are respectively added into the basic diet of laying hens according to the corresponding concentration requirements in table 1 to configure application examples 1-4 and application comparative examples 1-2, and are used to feed the laying hens; the blank control group is fed with the basic diet of laying hens containing 0.4% inorganic selenium; the laying hens are pre-fed for 1 week, and then the test is conducted for 4 weeks.

[0043] Table 1: Feed formulation table

[0044] Composition Amount Additive for relieving stress of egg-laying peak period of laying hens 0.4％ Laying hen basic daily ration 99.6％

[0045] The basic diet of laying hens is formulated as shown in table 2 below:

[0046] Table 2: Basic diet formulation table of laying hens

[0047] Composition Supplier Amount Corn 7.0 Jingzhou Liyuan Feedstuff Co., Ltd. 60.915 Brans Jingzhou Liyuan Feedstuff Co., Ltd. 3.300 Soybean meal 43 (crude) Jingzhou Liyuan Feedstuff Co., Ltd. 23.900 Stone powder (granule) Jingzhou Liyuan Feedstuff Co., Ltd. 10.010 Dicalcium phosphate type III Jingzhou Liyuan Feedstuff Co., Ltd. 1.008 L-methionine Jingzhou Liyuan Feedstuff Co., Ltd. 0.110 Lysine 70% Jingzhou Liyuan Feedstuff Co., Ltd. 0.140 Choline chloride Jingzhou Liyuan Feedstuff Co., Ltd. 0.100 Vitamin C Jingzhou Liyuan Feedstuff Co., Ltd. 0.020 Sodium chloride Jingzhou Liyuan Feedstuff Co., Ltd. 0.300 Xylanase Jingzhou Liyuan Feedstuff Co., Ltd. 0.010 Phytase Jingzhou Liyuan Feedstuff Co., Ltd. 0.020 Beta-mannanase Jingzhou Liyuan Feedstuff Co., Ltd. 0.005 Dextranase Jingzhou Liyuan Feedstuff Co., Ltd. 0.005 Sodium bicarbonate Jingzhou Liyuan Feedstuff Co., Ltd. 0.150 Cantharides yellow 10% Jingzhou Liyuan Feedstuff Co., Ltd. 0.007

[0048] Performance test

[0049] I. Stress experiment of laying hens in the egg-laying peak period

[0050] On the last day of the 4th week of the experiment, after the laying hens were deprived of food for 12 hours, one laying hen (close to the average weight) was selected from each replicate, 9 ml of blood was taken from the jugular vein of each laying hen (two tubes without anticoagulant), and the serum was separated by centrifugation at 3500 rpm for 15 minutes, and the separated serum was divided into 5 centrifuge tubes and stored in a -20°C refrigerator for testing. The serum antioxidant indicators were determined according to the specific methods of the corresponding kits to determine the total glutathione content, glutathione peroxidase content, superoxide dismutase content, and malondialdehyde content. The kits were purchased from Nanjing Jiancheng Bioengineering Co., Ltd.

[0051] According to the above scheme, the blank control group, application examples 1-4 and application comparative examples 1-2 were tested, and the results are shown in Table 3.

[0052] Table 3: Results of stress test of laying hens in the peak egg production period of application examples 1-4 and application comparative example 1

[0053]

[0054] According to the results in Table 3, it can be seen that:

[0055] According to the comparison of the data of application examples 1-4 and the blank control group, it can be seen that the technical scheme of the present application, which uses high lanthionine selenium, 25-hydroxyvitamin D3, complex enzymes and lactobacillus plantarum, can effectively improve the stress resistance of laying hens and significantly increase the content of glutathione peroxidase and superoxide dismutase.

[0056] According to the comparison of the data of application example 1 and application comparative example 1, it can be seen that in the technical scheme of the present application, the lack of 25-hydroxyvitamin D3 significantly reduces the content of glutathione peroxidase and superoxide dismutase. Based on the role of 25-hydroxyvitamin D3, it is not to improve the stress resistance but to improve the calcium absorption capacity of laying hens. It is speculated that it has a synergistic effect with other components in the present application to improve the stress resistance of the additive.

[0057] According to the comparison of the data of application example 1 and application comparative example 2, it can be seen that when using methionine selenium, which is also an organic selenium, to replace high lanthionine selenium, it is found that the content of glutathione peroxidase and superoxide dismutase decreases significantly, which is speculated to have two reasons:

[0058] (1) The stress resistance of high lanthionine selenium is significantly higher than that of methionine selenium;

[0059] (2) High lanthionine selenium has a synergistic effect with other components in the additive.

[0060] Combined with the data results of application comparative example 1, it can be reasonably inferred that in the technical scheme of the present application, high lanthionine selenium and 25-hydroxyvitamin D3 have a synergistic effect to improve the stress resistance of the additive.

[0061] II. Selenium content experiment of eggs

[0062] On the 0th, 7th, 14th, 21st, and 28th day of the formal test, 30 eggs were selected from each group, and the yolk of every 10 eggs was fully mixed to make an egg sample. The selenium content in the eggs was determined according to the National Food Safety Standard for Determination of Selenium in Food (GB 5009.93-2017).

[0063] According to the above scheme, the blank control group, application examples 1-4, and application comparative examples 1-2 were tested, and the results are shown in Table 4.

[0064] Table 4 Selenium content results of eggs in application examples 1-4 and application comparative example 1

[0065]

[0066] According to the results in Table 4, it can be seen that:

[0067] According to the comparison of the data of application examples 1-4 and the blank control group, it can be seen that the technical scheme of the present application can effectively improve the selenium content in eggs.

[0068] According to the data of application comparative example 1, it can be seen that the additive of the present application lacks 25-hydroxyvitamin D3, although the amount of other components is added, the selenium content of the eggs is significantly decreased. However, compared with application comparative example 2, the selenium content of the eggs of application comparative example 1 is still significantly improved, which proves that the high lanthionine selenium used in the present application can significantly improve the absorption and conversion of selenium by laying hens, thereby improving the selenium content in eggs. It can also be inferred that in the technical scheme of the present application, 25-hydroxyvitamin D3 plays a role in improving the absorption and conversion of high lanthionine selenium by laying hens.

[0069] III. Selenium deposition detection in breast meat of laying hens

[0070] The determination of selenium content in chicken meat adopts GB 5009.93-2017 National Food Safety Standard for Determination of Selenium in Food.

[0071] According to the above scheme, the blank control group, application examples 1-4, and application comparative examples 1-2 were tested, and the results are shown in Table 5.

[0072]

[0073]

[0074] According to the results in Table 5, it can be seen that:

[0075] According to the data comparison of application examples 1-4 and the blank control group, the technical scheme of the present application can effectively improve the selenium content in eggs;

[0076] According to the data of application comparative example 1, it can be known that the additive of the present application has a significant decline in the improvement range of the selenium content of eggs in the absence of 25-hydroxyvitamin D3, although the use amount of other components is added. However, compared with application comparative example 2, the selenium content of the eggs of application comparative example 1 is still obviously improved, which proves that the high lanthionine selenium used in the present application can significantly improve the absorption and conversion of selenium by laying hens, thereby improving the total selenium content and selenium content of breast meat. At the same time, it can also be inferred that in the technical scheme of the present application, 25-hydroxyvitamin D3 plays a role in improving the absorption and conversion of high lanthionine selenium by laying hens.

[0077] The embodiments presented herein are only selected from combinations of all possible embodiments. The appended claims should not be limited by the description of the embodiments of the present application. Some numerical ranges used in the claims include sub-ranges within them, and variations in these ranges should also be covered by the appended claims.

Claims

1. An additive for relieving stress in laying hens during peak egg-laying period, characterized in that: Calculated by weight, the invention comprises 20-50 parts of homolanolinic acid selenium, 100-110 parts of 25-hydroxyvitamin D3, 150-200 parts of complex enzyme and 50-200 parts of plant lactobacillus.

2. The additive for alleviating stress during the peak egg-laying period of laying hens according to claim 1, characterized in that The complex enzyme comprises at least two of endo-1,3(4)-β-glucanase, endo-1,4-β-xylanase and endo-1,4-β-mannanase.

3. Use of the additive for alleviating stress during the peak egg-laying period of laying hens as claimed in claim 1 or 2 in preparing feed.

4. A feed for relieving stress in laying hens during the peak egg-laying period, characterized in that: The invention contains 0.4-0.5 wt% of the additive for relieving stress of laying hens during the peak egg-laying period as claimed in claim 1 or 2.

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